Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign14 - back to list

Summary
Image
Unique putative probes (251)
Putative probes (526)
Redundant probes (9)

Summary

`./analyse_probe_search_results : Tue Feb 20 17:41:08 2007`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign14
  assembly      : NCBIM36
  bias          : 3prime
  chromosome    : 16
  id            : 8
  strand        : 1
  -------------

Design window length: 3660bp

All jobs for probe design are successful - total: 59
Fetched conf : Exonerate mouse_NCBIM36 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 1179 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1179/1179
Putative probes that are unique                 : 251/1179
Putative probes exceeding selection criteria    : 786/1179 (both unique and not unique)
Putative probes with successfully-picked primers: 501/786

Identified 9 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
6882	1300	6500	-	260
6883	1300	6500	-	325
6919	1250	6250	-	248
6920	1250	6250	-	310
6921	1250	6250	-	372
6958	1200	6000	-	240
6959	1200	6000	-	300
6960	1200	6000	-	360
6961	1200	6000	-	420
7000	1150	5750	-	228
7001	1150	5750	-	285
7002	1150	5750	-	342
7003	1150	5750	-	399
7004	1150	5750	-	456
7045	1100	5500	-	220
7046	1100	5500	-	275
7047	1100	5500	-	330
7048	1100	5500	-	385
7049	1100	5500	-	440
7050	1100	5500	-	495
7093	1050	5250	-	260
7094	1050	5250	-	312
7095	1050	5250	-	364
7096	1050	5250	-	416
7097	1050	5250	-	468
7098	1050	5250	-	520
7099	1050	5250	-	572
7144	1000	5000	-	250
7145	1000	5000	-	300
7146	1000	5000	-	350
7147	1000	5000	-	400
7148	1000	5000	-	450
7149	1000	5000	-	500
7150	1000	5000	-	550
7151	1000	5000	-	600
7198	950	4750	-	235
7199	950	4750	-	282
7200	950	4750	-	329
7201	950	4750	-	376
7202	950	4750	-	423
7203	950	4750	-	470
7204	950	4750	-	517
7205	950	4750	-	564
7206	950	4750	-	611
7207	950	4750	-	658
7256	900	4500	-	225
7257	900	4500	-	270
7258	900	4500	-	315
7259	900	4500	-	360
7260	900	4500	-	405
7261	900	4500	-	450
7262	900	4500	-	495
7263	900	4500	-	540
7264	900	4500	-	585
7265	900	4500	-	630
7266	900	4500	-	675
7267	900	4500	-	720
7319	850	4250	-	252
7320	850	4250	-	294
7321	850	4250	-	336
7322	850	4250	-	378
7323	850	4250	-	420
7324	850	4250	-	462
7325	850	4250	-	504
7326	850	4250	-	546
7327	850	4250	-	588
7328	850	4250	-	630
7329	850	4250	-	672
7330	850	4250	-	714
7331	850	4250	-	756
7386	800	4000	-	240
7387	800	4000	-	280
7388	800	4000	-	320
7389	800	4000	-	360
7390	800	4000	-	400
7391	800	4000	-	440
7392	800	4000	-	480
7393	800	4000	-	520
7394	800	4000	-	560
7395	800	4000	-	600
7396	800	4000	-	640
7397	800	4000	-	680
7398	800	4000	-	720
7399	800	4000	-	760
7400	800	4000	-	800
7458	750	3750	-	222
7459	750	3750	-	259
7460	750	3750	-	296
7461	750	3750	-	333
7462	750	3750	-	370
7463	750	3750	-	407
7464	750	3750	-	444
7465	750	3750	-	481
7466	750	3750	-	518
7467	750	3750	-	555
7468	750	3750	-	592
7469	750	3750	-	629
7470	750	3750	-	666
7471	750	3750	-	703
7472	750	3750	-	740
7473	750	3750	-	777
7474	750	3750	-	814
7475	750	3750	-	851
7476	750	3750	-	888
7538	700	3500	-	245
7539	700	3500	-	280
7540	700	3500	-	315
7541	700	3500	-	350
7542	700	3500	-	385
7543	700	3500	-	420
7544	700	3500	-	455
7545	700	3500	-	490
7546	700	3500	-	525
7547	700	3500	-	560
7548	700	3500	-	595
7549	700	3500	-	630
7550	700	3500	-	665
7551	700	3500	-	700
7552	700	3500	-	735
7553	700	3500	-	770
7554	700	3500	-	805
7555	700	3500	-	840
7556	700	3500	-	875
7557	700	3500	-	910
7623	650	3250	-	224
7624	650	3250	-	256
7625	650	3250	-	288
7626	650	3250	-	320
7627	650	3250	-	352
7628	650	3250	-	384
7629	650	3250	-	416
7630	650	3250	-	448
7631	650	3250	-	480
7632	650	3250	-	512
7633	650	3250	-	544
7634	650	3250	-	576
7635	650	3250	-	608
7636	650	3250	-	640
7637	650	3250	-	672
7638	650	3250	-	704
7639	650	3250	-	736
7640	650	3250	-	768
7641	650	3250	-	800
7642	650	3250	-	832
7643	650	3250	-	864
7644	650	3250	-	896
7645	650	3250	-	928
7646	650	3250	-	960
7719	600	3000	-	240
7720	600	3000	-	270
7721	600	3000	-	300
7722	600	3000	-	330
7723	600	3000	-	360
7724	600	3000	-	390
7725	600	3000	-	420
7726	600	3000	-	450
7727	600	3000	-	480
7728	600	3000	-	510
7729	600	3000	-	540
7730	600	3000	-	570
7731	600	3000	-	600
7732	600	3000	-	630
7733	600	3000	-	660
7734	600	3000	-	690
7735	600	3000	-	720
7736	600	3000	-	750
7737	600	3000	-	780
7738	600	3000	-	810
7739	600	3000	-	840
7740	600	3000	-	870
7741	600	3000	-	900
7742	600	3000	-	930
7743	600	3000	-	960
7744	600	3000	-	990
7745	600	3000	-	1020
7822	550	2750	-	216
7823	550	2750	-	243
7824	550	2750	-	270
7825	550	2750	-	297
7826	550	2750	-	324
7827	550	2750	-	351
7828	550	2750	-	378
7829	550	2750	-	405
7830	550	2750	-	432
7831	550	2750	-	459
7832	550	2750	-	486
7833	550	2750	-	513
7834	550	2750	-	540
7835	550	2750	-	567
7836	550	2750	-	594
7837	550	2750	-	621
7838	550	2750	-	648
7839	550	2750	-	675
7840	550	2750	-	702
7841	550	2750	-	729
7842	550	2750	-	756
7843	550	2750	-	783
7844	550	2750	-	810
7845	550	2750	-	837
7846	550	2750	-	864
7847	550	2750	-	891
7848	550	2750	-	918
7849	550	2750	-	945
7850	550	2750	-	972
7851	550	2750	-	999
7852	550	2750	-	1026
7853	550	2750	-	1053
7854	550	2750	-	1080
7915	550	2750	-	2727
7916	550	2750	-	2754
7939	500	2500	-	225
7940	500	2500	-	250
7941	500	2500	-	275
7942	500	2500	-	300
7943	500	2500	-	325
7944	500	2500	-	350
7945	500	2500	-	375
7946	500	2500	-	400
7947	500	2500	-	425
7948	500	2500	-	450
7949	500	2500	-	475
7950	500	2500	-	500
7951	500	2500	-	525
7952	500	2500	-	550
7953	500	2500	-	575
7954	500	2500	-	600
7955	500	2500	-	625
7956	500	2500	-	650
7957	500	2500	-	675
7958	500	2500	-	700
7959	500	2500	-	725
7960	500	2500	-	750
7961	500	2500	-	775
7962	500	2500	-	800
7963	500	2500	-	825
7964	500	2500	-	850
7965	500	2500	-	875
7966	500	2500	-	900
7967	500	2500	-	925
7968	500	2500	-	950
7969	500	2500	-	975
7970	500	2500	-	1000
7971	500	2500	-	1025
7972	500	2500	-	1050
7973	500	2500	-	1075
7974	500	2500	-	1100
7975	500	2500	-	1125
8039	500	2500	-	2725
8040	500	2500	-	2750
8041	500	2500	-	2775
8042	500	2500	-	2800

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
6878	1300	41.4	6500	-	0	41.4
6879	1300	41.4	6500	-	65	41.4
6880	1300	41.4	6500	-	130	41.4
6881	1300	41.4	6500	-	195	41.4
6884	1300	40.1	6500	-	390	40.1
6885	1300	40.1	6500	-	455	40.1
6886	1300	40.1	6500	-	520	40.1
6887	1300	40.1	6500	-	585	40.1
6888	1300	40.1	6500	-	650	40.1
6889	1300	40.1	6500	-	715	40.1
6890	1300	40.1	6500	-	780	40.1
6891	1300	40.1	6500	-	845	40.1
6892	1300	40.1	6500	-	910	40.1
6915	1250	39.8	6250	-	0	39.8
6916	1250	39.8	6250	-	62	39.8
6917	1250	39.8	6250	-	124	39.8
6918	1250	39.8	6250	-	186	39.8
6995	1200	38.7	6000	-	2460	38.7
6922	1250	38.6	6250	-	434	38.6
6923	1250	38.6	6250	-	496	38.6
6924	1250	38.6	6250	-	558	38.6
6925	1250	38.6	6250	-	620	38.6
6926	1250	38.6	6250	-	682	38.6
6927	1250	38.6	6250	-	744	38.6
6928	1250	38.6	6250	-	806	38.6
6929	1250	38.6	6250	-	868	38.6
6930	1250	38.6	6250	-	930	38.6
6954	1200	38.2	6000	-	0	38.2
6955	1200	38.2	6000	-	60	38.2
6956	1200	38.2	6000	-	120	38.2
6957	1200	38.2	6000	-	180	38.2
7005	1150	37.3	5750	-	513	37.3
7039	1150	37.1	5750	-	2451	37.1
7040	1150	37.1	5750	-	2508	37.1
6962	1200	37.0	6000	-	480	37.0
6963	1200	37.0	6000	-	540	37.0
6964	1200	37.0	6000	-	600	37.0
6965	1200	37.0	6000	-	660	37.0
6966	1200	37.0	6000	-	720	37.0
6967	1200	37.0	6000	-	780	37.0
6968	1200	37.0	6000	-	840	37.0
6969	1200	37.0	6000	-	900	37.0
6970	1200	37.0	6000	-	960	37.0
6996	1150	36.6	5750	-	0	36.6
6997	1150	36.6	5750	-	57	36.6
6998	1150	36.6	5750	-	114	36.6
6999	1150	36.6	5750	-	171	36.6
6971	1200	37.0	6000	0.2	1020	36.5
7051	1100	35.7	5500	-	550	35.7
7006	1150	35.5	5750	-	570	35.5
7007	1150	35.5	5750	-	627	35.5
7008	1150	35.5	5750	-	684	35.5
7009	1150	35.5	5750	-	741	35.5
7010	1150	35.5	5750	-	798	35.5
7011	1150	35.5	5750	-	855	35.5
7012	1150	35.5	5750	-	912	35.5
7013	1150	35.5	5750	-	969	35.5
7014	1150	35.5	5750	-	1026	35.5
7085	1100	35.5	5500	-	2420	35.5
7086	1100	35.5	5500	-	2475	35.5
7087	1100	35.5	5500	-	2530	35.5
6994	1200	38.7	6000	1.7	2400	35.4
7041	1100	35.0	5500	-	0	35.0
7042	1100	35.0	5500	-	55	35.0
7043	1100	35.0	5500	-	110	35.0
7044	1100	35.0	5500	-	165	35.0
7092	1050	34.3	5250	-	208	34.3
7052	1100	34.0	5500	-	605	34.0
7053	1100	34.0	5500	-	660	34.0
7054	1100	34.0	5500	-	715	34.0
7055	1100	34.0	5500	-	770	34.0
7056	1100	34.0	5500	-	825	34.0
7057	1100	34.0	5500	-	880	34.0
7058	1100	34.0	5500	-	935	34.0
7059	1100	34.0	5500	-	990	34.0
7060	1100	34.0	5500	-	1045	34.0
7061	1100	34.0	5500	-	1100	34.0
7135	1050	33.9	5250	-	2444	33.9
7136	1050	33.9	5250	-	2496	33.9
7137	1050	33.9	5250	-	2548	33.9
7138	1050	33.9	5250	-	2600	33.9
7088	1050	33.4	5250	-	0	33.4
7089	1050	33.4	5250	-	52	33.4
7090	1050	33.4	5250	-	104	33.4
7091	1050	33.4	5250	-	156	33.4
7015	1150	35.5	5750	1.4	1083	32.7
7038	1150	37.1	5750	2.3	2394	32.6
7152	1000	32.5	5000	-	650	32.5
7100	1050	32.4	5250	-	624	32.4
7101	1050	32.4	5250	-	676	32.4
7102	1050	32.4	5250	-	728	32.4
7103	1050	32.4	5250	-	780	32.4
7104	1050	32.4	5250	-	832	32.4
7105	1050	32.4	5250	-	884	32.4
7106	1050	32.4	5250	-	936	32.4
7107	1050	32.4	5250	-	988	32.4
7108	1050	32.4	5250	-	1040	32.4
7109	1050	32.4	5250	-	1092	32.4
7110	1050	32.4	5250	-	1144	32.4
7188	1000	32.3	5000	-	2450	32.3
7189	1000	32.3	5000	-	2500	32.3
7190	1000	32.3	5000	-	2550	32.3
7191	1000	32.3	5000	-	2600	32.3
7192	1000	32.3	5000	-	2650	32.3
7139	1000	31.8	5000	-	0	31.8
7140	1000	31.8	5000	-	50	31.8
7141	1000	31.8	5000	-	100	31.8
7142	1000	31.8	5000	-	150	31.8
7143	1000	31.8	5000	-	200	31.8
7153	1000	30.9	5000	-	700	30.9
7154	1000	30.9	5000	-	750	30.9
7155	1000	30.9	5000	-	800	30.9
7156	1000	30.9	5000	-	850	30.9
7157	1000	30.9	5000	-	900	30.9
7158	1000	30.9	5000	-	950	30.9
7159	1000	30.9	5000	-	1000	30.9
7160	1000	30.9	5000	-	1050	30.9
7161	1000	30.9	5000	-	1100	30.9
7162	1000	30.9	5000	-	1150	30.9
7163	1000	30.9	5000	-	1200	30.9
7208	950	30.8	4750	-	705	30.8
7245	950	30.6	4750	-	2444	30.6
7246	950	30.6	4750	-	2491	30.6
7247	950	30.6	4750	-	2538	30.6
7248	950	30.6	4750	-	2585	30.6
7249	950	30.6	4750	-	2632	30.6
7250	950	30.6	4750	-	2679	30.6
6931	1250	34.5	6250	2.0	992	30.5
7193	950	30.3	4750	-	0	30.3
7194	950	30.3	4750	-	47	30.3
7195	950	30.3	4750	-	94	30.3
7196	950	30.3	4750	-	141	30.3
7197	950	30.3	4750	-	188	30.3
7312	900	29.8	4500	-	2745	29.8
7209	950	29.3	4750	-	752	29.3
7210	950	29.3	4750	-	799	29.3
7211	950	29.3	4750	-	846	29.3
7212	950	29.3	4750	-	893	29.3
7213	950	29.3	4750	-	940	29.3
7214	950	29.3	4750	-	987	29.3
7215	950	29.3	4750	-	1034	29.3
7216	950	29.3	4750	-	1081	29.3
7217	950	29.3	4750	-	1128	29.3
7218	950	29.3	4750	-	1175	29.3
7219	950	29.3	4750	-	1222	29.3
7268	900	29.2	4500	-	765	29.2
7305	900	29.0	4500	-	2430	29.0
7306	900	29.0	4500	-	2475	29.0
7307	900	29.0	4500	-	2520	29.0
7308	900	29.0	4500	-	2565	29.0
7309	900	29.0	4500	-	2610	29.0
7310	900	29.0	4500	-	2655	29.0
7311	900	29.0	4500	-	2700	29.0
7220	950	29.3	4750	0.2	1269	28.9
7251	900	28.7	4500	-	0	28.7
7252	900	28.7	4500	-	45	28.7
7253	900	28.7	4500	-	90	28.7
7254	900	28.7	4500	-	135	28.7
7255	900	28.7	4500	-	180	28.7
7134	1050	33.9	5250	2.7	2392	28.5
7378	850	28.1	4250	-	2730	28.1
7379	850	28.1	4250	-	2772	28.1
7318	850	27.8	4250	-	210	27.8
7269	900	27.8	4500	-	810	27.8
7270	900	27.8	4500	-	855	27.8
7271	900	27.8	4500	-	900	27.8
7272	900	27.8	4500	-	945	27.8
7273	900	27.8	4500	-	990	27.8
7274	900	27.8	4500	-	1035	27.8
7275	900	27.8	4500	-	1080	27.8
7276	900	27.8	4500	-	1125	27.8
7277	900	27.8	4500	-	1170	27.8
7278	900	27.8	4500	-	1215	27.8
7279	900	27.8	4500	-	1260	27.8
7280	900	27.8	4500	-	1305	27.8
7332	850	27.6	4250	-	798	27.6
7371	850	27.4	4250	-	2436	27.4
7372	850	27.4	4250	-	2478	27.4
7373	850	27.4	4250	-	2520	27.4
7374	850	27.4	4250	-	2562	27.4
7375	850	27.4	4250	-	2604	27.4
7376	850	27.4	4250	-	2646	27.4
7377	850	27.4	4250	-	2688	27.4
7313	850	27.1	4250	-	0	27.1
7314	850	27.1	4250	-	42	27.1
7315	850	27.1	4250	-	84	27.1
7316	850	27.1	4250	-	126	27.1
7317	850	27.1	4250	-	168	27.1
7448	800	26.5	4000	-	2720	26.5
7449	800	26.5	4000	-	2760	26.5
7450	800	26.5	4000	-	2800	26.5
7451	800	26.5	4000	-	2840	26.5
7333	850	26.2	4250	-	840	26.2
7334	850	26.2	4250	-	882	26.2
7335	850	26.2	4250	-	924	26.2
7336	850	26.2	4250	-	966	26.2
7337	850	26.2	4250	-	1008	26.2
7338	850	26.2	4250	-	1050	26.2
7339	850	26.2	4250	-	1092	26.2
7340	850	26.2	4250	-	1134	26.2
7341	850	26.2	4250	-	1176	26.2
7342	850	26.2	4250	-	1218	26.2
7343	850	26.2	4250	-	1260	26.2
7344	850	26.2	4250	-	1302	26.2
7345	850	26.2	4250	-	1344	26.2
7401	800	26.0	4000	-	840	26.0
7441	800	25.8	4000	-	2440	25.8
7442	800	25.8	4000	-	2480	25.8
7443	800	25.8	4000	-	2520	25.8
7444	800	25.8	4000	-	2560	25.8
7445	800	25.8	4000	-	2600	25.8
7446	800	25.8	4000	-	2640	25.8
7447	800	25.8	4000	-	2680	25.8
7380	800	25.5	4000	-	0	25.5
7381	800	25.5	4000	-	40	25.5
7382	800	25.5	4000	-	80	25.5
7383	800	25.5	4000	-	120	25.5
7384	800	25.5	4000	-	160	25.5
7385	800	25.5	4000	-	200	25.5
7526	750	24.8	3750	-	2738	24.8
7527	750	24.8	3750	-	2775	24.8
7528	750	24.8	3750	-	2812	24.8
7529	750	24.8	3750	-	2849	24.8
7530	750	24.8	3750	-	2886	24.8
7402	800	24.7	4000	-	880	24.7
7403	800	24.7	4000	-	920	24.7
7404	800	24.7	4000	-	960	24.7
7405	800	24.7	4000	-	1000	24.7
7406	800	24.7	4000	-	1040	24.7
7407	800	24.7	4000	-	1080	24.7
7408	800	24.7	4000	-	1120	24.7
7409	800	24.7	4000	-	1160	24.7
7410	800	24.7	4000	-	1200	24.7
7411	800	24.7	4000	-	1240	24.7
7412	800	24.7	4000	-	1280	24.7
7413	800	24.7	4000	-	1320	24.7
7414	800	24.7	4000	-	1360	24.7
7415	800	24.7	4000	-	1400	24.7
7518	750	24.2	3750	-	2442	24.2
7519	750	24.2	3750	-	2479	24.2
7520	750	24.2	3750	-	2516	24.2
7521	750	24.2	3750	-	2553	24.2
7522	750	24.2	3750	-	2590	24.2
7523	750	24.2	3750	-	2627	24.2
7524	750	24.2	3750	-	2664	24.2
7525	750	24.2	3750	-	2701	24.2
7452	750	23.9	3750	-	0	23.9
7453	750	23.9	3750	-	37	23.9
7454	750	23.9	3750	-	74	23.9
7455	750	23.9	3750	-	111	23.9
7456	750	23.9	3750	-	148	23.9
7457	750	23.9	3750	-	185	23.9
7111	1050	29.0	5250	2.8	1196	23.5
7609	700	23.2	3500	-	2730	23.2
7610	700	23.2	3500	-	2765	23.2
7611	700	23.2	3500	-	2800	23.2
7612	700	23.2	3500	-	2835	23.2
7613	700	23.2	3500	-	2870	23.2
7614	700	23.2	3500	-	2905	23.2
7615	700	23.2	3500	-	2940	23.2
7477	750	23.1	3750	-	925	23.1
7478	750	23.1	3750	-	962	23.1
7479	750	23.1	3750	-	999	23.1
7480	750	23.1	3750	-	1036	23.1
7481	750	23.1	3750	-	1073	23.1
7482	750	23.1	3750	-	1110	23.1
7483	750	23.1	3750	-	1147	23.1
7484	750	23.1	3750	-	1184	23.1
7485	750	23.1	3750	-	1221	23.1
7486	750	23.1	3750	-	1258	23.1
7487	750	23.1	3750	-	1295	23.1
7488	750	23.1	3750	-	1332	23.1
7489	750	23.1	3750	-	1369	23.1
7490	750	23.1	3750	-	1406	23.1
7491	750	23.1	3750	-	1443	23.1
7537	700	22.9	3500	-	210	22.9
7558	700	22.7	3500	-	945	22.7
7601	700	22.6	3500	-	2450	22.6
7602	700	22.6	3500	-	2485	22.6
7603	700	22.6	3500	-	2520	22.6
7604	700	22.6	3500	-	2555	22.6
7605	700	22.6	3500	-	2590	22.6
7606	700	22.6	3500	-	2625	22.6
7607	700	22.6	3500	-	2660	22.6
7608	700	22.6	3500	-	2695	22.6
7531	700	22.3	3500	-	0	22.3
7532	700	22.3	3500	-	35	22.3
7533	700	22.3	3500	-	70	22.3
7534	700	22.3	3500	-	105	22.3
7535	700	22.3	3500	-	140	22.3
7536	700	22.3	3500	-	175	22.3
7346	850	26.2	4250	2.2	1386	21.8
7559	700	21.6	3500	-	980	21.6
7560	700	21.6	3500	-	1015	21.6
7561	700	21.6	3500	-	1050	21.6
7562	700	21.6	3500	-	1085	21.6
7563	700	21.6	3500	-	1120	21.6
7564	700	21.6	3500	-	1155	21.6
7565	700	21.6	3500	-	1190	21.6
7566	700	21.6	3500	-	1225	21.6
7567	700	21.6	3500	-	1260	21.6
7568	700	21.6	3500	-	1295	21.6
7569	700	21.6	3500	-	1330	21.6
7570	700	21.6	3500	-	1365	21.6
7571	700	21.6	3500	-	1400	21.6
7572	700	21.6	3500	-	1435	21.6
7573	700	21.6	3500	-	1470	21.6
7574	700	21.6	3500	-	1505	21.6
7701	650	21.5	3250	-	2720	21.5
7702	650	21.5	3250	-	2752	21.5
7703	650	21.5	3250	-	2784	21.5
7704	650	21.5	3250	-	2816	21.5
7705	650	21.5	3250	-	2848	21.5
7706	650	21.5	3250	-	2880	21.5
7707	650	21.5	3250	-	2912	21.5
7708	650	21.5	3250	-	2944	21.5
7709	650	21.5	3250	-	2976	21.5
7710	650	21.5	3250	-	3008	21.5
7062	1100	28.4	5500	3.5	1155	21.4
7600	700	22.6	3500	0.7	2415	21.2
7647	650	21.1	3250	-	992	21.1
7164	1000	27.6	5000	3.3	1250	21.0
7692	650	21.0	3250	-	2432	21.0
7693	650	21.0	3250	-	2464	21.0
7694	650	21.0	3250	-	2496	21.0
7695	650	21.0	3250	-	2528	21.0
7696	650	21.0	3250	-	2560	21.0
7697	650	21.0	3250	-	2592	21.0
7698	650	21.0	3250	-	2624	21.0
7699	650	21.0	3250	-	2656	21.0
7700	650	21.0	3250	-	2688	21.0
7616	650	20.7	3250	-	0	20.7
7617	650	20.7	3250	-	32	20.7
7618	650	20.7	3250	-	64	20.7
7619	650	20.7	3250	-	96	20.7
7620	650	20.7	3250	-	128	20.7
7621	650	20.7	3250	-	160	20.7
7622	650	20.7	3250	-	192	20.7
7648	650	20.1	3250	-	1024	20.1
7649	650	20.1	3250	-	1056	20.1
7650	650	20.1	3250	-	1088	20.1
7651	650	20.1	3250	-	1120	20.1
7652	650	20.1	3250	-	1152	20.1
7653	650	20.1	3250	-	1184	20.1
7654	650	20.1	3250	-	1216	20.1
7655	650	20.1	3250	-	1248	20.1
7656	650	20.1	3250	-	1280	20.1
7657	650	20.1	3250	-	1312	20.1
7658	650	20.1	3250	-	1344	20.1
7659	650	20.1	3250	-	1376	20.1
7660	650	20.1	3250	-	1408	20.1
7661	650	20.1	3250	-	1440	20.1
7662	650	20.1	3250	-	1472	20.1
7663	650	20.1	3250	-	1504	20.1
7664	650	20.1	3250	-	1536	20.1
7802	600	19.9	3000	-	2730	19.9
7803	600	19.9	3000	-	2760	19.9
7804	600	19.9	3000	-	2790	19.9
7805	600	19.9	3000	-	2820	19.9
7806	600	19.9	3000	-	2850	19.9
7807	600	19.9	3000	-	2880	19.9
7808	600	19.9	3000	-	2910	19.9
7809	600	19.9	3000	-	2940	19.9
7810	600	19.9	3000	-	2970	19.9
7811	600	19.9	3000	-	3000	19.9
7812	600	19.9	3000	-	3030	19.9
7813	600	19.9	3000	-	3060	19.9
7304	900	27.6	4500	3.9	2385	19.8
7665	650	20.1	3250	0.2	1568	19.8
7492	750	23.1	3750	1.7	1480	19.7
7718	600	19.6	3000	-	210	19.6
7746	600	19.5	3000	-	1050	19.5
7792	600	19.4	3000	-	2430	19.4
7793	600	19.4	3000	-	2460	19.4
7794	600	19.4	3000	-	2490	19.4
7795	600	19.4	3000	-	2520	19.4
7796	600	19.4	3000	-	2550	19.4
7797	600	19.4	3000	-	2580	19.4
7798	600	19.4	3000	-	2610	19.4
7799	600	19.4	3000	-	2640	19.4
7800	600	19.4	3000	-	2670	19.4
7801	600	19.4	3000	-	2700	19.4
7711	600	19.1	3000	-	0	19.1
7712	600	19.1	3000	-	30	19.1
7713	600	19.1	3000	-	60	19.1
7714	600	19.1	3000	-	90	19.1
7715	600	19.1	3000	-	120	19.1
7716	600	19.1	3000	-	150	19.1
7717	600	19.1	3000	-	180	19.1
7747	600	18.5	3000	-	1080	18.5
7748	600	18.5	3000	-	1110	18.5
7749	600	18.5	3000	-	1140	18.5
7750	600	18.5	3000	-	1170	18.5
7751	600	18.5	3000	-	1200	18.5
7752	600	18.5	3000	-	1230	18.5
7753	600	18.5	3000	-	1260	18.5
7754	600	18.5	3000	-	1290	18.5
7755	600	18.5	3000	-	1320	18.5
7756	600	18.5	3000	-	1350	18.5
7757	600	18.5	3000	-	1380	18.5
7758	600	18.5	3000	-	1410	18.5
7759	600	18.5	3000	-	1440	18.5
7760	600	18.5	3000	-	1470	18.5
7761	600	18.5	3000	-	1500	18.5
7762	600	18.5	3000	-	1530	18.5
7763	600	18.5	3000	-	1560	18.5
7764	600	18.5	3000	-	1590	18.5
7765	600	19.5	3000	0.5	1620	18.5
7917	550	18.2	2750	-	2781	18.2
7918	550	18.2	2750	-	2808	18.2
7919	550	18.2	2750	-	2835	18.2
7920	550	18.2	2750	-	2862	18.2
7921	550	18.2	2750	-	2889	18.2
7922	550	18.2	2750	-	2916	18.2
7923	550	18.2	2750	-	2943	18.2
7924	550	18.2	2750	-	2970	18.2
7925	550	18.2	2750	-	2997	18.2
7926	550	18.2	2750	-	3024	18.2
7927	550	18.2	2750	-	3051	18.2
7928	550	18.2	2750	-	3078	18.2
7929	550	18.2	2750	-	3105	18.2
7855	550	17.9	2750	-	1107	17.9
7874	550	17.9	2750	-	1620	17.9
7875	550	17.9	2750	-	1647	17.9
7904	550	17.7	2750	-	2430	17.7
7905	550	17.7	2750	-	2457	17.7
7906	550	17.7	2750	-	2484	17.7
7907	550	17.7	2750	-	2511	17.7
7908	550	17.7	2750	-	2538	17.7
7909	550	17.7	2750	-	2565	17.7
7910	550	17.7	2750	-	2592	17.7
7911	550	17.7	2750	-	2619	17.7
7912	550	17.7	2750	-	2646	17.7
7913	550	17.7	2750	-	2673	17.7
7914	550	17.7	2750	-	2700	17.7
7281	900	24.9	4500	3.7	1350	17.5
7814	550	17.5	2750	-	0	17.5
7815	550	17.5	2750	-	27	17.5
7816	550	17.5	2750	-	54	17.5
7817	550	17.5	2750	-	81	17.5
7818	550	17.5	2750	-	108	17.5
7819	550	17.5	2750	-	135	17.5
7820	550	17.5	2750	-	162	17.5
7821	550	17.5	2750	-	189	17.5
7856	550	17.0	2750	-	1134	17.0
7857	550	17.0	2750	-	1161	17.0
7858	550	17.0	2750	-	1188	17.0
7859	550	17.0	2750	-	1215	17.0
7860	550	17.0	2750	-	1242	17.0
7861	550	17.0	2750	-	1269	17.0
7862	550	17.0	2750	-	1296	17.0
7863	550	17.0	2750	-	1323	17.0
7864	550	17.0	2750	-	1350	17.0
7865	550	17.0	2750	-	1377	17.0
7866	550	17.0	2750	-	1404	17.0
7867	550	17.0	2750	-	1431	17.0
7868	550	17.0	2750	-	1458	17.0
7869	550	17.0	2750	-	1485	17.0
7870	550	17.0	2750	-	1512	17.0
7871	550	17.0	2750	-	1539	17.0
7872	550	17.0	2750	-	1566	17.0
7873	550	17.0	2750	-	1593	17.0
7084	1100	26.8	5500	5.0	2365	16.8
8043	500	16.7	2500	-	2825	16.7
7416	800	22.3	4000	2.9	1440	16.6
8044	500	16.6	2500	-	2850	16.6
8045	500	16.6	2500	-	2875	16.6
8046	500	16.6	2500	-	2900	16.6
8047	500	16.6	2500	-	2925	16.6
8048	500	16.6	2500	-	2950	16.6
8049	500	16.6	2500	-	2975	16.6
8050	500	16.6	2500	-	3000	16.6
8051	500	16.6	2500	-	3025	16.6
8052	500	16.6	2500	-	3050	16.6
8053	500	16.6	2500	-	3075	16.6
8054	500	16.6	2500	-	3100	16.6
8055	500	16.6	2500	-	3125	16.6
8056	500	16.6	2500	-	3150	16.6
7976	500	16.2	2500	-	1150	16.2
7994	500	16.2	2500	-	1600	16.2
7995	500	16.2	2500	-	1625	16.2
7996	500	16.2	2500	-	1650	16.2
7997	500	16.2	2500	-	1675	16.2
7998	500	16.2	2500	-	1700	16.2
8027	500	16.1	2500	-	2425	16.1
8028	500	16.1	2500	-	2450	16.1
8029	500	16.1	2500	-	2475	16.1
8030	500	16.1	2500	-	2500	16.1
8031	500	16.1	2500	-	2525	16.1
8032	500	16.1	2500	-	2550	16.1
8033	500	16.1	2500	-	2575	16.1
8034	500	16.1	2500	-	2600	16.1
8035	500	16.1	2500	-	2625	16.1
8036	500	16.1	2500	-	2650	16.1
8037	500	16.1	2500	-	2675	16.1
8038	500	16.1	2500	-	2700	16.1
7930	500	15.9	2500	-	0	15.9
7931	500	15.9	2500	-	25	15.9
7932	500	15.9	2500	-	50	15.9
7933	500	15.9	2500	-	75	15.9
7934	500	15.9	2500	-	100	15.9
7935	500	15.9	2500	-	125	15.9
7936	500	15.9	2500	-	150	15.9
7937	500	15.9	2500	-	175	15.9
7938	500	15.9	2500	-	200	15.9
7977	500	15.4	2500	-	1175	15.4
7978	500	15.4	2500	-	1200	15.4
7979	500	15.4	2500	-	1225	15.4
7980	500	15.4	2500	-	1250	15.4
7981	500	15.4	2500	-	1275	15.4
7982	500	15.4	2500	-	1300	15.4
7983	500	15.4	2500	-	1325	15.4
7984	500	15.4	2500	-	1350	15.4
7985	500	15.4	2500	-	1375	15.4
7986	500	15.4	2500	-	1400	15.4
7987	500	15.4	2500	-	1425	15.4
7988	500	15.4	2500	-	1450	15.4
7989	500	15.4	2500	-	1475	15.4
7990	500	15.4	2500	-	1500	15.4
7991	500	15.4	2500	-	1525	15.4
7992	500	15.4	2500	-	1550	15.4
7993	500	15.4	2500	-	1575	15.4
7876	550	17.9	2750	1.3	1674	15.3
6893	1300	22.8	6500	4.5	975	13.9
7999	500	16.2	2500	1.6	1725	13.0
7575	700	19.6	3500	3.3	1540	13.0

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
7187	1000	32.3	5000	2.0	2400	28.3
7244	950	30.6	4750	2.4	2397	25.8
7370	850	27.4	4250	3.1	2394	21.3
7440	800	25.8	4000	2.5	2400	20.8
7517	750	24.2	3750	2.0	2405	20.2
7691	650	21.0	3250	3.1	2400	14.8
7791	600	19.4	3000	3.3	2400	12.7
7903	550	17.7	2750	3.1	2403	11.6
8026	500	16.1	2500	4.0	2400	8.1

Overall score = score_ratio - 2 * repetitive DNA content