G2C::Electrophysiology

SAP97 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices. With heterozygous genotype and only one electrophysiological variable significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the SAP97 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 2890 (170) 14 (6) 2860 (130) 0.37 0.89
PPF at 50ms, ampl ratio % 17 (5) 180.2 (5.9) 14 (6) 176.2 (8.5) 0.45 0.71
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 200 (12) 14 (6) 198 (13) 0.58 0.93
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 69.9 (3.9) 14 (6) 70.8 (4.3) 0.37 0.89
Burst 1, ave amplitude µV 17 (5) 1269 (59) 14 (6) 1222 (52) 0.83 0.47
Burst 2, ave amplitude µV 17 (5) 1888 (126) 14 (6) 1776 (93) 0.55 0.49
Burst 3, ave amplitude µV 17 (5) 2190 (150) 14 (6) 2070 (120) 0.64 0.5
Burst 4, ave amplitude µV 17 (5) 2340 (160) 14 (6) 2240 (130) 0.67 0.6
Burst 5, ave amplitude µV 17 (5) 2320 (160) 14 (6) 2260 (130) 0.7 0.74
Burst 6, ave amplitude µV 17 (5) 2290 (150) 14 (6) 2240 (130) 0.72 0.79
Burst 7, ave amplitude µV 17 (5) 2230 (150) 14 (6) 2180 (130) 0.72 0.78
Burst 8, ave amplitude µV 17 (5) 2160 (150) 14 (6) 2130 (130) 0.7 0.89
Burst 9, ave amplitude µV 17 (5) 2090 (150) 14 (6) 2080 (130) 0.74 0.96
Burst 10, ave amplitude µV 17 (5) 2050 (150) 14 (6) 2040 (120) 0.75 0.93
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 168.1 (4.9) 14 (6) 168.8 (8.7) 0.31 0.95
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 97.5 (1.1) 14 (6) 101.7 (1.8) 0.93 0.016 *
LTP vs PTP, amplitude ratio % 17 (5) 79.4 (1.4) 14 (6) 74.5 (1.5) 0.051 0.11
LTP based on amplitude % 17 (5) 175.1 (6.9) 14 (6) 176.8 (10) 0.71 0.87

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 17 (5) 2890 (170) 14 (6) 2860 (130) 0.37 0.89

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 17 (5) 180.2 (5.9) 14 (6) 176.2 (8.5) 0.45 0.71

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 17 (5) 200 (12) 14 (6) 198 (13) 0.58 0.93
Burst 1, EPSP3 depr, ampl ratio % 17 (5) 69.9 (3.9) 14 (6) 70.8 (4.3) 0.37 0.89
Burst 1, ave amplitude µV 17 (5) 1269 (59) 14 (6) 1222 (52) 0.83 0.47
Burst 2, ave amplitude µV 17 (5) 1888 (126) 14 (6) 1776 (93) 0.55 0.49
Burst 3, ave amplitude µV 17 (5) 2190 (150) 14 (6) 2070 (120) 0.64 0.5
Burst 4, ave amplitude µV 17 (5) 2340 (160) 14 (6) 2240 (130) 0.67 0.6
Burst 5, ave amplitude µV 17 (5) 2320 (160) 14 (6) 2260 (130) 0.7 0.74
Burst 6, ave amplitude µV 17 (5) 2290 (150) 14 (6) 2240 (130) 0.72 0.79
Burst 7, ave amplitude µV 17 (5) 2230 (150) 14 (6) 2180 (130) 0.72 0.78
Burst 8, ave amplitude µV 17 (5) 2160 (150) 14 (6) 2130 (130) 0.7 0.89
Burst 9, ave amplitude µV 17 (5) 2090 (150) 14 (6) 2080 (130) 0.74 0.96
Burst 10, ave amplitude µV 17 (5) 2050 (150) 14 (6) 2040 (120) 0.75 0.93
Burst 2-4 ave amplitude vs Burst 1 % 17 (5) 168.1 (4.9) 14 (6) 168.8 (8.7) 0.31 0.95
Burst 8-10 ave amplitude vs Burst 2-4 % 17 (5) 97.5 (1.1) 14 (6) 101.7 (1.8) 0.93 0.016 *

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 17 (5) 79.4 (1.4) 14 (6) 74.5 (1.5) 0.051 0.11
LTP based on amplitude % 17 (5) 175.1 (6.9) 14 (6) 176.8 (10) 0.71 0.87

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant6

 

Individual Mice

Mouse IDGenotype
wAa94cd9b6eWT
wAa2d622570WT
wBc89f97260WT
wBa22f6d6a1WT
wA7e3802dc6WT
mA1258a4df1mutant
mB90c508958mutant
mBa2d622570mutant
mBc35dfa035mutant
mA90c508958mutant
mAc89f97260mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA90c508958 mutant 1 46A 90c508958_slices_1-2-3-x.zip
mA90c508958 mutant 2 62A 90c508958_slices_1-2-3-x.zip
mB90c508958 mutant 3 46B 90c508958_slices_1-2-3-x.zip
mB90c508958 mutant 4 62A 90c508958_slices_4-x-x-x.zip
mAc89f97260 mutant 1 12A c89f97260_slices_1-x-2-3.zip
mAc89f97260 mutant 2 24B c89f97260_slices_1-x-2-3.zip
mAc89f97260 mutant 3 54B c89f97260_slices_1-x-2-3.zip
mA1258a4df1 mutant 1 12A 1258a4df1_slices_1-2-x-3.zip
mA1258a4df1 mutant 2 52A 1258a4df1_slices_1-2-x-3.zip
mA1258a4df1 mutant 3 43B 1258a4df1_slices_1-2-x-3.zip
mBa2d622570 mutant 6 41A a2d622570_slices_5-6-7-x.zip
mBa2d622570 mutant 7 47B a2d622570_slices_5-6-7-x.zip
mBc35dfa035 mutant 4 67A c35dfa035_slices_4--x-5-x.zip
mBc35dfa035 mutant 5 47B c35dfa035_slices_4--x-5-x.zip
wBc89f97260 WT 4 44A c89f97260_slices_4-5-x-6.zip
wBc89f97260 WT 5 61A c89f97260_slices_4-5-x-6.zip
wBc89f97260 WT 6 61B c89f97260_slices_4-5-x-6.zip
wAa2d622570 WT 1 24A a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 2 53A a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 3 46B a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 4 53B a2d622570_slices_1-2-3-4.zip
wAa94cd9b6e WT 1 46A a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 2 73A a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 3 12B a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 4 62B a94cd9b6e_slices_1-2-3-4.zip
wA7e3802dc6 WT 1 87A 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 2 61A 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 3 12B 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 4 61B 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 5 47A 7e3802dc6_slices_5-6-7-8.zip
wBa22f6d6a1 WT 4 38A a22f6d6a1_slices_4-x-x-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA90c508958 mutant 1 46A 90c508958_slices_1-2-3-x.zip
mA90c508958 mutant 2 62A 90c508958_slices_1-2-3-x.zip
mB90c508958 mutant 3 46B 90c508958_slices_1-2-3-x.zip
mB90c508958 mutant 4 62A 90c508958_slices_4-x-x-x.zip
mAc89f97260 mutant 1 12A c89f97260_slices_1-x-2-3.zip
mAc89f97260 mutant 2 24B c89f97260_slices_1-x-2-3.zip
mAc89f97260 mutant 3 54B c89f97260_slices_1-x-2-3.zip
mA1258a4df1 mutant 1 12A 1258a4df1_slices_1-2-x-3.zip
mA1258a4df1 mutant 2 52A 1258a4df1_slices_1-2-x-3.zip
mA1258a4df1 mutant 3 43B 1258a4df1_slices_1-2-x-3.zip
mBa2d622570 mutant 6 41A a2d622570_slices_5-6-7-x.zip
mBa2d622570 mutant 7 47B a2d622570_slices_5-6-7-x.zip
mBc35dfa035 mutant 4 67A c35dfa035_slices_4--x-5-x.zip
mBc35dfa035 mutant 5 47B c35dfa035_slices_4--x-5-x.zip
wBc89f97260 WT 4 44A c89f97260_slices_4-5-x-6.zip
wBc89f97260 WT 5 61A c89f97260_slices_4-5-x-6.zip
wBc89f97260 WT 6 61B c89f97260_slices_4-5-x-6.zip
wAa2d622570 WT 1 24A a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 2 53A a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 3 46B a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 4 53B a2d622570_slices_1-2-3-4.zip
wAa94cd9b6e WT 1 46A a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 2 73A a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 3 12B a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 4 62B a94cd9b6e_slices_1-2-3-4.zip
wA7e3802dc6 WT 1 87A 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 2 61A 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 3 12B 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 4 61B 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 5 47A 7e3802dc6_slices_5-6-7-8.zip
wBa22f6d6a1 WT 4 38A a22f6d6a1_slices_4-x-x-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mA90c508958 mutant 1 46A 90c508958_slices_1-2-3-x.zip
mA90c508958 mutant 2 62A 90c508958_slices_1-2-3-x.zip
mB90c508958 mutant 3 46B 90c508958_slices_1-2-3-x.zip
mB90c508958 mutant 4 62A 90c508958_slices_4-x-x-x.zip
mAc89f97260 mutant 1 12A c89f97260_slices_1-x-2-3.zip
mAc89f97260 mutant 2 24B c89f97260_slices_1-x-2-3.zip
mAc89f97260 mutant 3 54B c89f97260_slices_1-x-2-3.zip
mA1258a4df1 mutant 1 12A 1258a4df1_slices_1-2-x-3.zip
mA1258a4df1 mutant 2 52A 1258a4df1_slices_1-2-x-3.zip
mA1258a4df1 mutant 3 43B 1258a4df1_slices_1-2-x-3.zip
mBa2d622570 mutant 6 41A a2d622570_slices_5-6-7-x.zip
mBa2d622570 mutant 7 47B a2d622570_slices_5-6-7-x.zip
mBc35dfa035 mutant 4 67A c35dfa035_slices_4--x-5-x.zip
mBc35dfa035 mutant 5 47B c35dfa035_slices_4--x-5-x.zip
wBc89f97260 WT 4 44A c89f97260_slices_4-5-x-6.zip
wBc89f97260 WT 5 61A c89f97260_slices_4-5-x-6.zip
wBc89f97260 WT 6 61B c89f97260_slices_4-5-x-6.zip
wAa2d622570 WT 1 24A a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 2 53A a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 3 46B a2d622570_slices_1-2-3-4.zip
wAa2d622570 WT 4 53B a2d622570_slices_1-2-3-4.zip
wAa94cd9b6e WT 1 46A a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 2 73A a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 3 12B a94cd9b6e_slices_1-2-3-4.zip
wAa94cd9b6e WT 4 62B a94cd9b6e_slices_1-2-3-4.zip
wA7e3802dc6 WT 1 87A 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 2 61A 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 3 12B 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 4 61B 7e3802dc6_slices_1-2-3-4.zip
wA7e3802dc6 WT 5 47A 7e3802dc6_slices_5-6-7-8.zip
wBa22f6d6a1 WT 4 38A a22f6d6a1_slices_4-x-x-x.zip

Data Files

File NameSizeChecksum (SHA256)
a22f6d6a1_slices_4-x-x-x.zip4381760421ff44b80820c126f23b1b9eaf4b181e36bca1a39eadc5540934ec801943487e
7e3802dc6_slices_1-2-3-4.zip378137787daaba7d91109ee3817936756f97469e2ff0445ea76e6b5b1c40c77e48b91970
90c508958_slices_4-x-x-x.zip4911884677dbd9927f653675826e2b5f4662131d4f5c920897f1f2750a479a04b8df335e
a94cd9b6e_slices_1-2-3-4.zip54775648c57c828830d1a8c45d5d9745772e336b12d84b86aef5cdf43a6a6b343e8c0daa
c89f97260_slices_4-5-x-6.zip551110152986737445bbda62b493f7df012e8b7e037a58ddade0faea0ba1e858cb320696
c89f97260_slices_1-x-2-3.zip3126417230bbfea233a87981c82f8ef015c097e63ace0b92bfea0381ea4cae2695396c72
7e3802dc6_slices_5-6-7-8.zip63156509db1a49fb29597459d5e48dfe02f4af34bf624db334fac794e8e12d62de5ef7e6
a2d622570_slices_5-6-7-x.zip46004210d0c4c7d53a50808138970354935b4aada584d1d692cf327c7e8eaacdc0dd83fa
90c508958_slices_1-2-3-x.zip370743974d317161b3358e40409a48b02fdd85a4fd6d74d46ef19c6349780d1245b5f47b
1258a4df1_slices_1-2-x-3.zip45814504ff5d202f14d1db146b4aae8ed170539438015410db8eb05678041d866a4f4fde
c35dfa035_slices_4--x-5-x.zip45145548935d290660593ea1b1cbb0a977389e02a17bd952f9dc53c808a5cebdab4d373e
a2d622570_slices_1-2-3-4.zip336456290a82ffa59aba24d1391f5f66a6c52a89a64a9d80ef32b378862ce7c3f4f7ac5f
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