G2C::Electrophysiology

GKAP2 knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed little overall electrophysiological difference from wildtype slices, with no electrophysiological variables significantly affected by this mutation. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the GKAP2 knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 23 (7) 2870 (140) 24 (8) 2850 (130) 0.011 * 0.94
PPF at 50ms, ampl ratio % 23 (7) 188.1 (3.2) 24 (8) 190.1 (5) 0.08 0.78
Burst 1, PPF at 10ms, ampl ratio % 23 (7) 208.1 (8.9) 24 (8) 197.6 (7.4) 0.093 0.47
Burst 1, EPSP3 depr, ampl ratio % 23 (7) 65.2 (1.9) 24 (8) 62.8 (2) 0.29 0.45
Burst 1, ave amplitude µV 23 (7) 1231 (58) 24 (8) 1211 (50) 0.0017 ** 0.86
Burst 2, ave amplitude µV 23 (7) 2085 (95) 24 (8) 1970 (94) 0.0024 ** 0.56
Burst 3, ave amplitude µV 23 (7) 2339 (93) 24 (8) 2231 (93) 0.011 * 0.56
Burst 4, ave amplitude µV 23 (7) 2401 (84) 24 (8) 2312 (86) 0.016 * 0.6
Burst 5, ave amplitude µV 23 (7) 2350 (77) 24 (8) 2267 (76) 0.015 * 0.58
Burst 6, ave amplitude µV 23 (7) 2295 (72) 24 (8) 2223 (71) 0.031 * 0.59
Burst 7, ave amplitude µV 23 (7) 2225 (70) 24 (8) 2178 (72) 0.019 * 0.73
Burst 8, ave amplitude µV 23 (7) 2163 (67) 24 (8) 2142 (69) 0.021 * 0.88
Burst 9, ave amplitude µV 23 (7) 2102 (66) 24 (8) 2107 (69) 0.029 * 0.97
Burst 10, ave amplitude µV 23 (7) 2050 (68) 24 (8) 2103 (71) 0.02 * 0.7
Burst 2-4 ave amplitude vs Burst 1 % 23 (7) 187.8 (4.6) 24 (8) 180.3 (4.2) 0.09 0.35
Burst 8-10 ave amplitude vs Burst 2-4 % 23 (7) 94.1 (2.1) 24 (8) 99.6 (1.7) 0.17 0.11
LTP vs PTP, amplitude ratio % 23 (7) 76.6 (1.3) 24 (8) 74.1 (1.2) 0.15 0.25
LTP based on amplitude % 23 (7) 175.7 (7) 24 (8) 173.7 (4.3) 0.16 0.84

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 23 (7) 2870 (140) 24 (8) 2850 (130) 0.011 * 0.94

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 23 (7) 188.1 (3.2) 24 (8) 190.1 (5) 0.08 0.78

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 23 (7) 208.1 (8.9) 24 (8) 197.6 (7.4) 0.093 0.47
Burst 1, EPSP3 depr, ampl ratio % 23 (7) 65.2 (1.9) 24 (8) 62.8 (2) 0.29 0.45
Burst 1, ave amplitude µV 23 (7) 1231 (58) 24 (8) 1211 (50) 0.0017 ** 0.86
Burst 2, ave amplitude µV 23 (7) 2085 (95) 24 (8) 1970 (94) 0.0024 ** 0.56
Burst 3, ave amplitude µV 23 (7) 2339 (93) 24 (8) 2231 (93) 0.011 * 0.56
Burst 4, ave amplitude µV 23 (7) 2401 (84) 24 (8) 2312 (86) 0.016 * 0.6
Burst 5, ave amplitude µV 23 (7) 2350 (77) 24 (8) 2267 (76) 0.015 * 0.58
Burst 6, ave amplitude µV 23 (7) 2295 (72) 24 (8) 2223 (71) 0.031 * 0.59
Burst 7, ave amplitude µV 23 (7) 2225 (70) 24 (8) 2178 (72) 0.019 * 0.73
Burst 8, ave amplitude µV 23 (7) 2163 (67) 24 (8) 2142 (69) 0.021 * 0.88
Burst 9, ave amplitude µV 23 (7) 2102 (66) 24 (8) 2107 (69) 0.029 * 0.97
Burst 10, ave amplitude µV 23 (7) 2050 (68) 24 (8) 2103 (71) 0.02 * 0.7
Burst 2-4 ave amplitude vs Burst 1 % 23 (7) 187.8 (4.6) 24 (8) 180.3 (4.2) 0.09 0.35
Burst 8-10 ave amplitude vs Burst 2-4 % 23 (7) 94.1 (2.1) 24 (8) 99.6 (1.7) 0.17 0.11

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 23 (7) 76.6 (1.3) 24 (8) 74.1 (1.2) 0.15 0.25
LTP based on amplitude % 23 (7) 175.7 (7) 24 (8) 173.7 (4.3) 0.16 0.84

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT7
mutant8

 

Individual Mice

Mouse IDGenotype
wAb4dc1fee0WT
wBe7581ce2bWT
wBfed1ea89dWT
wAfed1ea89dWT
wAabee3db08WT
wAe6dc38448WT
wBe558ec868WT
mB701edb755mutant
mA701edb755mutant
mAbbaa2ce26mutant
mBb4dc1fee0mutant
mBabee3db08mutant
mBe6dc38448mutant
mAe558ec868mutant
mAe7581ce2bmutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAbbaa2ce26 mutant 1 46A bbaa2ce26_slices_1-2-3-x.zip
mAbbaa2ce26 mutant 2 73A bbaa2ce26_slices_1-2-3-x.zip
mBe6dc38448 mutant 4 12A e6dc38448_slices_4-x-5-6.zip
mBe6dc38448 mutant 5 58B e6dc38448_slices_4-x-5-6.zip
mBe6dc38448 mutant 6 43B e6dc38448_slices_4-x-5-6.zip
mAe7581ce2b mutant 1 46A e7581ce2b_slices_1-x-2-x.zip
mAe7581ce2b mutant 2 38B e7581ce2b_slices_1-x-2-x.zip
mBb4dc1fee0 mutant 3 58A b4dc1fee0_slices_3-4-5-x.zip
mBb4dc1fee0 mutant 4 41A b4dc1fee0_slices_3-4-5-x.zip
mBb4dc1fee0 mutant 5 58B b4dc1fee0_slices_3-4-5-x.zip
mBabee3db08 mutant 4 55A abee3db08_slices_4-5-6-7.zip
mBabee3db08 mutant 5 54A abee3db08_slices_4-5-6-7.zip
mBabee3db08 mutant 7 73B abee3db08_slices_4-5-6-7.zip
mA701edb755 mutant 1 15A 701edb755_slices_1-2-3-x.zip
mA701edb755 mutant 2 52A 701edb755_slices_1-2-3-x.zip
mB701edb755 mutant 3 46B 701edb755_slices_1-2-3-x.zip
mA701edb755 mutant 4 55A 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 5 62A 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 6 46B 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 7 54B 701edb755_slices_4-5-6-7.zip
mAe558ec868 mutant 1 46A e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 2 54A e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 3 46B e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 4 67A e558ec868_slices_4-5-6-7.zip
wAe6dc38448 WT 1 58A e6dc38448_slices_1-2-3-x.zip
wAe6dc38448 WT 2 41A e6dc38448_slices_1-2-3-x.zip
wAe6dc38448 WT 3 46B e6dc38448_slices_1-2-3-x.zip
wBe7581ce2b WT 3 58A e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 4 61A e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 5 58B e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 6 62B e7581ce2b_slices_3-4-5-6.zip
wAb4dc1fee0 WT 1 46A b4dc1fee0_slice_1-x-2-x.zip
wAb4dc1fee0 WT 2 12B b4dc1fee0_slice_1-x-2-x.zip
wAabee3db08 WT 1 46A abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 2 62A abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 3 46B abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 6 46B abee3db08_slices_4-5-6-7.zip
wBe558ec868 WT 5 52A e558ec868_slices_4-5-6-7.zip
wBe558ec868 WT 6 58B e558ec868_slices_4-5-6-7.zip
wBe558ec868 WT 7 61B e558ec868_slices_4-5-6-7.zip
wAfed1ea89d WT 1 46A fed1ea89d_slices_1-2-3-x.zip
wAfed1ea89d WT 2 52A fed1ea89d_slices_1-2-3-x.zip
wBfed1ea89d WT 3 46B fed1ea89d_slices_1-2-3-x.zip
wAfed1ea89d WT 4 55A fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 5 42A fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 6 46B fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 7 43B fed1ea89d_slices_4-5-6-7.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAbbaa2ce26 mutant 1 46A bbaa2ce26_slices_1-2-3-x.zip
mAbbaa2ce26 mutant 2 73A bbaa2ce26_slices_1-2-3-x.zip
mBe6dc38448 mutant 4 12A e6dc38448_slices_4-x-5-6.zip
mBe6dc38448 mutant 5 58B e6dc38448_slices_4-x-5-6.zip
mBe6dc38448 mutant 6 43B e6dc38448_slices_4-x-5-6.zip
mAe7581ce2b mutant 1 46A e7581ce2b_slices_1-x-2-x.zip
mAe7581ce2b mutant 2 38B e7581ce2b_slices_1-x-2-x.zip
mBb4dc1fee0 mutant 3 58A b4dc1fee0_slices_3-4-5-x.zip
mBb4dc1fee0 mutant 4 41A b4dc1fee0_slices_3-4-5-x.zip
mBb4dc1fee0 mutant 5 58B b4dc1fee0_slices_3-4-5-x.zip
mBabee3db08 mutant 4 55A abee3db08_slices_4-5-6-7.zip
mBabee3db08 mutant 5 54A abee3db08_slices_4-5-6-7.zip
mBabee3db08 mutant 7 73B abee3db08_slices_4-5-6-7.zip
mA701edb755 mutant 1 15A 701edb755_slices_1-2-3-x.zip
mA701edb755 mutant 2 52A 701edb755_slices_1-2-3-x.zip
mB701edb755 mutant 3 46B 701edb755_slices_1-2-3-x.zip
mA701edb755 mutant 4 55A 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 5 62A 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 6 46B 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 7 54B 701edb755_slices_4-5-6-7.zip
mAe558ec868 mutant 1 46A e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 2 54A e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 3 46B e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 4 67A e558ec868_slices_4-5-6-7.zip
wAe6dc38448 WT 1 58A e6dc38448_slices_1-2-3-x.zip
wAe6dc38448 WT 2 41A e6dc38448_slices_1-2-3-x.zip
wAe6dc38448 WT 3 46B e6dc38448_slices_1-2-3-x.zip
wBe7581ce2b WT 3 58A e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 4 61A e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 5 58B e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 6 62B e7581ce2b_slices_3-4-5-6.zip
wAb4dc1fee0 WT 1 46A b4dc1fee0_slice_1-x-2-x.zip
wAb4dc1fee0 WT 2 12B b4dc1fee0_slice_1-x-2-x.zip
wAabee3db08 WT 1 46A abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 2 62A abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 3 46B abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 6 46B abee3db08_slices_4-5-6-7.zip
wBe558ec868 WT 5 52A e558ec868_slices_4-5-6-7.zip
wBe558ec868 WT 6 58B e558ec868_slices_4-5-6-7.zip
wBe558ec868 WT 7 61B e558ec868_slices_4-5-6-7.zip
wAfed1ea89d WT 1 46A fed1ea89d_slices_1-2-3-x.zip
wAfed1ea89d WT 2 52A fed1ea89d_slices_1-2-3-x.zip
wBfed1ea89d WT 3 46B fed1ea89d_slices_1-2-3-x.zip
wAfed1ea89d WT 4 55A fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 5 42A fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 6 46B fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 7 43B fed1ea89d_slices_4-5-6-7.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mAbbaa2ce26 mutant 1 46A bbaa2ce26_slices_1-2-3-x.zip
mAbbaa2ce26 mutant 2 73A bbaa2ce26_slices_1-2-3-x.zip
mBe6dc38448 mutant 4 12A e6dc38448_slices_4-x-5-6.zip
mBe6dc38448 mutant 5 58B e6dc38448_slices_4-x-5-6.zip
mBe6dc38448 mutant 6 43B e6dc38448_slices_4-x-5-6.zip
mAe7581ce2b mutant 1 46A e7581ce2b_slices_1-x-2-x.zip
mAe7581ce2b mutant 2 38B e7581ce2b_slices_1-x-2-x.zip
mBb4dc1fee0 mutant 3 58A b4dc1fee0_slices_3-4-5-x.zip
mBb4dc1fee0 mutant 4 41A b4dc1fee0_slices_3-4-5-x.zip
mBb4dc1fee0 mutant 5 58B b4dc1fee0_slices_3-4-5-x.zip
mBabee3db08 mutant 4 55A abee3db08_slices_4-5-6-7.zip
mBabee3db08 mutant 5 54A abee3db08_slices_4-5-6-7.zip
mBabee3db08 mutant 7 73B abee3db08_slices_4-5-6-7.zip
mA701edb755 mutant 1 15A 701edb755_slices_1-2-3-x.zip
mA701edb755 mutant 2 52A 701edb755_slices_1-2-3-x.zip
mB701edb755 mutant 3 46B 701edb755_slices_1-2-3-x.zip
mA701edb755 mutant 4 55A 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 5 62A 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 6 46B 701edb755_slices_4-5-6-7.zip
mB701edb755 mutant 7 54B 701edb755_slices_4-5-6-7.zip
mAe558ec868 mutant 1 46A e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 2 54A e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 3 46B e558ec868_slices_1-2-3-x.zip
mAe558ec868 mutant 4 67A e558ec868_slices_4-5-6-7.zip
wAe6dc38448 WT 1 58A e6dc38448_slices_1-2-3-x.zip
wAe6dc38448 WT 2 41A e6dc38448_slices_1-2-3-x.zip
wAe6dc38448 WT 3 46B e6dc38448_slices_1-2-3-x.zip
wBe7581ce2b WT 3 58A e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 4 61A e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 5 58B e7581ce2b_slices_3-4-5-6.zip
wBe7581ce2b WT 6 62B e7581ce2b_slices_3-4-5-6.zip
wAb4dc1fee0 WT 1 46A b4dc1fee0_slice_1-x-2-x.zip
wAb4dc1fee0 WT 2 12B b4dc1fee0_slice_1-x-2-x.zip
wAabee3db08 WT 1 46A abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 2 62A abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 3 46B abee3db08_slices_1-2-3-x.zip
wAabee3db08 WT 6 46B abee3db08_slices_4-5-6-7.zip
wBe558ec868 WT 5 52A e558ec868_slices_4-5-6-7.zip
wBe558ec868 WT 6 58B e558ec868_slices_4-5-6-7.zip
wBe558ec868 WT 7 61B e558ec868_slices_4-5-6-7.zip
wAfed1ea89d WT 1 46A fed1ea89d_slices_1-2-3-x.zip
wAfed1ea89d WT 2 52A fed1ea89d_slices_1-2-3-x.zip
wBfed1ea89d WT 3 46B fed1ea89d_slices_1-2-3-x.zip
wAfed1ea89d WT 4 55A fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 5 42A fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 6 46B fed1ea89d_slices_4-5-6-7.zip
wBfed1ea89d WT 7 43B fed1ea89d_slices_4-5-6-7.zip

Data Files

File NameSizeChecksum (SHA256)
701edb755_slices_1-2-3-x.zip352919001d2d4223f6a38f77e7def083550972a614749e6912625732f65bc51541968d38
fed1ea89d_slices_4-5-6-7.zip7862269359cd11f3e50a2cd3055345754194312e82cd8eff6013573c595a525ae24a14c8
b4dc1fee0_slices_3-4-5-x.zip45445260bade77b334f3dc25dc1620040bd868bffc21b4224fd33cbd89eccba22d8135e8
701edb755_slices_4-5-6-7.zip4516619463cd6fc3551c4991af3d38219c359c93b3c8dc0b27af4cc6ebcdaa8877e01b32
fed1ea89d_slices_1-2-3-x.zip55881733fe288f6817fabcad4b125bbaf99722de43c99473a9035f9490680b1561ec45bd
e6dc38448_slices_1-2-3-x.zip39477975858402dfc587748295f921b782457d5311dde11cd6deefbdf8ca1927a65dc7de
e6dc38448_slices_4-x-5-6.zip52225105a54e30da21a8b4d3215290dfd330cb1f1d75943ce9b7b5531cd541ac2c8f2680
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