G2C::Electrophysiology

NR2AdC+NR2BdC knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices, with only one variable, LTP based on amplitude, significantly decreased in these mutant slices. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the NR2AdC+NR2BdC knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 16 (4) 3100 (150) 17 (4) 3090 (170) 0.43 0.98
PPF at 50ms, ampl ratio % 16 (4) 168.1 (4.4) 17 (4) 163.2 (4.4) 0.051 0.59
Burst 1, PPF at 10ms, ampl ratio % 16 (4) 151.2 (8.1) 17 (4) 132.2 (5.8) 6e-04 *** 0.32
Burst 1, EPSP3 depr, ampl ratio % 16 (4) 67.6 (2.5) 17 (4) 66.3 (3.2) 0.032 * 0.83
Burst 1, ave amplitude µV 16 (4) 1401 (64) 17 (4) 1507 (86) 0.054 0.51
Burst 2, ave amplitude µV 16 (4) 2250 (130) 17 (4) 2370 (130) 0.35 0.55
Burst 3, ave amplitude µV 16 (4) 2410 (130) 17 (4) 2450 (140) 0.49 0.84
Burst 4, ave amplitude µV 16 (4) 2400 (130) 17 (4) 2440 (130) 0.66 0.8
Burst 5, ave amplitude µV 16 (4) 2350 (120) 17 (4) 2390 (130) 0.75 0.78
Burst 6, ave amplitude µV 16 (4) 2280 (110) 17 (4) 2300 (130) 0.8 0.86
Burst 7, ave amplitude µV 16 (4) 2200 (110) 17 (4) 2200 (130) 0.81 0.98
Burst 8, ave amplitude µV 16 (4) 2150 (110) 17 (4) 2120 (120) 0.86 0.8
Burst 9, ave amplitude µV 16 (4) 2090 (100) 17 (4) 2040 (120) 0.86 0.65
Burst 10, ave amplitude µV 16 (4) 2060 (100) 17 (4) 1960 (120) 0.87 0.39
Burst 2-4 ave amplitude vs Burst 1 % 16 (4) 167.8 (5) 17 (4) 161.7 (5.7) 0.0075 ** 0.64
Burst 8-10 ave amplitude vs Burst 2-4 % 16 (4) 89.3 (1.4) 17 (4) 83.9 (1.9) 0.29 0.092
LTP vs PTP, amplitude ratio % 16 (4) 81.6 (1.9) 17 (4) 81.3 (1.7) 0.32 0.92
LTP based on amplitude % 16 (4) 163.6 (4.2) 17 (4) 111.2 (1.9) 0.17 0.00013 ***

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 16 (4) 3100 (150) 17 (4) 3090 (170) 0.43 0.98

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 16 (4) 168.1 (4.4) 17 (4) 163.2 (4.4) 0.051 0.59

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 16 (4) 151.2 (8.1) 17 (4) 132.2 (5.8) 6e-04 *** 0.32
Burst 1, EPSP3 depr, ampl ratio % 16 (4) 67.6 (2.5) 17 (4) 66.3 (3.2) 0.032 * 0.83
Burst 1, ave amplitude µV 16 (4) 1401 (64) 17 (4) 1507 (86) 0.054 0.51
Burst 2, ave amplitude µV 16 (4) 2250 (130) 17 (4) 2370 (130) 0.35 0.55
Burst 3, ave amplitude µV 16 (4) 2410 (130) 17 (4) 2450 (140) 0.49 0.84
Burst 4, ave amplitude µV 16 (4) 2400 (130) 17 (4) 2440 (130) 0.66 0.8
Burst 5, ave amplitude µV 16 (4) 2350 (120) 17 (4) 2390 (130) 0.75 0.78
Burst 6, ave amplitude µV 16 (4) 2280 (110) 17 (4) 2300 (130) 0.8 0.86
Burst 7, ave amplitude µV 16 (4) 2200 (110) 17 (4) 2200 (130) 0.81 0.98
Burst 8, ave amplitude µV 16 (4) 2150 (110) 17 (4) 2120 (120) 0.86 0.8
Burst 9, ave amplitude µV 16 (4) 2090 (100) 17 (4) 2040 (120) 0.86 0.65
Burst 10, ave amplitude µV 16 (4) 2060 (100) 17 (4) 1960 (120) 0.87 0.39
Burst 2-4 ave amplitude vs Burst 1 % 16 (4) 167.8 (5) 17 (4) 161.7 (5.7) 0.0075 ** 0.64
Burst 8-10 ave amplitude vs Burst 2-4 % 16 (4) 89.3 (1.4) 17 (4) 83.9 (1.9) 0.29 0.092

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 16 (4) 81.6 (1.9) 17 (4) 81.3 (1.7) 0.32 0.92
LTP based on amplitude % 16 (4) 163.6 (4.2) 17 (4) 111.2 (1.9) 0.17 0.00013 ***

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT4
mutant4

 

Individual Mice

Mouse IDGenotype
wB0a4c3c508WT
wBdcef7f195WT
wA80f389d62WT
wA52588b034WT
mB80f389d62mutant
mA37aeabba6mutant
mAdcef7f195mutant
mA0a4c3c508mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA0a4c3c508 mutant 1 61A 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 2 46B 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 3 52B 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 5 42A 0a4c3c508_slices_4-5-6-7.zip
mAdcef7f195 mutant 1 12A dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 2 44A dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 3 52B dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 4 46B dcef7f195_slices_1-2-3-4.zip
mB80f389d62 mutant 4 58A 80f389d62_slices_4-5-6-7.zip
mB80f389d62 mutant 5 42A 80f389d62_slices_4-5-6-7.zip
mB80f389d62 mutant 6 24B 80f389d62_slices_4-5-6-7.zip
mA37aeabba6 mutant 1 55A 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 2 61A 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 3 46B 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 4 38A 37aeabba6_slices_4-5-6-x.zip
mA37aeabba6 mutant 5 54A 37aeabba6_slices_4-5-6-x.zip
mA37aeabba6 mutant 6 46B 37aeabba6_slices_4-5-6-x.zip
wB0a4c3c508 WT 4 44A 0a4c3c508_slices_4-5-6-7.zip
wB0a4c3c508 WT 6 56B 0a4c3c508_slices_4-5-6-7.zip
wB0a4c3c508 WT 7 84B 0a4c3c508_slices_4-5-6-7.zip
wBdcef7f195 WT 5 47A dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 6 53A dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 7 38B dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 8 84B dcef7f195_slices_5-6-7-8.zip
wA80f389d62 WT 1 46A 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 2 43A 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 3 46B 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 7 52B 80f389d62_slices_4-5-6-7.zip
wA52588b034 WT 1 55A 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 2 61A 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 3 55B 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 4 58A 52588b034_slices_4-5-x-x.zip
wA52588b034 WT 5 62A 52588b034_slices_4-5-x-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA0a4c3c508 mutant 1 61A 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 2 46B 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 3 52B 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 5 42A 0a4c3c508_slices_4-5-6-7.zip
mAdcef7f195 mutant 1 12A dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 2 44A dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 3 52B dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 4 46B dcef7f195_slices_1-2-3-4.zip
mB80f389d62 mutant 4 58A 80f389d62_slices_4-5-6-7.zip
mB80f389d62 mutant 5 42A 80f389d62_slices_4-5-6-7.zip
mB80f389d62 mutant 6 24B 80f389d62_slices_4-5-6-7.zip
mA37aeabba6 mutant 1 55A 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 2 61A 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 3 46B 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 4 38A 37aeabba6_slices_4-5-6-x.zip
mA37aeabba6 mutant 5 54A 37aeabba6_slices_4-5-6-x.zip
mA37aeabba6 mutant 6 46B 37aeabba6_slices_4-5-6-x.zip
wB0a4c3c508 WT 4 44A 0a4c3c508_slices_4-5-6-7.zip
wB0a4c3c508 WT 6 56B 0a4c3c508_slices_4-5-6-7.zip
wB0a4c3c508 WT 7 84B 0a4c3c508_slices_4-5-6-7.zip
wBdcef7f195 WT 5 47A dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 6 53A dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 7 38B dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 8 84B dcef7f195_slices_5-6-7-8.zip
wA80f389d62 WT 1 46A 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 2 43A 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 3 46B 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 7 52B 80f389d62_slices_4-5-6-7.zip
wA52588b034 WT 1 55A 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 2 61A 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 3 55B 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 4 58A 52588b034_slices_4-5-x-x.zip
wA52588b034 WT 5 62A 52588b034_slices_4-5-x-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA0a4c3c508 mutant 1 61A 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 2 46B 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 3 52B 0a4c3c508_slices_x-1-2-3.zip
mA0a4c3c508 mutant 5 42A 0a4c3c508_slices_4-5-6-7.zip
mAdcef7f195 mutant 1 12A dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 2 44A dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 3 52B dcef7f195_slices_1-2-3-4.zip
mAdcef7f195 mutant 4 46B dcef7f195_slices_1-2-3-4.zip
mB80f389d62 mutant 4 58A 80f389d62_slices_4-5-6-7.zip
mB80f389d62 mutant 5 42A 80f389d62_slices_4-5-6-7.zip
mB80f389d62 mutant 6 24B 80f389d62_slices_4-5-6-7.zip
mA37aeabba6 mutant 1 55A 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 2 61A 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 3 46B 37aeabba6_slices_1-2-3-x.zip
mA37aeabba6 mutant 4 38A 37aeabba6_slices_4-5-6-x.zip
mA37aeabba6 mutant 5 54A 37aeabba6_slices_4-5-6-x.zip
mA37aeabba6 mutant 6 46B 37aeabba6_slices_4-5-6-x.zip
wB0a4c3c508 WT 4 44A 0a4c3c508_slices_4-5-6-7.zip
wB0a4c3c508 WT 6 56B 0a4c3c508_slices_4-5-6-7.zip
wB0a4c3c508 WT 7 84B 0a4c3c508_slices_4-5-6-7.zip
wBdcef7f195 WT 5 47A dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 6 53A dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 7 38B dcef7f195_slices_5-6-7-8.zip
wBdcef7f195 WT 8 84B dcef7f195_slices_5-6-7-8.zip
wA80f389d62 WT 1 46A 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 2 43A 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 3 46B 80f389d62_slices_1-2-3-x.zip
wA80f389d62 WT 7 52B 80f389d62_slices_4-5-6-7.zip
wA52588b034 WT 1 55A 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 2 61A 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 3 55B 52588b034_slices_1-2-3-x.zip
wA52588b034 WT 4 58A 52588b034_slices_4-5-x-x.zip
wA52588b034 WT 5 62A 52588b034_slices_4-5-x-x.zip

Data Files

File NameSizeChecksum (SHA256)
80f389d62_slices_4-5-6-7.zip575985615f33c4cfc37d022d7571d8bb9b2e67ba59e8d314b4d42cb2189c33201dcf7eff
52588b034_slices_1-2-3-x.zip30366111cb5fcf4fce8e22f132d917228eab82ddd0f2b348f87a041c6ad7cd509bd54eff
0a4c3c508_slices_x-1-2-3.zip299260435226b85cb8d559cc47e1df081668eba03e5602eeea532aa6412173c58ee4c586
52588b034_slices_4-5-x-x.zip455824073f890654b7ccc310f11e8e25e9b75acb7a64cac87e89ede0ed796e02158c6f8b
37aeabba6_slices_1-2-3-x.zip28743948b982bf2d2ee281495cca50264b11c2a3fdf4b98a36d275507d9e1b4bd57f2efa
dcef7f195_slices_1-2-3-4.zip331498417e8c9e9755fa7397f8c34689617786afc85beea4b921e31694fbd338361f76c9
0a4c3c508_slices_4-5-6-7.zip50336288bbbc68e82323cca5ff0d15e8c8bf6c105645fe87a5a0e84610b7810ec844cf43
37aeabba6_slices_4-5-6-x.zip52979379f22c1d77051afc91787494a93a257f58a96f122b039fb6055e25fda0ba364ab1
80f389d62_slices_1-2-3-x.zip29453982ad6fb9afb665b03b25f447d6b25242eb23c498b296e942910bedbd34807fd555
dcef7f195_slices_5-6-7-8.zip43146610544e40da6cf0f93e34ebb4e09608a16ffa4b01cdf820c2bdbce0699a99e311a1
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