Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign13 - back to list

Summary
Image
Unique putative probes (109)
Putative probes (448)
Redundant probes (263)

Summary

`./analyse_probe_search_results : Fri Feb 16 09:54:30 2007`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign13
  assembly      : NCBIM36
  bias          : 5prime
  chromosome    : 16
  id            : 7
  strand        : 1
  -------------

Design window length: 3964bp

All jobs for probe design are successful - total: 66
Fetched conf : Exonerate mouse_NCBIM36 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 1305 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1305/1305
Putative probes that are unique                 : 109/1305
Putative probes exceeding selection criteria    : 820/1305 (both unique and not unique)
Putative probes with successfully-picked primers: 501/820

Identified 263 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
6023	900	4500	-	1485
6094	850	4250	-	1470
6095	850	4250	-	1512
6171	800	4000	-	1480
6172	800	4000	-	1520
6173	800	4000	-	1560
6254	750	3750	-	1480
6255	750	3750	-	1517
6256	750	3750	-	1554
6257	750	3750	-	1591
6258	750	3750	-	1628
6296	750	3750	-	3034
6343	700	3500	-	1470
6344	700	3500	-	1505
6345	700	3500	-	1540
6346	700	3500	-	1575
6347	700	3500	-	1610
6348	700	3500	-	1645
6349	700	3500	-	1680
6388	700	3500	-	3045
6389	700	3500	-	3080
6441	650	3250	-	1472
6442	650	3250	-	1504
6443	650	3250	-	1536
6444	650	3250	-	1568
6445	650	3250	-	1600
6446	650	3250	-	1632
6447	650	3250	-	1664
6448	650	3250	-	1696
6449	650	3250	-	1728
6490	650	3250	-	3040
6491	650	3250	-	3072
6492	650	3250	-	3104
6493	650	3250	-	3136
6548	600	3000	-	1470
6549	600	3000	-	1500
6550	600	3000	-	1530
6551	600	3000	-	1560
6552	600	3000	-	1590
6553	600	3000	-	1620
6554	600	3000	-	1650
6555	600	3000	-	1680
6556	600	3000	-	1710
6557	600	3000	-	1740
6558	600	3000	-	1770
6600	600	3000	-	3030
6601	600	3000	-	3060
6602	600	3000	-	3090
6603	600	3000	-	3120
6604	600	3000	-	3150
6605	600	3000	-	3180
6666	550	2750	-	1458
6667	550	2750	-	1485
6668	550	2750	-	1512
6669	550	2750	-	1539
6670	550	2750	-	1566
6671	550	2750	-	1593
6672	550	2750	-	1620
6673	550	2750	-	1647
6674	550	2750	-	1674
6675	550	2750	-	1701
6676	550	2750	-	1728
6677	550	2750	-	1755
6678	550	2750	-	1782
6679	550	2750	-	1809
6680	550	2750	-	1836
6724	550	2750	-	3024
6725	550	2750	-	3051
6726	550	2750	-	3078
6727	550	2750	-	3105
6728	550	2750	-	3132
6729	550	2750	-	3159
6730	550	2750	-	3186
6731	550	2750	-	3213
6732	550	2750	-	3240
6798	500	2500	-	1475
6799	500	2500	-	1500
6800	500	2500	-	1525
6801	500	2500	-	1550
6802	500	2500	-	1575
6803	500	2500	-	1600
6804	500	2500	-	1625
6805	500	2500	-	1650
6806	500	2500	-	1675
6807	500	2500	-	1700
6808	500	2500	-	1725
6809	500	2500	-	1750
6810	500	2500	-	1775
6811	500	2500	-	1800
6812	500	2500	-	1825
6813	500	2500	-	1850
6814	500	2500	-	1875
6860	500	2500	-	3025
6861	500	2500	-	3050
6862	500	2500	-	3075
6863	500	2500	-	3100
6864	500	2500	-	3125
6865	500	2500	-	3150
6866	500	2500	-	3175
6867	500	2500	-	3200
6868	500	2500	-	3225
6869	500	2500	-	3250
6870	500	2500	-	3275
6387	700	3500	0.1	3010
6489	650	3250	0.5	3008
6599	600	3000	1.8	3000
6295	750	3750	1.9	2997
6859	500	2500	2.2	3000
6723	550	2750	2.5	2997

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
5590	1300	41.4	6500	-	1105	41.4
5591	1300	41.4	6500	-	1170	41.4
5592	1300	41.4	6500	-	1235	41.4
5593	1300	41.4	6500	-	1300	41.4
5594	1300	41.4	6500	-	1365	41.4
5595	1300	41.4	6500	-	1430	41.4
5638	1250	41.4	6250	-	1488	41.4
5596	1300	41.1	6500	-	1495	41.1
5597	1300	41.1	6500	-	1560	41.1
5598	1300	41.1	6500	-	1625	41.1
5599	1300	41.1	6500	0.6	1690	39.9
5632	1250	39.8	6250	-	1116	39.8
5633	1250	39.8	6250	-	1178	39.8
5634	1250	39.8	6250	-	1240	39.8
5635	1250	39.8	6250	-	1302	39.8
5636	1250	39.8	6250	-	1364	39.8
5637	1250	39.8	6250	-	1426	39.8
5683	1200	39.7	6000	-	1500	39.7
5684	1200	39.7	6000	-	1560	39.7
5639	1250	39.6	6250	-	1550	39.6
5640	1250	39.6	6250	-	1612	39.6
5641	1250	39.6	6250	-	1674	39.6
5642	1250	39.6	6250	0.3	1736	38.9
5677	1200	38.2	6000	-	1140	38.2
5678	1200	38.2	6000	-	1200	38.2
5679	1200	38.2	6000	-	1260	38.2
5680	1200	38.2	6000	-	1320	38.2
5681	1200	38.2	6000	-	1380	38.2
5682	1200	38.2	6000	-	1440	38.2
5731	1150	38.1	5750	-	1482	38.1
5732	1150	38.1	5750	-	1539	38.1
5733	1150	38.1	5750	-	1596	38.1
5685	1200	38.0	6000	-	1620	38.0
5686	1200	38.0	6000	-	1680	38.0
5687	1200	38.0	6000	-	1740	38.0
5725	1150	36.6	5750	-	1140	36.6
5726	1150	36.6	5750	-	1197	36.6
5727	1150	36.6	5750	-	1254	36.6
5728	1150	36.6	5750	-	1311	36.6
5729	1150	36.6	5750	-	1368	36.6
5730	1150	36.6	5750	-	1425	36.6
5782	1100	36.4	5500	-	1485	36.4
5783	1100	36.4	5500	-	1540	36.4
5784	1100	36.4	5500	-	1595	36.4
5785	1100	36.4	5500	-	1650	36.4
5734	1150	36.4	5750	-	1653	36.4
5735	1150	36.4	5750	-	1710	36.4
5736	1150	36.4	5750	-	1767	36.4
5737	1150	36.4	5750	-	1824	36.4
5776	1100	35.0	5500	-	1155	35.0
5777	1100	35.0	5500	-	1210	35.0
5778	1100	35.0	5500	-	1265	35.0
5779	1100	35.0	5500	-	1320	35.0
5780	1100	35.0	5500	-	1375	35.0
5781	1100	35.0	5500	-	1430	35.0
5688	1200	38.0	6000	1.5	1800	35.0
5786	1100	34.8	5500	-	1705	34.8
5787	1100	34.8	5500	-	1760	34.8
5788	1100	34.8	5500	-	1815	34.8
5789	1100	34.8	5500	-	1870	34.8
5836	1050	34.8	5250	-	1456	34.8
5837	1050	34.8	5250	-	1508	34.8
5838	1050	34.8	5250	-	1560	34.8
5839	1050	34.8	5250	-	1612	34.8
5840	1050	34.8	5250	-	1664	34.8
5841	1050	34.8	5250	-	1716	34.8
5775	1100	35.0	5500	0.3	1100	34.5
5676	1200	38.2	6000	1.9	1080	34.4
5830	1050	33.4	5250	-	1144	33.4
5831	1050	33.4	5250	-	1196	33.4
5832	1050	33.4	5250	-	1248	33.4
5833	1050	33.4	5250	-	1300	33.4
5834	1050	33.4	5250	-	1352	33.4
5835	1050	33.4	5250	-	1404	33.4
5842	1050	33.2	5250	-	1768	33.2
5843	1050	33.2	5250	-	1820	33.2
5844	1050	33.2	5250	-	1872	33.2
5845	1050	33.2	5250	-	1924	33.2
5724	1150	36.6	5750	1.7	1083	33.1
5894	1000	33.1	5000	-	1450	33.1
5895	1000	33.1	5000	-	1500	33.1
5896	1000	33.1	5000	-	1550	33.1
5897	1000	33.1	5000	-	1600	33.1
5898	1000	33.1	5000	-	1650	33.1
5899	1000	33.1	5000	-	1700	33.1
5900	1000	33.1	5000	-	1750	33.1
5600	1300	36.7	6500	2.2	1755	32.3
5601	1300	36.5	6500	2.2	1820	32.1
5602	1300	36.5	6500	2.2	1885	32.1
5603	1300	36.5	6500	2.2	1950	32.1
5604	1300	36.5	6500	2.2	2015	32.1
5605	1300	36.5	6500	2.2	2080	32.1
5606	1300	36.5	6500	2.2	2145	32.1
5607	1300	36.5	6500	2.2	2210	32.1
5608	1300	36.5	6500	2.2	2275	32.1
5609	1300	36.5	6500	2.2	2340	32.1
5610	1300	36.5	6500	2.2	2405	32.1
5611	1300	36.5	6500	2.2	2470	32.1
5612	1300	36.5	6500	2.2	2535	32.1
5613	1300	36.5	6500	2.2	2600	32.1
5631	1250	39.8	6250	3.9	1054	32.0
5888	1000	31.8	5000	-	1150	31.8
5889	1000	31.8	5000	-	1200	31.8
5890	1000	31.8	5000	-	1250	31.8
5891	1000	31.8	5000	-	1300	31.8
5892	1000	31.8	5000	-	1350	31.8
5893	1000	31.8	5000	-	1400	31.8
5901	1000	31.6	5000	-	1800	31.6
5902	1000	31.6	5000	-	1850	31.6
5903	1000	31.6	5000	-	1900	31.6
5904	1000	31.6	5000	-	1950	31.6
5956	950	31.5	4750	-	1457	31.5
5957	950	31.5	4750	-	1504	31.5
5958	950	31.5	4750	-	1551	31.5
5959	950	31.5	4750	-	1598	31.5
5960	950	31.5	4750	-	1645	31.5
5961	950	31.5	4750	-	1692	31.5
5962	950	31.5	4750	-	1739	31.5
5963	950	31.5	4750	-	1786	31.5
5964	950	31.5	4750	-	1833	31.5
5829	1050	33.4	5250	1.0	1092	31.3
5887	1000	31.8	5000	0.3	1100	31.2
5657	1250	35.1	6250	2.3	2666	30.5
5989	950	31.0	4750	0.3	3008	30.4
5949	950	30.3	4750	-	1128	30.3
5950	950	30.3	4750	-	1175	30.3
5951	950	30.3	4750	-	1222	30.3
5952	950	30.3	4750	-	1269	30.3
5953	950	30.3	4750	-	1316	30.3
5954	950	30.3	4750	-	1363	30.3
5955	950	30.3	4750	-	1410	30.3
5965	950	30.1	4750	-	1880	30.1
5966	950	30.1	4750	-	1927	30.1
5967	950	30.1	4750	-	1974	30.1
5968	950	30.1	4750	-	2021	30.1
6024	900	29.8	4500	-	1530	29.8
6025	900	29.8	4500	-	1575	29.8
6026	900	29.8	4500	-	1620	29.8
6027	900	29.8	4500	-	1665	29.8
6028	900	29.8	4500	-	1710	29.8
6029	900	29.8	4500	-	1755	29.8
6030	900	29.8	4500	-	1800	29.8
6031	900	29.8	4500	-	1845	29.8
6057	900	29.4	4500	-	3015	29.4
6058	900	29.4	4500	-	3060	29.4
5704	1200	33.7	6000	2.4	2760	28.9
6015	900	28.7	4500	-	1125	28.7
6016	900	28.7	4500	-	1170	28.7
6017	900	28.7	4500	-	1215	28.7
6018	900	28.7	4500	-	1260	28.7
6019	900	28.7	4500	-	1305	28.7
6020	900	28.7	4500	-	1350	28.7
6021	900	28.7	4500	-	1395	28.7
6022	900	28.7	4500	-	1440	28.7
5589	1300	38.2	6500	4.8	1040	28.5
6032	900	28.5	4500	-	1890	28.5
6033	900	28.5	4500	-	1935	28.5
6034	900	28.5	4500	-	1980	28.5
6035	900	28.5	4500	-	2025	28.5
6036	900	28.5	4500	-	2070	28.5
6096	850	28.1	4250	-	1554	28.1
6097	850	28.1	4250	-	1596	28.1
6098	850	28.1	4250	-	1638	28.1
6099	850	28.1	4250	-	1680	28.1
6100	850	28.1	4250	-	1722	28.1
6101	850	28.1	4250	-	1764	28.1
6102	850	28.1	4250	-	1806	28.1
6103	850	28.1	4250	-	1848	28.1
6104	850	28.1	4250	-	1890	28.1
6105	850	28.1	4250	-	1932	28.1
5905	1000	31.6	5000	1.8	2000	28.0
6131	850	27.8	4250	-	3024	27.8
6132	850	27.8	4250	-	3066	27.8
6133	850	27.8	4250	-	3108	27.8
6086	850	27.1	4250	-	1134	27.1
6087	850	27.1	4250	-	1176	27.1
6088	850	27.1	4250	-	1218	27.1
6089	850	27.1	4250	-	1260	27.1
6090	850	27.1	4250	-	1302	27.1
6091	850	27.1	4250	-	1344	27.1
6092	850	27.1	4250	-	1386	27.1
6093	850	27.1	4250	-	1428	27.1
6106	850	26.9	4250	-	1974	26.9
6107	850	26.9	4250	-	2016	26.9
6108	850	26.9	4250	-	2058	26.9
6109	850	26.9	4250	-	2100	26.9
6174	800	26.5	4000	-	1600	26.5
6175	800	26.5	4000	-	1640	26.5
6176	800	26.5	4000	-	1680	26.5
6177	800	26.5	4000	-	1720	26.5
6178	800	26.5	4000	-	1760	26.5
6179	800	26.5	4000	-	1800	26.5
6180	800	26.5	4000	-	1840	26.5
6181	800	26.5	4000	-	1880	26.5
6182	800	26.5	4000	-	1920	26.5
6183	800	26.5	4000	-	1960	26.5
6210	800	26.1	4000	-	3040	26.1
6211	800	26.1	4000	-	3080	26.1
6212	800	26.1	4000	-	3120	26.1
6213	800	26.1	4000	-	3160	26.1
5948	950	30.3	4750	2.3	1081	25.6
6162	800	25.5	4000	-	1120	25.5
6163	800	25.5	4000	-	1160	25.5
6164	800	25.5	4000	-	1200	25.5
6165	800	25.5	4000	-	1240	25.5
6166	800	25.5	4000	-	1280	25.5
6167	800	25.5	4000	-	1320	25.5
6168	800	25.5	4000	-	1360	25.5
6169	800	25.5	4000	-	1400	25.5
6170	800	25.5	4000	-	1440	25.5
6184	800	25.3	4000	-	2000	25.3
6185	800	25.3	4000	-	2040	25.3
6186	800	25.3	4000	-	2080	25.3
6187	800	25.3	4000	-	2120	25.3
6188	800	25.3	4000	-	2160	25.3
6259	750	24.8	3750	-	1665	24.8
6260	750	24.8	3750	-	1702	24.8
6261	750	24.8	3750	-	1739	24.8
6262	750	24.8	3750	-	1776	24.8
6263	750	24.8	3750	-	1813	24.8
6264	750	24.8	3750	-	1850	24.8
6265	750	24.8	3750	-	1887	24.8
6266	750	24.8	3750	-	1924	24.8
6267	750	24.8	3750	-	1961	24.8
6268	750	24.8	3750	-	1998	24.8
6269	750	24.7	3750	-	2035	24.7
6110	850	26.9	4250	1.2	2142	24.5
6297	750	24.5	3750	-	3071	24.5
6298	750	24.5	3750	-	3108	24.5
6299	750	24.5	3750	-	3145	24.5
6300	750	24.5	3750	-	3182	24.5
6085	850	27.1	4250	1.3	1092	24.5
5864	1050	29.5	5250	2.8	2912	24.0
6244	750	23.9	3750	-	1110	23.9
6245	750	23.9	3750	-	1147	23.9
6246	750	23.9	3750	-	1184	23.9
6247	750	23.9	3750	-	1221	23.9
6248	750	23.9	3750	-	1258	23.9
6249	750	23.9	3750	-	1295	23.9
6250	750	23.9	3750	-	1332	23.9
6251	750	23.9	3750	-	1369	23.9
6252	750	23.9	3750	-	1406	23.9
6253	750	23.9	3750	-	1443	23.9
6270	750	23.7	3750	-	2072	23.7
6271	750	23.7	3750	-	2109	23.7
6272	750	23.7	3750	-	2146	23.7
6273	750	23.7	3750	-	2183	23.7
6274	750	23.7	3750	-	2220	23.7
6014	900	28.7	4500	2.6	1080	23.6
6209	800	26.1	4000	1.4	3000	23.4
6350	700	23.2	3500	-	1715	23.2
6351	700	23.2	3500	-	1750	23.2
6352	700	23.2	3500	-	1785	23.2
6353	700	23.2	3500	-	1820	23.2
6354	700	23.2	3500	-	1855	23.2
6355	700	23.2	3500	-	1890	23.2
6356	700	23.2	3500	-	1925	23.2
6357	700	23.2	3500	-	1960	23.2
6358	700	23.2	3500	-	1995	23.2
6359	700	23.2	3500	-	2030	23.2
6360	700	23.2	3500	-	2065	23.2
6390	700	22.9	3500	-	3115	22.9
6391	700	22.9	3500	-	3150	22.9
6392	700	22.9	3500	-	3185	22.9
6393	700	22.9	3500	-	3220	22.9
6394	700	22.9	3500	-	3255	22.9
6333	700	22.3	3500	-	1120	22.3
6334	700	22.3	3500	-	1155	22.3
6335	700	22.3	3500	-	1190	22.3
6336	700	22.3	3500	-	1225	22.3
6337	700	22.3	3500	-	1260	22.3
6338	700	22.3	3500	-	1295	22.3
6339	700	22.3	3500	-	1330	22.3
6340	700	22.3	3500	-	1365	22.3
6341	700	22.3	3500	-	1400	22.3
6342	700	22.3	3500	-	1435	22.3
6361	700	22.2	3500	-	2100	22.2
6362	700	22.2	3500	-	2135	22.2
6363	700	22.2	3500	-	2170	22.2
6364	700	22.2	3500	-	2205	22.2
6365	700	22.2	3500	-	2240	22.2
6366	700	22.2	3500	-	2275	22.2
6450	650	21.5	3250	-	1760	21.5
6451	650	21.5	3250	-	1792	21.5
6452	650	21.5	3250	-	1824	21.5
6453	650	21.5	3250	-	1856	21.5
6454	650	21.5	3250	-	1888	21.5
6455	650	21.5	3250	-	1920	21.5
6456	650	21.5	3250	-	1952	21.5
6457	650	21.5	3250	-	1984	21.5
6458	650	21.5	3250	-	2016	21.5
6459	650	21.5	3250	-	2048	21.5
6460	650	21.5	3250	-	2080	21.5
6461	650	21.5	3250	-	2112	21.5
6494	650	21.2	3250	-	3168	21.2
6495	650	21.2	3250	-	3200	21.2
6496	650	21.2	3250	-	3232	21.2
6497	650	21.2	3250	-	3264	21.2
6498	650	21.2	3250	-	3296	21.2
6430	650	20.7	3250	-	1120	20.7
6431	650	20.7	3250	-	1152	20.7
6432	650	20.7	3250	-	1184	20.7
6433	650	20.7	3250	-	1216	20.7
6434	650	20.7	3250	-	1248	20.7
6435	650	20.7	3250	-	1280	20.7
6436	650	20.7	3250	-	1312	20.7
6437	650	20.7	3250	-	1344	20.7
6438	650	20.7	3250	-	1376	20.7
6439	650	20.7	3250	-	1408	20.7
6440	650	20.7	3250	-	1440	20.7
6462	650	20.6	3250	-	2144	20.6
6463	650	20.6	3250	-	2176	20.6
6464	650	20.6	3250	-	2208	20.6
6465	650	20.6	3250	-	2240	20.6
6466	650	20.6	3250	-	2272	20.6
6467	650	20.6	3250	-	2304	20.6
6559	600	19.9	3000	-	1800	19.9
6560	600	19.9	3000	-	1830	19.9
6561	600	19.9	3000	-	1860	19.9
6562	600	19.9	3000	-	1890	19.9
6563	600	19.9	3000	-	1920	19.9
6564	600	19.9	3000	-	1950	19.9
6565	600	19.9	3000	-	1980	19.9
6566	600	19.9	3000	-	2010	19.9
6567	600	19.9	3000	-	2040	19.9
6568	600	19.9	3000	-	2070	19.9
6569	600	19.9	3000	-	2100	19.9
6570	600	19.9	3000	-	2130	19.9
6571	600	19.9	3000	-	2160	19.9
6161	800	25.5	4000	2.9	1080	19.7
6606	600	19.6	3000	-	3210	19.6
6607	600	19.6	3000	-	3240	19.6
6608	600	19.6	3000	-	3270	19.6
6609	600	19.6	3000	-	3300	19.6
6610	600	19.6	3000	-	3330	19.6
6611	600	19.6	3000	-	3360	19.6
6468	650	20.6	3250	0.6	2336	19.3
6536	600	19.1	3000	-	1110	19.1
6537	600	19.1	3000	-	1140	19.1
6538	600	19.1	3000	-	1170	19.1
6539	600	19.1	3000	-	1200	19.1
6540	600	19.1	3000	-	1230	19.1
6541	600	19.1	3000	-	1260	19.1
6542	600	19.1	3000	-	1290	19.1
6543	600	19.1	3000	-	1320	19.1
6544	600	19.1	3000	-	1350	19.1
6545	600	19.1	3000	-	1380	19.1
6546	600	19.1	3000	-	1410	19.1
6547	600	19.1	3000	-	1440	19.1
6572	600	19.0	3000	-	2190	19.0
6573	600	19.0	3000	-	2220	19.0
6574	600	19.0	3000	-	2250	19.0
6575	600	19.0	3000	-	2280	19.0
6576	600	19.0	3000	-	2310	19.0
6577	600	19.0	3000	-	2340	19.0
6578	600	19.0	3000	-	2370	19.0
6681	550	18.2	2750	-	1863	18.2
6682	550	18.2	2750	-	1890	18.2
6683	550	18.2	2750	-	1917	18.2
6684	550	18.2	2750	-	1944	18.2
6685	550	18.2	2750	-	1971	18.2
6686	550	18.2	2750	-	1998	18.2
6687	550	18.2	2750	-	2025	18.2
6688	550	18.2	2750	-	2052	18.2
6689	550	18.2	2750	-	2079	18.2
6690	550	18.2	2750	-	2106	18.2
6691	550	18.2	2750	-	2133	18.2
6692	550	18.2	2750	-	2160	18.2
6693	550	18.2	2750	-	2187	18.2
6694	550	18.2	2750	-	2214	18.2
6733	550	18.0	2750	-	3267	18.0
6734	550	18.0	2750	-	3294	18.0
6735	550	18.0	2750	-	3321	18.0
6736	550	18.0	2750	-	3348	18.0
6737	550	18.0	2750	-	3375	18.0
6738	550	18.0	2750	-	3402	18.0
6653	550	17.5	2750	-	1107	17.5
6654	550	17.5	2750	-	1134	17.5
6655	550	17.5	2750	-	1161	17.5
6656	550	17.5	2750	-	1188	17.5
6657	550	17.5	2750	-	1215	17.5
6658	550	17.5	2750	-	1242	17.5
6659	550	17.5	2750	-	1269	17.5
6660	550	17.5	2750	-	1296	17.5
6661	550	17.5	2750	-	1323	17.5
6662	550	17.5	2750	-	1350	17.5
6663	550	17.5	2750	-	1377	17.5
6664	550	17.5	2750	-	1404	17.5
6665	550	17.5	2750	-	1431	17.5
6695	550	17.4	2750	-	2241	17.4
6696	550	17.4	2750	-	2268	17.4
6697	550	17.4	2750	-	2295	17.4
6698	550	17.4	2750	-	2322	17.4
6699	550	17.4	2750	-	2349	17.4
6700	550	17.4	2750	-	2376	17.4
6701	550	17.4	2750	-	2403	17.4
6702	550	17.4	2750	-	2430	17.4
6332	700	22.3	3500	2.6	1085	17.2
6797	500	16.7	2500	-	1450	16.7
6815	500	16.6	2500	-	1900	16.6
6816	500	16.6	2500	-	1925	16.6
6817	500	16.6	2500	-	1950	16.6
6818	500	16.6	2500	-	1975	16.6
6819	500	16.6	2500	-	2000	16.6
6820	500	16.6	2500	-	2025	16.6
6821	500	16.6	2500	-	2050	16.6
6822	500	16.6	2500	-	2075	16.6
6823	500	16.6	2500	-	2100	16.6
6824	500	16.6	2500	-	2125	16.6
6825	500	16.6	2500	-	2150	16.6
6826	500	16.6	2500	-	2175	16.6
6827	500	16.6	2500	-	2200	16.6
6828	500	16.6	2500	-	2225	16.6
6829	500	16.6	2500	-	2250	16.6
6830	500	16.6	2500	-	2275	16.6
6871	500	16.3	2500	-	3300	16.3
6872	500	16.3	2500	-	3325	16.3
6873	500	16.3	2500	-	3350	16.3
6874	500	16.3	2500	-	3375	16.3
6875	500	16.3	2500	-	3400	16.3
6876	500	16.3	2500	-	3425	16.3
6877	500	16.3	2500	-	3450	16.3
6429	650	20.7	3250	2.3	1088	16.1
6784	500	15.9	2500	-	1125	15.9
6785	500	15.9	2500	-	1150	15.9
6786	500	15.9	2500	-	1175	15.9
6787	500	15.9	2500	-	1200	15.9
6788	500	15.9	2500	-	1225	15.9
6789	500	15.9	2500	-	1250	15.9
6790	500	15.9	2500	-	1275	15.9
6791	500	15.9	2500	-	1300	15.9
6792	500	15.9	2500	-	1325	15.9
6793	500	15.9	2500	-	1350	15.9
6794	500	15.9	2500	-	1375	15.9
6795	500	15.9	2500	-	1400	15.9
6796	500	15.9	2500	-	1425	15.9
6243	750	23.9	3750	4.0	1073	15.9
6831	500	15.8	2500	-	2300	15.8
6832	500	15.8	2500	-	2325	15.8
6833	500	15.8	2500	-	2350	15.8
6834	500	15.8	2500	-	2375	15.8
6835	500	15.8	2500	-	2400	15.8
6836	500	15.8	2500	-	2425	15.8
6837	500	15.8	2500	-	2450	15.8
6838	500	15.8	2500	-	2475	15.8
6783	500	15.9	2500	0.6	1100	14.7
6535	600	19.1	3000	3.8	1080	11.4
6652	550	17.5	2750	4.2	1080	9.2

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
5643	1250	35.9	6250	2.3	1798	31.3
5644	1250	35.1	6250	2.3	1860	30.5
5645	1250	35.1	6250	2.3	1922	30.5
5646	1250	35.1	6250	2.3	1984	30.5
5647	1250	35.1	6250	2.3	2046	30.5
5648	1250	35.1	6250	2.3	2108	30.5
5649	1250	35.1	6250	2.3	2170	30.5
5650	1250	35.1	6250	2.3	2232	30.5
5651	1250	35.1	6250	2.3	2294	30.5
5652	1250	35.1	6250	2.3	2356	30.5
5653	1250	35.1	6250	2.3	2418	30.5
5654	1250	35.1	6250	2.3	2480	30.5
5655	1250	35.1	6250	2.3	2542	30.5
5656	1250	35.1	6250	2.3	2604	30.5
5738	1150	35.3	5750	2.5	1881	30.2
5689	1200	33.7	6000	2.4	1860	28.9
5690	1200	33.7	6000	2.4	1920	28.9
5691	1200	33.7	6000	2.4	1980	28.9
5692	1200	33.7	6000	2.4	2040	28.9
5693	1200	33.7	6000	2.4	2100	28.9
5694	1200	33.7	6000	2.4	2160	28.9
5695	1200	33.7	6000	2.4	2220	28.9
5696	1200	33.7	6000	2.4	2280	28.9
5697	1200	33.7	6000	2.4	2340	28.9
5698	1200	33.7	6000	2.4	2400	28.9
5699	1200	33.7	6000	2.4	2460	28.9
5700	1200	33.7	6000	2.4	2520	28.9
5701	1200	33.7	6000	2.4	2580	28.9
5702	1200	33.7	6000	2.4	2640	28.9
5703	1200	33.7	6000	2.4	2700	28.9
5790	1100	33.7	5500	2.6	1925	28.5
5739	1150	32.3	5750	2.5	1938	27.3
5740	1150	32.3	5750	2.5	1995	27.3
5741	1150	32.3	5750	2.5	2052	27.3
5742	1150	32.3	5750	2.5	2109	27.3
5743	1150	32.3	5750	2.5	2166	27.3
5744	1150	32.3	5750	2.5	2223	27.3
5745	1150	32.3	5750	2.5	2280	27.3
5746	1150	32.3	5750	2.5	2337	27.3
5747	1150	32.3	5750	2.5	2394	27.3
5748	1150	32.3	5750	2.5	2451	27.3
5749	1150	32.3	5750	2.5	2508	27.3
5750	1150	32.3	5750	2.5	2565	27.3
5751	1150	32.3	5750	2.5	2622	27.3
5752	1150	32.3	5750	2.5	2679	27.3
5753	1150	32.3	5750	2.5	2736	27.3
5754	1150	32.3	5750	2.5	2793	27.3
5846	1050	32.0	5250	2.8	1976	26.5
5791	1100	30.9	5500	2.6	1980	25.6
5792	1100	30.9	5500	2.6	2035	25.6
5793	1100	30.9	5500	2.6	2090	25.6
5794	1100	30.9	5500	2.6	2145	25.6
5795	1100	30.9	5500	2.6	2200	25.6
5796	1100	30.9	5500	2.6	2255	25.6
5797	1100	30.9	5500	2.6	2310	25.6
5798	1100	30.9	5500	2.6	2365	25.6
5799	1100	30.9	5500	2.6	2420	25.6
5800	1100	30.9	5500	2.6	2475	25.6
5801	1100	30.9	5500	2.6	2530	25.6
5802	1100	30.9	5500	2.6	2585	25.6
5803	1100	30.9	5500	2.6	2640	25.6
5804	1100	30.9	5500	2.6	2695	25.6
5805	1100	30.9	5500	2.6	2750	25.6
5806	1100	30.9	5500	2.6	2805	25.6
5807	1100	30.9	5500	2.6	2860	25.6
5847	1050	29.5	5250	2.8	2028	24.0
5848	1050	29.5	5250	2.8	2080	24.0
5849	1050	29.5	5250	2.8	2132	24.0
5850	1050	29.5	5250	2.8	2184	24.0
5851	1050	29.5	5250	2.8	2236	24.0
5852	1050	29.5	5250	2.8	2288	24.0
5853	1050	29.5	5250	2.8	2340	24.0
5854	1050	29.5	5250	2.8	2392	24.0
5855	1050	29.5	5250	2.8	2444	24.0
5856	1050	29.5	5250	2.8	2496	24.0
5857	1050	29.5	5250	2.8	2548	24.0
5858	1050	29.5	5250	2.8	2600	24.0
5859	1050	29.5	5250	2.8	2652	24.0
5860	1050	29.5	5250	2.8	2704	24.0
5861	1050	29.5	5250	2.8	2756	24.0
5862	1050	29.5	5250	2.8	2808	24.0
5863	1050	29.5	5250	2.8	2860	24.0
5969	950	29.7	4750	3.1	2068	23.6
5906	1000	28.6	5000	2.9	2050	22.8
5907	1000	28.1	5000	2.9	2100	22.3
5908	1000	28.1	5000	2.9	2150	22.3
5909	1000	28.1	5000	2.9	2200	22.3
5910	1000	28.1	5000	2.9	2250	22.3
5911	1000	28.1	5000	2.9	2300	22.3
5912	1000	28.1	5000	2.9	2350	22.3
5913	1000	28.1	5000	2.9	2400	22.3
5914	1000	28.1	5000	2.9	2450	22.3
5915	1000	28.1	5000	2.9	2500	22.3
5916	1000	28.1	5000	2.9	2550	22.3
5917	1000	28.1	5000	2.9	2600	22.3
5918	1000	28.1	5000	2.9	2650	22.3
5919	1000	28.1	5000	2.9	2700	22.3
5920	1000	28.1	5000	2.9	2750	22.3
5921	1000	28.1	5000	2.9	2800	22.3
5922	1000	28.1	5000	2.9	2850	22.3
5923	1000	28.1	5000	2.9	2900	22.3
5924	1000	28.1	5000	2.9	2950	22.3
6037	900	27.8	4500	3.2	2115	21.3
6189	800	25.3	4000	2.2	2200	20.8
5970	950	26.7	4750	3.1	2115	20.6
5971	950	26.7	4750	3.1	2162	20.6
5972	950	26.7	4750	3.1	2209	20.6
5973	950	26.7	4750	3.1	2256	20.6
5974	950	26.7	4750	3.1	2303	20.6
5975	950	26.7	4750	3.1	2350	20.6
5976	950	26.7	4750	3.1	2397	20.6
5977	950	26.7	4750	3.1	2444	20.6
5978	950	26.7	4750	3.1	2491	20.6
5979	950	26.7	4750	3.1	2538	20.6
5980	950	26.7	4750	3.1	2585	20.6
5981	950	26.7	4750	3.1	2632	20.6
5982	950	26.7	4750	3.1	2679	20.6
5983	950	26.7	4750	3.1	2726	20.6
5984	950	26.7	4750	3.1	2773	20.6
5985	950	26.7	4750	3.1	2820	20.6
5986	950	26.7	4750	3.1	2867	20.6
5987	950	26.7	4750	3.1	2914	20.6
5988	950	26.7	4750	3.1	2961	20.6
6111	850	26.1	4250	3.4	2184	19.3
6038	900	25.3	4500	3.2	2160	18.8
6039	900	25.3	4500	3.2	2205	18.8
6040	900	25.3	4500	3.2	2250	18.8
6041	900	25.3	4500	3.2	2295	18.8
6042	900	25.3	4500	3.2	2340	18.8
6043	900	25.3	4500	3.2	2385	18.8
6044	900	25.3	4500	3.2	2430	18.8
6045	900	25.3	4500	3.2	2475	18.8
6046	900	25.3	4500	3.2	2520	18.8
6047	900	25.3	4500	3.2	2565	18.8
6048	900	25.3	4500	3.2	2610	18.8
6049	900	25.3	4500	3.2	2655	18.8
6050	900	25.3	4500	3.2	2700	18.8
6051	900	25.3	4500	3.2	2745	18.8
6052	900	25.3	4500	3.2	2790	18.8
6053	900	25.3	4500	3.2	2835	18.8
6054	900	25.3	4500	3.2	2880	18.8
6055	900	25.3	4500	3.2	2925	18.8
6056	900	25.3	4500	3.2	2970	18.8
6130	850	24.4	4250	3.4	2982	17.6
6112	850	23.9	4250	3.4	2226	17.1
6113	850	23.9	4250	3.4	2268	17.1
6114	850	23.9	4250	3.4	2310	17.1
6115	850	23.9	4250	3.4	2352	17.1
6116	850	23.9	4250	3.4	2394	17.1
6117	850	23.9	4250	3.4	2436	17.1
6118	850	23.9	4250	3.4	2478	17.1
6119	850	23.9	4250	3.4	2520	17.1
6120	850	23.9	4250	3.4	2562	17.1
6121	850	23.9	4250	3.4	2604	17.1
6122	850	23.9	4250	3.4	2646	17.1
6123	850	23.9	4250	3.4	2688	17.1
6124	850	23.9	4250	3.4	2730	17.1
6125	850	23.9	4250	3.4	2772	17.1
6126	850	23.9	4250	3.4	2814	17.1
6127	850	23.9	4250	3.4	2856	17.1
6128	850	23.9	4250	3.4	2898	17.1
6129	850	23.9	4250	3.4	2940	17.1
6275	750	23.3	3750	3.3	2257	16.6
6190	800	23.0	4000	3.6	2240	15.7
6191	800	22.5	4000	3.6	2280	15.2
6192	800	22.5	4000	3.6	2320	15.2
6193	800	22.5	4000	3.6	2360	15.2
6194	800	22.5	4000	3.6	2400	15.2
6195	800	22.5	4000	3.6	2440	15.2
6196	800	22.5	4000	3.6	2480	15.2
6197	800	22.5	4000	3.6	2520	15.2
6198	800	22.5	4000	3.6	2560	15.2
6199	800	22.5	4000	3.6	2600	15.2
6200	800	22.5	4000	3.6	2640	15.2
6201	800	22.5	4000	3.6	2680	15.2
6202	800	22.5	4000	3.6	2720	15.2
6203	800	22.5	4000	3.6	2760	15.2
6204	800	22.5	4000	3.6	2800	15.2
6205	800	22.5	4000	3.6	2840	15.2
6206	800	22.5	4000	3.6	2880	15.2
6207	800	22.5	4000	3.6	2920	15.2
6208	800	22.5	4000	3.6	2960	15.2
6276	750	21.6	3750	3.9	2294	13.8
6367	700	21.6	3500	4.0	2310	13.6
6277	750	21.1	3750	3.9	2331	13.3
6278	750	21.1	3750	3.9	2368	13.3
6279	750	21.1	3750	3.9	2405	13.3
6280	750	21.1	3750	3.9	2442	13.3
6281	750	21.1	3750	3.9	2479	13.3
6282	750	21.1	3750	3.9	2516	13.3
6283	750	21.1	3750	3.9	2553	13.3
6284	750	21.1	3750	3.9	2590	13.3
6285	750	21.1	3750	3.9	2627	13.3
6286	750	21.1	3750	3.9	2664	13.3
6287	750	21.1	3750	3.9	2701	13.3
6288	750	21.1	3750	3.9	2738	13.3
6289	750	21.1	3750	3.9	2775	13.3
6290	750	21.1	3750	3.9	2812	13.3
6291	750	21.1	3750	3.9	2849	13.3
6292	750	21.1	3750	3.9	2886	13.3
6293	750	21.1	3750	3.9	2923	13.3
6294	750	21.1	3750	3.9	2960	13.3
6579	600	19.0	3000	3.0	2400	13.0
6368	700	20.1	3500	4.1	2345	11.8
6386	700	19.8	3500	4.1	2975	11.5
6469	650	20.3	3250	4.5	2368	11.4
6369	700	19.7	3500	4.1	2380	11.4
6370	700	19.7	3500	4.1	2415	11.4
6371	700	19.7	3500	4.1	2450	11.4
6372	700	19.7	3500	4.1	2485	11.4
6373	700	19.7	3500	4.1	2520	11.4
6374	700	19.7	3500	4.1	2555	11.4
6375	700	19.7	3500	4.1	2590	11.4
6376	700	19.7	3500	4.1	2625	11.4
6377	700	19.7	3500	4.1	2660	11.4
6378	700	19.7	3500	4.1	2695	11.4
6379	700	19.7	3500	4.1	2730	11.4
6380	700	19.7	3500	4.1	2765	11.4
6381	700	19.7	3500	4.1	2800	11.4
6382	700	19.7	3500	4.1	2835	11.4
6383	700	19.7	3500	4.1	2870	11.4
6384	700	19.7	3500	4.1	2905	11.4
6385	700	19.7	3500	4.1	2940	11.4
6470	650	18.6	3250	4.5	2400	9.6
6488	650	18.4	3250	4.5	2976	9.4
6471	650	18.3	3250	4.5	2432	9.3
6472	650	18.3	3250	4.5	2464	9.3
6473	650	18.3	3250	4.5	2496	9.3
6474	650	18.3	3250	4.5	2528	9.3
6475	650	18.3	3250	4.5	2560	9.3
6476	650	18.3	3250	4.5	2592	9.3
6477	650	18.3	3250	4.5	2624	9.3
6478	650	18.3	3250	4.5	2656	9.3
6479	650	18.3	3250	4.5	2688	9.3
6480	650	18.3	3250	4.5	2720	9.3
6481	650	18.3	3250	4.5	2752	9.3
6482	650	18.3	3250	4.5	2784	9.3
6483	650	18.3	3250	4.5	2816	9.3
6484	650	18.3	3250	4.5	2848	9.3
6485	650	18.3	3250	4.5	2880	9.3
6486	650	18.3	3250	4.5	2912	9.3
6487	650	18.3	3250	4.5	2944	9.3
6580	600	18.4	3000	4.8	2430	8.7
6839	500	15.8	2500	3.6	2500	8.6
6703	550	17.1	2750	4.5	2457	8.0
6581	600	16.9	3000	4.8	2460	7.2
6582	600	16.9	3000	4.8	2490	7.2
6583	600	16.9	3000	4.8	2520	7.2
6584	600	16.9	3000	4.8	2550	7.2
6585	600	16.9	3000	4.8	2580	7.2
6586	600	16.9	3000	4.8	2610	7.2
6587	600	16.9	3000	4.8	2640	7.2
6588	600	16.9	3000	4.8	2670	7.2
6589	600	16.9	3000	4.8	2700	7.2
6590	600	16.9	3000	4.8	2730	7.2
6591	600	16.9	3000	4.8	2760	7.2
6592	600	16.9	3000	4.8	2790	7.2
6593	600	16.9	3000	4.8	2820	7.2
6594	600	16.9	3000	4.8	2850	7.2
6595	600	16.9	3000	4.8	2880	7.2
6596	600	16.9	3000	4.8	2910	7.2
6597	600	16.9	3000	4.8	2940	7.2
6598	600	16.9	3000	4.8	2970	7.2

Overall score = score_ratio - 2 * repetitive DNA content