G2C::Electrophysiology

NF1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices. With heterozygous genotype and no electrophysiological variables significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the NF1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 23 (9) 3160 (140) 37 (12) 3340 (110) 0.083 0.41
PPF at 50ms, ampl ratio % 12 (5) 179.7 (4.8) 17 (5) 167.8 (3.8) 0.077 0.16
Burst 1, PPF at 10ms, ampl ratio % 12 (5) 172 (7.2) 17 (5) 166.2 (5) 0.0026 ** 0.66
Burst 1, EPSP3 depr, ampl ratio % 12 (5) 62.8 (4.2) 17 (5) 68.2 (2.6) 0.084 0.39
Burst 1, ave amplitude µV 12 (5) 1225 (65) 17 (5) 1294 (56) 0.23 0.5
Burst 2, ave amplitude µV 12 (5) 2013 (97) 17 (5) 2098 (63) 0.48 0.46
Burst 3, ave amplitude µV 12 (5) 2263 (99) 17 (5) 2294 (70) 0.5 0.8
Burst 4, ave amplitude µV 12 (5) 2297 (108) 17 (5) 2313 (67) 0.46 0.9
Burst 5, ave amplitude µV 12 (5) 2259 (110) 17 (5) 2271 (65) 0.54 0.92
Burst 6, ave amplitude µV 12 (5) 2189 (105) 17 (5) 2199 (66) 0.47 0.94
Burst 7, ave amplitude µV 12 (5) 2123 (107) 17 (5) 2132 (67) 0.4 0.95
Burst 8, ave amplitude µV 12 (5) 2061 (108) 17 (5) 2057 (65) 0.34 0.98
Burst 9, ave amplitude µV 12 (5) 1996 (105) 17 (5) 2005 (65) 0.35 0.95
Burst 10, ave amplitude µV 11 (5) 1966 (106) 17 (5) 1953 (65) 0.34 0.92
Burst 2-4 ave amplitude vs Burst 1 % 12 (5) 180.3 (7) 17 (5) 174.6 (5.5) 0.048 * 0.64
Burst 8-10 ave amplitude vs Burst 2-4 % 11 (5) 91.9 (1.6) 17 (5) 89.5 (1.2) 0.33 0.28
LTP vs PTP, amplitude ratio % 12 (5) 78.8 (1.4) 17 (5) 76.9 (1.6) 0.31 0.46
LTP based on amplitude % 12 (5) 175.2 (9.7) 17 (5) 154.8 (5.4) 0.026 * 0.18

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 23 (9) 3160 (140) 37 (12) 3340 (110) 0.083 0.41

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 12 (5) 179.7 (4.8) 17 (5) 167.8 (3.8) 0.077 0.16

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 12 (5) 172 (7.2) 17 (5) 166.2 (5) 0.0026 ** 0.66
Burst 1, EPSP3 depr, ampl ratio % 12 (5) 62.8 (4.2) 17 (5) 68.2 (2.6) 0.084 0.39
Burst 1, ave amplitude µV 12 (5) 1225 (65) 17 (5) 1294 (56) 0.23 0.5
Burst 2, ave amplitude µV 12 (5) 2013 (97) 17 (5) 2098 (63) 0.48 0.46
Burst 3, ave amplitude µV 12 (5) 2263 (99) 17 (5) 2294 (70) 0.5 0.8
Burst 4, ave amplitude µV 12 (5) 2297 (108) 17 (5) 2313 (67) 0.46 0.9
Burst 5, ave amplitude µV 12 (5) 2259 (110) 17 (5) 2271 (65) 0.54 0.92
Burst 6, ave amplitude µV 12 (5) 2189 (105) 17 (5) 2199 (66) 0.47 0.94
Burst 7, ave amplitude µV 12 (5) 2123 (107) 17 (5) 2132 (67) 0.4 0.95
Burst 8, ave amplitude µV 12 (5) 2061 (108) 17 (5) 2057 (65) 0.34 0.98
Burst 9, ave amplitude µV 12 (5) 1996 (105) 17 (5) 2005 (65) 0.35 0.95
Burst 10, ave amplitude µV 11 (5) 1966 (106) 17 (5) 1953 (65) 0.34 0.92
Burst 2-4 ave amplitude vs Burst 1 % 12 (5) 180.3 (7) 17 (5) 174.6 (5.5) 0.048 * 0.64
Burst 8-10 ave amplitude vs Burst 2-4 % 11 (5) 91.9 (1.6) 17 (5) 89.5 (1.2) 0.33 0.28

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 12 (5) 78.8 (1.4) 17 (5) 76.9 (1.6) 0.31 0.46
LTP based on amplitude % 12 (5) 175.2 (9.7) 17 (5) 154.8 (5.4) 0.026 * 0.18

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT9
mutant12

 

Individual Mice

Mouse IDGenotype
wAf6440365eWT
wA6c2ae5facWT
wA411d5e724WT
wAfd77defaaWT
wA8d77d5ac7WT
wA64091321cWT
wA8bd30e9baWT
wA5b0d51463WT
wAcda0b7e8dWT
mAc3b050b7bmutant
mA77156b7c6mutant
mA77e145992mutant
mAca6c69ea5mutant
mAd6969adb7mutant
mAcebfc8537mutant
mA99d1d6880mutant
mAafb0e5320mutant
mA96d0a15dfmutant
mAef53ba2e4mutant
mA4b28d1988mutant
mAafd9591femutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA77156b7c6 mutant 1 38A 77156b7c6_slices_1-2-3.zip
mA77156b7c6 mutant 2 43A 77156b7c6_slices_1-2-3.zip
mA77156b7c6 mutant 3 38B 77156b7c6_slices_1-2-3.zip
mAd6969adb7 mutant 1 46A d6969adb7_slices_1-2-3.zip
mAd6969adb7 mutant 2 52A d6969adb7_slices_1-2-3.zip
mAd6969adb7 mutant 3 53B d6969adb7_slices_1-2-3.zip
mAc3b050b7b mutant 1 46A c3b050b7b_slices_1-2-3.zip
mAc3b050b7b mutant 2 54A c3b050b7b_slices_1-2-3.zip
mAc3b050b7b mutant 3 55B c3b050b7b_slices_1-2-3.zip
mA99d1d6880 mutant 2 41A 99d1d6880_slices_1-2-3.zip
mA99d1d6880 mutant 3 12B 99d1d6880_slices_1-2-3.zip
mAafb0e5320 mutant 1 28A afb0e5320_slices_1-2-3.zip
mAafb0e5320 mutant 2 53A afb0e5320_slices_1-2-3.zip
mAafb0e5320 mutant 3 44B afb0e5320_slices_1-2-3.zip
mA77e145992 mutant 1 58A 77e145992_slices_1-2-3.zip
mA77e145992 mutant 2 62A 77e145992_slices_1-2-3.zip
mA77e145992 mutant 3 15B 77e145992_slices_1-2-3.zip
mAef53ba2e4 mutant 1 46A ef53ba2e4_slices_1-2-3.zip
mAef53ba2e4 mutant 2 41A ef53ba2e4_slices_1-2-3.zip
mAef53ba2e4 mutant 3 61B ef53ba2e4_slices_1-2-3.zip
mA4b28d1988 mutant 1 47A 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 2 41A 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 3 12B 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 4 52B 4b28d1988_slices_1-2-3-4.zip
mAafd9591fe mutant 1 57A afd9591fe_slices_1-x-2-3.zip
mAafd9591fe mutant 2 55B afd9591fe_slices_1-x-2-3.zip
mAafd9591fe mutant 3 52B afd9591fe_slices_1-x-2-3.zip
mAca6c69ea5 mutant 1 31A ca6c69ea5_slices_x-1-2-3.zip
mAca6c69ea5 mutant 2 46A ca6c69ea5_slices_x-1-2-3.zip
mAca6c69ea5 mutant 3 54B ca6c69ea5_slices_x-1-2-3.zip
mA96d0a15df mutant 1 45A 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 2 52A 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 3 15B 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 4 54B 96d0a15df_slices_1-2-3-4.zip
mAcebfc8537 mutant 1 37A cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 2 62A cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 3 46B cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 4 41B cebfc8537_slices_1-2-3-4.zip
wAf6440365e WT 1 55A f6440365e_slices_1-2-3.zip
wAf6440365e WT 2 42A f6440365e_slices_1-2-3.zip
wAf6440365e WT 3 57B f6440365e_slices_1-2-3.zip
wAcda0b7e8d WT 1 46A cda0b7e8d_slices_1-2-3.zip
wAcda0b7e8d WT 2 52B cda0b7e8d_slices_1-2-3.zip
wAfd77defaa WT 1 46A fd77defaa_slices_1-2-3.zip
wAfd77defaa WT 2 52A fd77defaa_slices_1-2-3.zip
wAfd77defaa WT 3 55B fd77defaa_slices_1-2-3.zip
wA8bd30e9ba WT 1 46A 8bd30e9ba_slices_1-2-3.zip
wA8bd30e9ba WT 2 42A 8bd30e9ba_slices_1-2-3.zip
wA8bd30e9ba WT 3 53B 8bd30e9ba_slices_1-2-3.zip
wA8d77d5ac7 WT 1 87A 8d77d5ac7_slices_1-2-x-x.zip
wA8d77d5ac7 WT 2 42A 8d77d5ac7_slices_1-2-x-x.zip
wA5b0d51463 WT 1 87A 5b0d51463_slices_1-x-x-2.zip
wA5b0d51463 WT 2 62B 5b0d51463_slices_1-x-x-2.zip
wA64091321c WT 1 61A 64091321c_slices_1-x-2-x.zip
wA64091321c WT 2 43B 64091321c_slices_1-x-2-x.zip
wA411d5e724 WT 1 56A 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 2 52A 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 3 58B 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 4 41B 411d5e724_slices_1-2-3-4.zip
wA6c2ae5fac WT 1 12A 6c2ae5fac_slices_1-2-3-x.zip
wA6c2ae5fac WT 2 54A 6c2ae5fac_slices_1-2-3-x.zip
wA6c2ae5fac WT 3 56B 6c2ae5fac_slices_1-2-3-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA4b28d1988 mutant 1 47A 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 2 41A 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 3 12B 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 4 52B 4b28d1988_slices_1-2-3-4.zip
mAafd9591fe mutant 1 57A afd9591fe_slices_1-x-2-3.zip
mAafd9591fe mutant 2 55B afd9591fe_slices_1-x-2-3.zip
mAafd9591fe mutant 3 52B afd9591fe_slices_1-x-2-3.zip
mAca6c69ea5 mutant 1 31A ca6c69ea5_slices_x-1-2-3.zip
mAca6c69ea5 mutant 2 46A ca6c69ea5_slices_x-1-2-3.zip
mAca6c69ea5 mutant 3 54B ca6c69ea5_slices_x-1-2-3.zip
mA96d0a15df mutant 1 45A 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 2 52A 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 3 15B 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 4 54B 96d0a15df_slices_1-2-3-4.zip
mAcebfc8537 mutant 1 37A cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 2 62A cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 3 46B cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 4 41B cebfc8537_slices_1-2-3-4.zip
wA8d77d5ac7 WT 1 87A 8d77d5ac7_slices_1-2-x-x.zip
wA8d77d5ac7 WT 2 42A 8d77d5ac7_slices_1-2-x-x.zip
wA5b0d51463 WT 1 87A 5b0d51463_slices_1-x-x-2.zip
wA5b0d51463 WT 2 62B 5b0d51463_slices_1-x-x-2.zip
wA64091321c WT 1 61A 64091321c_slices_1-x-2-x.zip
wA64091321c WT 2 43B 64091321c_slices_1-x-2-x.zip
wA411d5e724 WT 1 56A 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 2 52A 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 3 58B 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 4 41B 411d5e724_slices_1-2-3-4.zip
wA6c2ae5fac WT 1 12A 6c2ae5fac_slices_1-2-3-x.zip
wA6c2ae5fac WT 2 54A 6c2ae5fac_slices_1-2-3-x.zip
wA6c2ae5fac WT 3 56B 6c2ae5fac_slices_1-2-3-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA4b28d1988 mutant 1 47A 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 2 41A 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 3 12B 4b28d1988_slices_1-2-3-4.zip
mA4b28d1988 mutant 4 52B 4b28d1988_slices_1-2-3-4.zip
mAafd9591fe mutant 1 57A afd9591fe_slices_1-x-2-3.zip
mAafd9591fe mutant 2 55B afd9591fe_slices_1-x-2-3.zip
mAafd9591fe mutant 3 52B afd9591fe_slices_1-x-2-3.zip
mAca6c69ea5 mutant 1 31A ca6c69ea5_slices_x-1-2-3.zip
mAca6c69ea5 mutant 2 46A ca6c69ea5_slices_x-1-2-3.zip
mAca6c69ea5 mutant 3 54B ca6c69ea5_slices_x-1-2-3.zip
mA96d0a15df mutant 1 45A 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 2 52A 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 3 15B 96d0a15df_slices_1-2-3-4.zip
mA96d0a15df mutant 4 54B 96d0a15df_slices_1-2-3-4.zip
mAcebfc8537 mutant 1 37A cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 2 62A cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 3 46B cebfc8537_slices_1-2-3-4.zip
mAcebfc8537 mutant 4 41B cebfc8537_slices_1-2-3-4.zip
wA8d77d5ac7 WT 1 87A 8d77d5ac7_slices_1-2-x-x.zip
wA8d77d5ac7 WT 2 42A 8d77d5ac7_slices_1-2-x-x.zip
wA5b0d51463 WT 1 87A 5b0d51463_slices_1-x-x-2.zip
wA5b0d51463 WT 2 62B 5b0d51463_slices_1-x-x-2.zip
wA64091321c WT 1 61A 64091321c_slices_1-x-2-x.zip
wA64091321c WT 2 43B 64091321c_slices_1-x-2-x.zip
wA411d5e724 WT 1 56A 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 2 52A 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 3 58B 411d5e724_slices_1-2-3-4.zip
wA411d5e724 WT 4 41B 411d5e724_slices_1-2-3-4.zip
wA6c2ae5fac WT 1 12A 6c2ae5fac_slices_1-2-3-x.zip
wA6c2ae5fac WT 2 54A 6c2ae5fac_slices_1-2-3-x.zip
wA6c2ae5fac WT 3 56B 6c2ae5fac_slices_1-2-3-x.zip

Data Files

File NameSizeChecksum (SHA256)
77156b7c6_slices_1-2-3.zip3895243167dbfac5dc3495cf1ef7e668c0e5e3b3a13f7a4ca9990b9fe1da5bd955df54d4
afd9591fe_slices_1-x-2-3.zip503401055f33b4579b4a3e3bc794652c0c884af0b46e7d0077623b5ec6d4abaef3ee8c1e
96d0a15df_slices_1-2-3-4.zip39907316560667163f7ba70d1c5770e97e5248d88a8333924b5eeeffe944748679bf70cb
8d77d5ac7_slices_1-2-x-x.zip288818841c20f6b77e49cbff3aceed111f0c0159dba78d0bcda058703db56018bae3b7c5
cda0b7e8d_slices_1-2-3.zip297244779be21108d1542bcf7a7dc9a888e25b3b14b817502c6121f7fb6ebfcff8403852
ef53ba2e4_slices_1-2-3.zip306052598428f31eb43439f85b0d721ba4a46dcdf8050e8364930b14d1f58319f8ef2e55
77e145992_slices_1-2-3.zip31367403614e0a52fb4b84101e67072db22086208854d155d5abdb72f528be9e12004c39
6c2ae5fac_slices_1-2-3-x.zip3615351608a35dc539f868d3fac3f694b55266388b68a79581ff20143784ccd4907cba26
cebfc8537_slices_1-2-3-4.zip433828566404a7eb9ff228001d1e258187ab0b13dc5eedbf83df1c8844fee309b73e0342
d6969adb7_slices_1-2-3.zip3168904528cc08a08a7b8ffb1b41e77792db6c2a12d58be22cf5b476df9761481b7630bb
8bd30e9ba_slices_1-2-3.zip301925484cb8fc9ee0bae77bc984910653fac07f83aaa919adf9bb0d9b033f0d1f11169b
4b28d1988_slices_1-2-3-4.zip31882588a4996e1e394715e83d925fcc9c16327237e6103361636b39fc4dc3e0a69e5f2e
64091321c_slices_1-x-2-x.zip3161645564ea5e6d49d08e8dde8aa7b653bd3db84a9178efc3b757ad2abe5592fd9d0757
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