G2C::Electrophysiology

ZO-1 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices. With heterozygous genotype and no electrophysiological variables significantly affected in mutant slices, this mutation was deemed haplosufficient. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the ZO-1 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 25 (7) 3222 (132) 23 (7) 3184 (98) 0.24 0.84
PPF at 50ms, ampl ratio % 25 (7) 181.4 (3.1) 23 (7) 189.9 (3.5) 0.37 0.11
Burst 1, PPF at 10ms, ampl ratio % 25 (7) 194.1 (5.1) 20 (7) 207.9 (6.3) 0.87 0.051
Burst 1, EPSP3 depr, ampl ratio % 25 (7) 64 (2.4) 20 (7) 62.1 (2.5) 0.14 0.65
Burst 1, ave amplitude µV 25 (7) 1362 (44) 20 (7) 1372 (46) 0.42 0.88
Burst 2, ave amplitude µV 25 (7) 2261 (82) 20 (7) 2283 (86) 0.54 0.85
Burst 3, ave amplitude µV 25 (7) 2508 (94) 20 (7) 2543 (91) 0.49 0.8
Burst 4, ave amplitude µV 25 (7) 2561 (92) 20 (7) 2606 (85) 0.63 0.71
Burst 5, ave amplitude µV 25 (7) 2520 (85) 20 (7) 2561 (76) 0.59 0.72
Burst 6, ave amplitude µV 25 (7) 2453 (82) 20 (7) 2488 (71) 0.7 0.73
Burst 7, ave amplitude µV 25 (7) 2373 (80) 20 (7) 2401 (70) 0.71 0.77
Burst 8, ave amplitude µV 25 (7) 2311 (78) 20 (7) 2342 (65) 0.7 0.74
Burst 9, ave amplitude µV 25 (7) 2249 (80) 20 (7) 2290 (65) 0.7 0.68
Burst 10, ave amplitude µV 25 (7) 2195 (81) 20 (7) 2234 (64) 0.7 0.69
Burst 2-4 ave amplitude vs Burst 1 % 25 (7) 179.8 (3.5) 20 (7) 181.3 (3.4) 0.46 0.77
Burst 8-10 ave amplitude vs Burst 2-4 % 25 (7) 92.4 (0.9) 20 (7) 93 (1.4) 0.11 0.78
LTP vs PTP, amplitude ratio % 25 (7) 79.5 (0.9) 20 (7) 76.4 (2.4) 1.3e-06 *** 0.45
LTP based on amplitude % 25 (7) 174.5 (4) 20 (7) 174.1 (4.4) 0.36 0.95

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 25 (7) 3222 (132) 23 (7) 3184 (98) 0.24 0.84

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 25 (7) 181.4 (3.1) 23 (7) 189.9 (3.5) 0.37 0.11

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 25 (7) 194.1 (5.1) 20 (7) 207.9 (6.3) 0.87 0.051
Burst 1, EPSP3 depr, ampl ratio % 25 (7) 64 (2.4) 20 (7) 62.1 (2.5) 0.14 0.65
Burst 1, ave amplitude µV 25 (7) 1362 (44) 20 (7) 1372 (46) 0.42 0.88
Burst 2, ave amplitude µV 25 (7) 2261 (82) 20 (7) 2283 (86) 0.54 0.85
Burst 3, ave amplitude µV 25 (7) 2508 (94) 20 (7) 2543 (91) 0.49 0.8
Burst 4, ave amplitude µV 25 (7) 2561 (92) 20 (7) 2606 (85) 0.63 0.71
Burst 5, ave amplitude µV 25 (7) 2520 (85) 20 (7) 2561 (76) 0.59 0.72
Burst 6, ave amplitude µV 25 (7) 2453 (82) 20 (7) 2488 (71) 0.7 0.73
Burst 7, ave amplitude µV 25 (7) 2373 (80) 20 (7) 2401 (70) 0.71 0.77
Burst 8, ave amplitude µV 25 (7) 2311 (78) 20 (7) 2342 (65) 0.7 0.74
Burst 9, ave amplitude µV 25 (7) 2249 (80) 20 (7) 2290 (65) 0.7 0.68
Burst 10, ave amplitude µV 25 (7) 2195 (81) 20 (7) 2234 (64) 0.7 0.69
Burst 2-4 ave amplitude vs Burst 1 % 25 (7) 179.8 (3.5) 20 (7) 181.3 (3.4) 0.46 0.77
Burst 8-10 ave amplitude vs Burst 2-4 % 25 (7) 92.4 (0.9) 20 (7) 93 (1.4) 0.11 0.78

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 25 (7) 79.5 (0.9) 20 (7) 76.4 (2.4) 1.3e-06 *** 0.45
LTP based on amplitude % 25 (7) 174.5 (4) 20 (7) 174.1 (4.4) 0.36 0.95

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT7
mutant7

 

Individual Mice

Mouse IDGenotype
wB2186d32f5WT
wB4a2120695WT
wA56f613f62WT
wAf24382fd0WT
wBd4af8594cWT
wA5b67d6aebWT
wB75955765cWT
mAd4af8594cmutant
mA2186d32f5mutant
mB75955765cmutant
mB56f613f62mutant
mBf24382fd0mutant
mB5b67d6aebmutant
mA4a2120695mutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBf24382fd0 mutant 5 42A f24382fd0_slices_4-5-6-7.zip
mBf24382fd0 mutant 6 46B f24382fd0_slices_4-5-6-7.zip
mBf24382fd0 mutant 7 61B f24382fd0_slices_4-5-6-7.zip
mA2186d32f5 mutant 1 46A 2186d32f5_slices_1-2-3-x.zip
mA2186d32f5 mutant 2 52A 2186d32f5_slices_1-2-3-x.zip
mA2186d32f5 mutant 3 46B 2186d32f5_slices_1-2-3-x.zip
mA4a2120695 mutant 1 12A 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 2 43A 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 3 12B 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 6 12B 4a2120695_slices_4-5-6-7.zip
mB56f613f62 mutant 2 43A 56f613f62_slices_1-2-3-x.zip
mB56f613f62 mutant 5 52A 56f613f62_slices_4-5-6-7.zip
mB56f613f62 mutant 6 67B 56f613f62_slices_4-5-6-7.zip
mB56f613f62 mutant 7 54B 56f613f62_slices_4-5-6-7.zip
mB5b67d6aeb mutant 5 54A 5b67d6aeb_slices_4-5-6-7.zip
mB5b67d6aeb mutant 6 58B 5b67d6aeb_slices_4-5-6-7.zip
mB5b67d6aeb mutant 7 62B 5b67d6aeb_slices_4-5-6-7.zip
mAd4af8594c mutant 1 12A d4af8594c_slices_1-2-3-x.zip
mAd4af8594c mutant 2 62A d4af8594c_slices_1-2-3-x.zip
mAd4af8594c mutant 3 15B d4af8594c_slices_1-2-3-x.zip
mB75955765c mutant 1 12A 75955765c_slices_1-2-3-x.zip
mB75955765c mutant 2 52A 75955765c_slices_1-2-3-x.zip
mB75955765c mutant 3 46B 75955765c_slices_1-2-3-x.zip
wAf24382fd0 WT 1 55A f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 2 52A f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 3 12B f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 4 24A f24382fd0_slices_4-5-6-7.zip
wB2186d32f5 WT 5 42A 2186d32f5_slices_4-5-6-7.zip
wB2186d32f5 WT 6 55B 2186d32f5_slices_4-5-6-7.zip
wB2186d32f5 WT 7 52B 2186d32f5_slices_4-5-6-7.zip
wB4a2120695 WT 4 12A 4a2120695_slices_4-5-6-7.zip
wB4a2120695 WT 5 62A 4a2120695_slices_4-5-6-7.zip
wB4a2120695 WT 7 52B 4a2120695_slices_4-5-6-7.zip
wA56f613f62 WT 1 55A 56f613f62_slices_1-2-3-x.zip
wA56f613f62 WT 3 12B 56f613f62_slices_1-2-3-x.zip
wA56f613f62 WT 4 12A 56f613f62_slices_4-5-6-7.zip
wA5b67d6aeb WT 1 46A 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 2 62A 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 3 46B 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 4 38A 5b67d6aeb_slices_4-5-6-7.zip
wBd4af8594c WT 4 12A d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 5 62A d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 6 38B d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 7 62B d4af8594c_slices_4-5-6-7.zip
wB75955765c WT 4 12A 75955765c_slices_4-5-6-7.zip
wB75955765c WT 5 52A 75955765c_slices_4-5-6-7.zip
wB75955765c WT 6 58B 75955765c_slices_4-5-6-7.zip
wB75955765c WT 7 71B 75955765c_slices_4-5-6-7.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBf24382fd0 mutant 5 42A f24382fd0_slices_4-5-6-7.zip
mBf24382fd0 mutant 6 46B f24382fd0_slices_4-5-6-7.zip
mBf24382fd0 mutant 7 61B f24382fd0_slices_4-5-6-7.zip
mA2186d32f5 mutant 1 46A 2186d32f5_slices_1-2-3-x.zip
mA2186d32f5 mutant 2 52A 2186d32f5_slices_1-2-3-x.zip
mA2186d32f5 mutant 3 46B 2186d32f5_slices_1-2-3-x.zip
mA4a2120695 mutant 1 12A 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 2 43A 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 3 12B 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 6 12B 4a2120695_slices_4-5-6-7.zip
mB56f613f62 mutant 2 43A 56f613f62_slices_1-2-3-x.zip
mB56f613f62 mutant 5 52A 56f613f62_slices_4-5-6-7.zip
mB56f613f62 mutant 6 67B 56f613f62_slices_4-5-6-7.zip
mB56f613f62 mutant 7 54B 56f613f62_slices_4-5-6-7.zip
mB5b67d6aeb mutant 5 54A 5b67d6aeb_slices_4-5-6-7.zip
mB5b67d6aeb mutant 6 58B 5b67d6aeb_slices_4-5-6-7.zip
mB5b67d6aeb mutant 7 62B 5b67d6aeb_slices_4-5-6-7.zip
mAd4af8594c mutant 1 12A d4af8594c_slices_1-2-3-x.zip
mAd4af8594c mutant 2 62A d4af8594c_slices_1-2-3-x.zip
mAd4af8594c mutant 3 15B d4af8594c_slices_1-2-3-x.zip
mB75955765c mutant 1 12A 75955765c_slices_1-2-3-x.zip
mB75955765c mutant 2 52A 75955765c_slices_1-2-3-x.zip
mB75955765c mutant 3 46B 75955765c_slices_1-2-3-x.zip
wAf24382fd0 WT 1 55A f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 2 52A f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 3 12B f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 4 24A f24382fd0_slices_4-5-6-7.zip
wB2186d32f5 WT 5 42A 2186d32f5_slices_4-5-6-7.zip
wB2186d32f5 WT 6 55B 2186d32f5_slices_4-5-6-7.zip
wB2186d32f5 WT 7 52B 2186d32f5_slices_4-5-6-7.zip
wB4a2120695 WT 4 12A 4a2120695_slices_4-5-6-7.zip
wB4a2120695 WT 5 62A 4a2120695_slices_4-5-6-7.zip
wB4a2120695 WT 7 52B 4a2120695_slices_4-5-6-7.zip
wA56f613f62 WT 1 55A 56f613f62_slices_1-2-3-x.zip
wA56f613f62 WT 3 12B 56f613f62_slices_1-2-3-x.zip
wA56f613f62 WT 4 12A 56f613f62_slices_4-5-6-7.zip
wA5b67d6aeb WT 1 46A 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 2 62A 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 3 46B 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 4 38A 5b67d6aeb_slices_4-5-6-7.zip
wBd4af8594c WT 4 12A d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 5 62A d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 6 38B d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 7 62B d4af8594c_slices_4-5-6-7.zip
wB75955765c WT 4 12A 75955765c_slices_4-5-6-7.zip
wB75955765c WT 5 52A 75955765c_slices_4-5-6-7.zip
wB75955765c WT 6 58B 75955765c_slices_4-5-6-7.zip
wB75955765c WT 7 71B 75955765c_slices_4-5-6-7.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBf24382fd0 mutant 5 42A f24382fd0_slices_4-5-6-7.zip
mBf24382fd0 mutant 6 46B f24382fd0_slices_4-5-6-7.zip
mA2186d32f5 mutant 1 46A 2186d32f5_slices_1-2-3-x.zip
mA2186d32f5 mutant 2 52A 2186d32f5_slices_1-2-3-x.zip
mA2186d32f5 mutant 3 46B 2186d32f5_slices_1-2-3-x.zip
mA4a2120695 mutant 1 12A 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 2 43A 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 3 12B 4a2120695_slices_1-2-3-x.zip
mA4a2120695 mutant 6 12B 4a2120695_slices_4-5-6-7.zip
mB56f613f62 mutant 5 52A 56f613f62_slices_4-5-6-7.zip
mB56f613f62 mutant 6 67B 56f613f62_slices_4-5-6-7.zip
mB56f613f62 mutant 7 54B 56f613f62_slices_4-5-6-7.zip
mB5b67d6aeb mutant 5 54A 5b67d6aeb_slices_4-5-6-7.zip
mB5b67d6aeb mutant 6 58B 5b67d6aeb_slices_4-5-6-7.zip
mB5b67d6aeb mutant 7 62B 5b67d6aeb_slices_4-5-6-7.zip
mAd4af8594c mutant 1 12A d4af8594c_slices_1-2-3-x.zip
mAd4af8594c mutant 2 62A d4af8594c_slices_1-2-3-x.zip
mB75955765c mutant 1 12A 75955765c_slices_1-2-3-x.zip
mB75955765c mutant 2 52A 75955765c_slices_1-2-3-x.zip
mB75955765c mutant 3 46B 75955765c_slices_1-2-3-x.zip
wAf24382fd0 WT 1 55A f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 2 52A f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 3 12B f24382fd0_slices_1-2-3-x.zip
wAf24382fd0 WT 4 24A f24382fd0_slices_4-5-6-7.zip
wB2186d32f5 WT 5 42A 2186d32f5_slices_4-5-6-7.zip
wB2186d32f5 WT 6 55B 2186d32f5_slices_4-5-6-7.zip
wB2186d32f5 WT 7 52B 2186d32f5_slices_4-5-6-7.zip
wB4a2120695 WT 4 12A 4a2120695_slices_4-5-6-7.zip
wB4a2120695 WT 5 62A 4a2120695_slices_4-5-6-7.zip
wB4a2120695 WT 7 52B 4a2120695_slices_4-5-6-7.zip
wA56f613f62 WT 1 55A 56f613f62_slices_1-2-3-x.zip
wA56f613f62 WT 3 12B 56f613f62_slices_1-2-3-x.zip
wA56f613f62 WT 4 12A 56f613f62_slices_4-5-6-7.zip
wA5b67d6aeb WT 1 46A 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 2 62A 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 3 46B 5b67d6aeb_slices_1-2-3-x.zip
wA5b67d6aeb WT 4 38A 5b67d6aeb_slices_4-5-6-7.zip
wBd4af8594c WT 4 12A d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 5 62A d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 6 38B d4af8594c_slices_4-5-6-7.zip
wBd4af8594c WT 7 62B d4af8594c_slices_4-5-6-7.zip
wB75955765c WT 4 12A 75955765c_slices_4-5-6-7.zip
wB75955765c WT 5 52A 75955765c_slices_4-5-6-7.zip
wB75955765c WT 6 58B 75955765c_slices_4-5-6-7.zip
wB75955765c WT 7 71B 75955765c_slices_4-5-6-7.zip

Data Files

File NameSizeChecksum (SHA256)
d4af8594c_slices_4-5-6-7.zip5143162362173fe1a23d7f2887e56d5901bb20830ac97c31ff16b5eb7cb2c8d29c0a962d
56f613f62_slices_1-2-3-x.zip34410893c4e40fca8c89ccd932d190e06885c614b9b276b10c9dc63731ed906a84f8fa76
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