G2C::Electrophysiology

Iqsec3 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with no electrophysiological variables significantly affected by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Iqsec3 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3380 (180) 20 (5) 3060 (130) 0.15 0.26
PPF at 50ms, ampl ratio % 17 (5) 172.9 (4.4) 20 (5) 174.9 (3) 0.86 0.62
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 200.4 (7.2) 20 (5) 200.8 (6.8) 0.98 0.94
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 59.9 (2.3) 20 (5) 60.1 (2.5) 0.077 0.97
Burst 1, ave amplitude µV 17 (5) 1437 (64) 20 (5) 1330 (43) 0.15 0.28
Burst 2, ave amplitude µV 17 (5) 2200 (120) 20 (5) 1996 (88) 0.098 0.3
Burst 3, ave amplitude µV 17 (5) 2453 (127) 20 (5) 2239 (98) 0.094 0.32
Burst 4, ave amplitude µV 17 (5) 2530 (120) 20 (5) 2280 (100) 0.15 0.23
Burst 5, ave amplitude µV 17 (5) 2471 (118) 20 (5) 2232 (91) 0.22 0.2
Burst 6, ave amplitude µV 17 (5) 2395 (112) 20 (5) 2177 (86) 0.28 0.2
Burst 7, ave amplitude µV 17 (5) 2308 (113) 20 (5) 2096 (87) 0.27 0.21
Burst 8, ave amplitude µV 17 (5) 2246 (104) 20 (5) 2033 (89) 0.27 0.2
Burst 9, ave amplitude µV 17 (5) 2176 (107) 20 (5) 1982 (82) 0.29 0.23
Burst 10, ave amplitude µV 17 (5) 2132 (104) 20 (5) 1926 (81) 0.29 0.19
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 166.6 (4.1) 20 (5) 162.7 (4) 0.15 0.59
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 91.7 (1.5) 20 (5) 92 (1.5) 0.006 ** 0.92
LTP vs PTP, amplitude ratio % 17 (5) 83.6 (0.8) 20 (5) 83.2 (1) 0.23 0.81
LTP based on amplitude % 17 (5) 174.7 (4.5) 20 (5) 171.9 (6.1) 0.43 0.74

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3380 (180) 20 (5) 3060 (130) 0.15 0.26

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 17 (5) 172.9 (4.4) 20 (5) 174.9 (3) 0.86 0.62

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 200.4 (7.2) 20 (5) 200.8 (6.8) 0.98 0.94
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 59.9 (2.3) 20 (5) 60.1 (2.5) 0.077 0.97
Burst 1, ave amplitude µV 17 (5) 1437 (64) 20 (5) 1330 (43) 0.15 0.28
Burst 2, ave amplitude µV 17 (5) 2200 (120) 20 (5) 1996 (88) 0.098 0.3
Burst 3, ave amplitude µV 17 (5) 2453 (127) 20 (5) 2239 (98) 0.094 0.32
Burst 4, ave amplitude µV 17 (5) 2530 (120) 20 (5) 2280 (100) 0.15 0.23
Burst 5, ave amplitude µV 17 (5) 2471 (118) 20 (5) 2232 (91) 0.22 0.2
Burst 6, ave amplitude µV 17 (5) 2395 (112) 20 (5) 2177 (86) 0.28 0.2
Burst 7, ave amplitude µV 17 (5) 2308 (113) 20 (5) 2096 (87) 0.27 0.21
Burst 8, ave amplitude µV 17 (5) 2246 (104) 20 (5) 2033 (89) 0.27 0.2
Burst 9, ave amplitude µV 17 (5) 2176 (107) 20 (5) 1982 (82) 0.29 0.23
Burst 10, ave amplitude µV 17 (5) 2132 (104) 20 (5) 1926 (81) 0.29 0.19
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 166.6 (4.1) 20 (5) 162.7 (4) 0.15 0.59
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 91.7 (1.5) 20 (5) 92 (1.5) 0.006 ** 0.92

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 17 (5) 83.6 (0.8) 20 (5) 83.2 (1) 0.23 0.81
LTP based on amplitude % 17 (5) 174.7 (4.5) 20 (5) 171.9 (6.1) 0.43 0.74

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wB924a7fdf6WT
wAaf92cfe12WT
wBae1ce8cbcWT
wA0d75e8d09WT
wB95c94856aWT
mA95c94856amutant
mA924a7fdf6mutant
mBaf92cfe12mutant
mB0d75e8d09mutant
mAae1ce8cbcmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA924a7fdf6 mutant 1 12A 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 2 52A 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 3 51B 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 4 12B 924a7fdf6_slices_1-2-3-4.zip
mAae1ce8cbc mutant 1 12A ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 2 61A ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 3 61B ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 4 46B ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 5 12A ae1ce8cbc_slices_5-6-7-8.zip
mB0d75e8d09 mutant 3 41B 0d75e8d09_slices_1-2-3-4.zip
mB0d75e8d09 mutant 5 46A 0d75e8d09_slices_5-6-7-x.zip
mB0d75e8d09 mutant 6 61A 0d75e8d09_slices_5-6-7-x.zip
mB0d75e8d09 mutant 7 46B 0d75e8d09_slices_5-6-7-x.zip
mBaf92cfe12 mutant 5 67A af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 6 54A af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 7 46B af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 8 61B af92cfe12_slices_5-6-7-8.zip
mA95c94856a mutant 1 46A 95c94856a_slices_1-2-3-4.zip
mA95c94856a mutant 2 61A 95c94856a_slices_1-2-3-4.zip
mA95c94856a mutant 3 62B 95c94856a_slices_1-2-3-4.zip
wB924a7fdf6 WT 5 46A 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 6 52A 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 7 46B 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 8 52B 924a7fdf6_slices_5-6-7-8.zip
wBae1ce8cbc WT 6 51A ae1ce8cbc_slices_5-6-7-8.zip
wBae1ce8cbc WT 7 56B ae1ce8cbc_slices_5-6-7-8.zip
wBae1ce8cbc WT 8 52B ae1ce8cbc_slices_5-6-7-8.zip
wA0d75e8d09 WT 1 58A 0d75e8d09_slices_1-2-3-4.zip
wA0d75e8d09 WT 2 52A 0d75e8d09_slices_1-2-3-4.zip
wA0d75e8d09 WT 4 15B 0d75e8d09_slices_1-2-3-4.zip
wAaf92cfe12 WT 1 56A af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 2 52A af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 3 84B af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 4 55B af92cfe12_slices_1-2-3-4.zip
wB95c94856a WT 4 15B 95c94856a_slices_1-2-3-4.zip
wB95c94856a WT 6 61A 95c94856a_slices_5-6-7-x.zip
wB95c94856a WT 7 58B 95c94856a_slices_5-6-7-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA924a7fdf6 mutant 1 12A 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 2 52A 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 3 51B 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 4 12B 924a7fdf6_slices_1-2-3-4.zip
mAae1ce8cbc mutant 1 12A ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 2 61A ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 3 61B ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 4 46B ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 5 12A ae1ce8cbc_slices_5-6-7-8.zip
mB0d75e8d09 mutant 3 41B 0d75e8d09_slices_1-2-3-4.zip
mB0d75e8d09 mutant 5 46A 0d75e8d09_slices_5-6-7-x.zip
mB0d75e8d09 mutant 6 61A 0d75e8d09_slices_5-6-7-x.zip
mB0d75e8d09 mutant 7 46B 0d75e8d09_slices_5-6-7-x.zip
mBaf92cfe12 mutant 5 67A af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 6 54A af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 7 46B af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 8 61B af92cfe12_slices_5-6-7-8.zip
mA95c94856a mutant 1 46A 95c94856a_slices_1-2-3-4.zip
mA95c94856a mutant 2 61A 95c94856a_slices_1-2-3-4.zip
mA95c94856a mutant 3 62B 95c94856a_slices_1-2-3-4.zip
wB924a7fdf6 WT 5 46A 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 6 52A 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 7 46B 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 8 52B 924a7fdf6_slices_5-6-7-8.zip
wBae1ce8cbc WT 6 51A ae1ce8cbc_slices_5-6-7-8.zip
wBae1ce8cbc WT 7 56B ae1ce8cbc_slices_5-6-7-8.zip
wBae1ce8cbc WT 8 52B ae1ce8cbc_slices_5-6-7-8.zip
wA0d75e8d09 WT 1 58A 0d75e8d09_slices_1-2-3-4.zip
wA0d75e8d09 WT 2 52A 0d75e8d09_slices_1-2-3-4.zip
wA0d75e8d09 WT 4 15B 0d75e8d09_slices_1-2-3-4.zip
wAaf92cfe12 WT 1 56A af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 2 52A af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 3 84B af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 4 55B af92cfe12_slices_1-2-3-4.zip
wB95c94856a WT 4 15B 95c94856a_slices_1-2-3-4.zip
wB95c94856a WT 6 61A 95c94856a_slices_5-6-7-x.zip
wB95c94856a WT 7 58B 95c94856a_slices_5-6-7-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA924a7fdf6 mutant 1 12A 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 2 52A 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 3 51B 924a7fdf6_slices_1-2-3-4.zip
mA924a7fdf6 mutant 4 12B 924a7fdf6_slices_1-2-3-4.zip
mAae1ce8cbc mutant 1 12A ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 2 61A ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 3 61B ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 4 46B ae1ce8cbc_slices_1-2-3-4.zip
mAae1ce8cbc mutant 5 12A ae1ce8cbc_slices_5-6-7-8.zip
mB0d75e8d09 mutant 3 41B 0d75e8d09_slices_1-2-3-4.zip
mB0d75e8d09 mutant 5 46A 0d75e8d09_slices_5-6-7-x.zip
mB0d75e8d09 mutant 6 61A 0d75e8d09_slices_5-6-7-x.zip
mB0d75e8d09 mutant 7 46B 0d75e8d09_slices_5-6-7-x.zip
mBaf92cfe12 mutant 5 67A af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 6 54A af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 7 46B af92cfe12_slices_5-6-7-8.zip
mBaf92cfe12 mutant 8 61B af92cfe12_slices_5-6-7-8.zip
mA95c94856a mutant 1 46A 95c94856a_slices_1-2-3-4.zip
mA95c94856a mutant 2 61A 95c94856a_slices_1-2-3-4.zip
mA95c94856a mutant 3 62B 95c94856a_slices_1-2-3-4.zip
wB924a7fdf6 WT 5 46A 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 6 52A 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 7 46B 924a7fdf6_slices_5-6-7-8.zip
wB924a7fdf6 WT 8 52B 924a7fdf6_slices_5-6-7-8.zip
wBae1ce8cbc WT 6 51A ae1ce8cbc_slices_5-6-7-8.zip
wBae1ce8cbc WT 7 56B ae1ce8cbc_slices_5-6-7-8.zip
wBae1ce8cbc WT 8 52B ae1ce8cbc_slices_5-6-7-8.zip
wA0d75e8d09 WT 1 58A 0d75e8d09_slices_1-2-3-4.zip
wA0d75e8d09 WT 2 52A 0d75e8d09_slices_1-2-3-4.zip
wA0d75e8d09 WT 4 15B 0d75e8d09_slices_1-2-3-4.zip
wAaf92cfe12 WT 1 56A af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 2 52A af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 3 84B af92cfe12_slices_1-2-3-4.zip
wAaf92cfe12 WT 4 55B af92cfe12_slices_1-2-3-4.zip
wB95c94856a WT 4 15B 95c94856a_slices_1-2-3-4.zip
wB95c94856a WT 6 61A 95c94856a_slices_5-6-7-x.zip
wB95c94856a WT 7 58B 95c94856a_slices_5-6-7-x.zip

Data Files

File NameSizeChecksum (SHA256)
0d75e8d09_slices_5-6-7-x.zip445900492f55feca7354229bfc7f09e63aa82cb65fc24b9afdbc56eb3fb2b149d7dd4e08
924a7fdf6_slices_1-2-3-4.zip37109388723f3441ef20d65f67a568e1eee38990ed563145c1d6ef345b323c5d800db652
95c94856a_slices_5-6-7-x.zip46678328ca8721612e1a430de32d9c45485da216ba3c7f5452a4edf5a6eb76893169d4df
0d75e8d09_slices_1-2-3-4.zip3437034145eec41676e5eb410f6f051dc8c4f00ccccbcd5040853b2769417e1e6b1f8131
ae1ce8cbc_slices_1-2-3-4.zip34657115b0190e3304aba0ea0b88a2c4e2172af9bc4104c08663e8fccc7eeecdf94acf3d
af92cfe12_slices_1-2-3-4.zip34348847a4c3c7cfe4ab9e7e07649a770b11162723d992baeb506d849301279c22f9e1f6
af92cfe12_slices_5-6-7-8.zip50304692a88635a1ef9f9a9f5e7b58ad6d947710c267402864571da195af6664b434a1be
95c94856a_slices_1-2-3-4.zip35229585ba00e6cf8b838819b36d4ec46b95d45396227090ce7d43e4daf74c82ab56d0ea
924a7fdf6_slices_5-6-7-8.zip47364465143aac0562f519365cadf6ea7cb4fa99019459e25399dcc79933f99e1a7f5cdc
ae1ce8cbc_slices_5-6-7-8.zip43857991cc3a00d23160c81e79f14ad3d995ba8cc07038a6d81534f15a28acd9f860f999
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