Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign106 - back to list

Summary
Image
Unique putative probes (65)
Putative probes (525)
Redundant probes (351)

Summary

`./analyse_probe_search_results : Tue Jan 31 11:17:15 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign106
  assembly      : NCBIM34
  bias          : 5prime
  chromosome    : 4
  id            : 89
  strand        : 1
  -------------

Design window length: 3087bp

All jobs for probe design are successful - total: 48
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 941 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 941/941
Putative probes that are unique                 : 65/941
Putative probes exceeding selection criteria    : 941/941 (both unique and not unique)
Putative probes with successfully-picked primers: 772/941

Identified 351 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
82785	750	3750	-	1480
82921	650	3250	-	1376
82922	650	3250	-	1408
83001	600	3000	-	1380
83002	600	3000	-	1410
83003	600	3000	-	1440
83089	550	2750	-	1377
83090	550	2750	-	1404
83091	550	2750	-	1431
83092	550	2750	-	1458
83093	550	2750	-	1485
83122	550	2750	-	2268
83143	500	2500	-	275
83144	500	2500	-	300
83169	500	2500	-	925
83187	500	2500	-	1375
83188	500	2500	-	1400
83189	500	2500	-	1425
83190	500	2500	-	1450
83191	500	2500	-	1475
83192	500	2500	-	1500
83193	500	2500	-	1525
83194	500	2500	-	1550
83223	500	2500	-	2275
83224	500	2500	-	2300
83225	500	2500	-	2325
82744	800	4000	1.6	2280
82807	750	3750	1.7	2294
82808	750	3750	1.7	2331
82874	700	3500	1.9	2275
82875	700	3500	1.9	2310
82876	700	3500	1.9	2345
82877	700	3500	1.9	2380
82949	650	3250	2.0	2272
82950	650	3250	2.0	2304
82951	650	3250	2.0	2336
82952	650	3250	2.0	2368
82953	650	3250	2.0	2400
82954	650	3250	2.0	2432
83123	550	2750	2.0	2295
83031	600	3000	2.2	2280
83032	600	3000	2.2	2310
83033	600	3000	2.2	2340
83034	600	3000	2.2	2370
83035	600	3000	2.2	2400
83036	600	3000	2.2	2430
83037	600	3000	2.2	2460
83124	550	2750	2.4	2322
83125	550	2750	2.4	2349
83126	550	2750	2.4	2376
83127	550	2750	2.4	2403
83128	550	2750	2.4	2430
83129	550	2750	2.4	2457
83130	550	2750	2.4	2484
83131	550	2750	2.4	2511
83226	500	2500	2.6	2350
83227	500	2500	2.6	2375
83228	500	2500	2.6	2400
83229	500	2500	2.6	2425
83230	500	2500	2.6	2450
83231	500	2500	2.6	2475
83232	500	2500	2.6	2500
83233	500	2500	2.6	2525
83234	500	2500	2.6	2550
83235	500	2500	2.6	2575

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
82309	1300	41.9	6500	-	910	41.9
82338	1250	40.3	6250	-	930	40.3
82369	1200	38.7	6000	-	960	38.7
82370	1200	38.7	6000	-	1020	38.7
82368	1200	38.5	6000	0.1	900	38.3
82307	1300	41.4	6500	1.7	780	38.0
82308	1300	41.4	6500	1.7	845	38.0
82401	1150	37.1	5750	-	912	37.1
82402	1150	37.1	5750	-	969	37.1
82403	1150	37.1	5750	-	1026	37.1
82436	1100	35.5	5500	-	935	35.5
82437	1100	35.5	5500	-	990	35.5
82438	1100	35.5	5500	-	1045	35.5
82439	1100	35.5	5500	-	1100	35.5
82474	1050	33.9	5250	-	936	33.9
82475	1050	33.9	5250	-	988	33.9
82476	1050	33.9	5250	-	1040	33.9
82477	1050	33.9	5250	-	1092	33.9
82478	1050	33.9	5250	-	1144	33.9
82479	1050	33.0	5250	-	1196	33.0
82310	1300	32.5	6500	-	975	32.5
82311	1300	32.5	6500	-	1040	32.5
82312	1300	32.5	6500	-	1105	32.5
82313	1300	32.5	6500	-	1170	32.5
82314	1300	32.5	6500	-	1235	32.5
82315	1300	32.5	6500	-	1300	32.5
82316	1300	32.5	6500	-	1365	32.5
82317	1300	32.5	6500	-	1430	32.5
82318	1300	32.5	6500	-	1495	32.5
82515	1000	32.3	5000	-	950	32.3
82516	1000	32.3	5000	-	1000	32.3
82517	1000	32.3	5000	-	1050	32.3
82518	1000	32.3	5000	-	1100	32.3
82519	1000	32.3	5000	-	1150	32.3
82520	1000	32.3	5000	-	1200	32.3
82514	1000	32.1	5000	0.1	900	31.9
82521	1000	31.4	5000	-	1250	31.4
82339	1250	31.2	6250	-	992	31.2
82340	1250	31.2	6250	-	1054	31.2
82341	1250	31.2	6250	-	1116	31.2
82342	1250	31.2	6250	-	1178	31.2
82343	1250	31.2	6250	-	1240	31.2
82344	1250	31.2	6250	-	1302	31.2
82345	1250	31.2	6250	-	1364	31.2
82346	1250	31.2	6250	-	1426	31.2
82347	1250	31.2	6250	-	1488	31.2
82348	1250	31.2	6250	-	1550	31.2
82558	950	30.6	4750	-	940	30.6
82559	950	30.6	4750	-	987	30.6
82560	950	30.6	4750	-	1034	30.6
82561	950	30.6	4750	-	1081	30.6
82562	950	30.6	4750	-	1128	30.6
82563	950	30.6	4750	-	1175	30.6
82564	950	30.6	4750	-	1222	30.6
82565	950	30.6	4750	-	1269	30.6
82319	1300	32.5	6500	1.0	1560	30.5
82320	1300	32.5	6500	1.0	1625	30.5
82321	1300	32.5	6500	1.0	1690	30.5
82322	1300	32.5	6500	1.0	1755	30.5
82473	1050	33.4	5250	1.6	884	30.2
82371	1200	30.0	6000	-	1080	30.0
82372	1200	30.0	6000	-	1140	30.0
82373	1200	30.0	6000	-	1200	30.0
82374	1200	30.0	6000	-	1260	30.0
82375	1200	30.0	6000	-	1320	30.0
82376	1200	30.0	6000	-	1380	30.0
82377	1200	30.0	6000	-	1440	30.0
82378	1200	30.0	6000	-	1500	30.0
82379	1200	30.0	6000	-	1560	30.0
82380	1200	30.0	6000	-	1620	30.0
82566	950	29.5	4750	-	1316	29.5
82349	1250	31.2	6250	1.0	1612	29.2
82605	900	29.0	4500	-	945	29.0
82606	900	29.0	4500	-	990	29.0
82607	900	29.0	4500	-	1035	29.0
82608	900	29.0	4500	-	1080	29.0
82609	900	29.0	4500	-	1125	29.0
82610	900	29.0	4500	-	1170	29.0
82611	900	29.0	4500	-	1215	29.0
82612	900	29.0	4500	-	1260	29.0
82613	900	29.0	4500	-	1305	29.0
82557	950	30.4	4750	0.8	893	28.8
82404	1150	28.8	5750	-	1083	28.8
82405	1150	28.8	5750	-	1140	28.8
82406	1150	28.8	5750	-	1197	28.8
82407	1150	28.8	5750	-	1254	28.8
82408	1150	28.8	5750	-	1311	28.8
82409	1150	28.8	5750	-	1368	28.8
82410	1150	28.8	5750	-	1425	28.8
82411	1150	28.8	5750	-	1482	28.8
82412	1150	28.8	5750	-	1539	28.8
82413	1150	28.8	5750	-	1596	28.8
82414	1150	28.8	5750	-	1653	28.8
82604	900	28.8	4500	0.1	900	28.6
82614	900	28.3	4500	-	1350	28.3
82384	1200	30.0	6000	1.1	1860	27.8
82440	1100	27.5	5500	-	1155	27.5
82441	1100	27.5	5500	-	1210	27.5
82442	1100	27.5	5500	-	1265	27.5
82443	1100	27.5	5500	-	1320	27.5
82444	1100	27.5	5500	-	1375	27.5
82445	1100	27.5	5500	-	1430	27.5
82446	1100	27.5	5500	-	1485	27.5
82447	1100	27.5	5500	-	1540	27.5
82448	1100	27.5	5500	-	1595	27.5
82449	1100	27.5	5500	-	1650	27.5
82450	1100	27.5	5500	-	1705	27.5
82295	1300	30.8	6500	1.7	0	27.4
82296	1300	30.8	6500	1.7	65	27.4
82297	1300	30.8	6500	1.7	130	27.4
82298	1300	30.8	6500	1.7	195	27.4
82299	1300	30.8	6500	1.7	260	27.4
82300	1300	30.8	6500	1.7	325	27.4
82301	1300	30.8	6500	1.7	390	27.4
82302	1300	30.8	6500	1.7	455	27.4
82303	1300	30.8	6500	1.7	520	27.4
82304	1300	30.8	6500	1.7	585	27.4
82305	1300	30.8	6500	1.7	650	27.4
82306	1300	30.8	6500	1.7	715	27.4
82655	850	27.4	4250	-	924	27.4
82656	850	27.4	4250	-	966	27.4
82657	850	27.4	4250	-	1008	27.4
82658	850	27.4	4250	-	1050	27.4
82659	850	27.4	4250	-	1092	27.4
82660	850	27.4	4250	-	1134	27.4
82661	850	27.4	4250	-	1176	27.4
82662	850	27.4	4250	-	1218	27.4
82663	850	27.4	4250	-	1260	27.4
82664	850	27.4	4250	-	1302	27.4
82665	850	27.4	4250	-	1344	27.4
82666	850	27.2	4250	-	1386	27.2
82667	850	26.4	4250	-	1428	26.4
82480	1050	26.2	5250	-	1248	26.2
82481	1050	26.2	5250	-	1300	26.2
82482	1050	26.2	5250	-	1352	26.2
82483	1050	26.2	5250	-	1404	26.2
82484	1050	26.2	5250	-	1456	26.2
82485	1050	26.2	5250	-	1508	26.2
82486	1050	26.2	5250	-	1560	26.2
82487	1050	26.2	5250	-	1612	26.2
82488	1050	26.2	5250	-	1664	26.2
82489	1050	26.2	5250	-	1716	26.2
82490	1050	26.2	5250	-	1768	26.2
82324	1250	29.6	6250	1.8	62	26.1
82325	1250	29.6	6250	1.8	124	26.1
82326	1250	29.6	6250	1.8	186	26.1
82327	1250	29.6	6250	1.8	248	26.1
82721	800	26.0	4000	-	1360	26.0
82710	800	25.8	4000	-	920	25.8
82711	800	25.8	4000	-	960	25.8
82712	800	25.8	4000	-	1000	25.8
82713	800	25.8	4000	-	1040	25.8
82714	800	25.8	4000	-	1080	25.8
82715	800	25.8	4000	-	1120	25.8
82716	800	25.8	4000	-	1160	25.8
82717	800	25.8	4000	-	1200	25.8
82718	800	25.8	4000	-	1240	25.8
82719	800	25.8	4000	-	1280	25.8
82720	800	25.8	4000	-	1320	25.8
82722	800	25.8	4000	-	1400	25.8
82687	800	25.5	4000	-	0	25.5
82723	800	25.3	4000	-	1440	25.3
82455	1100	27.5	5500	1.2	1980	25.1
82522	1000	25.0	5000	-	1300	25.0
82523	1000	25.0	5000	-	1350	25.0
82524	1000	25.0	5000	-	1400	25.0
82525	1000	25.0	5000	-	1450	25.0
82526	1000	25.0	5000	-	1500	25.0
82527	1000	25.0	5000	-	1550	25.0
82528	1000	25.0	5000	-	1600	25.0
82529	1000	25.0	5000	-	1650	25.0
82530	1000	25.0	5000	-	1700	25.0
82531	1000	25.0	5000	-	1750	25.0
82532	1000	25.0	5000	-	1800	25.0
82782	750	24.8	3750	-	1369	24.8
82783	750	24.8	3750	-	1406	24.8
82770	750	24.2	3750	-	925	24.2
82771	750	24.2	3750	-	962	24.2
82772	750	24.2	3750	-	999	24.2
82773	750	24.2	3750	-	1036	24.2
82774	750	24.2	3750	-	1073	24.2
82775	750	24.2	3750	-	1110	24.2
82776	750	24.2	3750	-	1147	24.2
82777	750	24.2	3750	-	1184	24.2
82778	750	24.2	3750	-	1221	24.2
82779	750	24.2	3750	-	1258	24.2
82780	750	24.2	3750	-	1295	24.2
82781	750	24.2	3750	-	1332	24.2
82784	750	24.2	3750	-	1443	24.2
82745	750	23.9	3750	-	0	23.9
82746	750	23.9	3750	-	37	23.9
82567	950	23.8	4750	-	1363	23.8
82568	950	23.8	4750	-	1410	23.8
82569	950	23.8	4750	-	1457	23.8
82570	950	23.8	4750	-	1504	23.8
82571	950	23.8	4750	-	1551	23.8
82572	950	23.8	4750	-	1598	23.8
82573	950	23.8	4750	-	1645	23.8
82574	950	23.8	4750	-	1692	23.8
82575	950	23.8	4750	-	1739	23.8
82576	950	23.8	4750	-	1786	23.8
82577	950	23.8	4750	-	1833	23.8
82578	950	23.8	4750	-	1880	23.8
82786	750	23.3	3750	-	1517	23.3
82848	700	23.2	3500	-	1365	23.2
82849	700	23.2	3500	-	1400	23.2
82850	700	23.2	3500	-	1435	23.2
82811	700	23.0	3500	-	70	23.0
82835	700	22.6	3500	-	910	22.6
82836	700	22.6	3500	-	945	22.6
82837	700	22.6	3500	-	980	22.6
82838	700	22.6	3500	-	1015	22.6
82839	700	22.6	3500	-	1050	22.6
82840	700	22.6	3500	-	1085	22.6
82841	700	22.6	3500	-	1120	22.6
82842	700	22.6	3500	-	1155	22.6
82843	700	22.6	3500	-	1190	22.6
82844	700	22.6	3500	-	1225	22.6
82845	700	22.6	3500	-	1260	22.6
82846	700	22.6	3500	-	1295	22.6
82847	700	22.6	3500	-	1330	22.6
82851	700	22.6	3500	-	1470	22.6
82852	700	22.6	3500	-	1505	22.6
82615	900	22.5	4500	-	1395	22.5
82616	900	22.5	4500	-	1440	22.5
82617	900	22.5	4500	-	1485	22.5
82618	900	22.5	4500	-	1530	22.5
82619	900	22.5	4500	-	1575	22.5
82620	900	22.5	4500	-	1620	22.5
82621	900	22.5	4500	-	1665	22.5
82622	900	22.5	4500	-	1710	22.5
82623	900	22.5	4500	-	1755	22.5
82624	900	22.5	4500	-	1800	22.5
82625	900	22.5	4500	-	1845	22.5
82626	900	22.5	4500	-	1890	22.5
82809	700	22.3	3500	-	0	22.3
82810	700	22.3	3500	-	35	22.3
82627	900	22.5	4500	0.1	1935	22.3
82747	750	22.2	3750	-	74	22.2
82812	700	22.2	3500	-	105	22.2
82853	700	22.2	3500	-	1540	22.2
82854	700	21.7	3500	-	1575	21.7
82881	650	21.7	3250	-	96	21.7
82882	650	21.7	3250	-	128	21.7
82688	800	21.6	4000	-	40	21.6
82923	650	21.5	3250	-	1440	21.5
82924	650	21.5	3250	-	1472	21.5
82925	650	21.5	3250	-	1504	21.5
82668	850	21.2	4250	-	1470	21.2
82669	850	21.2	4250	-	1512	21.2
82670	850	21.2	4250	-	1554	21.2
82671	850	21.2	4250	-	1596	21.2
82672	850	21.2	4250	-	1638	21.2
82673	850	21.2	4250	-	1680	21.2
82674	850	21.2	4250	-	1722	21.2
82675	850	21.2	4250	-	1764	21.2
82676	850	21.2	4250	-	1806	21.2
82677	850	21.2	4250	-	1848	21.2
82678	850	21.2	4250	-	1890	21.2
82679	850	21.2	4250	-	1932	21.2
82680	850	21.2	4250	-	1974	21.2
82920	650	21.1	3250	-	1344	21.1
82907	650	21.0	3250	-	928	21.0
82908	650	21.0	3250	-	960	21.0
82909	650	21.0	3250	-	992	21.0
82910	650	21.0	3250	-	1024	21.0
82911	650	21.0	3250	-	1056	21.0
82912	650	21.0	3250	-	1088	21.0
82913	650	21.0	3250	-	1120	21.0
82914	650	21.0	3250	-	1152	21.0
82915	650	21.0	3250	-	1184	21.0
82916	650	21.0	3250	-	1216	21.0
82917	650	21.0	3250	-	1248	21.0
82918	650	21.0	3250	-	1280	21.0
82919	650	21.0	3250	-	1312	21.0
82926	650	21.0	3250	-	1536	21.0
82927	650	21.0	3250	-	1568	21.0
82878	650	20.7	3250	-	0	20.7
82879	650	20.7	3250	-	32	20.7
82880	650	20.7	3250	-	64	20.7
82928	650	20.4	3250	-	1600	20.4
82883	650	20.3	3250	-	160	20.3
82633	850	20.1	4250	-	0	20.1
82958	600	20.0	3000	-	90	20.0
82959	600	20.0	3000	-	120	20.0
82960	600	20.0	3000	-	150	20.0
82961	600	20.0	3000	-	180	20.0
82724	800	20.0	4000	-	1480	20.0
82725	800	20.0	4000	-	1520	20.0
82726	800	20.0	4000	-	1560	20.0
82727	800	20.0	4000	-	1600	20.0
82728	800	20.0	4000	-	1640	20.0
82729	800	20.0	4000	-	1680	20.0
82730	800	20.0	4000	-	1720	20.0
82731	800	20.0	4000	-	1760	20.0
82732	800	20.0	4000	-	1800	20.0
82733	800	20.0	4000	-	1840	20.0
82734	800	20.0	4000	-	1880	20.0
82735	800	20.0	4000	-	1920	20.0
82736	800	20.0	4000	-	1960	20.0
82737	800	20.0	4000	-	2000	20.0
83004	600	19.9	3000	-	1470	19.9
83005	600	19.9	3000	-	1500	19.9
83006	600	19.9	3000	-	1530	19.9
83000	600	19.5	3000	-	1350	19.5
83007	600	19.5	3000	-	1560	19.5
82986	600	19.4	3000	-	930	19.4
82987	600	19.4	3000	-	960	19.4
82988	600	19.4	3000	-	990	19.4
82989	600	19.4	3000	-	1020	19.4
82990	600	19.4	3000	-	1050	19.4
82991	600	19.4	3000	-	1080	19.4
82992	600	19.4	3000	-	1110	19.4
82993	600	19.4	3000	-	1140	19.4
82994	600	19.4	3000	-	1170	19.4
82995	600	19.4	3000	-	1200	19.4
82996	600	19.4	3000	-	1230	19.4
82997	600	19.4	3000	-	1260	19.4
82998	600	19.4	3000	-	1290	19.4
82999	600	19.4	3000	-	1320	19.4
83008	600	19.4	3000	-	1590	19.4
83009	600	19.4	3000	-	1620	19.4
82906	650	20.8	3250	0.8	896	19.3
82955	600	19.1	3000	-	0	19.1
82956	600	19.1	3000	-	30	19.1
82957	600	19.1	3000	-	60	19.1
82869	700	19.0	3500	-	2100	19.0
82813	700	18.9	3500	-	140	18.9
82985	600	19.2	3000	0.2	900	18.9
83010	600	18.9	3000	-	1650	18.9
82962	600	18.8	3000	-	210	18.8
82787	750	18.8	3750	-	1554	18.8
82788	750	18.8	3750	-	1591	18.8
82789	750	18.8	3750	-	1628	18.8
82790	750	18.8	3750	-	1665	18.8
82791	750	18.8	3750	-	1702	18.8
82792	750	18.8	3750	-	1739	18.8
82793	750	18.8	3750	-	1776	18.8
82794	750	18.8	3750	-	1813	18.8
82795	750	18.8	3750	-	1850	18.8
82796	750	18.8	3750	-	1887	18.8
82797	750	18.8	3750	-	1924	18.8
82798	750	18.8	3750	-	1961	18.8
82799	750	18.8	3750	-	1998	18.8
82800	750	18.8	3750	-	2035	18.8
82801	750	18.8	3750	-	2072	18.8
82870	700	19.0	3500	0.1	2135	18.7
82689	800	19.0	4000	0.1	80	18.7
82738	800	20.0	4000	0.8	2040	18.5
83041	550	18.3	2750	-	81	18.3
83042	550	18.3	2750	-	108	18.3
83043	550	18.3	2750	-	135	18.3
83044	550	18.3	2750	-	162	18.3
83045	550	18.3	2750	-	189	18.3
83046	550	18.3	2750	-	216	18.3
83047	550	18.3	2750	-	243	18.3
83094	550	18.2	2750	-	1512	18.2
83095	550	18.2	2750	-	1539	18.2
83096	550	18.2	2750	-	1566	18.2
83097	550	18.2	2750	-	1593	18.2
83088	550	17.9	2750	-	1350	17.9
82748	750	17.8	3750	-	111	17.8
83072	550	17.7	2750	-	918	17.7
83073	550	17.7	2750	-	945	17.7
83074	550	17.7	2750	-	972	17.7
83075	550	17.7	2750	-	999	17.7
83076	550	17.7	2750	-	1026	17.7
83077	550	17.7	2750	-	1053	17.7
83078	550	17.7	2750	-	1080	17.7
83079	550	17.7	2750	-	1107	17.7
83080	550	17.7	2750	-	1134	17.7
83081	550	17.7	2750	-	1161	17.7
83082	550	17.7	2750	-	1188	17.7
83083	550	17.7	2750	-	1215	17.7
83084	550	17.7	2750	-	1242	17.7
83085	550	17.7	2750	-	1269	17.7
83086	550	17.7	2750	-	1296	17.7
83087	550	17.7	2750	-	1323	17.7
83098	550	17.7	2750	-	1620	17.7
83099	550	17.7	2750	-	1647	17.7
83100	550	17.7	2750	-	1674	17.7
82944	650	17.7	3250	-	2112	17.7
82945	650	17.7	3250	-	2144	17.7
82946	650	17.7	3250	-	2176	17.7
83038	550	17.5	2750	-	0	17.5
83039	550	17.5	2750	-	27	17.5
83040	550	17.5	2750	-	54	17.5
82855	700	17.5	3500	-	1610	17.5
82856	700	17.5	3500	-	1645	17.5
82857	700	17.5	3500	-	1680	17.5
82858	700	17.5	3500	-	1715	17.5
82859	700	17.5	3500	-	1750	17.5
82860	700	17.5	3500	-	1785	17.5
82861	700	17.5	3500	-	1820	17.5
82862	700	17.5	3500	-	1855	17.5
82863	700	17.5	3500	-	1890	17.5
82864	700	17.5	3500	-	1925	17.5
82865	700	17.5	3500	-	1960	17.5
82866	700	17.5	3500	-	1995	17.5
82867	700	17.5	3500	-	2030	17.5
82868	700	17.5	3500	-	2065	17.5
83028	600	17.4	3000	-	2190	17.4
83029	600	17.4	3000	-	2220	17.4
83101	550	17.3	2750	-	1701	17.3
82634	850	20.1	4250	1.5	42	17.1
83102	550	17.1	2750	-	1728	17.1
83135	500	16.7	2500	-	75	16.7
83136	500	16.7	2500	-	100	16.7
83137	500	16.7	2500	-	125	16.7
83138	500	16.7	2500	-	150	16.7
83139	500	16.7	2500	-	175	16.7
82884	650	16.7	3250	-	192	16.7
83140	500	16.7	2500	-	200	16.7
83141	500	16.7	2500	-	225	16.7
83142	500	16.7	2500	-	250	16.7
82814	700	16.6	3500	-	175	16.6
83195	500	16.6	2500	-	1575	16.6
83196	500	16.6	2500	-	1600	16.6
83197	500	16.6	2500	-	1625	16.6
83198	500	16.6	2500	-	1650	16.6
83025	600	16.3	3000	-	2100	16.3
83026	600	16.3	3000	-	2130	16.3
83027	600	16.3	3000	-	2160	16.3
83048	550	16.3	2750	-	270	16.3
82929	650	16.2	3250	-	1632	16.2
82930	650	16.2	3250	-	1664	16.2
82931	650	16.2	3250	-	1696	16.2
82932	650	16.2	3250	-	1728	16.2
82933	650	16.2	3250	-	1760	16.2
82934	650	16.2	3250	-	1792	16.2
82935	650	16.2	3250	-	1824	16.2
82936	650	16.2	3250	-	1856	16.2
82937	650	16.2	3250	-	1888	16.2
82938	650	16.2	3250	-	1920	16.2
82939	650	16.2	3250	-	1952	16.2
82940	650	16.2	3250	-	1984	16.2
82941	650	16.2	3250	-	2016	16.2
82942	650	16.2	3250	-	2048	16.2
82943	650	16.2	3250	-	2080	16.2
83186	500	16.2	2500	-	1350	16.2
82963	600	16.2	3000	-	240	16.2
83170	500	16.1	2500	-	950	16.1
83171	500	16.1	2500	-	975	16.1
83172	500	16.1	2500	-	1000	16.1
83173	500	16.1	2500	-	1025	16.1
83174	500	16.1	2500	-	1050	16.1
83175	500	16.1	2500	-	1075	16.1
83176	500	16.1	2500	-	1100	16.1
83177	500	16.1	2500	-	1125	16.1
83178	500	16.1	2500	-	1150	16.1
83179	500	16.1	2500	-	1175	16.1
83180	500	16.1	2500	-	1200	16.1
83181	500	16.1	2500	-	1225	16.1
83182	500	16.1	2500	-	1250	16.1
83183	500	16.1	2500	-	1275	16.1
83184	500	16.1	2500	-	1300	16.1
83185	500	16.1	2500	-	1325	16.1
83199	500	16.1	2500	-	1675	16.1
83200	500	16.1	2500	-	1700	16.1
83201	500	16.1	2500	-	1725	16.1
83119	550	16.0	2750	-	2187	16.0
83120	550	16.0	2750	-	2214	16.0
83121	550	16.0	2750	-	2241	16.0
83132	500	15.9	2500	-	0	15.9
83133	500	15.9	2500	-	25	15.9
83134	500	15.9	2500	-	50	15.9
83202	500	15.7	2500	-	1750	15.7
83168	500	16.0	2500	0.2	900	15.6
83203	500	15.5	2500	-	1775	15.5
82885	650	15.4	3250	-	224	15.4
83011	600	15.0	3000	-	1680	15.0
83012	600	15.0	3000	-	1710	15.0
83013	600	15.0	3000	-	1740	15.0
83014	600	15.0	3000	-	1770	15.0
83015	600	15.0	3000	-	1800	15.0
83016	600	15.0	3000	-	1830	15.0
83017	600	15.0	3000	-	1860	15.0
83018	600	15.0	3000	-	1890	15.0
83019	600	15.0	3000	-	1920	15.0
83020	600	15.0	3000	-	1950	15.0
83021	600	15.0	3000	-	1980	15.0
83022	600	15.0	3000	-	2010	15.0
83023	600	15.0	3000	-	2040	15.0
83024	600	15.0	3000	-	2070	15.0
83116	550	14.9	2750	-	2106	14.9
83117	550	14.9	2750	-	2133	14.9
83118	550	14.9	2750	-	2160	14.9
83145	500	14.8	2500	-	325	14.8
83220	500	14.5	2500	-	2200	14.5
83221	500	14.5	2500	-	2225	14.5
83222	500	14.5	2500	-	2250	14.5
82964	600	14.2	3000	-	270	14.2
83103	550	13.8	2750	-	1755	13.8
83104	550	13.8	2750	-	1782	13.8
83105	550	13.8	2750	-	1809	13.8
83106	550	13.8	2750	-	1836	13.8
83107	550	13.8	2750	-	1863	13.8
83108	550	13.8	2750	-	1890	13.8
83109	550	13.8	2750	-	1917	13.8
83110	550	13.8	2750	-	1944	13.8
83111	550	13.8	2750	-	1971	13.8
83112	550	13.8	2750	-	1998	13.8
83113	550	13.8	2750	-	2025	13.8
83114	550	13.8	2750	-	2052	13.8
83115	550	13.8	2750	-	2079	13.8
83216	500	13.6	2500	-	2100	13.6
83217	500	13.6	2500	-	2125	13.6
83218	500	13.6	2500	-	2150	13.6
83219	500	13.6	2500	-	2175	13.6
83049	550	13.0	2750	-	297	13.0
83050	550	13.0	2750	-	324	13.0
83204	500	12.5	2500	-	1800	12.5
83205	500	12.5	2500	-	1825	12.5
83206	500	12.5	2500	-	1850	12.5
83207	500	12.5	2500	-	1875	12.5
83208	500	12.5	2500	-	1900	12.5
83209	500	12.5	2500	-	1925	12.5
83210	500	12.5	2500	-	1950	12.5
83211	500	12.5	2500	-	1975	12.5
83212	500	12.5	2500	-	2000	12.5
83213	500	12.5	2500	-	2025	12.5
83214	500	12.5	2500	-	2050	12.5
83215	500	12.5	2500	-	2075	12.5
83146	500	11.8	2500	-	350	11.8
83147	500	11.8	2500	-	375	11.8

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
82336	1250	39.8	6250	1.8	806	36.3
82337	1250	39.8	6250	1.8	868	36.3
82366	1200	38.2	6000	1.8	780	34.5
82367	1200	38.2	6000	1.8	840	34.5
82399	1150	36.6	5750	1.9	798	32.8
82400	1150	36.6	5750	1.9	855	32.8
82435	1100	35.0	5500	1.9	880	31.2
82433	1100	35.0	5500	2.0	770	31.0
82434	1100	35.0	5500	2.0	825	31.0
82471	1050	33.4	5250	2.1	780	29.2
82472	1050	33.4	5250	2.1	832	29.2
82350	1250	31.2	6250	1.0	1674	29.2
82351	1250	31.2	6250	1.0	1736	29.2
82352	1250	31.2	6250	1.0	1798	29.2
82381	1200	30.0	6000	1.1	1680	27.8
82382	1200	30.0	6000	1.1	1740	27.8
82383	1200	30.0	6000	1.1	1800	27.8
82512	1000	31.8	5000	2.2	800	27.4
82513	1000	31.8	5000	2.2	850	27.4
82415	1150	28.8	5750	1.1	1710	26.5
82416	1150	28.8	5750	1.1	1767	26.5
82417	1150	28.8	5750	1.1	1824	26.5
82418	1150	28.8	5750	1.1	1881	26.5
82323	1250	29.6	6250	1.8	0	26.1
82328	1250	29.6	6250	1.8	310	26.1
82329	1250	29.6	6250	1.8	372	26.1
82330	1250	29.6	6250	1.8	434	26.1
82331	1250	29.6	6250	1.8	496	26.1
82332	1250	29.6	6250	1.8	558	26.1
82333	1250	29.6	6250	1.8	620	26.1
82334	1250	29.6	6250	1.8	682	26.1
82335	1250	29.6	6250	1.8	744	26.1
82555	950	30.3	4750	2.3	799	25.6
82556	950	30.3	4750	2.3	846	25.6
82451	1100	27.5	5500	1.2	1760	25.1
82452	1100	27.5	5500	1.2	1815	25.1
82453	1100	27.5	5500	1.2	1870	25.1
82454	1100	27.5	5500	1.2	1925	25.1
82353	1200	28.4	6000	1.8	0	24.8
82354	1200	28.4	6000	1.8	60	24.8
82355	1200	28.4	6000	1.8	120	24.8
82356	1200	28.4	6000	1.8	180	24.8
82357	1200	28.4	6000	1.8	240	24.8
82358	1200	28.4	6000	1.8	300	24.8
82359	1200	28.4	6000	1.8	360	24.8
82360	1200	28.4	6000	1.8	420	24.8
82361	1200	28.4	6000	1.8	480	24.8
82362	1200	28.4	6000	1.8	540	24.8
82363	1200	28.4	6000	1.8	600	24.8
82364	1200	28.4	6000	1.8	660	24.8
82365	1200	28.4	6000	1.8	720	24.8
82491	1050	26.2	5250	1.2	1820	23.8
82492	1050	26.2	5250	1.2	1872	23.8
82493	1050	26.2	5250	1.2	1924	23.8
82494	1050	26.2	5250	1.2	1976	23.8
82495	1050	26.2	5250	1.2	2028	23.8
82601	900	28.7	4500	2.4	765	23.8
82602	900	28.7	4500	2.4	810	23.8
82603	900	28.7	4500	2.4	855	23.8
82385	1150	27.3	5750	1.9	0	23.4
82386	1150	27.3	5750	1.9	57	23.4
82387	1150	27.3	5750	1.9	114	23.4
82388	1150	27.3	5750	1.9	171	23.4
82389	1150	27.3	5750	1.9	228	23.4
82390	1150	27.3	5750	1.9	285	23.4
82391	1150	27.3	5750	1.9	342	23.4
82392	1150	27.3	5750	1.9	399	23.4
82393	1150	27.3	5750	1.9	456	23.4
82394	1150	27.3	5750	1.9	513	23.4
82395	1150	27.3	5750	1.9	570	23.4
82396	1150	27.3	5750	1.9	627	23.4
82397	1150	27.3	5750	1.9	684	23.4
82398	1150	27.3	5750	1.9	741	23.4
82583	950	25.8	4750	1.4	2115	23.1
82654	850	27.1	4250	2.2	882	22.6
82533	1000	25.0	5000	1.3	1850	22.4
82534	1000	25.0	5000	1.3	1900	22.4
82535	1000	25.0	5000	1.3	1950	22.4
82536	1000	25.0	5000	1.3	2000	22.4
82537	1000	25.0	5000	1.3	2050	22.4
82419	1100	26.1	5500	2.0	0	22.1
82420	1100	26.1	5500	2.0	55	22.1
82421	1100	26.1	5500	2.0	110	22.1
82422	1100	26.1	5500	2.0	165	22.1
82423	1100	26.1	5500	2.0	220	22.1
82424	1100	26.1	5500	2.0	275	22.1
82425	1100	26.1	5500	2.0	330	22.1
82426	1100	26.1	5500	2.0	385	22.1
82427	1100	26.1	5500	2.0	440	22.1
82428	1100	26.1	5500	2.0	495	22.1
82429	1100	26.1	5500	2.0	550	22.1
82430	1100	26.1	5500	2.0	605	22.1
82431	1100	26.1	5500	2.0	660	22.1
82432	1100	26.1	5500	2.0	715	22.1
82652	850	27.1	4250	2.6	798	21.9
82653	850	27.1	4250	2.6	840	21.9
82685	850	24.7	4250	1.5	2184	21.7
82686	850	24.7	4250	1.5	2226	21.7
82631	900	24.5	4500	1.4	2115	21.6
82632	900	24.5	4500	1.4	2160	21.6
82579	950	23.8	4750	1.4	1927	21.0
82580	950	23.8	4750	1.4	1974	21.0
82581	950	23.8	4750	1.4	2021	21.0
82582	950	23.8	4750	1.4	2068	21.0
82456	1050	24.9	5250	2.1	0	20.7
82457	1050	24.9	5250	2.1	52	20.7
82458	1050	24.9	5250	2.1	104	20.7
82459	1050	24.9	5250	2.1	156	20.7
82460	1050	24.9	5250	2.1	208	20.7
82461	1050	24.9	5250	2.1	260	20.7
82462	1050	24.9	5250	2.1	312	20.7
82463	1050	24.9	5250	2.1	364	20.7
82464	1050	24.9	5250	2.1	416	20.7
82465	1050	24.9	5250	2.1	468	20.7
82466	1050	24.9	5250	2.1	520	20.7
82467	1050	24.9	5250	2.1	572	20.7
82468	1050	24.9	5250	2.1	624	20.7
82469	1050	24.9	5250	2.1	676	20.7
82470	1050	24.9	5250	2.1	728	20.7
82769	750	24.0	3750	1.7	888	20.6
82806	750	23.9	3750	1.7	2257	20.4
82709	800	25.5	4000	2.6	880	20.2
82683	850	23.1	4250	1.5	2100	20.0
82684	850	23.1	4250	1.5	2142	20.0
82742	800	23.3	4000	1.6	2200	20.0
82743	800	23.3	4000	1.6	2240	20.0
82707	800	25.5	4000	2.8	800	20.0
82708	800	25.5	4000	2.8	840	20.0
82628	900	22.5	4500	1.4	1980	19.6
82629	900	22.5	4500	1.4	2025	19.6
82630	900	22.5	4500	1.4	2070	19.6
82496	1000	23.7	5000	2.2	0	19.3
82497	1000	23.7	5000	2.2	50	19.3
82498	1000	23.7	5000	2.2	100	19.3
82499	1000	23.7	5000	2.2	150	19.3
82500	1000	23.7	5000	2.2	200	19.3
82501	1000	23.7	5000	2.2	250	19.3
82502	1000	23.7	5000	2.2	300	19.3
82503	1000	23.7	5000	2.2	350	19.3
82504	1000	23.7	5000	2.2	400	19.3
82505	1000	23.7	5000	2.2	450	19.3
82506	1000	23.7	5000	2.2	500	19.3
82507	1000	23.7	5000	2.2	550	19.3
82508	1000	23.7	5000	2.2	600	19.3
82509	1000	23.7	5000	2.2	650	19.3
82510	1000	23.7	5000	2.2	700	19.3
82511	1000	23.7	5000	2.2	750	19.3
82740	800	21.7	4000	1.6	2120	18.5
82741	800	21.7	4000	1.6	2160	18.5
82804	750	21.8	3750	1.7	2183	18.3
82805	750	21.8	3750	1.7	2220	18.3
82681	850	21.2	4250	1.5	2016	18.2
82682	850	21.2	4250	1.5	2058	18.2
82766	750	23.9	3750	2.9	777	18.0
82767	750	23.9	3750	2.9	814	18.0
82768	750	23.9	3750	2.9	851	18.0
82538	950	22.5	4750	2.3	0	17.9
82539	950	22.5	4750	2.3	47	17.9
82540	950	22.5	4750	2.3	94	17.9
82541	950	22.5	4750	2.3	141	17.9
82542	950	22.5	4750	2.3	188	17.9
82543	950	22.5	4750	2.3	235	17.9
82544	950	22.5	4750	2.3	282	17.9
82545	950	22.5	4750	2.3	329	17.9
82546	950	22.5	4750	2.3	376	17.9
82547	950	22.5	4750	2.3	423	17.9
82548	950	22.5	4750	2.3	470	17.9
82549	950	22.5	4750	2.3	517	17.9
82550	950	22.5	4750	2.3	564	17.9
82551	950	22.5	4750	2.3	611	17.9
82552	950	22.5	4750	2.3	658	17.9
82553	950	22.5	4750	2.3	705	17.9
82554	950	22.5	4750	2.3	752	17.9
82802	750	20.4	3750	1.7	2109	16.9
82803	750	20.4	3750	1.7	2146	16.9
82739	800	20.0	4000	1.6	2080	16.8
82584	900	21.3	4500	2.3	0	16.7
82872	700	20.3	3500	1.9	2205	16.6
82873	700	20.3	3500	1.9	2240	16.6
82585	900	21.3	4500	2.4	45	16.4
82586	900	21.3	4500	2.4	90	16.4
82587	900	21.3	4500	2.4	135	16.4
82588	900	21.3	4500	2.4	180	16.4
82589	900	21.3	4500	2.4	225	16.4
82590	900	21.3	4500	2.4	270	16.4
82591	900	21.3	4500	2.4	315	16.4
82592	900	21.3	4500	2.4	360	16.4
82593	900	21.3	4500	2.4	405	16.4
82594	900	21.3	4500	2.4	450	16.4
82595	900	21.3	4500	2.4	495	16.4
82596	900	21.3	4500	2.4	540	16.4
82597	900	21.3	4500	2.4	585	16.4
82598	900	21.3	4500	2.4	630	16.4
82599	900	21.3	4500	2.4	675	16.4
82600	900	21.3	4500	2.4	720	16.4
82831	700	22.3	3500	3.1	770	16.0
82832	700	22.3	3500	3.1	805	16.0
82833	700	22.3	3500	3.1	840	16.0
82834	700	22.3	3500	3.1	875	16.0
82871	700	19.0	3500	1.9	2170	15.3
82651	850	20.2	4250	2.6	756	15.1
82635	850	20.1	4250	2.6	84	15.0
82636	850	20.1	4250	2.6	126	15.0
82637	850	20.1	4250	2.6	168	15.0
82638	850	20.1	4250	2.6	210	15.0
82639	850	20.1	4250	2.6	252	15.0
82640	850	20.1	4250	2.6	294	15.0
82641	850	20.1	4250	2.6	336	15.0
82642	850	20.1	4250	2.6	378	15.0
82643	850	20.1	4250	2.6	420	15.0
82644	850	20.1	4250	2.6	462	15.0
82645	850	20.1	4250	2.6	504	15.0
82646	850	20.1	4250	2.6	546	15.0
82647	850	20.1	4250	2.6	588	15.0
82648	850	20.1	4250	2.6	630	15.0
82649	850	20.1	4250	2.6	672	15.0
82650	850	20.1	4250	2.6	714	15.0
82947	650	18.9	3250	2.0	2208	14.9
82948	650	18.9	3250	2.0	2240	14.9
83071	550	17.6	2750	1.8	891	14.0
82902	650	20.7	3250	3.4	768	13.9
82903	650	20.7	3250	3.4	800	13.9
82904	650	20.7	3250	3.4	832	13.9
82905	650	20.7	3250	3.4	864	13.9
82706	800	19.3	4000	2.8	760	13.8
82690	800	19.0	4000	2.8	120	13.5
82691	800	19.0	4000	2.8	160	13.5
82692	800	19.0	4000	2.8	200	13.5
82693	800	19.0	4000	2.8	240	13.5
82694	800	19.0	4000	2.8	280	13.5
82695	800	19.0	4000	2.8	320	13.5
82696	800	19.0	4000	2.8	360	13.5
82697	800	19.0	4000	2.8	400	13.5
82698	800	19.0	4000	2.8	440	13.5
82699	800	19.0	4000	2.8	480	13.5
82700	800	19.0	4000	2.8	520	13.5
82701	800	19.0	4000	2.8	560	13.5
82702	800	19.0	4000	2.8	600	13.5
82703	800	19.0	4000	2.8	640	13.5
82704	800	19.0	4000	2.8	680	13.5
82705	800	19.0	4000	2.8	720	13.5
83030	600	17.4	3000	2.2	2250	13.1
82749	750	17.8	3750	2.5	148	12.7
82750	750	17.8	3750	2.9	185	11.9
82751	750	17.8	3750	2.9	222	11.9
82752	750	17.8	3750	2.9	259	11.9
82753	750	17.8	3750	2.9	296	11.9
82754	750	17.8	3750	2.9	333	11.9
82755	750	17.8	3750	2.9	370	11.9
82756	750	17.8	3750	2.9	407	11.9
82757	750	17.8	3750	2.9	444	11.9
82758	750	17.8	3750	2.9	481	11.9
82759	750	17.8	3750	2.9	518	11.9
82760	750	17.8	3750	2.9	555	11.9
82761	750	17.8	3750	2.9	592	11.9
82762	750	17.8	3750	2.9	629	11.9
82763	750	17.8	3750	2.9	666	11.9
82764	750	17.8	3750	2.9	703	11.9
82765	750	17.8	3750	2.9	740	11.9
82981	600	19.1	3000	3.7	780	11.8
82982	600	19.1	3000	3.7	810	11.8
82983	600	19.1	3000	3.7	840	11.8
82984	600	19.1	3000	3.7	870	11.8
82815	700	16.6	3500	3.1	210	10.3
82816	700	16.6	3500	3.1	245	10.3
82817	700	16.6	3500	3.1	280	10.3
82818	700	16.6	3500	3.1	315	10.3
82819	700	16.6	3500	3.1	350	10.3
82820	700	16.6	3500	3.1	385	10.3
82821	700	16.6	3500	3.1	420	10.3
82822	700	16.6	3500	3.1	455	10.3
82823	700	16.6	3500	3.1	490	10.3
82824	700	16.6	3500	3.1	525	10.3
82825	700	16.6	3500	3.1	560	10.3
82826	700	16.6	3500	3.1	595	10.3
82827	700	16.6	3500	3.1	630	10.3
82828	700	16.6	3500	3.1	665	10.3
82829	700	16.6	3500	3.1	700	10.3
82830	700	16.6	3500	3.1	735	10.3
83067	550	17.5	2750	4.0	783	9.5
83068	550	17.5	2750	4.0	810	9.5
83069	550	17.5	2750	4.0	837	9.5
83070	550	17.5	2750	4.0	864	9.5
82886	650	15.4	3250	3.4	256	8.6
82887	650	15.4	3250	3.4	288	8.6
82888	650	15.4	3250	3.4	320	8.6
82889	650	15.4	3250	3.4	352	8.6
82890	650	15.4	3250	3.4	384	8.6
82891	650	15.4	3250	3.4	416	8.6
82892	650	15.4	3250	3.4	448	8.6
82893	650	15.4	3250	3.4	480	8.6
82894	650	15.4	3250	3.4	512	8.6
82895	650	15.4	3250	3.4	544	8.6
82896	650	15.4	3250	3.4	576	8.6
82897	650	15.4	3250	3.4	608	8.6
82898	650	15.4	3250	3.4	640	8.6
82899	650	15.4	3250	3.4	672	8.6
82900	650	15.4	3250	3.4	704	8.6
82901	650	15.4	3250	3.4	736	8.6
82965	600	14.2	3000	3.5	300	7.2
83163	500	15.9	2500	4.4	775	7.1
83164	500	15.9	2500	4.4	800	7.1
83165	500	15.9	2500	4.4	825	7.1
83166	500	15.9	2500	4.4	850	7.1
83167	500	15.9	2500	4.4	875	7.1
82966	600	14.2	3000	3.7	330	6.9
82967	600	14.2	3000	3.7	360	6.9
82968	600	14.2	3000	3.7	390	6.9
82969	600	14.2	3000	3.7	420	6.9
82970	600	14.2	3000	3.7	450	6.9
82971	600	14.2	3000	3.7	480	6.9
82972	600	14.2	3000	3.7	510	6.9
82973	600	14.2	3000	3.7	540	6.9
82974	600	14.2	3000	3.7	570	6.9
82975	600	14.2	3000	3.7	600	6.9
82976	600	14.2	3000	3.7	630	6.9
82977	600	14.2	3000	3.7	660	6.9
82978	600	14.2	3000	3.7	690	6.9
82979	600	14.2	3000	3.7	720	6.9
82980	600	14.2	3000	3.7	750	6.9
83066	550	13.1	2750	4.0	756	5.1
83051	550	13.0	2750	4.0	351	5.0
83052	550	13.0	2750	4.0	378	5.0
83053	550	13.0	2750	4.0	405	5.0
83054	550	13.0	2750	4.0	432	5.0
83055	550	13.0	2750	4.0	459	5.0
83056	550	13.0	2750	4.0	486	5.0
83057	550	13.0	2750	4.0	513	5.0
83058	550	13.0	2750	4.0	540	5.0
83059	550	13.0	2750	4.0	567	5.0
83060	550	13.0	2750	4.0	594	5.0
83061	550	13.0	2750	4.0	621	5.0
83062	550	13.0	2750	4.0	648	5.0
83063	550	13.0	2750	4.0	675	5.0
83064	550	13.0	2750	4.0	702	5.0
83065	550	13.0	2750	4.0	729	5.0
83148	500	11.8	2500	4.2	400	3.4
83149	500	11.8	2500	4.4	425	3.0
83150	500	11.8	2500	4.4	450	3.0
83151	500	11.8	2500	4.4	475	3.0
83152	500	11.8	2500	4.4	500	3.0
83153	500	11.8	2500	4.4	525	3.0
83154	500	11.8	2500	4.4	550	3.0
83155	500	11.8	2500	4.4	575	3.0
83156	500	11.8	2500	4.4	600	3.0
83157	500	11.8	2500	4.4	625	3.0
83158	500	11.8	2500	4.4	650	3.0
83159	500	11.8	2500	4.4	675	3.0
83160	500	11.8	2500	4.4	700	3.0
83161	500	11.8	2500	4.4	725	3.0
83162	500	11.8	2500	4.4	750	3.0

Overall score = score_ratio - 2 * repetitive DNA content