Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign109 - back to list

Summary
Image
Unique putative probes (79)
Putative probes (421)
Redundant probes (3)

Summary

`./analyse_probe_search_results : Fri Feb 17 09:53:40 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign109
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 5
  id            : 105
  strand        : 1
  -------------

Design window length: 2501bp

All jobs for probe design are successful - total: 35
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 700 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 700/700
Putative probes that are unique                 : 79/700
Putative probes exceeding selection criteria    : 503/700 (both unique and not unique)
Putative probes with successfully-picked primers: 497/503

Identified 3 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
100198	950	4750	-	1551
100234	900	4500	-	1575
100272	850	4250	-	1554
100273	850	4250	-	1596
100274	850	4250	-	1638
100314	800	4000	-	1560
100315	800	4000	-	1600
100316	800	4000	-	1640
100317	800	4000	-	1680
100360	750	3750	-	1554
100361	750	3750	-	1591
100362	750	3750	-	1628
100363	750	3750	-	1665
100364	750	3750	-	1702
100365	750	3750	-	1739
100410	700	3500	-	1540
100411	700	3500	-	1575
100412	700	3500	-	1610
100413	700	3500	-	1645
100414	700	3500	-	1680
100415	700	3500	-	1715
100416	700	3500	-	1750
100417	700	3500	-	1785
100467	650	3250	-	1568
100468	650	3250	-	1600
100469	650	3250	-	1632
100470	650	3250	-	1664
100471	650	3250	-	1696
100472	650	3250	-	1728
100473	650	3250	-	1760
100474	650	3250	-	1792
100475	650	3250	-	1824
100528	600	3000	-	1560
100529	600	3000	-	1590
100530	600	3000	-	1620
100531	600	3000	-	1650
100532	600	3000	-	1680
100533	600	3000	-	1710
100534	600	3000	-	1740
100535	600	3000	-	1770
100536	600	3000	-	1800
100537	600	3000	-	1830
100538	600	3000	-	1860
100539	600	3000	-	1890
100597	550	2750	-	1539
100598	550	2750	-	1566
100599	550	2750	-	1593
100600	550	2750	-	1620
100601	550	2750	-	1647
100602	550	2750	-	1674
100603	550	2750	-	1701
100604	550	2750	-	1728
100605	550	2750	-	1755
100606	550	2750	-	1782
100607	550	2750	-	1809
100608	550	2750	-	1836
100609	550	2750	-	1863
100610	550	2750	-	1890
100611	550	2750	-	1917
100612	550	2750	-	1944
100675	500	2500	-	1550
100676	500	2500	-	1575
100677	500	2500	-	1600
100678	500	2500	-	1625
100679	500	2500	-	1650
100680	500	2500	-	1675
100681	500	2500	-	1700
100682	500	2500	-	1725
100683	500	2500	-	1750
100684	500	2500	-	1775
100685	500	2500	-	1800
100686	500	2500	-	1825
100687	500	2500	-	1850
100688	500	2500	-	1875
100689	500	2500	-	1900
100690	500	2500	-	1925
100691	500	2500	-	1950
100692	500	2500	-	1975
100693	500	2500	-	2000

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
100002	1300	38.7	6500	-	520	38.7
100003	1300	38.7	6500	-	585	38.7
100004	1300	38.7	6500	-	650	38.7
100005	1300	38.7	6500	-	715	38.7
100006	1300	38.7	6500	-	780	38.7
100007	1300	38.7	6500	-	845	38.7
100008	1300	38.7	6500	-	910	38.7
100009	1300	38.7	6500	-	975	38.7
100010	1300	38.7	6500	-	1040	38.7
100011	1300	38.7	6500	-	1105	38.7
100012	1300	38.7	6500	-	1170	38.7
100022	1250	37.2	6250	-	558	37.2
100023	1250	37.2	6250	-	620	37.2
100024	1250	37.2	6250	-	682	37.2
100025	1250	37.2	6250	-	744	37.2
100026	1250	37.2	6250	-	806	37.2
100027	1250	37.2	6250	-	868	37.2
100028	1250	37.2	6250	-	930	37.2
100029	1250	37.2	6250	-	992	37.2
100030	1250	37.2	6250	-	1054	37.2
100031	1250	37.2	6250	-	1116	37.2
100032	1250	37.2	6250	-	1178	37.2
100033	1250	37.2	6250	-	1240	37.2
100021	1250	37.2	6250	0.2	496	36.7
100043	1200	35.7	6000	-	540	35.7
100044	1200	35.7	6000	-	600	35.7
100045	1200	35.7	6000	-	660	35.7
100046	1200	35.7	6000	-	720	35.7
100047	1200	35.7	6000	-	780	35.7
100048	1200	35.7	6000	-	840	35.7
100049	1200	35.7	6000	-	900	35.7
100050	1200	35.7	6000	-	960	35.7
100051	1200	35.7	6000	-	1020	35.7
100052	1200	35.7	6000	-	1080	35.7
100053	1200	35.7	6000	-	1140	35.7
100054	1200	35.7	6000	-	1200	35.7
100055	1200	35.7	6000	-	1260	35.7
100065	1150	34.2	5750	-	513	34.2
100066	1150	34.2	5750	-	570	34.2
100067	1150	34.2	5750	-	627	34.2
100068	1150	34.2	5750	-	684	34.2
100069	1150	34.2	5750	-	741	34.2
100070	1150	34.2	5750	-	798	34.2
100071	1150	34.2	5750	-	855	34.2
100072	1150	34.2	5750	-	912	34.2
100073	1150	34.2	5750	-	969	34.2
100074	1150	34.2	5750	-	1026	34.2
100075	1150	34.2	5750	-	1083	34.2
100076	1150	34.2	5750	-	1140	34.2
100077	1150	34.2	5750	-	1197	34.2
100078	1150	34.2	5750	-	1254	34.2
100079	1150	34.2	5750	-	1311	34.2
100090	1100	32.7	5500	-	550	32.7
100091	1100	32.7	5500	-	605	32.7
100092	1100	32.7	5500	-	660	32.7
100093	1100	32.7	5500	-	715	32.7
100094	1100	32.7	5500	-	770	32.7
100095	1100	32.7	5500	-	825	32.7
100096	1100	32.7	5500	-	880	32.7
100097	1100	32.7	5500	-	935	32.7
100098	1100	32.7	5500	-	990	32.7
100099	1100	32.7	5500	-	1045	32.7
100100	1100	32.7	5500	-	1100	32.7
100101	1100	32.7	5500	-	1155	32.7
100102	1100	32.7	5500	-	1210	32.7
100103	1100	32.7	5500	-	1265	32.7
100104	1100	32.7	5500	-	1320	32.7
100105	1100	32.7	5500	-	1375	32.7
100042	1200	35.7	6000	1.6	480	32.5
100089	1100	32.7	5500	0.4	495	32.0
100116	1050	31.6	5250	-	520	31.6
100117	1050	31.2	5250	-	572	31.2
100118	1050	31.2	5250	-	624	31.2
100119	1050	31.2	5250	-	676	31.2
100120	1050	31.2	5250	-	728	31.2
100121	1050	31.2	5250	-	780	31.2
100122	1050	31.2	5250	-	832	31.2
100123	1050	31.2	5250	-	884	31.2
100124	1050	31.2	5250	-	936	31.2
100125	1050	31.2	5250	-	988	31.2
100126	1050	31.2	5250	-	1040	31.2
100127	1050	31.2	5250	-	1092	31.2
100128	1050	31.2	5250	-	1144	31.2
100129	1050	31.2	5250	-	1196	31.2
100130	1050	31.2	5250	-	1248	31.2
100131	1050	31.2	5250	-	1300	31.2
100132	1050	31.2	5250	-	1352	31.2
100133	1050	31.2	5250	-	1404	31.2
100144	1000	30.1	5000	-	500	30.1
100145	1000	30.1	5000	-	550	30.1
100146	1000	29.8	5000	-	600	29.8
100147	1000	29.8	5000	-	650	29.8
100148	1000	29.8	5000	-	700	29.8
100149	1000	29.8	5000	-	750	29.8
100150	1000	29.8	5000	-	800	29.8
100151	1000	29.8	5000	-	850	29.8
100152	1000	29.8	5000	-	900	29.8
100153	1000	29.8	5000	-	950	29.8
100154	1000	29.8	5000	-	1000	29.8
100155	1000	29.8	5000	-	1050	29.8
100156	1000	29.8	5000	-	1100	29.8
100157	1000	29.8	5000	-	1150	29.8
100158	1000	29.8	5000	-	1200	29.8
100159	1000	29.8	5000	-	1250	29.8
100160	1000	29.8	5000	-	1300	29.8
100161	1000	29.8	5000	-	1350	29.8
100162	1000	29.8	5000	-	1400	29.8
100163	1000	29.8	5000	-	1450	29.8
100164	1000	29.8	5000	-	1500	29.8
100176	950	28.6	4750	-	517	28.6
100177	950	28.6	4750	-	564	28.6
100178	950	28.6	4750	-	611	28.6
100179	950	28.3	4750	-	658	28.3
100180	950	28.3	4750	-	705	28.3
100181	950	28.3	4750	-	752	28.3
100182	950	28.3	4750	-	799	28.3
100183	950	28.3	4750	-	846	28.3
100184	950	28.3	4750	-	893	28.3
100185	950	28.3	4750	-	940	28.3
100186	950	28.3	4750	-	987	28.3
100187	950	28.3	4750	-	1034	28.3
100188	950	28.3	4750	-	1081	28.3
100189	950	28.3	4750	-	1128	28.3
100190	950	28.3	4750	-	1175	28.3
100191	950	28.3	4750	-	1222	28.3
100192	950	28.3	4750	-	1269	28.3
100193	950	28.3	4750	-	1316	28.3
100194	950	28.3	4750	-	1363	28.3
100195	950	28.3	4750	-	1410	28.3
100196	950	28.3	4750	-	1457	28.3
100197	950	28.3	4750	-	1504	28.3
100211	900	27.1	4500	-	540	27.1
100212	900	27.1	4500	-	585	27.1
100213	900	27.1	4500	-	630	27.1
100214	900	27.1	4500	-	675	27.1
100215	900	26.8	4500	-	720	26.8
100216	900	26.8	4500	-	765	26.8
100217	900	26.8	4500	-	810	26.8
100218	900	26.8	4500	-	855	26.8
100219	900	26.8	4500	-	900	26.8
100220	900	26.8	4500	-	945	26.8
100221	900	26.8	4500	-	990	26.8
100222	900	26.8	4500	-	1035	26.8
100223	900	26.8	4500	-	1080	26.8
100224	900	26.8	4500	-	1125	26.8
100225	900	26.8	4500	-	1170	26.8
100226	900	26.8	4500	-	1215	26.8
100227	900	26.8	4500	-	1260	26.8
100228	900	26.8	4500	-	1305	26.8
100229	900	26.8	4500	-	1350	26.8
100230	900	26.8	4500	-	1395	26.8
100231	900	26.8	4500	-	1440	26.8
100232	900	26.8	4500	-	1485	26.8
100233	900	26.8	4500	-	1530	26.8
100210	900	27.1	4500	0.4	495	26.2
100247	850	25.6	4250	-	504	25.6
100248	850	25.6	4250	-	546	25.6
100249	850	25.6	4250	-	588	25.6
100250	850	25.6	4250	-	630	25.6
100251	850	25.6	4250	-	672	25.6
100252	850	25.6	4250	-	714	25.6
100253	850	25.3	4250	-	756	25.3
100254	850	25.3	4250	-	798	25.3
100255	850	25.3	4250	-	840	25.3
100256	850	25.3	4250	-	882	25.3
100257	850	25.3	4250	-	924	25.3
100258	850	25.3	4250	-	966	25.3
100259	850	25.3	4250	-	1008	25.3
100260	850	25.3	4250	-	1050	25.3
100261	850	25.3	4250	-	1092	25.3
100262	850	25.3	4250	-	1134	25.3
100263	850	25.3	4250	-	1176	25.3
100264	850	25.3	4250	-	1218	25.3
100265	850	25.3	4250	-	1260	25.3
100266	850	25.3	4250	-	1302	25.3
100267	850	25.3	4250	-	1344	25.3
100268	850	25.3	4250	-	1386	25.3
100269	850	25.3	4250	-	1428	25.3
100270	850	25.3	4250	-	1470	25.3
100271	850	25.3	4250	-	1512	25.3
100288	800	24.1	4000	-	520	24.1
100289	800	24.1	4000	-	560	24.1
100290	800	24.1	4000	-	600	24.1
100291	800	24.1	4000	-	640	24.1
100292	800	24.1	4000	-	680	24.1
100293	800	24.1	4000	-	720	24.1
100294	800	24.1	4000	-	760	24.1
100295	800	23.8	4000	-	800	23.8
100296	800	23.8	4000	-	840	23.8
100297	800	23.8	4000	-	880	23.8
100298	800	23.8	4000	-	920	23.8
100299	800	23.8	4000	-	960	23.8
100300	800	23.8	4000	-	1000	23.8
100301	800	23.8	4000	-	1040	23.8
100302	800	23.8	4000	-	1080	23.8
100303	800	23.8	4000	-	1120	23.8
100304	800	23.8	4000	-	1160	23.8
100305	800	23.8	4000	-	1200	23.8
100306	800	23.8	4000	-	1240	23.8
100307	800	23.8	4000	-	1280	23.8
100308	800	23.8	4000	-	1320	23.8
100309	800	23.8	4000	-	1360	23.8
100310	800	23.8	4000	-	1400	23.8
100311	800	23.8	4000	-	1440	23.8
100312	800	23.8	4000	-	1480	23.8
100313	800	23.8	4000	-	1520	23.8
100332	750	22.6	3750	-	518	22.6
100333	750	22.6	3750	-	555	22.6
100334	750	22.6	3750	-	592	22.6
100335	750	22.6	3750	-	629	22.6
100336	750	22.6	3750	-	666	22.6
100337	750	22.6	3750	-	703	22.6
100338	750	22.6	3750	-	740	22.6
100339	750	22.6	3750	-	777	22.6
100340	750	22.6	3750	-	814	22.6
100341	750	22.3	3750	-	851	22.3
100342	750	22.3	3750	-	888	22.3
100343	750	22.3	3750	-	925	22.3
100344	750	22.3	3750	-	962	22.3
100345	750	22.3	3750	-	999	22.3
100346	750	22.3	3750	-	1036	22.3
100347	750	22.3	3750	-	1073	22.3
100348	750	22.3	3750	-	1110	22.3
100349	750	22.3	3750	-	1147	22.3
100350	750	22.3	3750	-	1184	22.3
100351	750	22.3	3750	-	1221	22.3
100352	750	22.3	3750	-	1258	22.3
100353	750	22.3	3750	-	1295	22.3
100354	750	22.3	3750	-	1332	22.3
100355	750	22.3	3750	-	1369	22.3
100356	750	22.3	3750	-	1406	22.3
100357	750	22.3	3750	-	1443	22.3
100358	750	22.3	3750	-	1480	22.3
100359	750	22.3	3750	-	1517	22.3
100115	1050	28.2	5250	3.0	468	22.3
100001	1300	28.0	6500	3.4	455	21.2
100381	700	21.1	3500	-	525	21.1
100382	700	21.1	3500	-	560	21.1
100383	700	21.1	3500	-	595	21.1
100384	700	21.1	3500	-	630	21.1
100385	700	21.1	3500	-	665	21.1
100386	700	21.1	3500	-	700	21.1
100387	700	21.1	3500	-	735	21.1
100388	700	21.1	3500	-	770	21.1
100389	700	21.1	3500	-	805	21.1
100390	700	21.1	3500	-	840	21.1
100391	700	21.1	3500	-	875	21.1
100392	700	20.8	3500	-	910	20.8
100393	700	20.8	3500	-	945	20.8
100394	700	20.8	3500	-	980	20.8
100395	700	20.8	3500	-	1015	20.8
100396	700	20.8	3500	-	1050	20.8
100397	700	20.8	3500	-	1085	20.8
100398	700	20.8	3500	-	1120	20.8
100399	700	20.8	3500	-	1155	20.8
100400	700	20.8	3500	-	1190	20.8
100401	700	20.8	3500	-	1225	20.8
100402	700	20.8	3500	-	1260	20.8
100403	700	20.8	3500	-	1295	20.8
100404	700	20.8	3500	-	1330	20.8
100405	700	20.8	3500	-	1365	20.8
100406	700	20.8	3500	-	1400	20.8
100407	700	20.8	3500	-	1435	20.8
100408	700	20.8	3500	-	1470	20.8
100409	700	20.8	3500	-	1505	20.8
100434	650	20.2	3250	-	512	20.2
100175	950	26.2	4750	3.1	470	20.1
100435	650	19.6	3250	-	544	19.6
100436	650	19.6	3250	-	576	19.6
100437	650	19.6	3250	-	608	19.6
100438	650	19.6	3250	-	640	19.6
100439	650	19.6	3250	-	672	19.6
100440	650	19.6	3250	-	704	19.6
100441	650	19.6	3250	-	736	19.6
100442	650	19.6	3250	-	768	19.6
100443	650	19.6	3250	-	800	19.6
100444	650	19.6	3250	-	832	19.6
100445	650	19.6	3250	-	864	19.6
100446	650	19.6	3250	-	896	19.6
100447	650	19.6	3250	-	928	19.6
100287	800	24.1	4000	2.4	480	19.3
100448	650	19.3	3250	-	960	19.3
100449	650	19.3	3250	-	992	19.3
100450	650	19.3	3250	-	1024	19.3
100451	650	19.3	3250	-	1056	19.3
100452	650	19.3	3250	-	1088	19.3
100453	650	19.3	3250	-	1120	19.3
100454	650	19.3	3250	-	1152	19.3
100455	650	19.3	3250	-	1184	19.3
100456	650	19.3	3250	-	1216	19.3
100457	650	19.3	3250	-	1248	19.3
100458	650	19.3	3250	-	1280	19.3
100459	650	19.3	3250	-	1312	19.3
100460	650	19.3	3250	-	1344	19.3
100461	650	19.3	3250	-	1376	19.3
100462	650	19.3	3250	-	1408	19.3
100463	650	19.3	3250	-	1440	19.3
100464	650	19.3	3250	-	1472	19.3
100465	650	19.3	3250	-	1504	19.3
100466	650	19.3	3250	-	1536	19.3
100493	600	18.6	3000	-	510	18.6
100494	600	18.6	3000	-	540	18.6
100380	700	21.1	3500	1.3	490	18.5
100495	600	18.1	3000	-	570	18.1
100496	600	18.1	3000	-	600	18.1
100497	600	18.1	3000	-	630	18.1
100498	600	18.1	3000	-	660	18.1
100499	600	18.1	3000	-	690	18.1
100500	600	18.1	3000	-	720	18.1
100501	600	18.1	3000	-	750	18.1
100502	600	18.1	3000	-	780	18.1
100503	600	18.1	3000	-	810	18.1
100504	600	18.1	3000	-	840	18.1
100505	600	18.1	3000	-	870	18.1
100506	600	18.1	3000	-	900	18.1
100507	600	18.1	3000	-	930	18.1
100508	600	18.1	3000	-	960	18.1
100509	600	18.1	3000	-	990	18.1
100510	600	17.9	3000	-	1020	17.9
100511	600	17.9	3000	-	1050	17.9
100512	600	17.9	3000	-	1080	17.9
100513	600	17.9	3000	-	1110	17.9
100514	600	17.9	3000	-	1140	17.9
100515	600	17.9	3000	-	1170	17.9
100516	600	17.9	3000	-	1200	17.9
100517	600	17.9	3000	-	1230	17.9
100518	600	17.9	3000	-	1260	17.9
100519	600	17.9	3000	-	1290	17.9
100520	600	17.9	3000	-	1320	17.9
100521	600	17.9	3000	-	1350	17.9
100522	600	17.9	3000	-	1380	17.9
100523	600	17.9	3000	-	1410	17.9
100524	600	17.9	3000	-	1440	17.9
100525	600	17.9	3000	-	1470	17.9
100526	600	17.9	3000	-	1500	17.9
100527	600	17.9	3000	-	1530	17.9
100331	750	22.6	3750	2.4	481	17.8
100559	550	17.1	2750	-	513	17.1
100560	550	17.1	2750	-	540	17.1
100561	550	17.1	2750	-	567	17.1
100562	550	17.1	2750	-	594	17.1
100563	550	16.6	2750	-	621	16.6
100564	550	16.6	2750	-	648	16.6
100565	550	16.6	2750	-	675	16.6
100566	550	16.6	2750	-	702	16.6
100567	550	16.6	2750	-	729	16.6
100568	550	16.6	2750	-	756	16.6
100569	550	16.6	2750	-	783	16.6
100570	550	16.6	2750	-	810	16.6
100571	550	16.6	2750	-	837	16.6
100572	550	16.6	2750	-	864	16.6
100573	550	16.6	2750	-	891	16.6
100574	550	16.6	2750	-	918	16.6
100575	550	16.6	2750	-	945	16.6
100576	550	16.6	2750	-	972	16.6
100577	550	16.6	2750	-	999	16.6
100578	550	16.6	2750	-	1026	16.6
100579	550	16.4	2750	-	1053	16.4
100580	550	16.4	2750	-	1080	16.4
100581	550	16.4	2750	-	1107	16.4
100582	550	16.4	2750	-	1134	16.4
100583	550	16.4	2750	-	1161	16.4
100584	550	16.4	2750	-	1188	16.4
100585	550	16.4	2750	-	1215	16.4
100586	550	16.4	2750	-	1242	16.4
100587	550	16.4	2750	-	1269	16.4
100588	550	16.4	2750	-	1296	16.4
100589	550	16.4	2750	-	1323	16.4
100590	550	16.4	2750	-	1350	16.4
100591	550	16.4	2750	-	1377	16.4
100592	550	16.4	2750	-	1404	16.4
100593	550	16.4	2750	-	1431	16.4
100594	550	16.4	2750	-	1458	16.4
100595	550	16.4	2750	-	1485	16.4
100596	550	16.4	2750	-	1512	16.4
100633	500	15.5	2500	-	500	15.5
100634	500	15.5	2500	-	525	15.5
100635	500	15.5	2500	-	550	15.5
100636	500	15.5	2500	-	575	15.5
100637	500	15.5	2500	-	600	15.5
100638	500	15.5	2500	-	625	15.5
100639	500	15.5	2500	-	650	15.5
100640	500	15.1	2500	-	675	15.1
100641	500	15.1	2500	-	700	15.1
100642	500	15.1	2500	-	725	15.1
100643	500	15.1	2500	-	750	15.1
100644	500	15.1	2500	-	775	15.1
100645	500	15.1	2500	-	800	15.1
100646	500	15.1	2500	-	825	15.1
100647	500	15.1	2500	-	850	15.1
100648	500	15.1	2500	-	875	15.1
100649	500	15.1	2500	-	900	15.1
100650	500	15.1	2500	-	925	15.1
100651	500	15.1	2500	-	950	15.1
100652	500	15.1	2500	-	975	15.1
100653	500	15.1	2500	-	1000	15.1
100654	500	15.1	2500	-	1025	15.1
100655	500	15.1	2500	-	1050	15.1
100656	500	15.1	2500	-	1075	15.1
100657	500	14.9	2500	-	1100	14.9
100658	500	14.9	2500	-	1125	14.9
100659	500	14.9	2500	-	1150	14.9
100660	500	14.9	2500	-	1175	14.9
100661	500	14.9	2500	-	1200	14.9
100662	500	14.9	2500	-	1225	14.9
100663	500	14.9	2500	-	1250	14.9
100664	500	14.9	2500	-	1275	14.9
100665	500	14.9	2500	-	1300	14.9
100666	500	14.9	2500	-	1325	14.9
100667	500	14.9	2500	-	1350	14.9
100668	500	14.9	2500	-	1375	14.9
100669	500	14.9	2500	-	1400	14.9
100670	500	14.9	2500	-	1425	14.9
100671	500	14.9	2500	-	1450	14.9
100672	500	14.9	2500	-	1475	14.9
100673	500	14.9	2500	-	1500	14.9
100674	500	14.9	2500	-	1525	14.9
100433	650	20.1	3250	2.9	480	14.2
100558	550	17.1	2750	2.4	486	12.4
100492	600	18.5	3000	3.2	480	12.2
100143	1000	19.5	5000	4.9	450	9.7

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
100064	1150	25.3	5750	3.7	456	17.9
100246	850	21.6	4250	4.4	462	12.9
100632	500	14.8	2500	4.8	475	5.2

Overall score = score_ratio - 2 * repetitive DNA content