G2C::Electrophysiology

S-SCAM knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices. With heterozygous genotype and no electrophysiological variables significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the S-SCAM knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 15 (5) 2890 (170) 15 (5) 2610 (120) 0.3 0.29
PPF at 50ms, ampl ratio % 15 (5) 173.4 (5.4) 15 (5) 165.3 (5.1) 0.33 0.36
Burst 1, PPF at 10ms, ampl ratio % 15 (5) 194 (8.8) 15 (5) 203.5 (8.7) 0.48 0.47
Burst 1, EPSP3 depr, ampl ratio % 15 (5) 66.2 (3.6) 15 (5) 62.6 (3.8) 0.17 0.59
Burst 1, ave amplitude µV 15 (5) 1192 (53) 15 (5) 1062 (37) 0.57 0.079
Burst 2, ave amplitude µV 15 (5) 1800 (130) 15 (5) 1470 (130) 0.46 0.14
Burst 3, ave amplitude µV 15 (5) 2070 (160) 15 (5) 1660 (160) 0.29 0.15
Burst 4, ave amplitude µV 15 (5) 2190 (150) 15 (5) 1760 (150) 0.33 0.12
Burst 5, ave amplitude µV 15 (5) 2220 (140) 15 (5) 1770 (140) 0.32 0.091
Burst 6, ave amplitude µV 15 (5) 2170 (140) 15 (5) 1720 (140) 0.33 0.079
Burst 7, ave amplitude µV 15 (5) 2130 (140) 15 (5) 1660 (140) 0.34 0.065
Burst 8, ave amplitude µV 15 (5) 2080 (140) 15 (5) 1600 (140) 0.36 0.065
Burst 9, ave amplitude µV 15 (5) 2040 (150) 15 (5) 1540 (140) 0.36 0.06
Burst 10, ave amplitude µV 15 (5) 2000 (150) 15 (5) 1500 (140) 0.34 0.066
Burst 2-4 ave amplitude vs Burst 1 % 15 (5) 172.7 (7.4) 15 (5) 161.2 (9.5) 0.26 0.44
Burst 8-10 ave amplitude vs Burst 2-4 % 15 (5) 98.5 (2) 15 (5) 93 (2) 0.49 0.097
LTP vs PTP, amplitude ratio % 15 (5) 76.1 (2.2) 15 (5) 74.6 (1.4) 0.86 0.44
LTP based on amplitude % 15 (5) 174.2 (6) 15 (5) 168.1 (7.6) 0.83 0.44

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 15 (5) 2890 (170) 15 (5) 2610 (120) 0.3 0.29

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 15 (5) 173.4 (5.4) 15 (5) 165.3 (5.1) 0.33 0.36

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 15 (5) 194 (8.8) 15 (5) 203.5 (8.7) 0.48 0.47
Burst 1, EPSP3 depr, ampl ratio % 15 (5) 66.2 (3.6) 15 (5) 62.6 (3.8) 0.17 0.59
Burst 1, ave amplitude µV 15 (5) 1192 (53) 15 (5) 1062 (37) 0.57 0.079
Burst 2, ave amplitude µV 15 (5) 1800 (130) 15 (5) 1470 (130) 0.46 0.14
Burst 3, ave amplitude µV 15 (5) 2070 (160) 15 (5) 1660 (160) 0.29 0.15
Burst 4, ave amplitude µV 15 (5) 2190 (150) 15 (5) 1760 (150) 0.33 0.12
Burst 5, ave amplitude µV 15 (5) 2220 (140) 15 (5) 1770 (140) 0.32 0.091
Burst 6, ave amplitude µV 15 (5) 2170 (140) 15 (5) 1720 (140) 0.33 0.079
Burst 7, ave amplitude µV 15 (5) 2130 (140) 15 (5) 1660 (140) 0.34 0.065
Burst 8, ave amplitude µV 15 (5) 2080 (140) 15 (5) 1600 (140) 0.36 0.065
Burst 9, ave amplitude µV 15 (5) 2040 (150) 15 (5) 1540 (140) 0.36 0.06
Burst 10, ave amplitude µV 15 (5) 2000 (150) 15 (5) 1500 (140) 0.34 0.066
Burst 2-4 ave amplitude vs Burst 1 % 15 (5) 172.7 (7.4) 15 (5) 161.2 (9.5) 0.26 0.44
Burst 8-10 ave amplitude vs Burst 2-4 % 15 (5) 98.5 (2) 15 (5) 93 (2) 0.49 0.097

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 15 (5) 76.1 (2.2) 15 (5) 74.6 (1.4) 0.86 0.44
LTP based on amplitude % 15 (5) 174.2 (6) 15 (5) 168.1 (7.6) 0.83 0.44

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wA4c0260de4WT
wB152db590fWT
wA57ff06855WT
wBadd57fe1bWT
wA5b08b0462WT
mAc8467617emutant
mAec9d0af07mutant
mB5b08b0462mutant
mAadd57fe1bmutant
mA152db590fmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB5b08b0462 mutant 4 12A 5b08b0462_slices_3-x-4-5.zip
mAec9d0af07 mutant 1 46A ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 2 52A ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 4 62B ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 5 46B ec9d0af07_slices_x-x-5-6.zip
mAc8467617e mutant 1 61A c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 2 58B c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 3 52B c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 5 42A c8467617e_slices_4-5-6-x.zip
mAc8467617e mutant 6 58B c8467617e_slices_4-5-6-x.zip
mAadd57fe1b mutant 1 52A add57fe1b_slices_x-1-2-3.zip
mAadd57fe1b mutant 2 46B add57fe1b_slices_x-1-2-3.zip
mAadd57fe1b mutant 3 52B add57fe1b_slices_x-1-2-3.zip
mA152db590f mutant 1 55A 152db590f_slices_1-2-3-4.zip
mA152db590f mutant 2 52A 152db590f_slices_1-2-3-4.zip
mA152db590f mutant 4 52B 152db590f_slices_1-2-3-4.zip
wA5b08b0462 WT 1 12B 5b08b0462_slices_x-x-1-2.zip
wA5b08b0462 WT 2 52B 5b08b0462_slices_x-x-1-2.zip
wA4c0260de4 WT 1 12A 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 2 43A 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 3 46B 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 4 54B 4c0260de4_slices_1-2-3-4.zip
wA57ff06855 WT 1 61A 57ff06855_slices_x-1-2-3.zip
wA57ff06855 WT 2 46B 57ff06855_slices_x-1-2-3.zip
wA57ff06855 WT 3 54B 57ff06855_slices_x-1-2-3.zip
wBadd57fe1b WT 4 15A add57fe1b_slices_4-5-6-x.zip
wBadd57fe1b WT 5 52B add57fe1b_slices_4-5-6-x.zip
wBadd57fe1b WT 6 58B add57fe1b_slices_4-5-6-x.zip
wB152db590f WT 5 46A 152db590f_slices_5-6-7-x.zip
wB152db590f WT 6 42A 152db590f_slices_5-6-7-x.zip
wB152db590f WT 7 46B 152db590f_slices_5-6-7-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB5b08b0462 mutant 4 12A 5b08b0462_slices_3-x-4-5.zip
mAec9d0af07 mutant 1 46A ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 2 52A ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 4 62B ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 5 46B ec9d0af07_slices_x-x-5-6.zip
mAc8467617e mutant 1 61A c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 2 58B c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 3 52B c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 5 42A c8467617e_slices_4-5-6-x.zip
mAc8467617e mutant 6 58B c8467617e_slices_4-5-6-x.zip
mAadd57fe1b mutant 1 52A add57fe1b_slices_x-1-2-3.zip
mAadd57fe1b mutant 2 46B add57fe1b_slices_x-1-2-3.zip
mAadd57fe1b mutant 3 52B add57fe1b_slices_x-1-2-3.zip
mA152db590f mutant 1 55A 152db590f_slices_1-2-3-4.zip
mA152db590f mutant 2 52A 152db590f_slices_1-2-3-4.zip
mA152db590f mutant 4 52B 152db590f_slices_1-2-3-4.zip
wA5b08b0462 WT 1 12B 5b08b0462_slices_x-x-1-2.zip
wA5b08b0462 WT 2 52B 5b08b0462_slices_x-x-1-2.zip
wA4c0260de4 WT 1 12A 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 2 43A 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 3 46B 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 4 54B 4c0260de4_slices_1-2-3-4.zip
wA57ff06855 WT 1 61A 57ff06855_slices_x-1-2-3.zip
wA57ff06855 WT 2 46B 57ff06855_slices_x-1-2-3.zip
wA57ff06855 WT 3 54B 57ff06855_slices_x-1-2-3.zip
wBadd57fe1b WT 4 15A add57fe1b_slices_4-5-6-x.zip
wBadd57fe1b WT 5 52B add57fe1b_slices_4-5-6-x.zip
wBadd57fe1b WT 6 58B add57fe1b_slices_4-5-6-x.zip
wB152db590f WT 5 46A 152db590f_slices_5-6-7-x.zip
wB152db590f WT 6 42A 152db590f_slices_5-6-7-x.zip
wB152db590f WT 7 46B 152db590f_slices_5-6-7-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB5b08b0462 mutant 4 12A 5b08b0462_slices_3-x-4-5.zip
mAec9d0af07 mutant 1 46A ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 2 52A ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 4 62B ec9d0af07_slices_1-2-3-4.zip
mAec9d0af07 mutant 5 46B ec9d0af07_slices_x-x-5-6.zip
mAc8467617e mutant 1 61A c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 2 58B c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 3 52B c8467617e_slices_x-1-2-3.zip
mAc8467617e mutant 5 42A c8467617e_slices_4-5-6-x.zip
mAc8467617e mutant 6 58B c8467617e_slices_4-5-6-x.zip
mAadd57fe1b mutant 1 52A add57fe1b_slices_x-1-2-3.zip
mAadd57fe1b mutant 2 46B add57fe1b_slices_x-1-2-3.zip
mAadd57fe1b mutant 3 52B add57fe1b_slices_x-1-2-3.zip
mA152db590f mutant 1 55A 152db590f_slices_1-2-3-4.zip
mA152db590f mutant 2 52A 152db590f_slices_1-2-3-4.zip
mA152db590f mutant 4 52B 152db590f_slices_1-2-3-4.zip
wA5b08b0462 WT 1 12B 5b08b0462_slices_x-x-1-2.zip
wA5b08b0462 WT 2 52B 5b08b0462_slices_x-x-1-2.zip
wA4c0260de4 WT 1 12A 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 2 43A 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 3 46B 4c0260de4_slices_1-2-3-4.zip
wA4c0260de4 WT 4 54B 4c0260de4_slices_1-2-3-4.zip
wA57ff06855 WT 1 61A 57ff06855_slices_x-1-2-3.zip
wA57ff06855 WT 2 46B 57ff06855_slices_x-1-2-3.zip
wA57ff06855 WT 3 54B 57ff06855_slices_x-1-2-3.zip
wBadd57fe1b WT 4 15A add57fe1b_slices_4-5-6-x.zip
wBadd57fe1b WT 5 52B add57fe1b_slices_4-5-6-x.zip
wBadd57fe1b WT 6 58B add57fe1b_slices_4-5-6-x.zip
wB152db590f WT 5 46A 152db590f_slices_5-6-7-x.zip
wB152db590f WT 6 42A 152db590f_slices_5-6-7-x.zip
wB152db590f WT 7 46B 152db590f_slices_5-6-7-x.zip

Data Files

File NameSizeChecksum (SHA256)
4c0260de4_slices_1-2-3-4.zip35388035eca967d53f041ff8a47d468a5db9bd3cce830cf7e2112d51237017e04b84e7e3
c8467617e_slices_x-1-2-3.zip33459831149225f791bfbada20b8f04250618e0c3cc33109465e20f2902ec2742e55d057
ec9d0af07_slices_x-x-5-6.zip415748326c5e779a04c19d9e9001babd7bb932826e36e53b9ccf10551afcfd14d0fbc7a6
c8467617e_slices_4-5-6-x.zip620132669abb1b6de51e503018e60d14b65f4d2b28b8e44ff836126facf023c514a73f37
ec9d0af07_slices_1-2-3-4.zip35810986ac0e618c470c51dec64c7fd394cb18907f6703dcc21ef0bacd9fa2481cef7160
5b08b0462_slices_x-x-1-2.zip365869645120aa3a2f8d0cfaca1f68e6320cff3ba1b506c4930c375007d728af44ffee82
add57fe1b_slices_4-5-6-x.zip443211104b5d023959831d792669410b284ca58aed5d566f49066d3385352bb41024bf56
5b08b0462_slices_3-x-4-5.zip41811343739dfa683939ea62315a6511ca993fbb14370fb6b391b5e8ecad6d04d77c8dce
57ff06855_slices_x-1-2-3.zip36391286a9e29db0019a1e71e2213d2d3f972f80922be647ad5a291961e816ac583668d0
add57fe1b_slices_x-1-2-3.zip327494331febfd3a79dc87aa3c16c5c8f2aec53cd5bc310f66a30c1a19520dc1134af245
152db590f_slices_1-2-3-4.zip350814298593bd2fa17f16932ed3f3fea3a718a7d1fa33074e310883e4c3b7d264b2ad57
152db590f_slices_5-6-7-x.zip4201541331634fb65342944d967bafd278a7a6251aa5b79f81c5c882d962050c75c9070c
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EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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