Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign121 - back to list

Summary
Image
Unique putative probes (135)
Putative probes (487)
Redundant probes (12)

Summary

`./analyse_probe_search_results : Wed Jun 28 17:20:41 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign121
  assembly      : NCBIM36
  bias          : 3prime
  chromosome    : 15
  id            : 114
  strand        : 1
  -------------

Design window length: 4001bp

All jobs for probe design are successful - total: 66
Fetched conf : Exonerate mouse_NCBIM36 (id: 2)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 1318 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1318/1318
Putative probes that are unique                 : 135/1318
Putative probes exceeding selection criteria    : 634/1318 (both unique and not unique)
Putative probes with successfully-picked primers: 501/634

Identified 12 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
107334	1050	5250	-	1196
107335	1050	5250	-	1248
107392	1000	5000	-	1200
107393	1000	5000	-	1250
107394	1000	5000	-	1300
107454	950	4750	-	1175
107455	950	4750	-	1222
107456	950	4750	-	1269
107457	950	4750	-	1316
107520	900	4500	-	1170
107521	900	4500	-	1215
107522	900	4500	-	1260
107523	900	4500	-	1305
107524	900	4500	-	1350
107525	900	4500	-	1395
107591	850	4250	-	1176
107592	850	4250	-	1218
107593	850	4250	-	1260
107594	850	4250	-	1302
107595	850	4250	-	1344
107596	850	4250	-	1386
107597	850	4250	-	1428
107668	800	4000	-	1160
107669	800	4000	-	1200
107670	800	4000	-	1240
107671	800	4000	-	1280
107672	800	4000	-	1320
107673	800	4000	-	1360
107674	800	4000	-	1400
107675	800	4000	-	1440
107676	800	4000	-	1480
107752	750	3750	-	1184
107753	750	3750	-	1221
107754	750	3750	-	1258
107755	750	3750	-	1295
107756	750	3750	-	1332
107757	750	3750	-	1369
107758	750	3750	-	1406
107759	750	3750	-	1443
107760	750	3750	-	1480
107761	750	3750	-	1517
107842	700	3500	-	1190
107843	700	3500	-	1225
107844	700	3500	-	1260
107845	700	3500	-	1295
107846	700	3500	-	1330
107847	700	3500	-	1365
107848	700	3500	-	1400
107849	700	3500	-	1435
107850	700	3500	-	1470
107851	700	3500	-	1505
107852	700	3500	-	1540
107853	700	3500	-	1575
107940	650	3250	-	1184
107941	650	3250	-	1216
107942	650	3250	-	1248
107943	650	3250	-	1280
107944	650	3250	-	1312
107945	650	3250	-	1344
107946	650	3250	-	1376
107947	650	3250	-	1408
107948	650	3250	-	1440
107949	650	3250	-	1472
107950	650	3250	-	1504
107951	650	3250	-	1536
107952	650	3250	-	1568
107953	650	3250	-	1600
107954	650	3250	-	1632
108047	600	3000	-	1170
108048	600	3000	-	1200
108049	600	3000	-	1230
108050	600	3000	-	1260
108051	600	3000	-	1290
108052	600	3000	-	1320
108053	600	3000	-	1350
108054	600	3000	-	1380
108055	600	3000	-	1410
108056	600	3000	-	1440
108057	600	3000	-	1470
108058	600	3000	-	1500
108059	600	3000	-	1530
108060	600	3000	-	1560
108061	600	3000	-	1590
108062	600	3000	-	1620
108063	600	3000	-	1650
108064	600	3000	-	1680
108165	550	2750	-	1161
108166	550	2750	-	1188
108167	550	2750	-	1215
108168	550	2750	-	1242
108169	550	2750	-	1269
108170	550	2750	-	1296
108171	550	2750	-	1323
108172	550	2750	-	1350
108173	550	2750	-	1377
108174	550	2750	-	1404
108175	550	2750	-	1431
108176	550	2750	-	1458
108177	550	2750	-	1485
108178	550	2750	-	1512
108179	550	2750	-	1539
108180	550	2750	-	1566
108181	550	2750	-	1593
108182	550	2750	-	1620
108183	550	2750	-	1647
108184	550	2750	-	1674
108185	550	2750	-	1701
108186	550	2750	-	1728
108297	500	2500	-	1175
108298	500	2500	-	1200
108299	500	2500	-	1225
108300	500	2500	-	1250
108301	500	2500	-	1275
108302	500	2500	-	1300
108303	500	2500	-	1325
108304	500	2500	-	1350
108305	500	2500	-	1375
108306	500	2500	-	1400
108307	500	2500	-	1425
108308	500	2500	-	1450
108309	500	2500	-	1475
108310	500	2500	-	1500
108311	500	2500	-	1525
108312	500	2500	-	1550
108313	500	2500	-	1575
108314	500	2500	-	1600
108315	500	2500	-	1625
108316	500	2500	-	1650
108317	500	2500	-	1675
108318	500	2500	-	1700
108319	500	2500	-	1725
108320	500	2500	-	1750
108321	500	2500	-	1775
108322	500	2500	-	1800
108279	500	2500	1.0	725

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
107091	1300	43.0	6500	-	1170	43.0
107092	1300	43.0	6500	-	1235	43.0
107093	1300	43.0	6500	-	1300	43.0
107094	1300	41.7	6500	-	1365	41.7
107095	1300	41.7	6500	-	1430	41.7
107096	1300	41.7	6500	-	1495	41.7
107097	1300	41.7	6500	-	1560	41.7
107098	1300	41.7	6500	-	1625	41.7
107099	1300	41.7	6500	-	1690	41.7
107085	1300	41.4	6500	-	780	41.4
107086	1300	41.4	6500	-	845	41.4
107087	1300	41.4	6500	-	910	41.4
107088	1300	41.4	6500	-	975	41.4
107089	1300	41.4	6500	-	1040	41.4
107090	1300	41.4	6500	-	1105	41.4
107134	1250	41.4	6250	-	1178	41.4
107135	1250	41.4	6250	-	1240	41.4
107136	1250	41.4	6250	-	1302	41.4
107137	1250	41.4	6250	-	1364	41.4
107138	1250	40.1	6250	-	1426	40.1
107139	1250	40.1	6250	-	1488	40.1
107140	1250	40.1	6250	-	1550	40.1
107141	1250	40.1	6250	-	1612	40.1
107142	1250	40.1	6250	-	1674	40.1
107143	1250	40.1	6250	-	1736	40.1
107127	1250	39.8	6250	-	744	39.8
107128	1250	39.8	6250	-	806	39.8
107129	1250	39.8	6250	-	868	39.8
107130	1250	39.8	6250	-	930	39.8
107131	1250	39.8	6250	-	992	39.8
107132	1250	39.8	6250	-	1054	39.8
107133	1250	39.8	6250	-	1116	39.8
107180	1200	39.7	6000	-	1200	39.7
107181	1200	39.7	6000	-	1260	39.7
107182	1200	39.7	6000	-	1320	39.7
107183	1200	39.7	6000	-	1380	39.7
107184	1200	39.7	6000	-	1440	39.7
107084	1300	41.4	6500	1.2	715	39.1
107185	1200	38.5	6000	-	1500	38.5
107186	1200	38.5	6000	-	1560	38.5
107187	1200	38.5	6000	-	1620	38.5
107188	1200	38.5	6000	-	1680	38.5
107189	1200	38.5	6000	-	1740	38.5
107190	1200	38.5	6000	-	1800	38.5
107173	1200	38.2	6000	-	780	38.2
107174	1200	38.2	6000	-	840	38.2
107175	1200	38.2	6000	-	900	38.2
107176	1200	38.2	6000	-	960	38.2
107177	1200	38.2	6000	-	1020	38.2
107178	1200	38.2	6000	-	1080	38.2
107179	1200	38.2	6000	-	1140	38.2
107228	1150	38.1	5750	-	1197	38.1
107229	1150	38.1	5750	-	1254	38.1
107230	1150	38.1	5750	-	1311	38.1
107231	1150	38.1	5750	-	1368	38.1
107232	1150	38.1	5750	-	1425	38.1
107233	1150	38.1	5750	-	1482	38.1
107234	1150	36.9	5750	-	1539	36.9
107235	1150	36.9	5750	-	1596	36.9
107236	1150	36.9	5750	-	1653	36.9
107237	1150	36.9	5750	-	1710	36.9
107238	1150	36.9	5750	-	1767	36.9
107239	1150	36.9	5750	-	1824	36.9
107220	1150	36.6	5750	-	741	36.6
107221	1150	36.6	5750	-	798	36.6
107222	1150	36.6	5750	-	855	36.6
107223	1150	36.6	5750	-	912	36.6
107224	1150	36.6	5750	-	969	36.6
107225	1150	36.6	5750	-	1026	36.6
107226	1150	36.6	5750	-	1083	36.6
107227	1150	36.6	5750	-	1140	36.6
107172	1200	38.2	6000	0.8	720	36.5
107280	1100	36.4	5500	-	1210	36.4
107281	1100	36.4	5500	-	1265	36.4
107282	1100	36.4	5500	-	1320	36.4
107283	1100	36.4	5500	-	1375	36.4
107284	1100	36.4	5500	-	1430	36.4
107285	1100	36.4	5500	-	1485	36.4
107286	1100	36.4	5500	-	1540	36.4
107287	1100	35.3	5500	-	1595	35.3
107288	1100	35.3	5500	-	1650	35.3
107289	1100	35.3	5500	-	1705	35.3
107290	1100	35.3	5500	-	1760	35.3
107291	1100	35.3	5500	-	1815	35.3
107292	1100	35.3	5500	-	1870	35.3
107272	1100	35.0	5500	-	770	35.0
107273	1100	35.0	5500	-	825	35.0
107274	1100	35.0	5500	-	880	35.0
107275	1100	35.0	5500	-	935	35.0
107276	1100	35.0	5500	-	990	35.0
107277	1100	35.0	5500	-	1045	35.0
107278	1100	35.0	5500	-	1100	35.0
107279	1100	35.0	5500	-	1155	35.0
107336	1050	34.8	5250	-	1300	34.8
107337	1050	34.8	5250	-	1352	34.8
107338	1050	34.8	5250	-	1404	34.8
107339	1050	34.8	5250	-	1456	34.8
107340	1050	34.8	5250	-	1508	34.8
107341	1050	34.8	5250	-	1560	34.8
107240	1150	36.9	5750	1.4	1881	34.1
107144	1250	39.3	6250	2.6	1798	34.0
107342	1050	33.7	5250	-	1612	33.7
107343	1050	33.7	5250	-	1664	33.7
107344	1050	33.7	5250	-	1716	33.7
107345	1050	33.7	5250	-	1768	33.7
107346	1050	33.7	5250	-	1820	33.7
107347	1050	33.7	5250	-	1872	33.7
107348	1050	33.7	5250	-	1924	33.7
107326	1050	33.4	5250	-	780	33.4
107327	1050	33.4	5250	-	832	33.4
107328	1050	33.4	5250	-	884	33.4
107329	1050	33.4	5250	-	936	33.4
107330	1050	33.4	5250	-	988	33.4
107331	1050	33.4	5250	-	1040	33.4
107332	1050	33.4	5250	-	1092	33.4
107333	1050	33.4	5250	-	1144	33.4
107293	1100	35.3	5500	0.9	1925	33.4
107395	1000	33.1	5000	-	1350	33.1
107396	1000	33.1	5000	-	1400	33.1
107397	1000	33.1	5000	-	1450	33.1
107398	1000	33.1	5000	-	1500	33.1
107399	1000	33.1	5000	-	1550	33.1
107400	1000	33.1	5000	-	1600	33.1
107401	1000	33.1	5000	-	1650	33.1
107325	1050	33.4	5250	0.2	728	33.1
107402	1000	32.1	5000	-	1700	32.1
107403	1000	32.1	5000	-	1750	32.1
107404	1000	32.1	5000	-	1800	32.1
107405	1000	32.1	5000	-	1850	32.1
107406	1000	32.1	5000	-	1900	32.1
107407	1000	32.1	5000	-	1950	32.1
107408	1000	32.1	5000	-	2000	32.1
107383	1000	31.8	5000	-	750	31.8
107384	1000	31.8	5000	-	800	31.8
107385	1000	31.8	5000	-	850	31.8
107386	1000	31.8	5000	-	900	31.8
107387	1000	31.8	5000	-	950	31.8
107388	1000	31.8	5000	-	1000	31.8
107389	1000	31.8	5000	-	1050	31.8
107390	1000	31.8	5000	-	1100	31.8
107391	1000	31.8	5000	-	1150	31.8
107349	1050	33.7	5250	1.0	1976	31.6
107458	950	31.5	4750	-	1363	31.5
107459	950	31.5	4750	-	1410	31.5
107460	950	31.5	4750	-	1457	31.5
107461	950	31.5	4750	-	1504	31.5
107462	950	31.5	4750	-	1551	31.5
107463	950	31.5	4750	-	1598	31.5
107464	950	31.5	4750	-	1645	31.5
107465	950	31.5	4750	-	1692	31.5
107466	950	30.4	4750	-	1739	30.4
107467	950	30.4	4750	-	1786	30.4
107468	950	30.4	4750	-	1833	30.4
107469	950	30.4	4750	-	1880	30.4
107470	950	30.4	4750	-	1927	30.4
107471	950	30.4	4750	-	1974	30.4
107472	950	30.4	4750	-	2021	30.4
107445	950	30.3	4750	-	752	30.3
107446	950	30.3	4750	-	799	30.3
107447	950	30.3	4750	-	846	30.3
107448	950	30.3	4750	-	893	30.3
107449	950	30.3	4750	-	940	30.3
107450	950	30.3	4750	-	987	30.3
107451	950	30.3	4750	-	1034	30.3
107452	950	30.3	4750	-	1081	30.3
107453	950	30.3	4750	-	1128	30.3
107473	950	30.4	4750	0.3	2068	29.8
107526	900	29.8	4500	-	1440	29.8
107527	900	29.8	4500	-	1485	29.8
107528	900	29.8	4500	-	1530	29.8
107529	900	29.8	4500	-	1575	29.8
107530	900	29.8	4500	-	1620	29.8
107531	900	29.8	4500	-	1665	29.8
107532	900	29.8	4500	-	1710	29.8
107100	1300	35.1	6500	3.1	1755	29.0
107533	900	28.8	4500	-	1755	28.8
107534	900	28.8	4500	-	1800	28.8
107535	900	28.8	4500	-	1845	28.8
107536	900	28.8	4500	-	1890	28.8
107537	900	28.8	4500	-	1935	28.8
107538	900	28.8	4500	-	1980	28.8
107539	900	28.8	4500	-	2025	28.8
107540	900	28.8	4500	-	2070	28.8
107541	900	28.8	4500	-	2115	28.8
107511	900	28.7	4500	-	765	28.7
107512	900	28.7	4500	-	810	28.7
107513	900	28.7	4500	-	855	28.7
107514	900	28.7	4500	-	900	28.7
107515	900	28.7	4500	-	945	28.7
107516	900	28.7	4500	-	990	28.7
107517	900	28.7	4500	-	1035	28.7
107518	900	28.7	4500	-	1080	28.7
107519	900	28.7	4500	-	1125	28.7
107598	850	28.1	4250	-	1470	28.1
107599	850	28.1	4250	-	1512	28.1
107600	850	28.1	4250	-	1554	28.1
107601	850	28.1	4250	-	1596	28.1
107602	850	28.1	4250	-	1638	28.1
107603	850	28.1	4250	-	1680	28.1
107604	850	28.1	4250	-	1722	28.1
107605	850	28.1	4250	-	1764	28.1
107606	850	27.2	4250	-	1806	27.2
107607	850	27.2	4250	-	1848	27.2
107608	850	27.2	4250	-	1890	27.2
107609	850	27.2	4250	-	1932	27.2
107610	850	27.2	4250	-	1974	27.2
107611	850	27.2	4250	-	2016	27.2
107612	850	27.2	4250	-	2058	27.2
107613	850	27.2	4250	-	2100	27.2
107614	850	27.2	4250	-	2142	27.2
107581	850	27.1	4250	-	756	27.1
107582	850	27.1	4250	-	798	27.1
107583	850	27.1	4250	-	840	27.1
107584	850	27.1	4250	-	882	27.1
107585	850	27.1	4250	-	924	27.1
107586	850	27.1	4250	-	966	27.1
107587	850	27.1	4250	-	1008	27.1
107588	850	27.1	4250	-	1050	27.1
107589	850	27.1	4250	-	1092	27.1
107590	850	27.1	4250	-	1134	27.1
107677	800	26.5	4000	-	1520	26.5
107678	800	26.5	4000	-	1560	26.5
107679	800	26.5	4000	-	1600	26.5
107680	800	26.5	4000	-	1640	26.5
107681	800	26.5	4000	-	1680	26.5
107682	800	26.5	4000	-	1720	26.5
107683	800	26.5	4000	-	1760	26.5
107684	800	26.5	4000	-	1800	26.5
107685	800	26.5	4000	-	1840	26.5
107510	900	28.7	4500	1.1	720	26.4
107382	1000	31.8	5000	3.0	700	25.8
107686	800	25.6	4000	-	1880	25.6
107687	800	25.6	4000	-	1920	25.6
107688	800	25.6	4000	-	1960	25.6
107689	800	25.6	4000	-	2000	25.6
107690	800	25.6	4000	-	2040	25.6
107691	800	25.6	4000	-	2080	25.6
107692	800	25.6	4000	-	2120	25.6
107693	800	25.6	4000	-	2160	25.6
107694	800	25.6	4000	-	2200	25.6
107658	800	25.5	4000	-	760	25.5
107659	800	25.5	4000	-	800	25.5
107660	800	25.5	4000	-	840	25.5
107661	800	25.5	4000	-	880	25.5
107662	800	25.5	4000	-	920	25.5
107663	800	25.5	4000	-	960	25.5
107664	800	25.5	4000	-	1000	25.5
107665	800	25.5	4000	-	1040	25.5
107666	800	25.5	4000	-	1080	25.5
107667	800	25.5	4000	-	1120	25.5
107444	950	30.3	4750	2.6	705	25.0
107762	750	24.8	3750	-	1554	24.8
107763	750	24.8	3750	-	1591	24.8
107764	750	24.8	3750	-	1628	24.8
107765	750	24.8	3750	-	1665	24.8
107766	750	24.8	3750	-	1702	24.8
107767	750	24.8	3750	-	1739	24.8
107768	750	24.8	3750	-	1776	24.8
107769	750	24.8	3750	-	1813	24.8
107770	750	24.8	3750	-	1850	24.8
107771	750	24.8	3750	-	1887	24.8
107772	750	24.0	3750	-	1924	24.0
107773	750	24.0	3750	-	1961	24.0
107774	750	24.0	3750	-	1998	24.0
107775	750	24.0	3750	-	2035	24.0
107776	750	24.0	3750	-	2072	24.0
107777	750	24.0	3750	-	2109	24.0
107778	750	24.0	3750	-	2146	24.0
107779	750	24.0	3750	-	2183	24.0
107780	750	24.0	3750	-	2220	24.0
107781	750	24.0	3750	-	2257	24.0
107740	750	23.9	3750	-	740	23.9
107741	750	23.9	3750	-	777	23.9
107742	750	23.9	3750	-	814	23.9
107743	750	23.9	3750	-	851	23.9
107744	750	23.9	3750	-	888	23.9
107745	750	23.9	3750	-	925	23.9
107746	750	23.9	3750	-	962	23.9
107747	750	23.9	3750	-	999	23.9
107748	750	23.9	3750	-	1036	23.9
107749	750	23.9	3750	-	1073	23.9
107750	750	23.9	3750	-	1110	23.9
107751	750	23.9	3750	-	1147	23.9
107580	850	27.1	4250	1.9	714	23.3
107854	700	23.2	3500	-	1610	23.2
107855	700	23.2	3500	-	1645	23.2
107856	700	23.2	3500	-	1680	23.2
107857	700	23.2	3500	-	1715	23.2
107858	700	23.2	3500	-	1750	23.2
107859	700	23.2	3500	-	1785	23.2
107860	700	23.2	3500	-	1820	23.2
107861	700	23.2	3500	-	1855	23.2
107862	700	23.2	3500	-	1890	23.2
107863	700	23.2	3500	-	1925	23.2
107191	1200	30.6	6000	3.8	1860	23.1
107615	850	27.2	4250	2.2	2184	22.8
107864	700	22.4	3500	-	1960	22.4
107865	700	22.4	3500	-	1995	22.4
107866	700	22.4	3500	-	2030	22.4
107867	700	22.4	3500	-	2065	22.4
107868	700	22.4	3500	-	2100	22.4
107869	700	22.4	3500	-	2135	22.4
107870	700	22.4	3500	-	2170	22.4
107871	700	22.4	3500	-	2205	22.4
107872	700	22.4	3500	-	2240	22.4
107873	700	22.4	3500	-	2275	22.4
107874	700	22.4	3500	-	2310	22.4
107126	1250	30.0	6250	3.8	682	22.4
107829	700	22.3	3500	-	735	22.3
107830	700	22.3	3500	-	770	22.3
107831	700	22.3	3500	-	805	22.3
107832	700	22.3	3500	-	840	22.3
107833	700	22.3	3500	-	875	22.3
107834	700	22.3	3500	-	910	22.3
107835	700	22.3	3500	-	945	22.3
107836	700	22.3	3500	-	980	22.3
107837	700	22.3	3500	-	1015	22.3
107838	700	22.3	3500	-	1050	22.3
107839	700	22.3	3500	-	1085	22.3
107840	700	22.3	3500	-	1120	22.3
107841	700	22.3	3500	-	1155	22.3
107955	650	21.5	3250	-	1664	21.5
107956	650	21.5	3250	-	1696	21.5
107957	650	21.5	3250	-	1728	21.5
107958	650	21.5	3250	-	1760	21.5
107959	650	21.5	3250	-	1792	21.5
107960	650	21.5	3250	-	1824	21.5
107961	650	21.5	3250	-	1856	21.5
107962	650	21.5	3250	-	1888	21.5
107963	650	21.5	3250	-	1920	21.5
107964	650	21.5	3250	-	1952	21.5
107965	650	21.5	3250	-	1984	21.5
107966	650	20.8	3250	-	2016	20.8
107967	650	20.8	3250	-	2048	20.8
107968	650	20.8	3250	-	2080	20.8
107969	650	20.8	3250	-	2112	20.8
107970	650	20.8	3250	-	2144	20.8
107971	650	20.8	3250	-	2176	20.8
107972	650	20.8	3250	-	2208	20.8
107973	650	20.8	3250	-	2240	20.8
107974	650	20.8	3250	-	2272	20.8
107975	650	20.8	3250	-	2304	20.8
107976	650	20.8	3250	-	2336	20.8
107926	650	20.7	3250	-	736	20.7
107927	650	20.7	3250	-	768	20.7
107928	650	20.7	3250	-	800	20.7
107929	650	20.7	3250	-	832	20.7
107930	650	20.7	3250	-	864	20.7
107931	650	20.7	3250	-	896	20.7
107932	650	20.7	3250	-	928	20.7
107933	650	20.7	3250	-	960	20.7
107934	650	20.7	3250	-	992	20.7
107935	650	20.7	3250	-	1024	20.7
107936	650	20.7	3250	-	1056	20.7
107937	650	20.7	3250	-	1088	20.7
107938	650	20.7	3250	-	1120	20.7
107939	650	20.7	3250	-	1152	20.7
107977	650	20.8	3250	0.5	2368	19.9
108065	600	19.9	3000	-	1710	19.9
108066	600	19.9	3000	-	1740	19.9
108067	600	19.9	3000	-	1770	19.9
108068	600	19.9	3000	-	1800	19.9
108069	600	19.9	3000	-	1830	19.9
108070	600	19.9	3000	-	1860	19.9
108071	600	19.9	3000	-	1890	19.9
108072	600	19.9	3000	-	1920	19.9
108073	600	19.9	3000	-	1950	19.9
108074	600	19.9	3000	-	1980	19.9
108075	600	19.9	3000	-	2010	19.9
108076	600	19.9	3000	-	2040	19.9
108077	600	19.2	3000	-	2070	19.2
108078	600	19.2	3000	-	2100	19.2
108079	600	19.2	3000	-	2130	19.2
108080	600	19.2	3000	-	2160	19.2
108081	600	19.2	3000	-	2190	19.2
108082	600	19.2	3000	-	2220	19.2
108083	600	19.2	3000	-	2250	19.2
108084	600	19.2	3000	-	2280	19.2
108085	600	19.2	3000	-	2310	19.2
108086	600	19.2	3000	-	2340	19.2
108087	600	19.2	3000	-	2370	19.2
108088	600	19.2	3000	-	2400	19.2
108033	600	19.1	3000	-	750	19.1
108034	600	19.1	3000	-	780	19.1
108035	600	19.1	3000	-	810	19.1
108036	600	19.1	3000	-	840	19.1
108037	600	19.1	3000	-	870	19.1
108038	600	19.1	3000	-	900	19.1
108039	600	19.1	3000	-	930	19.1
108040	600	19.1	3000	-	960	19.1
108041	600	19.1	3000	-	990	19.1
108042	600	19.1	3000	-	1020	19.1
108043	600	19.1	3000	-	1050	19.1
108044	600	19.1	3000	-	1080	19.1
108045	600	19.1	3000	-	1110	19.1
108046	600	19.1	3000	-	1140	19.1
108187	550	18.2	2750	-	1755	18.2
108188	550	18.2	2750	-	1782	18.2
108189	550	18.2	2750	-	1809	18.2
108190	550	18.2	2750	-	1836	18.2
108191	550	18.2	2750	-	1863	18.2
108192	550	18.2	2750	-	1890	18.2
108193	550	18.2	2750	-	1917	18.2
108194	550	18.2	2750	-	1944	18.2
108195	550	18.2	2750	-	1971	18.2
108196	550	18.2	2750	-	1998	18.2
108197	550	18.2	2750	-	2025	18.2
108198	550	18.2	2750	-	2052	18.2
108199	550	18.2	2750	-	2079	18.2
108200	550	17.6	2750	-	2106	17.6
108201	550	17.6	2750	-	2133	17.6
108202	550	17.6	2750	-	2160	17.6
108203	550	17.6	2750	-	2187	17.6
108204	550	17.6	2750	-	2214	17.6
108205	550	17.6	2750	-	2241	17.6
108206	550	17.6	2750	-	2268	17.6
108207	550	17.6	2750	-	2295	17.6
108208	550	17.6	2750	-	2322	17.6
108209	550	17.6	2750	-	2349	17.6
108210	550	17.6	2750	-	2376	17.6
108211	550	17.6	2750	-	2403	17.6
108212	550	17.6	2750	-	2430	17.6
108213	550	17.6	2750	-	2457	17.6
108150	550	17.5	2750	-	756	17.5
108151	550	17.5	2750	-	783	17.5
108152	550	17.5	2750	-	810	17.5
108153	550	17.5	2750	-	837	17.5
108154	550	17.5	2750	-	864	17.5
108155	550	17.5	2750	-	891	17.5
108156	550	17.5	2750	-	918	17.5
108157	550	17.5	2750	-	945	17.5
108158	550	17.5	2750	-	972	17.5
108159	550	17.5	2750	-	999	17.5
108160	550	17.5	2750	-	1026	17.5
108161	550	17.5	2750	-	1053	17.5
108162	550	17.5	2750	-	1080	17.5
108163	550	17.5	2750	-	1107	17.5
108164	550	17.5	2750	-	1134	17.5
108149	550	17.5	2750	0.2	729	17.2
107739	750	23.9	3750	3.6	703	16.7
108323	500	16.6	2500	-	1825	16.6
108324	500	16.6	2500	-	1850	16.6
108325	500	16.6	2500	-	1875	16.6
108326	500	16.6	2500	-	1900	16.6
108327	500	16.6	2500	-	1925	16.6
108328	500	16.6	2500	-	1950	16.6
108329	500	16.6	2500	-	1975	16.6
108330	500	16.6	2500	-	2000	16.6
108331	500	16.6	2500	-	2025	16.6
108332	500	16.6	2500	-	2050	16.6
108333	500	16.6	2500	-	2075	16.6
108334	500	16.6	2500	-	2100	16.6
108335	500	16.6	2500	-	2125	16.6
108336	500	16.6	2500	-	2150	16.6
108337	500	16.0	2500	-	2175	16.0
108338	500	16.0	2500	-	2200	16.0
108339	500	16.0	2500	-	2225	16.0
108340	500	16.0	2500	-	2250	16.0
108341	500	16.0	2500	-	2275	16.0
108342	500	16.0	2500	-	2300	16.0
108343	500	16.0	2500	-	2325	16.0
108344	500	16.0	2500	-	2350	16.0
108345	500	16.0	2500	-	2375	16.0
108346	500	16.0	2500	-	2400	16.0
108347	500	16.0	2500	-	2425	16.0
108348	500	16.0	2500	-	2450	16.0
108349	500	16.0	2500	-	2475	16.0
108350	500	16.0	2500	-	2500	16.0
108280	500	15.9	2500	-	750	15.9
108281	500	15.9	2500	-	775	15.9
108282	500	15.9	2500	-	800	15.9
108283	500	15.9	2500	-	825	15.9
108284	500	15.9	2500	-	850	15.9
108285	500	15.9	2500	-	875	15.9
108286	500	15.9	2500	-	900	15.9
108287	500	15.9	2500	-	925	15.9
108288	500	15.9	2500	-	950	15.9
108289	500	15.9	2500	-	975	15.9
108290	500	15.9	2500	-	1000	15.9
108291	500	15.9	2500	-	1025	15.9
108292	500	15.9	2500	-	1050	15.9
108293	500	15.9	2500	-	1075	15.9
108294	500	15.9	2500	-	1100	15.9
108295	500	15.9	2500	-	1125	15.9
108296	500	15.9	2500	-	1150	15.9
108089	600	19.2	3000	2.5	2430	14.2
108351	500	16.0	2500	2.0	2525	12.0

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
107271	1100	35.0	5500	1.4	715	32.3
107657	800	25.5	4000	1.2	720	23.0
107409	1000	29.6	5000	3.5	2050	22.6
107219	1150	29.0	5750	4.0	684	21.0
107695	800	25.6	4000	3.1	2240	19.4
107782	750	24.0	3750	3.9	2294	16.3
108032	600	19.1	3000	1.7	720	15.8
107875	700	22.4	3500	4.3	2345	13.9
107828	700	22.3	3500	4.3	700	13.7
107542	900	23.0	4500	5.0	2160	13.0
107925	650	20.7	3250	4.0	704	12.7
108214	550	17.6	2750	3.5	2484	10.7

Overall score = score_ratio - 2 * repetitive DNA content