G2C::Electrophysiology

nArgBP2 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with no electrophysiological variables significantly affected by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the nArgBP2 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 18 (6) 3540 (130) 16 (6) 3370 (110) 0.11 0.44
PPF at 50ms, ampl ratio % 18 (6) 178.9 (3.3) 16 (6) 185.4 (4.3) 0.31 0.3
Burst 1, PPF at 10ms, ampl ratio % 18 (6) 207.4 (5.5) 16 (6) 218.6 (6.4) 0.52 0.21
Burst 1, EPSP3 depr, ampl ratio % 18 (6) 62.3 (2) 16 (6) 59 (3.3) 0.00016 *** 0.6
Burst 1, ave amplitude µV 18 (6) 1450 (47) 16 (6) 1363 (63) 0.065 0.41
Burst 2, ave amplitude µV 18 (6) 2331 (102) 16 (6) 2162 (94) 0.0058 ** 0.44
Burst 3, ave amplitude µV 18 (6) 2630 (110) 16 (6) 2480 (110) 0.0026 ** 0.56
Burst 4, ave amplitude µV 18 (6) 2700 (110) 16 (6) 2560 (110) 0.00099 *** 0.58
Burst 5, ave amplitude µV 18 (6) 2640 (100) 16 (6) 2490 (100) 0.00062 *** 0.53
Burst 6, ave amplitude µV 18 (6) 2568 (96) 16 (6) 2416 (103) 0.00088 *** 0.51
Burst 7, ave amplitude µV 18 (6) 2485 (96) 16 (6) 2315 (100) 0.00074 *** 0.46
Burst 8, ave amplitude µV 18 (6) 2400 (94) 16 (6) 2253 (101) 0.00034 *** 0.52
Burst 9, ave amplitude µV 18 (6) 2335 (92) 16 (6) 2192 (101) 0.00023 *** 0.53
Burst 10, ave amplitude µV 18 (6) 2276 (89) 16 (6) 2146 (102) 0.00012 *** 0.57
Burst 2-4 ave amplitude vs Burst 1 % 18 (6) 175.5 (4.2) 16 (6) 178.7 (5.4) 0.13 0.71
Burst 8-10 ave amplitude vs Burst 2-4 % 18 (6) 91.7 (1.6) 16 (6) 91.3 (1.8) 0.04 * 0.91
LTP vs PTP, amplitude ratio % 18 (6) 81.6 (1.2) 16 (6) 81.3 (1.4) 0.44 0.86
LTP based on amplitude % 18 (6) 185.1 (3.8) 16 (6) 185.8 (5.1) 0.017 * 0.94

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 18 (6) 3540 (130) 16 (6) 3370 (110) 0.11 0.44

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 18 (6) 178.9 (3.3) 16 (6) 185.4 (4.3) 0.31 0.3

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 18 (6) 207.4 (5.5) 16 (6) 218.6 (6.4) 0.52 0.21
Burst 1, EPSP3 depr, ampl ratio % 18 (6) 62.3 (2) 16 (6) 59 (3.3) 0.00016 *** 0.6
Burst 1, ave amplitude µV 18 (6) 1450 (47) 16 (6) 1363 (63) 0.065 0.41
Burst 2, ave amplitude µV 18 (6) 2331 (102) 16 (6) 2162 (94) 0.0058 ** 0.44
Burst 3, ave amplitude µV 18 (6) 2630 (110) 16 (6) 2480 (110) 0.0026 ** 0.56
Burst 4, ave amplitude µV 18 (6) 2700 (110) 16 (6) 2560 (110) 0.00099 *** 0.58
Burst 5, ave amplitude µV 18 (6) 2640 (100) 16 (6) 2490 (100) 0.00062 *** 0.53
Burst 6, ave amplitude µV 18 (6) 2568 (96) 16 (6) 2416 (103) 0.00088 *** 0.51
Burst 7, ave amplitude µV 18 (6) 2485 (96) 16 (6) 2315 (100) 0.00074 *** 0.46
Burst 8, ave amplitude µV 18 (6) 2400 (94) 16 (6) 2253 (101) 0.00034 *** 0.52
Burst 9, ave amplitude µV 18 (6) 2335 (92) 16 (6) 2192 (101) 0.00023 *** 0.53
Burst 10, ave amplitude µV 18 (6) 2276 (89) 16 (6) 2146 (102) 0.00012 *** 0.57
Burst 2-4 ave amplitude vs Burst 1 % 18 (6) 175.5 (4.2) 16 (6) 178.7 (5.4) 0.13 0.71
Burst 8-10 ave amplitude vs Burst 2-4 % 18 (6) 91.7 (1.6) 16 (6) 91.3 (1.8) 0.04 * 0.91

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 18 (6) 81.6 (1.2) 16 (6) 81.3 (1.4) 0.44 0.86
LTP based on amplitude % 18 (6) 185.1 (3.8) 16 (6) 185.8 (5.1) 0.017 * 0.94

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT6
mutant6

 

Individual Mice

Mouse IDGenotype
wBc27f87c73WT
wA5a57423e6WT
wAcf610a6f4WT
wA5e5a8dc5bWT
wB85f568a1dWT
wA20809fa60WT
mB20809fa60mutant
mBcf610a6f4mutant
mAc27f87c73mutant
mB5e5a8dc5bmutant
mA85f568a1dmutant
mB342791472mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAc27f87c73 mutant 1 55A c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 2 52A c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 3 12B c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 4 46A c27f87c73_slices_4-5-6-7.zip
mB342791472 mutant 5 43A 342791472_slices_4-5-6-7.zip
mB342791472 mutant 6 46B 342791472_slices_4-5-6-7.zip
mB342791472 mutant 7 52B 342791472_slices_4-5-6-7.zip
mBcf610a6f4 mutant 4 54A cf610a6f4_slices_3-4-5-6.zip
mBcf610a6f4 mutant 5 46B cf610a6f4_slices_3-4-5-6.zip
mB20809fa60 mutant 6 55B 20809fa60_slices_4-5-6-7.zip
mB20809fa60 mutant 7 62B 20809fa60_slices_4-5-6-7.zip
mB5e5a8dc5b mutant 3 38B 5e5a8dc5b_slices_1-2-3-x.zip
mA85f568a1d mutant 1 12A 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 2 54A 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 3 12B 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 4 46A 85f568a1d_slice_4-5-x-6.zip
wBc27f87c73 WT 5 52A c27f87c73_slices_4-5-6-7.zip
wBc27f87c73 WT 6 55B c27f87c73_slices_4-5-6-7.zip
wBc27f87c73 WT 7 52B c27f87c73_slices_4-5-6-7.zip
wAcf610a6f4 WT 1 15A cf610a6f4_slices_1-2-x-x.zip
wAcf610a6f4 WT 2 42A cf610a6f4_slices_1-2-x-x.zip
wAcf610a6f4 WT 3 45A cf610a6f4_slices_3-4-5-6.zip
wA20809fa60 WT 1 58A 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 2 62A 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 3 46B 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 4 12A 20809fa60_slices_4-5-6-7.zip
wA5e5a8dc5b WT 1 46A 5e5a8dc5b_slices_1-2-3-x.zip
wA5e5a8dc5b WT 2 42A 5e5a8dc5b_slices_1-2-3-x.zip
wA5a57423e6 WT 1 46A 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 2 52A 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 3 58B 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 4 15A 5a57423e6_slices_4-5-6-7.zip
wB85f568a1d WT 5 52A 85f568a1d_slice_4-5-x-6.zip
wB85f568a1d WT 6 52B 85f568a1d_slice_4-5-x-6.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAc27f87c73 mutant 1 55A c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 2 52A c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 3 12B c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 4 46A c27f87c73_slices_4-5-6-7.zip
mB342791472 mutant 5 43A 342791472_slices_4-5-6-7.zip
mB342791472 mutant 6 46B 342791472_slices_4-5-6-7.zip
mB342791472 mutant 7 52B 342791472_slices_4-5-6-7.zip
mBcf610a6f4 mutant 4 54A cf610a6f4_slices_3-4-5-6.zip
mBcf610a6f4 mutant 5 46B cf610a6f4_slices_3-4-5-6.zip
mB20809fa60 mutant 6 55B 20809fa60_slices_4-5-6-7.zip
mB20809fa60 mutant 7 62B 20809fa60_slices_4-5-6-7.zip
mB5e5a8dc5b mutant 3 38B 5e5a8dc5b_slices_1-2-3-x.zip
mA85f568a1d mutant 1 12A 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 2 54A 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 3 12B 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 4 46A 85f568a1d_slice_4-5-x-6.zip
wBc27f87c73 WT 5 52A c27f87c73_slices_4-5-6-7.zip
wBc27f87c73 WT 6 55B c27f87c73_slices_4-5-6-7.zip
wBc27f87c73 WT 7 52B c27f87c73_slices_4-5-6-7.zip
wAcf610a6f4 WT 1 15A cf610a6f4_slices_1-2-x-x.zip
wAcf610a6f4 WT 2 42A cf610a6f4_slices_1-2-x-x.zip
wAcf610a6f4 WT 3 45A cf610a6f4_slices_3-4-5-6.zip
wA20809fa60 WT 1 58A 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 2 62A 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 3 46B 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 4 12A 20809fa60_slices_4-5-6-7.zip
wA5e5a8dc5b WT 1 46A 5e5a8dc5b_slices_1-2-3-x.zip
wA5e5a8dc5b WT 2 42A 5e5a8dc5b_slices_1-2-3-x.zip
wA5a57423e6 WT 1 46A 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 2 52A 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 3 58B 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 4 15A 5a57423e6_slices_4-5-6-7.zip
wB85f568a1d WT 5 52A 85f568a1d_slice_4-5-x-6.zip
wB85f568a1d WT 6 52B 85f568a1d_slice_4-5-x-6.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAc27f87c73 mutant 1 55A c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 2 52A c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 3 12B c27f87c73_slices_1-2-3-x.zip
mAc27f87c73 mutant 4 46A c27f87c73_slices_4-5-6-7.zip
mB342791472 mutant 5 43A 342791472_slices_4-5-6-7.zip
mB342791472 mutant 6 46B 342791472_slices_4-5-6-7.zip
mB342791472 mutant 7 52B 342791472_slices_4-5-6-7.zip
mBcf610a6f4 mutant 4 54A cf610a6f4_slices_3-4-5-6.zip
mBcf610a6f4 mutant 5 46B cf610a6f4_slices_3-4-5-6.zip
mB20809fa60 mutant 6 55B 20809fa60_slices_4-5-6-7.zip
mB20809fa60 mutant 7 62B 20809fa60_slices_4-5-6-7.zip
mB5e5a8dc5b mutant 3 38B 5e5a8dc5b_slices_1-2-3-x.zip
mA85f568a1d mutant 1 12A 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 2 54A 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 3 12B 85f568a1d_slice_1-2-3-x.zip
mA85f568a1d mutant 4 46A 85f568a1d_slice_4-5-x-6.zip
wBc27f87c73 WT 5 52A c27f87c73_slices_4-5-6-7.zip
wBc27f87c73 WT 6 55B c27f87c73_slices_4-5-6-7.zip
wBc27f87c73 WT 7 52B c27f87c73_slices_4-5-6-7.zip
wAcf610a6f4 WT 1 15A cf610a6f4_slices_1-2-x-x.zip
wAcf610a6f4 WT 2 42A cf610a6f4_slices_1-2-x-x.zip
wAcf610a6f4 WT 3 45A cf610a6f4_slices_3-4-5-6.zip
wA20809fa60 WT 1 58A 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 2 62A 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 3 46B 20809fa60_slices_1-2-3-x.zip
wA20809fa60 WT 4 12A 20809fa60_slices_4-5-6-7.zip
wA5e5a8dc5b WT 1 46A 5e5a8dc5b_slices_1-2-3-x.zip
wA5e5a8dc5b WT 2 42A 5e5a8dc5b_slices_1-2-3-x.zip
wA5a57423e6 WT 1 46A 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 2 52A 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 3 58B 5a57423e6_slices_1-2-3-x.zip
wA5a57423e6 WT 4 15A 5a57423e6_slices_4-5-6-7.zip
wB85f568a1d WT 5 52A 85f568a1d_slice_4-5-x-6.zip
wB85f568a1d WT 6 52B 85f568a1d_slice_4-5-x-6.zip

Data Files

File NameSizeChecksum (SHA256)
c27f87c73_slices_1-2-3-x.zip32114413df48ebe73368ddaf2943c9fe4cf8e3bc9d79e14e27bc06167867d91db8967c13
c27f87c73_slices_4-5-6-7.zip469595765124ef4ccc330031700e1fd436a3cbbc2bf50b46620d52d3c4e12f6dd585063a
5e5a8dc5b_slices_1-2-3-x.zip309417415266b2e877672e5d7b845af3901895b295d2ef98ea4a5ee701866e39b5e8b1a7
cf610a6f4_slices_3-4-5-6.zip515693196358e3b837b32118a70aae3eecb07e216d32f432d743c9cd978cb21939d82bfa
5a57423e6_slices_1-2-3-x.zip338465403e8887d6aa2fb7615b5f86fabc135bf683dad95275c6866018250e7e5b7f4adc
cf610a6f4_slices_1-2-x-x.zip42138622632ec00ee1b499787d1f2e54970d6eda397864d42ba6a62b559f6f0e07b9d7f1
85f568a1d_slice_4-5-x-6.zip429609098d43dfaa62f0e853b6bde2e514f14371372c1fd97000025bced2983dc331e070
20809fa60_slices_1-2-3-x.zip31204500adbe83fe6b1a906f04c31b98100b98dac073b9ae8de626efc2fd4c96d23f9d27
342791472_slices_4-5-6-7.zip44500013252b2f9f6ba9df432eb0642044e4c65fb670c33e90b563d006bec78771003405
20809fa60_slices_4-5-6-7.zip563949531a87d0f53d5769960b4576725e3127f508c0a322b456f1c459520a408e593c06
85f568a1d_slice_1-2-3-x.zip31560747c8540857b74c2e471b82b00b48b8728f8ef2821e2fc8ece846bf4c95c2c2f2c8
5a57423e6_slices_4-5-6-7.zip42803591b2563770b90321af18a62f3823d031518c571ee882cd1925cca53e2e1af9fb90
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