Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign116 - back to list

Summary
Image
Unique putative probes (228)
Putative probes (469)
Redundant probes (6)

Summary

`./analyse_probe_search_results : Wed Jun 28 14:26:23 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign116
  assembly      : NCBIM36
  bias          : 5prime
  chromosome    : 7
  id            : 119
  strand        : 1
  -------------

Design window length: 3501bp

All jobs for probe design are successful - total: 56
Fetched conf : Exonerate mouse_NCBIM36 (id: 2)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 1114 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1114/1114
Putative probes that are unique                 : 228/1114
Putative probes exceeding selection criteria    : 703/1114 (both unique and not unique)
Putative probes with successfully-picked primers: 443/703

Identified 6 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
111227	1300	6500	-	1495
111262	1250	6250	-	1488
111263	1250	6250	-	1550
111300	1200	6000	-	1500
111301	1200	6000	-	1560
111302	1200	6000	-	1620
111341	1150	5750	-	1539
111342	1150	5750	-	1596
111343	1150	5750	-	1653
111384	1100	5500	-	1540
111385	1100	5500	-	1595
111386	1100	5500	-	1650
111387	1100	5500	-	1705
111429	1050	5250	-	1508
111430	1050	5250	-	1560
111431	1050	5250	-	1612
111432	1050	5250	-	1664
111433	1050	5250	-	1716
111434	1050	5250	-	1768
111478	1000	5000	-	1500
111479	1000	5000	-	1550
111480	1000	5000	-	1600
111481	1000	5000	-	1650
111482	1000	5000	-	1700
111483	1000	5000	-	1750
111484	1000	5000	-	1800
111531	950	4750	-	1504
111532	950	4750	-	1551
111533	950	4750	-	1598
111534	950	4750	-	1645
111535	950	4750	-	1692
111536	950	4750	-	1739
111537	950	4750	-	1786
111538	950	4750	-	1833
111539	950	4750	-	1880
111588	900	4500	-	1530
111589	900	4500	-	1575
111590	900	4500	-	1620
111591	900	4500	-	1665
111592	900	4500	-	1710
111593	900	4500	-	1755
111594	900	4500	-	1800
111595	900	4500	-	1845
111596	900	4500	-	1890
111597	900	4500	-	1935
111648	850	4250	-	1512
111649	850	4250	-	1554
111650	850	4250	-	1596
111651	850	4250	-	1638
111652	850	4250	-	1680
111653	850	4250	-	1722
111654	850	4250	-	1764
111655	850	4250	-	1806
111656	850	4250	-	1848
111657	850	4250	-	1890
111658	850	4250	-	1932
111659	850	4250	-	1974
111714	800	4000	-	1520
111715	800	4000	-	1560
111716	800	4000	-	1600
111717	800	4000	-	1640
111718	800	4000	-	1680
111719	800	4000	-	1720
111720	800	4000	-	1760
111721	800	4000	-	1800
111722	800	4000	-	1840
111723	800	4000	-	1880
111724	800	4000	-	1920
111725	800	4000	-	1960
111726	800	4000	-	2000
111727	800	4000	-	2040
111785	750	3750	-	1517
111786	750	3750	-	1554
111787	750	3750	-	1591
111788	750	3750	-	1628
111789	750	3750	-	1665
111790	750	3750	-	1702
111791	750	3750	-	1739
111792	750	3750	-	1776
111793	750	3750	-	1813
111794	750	3750	-	1850
111795	750	3750	-	1887
111796	750	3750	-	1924
111797	750	3750	-	1961
111798	750	3750	-	1998
111799	750	3750	-	2035
111800	750	3750	-	2072
111862	700	3500	-	1505
111863	700	3500	-	1540
111864	700	3500	-	1575
111865	700	3500	-	1610
111866	700	3500	-	1645
111867	700	3500	-	1680
111868	700	3500	-	1715
111869	700	3500	-	1750
111870	700	3500	-	1785
111871	700	3500	-	1820
111872	700	3500	-	1855
111873	700	3500	-	1890
111874	700	3500	-	1925
111875	700	3500	-	1960
111876	700	3500	-	1995
111877	700	3500	-	2030
111878	700	3500	-	2065
111879	700	3500	-	2100
111880	700	3500	-	2135
111947	650	3250	-	1504
111948	650	3250	-	1536
111949	650	3250	-	1568
111950	650	3250	-	1600
111951	650	3250	-	1632
111952	650	3250	-	1664
111953	650	3250	-	1696
111954	650	3250	-	1728
111955	650	3250	-	1760
111956	650	3250	-	1792
111957	650	3250	-	1824
111958	650	3250	-	1856
111959	650	3250	-	1888
111960	650	3250	-	1920
111961	650	3250	-	1952
111962	650	3250	-	1984
111963	650	3250	-	2016
111964	650	3250	-	2048
111965	650	3250	-	2080
111966	650	3250	-	2112
111967	650	3250	-	2144
111968	650	3250	-	2176
111990	600	3000	-	0
111991	600	3000	-	30
112040	600	3000	-	1500
112041	600	3000	-	1530
112042	600	3000	-	1560
112043	600	3000	-	1590
112044	600	3000	-	1620
112045	600	3000	-	1650
112046	600	3000	-	1680
112047	600	3000	-	1710
112048	600	3000	-	1740
112049	600	3000	-	1770
112050	600	3000	-	1800
112051	600	3000	-	1830
112052	600	3000	-	1860
112053	600	3000	-	1890
112054	600	3000	-	1920
112055	600	3000	-	1950
112056	600	3000	-	1980
112057	600	3000	-	2010
112058	600	3000	-	2040
112059	600	3000	-	2070
112060	600	3000	-	2100
112061	600	3000	-	2130
112062	600	3000	-	2160
112063	600	3000	-	2190
112064	600	3000	-	2220
112087	550	2750	-	0
112088	550	2750	-	27
112089	550	2750	-	54
112090	550	2750	-	81
112143	550	2750	-	1512
112144	550	2750	-	1539
112145	550	2750	-	1566
112146	550	2750	-	1593
112147	550	2750	-	1620
112148	550	2750	-	1647
112149	550	2750	-	1674
112150	550	2750	-	1701
112151	550	2750	-	1728
112152	550	2750	-	1755
112153	550	2750	-	1782
112154	550	2750	-	1809
112155	550	2750	-	1836
112156	550	2750	-	1863
112157	550	2750	-	1890
112158	550	2750	-	1917
112159	550	2750	-	1944
112160	550	2750	-	1971
112161	550	2750	-	1998
112162	550	2750	-	2025
112163	550	2750	-	2052
112164	550	2750	-	2079
112165	550	2750	-	2106
112166	550	2750	-	2133
112167	550	2750	-	2160
112168	550	2750	-	2187
112169	550	2750	-	2214
112170	550	2750	-	2241
112171	550	2750	-	2268
112197	500	2500	-	0
112198	500	2500	-	25
112199	500	2500	-	50
112200	500	2500	-	75
112201	500	2500	-	100
112202	500	2500	-	125
112257	500	2500	-	1500
112258	500	2500	-	1525
112259	500	2500	-	1550
112260	500	2500	-	1575
112261	500	2500	-	1600
112262	500	2500	-	1625
112263	500	2500	-	1650
112264	500	2500	-	1675
112265	500	2500	-	1700
112266	500	2500	-	1725
112267	500	2500	-	1750
112268	500	2500	-	1775
112269	500	2500	-	1800
112270	500	2500	-	1825
112271	500	2500	-	1850
112272	500	2500	-	1875
112273	500	2500	-	1900
112274	500	2500	-	1925
112275	500	2500	-	1950
112276	500	2500	-	1975
112277	500	2500	-	2000
112278	500	2500	-	2025
112279	500	2500	-	2050
112280	500	2500	-	2075
112281	500	2500	-	2100
112282	500	2500	-	2125
112283	500	2500	-	2150
112284	500	2500	-	2175
112285	500	2500	-	2200
112286	500	2500	-	2225
112287	500	2500	-	2250
112288	500	2500	-	2275
112289	500	2500	-	2300
112290	500	2500	-	2325

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
111228	1300	42.2	6500	-	1560	42.2
111229	1300	42.2	6500	-	1625	42.2
111230	1300	42.2	6500	-	1690	42.2
111231	1300	41.1	6500	-	1755	41.1
111264	1250	40.6	6250	-	1612	40.6
111265	1250	40.6	6250	-	1674	40.6
111266	1250	40.6	6250	-	1736	40.6
111232	1300	40.1	6500	-	1820	40.1
111233	1300	40.1	6500	-	1885	40.1
111234	1300	40.1	6500	-	1950	40.1
111267	1250	39.6	6250	-	1798	39.6
111303	1200	39.0	6000	-	1680	39.0
111304	1200	39.0	6000	-	1740	39.0
111305	1200	39.0	6000	-	1800	39.0
111268	1250	38.6	6250	-	1860	38.6
111269	1250	38.6	6250	-	1922	38.6
111270	1250	38.6	6250	-	1984	38.6
111306	1200	38.0	6000	-	1860	38.0
111344	1150	37.3	5750	-	1710	37.3
111345	1150	37.3	5750	-	1767	37.3
111346	1150	37.3	5750	-	1824	37.3
111347	1150	37.3	5750	-	1881	37.3
111307	1200	37.0	6000	-	1920	37.0
111308	1200	37.0	6000	-	1980	37.0
111309	1200	37.0	6000	-	2040	37.0
111388	1100	35.7	5500	-	1760	35.7
111389	1100	35.7	5500	-	1815	35.7
111390	1100	35.7	5500	-	1870	35.7
111391	1100	35.7	5500	-	1925	35.7
111348	1150	35.5	5750	-	1938	35.5
111349	1150	35.5	5750	-	1995	35.5
111350	1150	35.5	5750	-	2052	35.5
111351	1150	35.5	5750	-	2109	35.5
111271	1250	38.6	6250	2.2	2046	34.1
111435	1050	34.1	5250	-	1820	34.1
111436	1050	34.1	5250	-	1872	34.1
111437	1050	34.1	5250	-	1924	34.1
111438	1050	34.1	5250	-	1976	34.1
111383	1100	34.0	5500	-	1485	34.0
111392	1100	34.0	5500	-	1980	34.0
111393	1100	34.0	5500	-	2035	34.0
111394	1100	34.0	5500	-	2090	34.0
111395	1100	34.0	5500	-	2145	34.0
111340	1150	32.5	5750	-	1482	32.5
111485	1000	32.5	5000	-	1850	32.5
111486	1000	32.5	5000	-	1900	32.5
111487	1000	32.5	5000	-	1950	32.5
111488	1000	32.5	5000	-	2000	32.5
111439	1050	32.4	5250	-	2028	32.4
111440	1050	32.4	5250	-	2080	32.4
111441	1050	32.4	5250	-	2132	32.4
111442	1050	32.4	5250	-	2184	32.4
111310	1200	37.0	6000	2.7	2100	31.7
111489	1000	31.6	5000	-	2050	31.6
111490	1000	30.9	5000	-	2100	30.9
111491	1000	30.9	5000	-	2150	30.9
111492	1000	30.9	5000	-	2200	30.9
111493	1000	30.9	5000	-	2250	30.9
111540	950	30.8	4750	-	1927	30.8
111541	950	30.8	4750	-	1974	30.8
111542	950	30.8	4750	-	2021	30.8
111543	950	30.8	4750	-	2068	30.8
111544	950	30.1	4750	-	2115	30.1
111235	1300	36.7	6500	3.6	2015	29.5
111545	950	29.3	4750	-	2162	29.3
111546	950	29.3	4750	-	2209	29.3
111547	950	29.3	4750	-	2256	29.3
111548	950	29.3	4750	-	2303	29.3
111598	900	29.2	4500	-	1980	29.2
111599	900	29.2	4500	-	2025	29.2
111600	900	29.2	4500	-	2070	29.2
111601	900	29.2	4500	-	2115	29.2
111443	1050	32.4	5250	1.7	2236	29.0
111602	900	28.5	4500	-	2160	28.5
111396	1100	34.0	5500	2.9	2200	28.1
111587	900	27.8	4500	-	1485	27.8
111603	900	27.8	4500	-	2205	27.8
111604	900	27.8	4500	-	2250	27.8
111605	900	27.8	4500	-	2295	27.8
111606	900	27.8	4500	-	2340	27.8
111660	850	27.6	4250	-	2016	27.6
111661	850	27.6	4250	-	2058	27.6
111662	850	27.6	4250	-	2100	27.6
111663	850	27.6	4250	-	2142	27.6
111664	850	26.9	4250	-	2184	26.9
111665	850	26.2	4250	-	2226	26.2
111666	850	26.2	4250	-	2268	26.2
111667	850	26.2	4250	-	2310	26.2
111668	850	26.2	4250	-	2352	26.2
111669	850	26.2	4250	-	2394	26.2
111236	1300	35.1	6500	4.5	2080	26.1
111237	1300	35.1	6500	4.5	2145	26.1
111728	800	26.0	4000	-	2080	26.0
111729	800	26.0	4000	-	2120	26.0
111730	800	26.0	4000	-	2160	26.0
111731	800	26.0	4000	-	2200	26.0
111220	1300	25.7	6500	-	1040	25.7
111221	1300	25.7	6500	-	1105	25.7
111222	1300	25.7	6500	-	1170	25.7
111223	1300	25.7	6500	-	1235	25.7
111224	1300	25.7	6500	-	1300	25.7
111225	1300	25.7	6500	-	1365	25.7
111226	1300	25.7	6500	-	1430	25.7
111732	800	25.3	4000	-	2240	25.3
111255	1250	24.7	6250	-	1054	24.7
111256	1250	24.7	6250	-	1116	24.7
111257	1250	24.7	6250	-	1178	24.7
111258	1250	24.7	6250	-	1240	24.7
111259	1250	24.7	6250	-	1302	24.7
111260	1250	24.7	6250	-	1364	24.7
111261	1250	24.7	6250	-	1426	24.7
111733	800	24.7	4000	-	2280	24.7
111734	800	24.7	4000	-	2320	24.7
111735	800	24.7	4000	-	2360	24.7
111736	800	24.7	4000	-	2400	24.7
111737	800	24.7	4000	-	2440	24.7
111494	1000	30.9	5000	3.2	2300	24.5
111801	750	24.4	3750	-	2109	24.4
111802	750	24.4	3750	-	2146	24.4
111803	750	24.4	3750	-	2183	24.4
111804	750	24.4	3750	-	2220	24.4
111805	750	24.4	3750	-	2257	24.4
111274	1250	33.8	6250	4.7	2232	24.3
111352	1150	32.5	5750	4.2	2166	24.1
111607	900	27.8	4500	1.9	2385	24.0
111806	750	23.7	3750	-	2294	23.7
111293	1200	23.7	6000	-	1080	23.7
111294	1200	23.7	6000	-	1140	23.7
111295	1200	23.7	6000	-	1200	23.7
111296	1200	23.7	6000	-	1260	23.7
111297	1200	23.7	6000	-	1320	23.7
111298	1200	23.7	6000	-	1380	23.7
111299	1200	23.7	6000	-	1440	23.7
111807	750	23.1	3750	-	2331	23.1
111808	750	23.1	3750	-	2368	23.1
111809	750	23.1	3750	-	2405	23.1
111810	750	23.1	3750	-	2442	23.1
111811	750	23.1	3750	-	2479	23.1
111812	750	23.1	3750	-	2516	23.1
111332	1150	22.7	5750	-	1026	22.7
111333	1150	22.7	5750	-	1083	22.7
111334	1150	22.7	5750	-	1140	22.7
111335	1150	22.7	5750	-	1197	22.7
111336	1150	22.7	5750	-	1254	22.7
111337	1150	22.7	5750	-	1311	22.7
111338	1150	22.7	5750	-	1368	22.7
111339	1150	22.7	5750	-	1425	22.7
111881	700	22.7	3500	-	2170	22.7
111882	700	22.7	3500	-	2205	22.7
111883	700	22.7	3500	-	2240	22.7
111884	700	22.7	3500	-	2275	22.7
111885	700	22.7	3500	-	2310	22.7
111549	950	29.3	4750	3.4	2350	22.6
111886	700	22.2	3500	-	2345	22.2
111670	850	26.2	4250	2.1	2436	22.0
111375	1100	21.7	5500	-	1045	21.7
111376	1100	21.7	5500	-	1100	21.7
111377	1100	21.7	5500	-	1155	21.7
111378	1100	21.7	5500	-	1210	21.7
111379	1100	21.7	5500	-	1265	21.7
111380	1100	21.7	5500	-	1320	21.7
111381	1100	21.7	5500	-	1375	21.7
111382	1100	21.7	5500	-	1430	21.7
111738	800	24.7	4000	1.5	2480	21.7
111900	650	21.7	3250	-	0	21.7
111887	700	21.6	3500	-	2380	21.6
111888	700	21.6	3500	-	2415	21.6
111889	700	21.6	3500	-	2450	21.6
111890	700	21.6	3500	-	2485	21.6
111891	700	21.6	3500	-	2520	21.6
111892	700	21.6	3500	-	2555	21.6
111292	1200	21.6	6000	-	1020	21.6
111713	800	21.4	4000	-	1480	21.4
111969	650	21.1	3250	-	2208	21.1
111970	650	21.1	3250	-	2240	21.1
111971	650	21.1	3250	-	2272	21.1
111972	650	21.1	3250	-	2304	21.1
111973	650	21.1	3250	-	2336	21.1
111974	650	21.1	3250	-	2368	21.1
111420	1050	20.8	5250	-	1040	20.8
111421	1050	20.8	5250	-	1092	20.8
111422	1050	20.8	5250	-	1144	20.8
111423	1050	20.8	5250	-	1196	20.8
111424	1050	20.8	5250	-	1248	20.8
111425	1050	20.8	5250	-	1300	20.8
111426	1050	20.8	5250	-	1352	20.8
111427	1050	20.8	5250	-	1404	20.8
111428	1050	20.8	5250	-	1456	20.8
111975	650	20.6	3250	-	2400	20.6
111819	700	20.5	3500	-	0	20.5
111976	650	20.1	3250	-	2432	20.1
111977	650	20.1	3250	-	2464	20.1
111978	650	20.1	3250	-	2496	20.1
111979	650	20.1	3250	-	2528	20.1
111980	650	20.1	3250	-	2560	20.1
111981	650	20.1	3250	-	2592	20.1
111784	750	20.1	3750	-	1480	20.1
111992	600	20.0	3000	-	60	20.0
111469	1000	19.8	5000	-	1050	19.8
111470	1000	19.8	5000	-	1100	19.8
111471	1000	19.8	5000	-	1150	19.8
111472	1000	19.8	5000	-	1200	19.8
111473	1000	19.8	5000	-	1250	19.8
111474	1000	19.8	5000	-	1300	19.8
111475	1000	19.8	5000	-	1350	19.8
111476	1000	19.8	5000	-	1400	19.8
111477	1000	19.8	5000	-	1450	19.8
111254	1250	19.6	6250	-	992	19.6
112065	600	19.5	3000	-	2250	19.5
112066	600	19.5	3000	-	2280	19.5
112067	600	19.5	3000	-	2310	19.5
112068	600	19.5	3000	-	2340	19.5
112069	600	19.5	3000	-	2370	19.5
112070	600	19.5	3000	-	2400	19.5
112071	600	19.5	3000	-	2430	19.5
111901	650	19.0	3250	-	32	19.0
112072	600	19.0	3000	-	2460	19.0
111521	950	18.8	4750	-	1034	18.8
111522	950	18.8	4750	-	1081	18.8
111523	950	18.8	4750	-	1128	18.8
111524	950	18.8	4750	-	1175	18.8
111525	950	18.8	4750	-	1222	18.8
111526	950	18.8	4750	-	1269	18.8
111527	950	18.8	4750	-	1316	18.8
111528	950	18.8	4750	-	1363	18.8
111529	950	18.8	4750	-	1410	18.8
111530	950	18.8	4750	-	1457	18.8
111219	1300	18.7	6500	-	975	18.7
111647	850	18.6	4250	-	1470	18.6
111218	1300	18.6	6500	-	910	18.6
112073	600	18.5	3000	-	2490	18.5
112074	600	18.5	3000	-	2520	18.5
112075	600	18.5	3000	-	2550	18.5
112076	600	18.5	3000	-	2580	18.5
112077	600	18.5	3000	-	2610	18.5
112078	600	18.5	3000	-	2640	18.5
111744	750	18.5	3750	-	0	18.5
112091	550	18.3	2750	-	108	18.3
111982	650	20.1	3250	0.9	2624	18.2
112172	550	18.1	2750	-	2295	18.1
111252	1250	17.9	6250	-	868	17.9
111253	1250	17.9	6250	-	930	17.9
111217	1300	17.9	6500	-	845	17.9
112173	550	17.9	2750	-	2322	17.9
112174	550	17.9	2750	-	2349	17.9
112175	550	17.9	2750	-	2376	17.9
112176	550	17.9	2750	-	2403	17.9
112177	550	17.9	2750	-	2430	17.9
112178	550	17.9	2750	-	2457	17.9
112079	600	18.5	3000	0.3	2670	17.9
111577	900	17.8	4500	-	1035	17.8
111578	900	17.8	4500	-	1080	17.8
111579	900	17.8	4500	-	1125	17.8
111580	900	17.8	4500	-	1170	17.8
111581	900	17.8	4500	-	1215	17.8
111582	900	17.8	4500	-	1260	17.8
111583	900	17.8	4500	-	1305	17.8
111584	900	17.8	4500	-	1350	17.8
111585	900	17.8	4500	-	1395	17.8
111586	900	17.8	4500	-	1440	17.8
111902	650	17.7	3250	-	64	17.7
111993	600	17.5	3000	-	90	17.5
112179	550	17.4	2750	-	2484	17.4
112180	550	17.4	2750	-	2511	17.4
111820	700	17.2	3500	-	35	17.2
111290	1200	17.2	6000	-	900	17.2
111291	1200	17.2	6000	-	960	17.2
111251	1250	17.2	6250	-	806	17.2
112142	550	17.0	2750	-	1485	17.0
112181	550	17.0	2750	-	2538	17.0
112182	550	17.0	2750	-	2565	17.0
112183	550	17.0	2750	-	2592	17.0
112184	550	17.0	2750	-	2619	17.0
112185	550	17.0	2750	-	2646	17.0
112186	550	17.0	2750	-	2673	17.0
112187	550	17.0	2750	-	2700	17.0
111637	850	16.8	4250	-	1050	16.8
111638	850	16.8	4250	-	1092	16.8
111639	850	16.8	4250	-	1134	16.8
111640	850	16.8	4250	-	1176	16.8
111641	850	16.8	4250	-	1218	16.8
111642	850	16.8	4250	-	1260	16.8
111643	850	16.8	4250	-	1302	16.8
111644	850	16.8	4250	-	1344	16.8
111645	850	16.8	4250	-	1386	16.8
111646	850	16.8	4250	-	1428	16.8
111374	1100	16.7	5500	-	990	16.7
112203	500	16.7	2500	-	150	16.7
111289	1200	16.5	6000	-	840	16.5
111330	1150	16.5	5750	-	912	16.5
111331	1150	16.5	5750	-	969	16.5
111468	1000	16.3	5000	-	1000	16.3
111821	700	16.3	3500	-	70	16.3
111329	1150	16.2	5750	-	855	16.2
112291	500	16.2	2500	-	2350	16.2
112292	500	16.2	2500	-	2375	16.2
112293	500	16.2	2500	-	2400	16.2
112294	500	16.2	2500	-	2425	16.2
112295	500	16.2	2500	-	2450	16.2
112296	500	16.2	2500	-	2475	16.2
112297	500	16.2	2500	-	2500	16.2
112298	500	16.2	2500	-	2525	16.2
112092	550	16.1	2750	-	135	16.1
111903	650	16.0	3250	-	96	16.0
112299	500	15.8	2500	-	2550	15.8
111702	800	15.8	4000	-	1040	15.8
111703	800	15.8	4000	-	1080	15.8
111704	800	15.8	4000	-	1120	15.8
111705	800	15.8	4000	-	1160	15.8
111706	800	15.8	4000	-	1200	15.8
111707	800	15.8	4000	-	1240	15.8
111708	800	15.8	4000	-	1280	15.8
111709	800	15.8	4000	-	1320	15.8
111710	800	15.8	4000	-	1360	15.8
111711	800	15.8	4000	-	1400	15.8
111712	800	15.8	4000	-	1440	15.8
111372	1100	15.8	5500	-	880	15.8
111373	1100	15.8	5500	-	935	15.8
112093	550	15.5	2750	-	162	15.5
111419	1050	15.5	5250	-	988	15.5
112300	500	15.4	2500	-	2575	15.4
112301	500	15.4	2500	-	2600	15.4
112302	500	15.4	2500	-	2625	15.4
112303	500	15.4	2500	-	2650	15.4
112304	500	15.4	2500	-	2675	15.4
112305	500	15.4	2500	-	2700	15.4
112306	500	15.4	2500	-	2725	15.4
112307	500	15.4	2500	-	2750	15.4
111861	700	15.4	3500	-	1470	15.4
111893	700	21.6	3500	3.1	2590	15.3
111371	1100	15.1	5500	-	825	15.1
111417	1050	15.0	5250	-	884	15.0
111418	1050	15.0	5250	-	936	15.0
111994	600	15.0	3000	-	120	15.0
111946	650	14.9	3250	-	1472	14.9
111772	750	14.8	3750	-	1036	14.8
111773	750	14.8	3750	-	1073	14.8
111774	750	14.8	3750	-	1110	14.8
111775	750	14.8	3750	-	1147	14.8
111776	750	14.8	3750	-	1184	14.8
111777	750	14.8	3750	-	1221	14.8
111778	750	14.8	3750	-	1258	14.8
111779	750	14.8	3750	-	1295	14.8
111780	750	14.8	3750	-	1332	14.8
111781	750	14.8	3750	-	1369	14.8
111782	750	14.8	3750	-	1406	14.8
111783	750	14.8	3750	-	1443	14.8
111995	600	14.8	3000	-	150	14.8
111745	750	14.6	3750	-	37	14.6
112204	500	14.6	2500	-	175	14.6
112205	500	14.6	2500	-	200	14.6
111636	850	14.5	4250	-	1008	14.5
111416	1050	14.4	5250	-	832	14.4
111676	800	14.4	4000	-	0	14.4
111466	1000	14.3	5000	-	900	14.3
111467	1000	14.3	5000	-	950	14.3
111465	1000	14.0	5000	-	850	14.0
111904	650	13.9	3250	-	128	13.9
111849	700	13.8	3500	-	1050	13.8
111850	700	13.8	3500	-	1085	13.8
111851	700	13.8	3500	-	1120	13.8
111852	700	13.8	3500	-	1155	13.8
111853	700	13.8	3500	-	1190	13.8
111854	700	13.8	3500	-	1225	13.8
111855	700	13.8	3500	-	1260	13.8
111856	700	13.8	3500	-	1295	13.8
111857	700	13.8	3500	-	1330	13.8
111858	700	13.8	3500	-	1365	13.8
111859	700	13.8	3500	-	1400	13.8
111860	700	13.8	3500	-	1435	13.8
111520	950	13.8	4750	-	987	13.8
112188	550	17.0	2750	1.6	2727	13.7
111576	900	13.7	4500	-	990	13.7
111518	950	13.6	4750	-	893	13.6
111519	950	13.6	4750	-	940	13.6
112094	550	13.5	2750	-	189	13.5
112039	600	13.2	3000	-	1470	13.2
112095	550	13.1	2750	-	216	13.1
111701	800	13.1	4000	-	1000	13.1
111517	950	13.0	4750	-	846	13.0
111216	1300	12.9	6500	-	780	12.9
111574	900	12.9	4500	-	900	12.9
111575	900	12.9	4500	-	945	12.9
111933	650	12.8	3250	-	1056	12.8
111934	650	12.8	3250	-	1088	12.8
111935	650	12.8	3250	-	1120	12.8
111936	650	12.8	3250	-	1152	12.8
111937	650	12.8	3250	-	1184	12.8
111938	650	12.8	3250	-	1216	12.8
111939	650	12.8	3250	-	1248	12.8
111940	650	12.8	3250	-	1280	12.8
111941	650	12.8	3250	-	1312	12.8
111942	650	12.8	3250	-	1344	12.8
111943	650	12.8	3250	-	1376	12.8
111944	650	12.8	3250	-	1408	12.8
111945	650	12.8	3250	-	1440	12.8
111746	750	12.8	3750	-	74	12.8
111573	900	12.7	4500	-	855	12.7
112308	500	15.4	2500	1.4	2775	12.6
111932	650	12.6	3250	-	1024	12.6
111822	700	12.6	3500	-	105	12.6
111996	600	12.6	3000	-	180	12.6
111572	900	12.4	4500	-	810	12.4
112206	500	12.3	2500	-	225	12.3
112207	500	12.3	2500	-	250	12.3
111633	850	12.2	4250	-	882	12.2
111634	850	12.2	4250	-	924	12.2
111635	850	12.2	4250	-	966	12.2
112256	500	12.1	2500	-	1475	12.1
111771	750	12.1	3750	-	999	12.1
111848	700	11.9	3500	-	1015	11.9
111288	1200	11.9	6000	-	780	11.9
112025	600	11.9	3000	-	1050	11.9
112026	600	11.9	3000	-	1080	11.9
112027	600	11.9	3000	-	1110	11.9
112028	600	11.9	3000	-	1140	11.9
112029	600	11.9	3000	-	1170	11.9
112030	600	11.9	3000	-	1200	11.9
112031	600	11.9	3000	-	1230	11.9
112032	600	11.9	3000	-	1260	11.9
112033	600	11.9	3000	-	1290	11.9
112034	600	11.9	3000	-	1320	11.9
112035	600	11.9	3000	-	1350	11.9
112036	600	11.9	3000	-	1380	11.9
112037	600	11.9	3000	-	1410	11.9
112038	600	11.9	3000	-	1440	11.9
111905	650	11.7	3250	-	160	11.7
111632	850	11.7	4250	-	840	11.7
111328	1150	11.6	5750	-	798	11.6
111677	800	11.5	4000	-	40	11.5
111698	800	11.5	4000	-	880	11.5
111699	800	11.5	4000	-	920	11.5
111700	800	11.5	4000	-	960	11.5
112208	500	11.4	2500	-	275	11.4
111612	850	11.1	4250	-	0	11.1
111697	800	11.0	4000	-	840	11.0
112125	550	10.9	2750	-	1026	10.9
112126	550	10.9	2750	-	1053	10.9
112127	550	10.9	2750	-	1080	10.9
112128	550	10.9	2750	-	1107	10.9
112129	550	10.9	2750	-	1134	10.9
112130	550	10.9	2750	-	1161	10.9
112131	550	10.9	2750	-	1188	10.9
112132	550	10.9	2750	-	1215	10.9
112133	550	10.9	2750	-	1242	10.9
112134	550	10.9	2750	-	1269	10.9
112135	550	10.9	2750	-	1296	10.9
112136	550	10.9	2750	-	1323	10.9
112137	550	10.9	2750	-	1350	10.9
112138	550	10.9	2750	-	1377	10.9
112139	550	10.9	2750	-	1404	10.9
112140	550	10.9	2750	-	1431	10.9
112141	550	10.9	2750	-	1458	10.9
111997	600	10.8	3000	-	210	10.8
112024	600	10.8	3000	-	1020	10.8
111768	750	10.7	3750	-	888	10.7
111769	750	10.7	3750	-	925	10.7
111770	750	10.7	3750	-	962	10.7
111767	750	10.5	3750	-	851	10.5
111415	1050	10.4	5250	-	780	10.4
111766	750	10.3	3750	-	814	10.3
111931	650	10.2	3250	-	992	10.2
111847	700	10.2	3500	-	980	10.2
111464	1000	10.1	5000	-	800	10.1
111823	700	10.1	3500	-	140	10.1
112096	550	10.1	2750	-	243	10.1
111844	700	10.0	3500	-	875	10.0
111845	700	10.0	3500	-	910	10.0
111846	700	10.0	3500	-	945	10.0

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
111272	1250	33.8	6250	4.7	2108	24.3
111273	1250	33.8	6250	4.7	2170	24.3
111311	1200	32.4	6000	4.9	2160	22.6
111312	1200	32.4	6000	4.9	2220	22.6
111313	1200	32.4	6000	4.9	2280	22.6
111813	750	23.1	3750	4.7	2553	13.8

Overall score = score_ratio - 2 * repetitive DNA content