G2C::Genetics

TNIK is required for postsynaptic and nuclear signalling pathways and cognitive function

Marcello P. Coba1,2*, Noboru H. Komiyama1,3*, Jess Nithianantharajah1,3*, Maksym V. Kopanitsa1,4, Tim Indersmitten5, Nathan G. Skene1, Ellie J. Tuck1,4, David G. Fricker1,4, Kathryn A. Elsegood1,3, Lianne E. Stanford1, Nurudeen Afinowi1,4, Lisa M. Saksida6, Timothy J. Bussey6, Thomas J. O'Dell7 and Seth G.N. Grant1,3,6

Author email: Seth.Grant@ed.ac.uk   * - These authors contributed equally to this work

  1. Genes to Cognition Programme, The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire, CB10 1SA, UK
  2. Zilkha Neurogenetic Institute, Department of Psychiatry and Behavioral Sciences, University of Southern California, Los Angeles, California, 90089, USA
  3. Centre for Clinical Brain Sciences and Centre for Neuroregeneration, The University of Edinburgh, Chancellors Building, 47 Little France Crescent, Edinburgh EH16 4SB, UK
  4. Synome Ltd, Babraham Research Campus, Cambridge, CB22 3AT, UK
  5. Interdepartmental PhD Program for Neuroscience, UCLA, Los Angeles, California 90095, USA
  6. Department of Experimental Psychology, University of Cambridge, UK; The MRC and Wellcome Trust Behavioural and Clinical Neuroscience Institute, University of Cambridge, Downing St., Cambridge CB2 3EB, UK
  7. Department of Physiology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA

 

 

Traf2 and NcK interacting Kinase (TNiK) contains serine-threonine kinase and scaffold domains and has been implicated in cell proliferation and glutamate receptor regulation in vitro. Here we report its role in vivo using mice carrying a knockout mutation.

TNiK binds protein complexes in the synapse linking it to the NMDA receptor (NMDAR) via AKAP9. NMDAR and metabotropic receptors bidirectionally regulate TNiK phosphorylation and TNiK was required for AMPA expression and synaptic function. TNiK also organises nuclear complexes and in the absence of TNiK, there was a marked elevation in GSK3Β and phosphorylation levels of its cognate phosphorylation sites on NeuroD1 with alterations in Wnt pathway signalling.

We observed impairments in dentate gyrus neurogenesis in TNiK knockout mice and cognitive testing using the touchscreen apparatus revealed impairments in pattern separation on a test of spatial discrimination. Object-location paired associates learning, which is dependent on glutamatergic signalling was also impaired. Additionally, TNiK knockout mice displayed hyperlocomotor behavior that could be rapidly reversed by GSK3Β inhibitors, indicating the potential for pharmacological rescue of a behavioral phenotype.

These data establish TNiK as a critical regulator of cognitive functions and suggest it may play a regulatory role in diseases impacting on its interacting proteins and complexes.

Supplementary Figure 1. Generation of AKAP9 mutant mice

A mouse embryonic stem (ES) cell line (XP0050, strain 129/Ola) with an insertional mutation in Akap9 was obtained from Sanger Institute Gene Trap Resource (SIGTR - sanger.ac.uk/PostGenomics/genetrap/). The insertional mutation in XP0050 by the gene-trapping vector, pGT0lxr, was designed to create an in-frame fusion between the 5' exons of the trapped gene and a reporter, Β-geo (a fusion of Β-galactosidase and neomycin phosphotransferase II) occurred in intron 14-15 (Transcript: Akap9-001 (ENSMUST00000044492) Ensembl release 56). Thus, the gene-trapped locus is predicted to yield a fusion transcript containing exons 1-14 of Akap9 and Β-geo. Integration of the gene-trapping vector was confirmed by RT-PCR. A 217bp product was amplified from the gene trap clone cDNA using primer x (GAAGCTGTCTAAGAGAGTGTG) that hybridises the sequence encoded by the part of exon 14 with reverse Primer z (GATCCTCTAGAGTCCAGATCTG) within the Β-geo cassette. This gene trap ES cell clone was injected into C57/BL6 blastocysts to create chimeric mice, which were bred with 129S5 mice to generate heterozygous (+/–) Akap9 mutant mice. Those F1 heterozygous mice had been backcrossed with 129S5 mice for 1-2 times before being used for intercrossing.

TNiK Supplementary Figure 1

TNiK Supplementary Figure 1
Enlarge this image (578 x 900)

A. Gene trap vector for the generation of AKAP9 knockout mice. Linear structure of AKAP9 protein domain (top diagram). AKAP9 is a 48 exon protein. The gene-trapping vector, pGT0lxr, is inserted between exon14 and 15. Primers used for genotyping (see supplementary methods) are shown (bottom diagram). SA, splice acceptor; pA, polyadenylation signal; Β-geo, fusion of Β-galactosidase and neomycin phosphotransferase II.

B. Electrophoresis gel image showing confirmation by RTPCR that trap is correctly inserted.

C. RT-PCR genotyping. Homozygote (-/-) product is 205bp larger than wt (+/+) product.

D. PCR genotyping of targeted AKAP9-/- mice using a common forward primer, a, and reverse primers b and y to amplify the wt and mutant alleles respectively.

Supplementary Table 1. Hippocampal mRNA expression profile of TNiK-/- mice

Shown are the 200 most significant genes from the mRNA expression profiling, the whole genome array results are downloadable.

Download whole genome microarray results

Gene SymbolGene DescriptionEnsembl Gene IDEntrez Gene IDAverage Expressionlog Fold ChangeP ValueAdjusted P ValuetGenomic LocationProbe LocationRefSeq transcriptsProbe id
Spata13Spermatogenesis associated 13ENSMUSG000000219909.20-0.3576.44E-091.49E-04-7.17chr14:61383157:61383206:+3pUTRXM_923227 XM_901902ILMN_2751037
CortCortistatinENSMUSG000000289712584538.400.3783.32E-083.84E-046.69chr4:148499369:148499418:-CDSNM_007745ILMN_1226607
BC050868Mus musculus adult male cerebellum cDNA, RIKEN full-length enriched library, clone:1500031A17 product:TRAF2 AND NCK INTERACTING KINASE, SPLICE VARIANT 8 homolog [Homo sapiens], full insert sequence.ENSMUSG0000002769210.07-0.4036.85E-085.28E-04-6.47chr3:28569142:28569191:+3pUTRXM_001473621 XM_001473602 XM_001474909 XM_001474897ILMN_2524251
C4bComplement component 4B (Childo blood group)ENSMUSG000000734182122858.68-0.3353.40E-061.66E-02-5.32chr17:34866134:34866183:-CDSNM_009780 NM_011413 XM_973028 XM_972987 XM_973068ILMN_1215092
Scara3Scavenger receptor class A, member 3ENSMUSG0000003446326899610.26-0.2835.07E-061.66E-02-5.20chr14:66538293:66538342:-3pUTRNM_172604ILMN_2706268
Slc30a3Solute carrier family 30 (zinc transporter), member 3ENSMUSG000000291512278412.22-0.2835.20E-061.66E-02-5.19chr5:31388548:31388597:-3pUTRILMN_2521965
C4bComplement component 4B (Childo blood group)ENSMUSG00000015451788298.53-0.2885.82E-061.66E-02-5.15chr17:34865420:34865469:-CDSNM_009780 NM_011413 XM_973028 XM_972987 XM_973068ILMN_3049559
Tcirg1T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3ENSMUSG000000017502582037.57-0.1036.47E-061.66E-02-5.12chr19:3903583:3903632:-CDSNM_016921ILMN_2643879
Hn1Hematological and neurological expressed sequence 1ENSMUSG000000207376920212.59-0.1966.80E-061.66E-02-5.11chr11:115358689:115358738:-3pUTRNM_008258ILMN_2914744
Cox6a2Cytochrome c oxidase, subunit VI a, polypeptide 2ENSMUSG000000307852582898.530.3367.19E-061.66E-025.09chr7:135349162:135349211:-3pUTRNM_009943ILMN_2629581
9130213B05RikRIKEN cDNA 9130213B05 geneENSMUSG0000003498123144011.200.4371.20E-052.23E-024.93chr5:92052926:92052975:+3pUTRNM_145562ILMN_2756704
Ebf3Early B-cell factor 3ENSMUSG000000104767.950.1271.22E-052.23E-024.93chr7:144385579:144385628:-3pUTRNM_001113414 NM_001113415 NM_010096ILMN_2422615
RhagRhesus blood group-associated A glycoproteinENSMUSG00000023926164287.55-0.0841.25E-052.23E-02-4.92chr17:40971620:40971669:+CDSNM_011269ILMN_2745515
RabggtbRAB geranylgeranyl transferase, b subunitENSMUSG000000389753383607.74-0.1461.64E-052.70E-02-4.84chr3:153573046:153573095:-3pUTRILMN_2703263
Myo5bMyosin VBENSMUSG00000025885179199.94-0.4232.07E-053.19E-02-4.77chr18:74930039:74930088:+CDSNM_201600ILMN_2539489
A830018L16RikRIKEN cDNA A830018L16 gene690358.72-0.2102.23E-053.23E-02-4.75chr1:11588290:11588339:+3pUTRILMN_1213044
Nat8lN-acetyltransferase 8-likeENSMUSG00000048142562089.720.2842.59E-053.53E-024.70chr5:34345517:34345566:+3pUTRNM_001001985ILMN_2594039
GraspGRP1 (general receptor for phosphoinositides 1)-associated scaffold proteinENSMUSG000000005316769211.35-0.2162.84E-053.64E-02-4.67chr15:101063106:101063155:+3pUTRNM_019518ILMN_2596396
Usp52Ubiquitin specific peptidase 52ENSMUSG00000005682745598.57-0.2543.42E-053.66E-02-4.61chr10:127758185:127758234:+3pUTRNM_133992ILMN_2790392
Bat2dBAT2 domain containing 1ENSMUSG00000040225719837.820.3053.67E-053.66E-024.59chr1:164640509:164640558:-CDSNM_001081290ILMN_1239599
LumLumicanENSMUSG000000364468.24-0.2793.72E-053.66E-02-4.59chr10:97034583:97034632:+CDSNM_008524ILMN_3001540
Tle1Transducin-like enhancer of split 1, homolog of Drosophila E(spl)ENSMUSG000000083052406678.62-0.2754.25E-053.66E-02-4.55chr4:71819008:71819057:-3pUTRILMN_1245089
IfnzInterferon zetaENSMUSG00000073810747537.59-0.0894.58E-053.66E-02-4.52chr4:88428166:88428215:+5pUTRNM_197889ILMN_3021483
B4galnt4Beta-1,4-N-acetyl-galactosaminyl transferase 43306718.51-0.1784.67E-053.66E-02-4.52chr7:148254691:148254740:+CDSILMN_2540866
Zzz3Zinc finger, ZZ domain containing 3ENSMUSG00000039068154817.610.0934.68E-053.66E-024.52chr3:152085636:152085685:+5pUTRXR_001912 XR_032051 NM_198416 NM_001080755ILMN_2424408
Scara5Scavenger receptor class A, member 5 (putative)ENSMUSG000000220327.55-0.0884.83E-053.66E-02-4.51chr14:66383255:66383304:+3pUTRNM_028903ILMN_3008068
Htr1f5-hydroxytryptamine (serotonin) receptor 1FENSMUSG000000507832276827.860.1824.96E-053.66E-024.50chr16:64924829:64924878:-3pUTRNM_008310ILMN_2933261
Eltd1EGF, latrophilin seven transmembrane domain containing 1ENSMUSG000000391671405777.960.1624.97E-053.66E-024.50chr3:151207706:151207755:+3pUTRNM_133222ILMN_1224540
Stx4aSyntaxin 4A (placental)ENSMUSG000000308052288808.500.1585.49E-053.66E-024.47chr7:134992177:134992181:+,chr7:134992265:134992309:+3pUTRNM_009294ILMN_2822053
2810006K23RikRIKEN cDNA 2810006K23 geneENSMUSG00000047635126617.580.0735.53E-053.66E-024.46chr5:124790696:124790745:+CDSILMN_2546984
Mtap1aMicrotubule-associated protein 1 AENSMUSG00000027254741438.140.3155.66E-053.66E-024.46chr2:121126148:121126197:+CDSNM_032393ILMN_2646070
EG434171Predicted gene, EG434171ENSMUSG00000060565764867.69-0.1156.06E-053.66E-02-4.44chr7:39303890:39303939:-CDSNM_001013810ILMN_2879144
PtprbProtein tyrosine phosphatase, receptor type, BENSMUSG00000020154273578.100.3886.19E-053.66E-024.43chr10:115820928:115820977:+CDSNM_029928ILMN_2591731
CortCortistatinENSMUSG000000289712383238.700.3496.39E-053.66E-024.42chr4:148499316:148499365:-3pUTRNM_007745ILMN_2955725
2310007H09RikRIKEN cDNA 2310007H09 geneENSMUSG000000309462582938.60-0.2196.40E-053.66E-02-4.42chr7:139897331:139897380:+CDSNM_029609ILMN_2600720
AK021368Mus musculus 0 day neonate eyeball cDNA, RIKEN full-length enriched library, clone:E130102H24 product:hypothetical protein, full insert sequence.181058.45-0.3136.54E-053.66E-02-4.41chr4:101019192:101019241:-3pUTRXM_001472210 XM_992447ILMN_2647694
Ddrgk1DDRGK domain containing 1ENSMUSG0000006829011.57-0.2076.54E-053.66E-02-4.41chr2:130479850:130479899:-CDSNM_029832ILMN_2874128
Parp2Poly (ADP-ribose) polymerase family, member 2ENSMUSG00000036023115469.96-0.1546.61E-053.66E-02-4.41chr14:51440785:51440834:+CDSNM_009632ILMN_1226731
Gm1040Gene model 1040, (NCBI)ENSMUSG000000015694338647.62-0.0956.80E-053.66E-02-4.40chr5:29762076:29762125:+CDSNM_001033457ILMN_2537214
BcorBCL6 interacting corepressorENSMUSG00000040363687208.08-0.1776.89E-053.66E-02-4.40chrX:11614985:11615034:-CDSNM_175045 NM_029510 NM_175044 NM_175046ILMN_1228091
Ankrd11Ankyrin repeat domain 11ENSMUSG000000355698.160.3866.89E-053.66E-024.40chr8:125417578:125417627:-CDSNM_001081379ILMN_2565729
Shmt2Serine hydroxymethyltransferase 2 (mitochondrial)8.070.1187.03E-053.66E-024.39chr10:126954204:126954253:-3pUTRNM_028230ILMN_1224942
Bat2dBAT2 domain containing 1ENSMUSG000000402257.990.4327.12E-053.66E-024.39chr1:164640508:164640557:-CDSNM_001081290ILMN_1241229
Irx3Iroquois related homeobox 3 (Drosophila)ENSMUSG00000031734748957.61-0.0787.24E-053.66E-02-4.38chr8:94322817:94322866:-CDSNM_008393ILMN_2776909
Psen1Presenilin 1ENSMUSG00000019969582348.65-0.3087.48E-053.66E-02-4.37chr12:85074933:85074982:+CDSNM_008943ILMN_1214811
Slc7a7Solute carrier family 7 (cationic amino acid transporter, y+ system), member 7ENSMUSG000000009582181037.66-0.1017.61E-053.66E-02-4.37chr14:54992648:54992697:-CDSNM_011405ILMN_2723826
BccipBRCA2 and CDKN1A interacting proteinENSMUSG000000309836760610.59-0.2537.69E-053.66E-02-4.36chr7:140912451:140912500:+CDSNM_025392ILMN_2759920
C330006K01RikRIKEN cDNA C330006K01 geneENSMUSG000000396238.400.1088.18E-053.66E-024.34chr5:142953681:142953730:+3pUTRXM_001480714 XM_001480711 XM_001480708 NM_172725ILMN_2752206
NrasNeuroblastoma ras oncogene1415810.94-0.3158.41E-053.66E-02-4.33chr3:102871584:102871633:+3pUTRNM_010937ILMN_2837543
Ppp2r4Protein phosphatase 2A, regulatory subunit B (PR 53)ENSMUSG000000395152168818.280.2098.41E-053.66E-024.33chr2:30303115:30303164:+3pUTRNM_138748ILMN_2755399
Acpl2Acid phosphatase-like 2ENSMUSG000000435879.59-0.2858.59E-053.66E-02-4.33chr9:96723879:96723928:-3pUTRNM_153420ILMN_1254634
2810474O19RikRIKEN cDNA 2810474O19 geneENSMUSG00000032712672467.810.2178.68E-053.66E-024.32chr6:149274925:149274974:+CDSNM_026054ILMN_2596077
Sqstm1Sequestosome 1ENSMUSG000000158371804811.70-0.3508.85E-053.66E-02-4.32chr11:50014169:50014218:-3pUTRNM_011018ILMN_2710705
Fhl1Four and a half LIM domains 1ENSMUSG000000230921461511.02-0.3198.89E-053.66E-02-4.32chrX:54046293:54046342:+3pUTRNM_010211 NM_001077361 NM_001077362ILMN_2713285
Dennd5aDENN/MADD domain containing 5AENSMUSG000000359011595111.05-0.2249.32E-053.66E-02-4.30chr7:117037678:117037727:-3pUTRNM_021494 XM_001477127ILMN_3107607
LcatLecithin cholesterol acyltransferaseENSMUSG00000035237168168.380.1649.36E-053.66E-024.30chr8:108463676:108463725:-CDSNM_008490ILMN_2798400
Hmgn2High mobility group nucleosomal binding domain 2ENSMUSG0000000303810.90-0.3389.43E-053.66E-02-4.30chr4:133521188:133521236:-3pUTRXM_001478555ILMN_1238360
HmbsHydroxymethylbilane synthaseENSMUSG00000073552701208.72-0.2229.44E-053.66E-02-4.30chr9:44145454:44145503:-CDSXM_915480 NM_013551 XM_888318 NM_001110251ILMN_2657124
Lmo3LIM domain only 3ENSMUSG000000302261309010.91-0.2739.54E-053.66E-02-4.29chr6:138313349:138313398:-3pUTRNM_207222ILMN_3062075
Flt1FMS-like tyrosine kinase 1ENSMUSG00000029648142549.720.4449.65E-053.66E-024.29chr5:148373789:148373838:-3pUTRNM_010228ILMN_1227926
Zfp821Zinc finger protein 821ENSMUSG000000317283198018.36-0.3009.66E-053.66E-02-4.29chr8:112246886:112246935:+CDSNM_029468ILMN_2693387
Pdlim3PDZ and LIM domain 3238347.790.1189.92E-053.70E-024.28chr8:47004761:47004810:+3pUTRNM_016798ILMN_2885990
SrcRous sarcoma oncogeneENSMUSG000000276462453087.71-0.0940.0001017843.74E-02-4.27chr2:157295241:157295257:+,chr2:157295489:157295521:+CDSNM_001025395 NM_009271ILMN_2677529
Ankra2Ankyrin repeat, family A (RFXANK-like), 2ENSMUSG00000021661264288.57-0.2480.0001040983.76E-02-4.27chr13:99043230:99043279:+3pUTRILMN_1243090
Hmgn2High mobility group nucleosomal binding domain 2ENSMUSG0000000303810.94-0.4260.0001123713.86E-02-4.24chr4:133521180:133521229:-3pUTRXM_001473104 XM_001477164 XM_001476615 XM_891577 NM_016957 XM_001478560 XM_001479392ILMN_1237216
Prss23Protease, serine, 23764537.600.0800.0001142913.86E-024.24chr7:96656349:96656398:-3pUTRNM_029614ILMN_1246127
Klhl6Kelch-like 6 (Drosophila)ENSMUSG000000430083824067.580.0790.0001149853.86E-024.23chr16:19953586:19953635:-CDSNM_183390ILMN_1260233
Slco1c1Solute carrier organic anion transporter family, member 1c1ENSMUSG00000030235120339.580.3780.0001167133.86E-024.23chr6:141517967:141518016:+CDSNM_021471ILMN_1242173
Cog56 days neonate head cDNA, RIKEN full-length enriched library, clone:5430405C01 product:PUTATIVE 13 S GOLGI TRANSPORT COMPLEX 90KD SUBUNIT BRAIN-SPECIFIC ISOFORM homolog (Fragment).ENSMUSG000000359332263528.63-0.1960.0001194923.86E-02-4.22chr12:32604642:32604642:+,chr12:32605419:32605467:+CDSILMN_2523980
Krt14Keratin 14ENSMUSG000000455452590277.570.0750.0001200373.86E-024.22chr11:100064523:100064572:-3pUTRNM_016958ILMN_2722616
Fbxl6F-box and leucine-rich repeat protein 6ENSMUSG00000022559769378.80-0.2120.0001202433.86E-02-4.22chr15:76367007:76367056:-CDSNM_013909ILMN_1216206
SqleSqualene epoxidaseENSMUSG000000223516698810.56-0.3580.0001238623.86E-02-4.21chr15:59161327:59161376:+CDSNM_009270ILMN_2737163
InhbaInhibin beta-AENSMUSG00000041324116108.110.2080.0001240353.86E-024.21chr13:16118809:16118858:+CDSNM_008380ILMN_2868220
LOC380728similar to potassium voltage-gated channel,ENSMUSG000000353553807287.59-0.0970.0001271933.86E-02-4.20chr11:100605448:100605497:-CDSNM_001081194ILMN_2534442
Pkd1l2Polycystic kidney disease 1 like 2766457.60-0.0850.0001274423.86E-02-4.20chr8:119547574:119547623:-3pUTRILMN_2550168
Esr1Estrogen receptor 1 (alpha)ENSMUSG00000019768740907.610.0870.0001277343.86E-024.20chr10:5342933:5342982:-3pUTRNM_007956ILMN_2726412
CpdCarboxypeptidase DENSMUSG00000020841710837.670.1220.0001285083.86E-024.20chr11:76595547:76595596:-3pUTRNM_007754 XM_001476801ILMN_2727598
Lypd6LY6/PLAUR domain containing 6ENSMUSG00000050447126618.420.2300.000130453.87E-024.19chr2:50047963:50048012:+3pUTRNM_177139ILMN_2729745
Pfdn5Prefoldin 5ENSMUSG0000000128927120912.67-0.2080.0001340273.90E-02-4.19chr15:102158963:102159012:+CDSNM_027044ILMN_2671747
9130011J15RikRIKEN cDNA 9130011J15 gene10003885910.60-0.2810.0001348263.90E-02-4.18chr8:75090014:75090063:-3pUTRNM_172396ILMN_2593484
Atp6v1b1ATPase, H+ transporting, lysosomal V1 subunit B1ENSMUSG000000062691041117.65-0.0740.0001420944.04E-02-4.17chr6:83708597:83708646:+3pUTRNM_134157ILMN_2879910
Epb4.1l5Erythrocyte protein band 4.1-like 52263527.53-0.0680.0001431274.04E-02-4.17chr1:121491758:121491807:-3pUTRNM_001113416ILMN_2586966
Cln3Ceroid lipofuscinosis, neuronal 3, juvenile (Batten, Spielmeyer-Vogt disease)ENSMUSG000000307202456848.85-0.2070.0001502284.12E-02-4.15chr7:133715628:133715677:-3pUTRXM_001479838 NM_009907ILMN_1214952
HephHephaestinENSMUSG000000312092248247.95-0.1790.0001518924.12E-02-4.15chrX:93769002:93769051:+CDSNM_010417ILMN_2734252
C4bComplement component 4B (Childo blood group)ENSMUSG00000073418679787.58-0.0760.0001522644.12E-02-4.15chr17:34871083:34871132:-CDSNM_009780 XM_973028 XM_972987 XM_973068 XM_001475152ILMN_2632567
Vmn2r111Vomeronasal 2, receptor 111ENSMUSG000000518772108767.55-0.0730.0001552854.12E-02-4.14chr17:22755892:22755941:+CDSNM_001104575ILMN_2530517
Sec22aSEC22 vesicle trafficking protein homologe A (S. cerevisiae)ENSMUSG000000344733301777.600.0540.0001582084.12E-024.13chr16:35311501:35311550:-3pUTRNM_133704ILMN_2742128
Arid4aAT rich interactive domain 4A (RBP1-like)ENSMUSG000000481188.090.3430.0001583144.12E-024.13chr12:72164095:72164144:+CDSNM_001081195ILMN_1239608
Rps6kl1Ribosomal protein S6 kinase-like 1ENSMUSG0000001923510.68-0.2540.000158414.12E-02-4.13chr12:86476728:86476777:-3pUTRNM_146244ILMN_2649333
Lyrm2LYR motif containing 2746267.540.0690.0001691064.27E-024.11chr4:32888978:32889027:+3pUTRNM_175364ILMN_2732585
Yif1bYip1 interacting factor homolog B (S. cerevisiae)ENSMUSG000000305881000394969.72-0.2660.0001703654.27E-02-4.11chr7:30030935:30030984:+CDSNM_001110201 NM_029887ILMN_2607608
Timm23Translocase of inner mitochondrial membrane 23 homolog (yeast)ENSMUSG00000069622168427.600.0920.0001709344.27E-024.11chr14:32993356:32993405:-3pUTRNM_016897ILMN_2646105
Fis1Fission 1 (mitochondrial outer membrane) homolog (yeast)ENSMUSG000000190546831611.54-0.2210.0001717574.27E-02-4.11chr5:137440995:137441039:+,chr5:137441435:137441439:+CDSNM_025562ILMN_2939295
SpnSialophorinENSMUSG000000514573845727.67-0.0910.0001743314.29E-02-4.10chr7:134279721:134279770:-CDSNM_001037810 NM_009259ILMN_3163543
Col25a1Collagen, type XXV, alpha 1ENSMUSG00000058897770187.65-0.1280.0001795044.33E-02-4.09chr3:130298747:130298796:+3pUTRNM_198711ILMN_2643186
N4bp2l1NEDD4 binding protein 2-like 1ENSMUSG0000004113222876910.11-0.2080.0001796224.33E-02-4.09chr5:151374448:151374497:-3pUTRNM_133898ILMN_1224250
Lrrc48Leucine rich repeat containing 48ENSMUSG00000056598746657.750.1190.0001826674.33E-024.09chr11:60207649:60207698:+CDSNM_029044ILMN_2765593
Adck1AarF domain containing kinase 1ENSMUSG00000021044309489.00-0.2830.0001875624.33E-02-4.08chr12:89699685:89699734:+3pUTRNM_028105ILMN_2656440
Spink10Serine peptidase inhibitor, Kazal type 10ENSMUSG000000441761024427.800.1570.0001885884.33E-024.08chr18:62819573:62819622:+CDSNM_177829ILMN_2694785
Pdik1lPDLIM1 interacting kinase 1 likeENSMUSG000000508902588747.980.1960.000188624.33E-024.08chr4:133831253:133831302:-3pUTRNM_146156ILMN_1220174
Fgd6FYVE, RhoGEF and PH domain containing 6ENSMUSG00000020021662917.780.1110.0001893124.33E-024.08chr10:93604599:93604648:+3pUTRNM_053072ILMN_2894961
NefhNeurofilament, heavy polypeptideENSMUSG00000020396732349.110.3940.000192834.37E-024.07chr11:4839040:4839089:-3pUTRXM_001474024 NM_010904ILMN_1237729
Zfp91Zinc finger protein 91ENSMUSG00000079415695118.180.3220.0001950724.38E-024.07chr19:12844870:12844919:-CDSNM_001039718 NM_053009ILMN_2757889
Pbx1Pre B-cell leukemia transcription factor 12586657.96-0.2970.0002001034.44E-02-4.06chr1:170088153:170088202:-3pUTRXM_001003209 NM_183355ILMN_2587711
Zic3Zinc finger protein of the cerebellum 3ENSMUSG000000678607.880.1520.0002029244.44E-024.05chrX:55289010:55289059:+3pUTRNM_009575ILMN_2440242
Aars2Alanyl-tRNA synthetase 2, mitochondrial (putative)ENSMUSG00000023938115697.860.1150.0002035234.44E-024.05chr17:45657535:45657584:+3pUTRNM_198608ILMN_2876302
Zfp560Zinc finger protein 560ENSMUSG00000045519226348.33-0.2880.0002083544.48E-02-4.04chr9:20189489:20189538:-5pUTRNM_001004190 XM_001476734* XM_001474893*ILMN_1258594
Zfp330Zinc finger protein 330ENSMUSG00000031711309328.88-0.2180.0002102314.48E-02-4.04chr8:85288299:85288348:-CDSNM_145600ILMN_2440789
FasnFatty acid synthaseENSMUSG000000251537.63-0.1040.0002111324.48E-02-4.04chr11:120667910:120667959:-3pUTRNM_007988ILMN_2874104
Ddrgk1DDRGK domain containing 1ENSMUSG0000006829025828611.78-0.2180.0002192064.61E-02-4.03chr2:130479828:130479877:-3pUTRNM_029832ILMN_2615854
Elfn1Leucine rich repeat and fibronectin type III, extracellular 1ENSMUSG00000048988125648.310.1390.0002253944.66E-024.02chr5:140450360:140450409:+3pUTRNM_175522ILMN_2606433
Dtx1Deltex 1 homolog (Drosophila)ENSMUSG000000296038.940.3450.0002256484.66E-024.02chr5:121130444:121130493:-3pUTRNM_008052ILMN_2641793
Slc35e1Solute carrier family 35, member E1ENSMUSG00000019731706509.00-0.2880.0002291514.68E-02-4.01chr8:75005695:75005744:-3pUTRNM_177766ILMN_2752618
Pde8bPhosphodiesterase 8BENSMUSG00000021684200447.69-0.1060.0002323064.68E-02-4.01chr13:95855134:95855183:-CDSILMN_2428320
QarsGlutaminyl-tRNA synthetaseENSMUSG00000032604754248.89-0.2000.0002343354.68E-02-4.01chr9:108416882:108416931:+CDSNM_133794ILMN_2989263
Lrrc4cLeucine rich repeat containing 4CENSMUSG00000050587161827.590.0810.0002345594.68E-024.01chr2:97471041:97471090:+CDSNM_178725ILMN_1247141
1700018C11RikRIKEN cDNA 1700018C11 geneENSMUSG00000058935755247.54-0.0780.0002382984.71E-02-4.00chr4:63268306:63268355:-CDSNM_029324ILMN_2649579
Fam3bFamily with sequence similarity 3, member BENSMUSG000000229383303197.540.0600.0002495914.73E-023.99chr16:97697342:97697352:-,chr16:97696949:97696987:-CDSNM_020622ILMN_2789762
Slc30a3Solute carrier family 30 (zinc transporter), member 3ENSMUSG00000029151227848.12-0.1470.0002538074.73E-02-3.98chr5:31390678:31390689:-,chr5:31390427:31390464:-CDSNM_011773ILMN_2692938
9530068E07RikRIKEN cDNA 9530068E07 geneENSMUSG00000036275679387.60-0.0850.0002542164.73E-02-3.98chr11:52216728:52216777:+CDSNM_153117ILMN_2768859
Eftud2Elongation factor Tu GTP binding domain containing 2ENSMUSG00000020929128049.71-0.2490.0002584284.73E-02-3.98chr11:102700792:102700841:-CDSNM_011431 NM_001109995ILMN_2604097
AI316807Expressed sequence AI316807ENSMUSG000000315345263510.00-0.3180.0002604444.73E-02-3.97chr8:23573108:23573157:-3pUTRNM_001012667ILMN_3160963
Hbp1High mobility group box transcription factor 1ENSMUSG000000029962307848.46-0.3200.000263484.73E-02-3.97chr12:32615773:32615822:-CDSNM_177993 NM_153198ILMN_3092415
Srp19Signal recognition particle 19ENSMUSG000000145042305827.960.1430.0002657914.73E-023.97chr18:34491420:34491469:+CDSNM_025527ILMN_2757224
Prmt7Protein arginine N-methyltransferase 7ENSMUSG000000600982145728.26-0.2420.0002689684.73E-02-3.96chr8:108761180:108761229:+CDSNM_145404ILMN_1252819
Pdcd5Programmed cell death 5ENSMUSG000000304171000424247.820.1410.0002698664.73E-023.96chr7:36428662:36428711:-CDSXM_001478256 NM_019746 XM_001476181ILMN_2756658
Smek2SMEK homolog 2, suppressor of mek1 (Dictyostelium)ENSMUSG00000020463683118.270.3140.000272054.73E-023.96chr11:29119046:29119095:+3pUTRNM_134034ILMN_2907928
Hcn2Hyperpolarization-activated, cyclic nucleotide-gated K+ 2ENSMUSG000000203311314410.360.3890.0002741274.73E-023.96chr10:79198800:79198849:+3pUTRNM_008226ILMN_2672624
Galnt2UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2ENSMUSG000000319771089027.790.1240.0002747564.73E-023.96chr8:126862454:126862469:+,chr8:126864719:126864752:+CDSNM_139272ILMN_1242677
Lpgat1Lysophosphatidylglycerol acyltransferase 1ENSMUSG000000266231707557.630.0820.0002751054.73E-023.96chr1:193602405:193602454:+CDSNM_172266ILMN_2720479
Arpp19CAMP-regulated phosphoprotein 19ENSMUSG00000007656590469.870.2820.0002755094.73E-023.95chr9:74907936:74907985:+3pUTRNM_021548ILMN_2674407
Kcng4Potassium voltage-gated channel, subfamily G, member 4ENSMUSG00000045246669218.020.1520.0002780334.73E-023.95chr8:122148119:122148168:-3pUTRNM_025734ILMN_2895511
7.65-0.1350.0002786284.73E-02-3.95ILMN_2480596
Eef1a1Eukaryotic translation elongation factor 1 alpha 1ENSMUSG000000377421362714.87-0.2860.0002786814.73E-02-3.95chr9:78326765:78326775:-,chr9:78326627:78326665:-CDSNM_010106ILMN_2588052
Carhsp1Calcium regulated heat stable protein 1664119.32-0.1520.0002849034.73E-02-3.94chr16:8658946:8658995:-3pUTRNM_025821ILMN_1225035
Nt5m5,3-nucleotidase, mitochondrialENSMUSG000000326152150088.130.1510.0002857584.73E-023.94chr11:59689624:59689673:+3pUTRNM_134029ILMN_2987854
PhkbPhosphorylase kinase betaENSMUSG000000368791062007.60-0.0840.0002908564.73E-02-3.94chr8:88584180:88584229:+3pUTRNM_199446ILMN_2807757
Ncoa6Nuclear receptor coactivator 6ENSMUSG00000038369219878.61-0.3540.0002952044.73E-02-3.93chr2:155231300:155231349:-CDSNM_019825ILMN_2749937
Cox7bCytochrome c oxidase subunit VIIbENSMUSG00000031231223117.860.2220.0002988244.73E-023.93chrX:103217157:103217206:+CDSNM_025379ILMN_2671689
Lpcat3Lysophosphatidylcholine acyltransferase 3ENSMUSG00000004270664089.29-0.2080.0003000514.73E-02-3.93chr6:124653623:124653645:+,chr6:124653818:124653844:+CDSNM_145130ILMN_2980858
U83174Mus musculus ROSA 26 transcription 1 mRNA sequence.3833487.83-0.1780.0003009544.73E-02-3.93chr6:113027069:113027118:-3pUTRXM_489149* XM_924614*ILMN_1215693
Lhx3LIM homeobox protein 3ENSMUSG00000026934168717.66-0.0960.0003028114.73E-02-3.92chr2:26056071:26056120:-3pUTRNM_001039653ILMN_2784382
Nrp1Neuropilin 1ENSMUSG000000258107424910.780.2880.0003057024.73E-023.92chr8:131027112:131027161:+3pUTRNM_008737ILMN_2669912
NipblNipped-B homolog (Drosophila)ENSMUSG00000022141711759.690.6400.0003122014.73E-023.91chr15:8309179:8309228:-CDSNM_201232 NM_027707ILMN_1234693
Usp28Ubiquitin specific peptidase 28ENSMUSG00000032267699268.27-0.2380.0003123064.73E-02-3.91chr9:48850104:48850153:+3pUTRNM_175482ILMN_2828768
Apoa1bpApolipoprotein A-I binding proteinENSMUSG00000028070146618.680.2850.0003124644.73E-023.91chr3:87860459:87860508:-CDSNM_144897ILMN_1240276
Gm632MKIAA1196 protein (Fragment).ENSMUSG0000000082366620010.75-0.3370.000315024.73E-02-3.91chr2:181316912:181316961:-3pUTRXM_990196 XM_977462ILMN_1219778
EngEndoglinENSMUSG00000026814163337.970.1490.0003171684.73E-023.91chr2:32537324:32537373:+3pUTRNM_007932ILMN_1229161
Dalrd3DALR anticodon binding domain containing 3ENSMUSG000000190392171519.66-0.2860.0003175634.73E-02-3.91chr9:108474567:108474616:+CDSNM_026378ILMN_2644238
2900024O10RikRIKEN cDNA 2900024O10 geneENSMUSG00000051098165789.51-0.2660.0003182184.73E-02-3.91chr13:81891692:81891741:+3pUTRNM_028372ILMN_3160416
GatsOpposite strand transcription unit to Stag3ENSMUSG00000015944182019.540.2930.0003187324.73E-023.91chr5:134617378:134617427:+3pUTRNM_030719ILMN_3036708
Emp2Epithelial membrane protein 2ENSMUSG00000022505137318.39-0.2120.0003225284.73E-02-3.90chr16:10282072:10282121:-3pUTRNM_007929ILMN_2598103
Rpl15Ribosomal protein L15ENSMUSG0000008093232795813.31-0.2350.0003228154.73E-02-3.90chr14:19102467:19102516:-CDSXM_001472467 XM_001472443 XM_001472419 XM_001472396 XR_032689 XM_909622 XM_001480702 NM_025586 XM_001481032 XM_001481031 XM_001473843 XM_001473827 XM_001473807 XM_001473788 XM_001480293ILMN_1243415
SgtbSmall glutamine-rich tetratricopeptide repeat (TPR)-containing, betaENSMUSG000000427431040868.710.3550.0003260264.73E-023.90chr13:104930948:104930997:+3pUTRNM_144838ILMN_1219768
2810441K11Rikhypothetical protein LOC68642ENSMUSG000000246677.610.0610.0003268514.73E-023.90chr19:10625448:10625486:-,chr19:10614298:10614308:-3pUTRNM_026798ILMN_2545299
Commd7COMM domain containing 7ENSMUSG000000569411093498.860.2020.0003311484.73E-023.89chr2:153444612:153444661:-3pUTRNM_133850 XM_905642 XM_979437ILMN_1229682
Ddx5DEAD (Asp-Glu-Ala-Asp) box polypeptide 5ENSMUSG000000824541320712.56-0.2980.0003339784.73E-02-3.89chr11:106643188:106643234:-CDSXR_031177 XR_031980ILMN_1253539
Asphd2Aspartate beta-hydroxylase domain containing 2ENSMUSG000000293487289811.69-0.2630.0003361864.73E-02-3.89chr5:112814553:112814602:-3pUTRNM_028386ILMN_3162247
Mdga2MAM domain containing glycosylphosphatidylinositol anchor 2ENSMUSG000000349128.340.2500.0003382884.73E-023.89chr12:67574189:67574215:-,chr12:67572568:67572590:-CDSNM_207010ILMN_2847711
Ncam1Neural cell adhesion molecule 1730728.04-0.2230.0003388444.73E-02-3.89chr9:49450189:49450238:-3pUTRILMN_1254093
Kcng4Potassium voltage-gated channel, subfamily G, member 4ENSMUSG000000452462356367.860.1140.0003456334.73E-023.88chr8:122147783:122147832:-3pUTRNM_025734ILMN_1233788
mt-Nd4NADH dehydrogenase subunit 4L (EC 1.6.5.3) (Activated spleen cDNA, RIKEN full-length enriched library, clone:F830212L04 product:NADH dehydrogenase 4, mitochondrial, full insert sequence) (3 days neonate thymus cDNA, RIKEN full-length enriched library, clone:A630072D15 product:NADH dehydrogenase 4, mitochondrial, full insert sequence) (Lung RCB-0558 LLC cDNA, RIKEN full-length enriched library, clone:G730042M17 product:NADH dehydrogenase 4, mitochondrial, full insert sequence) (Adult male corpora quadrigemina cDNA, RIKEN full- length enriched library, clone:B230333I23 product:NADH dehydrogenase 4, mitochondrial, full insert sequence).ENSMUSG00000067736125589.220.9760.0003471134.73E-023.88chrM:10117:10166:+CDSILMN_2419660
Thsd7bThrombospondin, type I, domain containing 7BENSMUSG00000042581144508.70-0.2460.0003482354.73E-02-3.88chr1:132115331:132115380:+3pUTRNM_172485ILMN_2949380
AsnsAsparagine synthetaseENSMUSG000000297522312909.920.1830.0003491154.73E-023.88chr6:7625993:7625996:-,chr6:7625479:7625524:-CDSNM_012055ILMN_3006123
Prl2c3Prolactin family 2, subfamily c, member 3ENSMUSG000000564574971067.590.0790.0003498434.73E-023.88chr13:12892287:12892336:-5pUTRNM_011118ILMN_2875585
Fam65bFamily with sequence similarity 65, member BENSMUSG000000360061933859.68-0.1760.0003503274.73E-02-3.88chr13:24825086:24825135:+3pUTRNM_029679ILMN_1255560
Nrxn3Neurexin IIIENSMUSG00000066392987339.560.6640.0003535554.73E-023.87chr12:91571391:91571440:+3pUTRNM_172544ILMN_2763404
Fem1bFeminization 1 homolog b (C. elegans)ENSMUSG000000322447299911.16-0.1680.0003571944.73E-02-3.87chr9:62639952:62640001:-3pUTRNM_010193ILMN_1230162
Cdc7Cell division cycle 7 (S. cerevisiae)ENSMUSG000000292832584279.34-0.2390.0003581184.73E-02-3.87chr5:107413236:107413285:+3pUTRNM_009863ILMN_1238374
Tex11Testis expressed gene 11ENSMUSG000000096702377617.620.0810.0003588814.73E-023.87chrX:98034079:98034128:-3pUTRNM_031384ILMN_2599470
Zfp292Zinc finger protein 292ENSMUSG00000039967669058.330.3750.0003591444.73E-023.87chr4:34752198:34752247:-CDSNM_013889ILMN_1243910
Ctdp1CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1ENSMUSG00000033323936797.540.0610.0003592254.73E-023.87chr18:80605201:80605250:-3pUTRNM_026295ILMN_2650492
CpeCarboxypeptidase EENSMUSG000000378521313514.64-0.3390.0003656694.73E-02-3.86chr8:67073713:67073762:-CDSNM_013494 XM_001002936ILMN_1228232
Thoc7THO complex 7 homolog (Drosophila)ENSMUSG00000053453208227.970.2110.0003656964.73E-023.86chr14:14787053:14787100:-,chr14:14786051:14786052:-CDSNM_001013578 NM_025435 XM_001473442 XM_001473268ILMN_2600729
Chd9Chromodomain helicase DNA binding protein 9ENSMUSG000000566083816287.61-0.0920.000368414.73E-02-3.86chr8:93510011:93510060:+CDSNM_177224ILMN_2600528
S100a10S100 calcium binding protein A10 (calpactin)ENSMUSG00000041959201947.860.2040.0003710814.73E-023.86chr3:93368411:93368460:+3pUTRNM_009112ILMN_1256702
9030025P20RikRIKEN cDNA 9030025P20 geneENSMUSG00000036552180968.42-0.1850.0003750454.73E-02-3.85chr17:15198084:15198133:+CDSNM_001034891 NM_001039552ILMN_2716783
Akap1A kinase (PRKA) anchor protein 1ENSMUSG00000018428135087.580.0660.0003778794.73E-023.85chr11:88725839:88725888:-5pUTRNM_001042541ILMN_3068062
abPartsParts of antibodies, mostly variable regions.ENSMUSG000000765912163457.60-0.0740.0003800784.73E-02-3.85chr6:70337184:70337200:-,chr6:70336949:70336981:-3pUTRILMN_2462709
Rbm12RNA binding motif protein 12ENSMUSG00000038171562198.18-0.1130.0003889284.73E-02-3.84chr2:155921168:155921217:-CDSNM_170598 NM_029397ILMN_3104139
Cacnb3Calcium channel, voltage-dependent, beta 3 subunitENSMUSG000000033523283298.50-0.2330.0003962354.73E-02-3.84chr15:98473935:98473984:+CDSNM_001044741 NM_007581ILMN_2702286
LtkLeukocyte tyrosine kinaseENSMUSG00000027297699228.37-0.1870.0003964674.73E-02-3.84chr2:119577207:119577256:-3pUTRNM_206942 NM_008523 NM_206941 NM_203345ILMN_1219860
Pcdhb14Protocadherin beta 14ENSMUSG00000044043710267.800.1150.000397484.73E-023.83chr18:37609963:37610012:+3pUTRNM_053139ILMN_2607000
Rab23RAB23, member RAS oncogene familyENSMUSG00000004768172078.04-0.1770.0003977834.73E-02-3.83chr1:33798015:33798064:+3pUTRNM_008999ILMN_2687881
Slc5a5Solute carrier family 5 (sodium iodide symporter), member 5ENSMUSG00000000792238219.24-0.1410.0003983044.73E-02-3.83chr8:73406944:73406993:-3pUTRNM_053248ILMN_1245214
Ctnnd2Catenin (cadherin associated protein), delta 2ENSMUSG000000222402329758.92-0.4400.0003991494.73E-02-3.83chr15:30896661:30896710:+CDSXR_032172 NM_008729ILMN_2652527
Rgs3Regulator of G-protein signaling 3ENSMUSG000000598102426467.64-0.0920.000399424.73E-02-3.83chr4:62267576:62267625:+CDSNM_019492ILMN_2610679
Ano9Anoctamin 9ENSMUSG00000054662680357.590.0770.0004026594.73E-023.83chr7:148287144:148287193:-3pUTRNM_178381ILMN_1242413
C330023M02RikRIKEN cDNA C330023M02 geneENSMUSG00000042719227709.390.4070.0004036434.73E-023.83chr5:121887636:121887685:+CDSNM_172722ILMN_1228917
Olfr203Olfactory receptor 203ENSMUSG00000068182200197.570.0780.000404714.73E-023.83chr16:59302997:59303046:+CDSNM_146486ILMN_1214508
Vamp2Vesicle-associated membrane protein 2ENSMUSG000000208946819713.010.1920.0004111844.73E-023.82chr11:68905817:68905866:+3pUTRNM_009497ILMN_2424912
Hist1h2anHistone cluster 1, H2anENSMUSG00000069309142638.390.4410.0004158674.73E-023.82chr13:21878698:21878747:-3pUTRNM_178184ILMN_1248830
Ddx6DEAD (Asp-Glu-Ala-Asp) box polypeptide 6ENSMUSG00000032097664197.850.2200.0004225884.73E-023.81chr9:44443908:44443948:+,chr9:44444572:44444580:+CDSNM_181324 NM_001110826 NM_007841ILMN_3114632
Gpatch2G patch domain containing 2ENSMUSG00000039210677699.10-0.1220.0004228544.73E-02-3.81chr1:189175118:189175167:+3pUTRNM_026367ILMN_2841032
Ptp4a2Protein tyrosine phosphatase 4a2ENSMUSG00000028788676958.79-0.3540.0004278444.73E-02-3.81chr4:129525764:129525813:+3pUTRNM_008974ILMN_1243291
Slitrk4SLIT and NTRK-like family, member 4ENSMUSG00000046699207429.260.5760.0004293984.73E-023.81chrX:61522862:61522911:-3pUTRNM_178740ILMN_2715848
Akap7A kinase (PRKA) anchor protein 7ENSMUSG000000391662584869.76-0.1940.0004325354.73E-02-3.81chr10:24889980:24890029:-3pUTRNM_018747ILMN_2944413
Arfgap2ADP-ribosylation factor GTPase activating protein 2ENSMUSG000000272559.12-0.2310.0004335824.73E-02-3.81chr2:91116851:91116900:+3pUTRNM_023854ILMN_1241481
VegfcVascular endothelial growth factor CENSMUSG00000031520155607.960.1630.0004388654.73E-023.80chr8:55271361:55271410:+CDSNM_009506ILMN_2486573
Elfn2Leucine rich repeat and fibronectin type III, extracellular 2ENSMUSG00000043460508769.440.4760.0004393244.73E-023.80chr15:78500612:78500661:-3pUTRNM_183141ILMN_1239570

Supplementary Table 2. Antibodies used in WB, IP, IHC and IF assays

List of antibodies used in biochemical assays. Antibody: antibody target; Assay: assay in which the antibody was used; Dilution/concentration: Dilution and/or concentration used for each antibody; Company and Catalogue Number: company name and catalogue number for commercially available antibodies.

AntibodyAssayDilution/concentrationCompanyCatalogue Number
b-cateninWB1/2000Cell signaling9582
CRMP2WB-IP1/1000 - 1ug/100ulCell Signaling9393
CRMP2 pT514WB1/1000Cell Signaling9397
DISC1WB-IP1ug/ml- 1ug/100ulInvitrogen40-6800
DISC1WB-IP1ug/ml- 1ug/100ulInvitrogen40-6900
DISC1WB1/500Gift from Nick Brandonn/a
DoublecortinIHC1/250Cell signaling4604
GluR1WB-IP1/5000 - 1ug/100ulMilliporeAB1504
GluR1WB-IP1/2000 - 1ug/100ulMillipore05-855
GlurR1WB1/1000Santa Cruzsc-7609
GSK3-bWB-IP1/1000Cell Signaling9332
Ki67IF1/300Abcamab15580
NeuroD1WB1/1000Cell signaling4373
NeuroD1IF1/500Abcamab60704
NeuroD1 pS274WB1/1000Abcamab78900
NR1WB-IP1/1000 - 1ug/100ulInvitrogen32-0500
NR1IP1ug/100ulCreated in housen/a
NR1WB1ug/mlMillipore06-311
NR2BWB1ug/mlMillipore05-920
NR2BWB1/2000 - 1ug/100ulMillipore06-600
PSD95WB1/10000ThermoMA1-045
PSD95 pT19WB1ug/mlAbcamab16496
PSD95 pS418WB1ug/mlAbcamab16493
Tau pS396WB1/1000Gift from Michel Goedertn/a
TNiKWB-IP0.5ug/ml- 0.25ug/100ulBD biosciences612250
TNiKWB1/10000Santa Cruzsc-100206
TNiKWB1ug/ml- 0.5ug/100ulSigmaHPA012128
TNiKIP1ug/100ulThermoPA1-20639
TNiK pS735WB-IP1ug/ml- 1ug/100ulSanta Cruzsc-130221
TubulinWB1ug/mlMillipore05-559
© G2C 2014. The Genes to Cognition Programme received funding from The Wellcome Trust and the EU FP7 Framework Programmes:
EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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