Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign105 - back to list

Summary
Image
Unique putative probes (0)
Putative probes (369)
Redundant probes (97)

Summary

`./analyse_probe_search_results : Tue Jan 31 11:13:17 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign105
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 13
  id            : 98
  strand        : 1
  -------------

Design window length: 3501bp

All jobs for probe design are successful - total: 56
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 1114 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1114/1114
Putative probes that are unique                 : 0/1114

  No putative probes pass with the above criteria
  Now trying: score-ratio 2, and percent repetitive 40

Putative probes exceeding selection criteria    : 466/1114 (both unique and not unique)
Putative probes with successfully-picked primers: 465/466

Identified 97 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

No probes

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
93971	850	13.9	4250	6.9	1932	0.1
94038	800	13.1	4000	6.6	1960	-0.1
94037	800	13.1	4000	7.0	1920	-0.9
94191	700	11.5	3500	6.4	2065	-1.4
94109	750	12.3	3750	7.1	1924	-1.8
94110	750	12.3	3750	7.1	1961	-1.8
94111	750	12.3	3750	7.1	1998	-1.8
94278	650	10.7	3250	6.9	2080	-3.2
94279	650	10.7	3250	6.9	2112	-3.2
94187	700	11.5	3500	7.6	1925	-3.7
94188	700	11.5	3500	7.6	1960	-3.7
94189	700	11.5	3500	7.6	1995	-3.7
94190	700	11.5	3500	7.6	2030	-3.7
94112	750	12.3	3750	8.3	2035	-4.2
94372	600	9.8	3000	7.5	2070	-5.2
94373	600	9.8	3000	7.5	2100	-5.2
94374	600	9.8	3000	7.5	2130	-5.2
94375	600	9.8	3000	7.5	2160	-5.2
94274	650	10.7	3250	8.2	1952	-5.7
94275	650	10.7	3250	8.2	1984	-5.7
94276	650	10.7	3250	8.2	2016	-5.7
94277	650	10.7	3250	8.2	2048	-5.7
94039	800	13.1	4000	9.6	2000	-6.1
93970	850	13.9	4250	10.1	1890	-6.3
94273	650	10.7	3250	8.6	1920	-6.6
94477	550	9.0	2750	8.2	2079	-7.3
94478	550	9.0	2750	8.2	2106	-7.3
94479	550	9.0	2750	8.2	2133	-7.3
94480	550	9.0	2750	8.2	2160	-7.3
94481	550	9.0	2750	8.2	2187	-7.3
94482	550	9.0	2750	8.2	2214	-7.3
93909	900	14.8	4500	11.1	1890	-7.5
94368	600	9.8	3000	8.8	1950	-7.8
94369	600	9.8	3000	8.8	1980	-7.8
94370	600	9.8	3000	8.8	2010	-7.8
94371	600	9.8	3000	8.8	2040	-7.8
94192	700	11.5	3500	9.9	2100	-8.2
94280	650	10.7	3250	9.7	2144	-8.7
94367	600	9.8	3000	9.3	1920	-8.8
94593	500	8.2	2500	9.0	2075	-9.8
94594	500	8.2	2500	9.0	2100	-9.8
94595	500	8.2	2500	9.0	2125	-9.8
94596	500	8.2	2500	9.0	2150	-9.8
94597	500	8.2	2500	9.0	2175	-9.8
94598	500	8.2	2500	9.0	2200	-9.8
94599	500	8.2	2500	9.0	2225	-9.8
94600	500	8.2	2500	9.0	2250	-9.8
94601	500	8.2	2500	9.0	2275	-9.8
93972	850	13.9	4250	11.9	1974	-9.8
94376	600	9.8	3000	9.8	2190	-9.8
93910	900	14.8	4500	12.4	1935	-10.1
94472	550	9.0	2750	9.6	1944	-10.3
94473	550	9.0	2750	9.6	1971	-10.3
94474	550	9.0	2750	9.6	1998	-10.3
94475	550	9.0	2750	9.6	2025	-10.3
94476	550	9.0	2750	9.6	2052	-10.3
94036	800	13.1	4000	12.0	1880	-10.9
94108	750	12.3	3750	11.9	1887	-11.4
94113	750	12.3	3750	12.1	2072	-12.0
94585	500	16.0	2500	14.2	1875	-12.4
94471	550	9.0	2750	10.7	1917	-12.4
94587	500	8.5	2500	10.6	1925	-12.7
94588	500	8.5	2500	10.6	1950	-12.7
94483	550	9.0	2750	10.9	2241	-12.8
94589	500	8.2	2500	10.6	1975	-13.0
94590	500	8.2	2500	10.6	2000	-13.0
94591	500	8.2	2500	10.6	2025	-13.0
94592	500	8.2	2500	10.6	2050	-13.0
94186	700	11.5	3500	12.3	1890	-13.1
93908	900	14.8	4500	14.6	1845	-14.4
93851	950	15.6	4750	15.8	1833	-16.0
93852	950	15.6	4750	15.8	1880	-16.0
94040	800	13.1	4000	14.6	2040	-16.1
93969	850	13.9	4250	15.1	1848	-16.2
94272	650	10.7	3250	13.5	1888	-16.4
93575	1250	18.3	6250	18.2	1488	-18.2
94193	700	11.5	3500	14.9	2135	-18.2
94281	650	10.7	3250	14.6	2176	-18.6
94366	600	9.8	3000	14.3	1890	-18.8
94586	500	9.5	2500	14.2	1900	-18.9
93540	1300	19.0	6500	19.0	1495	-19.0
94602	500	8.2	2500	13.8	2300	-19.4
94377	600	9.8	3000	14.8	2220	-19.8
94584	500	12.3	2500	16.2	1850	-20.1
93613	1200	17.5	6000	19.0	1500	-20.5
93614	1200	17.5	6000	19.0	1560	-20.5
94035	800	13.1	4000	17.0	1840	-20.9
93853	950	11.2	4750	16.2	1927	-21.2
94107	750	12.3	3750	16.8	1850	-21.3
94470	550	9.4	2750	15.6	1890	-21.9
93576	1250	18.3	6250	20.2	1550	-22.0
94484	550	9.0	2750	15.8	2268	-22.6
93973	850	11.0	4250	16.8	2016	-22.7
93654	1150	16.8	5750	19.8	1539	-22.8
94185	700	11.5	3500	17.3	1855	-23.1
94114	750	10.5	3750	17.1	2109	-23.6
93653	1150	16.8	5750	20.3	1482	-23.7
93798	1000	15.6	5000	20.0	1850	-24.4
93911	900	10.4	4500	17.4	1980	-24.5
93539	1300	19.0	6500	21.9	1430	-24.8
93697	1100	16.1	5500	20.7	1540	-25.4
93698	1100	16.1	5500	20.7	1595	-25.4
93797	1000	14.6	5000	20.0	1800	-25.4
93696	1100	16.1	5500	20.9	1485	-25.7
93907	900	13.2	4500	19.6	1800	-26.0
93968	850	13.9	4250	20.0	1806	-26.1
94271	650	10.7	3250	18.5	1856	-26.3
93656	1150	16.8	5750	22.2	1653	-27.5
93615	1200	17.5	6000	22.7	1620	-27.8
93574	1250	18.3	6250	23.1	1426	-28.0
94041	800	9.2	4000	18.6	2080	-28.0
93742	1050	15.4	5250	21.7	1508	-28.1
93743	1050	15.4	5250	21.7	1560	-28.1
93744	1050	15.4	5250	21.7	1612	-28.1
93745	1050	15.4	5250	21.7	1664	-28.1
93612	1200	17.5	6000	22.9	1440	-28.3
94365	600	10.2	3000	19.3	1860	-28.5
94603	500	8.2	2500	18.8	2325	-29.4
94282	650	9.1	3250	19.5	2208	-30.0
93541	1300	18.0	6500	24.0	1560	-30.0
93799	1000	9.7	5000	20.0	1900	-30.3
94378	600	9.3	3000	19.8	2250	-30.3
93700	1100	16.1	5500	23.4	1705	-30.6
93791	1000	14.6	5000	22.8	1500	-31.0
93792	1000	14.6	5000	22.8	1550	-31.0
93793	1000	14.6	5000	22.8	1600	-31.0
93794	1000	14.6	5000	22.8	1650	-31.0
93961	850	12.4	4250	21.8	1512	-31.1
94194	700	8.6	3500	19.9	2170	-31.1
94106	750	12.3	3750	21.7	1813	-31.2
94469	550	9.5	2750	20.5	1863	-31.6
93747	1050	15.4	5250	23.8	1768	-32.3
94034	800	11.7	4000	22.0	1800	-32.3
94485	550	8.8	2750	20.7	2295	-32.6
94583	500	9.6	2500	21.2	1825	-32.8
94184	700	11.5	3500	22.3	1820	-33.1
94468	550	10.8	2750	22.0	1836	-33.2
93577	1250	17.0	6250	25.1	1612	-33.2
93900	900	13.2	4500	23.3	1485	-33.5
93652	1150	16.8	5750	25.2	1425	-33.6
93741	1050	15.4	5250	24.7	1456	-34.0
94027	800	11.7	4000	22.9	1520	-34.1
94028	800	11.7	4000	22.9	1560	-34.1
93844	950	13.9	4750	24.0	1504	-34.1
93845	950	13.9	4750	24.0	1551	-34.1
93846	950	13.9	4750	24.0	1598	-34.1
93847	950	13.9	4750	24.0	1645	-34.1
93848	950	13.9	4750	24.0	1692	-34.1
94467	550	10.1	2750	22.2	1809	-34.3
93974	850	7.7	4250	21.1	2058	-34.4
93854	950	7.8	4750	21.2	1974	-34.5
93960	850	12.4	4250	23.5	1470	-34.6
93748	1050	12.9	5250	23.8	1820	-34.8
93538	1300	19.0	6500	26.9	1365	-34.8
93695	1100	16.1	5500	25.9	1430	-35.7
94026	800	11.7	4000	23.8	1480	-35.8
93906	900	13.2	4500	24.6	1755	-36.0
94270	650	10.7	3250	23.4	1824	-36.1
94115	750	7.6	3750	22.0	2146	-36.4
93967	850	12.4	4250	24.9	1764	-37.5
93901	900	13.2	4500	25.3	1530	-37.5
93902	900	13.2	4500	25.3	1575	-37.5
93903	900	13.2	4500	25.3	1620	-37.5
93904	900	13.2	4500	25.3	1665	-37.5
93905	900	13.2	4500	25.3	1710	-37.5
93912	900	7.4	4500	22.4	2025	-37.5
94098	750	11.0	3750	24.4	1517	-37.8
94099	750	11.0	3750	24.4	1554	-37.8
94100	750	11.0	3750	24.4	1591	-37.8
93573	1250	18.3	6250	28.1	1364	-37.9
93899	900	13.2	4500	25.6	1440	-38.0
93800	1000	7.3	5000	22.7	1950	-38.1
93611	1200	17.5	6000	27.9	1380	-38.3
93790	1000	14.6	5000	26.5	1450	-38.4
94364	600	10.2	3000	24.3	1830	-38.5
93749	1050	8.8	5250	23.8	1872	-38.8
94097	750	11.0	3750	25.3	1480	-39.7
94604	500	7.8	2500	23.8	2350	-39.8
93843	950	13.9	4750	27.2	1457	-40.4
94042	800	6.7	4000	23.6	2120	-40.5
94400	550	14.9	2750	27.8	0	-40.8
93842	950	13.9	4750	27.4	1410	-40.8
93750	1050	6.8	5250	23.9	1924	-41.0
93962	850	12.4	4250	26.7	1554	-41.0
93963	850	12.4	4250	26.8	1596	-41.2
93964	850	12.4	4250	26.8	1638	-41.2
93965	850	12.4	4250	26.8	1680	-41.2
93966	850	12.4	4250	26.8	1722	-41.2
93616	1200	13.8	6000	27.7	1680	-41.5
94283	650	7.0	3250	24.5	2240	-41.9
94175	700	10.2	3500	26.1	1505	-42.1
94176	700	10.2	3500	26.1	1540	-42.1
94177	700	10.2	3500	26.1	1575	-42.1
94178	700	10.2	3500	26.1	1610	-42.1
94179	700	10.2	3500	26.1	1645	-42.1
94033	800	11.7	4000	27.0	1760	-42.3
94105	750	11.0	3750	26.7	1776	-42.4
94101	750	11.0	3750	26.8	1628	-42.6
94379	600	6.9	3000	24.8	2280	-42.8
94195	700	6.5	3500	24.9	2205	-43.2
93651	1150	16.8	5750	30.2	1368	-43.5
93740	1050	15.4	5250	29.6	1404	-43.9
94029	800	11.7	4000	27.9	1600	-44.1
94183	700	10.2	3500	27.3	1785	-44.3
93959	850	12.4	4250	28.5	1428	-44.5
94486	550	6.7	2750	25.6	2322	-44.5
93537	1300	19.0	6500	31.9	1300	-44.8
93702	1100	9.5	5500	27.3	1815	-45.0
94582	500	7.3	2500	26.2	1800	-45.1
93975	850	6.2	4250	25.8	2100	-45.3
94030	800	11.7	4000	28.5	1640	-45.3
94031	800	11.7	4000	28.5	1680	-45.3
94032	800	11.7	4000	28.5	1720	-45.3
94605	500	6.0	2500	25.8	2375	-45.6
94510	500	13.5	2500	29.6	0	-45.7
94511	500	13.5	2500	29.6	25	-45.7
93694	1100	16.1	5500	30.9	1375	-45.7
94025	800	11.7	4000	28.7	1440	-45.8
93855	950	6.2	4750	26.1	2021	-46.0
94466	550	8.0	2750	27.1	1782	-46.1
94606	500	4.9	2500	25.8	2400	-46.7
94260	650	9.5	3250	28.2	1504	-46.8
94261	650	9.5	3250	28.2	1536	-46.8
94262	650	9.5	3250	28.2	1568	-46.8
94263	650	9.5	3250	28.2	1600	-46.8
94264	650	9.5	3250	28.2	1632	-46.8
94265	650	9.5	3250	28.2	1664	-46.8
94266	650	9.5	3250	28.2	1696	-46.8
94174	700	10.2	3500	28.6	1470	-46.9
94269	650	9.5	3250	28.3	1792	-47.1
93913	900	6.0	4500	26.6	2070	-47.2
93703	1100	7.3	5500	27.3	1870	-47.3
93542	1300	10.6	6500	29.0	1625	-47.4
93789	1000	14.6	5000	31.0	1400	-47.4
94512	500	13.5	2500	30.6	50	-47.7
94180	700	10.2	3500	29.0	1680	-47.8
93898	900	13.2	4500	30.6	1395	-48.0
94116	750	5.8	3750	26.9	2183	-48.1
93704	1100	6.4	5500	27.3	1925	-48.1
94362	600	8.8	3000	28.5	1770	-48.2
94363	600	8.8	3000	28.5	1800	-48.2
93610	1200	17.5	6000	32.9	1320	-48.3
93788	1000	14.6	5000	32.0	1350	-49.4
93801	1000	6.0	5000	27.7	2000	-49.4
94630	500	9.0	2500	29.2	3000	-49.4
94096	750	11.0	3750	30.3	1443	-49.6
94102	750	11.0	3750	30.4	1665	-49.8
94103	750	11.0	3750	30.4	1702	-49.8
94104	750	11.0	3750	30.4	1739	-49.8
94401	550	14.9	2750	32.7	27	-50.6
93841	950	13.9	4750	32.3	1363	-50.7
93751	1050	6.1	5250	28.7	1976	-51.2
94259	650	9.5	3250	30.5	1472	-51.4
94043	800	5.6	4000	28.6	2160	-51.7
94353	600	8.8	3000	30.5	1500	-52.2
94354	600	8.8	3000	30.5	1530	-52.2
94355	600	8.8	3000	30.5	1560	-52.2
94356	600	8.8	3000	30.5	1590	-52.2
94357	600	8.8	3000	30.5	1620	-52.2
94358	600	8.8	3000	30.5	1650	-52.2
94359	600	8.8	3000	30.5	1680	-52.2
94360	600	8.8	3000	30.5	1710	-52.2
94361	600	8.8	3000	30.5	1740	-52.2
94267	650	9.5	3250	30.9	1728	-52.3
93739	1050	15.4	5250	34.3	1352	-53.2
93659	1150	7.5	5750	30.4	1824	-53.4
93650	1150	16.8	5750	35.1	1311	-53.4
93660	1150	6.7	5750	30.3	1881	-53.8
94182	700	10.2	3500	32.3	1750	-54.3
93958	850	12.4	4250	33.4	1386	-54.4
94196	700	5.3	3500	29.9	2240	-54.4
93856	950	5.5	4750	30.0	2068	-54.5
93536	1300	19.0	6500	36.9	1235	-54.8
94181	700	10.2	3500	32.6	1715	-54.9
94581	500	7.3	2500	31.2	1775	-55.1
93738	1050	15.4	5250	35.2	1300	-55.1
93693	1100	16.1	5500	35.9	1320	-55.7
94024	800	11.7	4000	33.8	1400	-55.8
94303	600	11.9	3000	33.8	0	-55.8
94465	550	8.0	2750	32.0	1755	-56.0
93976	850	5.2	4250	30.7	2142	-56.2
93617	1200	9.1	6000	32.7	1740	-56.3
93549	1300	7.6	6500	31.9	2080	-56.3
93550	1300	7.6	6500	31.9	2145	-56.3
94455	550	8.0	2750	32.2	1485	-56.3
93661	1150	6.7	5750	31.6	1938	-56.4
94173	700	10.2	3500	33.6	1435	-56.9
94268	650	9.5	3250	33.2	1760	-57.0
93914	900	5.2	4500	31.3	2115	-57.4
94225	650	5.3	3250	31.4	384	-57.5
93547	1300	7.6	6500	32.5	1950	-57.5
93548	1300	7.6	6500	32.5	2015	-57.5
94513	500	13.5	2500	35.6	75	-57.7
94352	600	8.8	3000	33.3	1470	-57.9
93897	900	13.2	4500	35.6	1350	-58.0
93705	1100	6.4	5500	32.3	1980	-58.1
94456	550	8.0	2750	33.3	1512	-58.5
94457	550	8.0	2750	33.3	1539	-58.5
94458	550	8.0	2750	33.3	1566	-58.5
94459	550	8.0	2750	33.3	1593	-58.5
94460	550	8.0	2750	33.3	1620	-58.5
94461	550	8.0	2750	33.3	1647	-58.5
94462	550	8.0	2750	33.3	1674	-58.5
94463	550	8.0	2750	33.3	1701	-58.5
94464	550	8.0	2750	33.3	1728	-58.5
94117	750	5.0	3750	31.9	2220	-58.8
93587	1250	7.3	6250	33.2	2232	-59.1
93802	1000	5.8	5000	32.5	2050	-59.2
93619	1200	7.0	6000	33.2	1860	-59.3
93620	1200	7.0	6000	33.2	1920	-59.3
94095	750	11.0	3750	35.2	1406	-59.4
93618	1200	7.2	6000	33.3	1800	-59.5
93543	1300	8.0	6500	34.0	1690	-60.0
94224	650	5.3	3250	32.9	352	-60.6
93582	1250	7.3	6250	33.9	1922	-60.6
94488	550	4.5	2750	32.5	2376	-60.6
93761	1000	8.1	5000	34.6	0	-61.1
94489	550	4.0	2750	32.5	2403	-61.1
94316	600	4.9	3000	33.0	390	-61.1
94317	600	4.9	3000	33.0	420	-61.1
93752	1050	6.1	5250	33.6	2028	-61.1
94258	650	9.5	3250	35.4	1440	-61.3
93713	1050	8.5	5250	35.0	0	-61.6
94142	700	5.7	3500	34.0	350	-62.3
94044	800	4.8	4000	33.6	2200	-62.5
94570	500	7.3	2500	35.0	1500	-62.7
94571	500	7.3	2500	35.0	1525	-62.7
94572	500	7.3	2500	35.0	1550	-62.7
93546	1300	7.6	6500	35.5	1885	-63.4
93621	1200	7.0	6000	35.2	1980	-63.5
94315	600	4.9	3000	34.3	360	-63.8
93760	1050	6.1	5250	35.2	2444	-64.4
93857	950	5.5	4750	34.9	2115	-64.4
93580	1250	7.3	6250	35.8	1798	-64.4
93581	1250	7.3	6250	35.8	1860	-64.4
94402	550	10.4	2750	37.6	54	-64.9
94580	500	7.3	2500	36.2	1750	-65.1
93812	950	7.7	4750	36.4	0	-65.1
93813	950	7.7	4750	36.4	47	-65.1
93762	1000	8.1	5000	36.8	50	-65.5
93668	1150	6.7	5750	36.1	2337	-65.5
93977	850	4.9	4250	35.4	2184	-65.9
94573	500	7.3	2500	36.6	1575	-65.9
94574	500	7.3	2500	36.6	1600	-65.9
94575	500	7.3	2500	36.6	1625	-65.9
94576	500	7.3	2500	36.6	1650	-65.9
94577	500	7.3	2500	36.6	1675	-65.9
94578	500	7.3	2500	36.6	1700	-65.9
94579	500	7.3	2500	36.6	1725	-65.9
94141	700	5.7	3500	35.9	315	-66.0
93669	1100	8.9	5500	38.0	0	-67.1
94143	700	5.7	3500	36.4	385	-67.2
94226	650	5.3	3250	36.3	416	-67.3
93915	900	5.2	4500	36.3	2160	-67.4
94414	550	4.5	2750	36.0	378	-67.5
94415	550	4.5	2750	36.0	405	-67.5
94416	550	4.5	2750	36.0	432	-67.5
94417	550	4.5	2750	36.0	459	-67.5
93803	1000	5.8	5000	36.7	2100	-67.6
93811	1000	5.8	5000	37.0	2500	-68.2
94318	600	4.9	3000	36.7	450	-68.5
93544	1300	7.6	6500	38.3	1755	-69.0
93545	1300	7.6	6500	38.3	1820	-69.0
94118	750	4.4	3750	36.8	2257	-69.2
94223	650	5.3	3250	37.8	320	-70.4
94418	550	4.5	2750	37.5	486	-70.4
94067	750	6.1	3750	38.7	370	-71.2
93714	1050	8.5	5250	40.0	52	-71.5
94383	600	3.6	3000	38.2	2400	-72.7

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
93655	1150	16.8	5750	19.8	1596	-22.8
93699	1100	16.1	5500	20.7	1650	-25.4
93850	950	13.9	4750	20.0	1786	-26.1
93746	1050	15.4	5250	21.7	1716	-28.1
93796	1000	14.6	5000	22.6	1750	-30.6
93795	1000	14.6	5000	22.8	1700	-31.0
93849	950	13.9	4750	24.0	1739	-34.1
93657	1150	15.9	5750	27.1	1710	-38.3
93701	1100	15.2	5500	27.3	1760	-39.3
93572	1250	18.3	6250	33.0	1302	-47.8
93578	1250	10.3	6250	30.1	1674	-49.9
93658	1150	9.9	5750	30.4	1767	-51.0
94284	650	5.4	3250	29.4	2272	-53.3
94380	600	5.3	3000	29.8	2310	-54.3
94607	500	4.1	2500	29.8	2425	-55.5
94487	550	5.4	2750	30.5	2349	-55.7
93571	1250	18.3	6250	38.0	1240	-57.7
93609	1200	17.5	6000	37.9	1260	-58.3
93692	1100	16.1	5500	37.3	1265	-58.5
93585	1250	7.3	6250	33.2	2108	-59.1
93586	1250	7.3	6250	33.2	2170	-59.1
93787	1000	14.6	5000	37.0	1300	-59.4
94629	500	9.0	2500	34.2	2975	-59.4
93583	1250	7.3	6250	33.8	1984	-60.4
93584	1250	7.3	6250	33.8	2046	-60.4
93840	950	13.9	4750	37.3	1316	-60.6
93623	1200	7.0	6000	34.6	2100	-62.2
93624	1200	7.0	6000	34.6	2160	-62.2
93625	1200	7.0	6000	34.6	2220	-62.2
93626	1200	7.0	6000	34.6	2280	-62.2
93579	1250	7.7	6250	35.0	1736	-62.4
93649	1150	16.8	5750	40.0	1254	-63.2
93622	1200	7.0	6000	35.2	2040	-63.5
94285	650	4.7	3250	34.3	2304	-63.9
94509	550	9.9	2750	36.9	2943	-63.9
93957	850	12.4	4250	38.4	1344	-64.3
94381	600	4.5	3000	34.8	2340	-65.1
94197	700	4.5	3500	34.9	2275	-65.2
94351	600	8.8	3000	37.0	1440	-65.2
93664	1150	6.7	5750	36.1	2109	-65.5
93665	1150	6.7	5750	36.1	2166	-65.5
93666	1150	6.7	5750	36.1	2223	-65.5
93667	1150	6.7	5750	36.1	2280	-65.5
94023	800	11.7	4000	38.8	1360	-65.8
94608	500	3.7	2500	34.8	2450	-65.9
94454	550	8.0	2750	37.1	1458	-66.1
93662	1150	6.7	5750	36.5	1995	-66.3
93759	1050	6.1	5250	36.5	2392	-66.8
93663	1150	6.7	5750	36.8	2052	-66.9
94172	700	10.2	3500	38.6	1400	-66.9
94569	500	7.3	2500	37.4	1475	-67.5
93706	1100	6.4	5500	37.3	2035	-68.1
93712	1100	6.4	5500	37.3	2365	-68.1
93810	1000	5.8	5000	37.0	2450	-68.2
93814	950	7.7	4750	38.1	94	-68.5
93707	1100	6.4	5500	37.7	2090	-69.1
93708	1100	6.4	5500	37.7	2145	-69.1
93709	1100	6.4	5500	37.7	2200	-69.1
93710	1100	6.4	5500	37.7	2255	-69.1
93711	1100	6.4	5500	37.7	2310	-69.1
93809	1000	5.8	5000	37.5	2400	-69.2
94304	600	8.5	3000	38.8	30	-69.2
94628	500	9.0	2500	39.2	2950	-69.4
93753	1050	6.1	5250	37.8	2080	-69.5
93867	900	7.3	4500	38.4	0	-69.6
93868	900	7.3	4500	38.4	45	-69.6
93869	900	7.3	4500	38.4	90	-69.6
94213	650	7.9	3250	38.9	0	-69.9
94257	650	9.5	3250	40.0	1408	-70.5
94490	550	3.5	2750	37.1	2430	-70.7
94065	750	6.1	3750	38.4	296	-70.7
94066	750	6.1	3750	38.4	333	-70.7
93870	900	7.3	4500	39.2	135	-71.1
94045	800	4.7	4000	38.4	2240	-72.1
93864	950	5.5	4750	38.9	2444	-72.4
93865	950	5.5	4750	38.9	2491	-72.4
93866	950	5.5	4750	38.9	2538	-72.4
94382	600	4.0	3000	38.2	2370	-72.4
93754	1050	6.1	5250	39.5	2132	-72.9
93755	1050	6.1	5250	39.5	2184	-72.9
93756	1050	6.1	5250	39.5	2236	-72.9
93757	1050	6.1	5250	39.5	2288	-72.9
93758	1050	6.1	5250	39.5	2340	-72.9
94413	550	4.5	2750	39.1	351	-73.7
94314	600	4.9	3000	39.3	330	-73.8
93863	950	5.5	4750	39.8	2397	-74.0
93858	950	5.5	4750	39.9	2162	-74.3
94286	650	4.0	3250	39.2	2336	-74.4
94525	500	4.1	2500	39.6	375	-75.1
94526	500	4.1	2500	39.6	400	-75.1
94527	500	4.1	2500	39.6	425	-75.1
94528	500	4.1	2500	39.6	450	-75.1
94529	500	4.1	2500	39.6	475	-75.1
94530	500	4.1	2500	39.6	500	-75.1
94531	500	4.1	2500	39.6	525	-75.1
94198	700	4.1	3500	39.9	2310	-75.6
94609	500	3.2	2500	39.8	2475	-76.4

Overall score = score_ratio - 2 * repetitive DNA content