G2C::Behaviour

 

Overview: Effect sizes (Cohen d) for 16 behaviour variables

Mutant mice showed large overall behavioural difference from wildtypes, with six of 16 behaviour variables significantly impacted in these mutant mice. In the elevated plus maze task, two behavioural variables of five were significantly impacted in mutants. In the rotarod task, one behavioural variable, RR learning, was significantly decreased in mutants. In the fear learning task, one behavioural variable, fear learning trial effect, was significantly decreased in mutants. In the contextual memory task, two behavioural variables of two were significantly impacted in mutants. Only open field/novel object exploration and cued memory tasks were unaffected. Definitions of variables may be found below.

The G2CMine data warehouse provides cohort summaries and individual mouse observations from the IRSp53 knock-out line phenotyping.

 

Variables shown are: EPM total distance, Total distance (cm) travelled in any arm or central zone of the EPM. EPM max speed, Maximum speed (cm/s) travelled in any arm or central zone of the EPM. EPM % time in open, Percentage of time in the open or closed arms of the EPM spent in open arms. EPM time in centre, Total time (s) spent in the central zone of the EPM. EPM max speed, open vs closed, Difference between the maximum speed (cm/s) observed in the open arms and the closed arms of the EPM. OF, NOE total distance, Total distance travelled (log₁₀ cm) during initial exposure to the open field and in presence of the novel object. NOE vs OF distance travelled, Difference in distance travelled (cm) in presence of the novel object and during initial exposure to open field. RR naive fall time, Fall time on accelerating rotarod (log₁₀ s), naive performance in session 1. RR learning, Learning on rotarod, measured as increase in fall time per trial (s/trial) in session 1. RR memory, Memory on rotarod, measured as excess fall time at middle of session 2 relative to middle of session 1. Fear learning, trial effect, Fear learning, measured as extra % time freezing before third trial compared to % time freezing before first trial. Fear learning, tone effect, Fear learning, measured as increase in % time freezing due to third tone compared to increase in % time freezing due to first tone. Contextual memory, mean, Contextual memory, measured as difference in % time freezing during first 120 s re-exposure to the box compared to first 120 s in the box on previous day. Contextual memory, change, Contextual memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the box. Cued memory, mean, Cued memory, measured as increase in % time spent freezing during 120 s of tone re-exposure compared to increase in % time spent freezing during initial tone on previous day. Cued memory, change, Cued memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the tone.

 

Variable	Units	Wildtype M (n=9)	Wildtype F (n=14)	Mutant M (n=8)	Mutant F (n=10)	P(sex×mutation)	P(mutation)
EPM total distance	cm	973 (82)	774 (45)	680 (115)	983 (87)	0.0031 **	0.98
EPM max speed	cm/s	19.9 (1.2)	15.4 (1)	13.5 (1.8)	16.7 (1.3)	0.0055 **	0.15
EPM % time in open	%	14.9 (5.5)	27.8 (4.8)	56.5 (11.2)	59.2 (7.1)	0.47	0.000009 ***
EPM time in centre	s	118 (12)	176 (13)	131 (24)	123 (16)	0.051	0.12
EPM max speed, open vs closed	cm/s	-9.8 (2.2)	-4.1 (0.6)	1 (2.1)	1.5 (1)	0.085	0.0000038 ***
OF, NOE total distance	log10 cm	3.21 (0.11)	3.16 (0.09)	3.21 (0.09)	3.41 (0.07)	0.19	0.13
NOE vs OF distance travelled	cm	-270 (110)	-200 (150)	-220 (160)	60 (290)	0.59	0.38
RR naive fall time	log10 s	1.01 (0.12)	1.16 (0.11)	1.08 (0.06)	1.08 (0.12)	0.52	0.88
RR learning	s/trial	1.2 (0.7)	4.4 (1.1)	0.2 (0.2)	-0.1 (0.6)	0.064	0.002 **
RR memory	s	6.3 (5.4)	6 (3.5)	7.2 (4.1)	0.2 (3.7)	0.43	0.48
Fear learning, trial effect	% freezing	56.5 (9.2)	69 (4.2)	48.7 (9)	40.6 (8.3)	0.18	0.011 *
Fear learning, tone effect	% freezing	-2.9 (9.7)	-0.3 (6.7)	-8.8 (6.7)	-11.2 (5.4)	0.74	0.24
Contextual memory, mean	% freezing	38.6 (5.5)	50.5 (5.6)	23.1 (6.3)	18.1 (4.1)	0.14	0.000056 ***
Contextual memory, change	% freezing	41.6 (5.8)	30.7 (9.4)	-7.5 (8.8)	11.2 (6.4)	0.093	0.00056 ***
Cued memory, mean	% freezing	1.9 (5.4)	-4 (7.3)	-1.3 (4.3)	-1.3 (4.5)	0.65	0.97
Cued memory, change	% freezing	-9.9 (9.1)	-0.5 (4.6)	-11.1 (9.2)	-15.1 (6.7)	0.36	0.21

 

Elevated Plus Maze Performance

 

Variables shown are: EPM total distance, Total distance (cm) travelled in any arm or central zone of the EPM. EPM max speed, Maximum speed (cm/s) travelled in any arm or central zone of the EPM. EPM % time in open, Percentage of time in the open or closed arms of the EPM spent in open arms. EPM time in centre, Total time (s) spent in the central zone of the EPM. EPM max speed, open vs closed, Difference between the maximum speed (cm/s) observed in the open arms and the closed arms of the EPM.

 

Variable	Units	Wildtype M (n=9)	Wildtype F (n=14)	Mutant M (n=8)	Mutant F (n=10)	P(sex×mutation)	P(mutation)
EPM total distance	cm	973 (82)	774 (45)	680 (115)	983 (87)	0.0031 **	0.98
EPM max speed	cm/s	19.9 (1.2)	15.4 (1)	13.5 (1.8)	16.7 (1.3)	0.0055 **	0.15
EPM % time in open	%	14.9 (5.5)	27.8 (4.8)	56.5 (11.2)	59.2 (7.1)	0.47	0.000009 ***
EPM time in centre	s	118 (12)	176 (13)	131 (24)	123 (16)	0.051	0.12
EPM max speed, open vs closed	cm/s	-9.8 (2.2)	-4.1 (0.6)	1 (2.1)	1.5 (1)	0.085	0.0000038 ***

 

Open Field and Novel Object Exploration Performance

 

Variables shown are: OF, NOE total distance, Total distance travelled (log₁₀ cm) during initial exposure to the open field and in presence of the novel object. NOE vs OF distance travelled, Difference in distance travelled (cm) in presence of the novel object and during initial exposure to open field.

 

Variable	Units	Wildtype M (n=9)	Wildtype F (n=14)	Mutant M (n=8)	Mutant F (n=10)	P(sex×mutation)	P(mutation)
OF, NOE total distance	log10 cm	3.21 (0.11)	3.16 (0.09)	3.21 (0.09)	3.41 (0.07)	0.19	0.13
NOE vs OF distance travelled	cm	-270 (110)	-200 (150)	-220 (160)	60 (290)	0.59	0.38

 

Rotarod Performance

 

Variables shown are: RR naive fall time, Fall time on accelerating rotarod (log₁₀ s), naive performance in session 1. RR learning, Learning on rotarod, measured as increase in fall time per trial (s/trial) in session 1. RR memory, Memory on rotarod, measured as excess fall time at middle of session 2 relative to middle of session 1.

 

Variable	Units	Wildtype M (n=9)	Wildtype F (n=14)	Mutant M (n=8)	Mutant F (n=10)	P(sex×mutation)	P(mutation)
RR naive fall time	log10 s	1.01 (0.12)	1.16 (0.11)	1.08 (0.06)	1.08 (0.12)	0.52	0.88
RR learning	s/trial	1.2 (0.7)	4.4 (1.1)	0.2 (0.2)	-0.1 (0.6)	0.064	0.002 **
RR memory	s	6.3 (5.4)	6 (3.5)	7.2 (4.1)	0.2 (3.7)	0.43	0.48

 

Fear Conditioning Performance

 

Variables shown are: Fear learning, trial effect, Fear learning, measured as extra % time freezing before third trial compared to % time freezing before first trial. Fear learning, tone effect, Fear learning, measured as increase in % time freezing due to third tone compared to increase in % time freezing due to first tone. Contextual memory, mean, Contextual memory, measured as difference in % time freezing during first 120 s re-exposure to the box compared to first 120 s in the box on previous day. Contextual memory, change, Contextual memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the box. Cued memory, mean, Cued memory, measured as increase in % time spent freezing during 120 s of tone re-exposure compared to increase in % time spent freezing during initial tone on previous day. Cued memory, change, Cued memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the tone.

 

Variable	Units	Wildtype M (n=9)	Wildtype F (n=14)	Mutant M (n=8)	Mutant F (n=10)	P(sex×mutation)	P(mutation)
Fear learning, trial effect	% freezing	56.5 (9.2)	69 (4.2)	48.7 (9)	40.6 (8.3)	0.18	0.011 *
Fear learning, tone effect	% freezing	-2.9 (9.7)	-0.3 (6.7)	-8.8 (6.7)	-11.2 (5.4)	0.74	0.24
Contextual memory, mean	% freezing	38.6 (5.5)	50.5 (5.6)	23.1 (6.3)	18.1 (4.1)	0.14	0.000056 ***
Contextual memory, change	% freezing	41.6 (5.8)	30.7 (9.4)	-7.5 (8.8)	11.2 (6.4)	0.093	0.00056 ***
Cued memory, mean	% freezing	1.9 (5.4)	-4 (7.3)	-1.3 (4.3)	-1.3 (4.5)	0.65	0.97
Cued memory, change	% freezing	-9.9 (9.1)	-0.5 (4.6)	-11.1 (9.2)	-15.1 (6.7)	0.36	0.21

Acquired (raw) data

• Animals
• Elevated Plus Maze
• Open Field
• Novel Object Exploration
• Rotarod
• Fear Conditioning

• Animals
• Elevated Plus Maze
• Open Field
• Novel Object Exploration
• Rotarod
• Fear Conditioning