G2C::Electrophysiology

PIKE knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices, with only one variable, LTP vs PTP amplitude ratio, significantly increased in these mutants. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the PIKE knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 21 (7) 2980 (140) 22 (7) 3180 (130) 0.051 0.43
PPF at 50ms, ampl ratio % 21 (7) 184.7 (4.7) 22 (7) 176.6 (4.1) 0.076 0.33
Burst 1, PPF at 10ms, ampl ratio % 21 (7) 212.6 (8.1) 22 (7) 198.7 (6.8) 0.035 * 0.35
Burst 1, EPSP3 depr, ampl ratio % 21 (7) 66.4 (3) 22 (7) 66.8 (2.1) 0.018 * 0.94
Burst 1, ave amplitude µV 21 (7) 1228 (38) 22 (7) 1420 (53) 0.13 0.039 *
Burst 2, ave amplitude µV 21 (7) 1899 (89) 22 (7) 2107 (94) 0.11 0.23
Burst 3, ave amplitude µV 21 (7) 2220 (110) 22 (7) 2440 (120) 0.15 0.29
Burst 4, ave amplitude µV 21 (7) 2330 (99) 22 (7) 2589 (119) 0.19 0.18
Burst 5, ave amplitude µV 21 (7) 2322 (91) 22 (7) 2602 (116) 0.23 0.13
Burst 6, ave amplitude µV 21 (7) 2277 (89) 22 (7) 2568 (112) 0.21 0.11
Burst 7, ave amplitude µV 21 (7) 2213 (86) 22 (7) 2516 (108) 0.2 0.091
Burst 8, ave amplitude µV 21 (7) 2145 (84) 22 (7) 2443 (106) 0.16 0.098
Burst 9, ave amplitude µV 21 (7) 2088 (88) 22 (7) 2392 (108) 0.17 0.096
Burst 10, ave amplitude µV 21 (7) 2036 (89) 22 (7) 2335 (106) 0.17 0.1
Burst 2-4 ave amplitude vs Burst 1 % 21 (7) 177.1 (6.1) 22 (7) 166.6 (4.7) 0.25 0.25
Burst 8-10 ave amplitude vs Burst 2-4 % 21 (7) 97.5 (1.5) 22 (7) 100.5 (1.2) 0.0018 ** 0.33
LTP vs PTP, amplitude ratio % 21 (7) 77.8 (1.4) 22 (7) 88.5 (1.7) 0.95 1.6e-05 ***
LTP based on amplitude % 21 (7) 179.7 (8.4) 22 (7) 178 (5) 0.024 * 0.9

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 21 (7) 2980 (140) 22 (7) 3180 (130) 0.051 0.43

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 21 (7) 184.7 (4.7) 22 (7) 176.6 (4.1) 0.076 0.33

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 21 (7) 212.6 (8.1) 22 (7) 198.7 (6.8) 0.035 * 0.35
Burst 1, EPSP3 depr, ampl ratio % 21 (7) 66.4 (3) 22 (7) 66.8 (2.1) 0.018 * 0.94
Burst 1, ave amplitude µV 21 (7) 1228 (38) 22 (7) 1420 (53) 0.13 0.039 *
Burst 2, ave amplitude µV 21 (7) 1899 (89) 22 (7) 2107 (94) 0.11 0.23
Burst 3, ave amplitude µV 21 (7) 2220 (110) 22 (7) 2440 (120) 0.15 0.29
Burst 4, ave amplitude µV 21 (7) 2330 (99) 22 (7) 2589 (119) 0.19 0.18
Burst 5, ave amplitude µV 21 (7) 2322 (91) 22 (7) 2602 (116) 0.23 0.13
Burst 6, ave amplitude µV 21 (7) 2277 (89) 22 (7) 2568 (112) 0.21 0.11
Burst 7, ave amplitude µV 21 (7) 2213 (86) 22 (7) 2516 (108) 0.2 0.091
Burst 8, ave amplitude µV 21 (7) 2145 (84) 22 (7) 2443 (106) 0.16 0.098
Burst 9, ave amplitude µV 21 (7) 2088 (88) 22 (7) 2392 (108) 0.17 0.096
Burst 10, ave amplitude µV 21 (7) 2036 (89) 22 (7) 2335 (106) 0.17 0.1
Burst 2-4 ave amplitude vs Burst 1 % 21 (7) 177.1 (6.1) 22 (7) 166.6 (4.7) 0.25 0.25
Burst 8-10 ave amplitude vs Burst 2-4 % 21 (7) 97.5 (1.5) 22 (7) 100.5 (1.2) 0.0018 ** 0.33

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 21 (7) 77.8 (1.4) 22 (7) 88.5 (1.7) 0.95 1.6e-05 ***
LTP based on amplitude % 21 (7) 179.7 (8.4) 22 (7) 178 (5) 0.024 * 0.9

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT7
mutant8

 

Individual Mice

Mouse IDGenotype
wAa58d993bcWT
wB9905436eaWT
wBa124281f1WT
wB80b60e33dWT
wAd022126dfWT
wA9aa6e5d15WT
wB9aa6e5d15WT
mA12689cf33mutant
mA7ffee1cc5mutant
mA1d8c77d6cmutant
mB30d57eb92mutant
mB7ffee1cc5mutant
mA9905436eamutant
mA30d57eb92mutant
mAa124281f1mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA7ffee1cc5 mutant 1 46A 7ffee1cc5_slices_1-2-x-3.zip
mA7ffee1cc5 mutant 2 52A 7ffee1cc5_slices_1-2-x-3.zip
mA7ffee1cc5 mutant 3 53B 7ffee1cc5_slices_1-2-x-3.zip
mB7ffee1cc5 mutant 4 55A 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 5 62A 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 6 37B 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 7 62B 7ffee1cc5_slices_4-5-6-7.zip
mA9905436ea mutant 1 46B 9905436ea_slices_x-x-1-x.zip
mA30d57eb92 mutant 2 12B 30d57eb92_slices_1-x-2-3.zip
mB30d57eb92 mutant 4 12A 30d57eb92_slices_4-x-5-6.zip
mB30d57eb92 mutant 5 58B 30d57eb92_slices_4-x-5-6.zip
mB30d57eb92 mutant 6 42B 30d57eb92_slices_4-x-5-6.zip
mA1d8c77d6c mutant 1 46A 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 2 52A 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 3 12B 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 4 62B 1d8c77d6c_slices_1-2-3-4.zip
mAa124281f1 mutant 1 15A a124281f1_slices_1-2-3-4.zip
mAa124281f1 mutant 2 61A a124281f1_slices_1-2-3-4.zip
mAa124281f1 mutant 4 52B a124281f1_slices_1-2-3-4.zip
mA12689cf33 mutant 1 12A 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 2 52A 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 3 46B 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 4 61B 12689cf33_slices_1-2-3-4.zip
wAd022126df WT 1 46A d022126df_slices_1-2-x-x.zip
wAd022126df WT 2 61A d022126df_slices_1-2-x-x.zip
wA9aa6e5d15 WT 1 46A 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 2 62A 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 3 46B 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 4 52B 9aa6e5d15_slices_1-2-3-4.zip
wB9aa6e5d15 WT 5 12A 9aa6e5d15_slices_5-6-7-x.zip
wB9aa6e5d15 WT 6 52A 9aa6e5d15_slices_5-6-7-x.zip
wB9aa6e5d15 WT 7 15B 9aa6e5d15_slices_5-6-7-x.zip
wB9905436ea WT 4 42B 9905436ea_slices_x-2-3-4.zip
wB80b60e33d WT 5 58A 80b60e33d_slices_5-x-x-6.zip
wB80b60e33d WT 6 61B 80b60e33d_slices_5-x-x-6.zip
wAa58d993bc WT 1 15A a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 2 87A a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 3 55B a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 4 42B a58d993bc_slices_1-2-3-4.zip
wBa124281f1 WT 3 46B a124281f1_slices_1-2-3-4.zip
wBa124281f1 WT 5 58A a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 6 61A a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 7 12B a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 8 87B a124281f1_slices_5-6-7-8.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA7ffee1cc5 mutant 1 46A 7ffee1cc5_slices_1-2-x-3.zip
mA7ffee1cc5 mutant 2 52A 7ffee1cc5_slices_1-2-x-3.zip
mA7ffee1cc5 mutant 3 53B 7ffee1cc5_slices_1-2-x-3.zip
mB7ffee1cc5 mutant 4 55A 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 5 62A 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 6 37B 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 7 62B 7ffee1cc5_slices_4-5-6-7.zip
mA9905436ea mutant 1 46B 9905436ea_slices_x-x-1-x.zip
mA30d57eb92 mutant 2 12B 30d57eb92_slices_1-x-2-3.zip
mB30d57eb92 mutant 4 12A 30d57eb92_slices_4-x-5-6.zip
mB30d57eb92 mutant 5 58B 30d57eb92_slices_4-x-5-6.zip
mB30d57eb92 mutant 6 42B 30d57eb92_slices_4-x-5-6.zip
mA1d8c77d6c mutant 1 46A 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 2 52A 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 3 12B 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 4 62B 1d8c77d6c_slices_1-2-3-4.zip
mAa124281f1 mutant 1 15A a124281f1_slices_1-2-3-4.zip
mAa124281f1 mutant 2 61A a124281f1_slices_1-2-3-4.zip
mAa124281f1 mutant 4 52B a124281f1_slices_1-2-3-4.zip
mA12689cf33 mutant 1 12A 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 2 52A 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 3 46B 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 4 61B 12689cf33_slices_1-2-3-4.zip
wAd022126df WT 1 46A d022126df_slices_1-2-x-x.zip
wAd022126df WT 2 61A d022126df_slices_1-2-x-x.zip
wA9aa6e5d15 WT 1 46A 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 2 62A 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 3 46B 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 4 52B 9aa6e5d15_slices_1-2-3-4.zip
wB9aa6e5d15 WT 5 12A 9aa6e5d15_slices_5-6-7-x.zip
wB9aa6e5d15 WT 6 52A 9aa6e5d15_slices_5-6-7-x.zip
wB9aa6e5d15 WT 7 15B 9aa6e5d15_slices_5-6-7-x.zip
wB9905436ea WT 4 42B 9905436ea_slices_x-2-3-4.zip
wB80b60e33d WT 5 58A 80b60e33d_slices_5-x-x-6.zip
wB80b60e33d WT 6 61B 80b60e33d_slices_5-x-x-6.zip
wAa58d993bc WT 1 15A a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 2 87A a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 3 55B a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 4 42B a58d993bc_slices_1-2-3-4.zip
wBa124281f1 WT 3 46B a124281f1_slices_1-2-3-4.zip
wBa124281f1 WT 5 58A a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 6 61A a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 7 12B a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 8 87B a124281f1_slices_5-6-7-8.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA7ffee1cc5 mutant 1 46A 7ffee1cc5_slices_1-2-x-3.zip
mA7ffee1cc5 mutant 2 52A 7ffee1cc5_slices_1-2-x-3.zip
mA7ffee1cc5 mutant 3 53B 7ffee1cc5_slices_1-2-x-3.zip
mB7ffee1cc5 mutant 4 55A 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 5 62A 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 6 37B 7ffee1cc5_slices_4-5-6-7.zip
mB7ffee1cc5 mutant 7 62B 7ffee1cc5_slices_4-5-6-7.zip
mA9905436ea mutant 1 46B 9905436ea_slices_x-x-1-x.zip
mA30d57eb92 mutant 2 12B 30d57eb92_slices_1-x-2-3.zip
mB30d57eb92 mutant 4 12A 30d57eb92_slices_4-x-5-6.zip
mB30d57eb92 mutant 5 58B 30d57eb92_slices_4-x-5-6.zip
mB30d57eb92 mutant 6 42B 30d57eb92_slices_4-x-5-6.zip
mA1d8c77d6c mutant 1 46A 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 2 52A 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 3 12B 1d8c77d6c_slices_1-2-3-4.zip
mA1d8c77d6c mutant 4 62B 1d8c77d6c_slices_1-2-3-4.zip
mAa124281f1 mutant 1 15A a124281f1_slices_1-2-3-4.zip
mAa124281f1 mutant 2 61A a124281f1_slices_1-2-3-4.zip
mAa124281f1 mutant 4 52B a124281f1_slices_1-2-3-4.zip
mA12689cf33 mutant 1 12A 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 2 52A 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 3 46B 12689cf33_slices_1-2-3-4.zip
mA12689cf33 mutant 4 61B 12689cf33_slices_1-2-3-4.zip
wAd022126df WT 1 46A d022126df_slices_1-2-x-x.zip
wAd022126df WT 2 61A d022126df_slices_1-2-x-x.zip
wA9aa6e5d15 WT 1 46A 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 2 62A 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 3 46B 9aa6e5d15_slices_1-2-3-4.zip
wA9aa6e5d15 WT 4 52B 9aa6e5d15_slices_1-2-3-4.zip
wB9aa6e5d15 WT 5 12A 9aa6e5d15_slices_5-6-7-x.zip
wB9aa6e5d15 WT 6 52A 9aa6e5d15_slices_5-6-7-x.zip
wB9aa6e5d15 WT 7 15B 9aa6e5d15_slices_5-6-7-x.zip
wB9905436ea WT 4 42B 9905436ea_slices_x-2-3-4.zip
wB80b60e33d WT 5 58A 80b60e33d_slices_5-x-x-6.zip
wB80b60e33d WT 6 61B 80b60e33d_slices_5-x-x-6.zip
wAa58d993bc WT 1 15A a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 2 87A a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 3 55B a58d993bc_slices_1-2-3-4.zip
wAa58d993bc WT 4 42B a58d993bc_slices_1-2-3-4.zip
wBa124281f1 WT 3 46B a124281f1_slices_1-2-3-4.zip
wBa124281f1 WT 5 58A a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 6 61A a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 7 12B a124281f1_slices_5-6-7-8.zip
wBa124281f1 WT 8 87B a124281f1_slices_5-6-7-8.zip

Data Files

File NameSizeChecksum (SHA256)
1d8c77d6c_slices_1-2-3-4.zip34628642c48f069314bfa3ad73d8ebebbc1fee0009277b639c006f9c607194ea50651586
12689cf33_slices_1-2-3-4.zip3378945321b80623e4f3a5ac4d5b31a89e96c5760a60b44b67c127681faded5e68b8ff79
d022126df_slices_1-2-x-x.zip351778971bd948f07e6bfa52a792df7b5b85d91038961fe2c947340335602d2ef7bb9ab2
7ffee1cc5_slices_1-2-x-3.zip307554701a7c0106018dcf2f627331c1de4aae609546ca04600784461814135b81c436e8
a124281f1_slices_5-6-7-8.zip4792669404a398b2bf7568b5e4a8cc8f542c082dff2786bddd132eb0d8c882c9f859f165
80b60e33d_slices_5-x-x-6.zip41602334bc9503e80fe61c0d72664b662000ce187e345f49553cd32bf06e24ed57b00cbb
7ffee1cc5_slices_4-5-6-7.zip46560706b9c37b02b4000708a643feddac9df502d66cded60be3a9534d5ad0577d082212
9905436ea_slices_x-2-3-4.zip390281548b59400bc018b0a59f3cac5a6dc500d638abd8964dabc8ec504bf59a10a230cf
9905436ea_slices_x-x-1-x.zip26121788388d712853b456e1ade4d109dea65ccbbf18cf1e1ca9661443d1895176bab83c
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EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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