G2C::Electrophysiology

Dusp7 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with no electrophysiological variables significantly affected by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

Enlarge this image (600 x 700)

The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Dusp7 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 14 (5) 2610 (130) 19 (6) 2940 (200) 0.091 0.33
PPF at 50ms, ampl ratio % 14 (5) 171.9 (6.1) 19 (6) 168 (5.2) 0.0037 ** 0.76
Burst 1, PPF at 10ms, ampl ratio % 14 (5) 190.9 (10.4) 19 (6) 163.7 (8.1) 0.024 * 0.17
Burst 1, EPSP3 depr, ampl ratio % 14 (5) 71.6 (4.9) 19 (6) 82.5 (4.4) 0.12 0.22
Burst 1, ave amplitude µV 14 (5) 1178 (83) 19 (6) 1152 (68) 0.036 * 0.87
Burst 2, ave amplitude µV 14 (5) 1790 (140) 19 (6) 1820 (130) 0.011 * 0.91
Burst 3, ave amplitude µV 14 (5) 2030 (170) 19 (6) 2090 (160) 0.028 * 0.85
Burst 4, ave amplitude µV 14 (5) 2130 (190) 19 (6) 2160 (170) 0.034 * 0.93
Burst 5, ave amplitude µV 14 (5) 2120 (200) 19 (6) 2140 (180) 0.055 0.96
Burst 6, ave amplitude µV 14 (5) 2070 (200) 19 (6) 2080 (180) 0.084 0.97
Burst 7, ave amplitude µV 14 (5) 1990 (190) 19 (6) 1980 (180) 0.09 0.97
Burst 8, ave amplitude µV 14 (5) 1920 (190) 19 (6) 1910 (180) 0.09 0.98
Burst 9, ave amplitude µV 14 (5) 1860 (190) 19 (6) 1850 (170) 0.09 0.97
Burst 10, ave amplitude µV 14 (5) 1800 (180) 19 (6) 1820 (170) 0.11 0.97
Burst 2-4 ave amplitude vs Burst 1 % 14 (5) 167.9 (5.2) 19 (6) 175 (6.1) 0.14 0.5
Burst 8-10 ave amplitude vs Burst 2-4 % 14 (5) 92.7 (3) 19 (6) 90.2 (2.7) 0.34 0.58
LTP vs PTP, amplitude ratio % 14 (5) 80.7 (1.3) 19 (6) 80.7 (1.1) 0.28 0.98
LTP based on amplitude % 14 (5) 169.8 (9.7) 19 (6) 173.6 (6.8) 0.4 0.76

 

Basal Synaptic Transmission

Enlarge this image (800 x 600)

Enlarge this image (640 x 480)

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 14 (5) 2610 (130) 19 (6) 2940 (200) 0.091 0.33

 

Paired Pulse Facilitation

Enlarge this image (800 x 600)

Enlarge this image (640 x 480)

 

Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 14 (5) 171.9 (6.1) 19 (6) 168 (5.2) 0.0037 ** 0.76

 

Theta Burst Stimulation

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

Enlarge this image (800 x 600)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

 

Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 14 (5) 190.9 (10.4) 19 (6) 163.7 (8.1) 0.024 * 0.17
Burst 1, EPSP3 depr, ampl ratio % 14 (5) 71.6 (4.9) 19 (6) 82.5 (4.4) 0.12 0.22
Burst 1, ave amplitude µV 14 (5) 1178 (83) 19 (6) 1152 (68) 0.036 * 0.87
Burst 2, ave amplitude µV 14 (5) 1790 (140) 19 (6) 1820 (130) 0.011 * 0.91
Burst 3, ave amplitude µV 14 (5) 2030 (170) 19 (6) 2090 (160) 0.028 * 0.85
Burst 4, ave amplitude µV 14 (5) 2130 (190) 19 (6) 2160 (170) 0.034 * 0.93
Burst 5, ave amplitude µV 14 (5) 2120 (200) 19 (6) 2140 (180) 0.055 0.96
Burst 6, ave amplitude µV 14 (5) 2070 (200) 19 (6) 2080 (180) 0.084 0.97
Burst 7, ave amplitude µV 14 (5) 1990 (190) 19 (6) 1980 (180) 0.09 0.97
Burst 8, ave amplitude µV 14 (5) 1920 (190) 19 (6) 1910 (180) 0.09 0.98
Burst 9, ave amplitude µV 14 (5) 1860 (190) 19 (6) 1850 (170) 0.09 0.97
Burst 10, ave amplitude µV 14 (5) 1800 (180) 19 (6) 1820 (170) 0.11 0.97
Burst 2-4 ave amplitude vs Burst 1 % 14 (5) 167.9 (5.2) 19 (6) 175 (6.1) 0.14 0.5
Burst 8-10 ave amplitude vs Burst 2-4 % 14 (5) 92.7 (3) 19 (6) 90.2 (2.7) 0.34 0.58

 

Long Term Potentiation

Enlarge this image (800 x 600)

Enlarge this image (640 x 480)

Enlarge this image (640 x 480)

 

Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 14 (5) 80.7 (1.3) 19 (6) 80.7 (1.1) 0.28 0.98
LTP based on amplitude % 14 (5) 169.8 (9.7) 19 (6) 173.6 (6.8) 0.4 0.76

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant6

 

Individual Mice

Mouse IDGenotype
wA7f1df342eWT
wAe6cb830a1WT
wAde5764030WT
wA7ad2ce46dWT
wA26c6708c8WT
mA5fbe48d4amutant
mAb9db07673mutant
mAd7d01f992mutant
mAa3eb6199bmutant
mAae169fe20mutant
mA665387fbamutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAb9db07673 mutant 1 46A b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 2 61A b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 3 55B b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 4 52B b9db07673_slices_1-2-3-4.zip
mAd7d01f992 mutant 3 15B d7d01f992_slices_1-2-3-4.zip
mAd7d01f992 mutant 4 41B d7d01f992_slices_1-2-3-4.zip
mAa3eb6199b mutant 1 53A a3eb6199b_slices_x-1-2-3.zip
mAa3eb6199b mutant 2 38B a3eb6199b_slices_x-1-2-3.zip
mAa3eb6199b mutant 3 61B a3eb6199b_slices_x-1-2-3.zip
mA665387fba mutant 2 62A 665387fba_slices_1-2-3-x.zip
mA665387fba mutant 3 38B 665387fba_slices_1-2-3-x.zip
mA5fbe48d4a mutant 1 55A 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 2 54A 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 3 46B 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 4 61B 5fbe48d4a_slices_1-2-3-4.zip
mAae169fe20 mutant 1 58A ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 2 62A ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 3 38B ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 4 62B ae169fe20_slices_1-2-3-4.zip
wA7ad2ce46d WT 1 58A 7ad2ce46d_slices_1-2-x-x.zip
wA7ad2ce46d WT 2 62A 7ad2ce46d_slices_1-2-x-x.zip
wAde5764030 WT 2 87B de5764030_slices_x-1-2-3.zip
wAde5764030 WT 3 62B de5764030_slices_x-1-2-3.zip
wA7f1df342e WT 1 38A 7f1df342e_slices_1-2-3-x.zip
wA7f1df342e WT 2 52A 7f1df342e_slices_1-2-3-x.zip
wA7f1df342e WT 3 58B 7f1df342e_slices_1-2-3-x.zip
wA26c6708c8 WT 1 12A 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 2 52A 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 3 45B 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 4 52B 26c6708c8_slices_1-2-3-4.zip
wAe6cb830a1 WT 1 43A e6cb830a1_slices_x-1-2-3.zip
wAe6cb830a1 WT 2 58B e6cb830a1_slices_x-1-2-3.zip
wAe6cb830a1 WT 3 41B e6cb830a1_slices_x-1-2-3.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAb9db07673 mutant 1 46A b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 2 61A b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 3 55B b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 4 52B b9db07673_slices_1-2-3-4.zip
mAd7d01f992 mutant 3 15B d7d01f992_slices_1-2-3-4.zip
mAd7d01f992 mutant 4 41B d7d01f992_slices_1-2-3-4.zip
mAa3eb6199b mutant 1 53A a3eb6199b_slices_x-1-2-3.zip
mAa3eb6199b mutant 2 38B a3eb6199b_slices_x-1-2-3.zip
mAa3eb6199b mutant 3 61B a3eb6199b_slices_x-1-2-3.zip
mA665387fba mutant 2 62A 665387fba_slices_1-2-3-x.zip
mA665387fba mutant 3 38B 665387fba_slices_1-2-3-x.zip
mA5fbe48d4a mutant 1 55A 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 2 54A 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 3 46B 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 4 61B 5fbe48d4a_slices_1-2-3-4.zip
mAae169fe20 mutant 1 58A ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 2 62A ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 3 38B ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 4 62B ae169fe20_slices_1-2-3-4.zip
wA7ad2ce46d WT 1 58A 7ad2ce46d_slices_1-2-x-x.zip
wA7ad2ce46d WT 2 62A 7ad2ce46d_slices_1-2-x-x.zip
wAde5764030 WT 2 87B de5764030_slices_x-1-2-3.zip
wAde5764030 WT 3 62B de5764030_slices_x-1-2-3.zip
wA7f1df342e WT 1 38A 7f1df342e_slices_1-2-3-x.zip
wA7f1df342e WT 2 52A 7f1df342e_slices_1-2-3-x.zip
wA7f1df342e WT 3 58B 7f1df342e_slices_1-2-3-x.zip
wA26c6708c8 WT 1 12A 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 2 52A 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 3 45B 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 4 52B 26c6708c8_slices_1-2-3-4.zip
wAe6cb830a1 WT 1 43A e6cb830a1_slices_x-1-2-3.zip
wAe6cb830a1 WT 2 58B e6cb830a1_slices_x-1-2-3.zip
wAe6cb830a1 WT 3 41B e6cb830a1_slices_x-1-2-3.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAb9db07673 mutant 1 46A b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 2 61A b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 3 55B b9db07673_slices_1-2-3-4.zip
mAb9db07673 mutant 4 52B b9db07673_slices_1-2-3-4.zip
mAd7d01f992 mutant 3 15B d7d01f992_slices_1-2-3-4.zip
mAd7d01f992 mutant 4 41B d7d01f992_slices_1-2-3-4.zip
mAa3eb6199b mutant 1 53A a3eb6199b_slices_x-1-2-3.zip
mAa3eb6199b mutant 2 38B a3eb6199b_slices_x-1-2-3.zip
mAa3eb6199b mutant 3 61B a3eb6199b_slices_x-1-2-3.zip
mA665387fba mutant 2 62A 665387fba_slices_1-2-3-x.zip
mA665387fba mutant 3 38B 665387fba_slices_1-2-3-x.zip
mA5fbe48d4a mutant 1 55A 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 2 54A 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 3 46B 5fbe48d4a_slices_1-2-3-4.zip
mA5fbe48d4a mutant 4 61B 5fbe48d4a_slices_1-2-3-4.zip
mAae169fe20 mutant 1 58A ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 2 62A ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 3 38B ae169fe20_slices_1-2-3-4.zip
mAae169fe20 mutant 4 62B ae169fe20_slices_1-2-3-4.zip
wA7ad2ce46d WT 1 58A 7ad2ce46d_slices_1-2-x-x.zip
wA7ad2ce46d WT 2 62A 7ad2ce46d_slices_1-2-x-x.zip
wAde5764030 WT 2 87B de5764030_slices_x-1-2-3.zip
wAde5764030 WT 3 62B de5764030_slices_x-1-2-3.zip
wA7f1df342e WT 1 38A 7f1df342e_slices_1-2-3-x.zip
wA7f1df342e WT 2 52A 7f1df342e_slices_1-2-3-x.zip
wA7f1df342e WT 3 58B 7f1df342e_slices_1-2-3-x.zip
wA26c6708c8 WT 1 12A 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 2 52A 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 3 45B 26c6708c8_slices_1-2-3-4.zip
wA26c6708c8 WT 4 52B 26c6708c8_slices_1-2-3-4.zip
wAe6cb830a1 WT 1 43A e6cb830a1_slices_x-1-2-3.zip
wAe6cb830a1 WT 2 58B e6cb830a1_slices_x-1-2-3.zip
wAe6cb830a1 WT 3 41B e6cb830a1_slices_x-1-2-3.zip

Data Files

File NameSizeChecksum (SHA256)
d7d01f992_slices_1-2-3-4.zip36413844d470b705d41842a77fa0daa5f73d21c4a325372b247af90e51e53bf3f1f90cc7
5fbe48d4a_slices_1-2-3-4.zip30458182ae82de5c248c82f0c2a79f59e35e05f63351206ab97570e36a4e289b3260d40e
26c6708c8_slices_1-2-3-4.zip393763739699efcb3177d245be1b5653594bba27f9f104afb0d51789b2d21724a967e227
665387fba_slices_1-2-3-x.zip36050056f0cd4c32169e54a2b65e5fc67a81409c4563080e15c9649d3352cbe4fd54fad3
e6cb830a1_slices_x-1-2-3.zip28606456218c0433b420a5f21c5d4a81172b7b257a226839506602b14936aaebe4505c3d
7ad2ce46d_slices_1-2-x-x.zip381082712c1b28c0bc480fc2c52c13a71fd310f0e6d2df95258e70f105be6bd0ca6449fc
7f1df342e_slices_1-2-3-x.zip47865573a5ea4bdd70ab38a3286ea3429d60f669a1081d0919df77b1dd7f2cb5307d3bc0
b9db07673_slices_1-2-3-4.zip33854106ebbf14479fea9e2031230e24d4d99ec20a9a7d92157d3524b7f9e0e67ccb2104
a3eb6199b_slices_x-1-2-3.zip316529383450979127f40bf8a7d604292b0d8f6933b415d559e2fe0f0a67bc96e33c1d0f
ae169fe20_slices_1-2-3-4.zip31432271e90e0080827cd94f3651992112b969e5b1bd5b89ba1d81cba560164815ccf528
de5764030_slices_x-1-2-3.zip547325543a39e8fe6a4cc23f10e4afd5f2dfc8367c1e38f2a94031ff76a907ee38885d54
© G2C 2014. The Genes to Cognition Programme received funding from The Wellcome Trust and the EU FP7 Framework Programmes:
EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

Cookies Policy | Terms and Conditions. This site is hosted by Edinburgh University and the Genes to Cognition Programme.