Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign122 - back to list

Summary
Image
Unique putative probes (24)
Putative probes (191)
Redundant probes (272)

Summary

`./analyse_probe_search_results : Wed Jun 28 17:22:36 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign122
  assembly      : NCBIM36
  bias          : 3prime
  chromosome    : 11
  id            : 116
  strand        : 1
  -------------

Design window length: 2501bp

All jobs for probe design are successful - total: 35
Fetched conf : Exonerate mouse_NCBIM36 (id: 2)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 700 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 700/700
Putative probes that are unique                 : 24/700
Putative probes exceeding selection criteria    : 487/700 (both unique and not unique)
Putative probes with successfully-picked primers: 480/487

Identified 272 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
108948	650	3250	-	1216
108949	650	3250	-	1248
109008	600	3000	-	1200
109009	600	3000	-	1230
109010	600	3000	-	1260
109011	600	3000	-	1290
109077	550	2750	-	1215
109078	550	2750	-	1242
109079	550	2750	-	1269
109080	550	2750	-	1296
109081	550	2750	-	1323
109082	550	2750	-	1350
109105	500	2500	-	0
109106	500	2500	-	25
109153	500	2500	-	1200
109154	500	2500	-	1225
109155	500	2500	-	1250
109156	500	2500	-	1275
109157	500	2500	-	1300
109158	500	2500	-	1325
109159	500	2500	-	1350
109160	500	2500	-	1375
109161	500	2500	-	1400
109012	600	3000	1.5	1320

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
108643	1000	27.2	5000	-	850	27.2
108644	1000	27.2	5000	-	900	27.2
108676	950	25.8	4750	-	893	25.8
108677	950	25.8	4750	-	940	25.8
108710	900	24.5	4500	-	855	24.5
108711	900	24.5	4500	-	900	24.5
108712	900	24.5	4500	-	945	24.5
108713	900	24.5	4500	-	990	24.5
108675	950	23.5	4750	-	846	23.5
108748	850	23.1	4250	-	882	23.1
108749	850	23.1	4250	-	924	23.1
108750	850	23.1	4250	-	966	23.1
108751	850	23.1	4250	-	1008	23.1
108752	850	23.1	4250	-	1050	23.1
108499	1300	26.5	6500	1.8	845	22.8
108500	1300	26.5	6500	1.8	910	22.8
108501	1300	26.5	6500	1.8	975	22.8
108502	1300	26.5	6500	1.8	1040	22.8
108503	1300	26.5	6500	1.8	1105	22.8
108504	1300	26.5	6500	1.8	1170	22.8
108614	1050	22.8	5250	-	832	22.8
108789	800	21.7	4000	-	880	21.7
108790	800	21.7	4000	-	920	21.7
108791	800	21.7	4000	-	960	21.7
108792	800	21.7	4000	-	1000	21.7
108793	800	21.7	4000	-	1040	21.7
108794	800	21.7	4000	-	1080	21.7
108525	1250	25.5	6250	1.9	1240	21.7
108587	1100	23.9	5500	1.3	825	21.4
108833	750	20.4	3750	-	851	20.4
108834	750	20.4	3750	-	888	20.4
108835	750	20.4	3750	-	925	20.4
108836	750	20.4	3750	-	962	20.4
108837	750	20.4	3750	-	999	20.4
108838	750	20.4	3750	-	1036	20.4
108839	750	20.4	3750	-	1073	20.4
108840	750	20.4	3750	-	1110	20.4
108841	750	20.4	3750	-	1147	20.4
108642	1000	19.8	5000	-	800	19.8
108795	800	21.7	4000	1.1	1120	19.5
108586	1100	19.3	5500	-	770	19.3
108498	1300	23.0	6500	1.8	780	19.3
108747	850	19.1	4250	-	840	19.1
108883	700	19.0	3500	-	875	19.0
108884	700	19.0	3500	-	910	19.0
108885	700	19.0	3500	-	945	19.0
108886	700	19.0	3500	-	980	19.0
108887	700	19.0	3500	-	1015	19.0
108888	700	19.0	3500	-	1050	19.0
108889	700	19.0	3500	-	1085	19.0
108890	700	19.0	3500	-	1120	19.0
108891	700	19.0	3500	-	1155	19.0
108892	700	19.0	3500	-	1190	19.0
108674	950	18.8	4750	-	799	18.8
108613	1050	18.6	5250	-	780	18.6
109104	550	18.3	2750	-	1944	18.3
108709	900	18.3	4500	-	810	18.3
108788	800	17.9	4000	-	840	17.9
108937	650	17.7	3250	-	864	17.7
108938	650	17.7	3250	-	896	17.7
108939	650	17.7	3250	-	928	17.7
108940	650	17.7	3250	-	960	17.7
108941	650	17.7	3250	-	992	17.7
108942	650	17.7	3250	-	1024	17.7
108943	650	17.7	3250	-	1056	17.7
108944	650	17.7	3250	-	1088	17.7
108945	650	17.7	3250	-	1120	17.7
108946	650	17.7	3250	-	1152	17.7
108947	650	17.7	3250	-	1184	17.7
108495	1300	17.1	6500	-	585	17.1
108746	850	16.8	4250	-	798	16.8
109183	500	16.7	2500	-	1950	16.7
109184	500	16.7	2500	-	1975	16.7
109185	500	16.7	2500	-	2000	16.7
108515	1250	16.4	6250	-	620	16.4
108561	1150	16.3	5750	-	741	16.3
108997	600	16.3	3000	-	870	16.3
108998	600	16.3	3000	-	900	16.3
108999	600	16.3	3000	-	930	16.3
109000	600	16.3	3000	-	960	16.3
109001	600	16.3	3000	-	990	16.3
109002	600	16.3	3000	-	1020	16.3
109003	600	16.3	3000	-	1050	16.3
109004	600	16.3	3000	-	1080	16.3
109005	600	16.3	3000	-	1110	16.3
109006	600	16.3	3000	-	1140	16.3
109007	600	16.3	3000	-	1170	16.3
108832	750	15.9	3750	-	814	15.9
108787	800	15.9	4000	-	800	15.9
108536	1200	15.7	6000	-	600	15.7
108537	1200	15.7	6000	-	660	15.7
108882	700	15.7	3500	-	840	15.7
108656	1000	20.4	5000	2.4	1500	15.6
108641	1000	15.5	5000	-	750	15.5
108559	1150	15.1	5750	-	627	15.1
108560	1150	15.1	5750	-	684	15.1
108673	950	15.0	4750	-	752	15.0
108708	900	15.0	4500	-	765	15.0
109064	550	14.9	2750	-	864	14.9
109065	550	14.9	2750	-	891	14.9
109066	550	14.9	2750	-	918	14.9
109067	550	14.9	2750	-	945	14.9
109068	550	14.9	2750	-	972	14.9
109069	550	14.9	2750	-	999	14.9
109070	550	14.9	2750	-	1026	14.9
109071	550	14.9	2750	-	1053	14.9
109072	550	14.9	2750	-	1080	14.9
109073	550	14.9	2750	-	1107	14.9
109074	550	14.9	2750	-	1134	14.9
109075	550	14.9	2750	-	1161	14.9
109076	550	14.9	2750	-	1188	14.9
108612	1050	14.6	5250	-	728	14.6
108583	1100	14.4	5500	-	605	14.4
108584	1100	14.4	5500	-	660	14.4
108585	1100	14.4	5500	-	715	14.4
108690	950	19.4	4750	2.5	1551	14.3
108538	1200	15.7	6000	0.8	720	14.2
108881	700	14.2	3500	-	805	14.2
108936	650	14.1	3250	-	832	14.1
108558	1150	15.1	5750	0.6	570	13.9
108610	1050	13.8	5250	-	624	13.8
108611	1050	13.8	5250	-	676	13.8
108745	850	13.6	4250	-	756	13.6
109139	500	13.6	2500	-	850	13.6
109140	500	13.6	2500	-	875	13.6
109141	500	13.6	2500	-	900	13.6
109142	500	13.6	2500	-	925	13.6
109143	500	13.6	2500	-	950	13.6
109144	500	13.6	2500	-	975	13.6
109145	500	13.6	2500	-	1000	13.6
109146	500	13.6	2500	-	1025	13.6
109147	500	13.6	2500	-	1050	13.6
109148	500	13.6	2500	-	1075	13.6
109149	500	13.6	2500	-	1100	13.6
109150	500	13.6	2500	-	1125	13.6
109151	500	13.6	2500	-	1150	13.6
109152	500	13.6	2500	-	1175	13.6
108996	600	13.5	3000	-	840	13.5
108496	1300	17.1	6500	1.8	650	13.4
108497	1300	17.1	6500	1.8	715	13.4
108514	1250	16.4	6250	1.5	558	13.4
108831	750	13.3	3750	-	777	13.3
108638	1000	13.1	5000	-	600	13.1
108639	1000	13.1	5000	-	650	13.1
108640	1000	13.1	5000	-	700	13.1
108516	1250	16.4	6250	1.7	682	13.0
108935	650	12.9	3250	-	800	12.9
108609	1050	13.8	5250	0.5	572	12.8
108786	800	12.8	4000	-	760	12.8
108670	950	12.5	4750	-	611	12.5
108671	950	12.5	4750	-	658	12.5
108672	950	12.5	4750	-	705	12.5
108880	700	12.3	3500	-	770	12.3
108995	600	12.2	3000	-	810	12.2
109063	550	12.0	2750	-	837	12.0
108704	900	11.8	4500	-	585	11.8
108705	900	11.8	4500	-	630	11.8
108706	900	11.8	4500	-	675	11.8
108707	900	11.8	4500	-	720	11.8
109062	550	11.2	2750	-	810	11.2
108741	850	11.2	4250	-	588	11.2
108742	850	11.2	4250	-	630	11.2
108743	850	11.2	4250	-	672	11.2
108744	850	11.2	4250	-	714	11.2
108934	650	11.0	3250	-	768	11.0
109138	500	10.9	2500	-	825	10.9
108830	750	10.7	3750	-	740	10.7
108994	600	10.6	3000	-	780	10.6
108782	800	10.5	4000	-	600	10.5
108783	800	10.5	4000	-	640	10.5
108784	800	10.5	4000	-	680	10.5
108785	800	10.5	4000	-	720	10.5
108669	950	12.5	4750	1.4	564	9.7
108535	1200	15.7	6000	3.1	540	9.6
108582	1100	14.4	5500	2.5	550	9.5
108486	1300	17.1	6500	4.0	0	9.1
108487	1300	17.1	6500	4.0	65	9.1
108488	1300	17.1	6500	4.0	130	9.1
108489	1300	17.1	6500	4.0	195	9.1
108490	1300	17.1	6500	4.0	260	9.1
108491	1300	17.1	6500	4.0	325	9.1
108492	1300	17.1	6500	4.0	390	9.1
108493	1300	17.1	6500	4.0	455	9.1
108494	1300	17.1	6500	4.0	520	9.1
108506	1250	16.4	6250	4.2	62	8.1
108507	1250	16.4	6250	4.2	124	8.1
108508	1250	16.4	6250	4.2	186	8.1
108509	1250	16.4	6250	4.2	248	8.1
108637	1000	13.1	5000	2.7	550	7.7
108781	800	10.5	4000	2.1	560	6.2
108740	850	11.2	4250	3.6	546	3.9

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
108615	1050	26.4	5250	2.2	884	22.0
108519	1250	25.5	6250	1.9	868	21.7
108520	1250	25.5	6250	1.9	930	21.7
108521	1250	25.5	6250	1.9	992	21.7
108522	1250	25.5	6250	1.9	1054	21.7
108523	1250	25.5	6250	1.9	1116	21.7
108524	1250	25.5	6250	1.9	1178	21.7
108518	1250	25.4	6250	1.9	806	21.6
108540	1200	24.5	6000	2.0	840	20.5
108541	1200	24.5	6000	2.0	900	20.5
108542	1200	24.5	6000	2.0	960	20.5
108543	1200	24.5	6000	2.0	1020	20.5
108544	1200	24.5	6000	2.0	1080	20.5
108545	1200	24.5	6000	2.0	1140	20.5
108546	1200	24.5	6000	2.0	1200	20.5
108547	1200	24.5	6000	2.0	1260	20.5
108563	1150	23.5	5750	2.1	855	19.3
108564	1150	23.5	5750	2.1	912	19.3
108565	1150	23.5	5750	2.1	969	19.3
108566	1150	23.5	5750	2.1	1026	19.3
108567	1150	23.5	5750	2.1	1083	19.3
108568	1150	23.5	5750	2.1	1140	19.3
108569	1150	23.5	5750	2.1	1197	19.3
108570	1150	23.5	5750	2.1	1254	19.3
108571	1150	23.5	5750	2.1	1311	19.3
108645	1000	23.6	5000	2.4	950	18.8
108893	700	22.7	3500	2.0	1225	18.7
108562	1150	22.7	5750	2.1	798	18.6
108678	950	23.3	4750	2.5	987	18.2
108588	1100	22.4	5500	2.2	880	18.1
108589	1100	22.4	5500	2.2	935	18.1
108590	1100	22.4	5500	2.2	990	18.1
108591	1100	22.4	5500	2.2	1045	18.1
108592	1100	22.4	5500	2.2	1100	18.1
108593	1100	22.4	5500	2.2	1155	18.1
108594	1100	22.4	5500	2.2	1210	18.1
108595	1100	22.4	5500	2.2	1265	18.1
108596	1100	22.4	5500	2.2	1320	18.1
108597	1100	22.4	5500	2.2	1375	18.1
108539	1200	21.2	6000	2.0	780	17.2
108616	1050	21.4	5250	2.3	936	16.9
108617	1050	21.4	5250	2.3	988	16.9
108618	1050	21.4	5250	2.3	1040	16.9
108619	1050	21.4	5250	2.3	1092	16.9
108620	1050	21.4	5250	2.3	1144	16.9
108621	1050	21.4	5250	2.3	1196	16.9
108622	1050	21.4	5250	2.3	1248	16.9
108623	1050	21.4	5250	2.3	1300	16.9
108624	1050	21.4	5250	2.3	1352	16.9
108625	1050	21.4	5250	2.3	1404	16.9
108714	900	22.1	4500	2.7	1035	16.7
108646	1000	20.4	5000	2.4	1000	15.6
108647	1000	20.4	5000	2.4	1050	15.6
108648	1000	20.4	5000	2.4	1100	15.6
108649	1000	20.4	5000	2.4	1150	15.6
108650	1000	20.4	5000	2.4	1200	15.6
108651	1000	20.4	5000	2.4	1250	15.6
108652	1000	20.4	5000	2.4	1300	15.6
108653	1000	20.4	5000	2.4	1350	15.6
108654	1000	20.4	5000	2.4	1400	15.6
108655	1000	20.4	5000	2.4	1450	15.6
108753	850	20.8	4250	2.8	1092	15.2
108679	950	19.4	4750	2.5	1034	14.3
108680	950	19.4	4750	2.5	1081	14.3
108681	950	19.4	4750	2.5	1128	14.3
108682	950	19.4	4750	2.5	1175	14.3
108683	950	19.4	4750	2.5	1222	14.3
108684	950	19.4	4750	2.5	1269	14.3
108685	950	19.4	4750	2.5	1316	14.3
108686	950	19.4	4750	2.5	1363	14.3
108687	950	19.4	4750	2.5	1410	14.3
108688	950	19.4	4750	2.5	1457	14.3
108689	950	19.4	4750	2.5	1504	14.3
108517	1250	17.8	6250	1.9	744	13.9
108715	900	18.4	4500	2.7	1080	13.0
108716	900	18.4	4500	2.7	1125	13.0
108717	900	18.4	4500	2.7	1170	13.0
108718	900	18.4	4500	2.7	1215	13.0
108719	900	18.4	4500	2.7	1260	13.0
108720	900	18.4	4500	2.7	1305	13.0
108721	900	18.4	4500	2.7	1350	13.0
108722	900	18.4	4500	2.7	1395	13.0
108723	900	18.4	4500	2.7	1440	13.0
108724	900	18.4	4500	2.7	1485	13.0
108725	900	18.4	4500	2.7	1530	13.0
108726	900	18.4	4500	2.7	1575	13.0
108842	750	18.8	3750	3.1	1184	12.7
109182	500	16.7	2500	2.0	1925	12.7
108950	650	18.2	3250	2.9	1280	12.3
108754	850	17.3	4250	2.8	1134	11.7
108755	850	17.3	4250	2.8	1176	11.7
108756	850	17.3	4250	2.8	1218	11.7
108757	850	17.3	4250	2.8	1260	11.7
108758	850	17.3	4250	2.8	1302	11.7
108759	850	17.3	4250	2.8	1344	11.7
108760	850	17.3	4250	2.8	1386	11.7
108761	850	17.3	4250	2.8	1428	11.7
108762	850	17.3	4250	2.8	1470	11.7
108763	850	17.3	4250	2.8	1512	11.7
108764	850	17.3	4250	2.8	1554	11.7
108765	850	17.3	4250	2.8	1596	11.7
108766	850	17.3	4250	2.8	1638	11.7
109162	500	16.2	2500	2.8	1425	10.6
108796	800	16.3	4000	3.0	1160	10.3
108797	800	16.3	4000	3.0	1200	10.3
108798	800	16.3	4000	3.0	1240	10.3
108799	800	16.3	4000	3.0	1280	10.3
108800	800	16.3	4000	3.0	1320	10.3
108801	800	16.3	4000	3.0	1360	10.3
108802	800	16.3	4000	3.0	1400	10.3
108803	800	16.3	4000	3.0	1440	10.3
108804	800	16.3	4000	3.0	1480	10.3
108805	800	16.3	4000	3.0	1520	10.3
108806	800	16.3	4000	3.0	1560	10.3
108807	800	16.3	4000	3.0	1600	10.3
108808	800	16.3	4000	3.0	1640	10.3
108809	800	16.3	4000	3.0	1680	10.3
109083	550	15.8	2750	2.9	1377	10.0
109103	550	15.8	2750	3.3	1917	9.3
108843	750	15.3	3750	3.2	1221	8.9
108844	750	15.3	3750	3.2	1258	8.9
108845	750	15.3	3750	3.2	1295	8.9
108846	750	15.3	3750	3.2	1332	8.9
108847	750	15.3	3750	3.2	1369	8.9
108848	750	15.3	3750	3.2	1406	8.9
108849	750	15.3	3750	3.2	1443	8.9
108850	750	15.3	3750	3.2	1480	8.9
108851	750	15.3	3750	3.2	1517	8.9
108852	750	15.3	3750	3.2	1554	8.9
108853	750	15.3	3750	3.2	1591	8.9
108854	750	15.3	3750	3.2	1628	8.9
108855	750	15.3	3750	3.2	1665	8.9
108856	750	15.3	3750	3.2	1702	8.9
108857	750	15.3	3750	3.2	1739	8.9
109032	550	17.6	2750	4.5	0	8.5
108505	1250	16.4	6250	4.2	0	8.1
108510	1250	16.4	6250	4.2	310	8.1
108511	1250	16.4	6250	4.2	372	8.1
108512	1250	16.4	6250	4.2	434	8.1
108513	1250	16.4	6250	4.2	496	8.1
108894	700	14.3	3500	3.4	1260	7.4
108895	700	14.3	3500	3.4	1295	7.4
108896	700	14.3	3500	3.4	1330	7.4
108897	700	14.3	3500	3.4	1365	7.4
108898	700	14.3	3500	3.4	1400	7.4
108899	700	14.3	3500	3.4	1435	7.4
108900	700	14.3	3500	3.4	1470	7.4
108901	700	14.3	3500	3.4	1505	7.4
108902	700	14.3	3500	3.4	1540	7.4
108903	700	14.3	3500	3.4	1575	7.4
108904	700	14.3	3500	3.4	1610	7.4
108905	700	14.3	3500	3.4	1645	7.4
108906	700	14.3	3500	3.4	1680	7.4
108907	700	14.3	3500	3.4	1715	7.4
108908	700	14.3	3500	3.4	1750	7.4
108909	700	14.3	3500	3.4	1785	7.4
108526	1200	15.7	6000	4.3	0	7.1
108527	1200	15.7	6000	4.3	60	7.1
108528	1200	15.7	6000	4.3	120	7.1
108529	1200	15.7	6000	4.3	180	7.1
108530	1200	15.7	6000	4.3	240	7.1
108531	1200	15.7	6000	4.3	300	7.1
108532	1200	15.7	6000	4.3	360	7.1
108533	1200	15.7	6000	4.3	420	7.1
108534	1200	15.7	6000	4.3	480	7.1
109013	600	14.2	3000	4.0	1350	6.2
108548	1150	15.1	5750	4.5	0	6.0
108549	1150	15.1	5750	4.5	57	6.0
108550	1150	15.1	5750	4.5	114	6.0
108551	1150	15.1	5750	4.5	171	6.0
108552	1150	15.1	5750	4.5	228	6.0
108553	1150	15.1	5750	4.5	285	6.0
108554	1150	15.1	5750	4.5	342	6.0
108555	1150	15.1	5750	4.5	399	6.0
108556	1150	15.1	5750	4.5	456	6.0
108557	1150	15.1	5750	4.5	513	6.0
109107	500	16.0	2500	5.0	50	6.0
109031	600	14.0	3000	4.0	1890	6.0
108951	650	13.3	3250	3.7	1312	5.9
108952	650	13.3	3250	3.7	1344	5.9
108953	650	13.3	3250	3.7	1376	5.9
108954	650	13.3	3250	3.7	1408	5.9
108955	650	13.3	3250	3.7	1440	5.9
108956	650	13.3	3250	3.7	1472	5.9
108957	650	13.3	3250	3.7	1504	5.9
108958	650	13.3	3250	3.7	1536	5.9
108959	650	13.3	3250	3.7	1568	5.9
108960	650	13.3	3250	3.7	1600	5.9
108961	650	13.3	3250	3.7	1632	5.9
108962	650	13.3	3250	3.7	1664	5.9
108963	650	13.3	3250	3.7	1696	5.9
108964	650	13.3	3250	3.7	1728	5.9
108965	650	13.3	3250	3.7	1760	5.9
108966	650	13.3	3250	3.7	1792	5.9
108967	650	13.3	3250	3.7	1824	5.9
108572	1100	14.4	5500	4.7	0	5.0
108573	1100	14.4	5500	4.7	55	5.0
108574	1100	14.4	5500	4.7	110	5.0
108575	1100	14.4	5500	4.7	165	5.0
108576	1100	14.4	5500	4.7	220	5.0
108577	1100	14.4	5500	4.7	275	5.0
108578	1100	14.4	5500	4.7	330	5.0
108579	1100	14.4	5500	4.7	385	5.0
108580	1100	14.4	5500	4.7	440	5.0
108581	1100	14.4	5500	4.7	495	5.0
109014	600	12.2	3000	4.0	1380	4.2
109015	600	12.2	3000	4.0	1410	4.2
109016	600	12.2	3000	4.0	1440	4.2
109017	600	12.2	3000	4.0	1470	4.2
109018	600	12.2	3000	4.0	1500	4.2
109019	600	12.2	3000	4.0	1530	4.2
109020	600	12.2	3000	4.0	1560	4.2
109021	600	12.2	3000	4.0	1590	4.2
109022	600	12.2	3000	4.0	1620	4.2
109023	600	12.2	3000	4.0	1650	4.2
109024	600	12.2	3000	4.0	1680	4.2
109025	600	12.2	3000	4.0	1710	4.2
109026	600	12.2	3000	4.0	1740	4.2
109027	600	12.2	3000	4.0	1770	4.2
109028	600	12.2	3000	4.0	1800	4.2
109029	600	12.2	3000	4.0	1830	4.2
109030	600	12.2	3000	4.0	1860	4.2
109102	550	12.9	2750	4.4	1890	4.1
108598	1050	13.8	5250	5.0	0	3.9
108599	1050	13.8	5250	5.0	52	3.9
108600	1050	13.8	5250	5.0	104	3.9
108601	1050	13.8	5250	5.0	156	3.9
108602	1050	13.8	5250	5.0	208	3.9
108603	1050	13.8	5250	5.0	260	3.9
108604	1050	13.8	5250	5.0	312	3.9
108605	1050	13.8	5250	5.0	364	3.9
108606	1050	13.8	5250	5.0	416	3.9
108607	1050	13.8	5250	5.0	468	3.9
108608	1050	13.8	5250	5.0	520	3.9
108703	900	11.8	4500	4.1	540	3.6
109181	500	12.3	2500	4.8	1900	2.7
109084	550	11.2	2750	4.4	1404	2.5
109085	550	11.2	2750	4.4	1431	2.5
109086	550	11.2	2750	4.4	1458	2.5
109087	550	11.2	2750	4.4	1485	2.5
109088	550	11.2	2750	4.4	1512	2.5
109089	550	11.2	2750	4.4	1539	2.5
109090	550	11.2	2750	4.4	1566	2.5
109091	550	11.2	2750	4.4	1593	2.5
109092	550	11.2	2750	4.4	1620	2.5
109093	550	11.2	2750	4.4	1647	2.5
109094	550	11.2	2750	4.4	1674	2.5
109095	550	11.2	2750	4.4	1701	2.5
109096	550	11.2	2750	4.4	1728	2.5
109097	550	11.2	2750	4.4	1755	2.5
109098	550	11.2	2750	4.4	1782	2.5
109099	550	11.2	2750	4.4	1809	2.5
109100	550	11.2	2750	4.4	1836	2.5
109101	550	11.2	2750	4.4	1863	2.5
109163	500	11.8	2500	4.8	1450	2.2
109164	500	10.2	2500	4.8	1475	0.6
109165	500	10.2	2500	4.8	1500	0.6
109166	500	10.2	2500	4.8	1525	0.6
109167	500	10.2	2500	4.8	1550	0.6
109168	500	10.2	2500	4.8	1575	0.6
109169	500	10.2	2500	4.8	1600	0.6
109170	500	10.2	2500	4.8	1625	0.6
109171	500	10.2	2500	4.8	1650	0.6
109172	500	10.2	2500	4.8	1675	0.6
109173	500	10.2	2500	4.8	1700	0.6
109174	500	10.2	2500	4.8	1725	0.6
109175	500	10.2	2500	4.8	1750	0.6
109176	500	10.2	2500	4.8	1775	0.6
109177	500	10.2	2500	4.8	1800	0.6
109178	500	10.2	2500	4.8	1825	0.6
109179	500	10.2	2500	4.8	1850	0.6
109180	500	10.2	2500	4.8	1875	0.6

Overall score = score_ratio - 2 * repetitive DNA content