G2C::Electrophysiology

Iqsec1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with no electrophysiological variables significantly affected by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Iqsec1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 18 (5) 2840 (180) 24 (7) 3080 (150) 0.37 0.34
PPF at 50ms, ampl ratio % 18 (5) 170.5 (5) 24 (7) 175.7 (4.7) 0.94 0.28
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 173.3 (10.8) 24 (7) 179.9 (6.1) 0.54 0.56
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 64.5 (3.3) 24 (7) 59.6 (2.4) 0.32 0.28
Burst 1, ave amplitude µV 17 (5) 1304 (74) 24 (7) 1323 (44) 0.062 0.86
Burst 2, ave amplitude µV 17 (5) 2100 (210) 24 (7) 1977 (98) 0.086 0.65
Burst 3, ave amplitude µV 17 (5) 2330 (220) 24 (7) 2270 (110) 0.12 0.86
Burst 4, ave amplitude µV 17 (5) 2440 (210) 24 (7) 2400 (120) 0.14 0.91
Burst 5, ave amplitude µV 17 (5) 2430 (200) 24 (7) 2380 (110) 0.15 0.84
Burst 6, ave amplitude µV 17 (5) 2350 (180) 24 (7) 2310 (100) 0.17 0.85
Burst 7, ave amplitude µV 17 (5) 2266 (174) 24 (7) 2210 (97) 0.16 0.8
Burst 8, ave amplitude µV 17 (5) 2174 (163) 24 (7) 2114 (92) 0.2 0.77
Burst 9, ave amplitude µV 17 (5) 2095 (159) 23 (7) 2073 (86) 0.085 0.92
Burst 10, ave amplitude µV 17 (5) 2028 (154) 23 (7) 2005 (86) 0.089 0.91
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 173.9 (6.5) 24 (7) 170 (5.8) 0.52 0.66
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 92.9 (1.7) 23 (7) 91.5 (1.1) 0.06 0.59
LTP vs PTP, amplitude ratio % 17 (5) 82.6 (1.5) 24 (7) 78.8 (1.4) 0.37 0.11
LTP based on amplitude % 17 (5) 179.8 (7.4) 24 (7) 170.5 (5.7) 0.83 0.23

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 18 (5) 2840 (180) 24 (7) 3080 (150) 0.37 0.34

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 18 (5) 170.5 (5) 24 (7) 175.7 (4.7) 0.94 0.28

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 173.3 (10.8) 24 (7) 179.9 (6.1) 0.54 0.56
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 64.5 (3.3) 24 (7) 59.6 (2.4) 0.32 0.28
Burst 1, ave amplitude µV 17 (5) 1304 (74) 24 (7) 1323 (44) 0.062 0.86
Burst 2, ave amplitude µV 17 (5) 2100 (210) 24 (7) 1977 (98) 0.086 0.65
Burst 3, ave amplitude µV 17 (5) 2330 (220) 24 (7) 2270 (110) 0.12 0.86
Burst 4, ave amplitude µV 17 (5) 2440 (210) 24 (7) 2400 (120) 0.14 0.91
Burst 5, ave amplitude µV 17 (5) 2430 (200) 24 (7) 2380 (110) 0.15 0.84
Burst 6, ave amplitude µV 17 (5) 2350 (180) 24 (7) 2310 (100) 0.17 0.85
Burst 7, ave amplitude µV 17 (5) 2266 (174) 24 (7) 2210 (97) 0.16 0.8
Burst 8, ave amplitude µV 17 (5) 2174 (163) 24 (7) 2114 (92) 0.2 0.77
Burst 9, ave amplitude µV 17 (5) 2095 (159) 23 (7) 2073 (86) 0.085 0.92
Burst 10, ave amplitude µV 17 (5) 2028 (154) 23 (7) 2005 (86) 0.089 0.91
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 173.9 (6.5) 24 (7) 170 (5.8) 0.52 0.66
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 92.9 (1.7) 23 (7) 91.5 (1.1) 0.06 0.59

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 17 (5) 82.6 (1.5) 24 (7) 78.8 (1.4) 0.37 0.11
LTP based on amplitude % 17 (5) 179.8 (7.4) 24 (7) 170.5 (5.7) 0.83 0.23

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant7

 

Individual Mice

Mouse IDGenotype
wA317a7381cWT
wB9f8a78f31WT
wB28eef77cbWT
wA1cf94f536WT
wAf36dde395WT
mA32b0c3198mutant
mB669e5dbc5mutant
mBfea533069mutant
mA221b118f8mutant
mB317a7381cmutant
mAd3265f5d1mutant
mB32b0c3198mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA221b118f8 mutant 1 54A 221b118f8_slices_x-1-2-x.zip
mA221b118f8 mutant 2 46B 221b118f8_slices_x-1-2-x.zip
mB317a7381c mutant 5 53A 317a7381c_slices_5-x-6-7.zip
mB317a7381c mutant 6 15B 317a7381c_slices_5-x-6-7.zip
mB317a7381c mutant 7 52B 317a7381c_slices_5-x-6-7.zip
mAd3265f5d1 mutant 1 46A d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 2 52A d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 3 46B d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 4 52B d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 5 46A d3265f5d1_slices_5-x-x-x.zip
mB669e5dbc5 mutant 5 46A 669e5dbc5_slices_5-6-7-x.zip
mB669e5dbc5 mutant 6 41A 669e5dbc5_slices_5-6-7-x.zip
mB669e5dbc5 mutant 7 15B 669e5dbc5_slices_5-6-7-x.zip
mA32b0c3198 mutant 1 12A 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 2 52A 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 3 46B 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 4 52B 32b0c3198_slices_1-2-3-4.zip
mB32b0c3198 mutant 5 58A 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 6 52A 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 7 15B 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 8 52B 32b0c3198_slices_5-6-7-8.zip
mBfea533069 mutant 5 46A fea533069_slices_5-6-7-x.zip
mBfea533069 mutant 6 52A fea533069_slices_5-6-7-x.zip
mBfea533069 mutant 7 46B fea533069_slices_5-6-7-x.zip
wAf36dde395 WT 1 38A f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 2 52A f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 3 15B f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 4 61B f36dde395_slices_1-2-3-4.zip
wB28eef77cb WT 5 46A 28eef77cb_slices_5-x-6-7.zip
wB28eef77cb WT 6 55B 28eef77cb_slices_5-x-6-7.zip
wB28eef77cb WT 7 43B 28eef77cb_slices_5-x-6-7.zip
wB9f8a78f31 WT 3 38B 9f8a78f31_slices_1-2-3-4.zip
wB9f8a78f31 WT 5 12A 9f8a78f31_slices_5-x-6-7.zip
wB9f8a78f31 WT 6 43B 9f8a78f31_slices_5-x-6-7.zip
wB9f8a78f31 WT 7 58B 9f8a78f31_slices_5-x-6-7.zip
wA317a7381c WT 1 12A 317a7381c_slices_1-2-3-4.zip
wA317a7381c WT 2 52A 317a7381c_slices_1-2-3-4.zip
wA317a7381c WT 3 46B 317a7381c_slices_1-2-3-4.zip
wA1cf94f536 WT 1 46A 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 2 52A 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 3 15B 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 4 52B 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 5 1cf94f536_slices_5-x-x-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA221b118f8 mutant 1 54A 221b118f8_slices_x-1-2-x.zip
mA221b118f8 mutant 2 46B 221b118f8_slices_x-1-2-x.zip
mB317a7381c mutant 5 53A 317a7381c_slices_5-x-6-7.zip
mB317a7381c mutant 6 15B 317a7381c_slices_5-x-6-7.zip
mB317a7381c mutant 7 52B 317a7381c_slices_5-x-6-7.zip
mAd3265f5d1 mutant 1 46A d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 2 52A d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 3 46B d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 4 52B d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 5 46A d3265f5d1_slices_5-x-x-x.zip
mB669e5dbc5 mutant 5 46A 669e5dbc5_slices_5-6-7-x.zip
mB669e5dbc5 mutant 6 41A 669e5dbc5_slices_5-6-7-x.zip
mB669e5dbc5 mutant 7 15B 669e5dbc5_slices_5-6-7-x.zip
mA32b0c3198 mutant 1 12A 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 2 52A 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 3 46B 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 4 52B 32b0c3198_slices_1-2-3-4.zip
mB32b0c3198 mutant 5 58A 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 6 52A 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 7 15B 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 8 52B 32b0c3198_slices_5-6-7-8.zip
mBfea533069 mutant 5 46A fea533069_slices_5-6-7-x.zip
mBfea533069 mutant 6 52A fea533069_slices_5-6-7-x.zip
mBfea533069 mutant 7 46B fea533069_slices_5-6-7-x.zip
wAf36dde395 WT 1 38A f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 2 52A f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 3 15B f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 4 61B f36dde395_slices_1-2-3-4.zip
wB28eef77cb WT 5 46A 28eef77cb_slices_5-x-6-7.zip
wB28eef77cb WT 6 55B 28eef77cb_slices_5-x-6-7.zip
wB28eef77cb WT 7 43B 28eef77cb_slices_5-x-6-7.zip
wB9f8a78f31 WT 3 38B 9f8a78f31_slices_1-2-3-4.zip
wB9f8a78f31 WT 5 12A 9f8a78f31_slices_5-x-6-7.zip
wB9f8a78f31 WT 6 43B 9f8a78f31_slices_5-x-6-7.zip
wB9f8a78f31 WT 7 58B 9f8a78f31_slices_5-x-6-7.zip
wA317a7381c WT 1 12A 317a7381c_slices_1-2-3-4.zip
wA317a7381c WT 2 52A 317a7381c_slices_1-2-3-4.zip
wA317a7381c WT 3 46B 317a7381c_slices_1-2-3-4.zip
wA1cf94f536 WT 1 46A 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 2 52A 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 3 15B 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 4 52B 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 5 1cf94f536_slices_5-x-x-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA221b118f8 mutant 1 54A 221b118f8_slices_x-1-2-x.zip
mA221b118f8 mutant 2 46B 221b118f8_slices_x-1-2-x.zip
mB317a7381c mutant 5 53A 317a7381c_slices_5-x-6-7.zip
mB317a7381c mutant 6 15B 317a7381c_slices_5-x-6-7.zip
mB317a7381c mutant 7 52B 317a7381c_slices_5-x-6-7.zip
mAd3265f5d1 mutant 1 46A d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 2 52A d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 3 46B d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 4 52B d3265f5d1_slices_1-2-3-4.zip
mAd3265f5d1 mutant 5 46A d3265f5d1_slices_5-x-x-x.zip
mB669e5dbc5 mutant 5 46A 669e5dbc5_slices_5-6-7-x.zip
mB669e5dbc5 mutant 6 41A 669e5dbc5_slices_5-6-7-x.zip
mB669e5dbc5 mutant 7 15B 669e5dbc5_slices_5-6-7-x.zip
mA32b0c3198 mutant 1 12A 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 2 52A 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 3 46B 32b0c3198_slices_1-2-3-4.zip
mA32b0c3198 mutant 4 52B 32b0c3198_slices_1-2-3-4.zip
mB32b0c3198 mutant 5 58A 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 6 52A 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 7 15B 32b0c3198_slices_5-6-7-8.zip
mB32b0c3198 mutant 8 52B 32b0c3198_slices_5-6-7-8.zip
mBfea533069 mutant 5 46A fea533069_slices_5-6-7-x.zip
mBfea533069 mutant 6 52A fea533069_slices_5-6-7-x.zip
mBfea533069 mutant 7 46B fea533069_slices_5-6-7-x.zip
wAf36dde395 WT 1 38A f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 2 52A f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 3 15B f36dde395_slices_1-2-3-4.zip
wAf36dde395 WT 4 61B f36dde395_slices_1-2-3-4.zip
wB28eef77cb WT 5 46A 28eef77cb_slices_5-x-6-7.zip
wB28eef77cb WT 7 43B 28eef77cb_slices_5-x-6-7.zip
wB9f8a78f31 WT 3 38B 9f8a78f31_slices_1-2-3-4.zip
wB9f8a78f31 WT 5 12A 9f8a78f31_slices_5-x-6-7.zip
wB9f8a78f31 WT 6 43B 9f8a78f31_slices_5-x-6-7.zip
wB9f8a78f31 WT 7 58B 9f8a78f31_slices_5-x-6-7.zip
wA317a7381c WT 1 12A 317a7381c_slices_1-2-3-4.zip
wA317a7381c WT 2 52A 317a7381c_slices_1-2-3-4.zip
wA317a7381c WT 3 46B 317a7381c_slices_1-2-3-4.zip
wA1cf94f536 WT 1 46A 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 2 52A 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 3 15B 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 4 52B 1cf94f536_slices_1-2-3-4.zip
wA1cf94f536 WT 5 1cf94f536_slices_5-x-x-x.zip

Data Files

File NameSizeChecksum (SHA256)
669e5dbc5_slices_5-6-7-x.zip5535402444dad0c218eecf9318caeff8a3c602988dc1a61ce7240ff67bb800bc23218f34
9f8a78f31_slices_5-x-6-7.zip5398954529b92071ed6e01b30886ca9a9339fa8a8ca932ac0af2bdd2f2bd415eecfdd248
32b0c3198_slices_5-6-7-8.zip5014744161e6265d57884f0ecbbe46067192bf87b4916d12f120e377a5e199ed83590b17
1cf94f536_slices_5-x-x-x.zip44965199dccfdc5ee2f4faa5d23eebb9dd1217fb7ea341955b7bd1541ed47ea17f1ebe65
28eef77cb_slices_5-x-6-7.zip57951049fda55b1a61d60fc26629063b2dc2cbbb0a60ecf86eed95ab259c1d8f79690fc5
d3265f5d1_slices_1-2-3-4.zip52056815ca89121fa215eef5c867ea05faf0a2c7bc33c079175ade3bb31ad214a4214458
1cf94f536_slices_1-2-3-4.zip54239426113fbb385d6bb247bf748b579398d37ad2f3db337abd588d5b5c8413f851af6b
d3265f5d1_slices_5-x-x-x.zip6393557773c7582c8f927d79262ce09bcf111ad80812f80af334f7b1e1ca032edcd93c3d
f36dde395_slices_1-2-3-4.zip46386604bb0a08939bc766de39b39ae1b18d30cfb4b5859d77b896ec370957104ad9d943
fea533069_slices_5-6-7-x.zip601300509b927fa4c892d4431d80af9a65a99f31ae5d22ded41df11868f86651866b5195
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