G2C::Electrophysiology

Akt2 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices, with no electrophysiological variables significantly affected by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Akt2 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3260 (110) 17 (5) 3750 (210) 0.00013 *** 0.26
PPF at 50ms, ampl ratio % 17 (5) 176.5 (3.7) 17 (5) 171.2 (5.8) 0.0082 ** 0.63
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 196.1 (6.4) 16 (5) 188.1 (7.3) 0.16 0.52
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 62.4 (3) 16 (5) 66.5 (4.5) 0.00055 *** 0.65
Burst 1, ave amplitude µV 17 (5) 1331 (44) 16 (5) 1426 (64) 0.00078 *** 0.47
Burst 2, ave amplitude µV 17 (5) 2091 (77) 16 (5) 2172 (156) 0.00019 *** 0.79
Burst 3, ave amplitude µV 17 (5) 2385 (91) 16 (5) 2504 (180) 8.4e-05 *** 0.73
Burst 4, ave amplitude µV 17 (5) 2497 (89) 16 (5) 2643 (181) 1e-04 *** 0.68
Burst 5, ave amplitude µV 17 (5) 2488 (83) 16 (5) 2619 (175) 0.00013 *** 0.7
Burst 6, ave amplitude µV 17 (5) 2439 (75) 16 (5) 2521 (166) 4e-04 *** 0.79
Burst 7, ave amplitude µV 17 (5) 2360 (68) 16 (5) 2440 (159) 0.00067 *** 0.78
Burst 8, ave amplitude µV 17 (5) 2300 (65) 16 (5) 2361 (154) 0.00077 *** 0.82
Burst 9, ave amplitude µV 17 (5) 2217 (63) 16 (5) 2290 (151) 0.00074 *** 0.79
Burst 10, ave amplitude µV 17 (5) 2167 (65) 16 (5) 2230 (150) 0.0016 ** 0.81
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 174.9 (3.5) 16 (5) 166.5 (5.8) 0.07 0.37
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 96 (1.3) 16 (5) 94.3 (1.6) 0.017 * 0.6
LTP vs PTP, amplitude ratio % 17 (5) 77.7 (1.2) 16 (5) 79.4 (1.8) 0.43 0.46
LTP based on amplitude % 17 (5) 169.2 (6.2) 16 (5) 183 (6.3) 0.36 0.18

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 3260 (110) 17 (5) 3750 (210) 0.00013 *** 0.26

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 17 (5) 176.5 (3.7) 17 (5) 171.2 (5.8) 0.0082 ** 0.63

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 196.1 (6.4) 16 (5) 188.1 (7.3) 0.16 0.52
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 62.4 (3) 16 (5) 66.5 (4.5) 0.00055 *** 0.65
Burst 1, ave amplitude µV 17 (5) 1331 (44) 16 (5) 1426 (64) 0.00078 *** 0.47
Burst 2, ave amplitude µV 17 (5) 2091 (77) 16 (5) 2172 (156) 0.00019 *** 0.79
Burst 3, ave amplitude µV 17 (5) 2385 (91) 16 (5) 2504 (180) 8.4e-05 *** 0.73
Burst 4, ave amplitude µV 17 (5) 2497 (89) 16 (5) 2643 (181) 1e-04 *** 0.68
Burst 5, ave amplitude µV 17 (5) 2488 (83) 16 (5) 2619 (175) 0.00013 *** 0.7
Burst 6, ave amplitude µV 17 (5) 2439 (75) 16 (5) 2521 (166) 4e-04 *** 0.79
Burst 7, ave amplitude µV 17 (5) 2360 (68) 16 (5) 2440 (159) 0.00067 *** 0.78
Burst 8, ave amplitude µV 17 (5) 2300 (65) 16 (5) 2361 (154) 0.00077 *** 0.82
Burst 9, ave amplitude µV 17 (5) 2217 (63) 16 (5) 2290 (151) 0.00074 *** 0.79
Burst 10, ave amplitude µV 17 (5) 2167 (65) 16 (5) 2230 (150) 0.0016 ** 0.81
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 174.9 (3.5) 16 (5) 166.5 (5.8) 0.07 0.37
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 96 (1.3) 16 (5) 94.3 (1.6) 0.017 * 0.6

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 17 (5) 77.7 (1.2) 16 (5) 79.4 (1.8) 0.43 0.46
LTP based on amplitude % 17 (5) 169.2 (6.2) 16 (5) 183 (6.3) 0.36 0.18

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wB73e7d8d7cWT
wA77a8b7f42WT
wA0a9c6f0b3WT
wB77a8b7f42WT
wBed363ac7eWT
mB0ad1fc0f5mutant
mB0a9c6f0b3mutant
mA08d0fbfb8mutant
mAed363ac7emutant
mA73e7d8d7cmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB0a9c6f0b3 mutant 3 37A 0a9c6f0b3_slices_3-4-5-x.zip
mB0a9c6f0b3 mutant 4 42A 0a9c6f0b3_slices_3-4-5-x.zip
mB0a9c6f0b3 mutant 5 46B 0a9c6f0b3_slices_3-4-5-x.zip
mAed363ac7e mutant 1 38A ed363ac7e_slices_1-x-2-3.zip
mAed363ac7e mutant 2 46B ed363ac7e_slices_1-x-2-3.zip
mAed363ac7e mutant 3 54B ed363ac7e_slices_1-x-2-3.zip
mA73e7d8d7c mutant 1 12A 73e7d8d7c_slices_1-x-2-3.zip
mA73e7d8d7c mutant 2 46B 73e7d8d7c_slices_1-x-2-3.zip
mA73e7d8d7c mutant 3 87B 73e7d8d7c_slices_1-x-2-3.zip
mA08d0fbfb8 mutant 1 55A 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 2 84A 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 3 38B 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 4 53B 08d0fbfb8_slices_1-2-3-4.zip
mB0ad1fc0f5 mutant 5 45A 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 6 53A 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 7 15B 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 8 52B 0ad1fc0f5_slices_5-6-7-8.zip
wA0a9c6f0b3 WT 1 46A 0a9c6f0b3_slices_1-x-x-2.zip
wA0a9c6f0b3 WT 2 53B 0a9c6f0b3_slices_1-x-x-2.zip
wBed363ac7e WT 4 47A ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 5 54A ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 6 47B ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 7 54B ed363ac7e_slices_4-5-6-7.zip
wB73e7d8d7c WT 4 56A 73e7d8d7c_slices_4-5-6-7.zip
wB73e7d8d7c WT 5 84A 73e7d8d7c_slices_4-5-6-7.zip
wB73e7d8d7c WT 6 46B 73e7d8d7c_slices_4-5-6-7.zip
wA77a8b7f42 WT 1 46A 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 2 62A 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 3 46B 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 4 61B 77a8b7f42_slices_1-2-3-4.zip
wB77a8b7f42 WT 5 58A 77a8b7f42_slices_5-6-7-8.zip
wB77a8b7f42 WT 6 87A 77a8b7f42_slices_5-6-7-8.zip
wB77a8b7f42 WT 7 46B 77a8b7f42_slices_5-6-7-8.zip
wA77a8b7f42 WT 8 42B 77a8b7f42_slices_5-6-7-8.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB0a9c6f0b3 mutant 3 37A 0a9c6f0b3_slices_3-4-5-x.zip
mB0a9c6f0b3 mutant 4 42A 0a9c6f0b3_slices_3-4-5-x.zip
mB0a9c6f0b3 mutant 5 46B 0a9c6f0b3_slices_3-4-5-x.zip
mAed363ac7e mutant 1 38A ed363ac7e_slices_1-x-2-3.zip
mAed363ac7e mutant 2 46B ed363ac7e_slices_1-x-2-3.zip
mAed363ac7e mutant 3 54B ed363ac7e_slices_1-x-2-3.zip
mA73e7d8d7c mutant 1 12A 73e7d8d7c_slices_1-x-2-3.zip
mA73e7d8d7c mutant 2 46B 73e7d8d7c_slices_1-x-2-3.zip
mA73e7d8d7c mutant 3 87B 73e7d8d7c_slices_1-x-2-3.zip
mA08d0fbfb8 mutant 1 55A 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 2 84A 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 3 38B 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 4 53B 08d0fbfb8_slices_1-2-3-4.zip
mB0ad1fc0f5 mutant 5 45A 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 6 53A 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 7 15B 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 8 52B 0ad1fc0f5_slices_5-6-7-8.zip
wA0a9c6f0b3 WT 1 46A 0a9c6f0b3_slices_1-x-x-2.zip
wA0a9c6f0b3 WT 2 53B 0a9c6f0b3_slices_1-x-x-2.zip
wBed363ac7e WT 4 47A ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 5 54A ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 6 47B ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 7 54B ed363ac7e_slices_4-5-6-7.zip
wB73e7d8d7c WT 4 56A 73e7d8d7c_slices_4-5-6-7.zip
wB73e7d8d7c WT 5 84A 73e7d8d7c_slices_4-5-6-7.zip
wB73e7d8d7c WT 6 46B 73e7d8d7c_slices_4-5-6-7.zip
wA77a8b7f42 WT 1 46A 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 2 62A 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 3 46B 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 4 61B 77a8b7f42_slices_1-2-3-4.zip
wB77a8b7f42 WT 5 58A 77a8b7f42_slices_5-6-7-8.zip
wB77a8b7f42 WT 6 87A 77a8b7f42_slices_5-6-7-8.zip
wB77a8b7f42 WT 7 46B 77a8b7f42_slices_5-6-7-8.zip
wA77a8b7f42 WT 8 42B 77a8b7f42_slices_5-6-7-8.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB0a9c6f0b3 mutant 3 37A 0a9c6f0b3_slices_3-4-5-x.zip
mB0a9c6f0b3 mutant 4 42A 0a9c6f0b3_slices_3-4-5-x.zip
mB0a9c6f0b3 mutant 5 46B 0a9c6f0b3_slices_3-4-5-x.zip
mAed363ac7e mutant 1 38A ed363ac7e_slices_1-x-2-3.zip
mAed363ac7e mutant 2 46B ed363ac7e_slices_1-x-2-3.zip
mAed363ac7e mutant 3 54B ed363ac7e_slices_1-x-2-3.zip
mA73e7d8d7c mutant 1 12A 73e7d8d7c_slices_1-x-2-3.zip
mA73e7d8d7c mutant 2 46B 73e7d8d7c_slices_1-x-2-3.zip
mA73e7d8d7c mutant 3 87B 73e7d8d7c_slices_1-x-2-3.zip
mA08d0fbfb8 mutant 1 55A 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 2 84A 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 3 38B 08d0fbfb8_slices_1-2-3-4.zip
mA08d0fbfb8 mutant 4 53B 08d0fbfb8_slices_1-2-3-4.zip
mB0ad1fc0f5 mutant 5 45A 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 6 53A 0ad1fc0f5_slices_5-6-7-8.zip
mB0ad1fc0f5 mutant 8 52B 0ad1fc0f5_slices_5-6-7-8.zip
wA0a9c6f0b3 WT 1 46A 0a9c6f0b3_slices_1-x-x-2.zip
wA0a9c6f0b3 WT 2 53B 0a9c6f0b3_slices_1-x-x-2.zip
wBed363ac7e WT 4 47A ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 5 54A ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 6 47B ed363ac7e_slices_4-5-6-7.zip
wBed363ac7e WT 7 54B ed363ac7e_slices_4-5-6-7.zip
wB73e7d8d7c WT 4 56A 73e7d8d7c_slices_4-5-6-7.zip
wB73e7d8d7c WT 5 84A 73e7d8d7c_slices_4-5-6-7.zip
wB73e7d8d7c WT 6 46B 73e7d8d7c_slices_4-5-6-7.zip
wA77a8b7f42 WT 1 46A 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 2 62A 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 3 46B 77a8b7f42_slices_1-2-3-4.zip
wA77a8b7f42 WT 4 61B 77a8b7f42_slices_1-2-3-4.zip
wB77a8b7f42 WT 5 58A 77a8b7f42_slices_5-6-7-8.zip
wB77a8b7f42 WT 6 87A 77a8b7f42_slices_5-6-7-8.zip
wB77a8b7f42 WT 7 46B 77a8b7f42_slices_5-6-7-8.zip
wA77a8b7f42 WT 8 42B 77a8b7f42_slices_5-6-7-8.zip

Data Files

File NameSizeChecksum (SHA256)
0a9c6f0b3_slices_3-4-5-x.zip45683753761d5176f41c1554012b903d685ae35d59992bcc619e64f5b113eb30fc16d6bd
77a8b7f42_slices_1-2-3-4.zip30738725343a81b7e5f9b45d7c8d71a0b716c0d18ffdb58f455180326c68572aed0213de
73e7d8d7c_slices_4-5-6-7.zip50005665b1407463df15c725ef129d436662f7b75770351542054f5b4a3d3eef820310c1
77a8b7f42_slices_5-6-7-8.zip483234735b20cf0836beaf5a611f635bdc51af56ad636da705ed0a5763e50a8c13862a9a
08d0fbfb8_slices_1-2-3-4.zip34868863cfb0c379d5b12c7e4ac03d0e3284fdd15f0355945e28966d74c4fa6d86241101
0ad1fc0f5_slices_5-6-7-8.zip46717487d813ec23547d178f5c36697187bb38d6fa5ceff7090c45ed43ce12a476bb4cc7
ed363ac7e_slices_1-x-2-3.zip36708360899808dd074d70b18068c782f7dd734d8c7851a4a6fb08aa42d7fff6d3d1054a
0a9c6f0b3_slices_1-x-x-2.zip253771885d8d4722b53bfe2f8182a444812607009a83f749102b16ae0fd4c747c95049d7
ed363ac7e_slices_4-5-6-7.zip533938431adb7f5b0658f6fe42cc563f7ff6e07a614630e82a1c450345ef5f70813b7803
73e7d8d7c_slices_1-x-2-3.zip406097408b6cc2025ed2dc844a2deb0788d5af1f816a1b2d3c907aab7d852d97d6fd8c95
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