G2C::Genetics

Mutation

Generation of PSD-95-PDZ3 mutant mice described in Migaud et al (1998)

Overview

Mutant mice showed large overall behavioural difference from wildtypes, with 11 of 16 behaviour variables significantly impacted by this mutation. In the elevated plus maze task, four behavioural variables of five were significantly impacted in mutants. In the open field/novel object exploration task, two behavioural variables of two were significantly impacted in mutants. In the rotarod task, two behavioural variables of three were significantly impacted in mutants. In the fear learning task, one behavioural variable, fear learning trial effect, was significantly increased in mutants. In the contextual memory task, one behavioural variable, contextual memory mean, was significantly decreased in mutants. In the cued memory task, one behavioural variable, cued memory change, was significantly decreased in mutants. Definitions of variables may be found below.

The G2CMine data warehouse provides cohort summaries and individual mouse observations from the PSD-95-PDZ3 knock-out line phenotyping.

 

Variables shown are: EPM total distance, Total distance (cm) travelled in any arm or central zone of the EPM. EPM max speed, Maximum speed (cm/s) travelled in any arm or central zone of the EPM. EPM % time in open, Percentage of time in the open or closed arms of the EPM spent in open arms. EPM time in centre, Total time (s) spent in the central zone of the EPM. EPM max speed, open vs closed, Difference between the maximum speed (cm/s) observed in the open arms and the closed arms of the EPM. OF, NOE total distance, Total distance travelled (log₁₀ cm) during initial exposure to the open field and in presence of the novel object. NOE vs OF distance travelled, Difference in distance travelled (cm) in presence of the novel object and during initial exposure to open field. RR naive fall time, Fall time on accelerating rotarod (log₁₀ s), naive performance in session 1. RR learning, Learning on rotarod, measured as increase in fall time per trial (s/trial) in session 1. RR memory, Memory on rotarod, measured as excess fall time at middle of session 2 relative to middle of session 1. Fear learning, trial effect, Fear learning, measured as extra % time freezing before third trial compared to % time freezing before first trial. Fear learning, tone effect, Fear learning, measured as increase in % time freezing due to third tone compared to increase in % time freezing due to first tone. Contextual memory, mean, Contextual memory, measured as difference in % time freezing during first 120 s re-exposure to the box compared to first 120 s in the box on previous day. Contextual memory, change, Contextual memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the box. Cued memory, mean, Cued memory, measured as increase in % time spent freezing during 120 s of tone re-exposure compared to increase in % time spent freezing during initial tone on previous day. Cued memory, change, Cued memory, measured as increase in % time spent freezing from first time bin of 30 s to fourth bin of 30 s during 120 s re-exposure to the tone.

 

Variable	Units	Wildtype M (n=16)	Wildtype F (n=11)	Mutant M (n=16)	Mutant F (n=13)	P(sex×mutation)	P(mutation)
EPM total distance	cm	1176 (60)	1287 (99)	1003 (106)	1086 (83)	0.87	0.042 *
EPM max speed	cm/s	25 (1)	27.1 (2.2)	22 (1.9)	22.5 (1.1)	0.63	0.025 *
EPM % time in open	%	22.4 (4)	21.1 (8.2)	53.4 (8.1)	66.5 (6.4)	0.3	0.0000012 ***
EPM time in centre	s	56.6 (5.3)	58.2 (6.9)	31.3 (6.7)	42.3 (5.5)	0.45	0.0011 **
EPM max speed, open vs closed	cm/s	-4.6 (1.3)	-5.5 (2.8)	-2.9 (2.4)	-0.1 (2)	0.39	0.13
OF, NOE total distance	log10 cm	3.63 (0.03)	3.74 (0.02)	3.46 (0.05)	3.4 (0.04)	0.038 *	0.00000012 ***
NOE vs OF distance travelled	cm	-1270 (170)	-1150 (230)	-830 (130)	-760 (120)	0.88	0.012 *
RR naive fall time	log10 s	1.41 (0.1)	1.5 (0.12)	1.05 (0.05)	1.35 (0.06)	0.24	0.0024 **
RR learning	s/trial	7.5 (1.2)	7.7 (1.7)	2.4 (0.5)	2.6 (1)	0.99	0.000018 ***
RR memory	s	17.7 (5)	22.6 (7.5)	10.3 (4.5)	15.4 (4.9)	0.98	0.18
Fear learning, trial effect	% freezing	43.4 (5.7)	39 (7.5)	67.6 (5.9)	76.4 (5.2)	0.29	0.0000089 ***
Fear learning, tone effect	% freezing	6.2 (3.6)	1.5 (5.9)	2.3 (5.8)	-7.1 (10.1)	0.72	0.37
Contextual memory, mean	% freezing	38.5 (4.8)	29 (5.8)	23.1 (3.9)	13.8 (5.7)	0.99	0.0033 **
Contextual memory, change	% freezing	14.7 (5.3)	-4.1 (11.9)	2 (6.2)	-18 (7.6)	0.94	0.085
Cued memory, mean	% freezing	-1.1 (4.6)	10 (8.1)	-7.7 (6)	-1.7 (11.5)	0.74	0.25
Cued memory, change	% freezing	0.7 (7.2)	10.1 (6.8)	-10 (5.5)	-9 (5.8)	0.53	0.031 *

Overview

The G2CMine data warehouse provides slice group summaries and individual mouse observations from the PSD-95-PDZ3 knock-out line phenotyping.