Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign111 - back to list

Summary
Image
Unique putative probes (234)
Putative probes (243)
Redundant probes (99)

Summary

`./analyse_probe_search_results : Fri Feb 17 09:58:35 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign111
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 14
  id            : 107
  strand        : 1
  -------------

Design window length: 3001bp

All jobs for probe design are successful - total: 46
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 907 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 907/907
Putative probes that are unique                 : 234/907
Putative probes exceeding selection criteria    : 576/907 (both unique and not unique)
Putative probes with successfully-picked primers: 499/576

Identified 99 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
101391	700	3500	-	1295
101420	650	3250	-	0
101421	650	3250	-	32
101461	650	3250	-	1312
101462	650	3250	-	1344
101494	600	3000	-	0
101495	600	3000	-	30
101496	600	3000	-	60
101537	600	3000	-	1290
101538	600	3000	-	1320
101539	600	3000	-	1350
101540	600	3000	-	1380
101575	550	2750	-	0
101576	550	2750	-	27
101577	550	2750	-	54
101578	550	2750	-	81
101579	550	2750	-	108
101580	550	2750	-	135
101623	550	2750	-	1296
101624	550	2750	-	1323
101625	550	2750	-	1350
101626	550	2750	-	1377
101627	550	2750	-	1404
101628	550	2750	-	1431
101666	500	2500	-	0
101667	500	2500	-	25
101668	500	2500	-	50
101669	500	2500	-	75
101670	500	2500	-	100
101671	500	2500	-	125
101672	500	2500	-	150
101673	500	2500	-	175
101718	500	2500	-	1300
101719	500	2500	-	1325
101720	500	2500	-	1350
101721	500	2500	-	1375
101722	500	2500	-	1400
101723	500	2500	-	1425
101724	500	2500	-	1450
101725	500	2500	-	1475
101726	500	2500	-	1500
101629	550	2750	0.5	1458
101541	600	3000	0.8	1410
101270	800	4000	1.0	1320
101328	750	3750	1.1	1295
101329	750	3750	1.1	1332
101330	750	3750	1.1	1369
101392	700	3500	1.1	1330
101393	700	3500	1.1	1365
101394	700	3500	1.1	1400
101463	650	3250	1.2	1376
101464	650	3250	1.2	1408
101465	650	3250	1.2	1440
101466	650	3250	1.2	1472
100880	1300	6500	1.3	1300
101542	600	3000	1.3	1440
101543	600	3000	1.3	1470
101544	600	3000	1.3	1500
100908	1250	6250	1.4	1302
100909	1250	6250	1.4	1364
100938	1200	6000	1.4	1320
100939	1200	6000	1.4	1380
101630	550	2750	1.5	1485
101631	550	2750	1.5	1512
101632	550	2750	1.5	1539
101633	550	2750	1.5	1566
100970	1150	5750	1.5	1311
100971	1150	5750	1.5	1368
100972	1150	5750	1.5	1425
100973	1150	5750	1.5	1482
101545	600	3000	1.5	1530
101004	1100	5500	1.5	1320
101005	1100	5500	1.5	1375
101006	1100	5500	1.5	1430
101007	1100	5500	1.5	1485
101727	500	2500	1.6	1525
101728	500	2500	1.6	1550
101729	500	2500	1.6	1575
101730	500	2500	1.6	1600
101731	500	2500	1.6	1625
101040	1050	5250	1.6	1300
101041	1050	5250	1.6	1352
101042	1050	5250	1.6	1404
101043	1050	5250	1.6	1456
101044	1050	5250	1.6	1508
101045	1050	5250	1.6	1560
101650	550	2750	1.6	2025
101651	550	2750	1.6	2052
101652	550	2750	1.6	2079
101079	1000	5000	1.7	1300
101080	1000	5000	1.7	1350
101081	1000	5000	1.7	1400
101082	1000	5000	1.7	1450
101083	1000	5000	1.7	1500
101084	1000	5000	1.7	1550
101085	1000	5000	1.7	1600
101122	950	4750	1.8	1316
101123	950	4750	1.8	1363
101124	950	4750	1.8	1410
101125	950	4750	1.8	1457
101126	950	4750	1.8	1504
101127	950	4750	1.8	1551
101128	950	4750	1.8	1598
101129	950	4750	1.8	1645
101747	500	2500	1.8	2025
101748	500	2500	1.8	2050
101749	500	2500	1.8	2075
101750	500	2500	1.8	2100
101751	500	2500	1.8	2125
101167	900	4500	1.9	1305
101168	900	4500	1.9	1350
101169	900	4500	1.9	1395
101170	900	4500	1.9	1440
101171	900	4500	1.9	1485
101172	900	4500	1.9	1530
101173	900	4500	1.9	1575
101174	900	4500	1.9	1620
101175	900	4500	1.9	1665
101176	900	4500	1.9	1710
101216	850	4250	2.0	1302
101217	850	4250	2.0	1344
101218	850	4250	2.0	1386
101219	850	4250	2.0	1428
101220	850	4250	2.0	1470
101221	850	4250	2.0	1512
101222	850	4250	2.0	1554
101223	850	4250	2.0	1596
101224	850	4250	2.0	1638
101225	850	4250	2.0	1680
101226	850	4250	2.0	1722
101227	850	4250	2.0	1764
101395	700	3500	2.0	1435
101561	600	3000	2.0	2010
101271	800	4000	2.1	1360
101272	800	4000	2.1	1400
101273	800	4000	2.1	1440
101274	800	4000	2.1	1480
101275	800	4000	2.1	1520
101276	800	4000	2.1	1560
101277	800	4000	2.1	1600
101278	800	4000	2.1	1640
101279	800	4000	2.1	1680
101280	800	4000	2.1	1720
101281	800	4000	2.1	1760
101282	800	4000	2.1	1800
101331	750	3750	2.3	1406
101332	750	3750	2.3	1443
101333	750	3750	2.3	1480
101334	750	3750	2.3	1517
101335	750	3750	2.3	1554
101336	750	3750	2.3	1591
101337	750	3750	2.3	1628
101338	750	3750	2.3	1665
101339	750	3750	2.3	1702
101340	750	3750	2.3	1739
101341	750	3750	2.3	1776
101342	750	3750	2.3	1813
101343	750	3750	2.3	1850
101396	700	3500	2.4	1470
101397	700	3500	2.4	1505
101398	700	3500	2.4	1540
101399	700	3500	2.4	1575
101400	700	3500	2.4	1610
101401	700	3500	2.4	1645
101402	700	3500	2.4	1680
101403	700	3500	2.4	1715
101404	700	3500	2.4	1750
101405	700	3500	2.4	1785
101406	700	3500	2.4	1820
101407	700	3500	2.4	1855
101408	700	3500	2.4	1890
101409	700	3500	2.4	1925
101467	650	3250	2.6	1504
101468	650	3250	2.6	1536
101469	650	3250	2.6	1568
101470	650	3250	2.6	1600
101471	650	3250	2.6	1632
101472	650	3250	2.6	1664
101473	650	3250	2.6	1696
101474	650	3250	2.6	1728
101475	650	3250	2.6	1760
101476	650	3250	2.6	1792
101477	650	3250	2.6	1824
101478	650	3250	2.6	1856
101479	650	3250	2.6	1888
101480	650	3250	2.6	1920
101481	650	3250	2.6	1952
101482	650	3250	2.6	1984
101546	600	3000	2.8	1560
101547	600	3000	2.8	1590
101548	600	3000	2.8	1620
101549	600	3000	2.8	1650
101550	600	3000	2.8	1680
101551	600	3000	2.8	1710
101552	600	3000	2.8	1740
101553	600	3000	2.8	1770
101554	600	3000	2.8	1800
101555	600	3000	2.8	1830
101556	600	3000	2.8	1860
101557	600	3000	2.8	1890
101558	600	3000	2.8	1920
101559	600	3000	2.8	1950
101560	600	3000	2.8	1980
101634	550	2750	3.1	1593
101635	550	2750	3.1	1620
101636	550	2750	3.1	1647
101637	550	2750	3.1	1674
101638	550	2750	3.1	1701
101639	550	2750	3.1	1728
101640	550	2750	3.1	1755
101641	550	2750	3.1	1782
101642	550	2750	3.1	1809
101643	550	2750	3.1	1836
101644	550	2750	3.1	1863
101645	550	2750	3.1	1890
101646	550	2750	3.1	1917
101647	550	2750	3.1	1944
101648	550	2750	3.1	1971
101649	550	2750	3.1	1998
101732	500	2500	3.4	1650
101733	500	2500	3.4	1675
101734	500	2500	3.4	1700
101735	500	2500	3.4	1725
101736	500	2500	3.4	1750
101737	500	2500	3.4	1775
101738	500	2500	3.4	1800
101739	500	2500	3.4	1825
101740	500	2500	3.4	1850
101741	500	2500	3.4	1875
101742	500	2500	3.4	1900
101743	500	2500	3.4	1925
101744	500	2500	3.4	1950
101745	500	2500	3.4	1975
101746	500	2500	3.4	2000

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
100881	1300	43.3	6500	1.3	1365	40.7
100882	1300	43.3	6500	1.3	1430	40.7
100883	1300	43.3	6500	1.3	1495	40.7
100884	1300	42.5	6500	1.3	1560	39.9
100910	1250	41.7	6250	1.4	1426	38.9
100911	1250	41.7	6250	1.4	1488	38.9
100912	1250	41.7	6250	1.4	1550	38.9
100913	1250	40.8	6250	1.4	1612	38.1
100875	1300	39.6	6500	1.3	975	37.0
100876	1300	39.6	6500	1.3	1040	37.0
100877	1300	39.6	6500	1.3	1105	37.0
100878	1300	39.6	6500	1.3	1170	37.0
100879	1300	39.6	6500	1.3	1235	37.0
100885	1300	39.4	6500	1.3	1625	36.8
100902	1250	38.1	6250	1.4	930	35.2
100874	1300	39.6	6500	2.9	910	33.8
100932	1200	36.6	6000	1.4	960	33.8
100964	1150	35.1	5750	0.7	969	33.7
100886	1300	35.3	6500	1.3	1690	32.7
100965	1150	35.1	5750	1.5	1026	32.1
100997	1100	33.5	5500	0.7	935	32.1
100998	1100	33.5	5500	0.7	990	32.1
101033	1050	32.0	5250	-	936	32.0
101034	1050	32.0	5250	0.8	988	30.5
101035	1050	32.0	5250	0.8	1040	30.5
101072	1000	30.5	5000	-	950	30.5
101073	1000	30.5	5000	-	1000	30.5
100999	1100	33.5	5500	1.5	1045	30.4
101000	1100	33.5	5500	1.5	1100	30.4
100963	1150	35.1	5750	2.3	912	30.4
100931	1200	36.6	6000	3.2	900	30.1
100946	1200	32.6	6000	1.4	1800	29.8
101114	950	29.0	4750	-	940	29.0
101115	950	29.0	4750	-	987	29.0
101116	950	29.0	4750	-	1034	29.0
101074	1000	30.5	5000	0.8	1050	28.9
101075	1000	30.5	5000	0.8	1100	28.9
101036	1050	32.0	5250	1.6	1092	28.8
101037	1050	32.0	5250	1.6	1144	28.8
101159	900	27.4	4500	-	945	27.4
101160	900	27.4	4500	-	990	27.4
101161	900	27.4	4500	-	1035	27.4
101162	900	27.4	4500	-	1080	27.4
101117	950	29.0	4750	0.8	1081	27.3
101118	950	29.0	4750	0.8	1128	27.3
101119	950	29.0	4750	0.8	1175	27.3
101076	1000	30.5	5000	1.7	1150	27.1
101077	1000	30.5	5000	1.7	1200	27.1
101208	850	25.9	4250	-	966	25.9
101209	850	25.9	4250	-	1008	25.9
101210	850	25.9	4250	-	1050	25.9
101211	850	25.9	4250	-	1092	25.9
101212	850	25.9	4250	-	1134	25.9
101237	800	26.0	4000	0.1	0	25.7
101163	900	27.4	4500	0.9	1125	25.7
101164	900	27.4	4500	0.9	1170	25.7
101165	900	27.4	4500	0.9	1215	25.7
101233	850	27.8	4250	1.1	2016	25.7
101120	950	29.0	4750	1.8	1222	25.4
101183	900	27.3	4500	1.0	2025	25.3
101293	750	24.5	3750	-	0	24.5
101294	750	24.5	3750	-	37	24.5
101288	800	26.7	4000	1.1	2040	24.4
101261	800	24.4	4000	-	960	24.4
101262	800	24.4	4000	-	1000	24.4
101263	800	24.4	4000	-	1040	24.4
101264	800	24.4	4000	-	1080	24.4
101265	800	24.4	4000	-	1120	24.4
101266	800	24.4	4000	-	1160	24.4
101267	800	24.4	4000	-	1200	24.4
101071	1000	30.5	5000	3.1	900	24.3
101207	850	25.9	4250	0.8	924	24.3
101213	850	25.9	4250	0.9	1176	24.0
101214	850	25.9	4250	0.9	1218	24.0
101215	850	25.9	4250	0.9	1260	24.0
101137	950	25.8	4750	0.9	2021	23.9
101289	800	26.1	4000	1.1	2080	23.9
101166	900	27.4	4500	1.9	1260	23.7
101234	850	25.8	4250	1.1	2058	23.6
101269	800	25.2	4000	1.0	1280	23.2
101236	850	23.1	4250	-	2142	23.1
101354	700	22.9	3500	-	0	22.9
101355	700	22.9	3500	-	35	22.9
101356	700	22.9	3500	-	70	22.9
101416	700	22.9	3500	-	2170	22.9
101319	750	22.9	3750	-	962	22.9
101320	750	22.9	3750	-	999	22.9
101321	750	22.9	3750	-	1036	22.9
101322	750	22.9	3750	-	1073	22.9
101323	750	22.9	3750	-	1110	22.9
101324	750	22.9	3750	-	1147	22.9
101325	750	22.9	3750	-	1184	22.9
101326	750	22.9	3750	-	1221	22.9
101351	750	22.7	3750	-	2146	22.7
101348	750	25.0	3750	1.2	2035	22.6
101349	750	25.0	3750	1.2	2072	22.6
101415	700	23.3	3500	0.4	2135	22.5
101184	900	24.5	4500	1.0	2070	22.5
101268	800	24.4	4000	1.0	1240	22.4
101352	750	22.3	3750	-	2183	22.3
101350	750	24.5	3750	1.2	2109	22.1
101327	750	22.9	3750	0.4	1258	22.1
101290	800	24.2	4000	1.1	2120	22.0
101291	800	21.7	4000	-	2160	21.7
101292	800	21.7	4000	-	2200	21.7
101487	650	21.7	3250	-	2144	21.7
101488	650	21.7	3250	-	2176	21.7
101260	800	24.4	4000	1.4	920	21.6
101381	700	21.3	3500	-	945	21.3
101382	700	21.3	3500	-	980	21.3
101383	700	21.3	3500	-	1015	21.3
101384	700	21.3	3500	-	1050	21.3
101385	700	21.3	3500	-	1085	21.3
101386	700	21.3	3500	-	1120	21.3
101387	700	21.3	3500	-	1155	21.3
101388	700	21.3	3500	-	1190	21.3
101389	700	21.3	3500	-	1225	21.3
101390	700	21.3	3500	-	1260	21.3
101318	750	22.9	3750	0.8	925	21.3
101422	650	21.2	3250	-	64	21.2
101423	650	21.2	3250	-	96	21.2
101424	650	21.2	3250	-	128	21.2
101489	650	21.2	3250	-	2208	21.2
101417	700	21.2	3500	-	2205	21.2
101357	700	22.7	3500	0.9	105	21.0
101235	850	23.1	4250	1.1	2100	21.0
101113	950	29.0	4750	4.0	893	21.0
101412	700	23.3	3500	1.3	2030	20.8
101413	700	23.3	3500	1.3	2065	20.8
101414	700	23.3	3500	1.3	2100	20.8
101460	650	20.4	3250	-	1280	20.4
101353	750	20.4	3750	-	2220	20.4
100996	1100	29.4	5500	4.6	880	20.1
101566	600	20.0	3000	-	2160	20.0
101567	600	20.0	3000	-	2190	20.0
101568	600	20.0	3000	-	2220	20.0
101569	600	20.0	3000	-	2250	20.0
101450	650	19.8	3250	-	960	19.8
101451	650	19.8	3250	-	992	19.8
101452	650	19.8	3250	-	1024	19.8
101453	650	19.8	3250	-	1056	19.8
101454	650	19.8	3250	-	1088	19.8
101455	650	19.8	3250	-	1120	19.8
101456	650	19.8	3250	-	1152	19.8
101457	650	19.8	3250	-	1184	19.8
101458	650	19.8	3250	-	1216	19.8
101459	650	19.8	3250	-	1248	19.8
101490	650	19.7	3250	-	2240	19.7
101491	650	19.7	3250	-	2272	19.7
101497	600	19.6	3000	-	90	19.6
101498	600	19.6	3000	-	120	19.6
101499	600	19.6	3000	-	150	19.6
101500	600	19.6	3000	-	180	19.6
101570	600	19.6	3000	-	2280	19.6
101418	700	19.3	3500	-	2240	19.3
101032	1050	28.2	5250	4.5	884	19.3
101419	700	19.0	3500	-	2275	19.0
101449	650	19.8	3250	0.5	928	18.9
101655	550	18.3	2750	-	2160	18.3
101656	550	18.3	2750	-	2187	18.3
101657	550	18.3	2750	-	2214	18.3
101658	550	18.3	2750	-	2241	18.3
101659	550	18.3	2750	-	2268	18.3
101660	550	18.3	2750	-	2295	18.3
101526	600	18.3	3000	-	960	18.3
101527	600	18.3	3000	-	990	18.3
101528	600	18.3	3000	-	1020	18.3
101529	600	18.3	3000	-	1050	18.3
101530	600	18.3	3000	-	1080	18.3
101531	600	18.3	3000	-	1110	18.3
101532	600	18.3	3000	-	1140	18.3
101533	600	18.3	3000	-	1170	18.3
101534	600	18.3	3000	-	1200	18.3
101535	600	18.3	3000	-	1230	18.3
101536	600	18.3	3000	-	1260	18.3
101571	600	18.2	3000	-	2310	18.2
101581	550	18.0	2750	-	162	18.0
101582	550	18.0	2750	-	189	18.0
101583	550	18.0	2750	-	216	18.0
101584	550	18.0	2750	-	243	18.0
101661	550	18.0	2750	-	2322	18.0
101525	600	18.3	3000	0.2	930	18.0
101425	650	21.1	3250	1.7	160	17.7
101295	750	24.4	3750	3.3	74	17.7
101492	650	17.7	3250	-	2304	17.7
101493	650	17.7	3250	-	2336	17.7
101610	550	16.8	2750	-	945	16.8
101611	550	16.8	2750	-	972	16.8
101612	550	16.8	2750	-	999	16.8
101613	550	16.8	2750	-	1026	16.8
101614	550	16.8	2750	-	1053	16.8
101615	550	16.8	2750	-	1080	16.8
101616	550	16.8	2750	-	1107	16.8
101617	550	16.8	2750	-	1134	16.8
101618	550	16.8	2750	-	1161	16.8
101619	550	16.8	2750	-	1188	16.8
101620	550	16.8	2750	-	1215	16.8
101621	550	16.8	2750	-	1242	16.8
101622	550	16.8	2750	-	1269	16.8
101752	500	16.7	2500	-	2150	16.7
101753	500	16.7	2500	-	2175	16.7
101754	500	16.7	2500	-	2200	16.7
101755	500	16.7	2500	-	2225	16.7
101756	500	16.7	2500	-	2250	16.7
101757	500	16.7	2500	-	2275	16.7
101758	500	16.7	2500	-	2300	16.7
101759	500	16.7	2500	-	2325	16.7
101662	550	16.7	2750	-	2349	16.7
101760	500	16.7	2500	-	2350	16.7
101663	550	16.7	2750	-	2376	16.7
101572	600	16.6	3000	-	2340	16.6
101654	550	18.3	2750	0.9	2133	16.5
101674	500	16.3	2500	-	200	16.3
101675	500	16.3	2500	-	225	16.3
101676	500	16.3	2500	-	250	16.3
101677	500	16.3	2500	-	275	16.3
101761	500	16.3	2500	-	2375	16.3
101573	600	16.3	3000	-	2370	16.3
101574	600	16.3	3000	-	2400	16.3
101501	600	19.5	3000	1.8	210	15.8
101704	500	15.2	2500	-	950	15.2
101705	500	15.2	2500	-	975	15.2
101706	500	15.2	2500	-	1000	15.2
101707	500	15.2	2500	-	1025	15.2
101708	500	15.2	2500	-	1050	15.2
101709	500	15.2	2500	-	1075	15.2
101710	500	15.2	2500	-	1100	15.2
101711	500	15.2	2500	-	1125	15.2
101712	500	15.2	2500	-	1150	15.2
101713	500	15.2	2500	-	1175	15.2
101714	500	15.2	2500	-	1200	15.2
101715	500	15.2	2500	-	1225	15.2
101716	500	15.2	2500	-	1250	15.2
101717	500	15.2	2500	-	1275	15.2
101762	500	15.2	2500	-	2400	15.2
101763	500	15.2	2500	-	2425	15.2
101664	550	14.9	2750	-	2403	14.9
101665	550	14.9	2750	-	2430	14.9
101764	500	13.6	2500	-	2450	13.6
101765	500	13.6	2500	-	2475	13.6
101766	500	13.6	2500	-	2500	13.6
101703	500	15.2	2500	1.2	925	12.8
101609	550	16.8	2750	2.4	918	12.0

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
100940	1200	40.0	6000	1.4	1440	37.2
100941	1200	40.0	6000	1.4	1500	37.2
100942	1200	40.0	6000	1.4	1560	37.2
100943	1200	40.0	6000	1.4	1620	37.2
100944	1200	39.2	6000	1.4	1680	36.4
100903	1250	38.1	6250	1.4	992	35.4
100904	1250	38.1	6250	1.4	1054	35.4
100905	1250	38.1	6250	1.4	1116	35.4
100906	1250	38.1	6250	1.4	1178	35.4
100907	1250	38.1	6250	1.4	1240	35.4
100974	1150	38.3	5750	1.5	1539	35.4
100975	1150	38.3	5750	1.5	1596	35.4
100976	1150	38.3	5750	1.5	1653	35.4
100914	1250	37.9	6250	1.4	1674	35.2
100977	1150	37.6	5750	1.5	1710	34.6
100933	1200	36.6	6000	1.4	1020	33.8
100934	1200	36.6	6000	1.4	1080	33.8
100935	1200	36.6	6000	1.4	1140	33.8
100936	1200	36.6	6000	1.4	1200	33.8
100937	1200	36.6	6000	1.4	1260	33.8
101008	1100	36.7	5500	1.5	1540	33.6
101009	1100	36.7	5500	1.5	1595	33.6
101010	1100	36.7	5500	1.5	1650	33.6
101011	1100	36.7	5500	1.5	1705	33.6
101012	1100	35.9	5500	1.5	1760	32.9
100966	1150	35.1	5750	1.5	1083	32.1
100967	1150	35.1	5750	1.5	1140	32.1
100968	1150	35.1	5750	1.5	1197	32.1
100969	1150	35.1	5750	1.5	1254	32.1
100978	1150	34.8	5750	1.5	1767	31.9
101046	1050	35.0	5250	1.6	1612	31.8
101047	1050	35.0	5250	1.6	1664	31.8
101048	1050	35.0	5250	1.6	1716	31.8
101049	1050	35.0	5250	1.6	1768	31.8
100915	1250	34.0	6250	1.4	1736	31.2
101050	1050	34.3	5250	1.6	1820	31.1
101001	1100	33.5	5500	1.5	1155	30.4
101002	1100	33.5	5500	1.5	1210	30.4
101003	1100	33.5	5500	1.5	1265	30.4
100945	1200	33.1	6000	1.4	1740	30.3
101013	1100	33.3	5500	1.5	1815	30.2
101086	1000	33.3	5000	1.7	1650	29.9
101087	1000	33.3	5000	1.7	1700	29.9
101088	1000	33.3	5000	1.7	1750	29.9
101089	1000	33.3	5000	1.7	1800	29.9
101090	1000	33.3	5000	1.7	1850	29.9
101038	1050	32.0	5250	1.6	1196	28.8
101039	1050	32.0	5250	1.6	1248	28.8
101051	1050	31.8	5250	1.6	1872	28.6
100979	1150	31.2	5750	1.5	1824	28.3
101130	950	31.7	4750	1.8	1692	28.1
101131	950	31.7	4750	1.8	1739	28.1
101132	950	31.7	4750	1.8	1786	28.1
101133	950	31.7	4750	1.8	1833	28.1
101134	950	31.7	4750	1.8	1880	28.1
101135	950	31.0	4750	1.8	1927	27.5
101078	1000	30.5	5000	1.7	1250	27.1
101091	1000	30.3	5000	1.7	1900	26.9
101014	1100	29.9	5500	1.5	1870	26.8
101177	900	30.0	4500	1.9	1755	26.2
101178	900	30.0	4500	1.9	1800	26.2
101179	900	30.0	4500	1.9	1845	26.2
101180	900	30.0	4500	1.9	1890	26.2
101181	900	30.0	4500	1.9	1935	26.2
101182	900	29.4	4500	1.9	1980	25.6
101121	950	29.0	4750	1.8	1269	25.4
101052	1050	28.5	5250	1.6	1924	25.3
101136	950	28.8	4750	1.8	1974	25.2
101228	850	28.3	4250	2.0	1806	24.3
101229	850	28.3	4250	2.0	1848	24.3
101230	850	28.3	4250	2.0	1890	24.3
101231	850	28.3	4250	2.0	1932	24.3
101232	850	28.3	4250	2.0	1974	24.3
101092	1000	27.2	5000	1.7	1950	23.8
101093	1000	27.2	5000	1.7	2000	23.8
101283	800	26.7	4000	2.1	1840	22.4
101284	800	26.7	4000	2.1	1880	22.4
101285	800	26.7	4000	2.1	1920	22.4
101286	800	26.7	4000	2.1	1960	22.4
101287	800	26.7	4000	2.1	2000	22.4
101158	900	27.4	4500	3.4	900	20.6
101344	750	25.0	3750	2.3	1887	20.5
101345	750	25.0	3750	2.3	1924	20.5
101346	750	25.0	3750	2.3	1961	20.5
101347	750	25.0	3750	2.3	1998	20.5
101483	650	21.7	3250	1.4	2016	18.9
101484	650	21.7	3250	1.4	2048	18.9
101485	650	21.7	3250	1.4	2080	18.9
101486	650	21.7	3250	1.4	2112	18.9
101410	700	23.3	3500	2.4	1960	18.5
101411	700	23.3	3500	2.4	1995	18.5
101565	600	20.0	3000	1.3	2130	17.3
101562	600	20.0	3000	1.5	2040	17.0
101563	600	20.0	3000	1.5	2070	17.0
101564	600	20.0	3000	1.5	2100	17.0
101678	500	16.2	2500	0.2	300	15.8
101380	700	21.3	3500	3.0	910	15.3
101653	550	18.3	2750	1.6	2106	15.1
101585	550	17.9	2750	3.8	270	10.2

Overall score = score_ratio - 2 * repetitive DNA content