G2C::Electrophysiology

Kras knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices, with two of nine variables significantly impacted in these mutant slices. In the basal synaptic transmission experiment, max fEPSP amplitude was significantly increased in mutants. In the theta burst stimulation experiment, one electrophysiological variable, burst 8-10 ave amplitude vs burst 2-4, was significantly decreased in mutants. Paired pulse facilitation and long term potentiation experiments were unaffected. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Kras knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 21 (6) 3170 (130) 19 (5) 3660 (140) 0.67 0.021 *
PPF at 50ms, ampl ratio % 21 (6) 183.6 (3.2) 19 (5) 180.7 (3.4) 0.52 0.53
Burst 1, PPF at 10ms, ampl ratio % 21 (6) 208.5 (6) 19 (5) 186.9 (5.2) 0.038 * 0.078
Burst 1, EPSP3 depr, ampl ratio % 21 (6) 60.4 (3.1) 19 (5) 58.5 (2.3) 0.13 0.7
Burst 1, ave amplitude µV 21 (6) 1305 (57) 19 (5) 1552 (70) 0.52 0.02 *
Burst 2, ave amplitude µV 21 (6) 2170 (130) 19 (5) 2500 (130) 0.59 0.093
Burst 3, ave amplitude µV 21 (6) 2440 (140) 19 (5) 2730 (150) 0.61 0.15
Burst 4, ave amplitude µV 21 (6) 2500 (130) 19 (5) 2720 (140) 0.54 0.27
Burst 5, ave amplitude µV 21 (6) 2460 (130) 19 (5) 2660 (140) 0.54 0.29
Burst 6, ave amplitude µV 21 (6) 2390 (120) 19 (5) 2580 (130) 0.52 0.32
Burst 7, ave amplitude µV 21 (6) 2300 (120) 19 (5) 2470 (130) 0.51 0.36
Burst 8, ave amplitude µV 21 (6) 2230 (120) 19 (5) 2370 (120) 0.5 0.41
Burst 9, ave amplitude µV 21 (6) 2150 (120) 19 (5) 2300 (120) 0.49 0.41
Burst 10, ave amplitude µV 21 (6) 2100 (120) 19 (5) 2230 (120) 0.47 0.47
Burst 2-4 ave amplitude vs Burst 1 % 21 (6) 180.5 (4.1) 19 (5) 174.9 (5) 0.2 0.47
Burst 8-10 ave amplitude vs Burst 2-4 % 21 (6) 91.3 (1.3) 19 (5) 87.4 (1.2) 0.59 0.043 *
LTP vs PTP, amplitude ratio % 21 (6) 82.4 (1.1) 19 (5) 81.5 (1.1) 0.22 0.62
LTP based on amplitude % 21 (6) 182.1 (3.8) 19 (5) 168.5 (4.5) 0.19 0.077

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 21 (6) 3170 (130) 19 (5) 3660 (140) 0.67 0.021 *

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 21 (6) 183.6 (3.2) 19 (5) 180.7 (3.4) 0.52 0.53

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 21 (6) 208.5 (6) 19 (5) 186.9 (5.2) 0.038 * 0.078
Burst 1, EPSP3 depr, ampl ratio % 21 (6) 60.4 (3.1) 19 (5) 58.5 (2.3) 0.13 0.7
Burst 1, ave amplitude µV 21 (6) 1305 (57) 19 (5) 1552 (70) 0.52 0.02 *
Burst 2, ave amplitude µV 21 (6) 2170 (130) 19 (5) 2500 (130) 0.59 0.093
Burst 3, ave amplitude µV 21 (6) 2440 (140) 19 (5) 2730 (150) 0.61 0.15
Burst 4, ave amplitude µV 21 (6) 2500 (130) 19 (5) 2720 (140) 0.54 0.27
Burst 5, ave amplitude µV 21 (6) 2460 (130) 19 (5) 2660 (140) 0.54 0.29
Burst 6, ave amplitude µV 21 (6) 2390 (120) 19 (5) 2580 (130) 0.52 0.32
Burst 7, ave amplitude µV 21 (6) 2300 (120) 19 (5) 2470 (130) 0.51 0.36
Burst 8, ave amplitude µV 21 (6) 2230 (120) 19 (5) 2370 (120) 0.5 0.41
Burst 9, ave amplitude µV 21 (6) 2150 (120) 19 (5) 2300 (120) 0.49 0.41
Burst 10, ave amplitude µV 21 (6) 2100 (120) 19 (5) 2230 (120) 0.47 0.47
Burst 2-4 ave amplitude vs Burst 1 % 21 (6) 180.5 (4.1) 19 (5) 174.9 (5) 0.2 0.47
Burst 8-10 ave amplitude vs Burst 2-4 % 21 (6) 91.3 (1.3) 19 (5) 87.4 (1.2) 0.59 0.043 *

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 21 (6) 82.4 (1.1) 19 (5) 81.5 (1.1) 0.22 0.62
LTP based on amplitude % 21 (6) 182.1 (3.8) 19 (5) 168.5 (4.5) 0.19 0.077

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT6
mutant6

 

Individual Mice

Mouse IDGenotype
wAe3a75b789WT
wAf250f65b0WT
wA29292615cWT
wA07d3beeb7WT
wA1925df183WT
wAcb953bbe6WT
mAb90d0aa84mutant
mA3753a3ef4mutant
mA882134c5cmutant
mAda9311b35mutant
mA22b1cc2d7mutant
mAf29b2de8fmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAb90d0aa84 mutant 1 53A b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 2 53A b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 3 58B b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 4 62B b90d0aa84_slices_1-2-3-4.zip
mAf29b2de8f mutant 1 58A f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 2 52A f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 3 38B f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 4 52B f29b2de8f_slices_1-2-3-4.zip
mAda9311b35 mutant 1 46A da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 2 52A da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 3 55B da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 4 61B da9311b35_slices_1-2-3-4.zip
mA882134c5c mutant 1 12A 882134c5c_slices_1-2-x-3.zip
mA882134c5c mutant 2 52A 882134c5c_slices_1-2-x-3.zip
mA882134c5c mutant 3 42B 882134c5c_slices_1-2-x-3.zip
mA22b1cc2d7 mutant 1 58A 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 2 52A 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 3 58B 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 4 62B 22b1cc2d7_slices_1-2-3-4.zip
mA3753a3ef4 mutant 1 58A 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 2 41A 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 3 55B 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 4 52B 3753a3ef4_slices_1-2-3-4.zip
wAf250f65b0 WT 1 55A f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 2 52A f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 3 46B f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 4 61B f250f65b0_slices_1-2-3-4.zip
wAe3a75b789 WT 1 55A e3a75b789_slices_1-2-x-3.zip
wAe3a75b789 WT 2 62A e3a75b789_slices_1-2-x-3.zip
wAe3a75b789 WT 3 41B e3a75b789_slices_1-2-x-3.zip
wA07d3beeb7 WT 1 46A 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 2 87A 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 3 55B 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 4 62B 07d3beeb7_slices_1-2-3-4.zip
wAcb953bbe6 WT 1 12A cb953bbe6_slices_1-2-x-3.zip
wAcb953bbe6 WT 2 42A cb953bbe6_slices_1-2-x-3.zip
wAcb953bbe6 WT 3 52B cb953bbe6_slices_1-2-x-3.zip
wA1925df183 WT 1 46A 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 2 87A 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 3 45B 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 4 62B 1925df183_slices_1-2-3-4.zip
wA29292615c WT 2 73A 29292615c_slices_1-2-3-4.zip
wA29292615c WT 3 57B 29292615c_slices_1-2-3-4.zip
wA29292615c WT 4 43B 29292615c_slices_1-2-3-4.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAb90d0aa84 mutant 1 53A b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 2 53A b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 3 58B b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 4 62B b90d0aa84_slices_1-2-3-4.zip
mAf29b2de8f mutant 1 58A f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 2 52A f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 3 38B f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 4 52B f29b2de8f_slices_1-2-3-4.zip
mAda9311b35 mutant 1 46A da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 2 52A da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 3 55B da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 4 61B da9311b35_slices_1-2-3-4.zip
mA882134c5c mutant 1 12A 882134c5c_slices_1-2-x-3.zip
mA882134c5c mutant 2 52A 882134c5c_slices_1-2-x-3.zip
mA882134c5c mutant 3 42B 882134c5c_slices_1-2-x-3.zip
mA22b1cc2d7 mutant 1 58A 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 2 52A 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 3 58B 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 4 62B 22b1cc2d7_slices_1-2-3-4.zip
mA3753a3ef4 mutant 1 58A 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 2 41A 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 3 55B 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 4 52B 3753a3ef4_slices_1-2-3-4.zip
wAf250f65b0 WT 1 55A f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 2 52A f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 3 46B f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 4 61B f250f65b0_slices_1-2-3-4.zip
wAe3a75b789 WT 1 55A e3a75b789_slices_1-2-x-3.zip
wAe3a75b789 WT 2 62A e3a75b789_slices_1-2-x-3.zip
wAe3a75b789 WT 3 41B e3a75b789_slices_1-2-x-3.zip
wA07d3beeb7 WT 1 46A 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 2 87A 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 3 55B 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 4 62B 07d3beeb7_slices_1-2-3-4.zip
wAcb953bbe6 WT 1 12A cb953bbe6_slices_1-2-x-3.zip
wAcb953bbe6 WT 2 42A cb953bbe6_slices_1-2-x-3.zip
wAcb953bbe6 WT 3 52B cb953bbe6_slices_1-2-x-3.zip
wA1925df183 WT 1 46A 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 2 87A 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 3 45B 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 4 62B 1925df183_slices_1-2-3-4.zip
wA29292615c WT 2 73A 29292615c_slices_1-2-3-4.zip
wA29292615c WT 3 57B 29292615c_slices_1-2-3-4.zip
wA29292615c WT 4 43B 29292615c_slices_1-2-3-4.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAb90d0aa84 mutant 1 53A b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 2 53A b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 3 58B b90d0aa84_slices_1-2-3-4.zip
mAb90d0aa84 mutant 4 62B b90d0aa84_slices_1-2-3-4.zip
mAf29b2de8f mutant 1 58A f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 2 52A f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 3 38B f29b2de8f_slices_1-2-3-4.zip
mAf29b2de8f mutant 4 52B f29b2de8f_slices_1-2-3-4.zip
mAda9311b35 mutant 1 46A da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 2 52A da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 3 55B da9311b35_slices_1-2-3-4.zip
mAda9311b35 mutant 4 61B da9311b35_slices_1-2-3-4.zip
mA882134c5c mutant 1 12A 882134c5c_slices_1-2-x-3.zip
mA882134c5c mutant 2 52A 882134c5c_slices_1-2-x-3.zip
mA882134c5c mutant 3 42B 882134c5c_slices_1-2-x-3.zip
mA22b1cc2d7 mutant 1 58A 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 2 52A 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 3 58B 22b1cc2d7_slices_1-2-3-4.zip
mA22b1cc2d7 mutant 4 62B 22b1cc2d7_slices_1-2-3-4.zip
mA3753a3ef4 mutant 1 58A 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 2 41A 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 3 55B 3753a3ef4_slices_1-2-3-4.zip
mA3753a3ef4 mutant 4 52B 3753a3ef4_slices_1-2-3-4.zip
wAf250f65b0 WT 1 55A f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 2 52A f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 3 46B f250f65b0_slices_1-2-3-4.zip
wAf250f65b0 WT 4 61B f250f65b0_slices_1-2-3-4.zip
wAe3a75b789 WT 1 55A e3a75b789_slices_1-2-x-3.zip
wAe3a75b789 WT 2 62A e3a75b789_slices_1-2-x-3.zip
wAe3a75b789 WT 3 41B e3a75b789_slices_1-2-x-3.zip
wA07d3beeb7 WT 1 46A 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 2 87A 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 3 55B 07d3beeb7_slices_1-2-3-4.zip
wA07d3beeb7 WT 4 62B 07d3beeb7_slices_1-2-3-4.zip
wAcb953bbe6 WT 1 12A cb953bbe6_slices_1-2-x-3.zip
wAcb953bbe6 WT 2 42A cb953bbe6_slices_1-2-x-3.zip
wAcb953bbe6 WT 3 52B cb953bbe6_slices_1-2-x-3.zip
wA1925df183 WT 1 46A 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 2 87A 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 3 45B 1925df183_slices_1-2-3-4.zip
wA1925df183 WT 4 62B 1925df183_slices_1-2-3-4.zip
wA29292615c WT 2 73A 29292615c_slices_1-2-3-4.zip
wA29292615c WT 3 57B 29292615c_slices_1-2-3-4.zip
wA29292615c WT 4 43B 29292615c_slices_1-2-3-4.zip

Data Files

File NameSizeChecksum (SHA256)
3753a3ef4_slices_1-2-3-4.zip43122150b2647ed04e0b3d68310e2ddcd02c6b827d5c1de44d434bf24c7d08339d6bc556
f29b2de8f_slices_1-2-3-4.zip42799670aa98d16de420d1ca55ea739ad734e1a9df5ff2f250a781c5f48b6c07db4a7916
29292615c_slices_1-2-3-4.zip45656654d47f69a5f0337fe4b201ac62f28fdbab52598cbd657b2f9cbb923cf9b5da63ec
da9311b35_slices_1-2-3-4.zip34964458c182876cf40ed98e53cf05be829b9c15e1c938a702d7aa8cb29562a022f46b26
f250f65b0_slices_1-2-3-4.zip33063414f168fd861c0045220a8296a847983293b9f59367582a4445d7e325e1447c7aed
1925df183_slices_1-2-3-4.zip32596477224d83fbdad5450380e18a0498d2a35f2316d7a4ecfeeb7f93fae2afea896baf
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