G2C::Electrophysiology

GKAP knock-out mouse

S.G.N. Grant and the G2C Consortium

Corresponding email: Seth.Grant@ed.ac.uk  

 

Overview: Effect sizes (Cohen d) for 18 electrophysiology variables

Mutant slices showed very large overall electrophysiological difference from wildtype slices, with four of nine variables significantly impacted in these mutant slices. In the basal synaptic transmission experiment, max fEPSP amplitude was significantly decreased in mutants. In the theta burst stimulation experiment, three electrophysiological variables of five were significantly impacted in mutants. Paired pulse facilitation and long term potentiation experiments were unaffected. Note that in the theta burst analysis, the tenth burst is analysed as representative of individual burst amplitude. Definitions of variables may be found below.

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The G2CMine data warehouse provides slice group summaries and individual mouse observations from the GKAP knock-out line phenotyping.

 

Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude. PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio. Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4. LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 16 (5) 3000 (160) 16 (5) 1680 (110) 0.5 0.00025 ***
PPF at 50ms, ampl ratio % 16 (5) 175.4 (5.4) 16 (5) 190.2 (4.2) 0.082 0.14
Burst 1, PPF at 10ms, ampl ratio % 16 (5) 184.2 (9.3) 16 (5) 205.6 (6.7) 0.042 * 0.21
Burst 1, EPSP3 depr, ampl ratio % 16 (5) 64.2 (3.2) 16 (5) 83.3 (1.7) 0.074 0.0045 **
Burst 1, ave amplitude µV 16 (5) 1323 (57) 16 (5) 863 (52) 0.11 0.0018 **
Burst 2, ave amplitude µV 16 (5) 2123 (75) 16 (5) 1501 (83) 0.32 0.0013 **
Burst 3, ave amplitude µV 16 (5) 2360 (90) 16 (5) 1702 (107) 0.23 0.0042 **
Burst 4, ave amplitude µV 16 (5) 2445 (87) 16 (5) 1849 (115) 0.3 0.0068 **
Burst 5, ave amplitude µV 16 (5) 2406 (81) 16 (5) 1895 (115) 0.39 0.01 *
Burst 6, ave amplitude µV 16 (5) 2336 (75) 16 (5) 1887 (111) 0.42 0.014 *
Burst 7, ave amplitude µV 16 (5) 2273 (77) 16 (5) 1845 (103) 0.48 0.012 *
Burst 8, ave amplitude µV 16 (5) 2177 (72) 16 (5) 1803 (104) 0.52 0.018 *
Burst 9, ave amplitude µV 16 (5) 2124 (75) 16 (5) 1757 (104) 0.5 0.022 *
Burst 10, ave amplitude µV 16 (5) 2079 (76) 16 (5) 1721 (101) 0.49 0.024 *
Burst 2-4 ave amplitude vs Burst 1 % 16 (5) 176.6 (5.8) 16 (5) 192.8 (5.5) 0.39 0.09
Burst 8-10 ave amplitude vs Burst 2-4 % 16 (5) 92.1 (1) 16 (5) 104.5 (1) 0.46 4e-05 ***
LTP vs PTP, amplitude ratio % 16 (5) 79 (1.3) 16 (5) 78.8 (1.5) 0.95 0.84
LTP based on amplitude % 16 (5) 173.7 (7.7) 16 (5) 200.3 (8.8) 0.25 0.083

 

Basal Synaptic Transmission

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Variables shown are: Max fEPSP amplitude, Maximum field excitatory postsynaptic potential (fEPSP) amplitude.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Max fEPSP amplitude µV 16 (5) 3000 (160) 16 (5) 1680 (110) 0.5 0.00025 ***

 

Paired Pulse Facilitation

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Variables shown are: PPF at 50ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 50ms, amplitude ratio.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
PPF at 50ms, ampl ratio % 16 (5) 175.4 (5.4) 16 (5) 190.2 (4.2) 0.082 0.14

 

Theta Burst Stimulation

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Variables shown are: Burst 1, PPF at 10ms, ampl ratio, Paired pulse facilitation (PPF), pulses separated by 10ms, amplitude ratio, observed during first two pulses of the first 100Hz burst during theta-burst stimulation. Burst 1, EPSP3 depr, ampl ratio, Depression observed in third fEPSP relative to the second fEPSP of the first 100Hz burst, amplitude ratio. Burst 1, ave amplitude, Average amplitude of four fEPSPs in first burst. Burst 2, ave amplitude, Average amplitude of four fEPSPs in second burst. Burst 3, ave amplitude, Average amplitude of four fEPSPs in third burst. Burst 4, ave amplitude, Average amplitude of four fEPSPs in fourth burst. Burst 5, ave amplitude, Average amplitude of four fEPSPs in fifth burst. Burst 6, ave amplitude, Average amplitude of four fEPSPs in sixth burst. Burst 7, ave amplitude, Average amplitude of four fEPSPs in seventh burst. Burst 8, ave amplitude, Average amplitude of four fEPSPs in eighth burst. Burst 9, ave amplitude, Average amplitude of four fEPSPs in ninth burst. Burst 10, ave amplitude, Average amplitude of four fEPSPs in tenth burst. Burst 2-4 ave amplitude vs Burst 1, Facilitation observed in average amplitude of bursts 2-4, relative to average amplitude of burst 1. Burst 8-10 ave amplitude vs Burst 2-4, Depression observed in average amplitude of bursts 8-10, relative to average amplitude of bursts 2-4.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
Burst 1, PPF at 10ms, ampl ratio % 16 (5) 184.2 (9.3) 16 (5) 205.6 (6.7) 0.042 * 0.21
Burst 1, EPSP3 depr, ampl ratio % 16 (5) 64.2 (3.2) 16 (5) 83.3 (1.7) 0.074 0.0045 **
Burst 1, ave amplitude µV 16 (5) 1323 (57) 16 (5) 863 (52) 0.11 0.0018 **
Burst 2, ave amplitude µV 16 (5) 2123 (75) 16 (5) 1501 (83) 0.32 0.0013 **
Burst 3, ave amplitude µV 16 (5) 2360 (90) 16 (5) 1702 (107) 0.23 0.0042 **
Burst 4, ave amplitude µV 16 (5) 2445 (87) 16 (5) 1849 (115) 0.3 0.0068 **
Burst 5, ave amplitude µV 16 (5) 2406 (81) 16 (5) 1895 (115) 0.39 0.01 *
Burst 6, ave amplitude µV 16 (5) 2336 (75) 16 (5) 1887 (111) 0.42 0.014 *
Burst 7, ave amplitude µV 16 (5) 2273 (77) 16 (5) 1845 (103) 0.48 0.012 *
Burst 8, ave amplitude µV 16 (5) 2177 (72) 16 (5) 1803 (104) 0.52 0.018 *
Burst 9, ave amplitude µV 16 (5) 2124 (75) 16 (5) 1757 (104) 0.5 0.022 *
Burst 10, ave amplitude µV 16 (5) 2079 (76) 16 (5) 1721 (101) 0.49 0.024 *
Burst 2-4 ave amplitude vs Burst 1 % 16 (5) 176.6 (5.8) 16 (5) 192.8 (5.5) 0.39 0.09
Burst 8-10 ave amplitude vs Burst 2-4 % 16 (5) 92.1 (1) 16 (5) 104.5 (1) 0.46 4e-05 ***

 

Long Term Potentiation

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Variables shown are: LTP vs PTP, amplitude ratio, Reduction in potentiation from immediately after theta-burst stimulation to one hour later, fEPSP amplitude ratio. LTP based on amplitude, Long term potentiation, ratio of amplitudes of fEPSPs in test pathway and control pathway.

 

Variable Units Wildtype slices (animals) Wildtype mean (SEM) Mutant slices (animals) Mutant mean (SEM) P(animals) P(mutation)
LTP vs PTP, amplitude ratio % 16 (5) 79 (1.3) 16 (5) 78.8 (1.5) 0.95 0.84
LTP based on amplitude % 16 (5) 173.7 (7.7) 16 (5) 200.3 (8.8) 0.25 0.083

Hippocampal brain slices summarised by genotype, followed by a list of the animals from which they were prepared.

View experimental protocols.

 

Hippocampal Slice Electrophysiology Cohort Summary

GenotypeCount
WT5
mutant5

 

Individual Mice

Mouse IDGenotype
wBbf4efb9e7WT
wAe263651eeWT
wB919a84dd8WT
wAf82e39cd2WT
wA7f46106f6WT
mAbf4efb9e7mutant
mBf82e39cd2mutant
mB7f46106f6mutant
mA919a84dd8mutant
mBe263651eemutant

Field EPSP (Maximum)

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBf82e39cd2 mutant 5 52A f82e39cd2_slices_4-5-6-7.zip
mBf82e39cd2 mutant 6 46B f82e39cd2_slices_4-5-6-7.zip
mBf82e39cd2 mutant 7 62B f82e39cd2_slices_4-5-6-7.zip
mB7f46106f6 mutant 5 62A 7f46106f6_slices_4-5-6-7.zip
mB7f46106f6 mutant 6 46B 7f46106f6_slices_4-5-6-7.zip
mA919a84dd8 mutant 1 12A 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 2 52A 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 3 46B 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 4 55A 919a84dd8_slices_4-5-6-7.zip
mAbf4efb9e7 mutant 1 46A bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 2 62A bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 3 12B bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 4 26A bf4efb9e7_slices_4-5-6-7.zip
mBe263651ee mutant 5 41A e263651ee_slices_4-5-6-7.zip
mBe263651ee mutant 6 12B e263651ee_slices_4-5-6-7.zip
mBe263651ee mutant 7 52B e263651ee_slices_4-5-6-7.zip
wAf82e39cd2 WT 1 15A f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 2 52A f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 3 46B f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 4 55A f82e39cd2_slices_4-5-6-7.zip
wA7f46106f6 WT 1 46A 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 2 42A 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 3 12B 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 4 58B 7f46106f6_slices_4-5-6-7.zip
wB919a84dd8 WT 5 62A 919a84dd8_slices_4-5-6-7.zip
wB919a84dd8 WT 6 38B 919a84dd8_slices_4-5-6-7.zip
wB919a84dd8 WT 7 62B 919a84dd8_slices_4-5-6-7.zip
wBbf4efb9e7 WT 5 54A bf4efb9e7_slices_4-5-6-7.zip
wBbf4efb9e7 WT 6 46B bf4efb9e7_slices_4-5-6-7.zip
wBbf4efb9e7 WT 7 62B bf4efb9e7_slices_4-5-6-7.zip
wAe263651ee WT 1 15A e263651ee_slices_1-2-3-x.zip
wAe263651ee WT 3 46B e263651ee_slices_1-2-3-x.zip

Paired Pulse Facilitation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBf82e39cd2 mutant 5 52A f82e39cd2_slices_4-5-6-7.zip
mBf82e39cd2 mutant 6 46B f82e39cd2_slices_4-5-6-7.zip
mBf82e39cd2 mutant 7 62B f82e39cd2_slices_4-5-6-7.zip
mB7f46106f6 mutant 5 62A 7f46106f6_slices_4-5-6-7.zip
mB7f46106f6 mutant 6 46B 7f46106f6_slices_4-5-6-7.zip
mA919a84dd8 mutant 1 12A 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 2 52A 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 3 46B 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 4 55A 919a84dd8_slices_4-5-6-7.zip
mAbf4efb9e7 mutant 1 46A bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 2 62A bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 3 12B bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 4 26A bf4efb9e7_slices_4-5-6-7.zip
mBe263651ee mutant 5 41A e263651ee_slices_4-5-6-7.zip
mBe263651ee mutant 6 12B e263651ee_slices_4-5-6-7.zip
mBe263651ee mutant 7 52B e263651ee_slices_4-5-6-7.zip
wAf82e39cd2 WT 1 15A f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 2 52A f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 3 46B f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 4 55A f82e39cd2_slices_4-5-6-7.zip
wA7f46106f6 WT 1 46A 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 2 42A 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 3 12B 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 4 58B 7f46106f6_slices_4-5-6-7.zip
wB919a84dd8 WT 5 62A 919a84dd8_slices_4-5-6-7.zip
wB919a84dd8 WT 6 38B 919a84dd8_slices_4-5-6-7.zip
wB919a84dd8 WT 7 62B 919a84dd8_slices_4-5-6-7.zip
wBbf4efb9e7 WT 5 54A bf4efb9e7_slices_4-5-6-7.zip
wBbf4efb9e7 WT 6 46B bf4efb9e7_slices_4-5-6-7.zip
wBbf4efb9e7 WT 7 62B bf4efb9e7_slices_4-5-6-7.zip
wAe263651ee WT 1 15A e263651ee_slices_1-2-3-x.zip
wAe263651ee WT 3 46B e263651ee_slices_1-2-3-x.zip

Long Term Potentiation

Mouse IDGenotypeSlice NumberRecording ChannelFile Name
mBf82e39cd2 mutant 5 52A f82e39cd2_slices_4-5-6-7.zip
mBf82e39cd2 mutant 6 46B f82e39cd2_slices_4-5-6-7.zip
mBf82e39cd2 mutant 7 62B f82e39cd2_slices_4-5-6-7.zip
mB7f46106f6 mutant 5 62A 7f46106f6_slices_4-5-6-7.zip
mB7f46106f6 mutant 6 46B 7f46106f6_slices_4-5-6-7.zip
mA919a84dd8 mutant 1 12A 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 2 52A 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 3 46B 919a84dd8_slices_1-2-3-x.zip
mA919a84dd8 mutant 4 55A 919a84dd8_slices_4-5-6-7.zip
mAbf4efb9e7 mutant 1 46A bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 2 62A bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 3 12B bf4efb9e7_slices_1-2-3-x.zip
mAbf4efb9e7 mutant 4 26A bf4efb9e7_slices_4-5-6-7.zip
mBe263651ee mutant 5 41A e263651ee_slices_4-5-6-7.zip
mBe263651ee mutant 6 12B e263651ee_slices_4-5-6-7.zip
mBe263651ee mutant 7 52B e263651ee_slices_4-5-6-7.zip
wAf82e39cd2 WT 1 15A f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 2 52A f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 3 46B f82e39cd2_slices_1-2-3-x.zip
wAf82e39cd2 WT 4 55A f82e39cd2_slices_4-5-6-7.zip
wA7f46106f6 WT 1 46A 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 2 42A 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 3 12B 7f46106f6_slices_1-2-3-x.zip
wA7f46106f6 WT 4 58B 7f46106f6_slices_4-5-6-7.zip
wB919a84dd8 WT 5 62A 919a84dd8_slices_4-5-6-7.zip
wB919a84dd8 WT 6 38B 919a84dd8_slices_4-5-6-7.zip
wB919a84dd8 WT 7 62B 919a84dd8_slices_4-5-6-7.zip
wBbf4efb9e7 WT 5 54A bf4efb9e7_slices_4-5-6-7.zip
wBbf4efb9e7 WT 6 46B bf4efb9e7_slices_4-5-6-7.zip
wBbf4efb9e7 WT 7 62B bf4efb9e7_slices_4-5-6-7.zip
wAe263651ee WT 1 15A e263651ee_slices_1-2-3-x.zip
wAe263651ee WT 3 46B e263651ee_slices_1-2-3-x.zip

Data Files

File NameSizeChecksum (SHA256)
e263651ee_slices_4-5-6-7.zip5224641532e20cabc928330d0f0387957dc555f76b45abb7edb4c95b4163e93b866910a5
7f46106f6_slices_1-2-3-x.zip356640982e186fe49dbcf9743900c0e1eadd888203d7349721a890811f962387b02fc2a4
f82e39cd2_slices_1-2-3-x.zip31721332f7ebe13caea9ce1d951d5e3bd433fe46a313dccd694d1b83b86a794395722374
e263651ee_slices_1-2-3-x.zip305310338568241109179b824bc73e10320b1a7a5939eb65f72bb7e5e30c2d485aaf8b23
919a84dd8_slices_1-2-3-x.zip32573985064ccb951dbdee7daa32ba6d764c71c3887c1dbc59fccd5b8aed594034bd210e
919a84dd8_slices_4-5-6-7.zip4743657942baf7113ed6d4178fbfc561b3782b03346dae0e22d6e4a81f2800067d884bc8
f82e39cd2_slices_4-5-6-7.zip4782879603e4e290f309f5f9926e7144a69d58adfb60680637eb84f2146ff3879ba84054
bf4efb9e7_slices_1-2-3-x.zip346281608f1a5b9b9c7ce6918755c9d4bd88620904a48efec67ec852587b07c93fbf5ebd
7f46106f6_slices_4-5-6-7.zip49339916c5f5d6cf4b0ca5a14aa2e9a86c5c2454f6dab4dab53534e18c34628d5b5d28f9
bf4efb9e7_slices_4-5-6-7.zip48938370da7a9ac2eb06636ee4e929429a0586b408d5b7076ed85dc72bc7aec4828aa82b
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