G2C::Electrophysiology

Git1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed medium overall electrophysiological difference from wildtype slices, with four of nine variables significantly impacted by this mutation. In the paired pulse facilitation experiment, PPF at 50ms ampl ratio was significantly decreased in mutants. In the theta burst stimulation experiment, three electrophysiological variables of five were significantly impacted in mutants. Basal synaptic transmission and long term potentiation experiments were unaffected. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the Git1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 15 (6) 2790 (140) 21 (7) 2880 (130) 0.25 0.7
PPF at 50ms, ampl ratio % 15 (6) 180 (5.6) 21 (7) 155.1 (3.8) 0.58 0.0039 **
Burst 1, PPF at 10ms, ampl ratio % 15 (6) 191.1 (10.3) 18 (6) 157.7 (6.3) 0.14 0.04 *
Burst 1, EPSP3 depr, ampl ratio % 15 (6) 66 (2.8) 18 (6) 64.6 (2.7) 0.21 0.76
Burst 1, ave amplitude µV 15 (6) 1252 (41) 18 (6) 1237 (40) 0.51 0.8
Burst 2, ave amplitude µV 15 (6) 2009 (114) 18 (6) 1675 (66) 0.33 0.036 *
Burst 3, ave amplitude µV 15 (6) 2223 (132) 18 (6) 1766 (77) 0.27 0.02 *
Burst 4, ave amplitude µV 15 (6) 2290 (128) 18 (6) 1870 (74) 0.25 0.027 *
Burst 5, ave amplitude µV 15 (6) 2277 (133) 18 (6) 1902 (77) 0.14 0.062
Burst 6, ave amplitude µV 15 (6) 2218 (129) 18 (6) 1885 (78) 0.082 0.1
Burst 7, ave amplitude µV 15 (6) 2150 (129) 18 (6) 1854 (76) 0.053 0.15
Burst 8, ave amplitude µV 15 (6) 2091 (130) 18 (6) 1802 (77) 0.057 0.15
Burst 9, ave amplitude µV 15 (6) 2026 (129) 18 (6) 1755 (75) 0.035 * 0.19
Burst 10, ave amplitude µV 15 (6) 1984 (131) 18 (6) 1733 (78) 0.042 * 0.22
Burst 2-4 ave amplitude vs Burst 1 % 15 (6) 173.6 (6.6) 18 (6) 141.8 (2.9) 0.23 0.0023 **
Burst 8-10 ave amplitude vs Burst 2-4 % 15 (6) 93.2 (1.2) 18 (6) 100.5 (2.1) 0.077 0.048 *
LTP vs PTP, amplitude ratio % 15 (6) 78.2 (1) 18 (6) 80.2 (1.4) 0.92 0.15
LTP based on amplitude % 15 (6) 173.2 (7.8) 18 (6) 162.5 (4.9) 0.18 0.34

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 15 (6) 2790 (140) 21 (7) 2880 (130) 0.25 0.7

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 15 (6) 180 (5.6) 21 (7) 155.1 (3.8) 0.58 0.0039 **

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 15 (6) 191.1 (10.3) 18 (6) 157.7 (6.3) 0.14 0.04 *
Burst 1, EPSP3 depr, ampl ratio % 15 (6) 66 (2.8) 18 (6) 64.6 (2.7) 0.21 0.76
Burst 1, ave amplitude µV 15 (6) 1252 (41) 18 (6) 1237 (40) 0.51 0.8
Burst 2, ave amplitude µV 15 (6) 2009 (114) 18 (6) 1675 (66) 0.33 0.036 *
Burst 3, ave amplitude µV 15 (6) 2223 (132) 18 (6) 1766 (77) 0.27 0.02 *
Burst 4, ave amplitude µV 15 (6) 2290 (128) 18 (6) 1870 (74) 0.25 0.027 *
Burst 5, ave amplitude µV 15 (6) 2277 (133) 18 (6) 1902 (77) 0.14 0.062
Burst 6, ave amplitude µV 15 (6) 2218 (129) 18 (6) 1885 (78) 0.082 0.1
Burst 7, ave amplitude µV 15 (6) 2150 (129) 18 (6) 1854 (76) 0.053 0.15
Burst 8, ave amplitude µV 15 (6) 2091 (130) 18 (6) 1802 (77) 0.057 0.15
Burst 9, ave amplitude µV 15 (6) 2026 (129) 18 (6) 1755 (75) 0.035 * 0.19
Burst 10, ave amplitude µV 15 (6) 1984 (131) 18 (6) 1733 (78) 0.042 * 0.22
Burst 2-4 ave amplitude vs Burst 1 % 15 (6) 173.6 (6.6) 18 (6) 141.8 (2.9) 0.23 0.0023 **
Burst 8-10 ave amplitude vs Burst 2-4 % 15 (6) 93.2 (1.2) 18 (6) 100.5 (2.1) 0.077 0.048 *

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 15 (6) 78.2 (1) 18 (6) 80.2 (1.4) 0.92 0.15
LTP based on amplitude % 15 (6) 173.2 (7.8) 18 (6) 162.5 (4.9) 0.18 0.34

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT6
mutant7

 

Individual Mice

Mouse IDGenotype
wA324f9ab7eWT
wAaccea82b7WT
wA6ab88826fWT
wAc16eaef45WT
wBd2028f7c2WT
wB6ab88826fWT
mAd76472457mutant
mB1d8c77d6cmutant
mAaccea82b7mutant
mBd76472457mutant
mBc16eaef45mutant
mA6ab88826fmutant
mB324f9ab7emutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB1d8c77d6c mutant 6 52A 1d8c77d6c_slices_5-6-7-8.zip
mB1d8c77d6c mutant 7 47B 1d8c77d6c_slices_5-6-7-8.zip
mB1d8c77d6c mutant 8 53B 1d8c77d6c_slices_5-6-7-8.zip
mBc16eaef45 mutant 4 58A c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 5 54A c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 6 57B c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 7 43B c16eaef45_slices_4-5-6-7.zip
mB324f9ab7e mutant 5 12A 324f9ab7e_slices_5-6-7-x.zip
mB324f9ab7e mutant 6 44A 324f9ab7e_slices_5-6-7-x.zip
mB324f9ab7e mutant 7 58B 324f9ab7e_slices_5-6-7-x.zip
mAaccea82b7 mutant 1 12A accea82b7_slices_1-2-x-3.zip
mAaccea82b7 mutant 2 52A accea82b7_slices_1-2-x-3.zip
mA6ab88826f mutant 1 46A 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 2 42A 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 3 46B 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 4 87B 6ab88826f_slices_1-2-3-4.zip
mAd76472457 mutant 1 52A d76472457_slices_x-1-2-3.zip
mAd76472457 mutant 2 12B d76472457_slices_x-1-2-3.zip
mAd76472457 mutant 3 52B d76472457_slices_x-1-2-3.zip
mBd76472457 mutant 4 46A d76472457_slices_4-5-x-x.zip
mBd76472457 mutant 5 87A d76472457_slices_4-5-x-x.zip
wAc16eaef45 WT 1 46A c16eaef45_slices_1-2-3-x.zip
wAc16eaef45 WT 2 43A c16eaef45_slices_1-2-3-x.zip
wAc16eaef45 WT 3 38B c16eaef45_slices_1-2-3-x.zip
wA324f9ab7e WT 1 55A 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 2 54A 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 3 55B 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 4 43B 324f9ab7e_slices_1-2-3-4.zip
wAaccea82b7 WT 4 24A accea82b7_slices_4-x-x-x.zip
wA6ab88826f WT 5 24A 6ab88826f_slice_5-x-6-7.zip
wB6ab88826f WT 6 56B 6ab88826f_slice_5-x-6-7.zip
wB6ab88826f WT 7 41B 6ab88826f_slice_5-x-6-7.zip
wBd2028f7c2 WT 5 55A d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 6 54A d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 7 57B d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 8 62B d2028f7c2_slices_5-6-7-8.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB1d8c77d6c mutant 6 52A 1d8c77d6c_slices_5-6-7-8.zip
mB1d8c77d6c mutant 7 47B 1d8c77d6c_slices_5-6-7-8.zip
mB1d8c77d6c mutant 8 53B 1d8c77d6c_slices_5-6-7-8.zip
mBc16eaef45 mutant 4 58A c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 5 54A c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 6 57B c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 7 43B c16eaef45_slices_4-5-6-7.zip
mB324f9ab7e mutant 5 12A 324f9ab7e_slices_5-6-7-x.zip
mB324f9ab7e mutant 6 44A 324f9ab7e_slices_5-6-7-x.zip
mB324f9ab7e mutant 7 58B 324f9ab7e_slices_5-6-7-x.zip
mAaccea82b7 mutant 1 12A accea82b7_slices_1-2-x-3.zip
mAaccea82b7 mutant 2 52A accea82b7_slices_1-2-x-3.zip
mA6ab88826f mutant 1 46A 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 2 42A 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 3 46B 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 4 87B 6ab88826f_slices_1-2-3-4.zip
mAd76472457 mutant 1 52A d76472457_slices_x-1-2-3.zip
mAd76472457 mutant 2 12B d76472457_slices_x-1-2-3.zip
mAd76472457 mutant 3 52B d76472457_slices_x-1-2-3.zip
mBd76472457 mutant 4 46A d76472457_slices_4-5-x-x.zip
mBd76472457 mutant 5 87A d76472457_slices_4-5-x-x.zip
wAc16eaef45 WT 1 46A c16eaef45_slices_1-2-3-x.zip
wAc16eaef45 WT 2 43A c16eaef45_slices_1-2-3-x.zip
wAc16eaef45 WT 3 38B c16eaef45_slices_1-2-3-x.zip
wA324f9ab7e WT 1 55A 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 2 54A 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 3 55B 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 4 43B 324f9ab7e_slices_1-2-3-4.zip
wAaccea82b7 WT 4 24A accea82b7_slices_4-x-x-x.zip
wA6ab88826f WT 5 24A 6ab88826f_slice_5-x-6-7.zip
wB6ab88826f WT 6 56B 6ab88826f_slice_5-x-6-7.zip
wB6ab88826f WT 7 41B 6ab88826f_slice_5-x-6-7.zip
wBd2028f7c2 WT 5 55A d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 6 54A d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 7 57B d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 8 62B d2028f7c2_slices_5-6-7-8.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mB1d8c77d6c mutant 6 52A 1d8c77d6c_slices_5-6-7-8.zip
mB1d8c77d6c mutant 7 47B 1d8c77d6c_slices_5-6-7-8.zip
mB1d8c77d6c mutant 8 53B 1d8c77d6c_slices_5-6-7-8.zip
mBc16eaef45 mutant 4 58A c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 5 54A c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 6 57B c16eaef45_slices_4-5-6-7.zip
mBc16eaef45 mutant 7 43B c16eaef45_slices_4-5-6-7.zip
mAaccea82b7 mutant 1 12A accea82b7_slices_1-2-x-3.zip
mAaccea82b7 mutant 2 52A accea82b7_slices_1-2-x-3.zip
mA6ab88826f mutant 1 46A 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 2 42A 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 3 46B 6ab88826f_slices_1-2-3-4.zip
mA6ab88826f mutant 4 87B 6ab88826f_slices_1-2-3-4.zip
mAd76472457 mutant 1 52A d76472457_slices_x-1-2-3.zip
mAd76472457 mutant 2 12B d76472457_slices_x-1-2-3.zip
mAd76472457 mutant 3 52B d76472457_slices_x-1-2-3.zip
mBd76472457 mutant 4 46A d76472457_slices_4-5-x-x.zip
mBd76472457 mutant 5 87A d76472457_slices_4-5-x-x.zip
wAc16eaef45 WT 1 46A c16eaef45_slices_1-2-3-x.zip
wAc16eaef45 WT 2 43A c16eaef45_slices_1-2-3-x.zip
wAc16eaef45 WT 3 38B c16eaef45_slices_1-2-3-x.zip
wA324f9ab7e WT 1 55A 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 2 54A 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 3 55B 324f9ab7e_slices_1-2-3-4.zip
wA324f9ab7e WT 4 43B 324f9ab7e_slices_1-2-3-4.zip
wAaccea82b7 WT 4 24A accea82b7_slices_4-x-x-x.zip
wA6ab88826f WT 5 24A 6ab88826f_slice_5-x-6-7.zip
wB6ab88826f WT 6 56B 6ab88826f_slice_5-x-6-7.zip
wB6ab88826f WT 7 41B 6ab88826f_slice_5-x-6-7.zip
wBd2028f7c2 WT 5 55A d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 6 54A d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 7 57B d2028f7c2_slices_5-6-7-8.zip
wBd2028f7c2 WT 8 62B d2028f7c2_slices_5-6-7-8.zip

Data Files

File NameSizeChecksum (SHA256)
d76472457_slices_x-1-2-3.zip3343424505074adf48690c80c39393b3ed9c06948778f14b8b57c6bafc815d62fa76d8cb
324f9ab7e_slices_5-6-7-x.zip7324188e3123217b775b456c63869d2cd778e7012bd1473bc4e4c0267bb247bf2f9e2b9
d76472457_slices_4-5-x-x.zip47106975dfe1b63c589eca854e4ba54d69ac4ad41359ea89d2052c7ab7cc6be803b6a372
6ab88826f_slice_5-x-6-7.zip452237706d38867cc169bb7040152655a0590484c8b1f10383434f4a0b4f0dd6d18ae62f
324f9ab7e_slices_1-2-3-4.zip36155702a16c00638f8114d6d991c19a591f7448ff80bc5af439037f4f1a8cd8c51060df
6ab88826f_slices_1-2-3-4.zip331308547a1a30b958ad1f5defffb9c8b6bd7d7fffaed903b9f2318595a92dbd6c4a3f53
c16eaef45_slices_4-5-6-7.zip43481421c3b23dbee66438bb230e2201828335de19af7784814274310adda9e0891629b5
accea82b7_slices_4-x-x-x.zip424668790eb40b1bf3b35863b57672ccaae0912824a63d163aca0f170e22783961ce4658
d2028f7c2_slices_5-6-7-8.zip471705325d33b50f5e2db17dce3156de58c9be82b339c70ba5af3f04f458b2c27bf742a9
c16eaef45_slices_1-2-3-x.zip33124574b75de9ac9f58ff2c27a0abfccf136b5084dbfba8fc43eea8c1b874ae1dbcc2f1
1d8c77d6c_slices_5-6-7-8.zip43987442e84bbf56810c28072e7d48a114a31c1840137893b290438d3be74332c3044e42
accea82b7_slices_1-2-x-3.zip366167515bcbac38fc555819fb038fc85454482f40ef03fd7cb70b03a944a864c1f426e6
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