Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign16 - back to list

Summary
Image
Unique putative probes (156)
Putative probes (316)
Redundant probes (248)

Summary

`./analyse_probe_search_results : Tue Feb 13 12:54:38 2007`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign16
  assembly      : NCBIM36
  bias          : 5prime
  chromosome    : 16
  id            : 3
  strand        : 1
  -------------

Design window length: 4260bp

All jobs for probe design are successful - total: 72
Fetched conf : Exonerate mouse_NCBIM36 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 1423 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1423/1423
Putative probes that are unique                 : 156/1423
Putative probes exceeding selection criteria    : 720/1423 (both unique and not unique)
Putative probes with successfully-picked primers: 500/720

Identified 248 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
2707	900	4500	-	3330
2749	850	4250	-	1722
2750	850	4250	-	1764
2787	850	4250	-	3318
2788	850	4250	-	3360
2789	850	4250	-	3402
2833	800	4000	-	1720
2834	800	4000	-	1760
2835	800	4000	-	1800
2873	800	4000	-	3320
2874	800	4000	-	3360
2875	800	4000	-	3400
2876	800	4000	-	3440
2924	750	3750	-	1739
2925	750	3750	-	1776
2926	750	3750	-	1813
2927	750	3750	-	1850
2967	750	3750	-	3330
2968	750	3750	-	3367
2969	750	3750	-	3404
2970	750	3750	-	3441
2971	750	3750	-	3478
3021	700	3500	-	1715
3022	700	3500	-	1750
3023	700	3500	-	1785
3024	700	3500	-	1820
3025	700	3500	-	1855
3026	700	3500	-	1890
3027	700	3500	-	1925
3067	700	3500	-	3325
3068	700	3500	-	3360
3069	700	3500	-	3395
3070	700	3500	-	3430
3071	700	3500	-	3465
3072	700	3500	-	3500
3073	700	3500	-	3535
3128	650	3250	-	1728
3129	650	3250	-	1760
3130	650	3250	-	1792
3131	650	3250	-	1824
3132	650	3250	-	1856
3133	650	3250	-	1888
3134	650	3250	-	1920
3135	650	3250	-	1952
3178	650	3250	-	3328
3179	650	3250	-	3360
3180	650	3250	-	3392
3181	650	3250	-	3424
3182	650	3250	-	3456
3183	650	3250	-	3488
3184	650	3250	-	3520
3185	650	3250	-	3552
3186	650	3250	-	3584
3245	600	3000	-	1740
3246	600	3000	-	1770
3247	600	3000	-	1800
3248	600	3000	-	1830
3249	600	3000	-	1860
3250	600	3000	-	1890
3251	600	3000	-	1920
3252	600	3000	-	1950
3253	600	3000	-	1980
3254	600	3000	-	2010
3298	600	3000	-	3330
3299	600	3000	-	3360
3300	600	3000	-	3390
3301	600	3000	-	3420
3302	600	3000	-	3450
3303	600	3000	-	3480
3304	600	3000	-	3510
3305	600	3000	-	3540
3306	600	3000	-	3570
3307	600	3000	-	3600
3308	600	3000	-	3630
3309	550	2750	-	0
3373	550	2750	-	1728
3374	550	2750	-	1755
3375	550	2750	-	1782
3376	550	2750	-	1809
3377	550	2750	-	1836
3378	550	2750	-	1863
3379	550	2750	-	1890
3380	550	2750	-	1917
3381	550	2750	-	1944
3382	550	2750	-	1971
3383	550	2750	-	1998
3384	550	2750	-	2025
3385	550	2750	-	2052
3432	550	2750	-	3321
3433	550	2750	-	3348
3434	550	2750	-	3375
3435	550	2750	-	3402
3436	550	2750	-	3429
3437	550	2750	-	3456
3438	550	2750	-	3483
3439	550	2750	-	3510
3440	550	2750	-	3537
3441	550	2750	-	3564
3442	550	2750	-	3591
3443	550	2750	-	3618
3444	550	2750	-	3645
3445	550	2750	-	3672
3446	550	2750	-	3699
3447	500	2500	-	0
3448	500	2500	-	25
3449	500	2500	-	50
3493	500	2500	-	1150
3494	500	2500	-	1175
3516	500	2500	-	1725
3517	500	2500	-	1750
3518	500	2500	-	1775
3519	500	2500	-	1800
3520	500	2500	-	1825
3521	500	2500	-	1850
3522	500	2500	-	1875
3523	500	2500	-	1900
3524	500	2500	-	1925
3525	500	2500	-	1950
3526	500	2500	-	1975
3527	500	2500	-	2000
3528	500	2500	-	2025
3529	500	2500	-	2050
3530	500	2500	-	2075
3531	500	2500	-	2100
3532	500	2500	-	2125
3580	500	2500	-	3325
3581	500	2500	-	3350
3582	500	2500	-	3375
3583	500	2500	-	3400
3584	500	2500	-	3425
3585	500	2500	-	3450
3586	500	2500	-	3475
3587	500	2500	-	3500
3588	500	2500	-	3525
3589	500	2500	-	3550
3590	500	2500	-	3575
3591	500	2500	-	3600
3592	500	2500	-	3625
3593	500	2500	-	3650
3594	500	2500	-	3675
3595	500	2500	-	3700
3596	500	2500	-	3725
3597	500	2500	-	3750
2671	900	4500	0.6	1710
3244	600	3000	0.8	1710
3473	500	2500	1.4	650
2923	750	3750	1.7	1702
3372	550	2750	2.5	1701
3127	650	3250	2.9	1696
3515	500	2500	3.0	1700
2748	850	4250	3.5	1680
2832	800	4000	3.8	1680
3020	700	3500	4.3	1680
3243	600	3000	5.0	1680
3371	550	2750	5.5	1674
3514	500	2500	6.0	1675

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
2269	1250	35.9	6250	-	2976	35.9
2220	1300	37.4	6500	0.8	2925	35.7
2319	1200	34.5	6000	-	2940	34.5
2320	1200	34.5	6000	-	3000	34.5
2373	1150	33.0	5750	-	2964	33.0
2374	1150	33.0	5750	-	3021	33.0
2375	1150	33.0	5750	-	3078	33.0
2186	1300	37.6	6500	2.3	715	33.0
2187	1300	37.6	6500	2.3	780	33.0
2188	1300	37.6	6500	2.3	845	33.0
2189	1300	37.6	6500	2.3	910	33.0
2190	1300	37.6	6500	2.3	975	33.0
2191	1300	37.6	6500	2.3	1040	33.0
2192	1300	37.6	6500	2.3	1105	33.0
2193	1300	37.6	6500	2.3	1170	33.0
2194	1300	37.6	6500	2.3	1235	33.0
2195	1300	37.6	6500	2.3	1300	33.0
2185	1300	37.6	6500	2.8	650	31.9
2430	1100	31.6	5500	-	2970	31.6
2431	1100	31.6	5500	-	3025	31.6
2432	1100	31.6	5500	-	3080	31.6
2433	1100	31.6	5500	-	3135	31.6
2196	1300	37.6	6500	3.0	1365	31.6
2232	1250	36.1	6250	2.4	682	31.3
2243	1250	36.1	6250	2.4	1364	31.3
2268	1250	34.7	6250	1.8	2914	31.2
2491	1050	30.2	5250	-	2964	30.2
2492	1050	30.2	5250	-	3016	30.2
2493	1050	30.2	5250	-	3068	30.2
2494	1050	30.2	5250	-	3120	30.2
2495	1050	30.2	5250	-	3172	30.2
2281	1200	34.7	6000	2.5	660	29.7
2282	1200	34.7	6000	2.5	720	29.7
2294	1200	34.7	6000	2.5	1440	29.7
2555	1000	28.7	5000	-	2950	28.7
2556	1000	28.7	5000	-	3000	28.7
2557	1000	28.7	5000	-	3050	28.7
2558	1000	28.7	5000	-	3100	28.7
2559	1000	28.7	5000	-	3150	28.7
2560	1000	28.7	5000	-	3200	28.7
2561	1000	28.7	5000	-	3250	28.7
2632	950	28.4	4750	-	3290	28.4
2244	1250	36.1	6250	4.0	1426	28.1
2333	1150	33.2	5750	2.6	684	28.0
2334	1150	33.2	5750	2.6	741	28.0
2335	1150	33.2	5750	2.6	798	28.0
2347	1150	33.2	5750	2.6	1482	28.0
2509	1000	29.4	5000	0.7	650	28.0
2576	950	27.9	4750	-	658	27.9
2577	950	27.9	4750	-	705	27.9
2625	950	27.3	4750	-	2961	27.3
2626	950	27.3	4750	-	3008	27.3
2627	950	27.3	4750	-	3055	27.3
2628	950	27.3	4750	-	3102	27.3
2629	950	27.3	4750	-	3149	27.3
2630	950	27.3	4750	-	3196	27.3
2631	950	27.3	4750	-	3243	27.3
2672	900	26.6	4500	-	1755	26.6
2648	900	26.5	4500	-	675	26.5
2649	900	26.5	4500	-	720	26.5
2650	900	26.5	4500	-	765	26.5
2510	1000	29.4	5000	1.5	700	26.4
2388	1100	31.8	5500	2.7	660	26.3
2389	1100	31.8	5500	2.7	715	26.3
2390	1100	31.8	5500	2.7	770	26.3
2391	1100	31.8	5500	2.7	825	26.3
2404	1100	31.8	5500	2.7	1540	26.3
2706	900	26.2	4500	-	3285	26.2
2699	900	25.9	4500	-	2970	25.9
2700	900	25.9	4500	-	3015	25.9
2701	900	25.9	4500	-	3060	25.9
2702	900	25.9	4500	-	3105	25.9
2703	900	25.9	4500	-	3150	25.9
2704	900	25.9	4500	-	3195	25.9
2705	900	25.9	4500	-	3240	25.9
2372	1150	30.7	5750	2.5	2907	25.7
2751	850	25.1	4250	-	1806	25.1
2724	850	25.0	4250	-	672	25.0
2725	850	25.0	4250	-	714	25.0
2726	850	25.0	4250	-	756	25.0
2727	850	25.0	4250	-	798	25.0
2464	1050	30.3	5250	2.9	1560	24.6
2446	1050	30.9	5250	3.1	624	24.6
2490	1050	29.2	5250	2.3	2912	24.6
2778	850	24.4	4250	-	2940	24.4
2779	850	24.4	4250	-	2982	24.4
2780	850	24.4	4250	-	3024	24.4
2781	850	24.4	4250	-	3066	24.4
2782	850	24.4	4250	-	3108	24.4
2783	850	24.4	4250	-	3150	24.4
2784	850	24.4	4250	-	3192	24.4
2785	850	24.4	4250	-	3234	24.4
2786	850	24.4	4250	-	3276	24.4
2578	950	27.9	4750	1.8	752	24.4
2728	850	25.0	4250	0.6	840	23.8
2836	800	23.7	4000	-	1840	23.7
2175	1300	33.2	6500	4.8	0	23.6
2176	1300	33.2	6500	4.8	65	23.6
2177	1300	33.2	6500	4.8	130	23.6
2178	1300	33.2	6500	4.8	195	23.6
2179	1300	33.2	6500	4.8	260	23.6
2180	1300	33.2	6500	4.8	325	23.6
2529	1000	29.6	5000	3.0	1650	23.6
2807	800	23.5	4000	-	680	23.5
2808	800	23.5	4000	-	720	23.5
2809	800	23.5	4000	-	760	23.5
2810	800	23.5	4000	-	800	23.5
2811	800	23.5	4000	-	840	23.5
2812	800	23.5	4000	-	880	23.5
2598	950	28.1	4750	2.4	1692	23.3
2872	800	23.3	4000	-	3280	23.3
2864	800	23.0	4000	-	2960	23.0
2865	800	23.0	4000	-	3000	23.0
2866	800	23.0	4000	-	3040	23.0
2867	800	23.0	4000	-	3080	23.0
2868	800	23.0	4000	-	3120	23.0
2869	800	23.0	4000	-	3160	23.0
2870	800	23.0	4000	-	3200	23.0
2871	800	23.0	4000	-	3240	23.0
2928	750	22.9	3750	-	1887	22.9
2966	750	22.9	3750	-	3293	22.9
2895	750	22.1	3750	-	666	22.1
2896	750	22.1	3750	-	703	22.1
2897	750	22.1	3750	-	740	22.1
2898	750	22.1	3750	-	777	22.1
2899	750	22.1	3750	-	814	22.1
2900	750	22.1	3750	-	851	22.1
2901	750	22.1	3750	-	888	22.1
2902	750	22.1	3750	-	925	22.1
2222	1250	31.9	6250	5.0	62	22.0
2223	1250	31.9	6250	5.0	124	22.0
2224	1250	31.9	6250	5.0	186	22.0
2225	1250	31.9	6250	5.0	248	22.0
2228	1250	31.9	6250	5.0	434	22.0
2957	750	21.6	3750	-	2960	21.6
2958	750	21.6	3750	-	2997	21.6
2959	750	21.6	3750	-	3034	21.6
2960	750	21.6	3750	-	3071	21.6
2961	750	21.6	3750	-	3108	21.6
2962	750	21.6	3750	-	3145	21.6
2963	750	21.6	3750	-	3182	21.6
2964	750	21.6	3750	-	3219	21.6
2965	750	21.6	3750	-	3256	21.6
2599	950	28.1	4750	3.5	1739	21.2
3066	700	21.0	3500	-	3290	21.0
2651	900	26.5	4500	2.8	810	20.9
2991	700	20.6	3500	-	665	20.6
2992	700	20.6	3500	-	700	20.6
2993	700	20.6	3500	-	735	20.6
2994	700	20.6	3500	-	770	20.6
2995	700	20.6	3500	-	805	20.6
2996	700	20.6	3500	-	840	20.6
2997	700	20.6	3500	-	875	20.6
2998	700	20.6	3500	-	910	20.6
2999	700	20.6	3500	-	945	20.6
3000	700	20.6	3500	-	980	20.6
2647	900	26.5	4500	3.0	630	20.5
3136	650	20.3	3250	-	1984	20.3
3056	700	20.1	3500	-	2940	20.1
3057	700	20.1	3500	-	2975	20.1
3058	700	20.1	3500	-	3010	20.1
3059	700	20.1	3500	-	3045	20.1
3060	700	20.1	3500	-	3080	20.1
3061	700	20.1	3500	-	3115	20.1
3062	700	20.1	3500	-	3150	20.1
3063	700	20.1	3500	-	3185	20.1
3064	700	20.1	3500	-	3220	20.1
3065	700	20.1	3500	-	3255	20.1
3177	650	19.8	3250	-	3296	19.8
3028	700	20.7	3500	0.6	1960	19.6
2806	800	23.5	4000	2.1	640	19.3
2554	1000	26.5	5000	3.6	2900	19.3
3095	650	19.1	3250	-	672	19.1
3096	650	19.1	3250	-	704	19.1
3097	650	19.1	3250	-	736	19.1
3098	650	19.1	3250	-	768	19.1
3099	650	19.1	3250	-	800	19.1
3100	650	19.1	3250	-	832	19.1
3101	650	19.1	3250	-	864	19.1
3102	650	19.1	3250	-	896	19.1
3103	650	19.1	3250	-	928	19.1
3104	650	19.1	3250	-	960	19.1
3105	650	19.1	3250	-	992	19.1
3106	650	19.1	3250	-	1024	19.1
3166	650	18.7	3250	-	2944	18.7
3167	650	18.7	3250	-	2976	18.7
3168	650	18.7	3250	-	3008	18.7
3169	650	18.7	3250	-	3040	18.7
3170	650	18.7	3250	-	3072	18.7
3171	650	18.7	3250	-	3104	18.7
3172	650	18.7	3250	-	3136	18.7
3173	650	18.7	3250	-	3168	18.7
3174	650	18.7	3250	-	3200	18.7
3175	650	18.7	3250	-	3232	18.7
3176	650	18.7	3250	-	3264	18.7
2723	850	25.0	4250	3.2	630	18.6
3297	600	18.3	3000	-	3300	18.3
2956	750	21.6	3750	1.7	2923	18.1
3310	550	17.9	2750	-	27	17.9
3187	600	19.5	3000	0.8	0	17.8
3255	600	17.8	3000	-	2040	17.8
2837	800	23.7	4000	3.0	1880	17.7
3209	600	17.6	3000	-	660	17.6
3210	600	17.6	3000	-	690	17.6
3211	600	17.6	3000	-	720	17.6
3212	600	17.6	3000	-	750	17.6
3213	600	17.6	3000	-	780	17.6
3214	600	17.6	3000	-	810	17.6
3215	600	17.6	3000	-	840	17.6
3216	600	17.6	3000	-	870	17.6
3217	600	17.6	3000	-	900	17.6
3218	600	17.6	3000	-	930	17.6
3219	600	17.6	3000	-	960	17.6
3220	600	17.6	3000	-	990	17.6
3221	600	17.6	3000	-	1020	17.6
3222	600	17.6	3000	-	1050	17.6
3223	600	17.6	3000	-	1080	17.6
2929	750	22.2	3750	2.4	1924	17.4
3285	600	17.2	3000	-	2940	17.2
3286	600	17.2	3000	-	2970	17.2
3287	600	17.2	3000	-	3000	17.2
3288	600	17.2	3000	-	3030	17.2
3289	600	17.2	3000	-	3060	17.2
3290	600	17.2	3000	-	3090	17.2
3291	600	17.2	3000	-	3120	17.2
3292	600	17.2	3000	-	3150	17.2
3293	600	17.2	3000	-	3180	17.2
3294	600	17.2	3000	-	3210	17.2
3295	600	17.2	3000	-	3240	17.2
3296	600	17.2	3000	-	3270	17.2
3351	550	17.2	2750	-	1134	17.2
3386	550	17.2	2750	-	2079	17.2
2673	900	26.6	4500	4.9	1800	16.8
3431	550	16.8	2750	-	3294	16.8
3387	550	16.3	2750	-	2106	16.3
3450	500	16.2	2500	-	75	16.2
3334	550	16.2	2750	-	675	16.2
3335	550	16.2	2750	-	702	16.2
3336	550	16.2	2750	-	729	16.2
3337	550	16.2	2750	-	756	16.2
3338	550	16.2	2750	-	783	16.2
3339	550	16.2	2750	-	810	16.2
3340	550	16.2	2750	-	837	16.2
3341	550	16.2	2750	-	864	16.2
3342	550	16.2	2750	-	891	16.2
3343	550	16.2	2750	-	918	16.2
3344	550	16.2	2750	-	945	16.2
3345	550	16.2	2750	-	972	16.2
3346	550	16.2	2750	-	999	16.2
3347	550	16.2	2750	-	1026	16.2
3348	550	16.2	2750	-	1053	16.2
3349	550	16.2	2750	-	1080	16.2
3350	550	16.2	2750	-	1107	16.2
3137	650	19.2	3250	1.5	2016	16.2
2318	1200	25.2	6000	4.7	2880	15.9
3418	550	15.8	2750	-	2943	15.8
3419	550	15.8	2750	-	2970	15.8
3420	550	15.8	2750	-	2997	15.8
3421	550	15.8	2750	-	3024	15.8
3422	550	15.8	2750	-	3051	15.8
3423	550	15.8	2750	-	3078	15.8
3424	550	15.8	2750	-	3105	15.8
3425	550	15.8	2750	-	3132	15.8
3426	550	15.8	2750	-	3159	15.8
3427	550	15.8	2750	-	3186	15.8
3428	550	15.8	2750	-	3213	15.8
3429	550	15.8	2750	-	3240	15.8
3430	550	15.8	2750	-	3267	15.8
3492	500	15.2	2500	-	1125	15.2
3579	500	15.2	2500	-	3300	15.2
3474	500	15.2	2500	-	675	15.2
3533	500	14.8	2500	-	2150	14.8
3475	500	14.7	2500	-	700	14.7
3476	500	14.7	2500	-	725	14.7
3477	500	14.7	2500	-	750	14.7
3478	500	14.7	2500	-	775	14.7
3479	500	14.7	2500	-	800	14.7
3480	500	14.7	2500	-	825	14.7
3481	500	14.7	2500	-	850	14.7
3482	500	14.7	2500	-	875	14.7
3483	500	14.7	2500	-	900	14.7
3484	500	14.7	2500	-	925	14.7
3485	500	14.7	2500	-	950	14.7
3486	500	14.7	2500	-	975	14.7
3487	500	14.7	2500	-	1000	14.7
3488	500	14.7	2500	-	1025	14.7
3489	500	14.7	2500	-	1050	14.7
3490	500	14.7	2500	-	1075	14.7
3491	500	14.7	2500	-	1100	14.7
2894	750	22.1	3750	3.7	629	14.6
3311	550	17.9	2750	1.6	54	14.6
3565	500	14.4	2500	-	2950	14.4
3566	500	14.4	2500	-	2975	14.4
3567	500	14.4	2500	-	3000	14.4
3568	500	14.4	2500	-	3025	14.4
3569	500	14.4	2500	-	3050	14.4
3570	500	14.4	2500	-	3075	14.4
3571	500	14.4	2500	-	3100	14.4
3572	500	14.4	2500	-	3125	14.4
3573	500	14.4	2500	-	3150	14.4
3574	500	14.4	2500	-	3175	14.4
3575	500	14.4	2500	-	3200	14.4
3576	500	14.4	2500	-	3225	14.4
3577	500	14.4	2500	-	3250	14.4
3578	500	14.4	2500	-	3275	14.4
3451	500	16.2	2500	1.0	100	14.2
3094	650	19.1	3250	2.6	640	13.9
3256	600	17.8	3000	2.3	2070	13.1
3333	550	16.2	2750	1.6	648	12.9
2990	700	20.6	3500	3.9	630	12.9
2777	850	21.7	4250	4.5	2898	12.7
3165	650	18.1	3250	3.7	2912	10.7
3055	700	18.3	3500	4.4	2905	9.5
3208	600	17.6	3000	4.5	630	8.6
3284	600	16.6	3000	4.3	2910	7.9
3534	500	14.8	2500	3.8	2175	7.2

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
2233	1250	36.1	6250	2.4	744	31.3
2234	1250	36.1	6250	2.4	806	31.3
2235	1250	36.1	6250	2.4	868	31.3
2236	1250	36.1	6250	2.4	930	31.3
2237	1250	36.1	6250	2.4	992	31.3
2238	1250	36.1	6250	2.4	1054	31.3
2239	1250	36.1	6250	2.4	1116	31.3
2240	1250	36.1	6250	2.4	1178	31.3
2241	1250	36.1	6250	2.4	1240	31.3
2242	1250	36.1	6250	2.4	1302	31.3
2283	1200	34.7	6000	2.5	780	29.7
2284	1200	34.7	6000	2.5	840	29.7
2285	1200	34.7	6000	2.5	900	29.7
2286	1200	34.7	6000	2.5	960	29.7
2287	1200	34.7	6000	2.5	1020	29.7
2288	1200	34.7	6000	2.5	1080	29.7
2289	1200	34.7	6000	2.5	1140	29.7
2290	1200	34.7	6000	2.5	1200	29.7
2291	1200	34.7	6000	2.5	1260	29.7
2292	1200	34.7	6000	2.5	1320	29.7
2293	1200	34.7	6000	2.5	1380	29.7
2336	1150	33.2	5750	2.6	855	28.0
2337	1150	33.2	5750	2.6	912	28.0
2338	1150	33.2	5750	2.6	969	28.0
2339	1150	33.2	5750	2.6	1026	28.0
2340	1150	33.2	5750	2.6	1083	28.0
2341	1150	33.2	5750	2.6	1140	28.0
2342	1150	33.2	5750	2.6	1197	28.0
2343	1150	33.2	5750	2.6	1254	28.0
2344	1150	33.2	5750	2.6	1311	28.0
2345	1150	33.2	5750	2.6	1368	28.0
2346	1150	33.2	5750	2.6	1425	28.0
2429	1100	31.1	5500	1.9	2915	27.3
2392	1100	31.8	5500	2.7	880	26.3
2393	1100	31.8	5500	2.7	935	26.3
2394	1100	31.8	5500	2.7	990	26.3
2395	1100	31.8	5500	2.7	1045	26.3
2396	1100	31.8	5500	2.7	1100	26.3
2397	1100	31.8	5500	2.7	1155	26.3
2398	1100	31.8	5500	2.7	1210	26.3
2399	1100	31.8	5500	2.7	1265	26.3
2400	1100	31.8	5500	2.7	1320	26.3
2401	1100	31.8	5500	2.7	1375	26.3
2402	1100	31.8	5500	2.7	1430	26.3
2403	1100	31.8	5500	2.7	1485	26.3
2447	1050	30.3	5250	2.9	676	24.6
2448	1050	30.3	5250	2.9	728	24.6
2449	1050	30.3	5250	2.9	780	24.6
2450	1050	30.3	5250	2.9	832	24.6
2451	1050	30.3	5250	2.9	884	24.6
2452	1050	30.3	5250	2.9	936	24.6
2453	1050	30.3	5250	2.9	988	24.6
2454	1050	30.3	5250	2.9	1040	24.6
2455	1050	30.3	5250	2.9	1092	24.6
2456	1050	30.3	5250	2.9	1144	24.6
2457	1050	30.3	5250	2.9	1196	24.6
2458	1050	30.3	5250	2.9	1248	24.6
2459	1050	30.3	5250	2.9	1300	24.6
2460	1050	30.3	5250	2.9	1352	24.6
2461	1050	30.3	5250	2.9	1404	24.6
2462	1050	30.3	5250	2.9	1456	24.6
2463	1050	30.3	5250	2.9	1508	24.6
2465	1050	31.1	5250	3.4	1612	24.2
2597	950	29.9	4750	3.2	1645	23.6
2698	900	25.9	4500	1.2	2925	23.4
2670	900	29.6	4500	3.3	1665	22.9
2511	1000	28.9	5000	3.0	750	22.9
2512	1000	28.9	5000	3.0	800	22.9
2513	1000	28.9	5000	3.0	850	22.9
2514	1000	28.9	5000	3.0	900	22.9
2515	1000	28.9	5000	3.0	950	22.9
2516	1000	28.9	5000	3.0	1000	22.9
2517	1000	28.9	5000	3.0	1050	22.9
2518	1000	28.9	5000	3.0	1100	22.9
2519	1000	28.9	5000	3.0	1150	22.9
2520	1000	28.9	5000	3.0	1200	22.9
2521	1000	28.9	5000	3.0	1250	22.9
2522	1000	28.9	5000	3.0	1300	22.9
2523	1000	28.9	5000	3.0	1350	22.9
2524	1000	28.9	5000	3.0	1400	22.9
2525	1000	28.9	5000	3.0	1450	22.9
2526	1000	28.9	5000	3.0	1500	22.9
2527	1000	28.9	5000	3.0	1550	22.9
2528	1000	28.9	5000	3.0	1600	22.9
2221	1250	31.9	6250	5.0	0	22.0
2226	1250	31.9	6250	5.0	310	22.0
2227	1250	31.9	6250	5.0	372	22.0
2624	950	26.4	4750	2.3	2914	21.8
2669	900	28.1	4500	3.3	1620	21.5
2579	950	27.5	4750	3.2	799	21.1
2580	950	27.5	4750	3.2	846	21.1
2581	950	27.5	4750	3.2	893	21.1
2582	950	27.5	4750	3.2	940	21.1
2583	950	27.5	4750	3.2	987	21.1
2584	950	27.5	4750	3.2	1034	21.1
2585	950	27.5	4750	3.2	1081	21.1
2586	950	27.5	4750	3.2	1128	21.1
2587	950	27.5	4750	3.2	1175	21.1
2588	950	27.5	4750	3.2	1222	21.1
2589	950	27.5	4750	3.2	1269	21.1
2590	950	27.5	4750	3.2	1316	21.1
2591	950	27.5	4750	3.2	1363	21.1
2592	950	27.5	4750	3.2	1410	21.1
2593	950	27.5	4750	3.2	1457	21.1
2594	950	27.5	4750	3.2	1504	21.1
2595	950	27.5	4750	3.2	1551	21.1
2596	950	27.5	4750	3.2	1598	21.1
2747	850	26.7	4250	3.5	1638	19.7
2652	900	26.0	4500	3.3	855	19.3
2653	900	26.0	4500	3.3	900	19.3
2654	900	26.0	4500	3.3	945	19.3
2655	900	26.0	4500	3.3	990	19.3
2656	900	26.0	4500	3.3	1035	19.3
2657	900	26.0	4500	3.3	1080	19.3
2658	900	26.0	4500	3.3	1125	19.3
2659	900	26.0	4500	3.3	1170	19.3
2660	900	26.0	4500	3.3	1215	19.3
2661	900	26.0	4500	3.3	1260	19.3
2662	900	26.0	4500	3.3	1305	19.3
2663	900	26.0	4500	3.3	1350	19.3
2664	900	26.0	4500	3.3	1395	19.3
2665	900	26.0	4500	3.3	1440	19.3
2666	900	26.0	4500	3.3	1485	19.3
2667	900	26.0	4500	3.3	1530	19.3
2668	900	26.0	4500	3.3	1575	19.3
2863	800	23.0	4000	2.0	2920	19.0
2575	950	27.9	4750	4.8	611	18.3
2831	800	25.2	4000	3.8	1640	17.7
2729	850	24.6	4250	3.5	882	17.5
2730	850	24.6	4250	3.5	924	17.5
2731	850	24.6	4250	3.5	966	17.5
2732	850	24.6	4250	3.5	1008	17.5
2733	850	24.6	4250	3.5	1050	17.5
2734	850	24.6	4250	3.5	1092	17.5
2735	850	24.6	4250	3.5	1134	17.5
2736	850	24.6	4250	3.5	1176	17.5
2737	850	24.6	4250	3.5	1218	17.5
2738	850	24.6	4250	3.5	1260	17.5
2739	850	24.6	4250	3.5	1302	17.5
2740	850	24.6	4250	3.5	1344	17.5
2741	850	24.6	4250	3.5	1386	17.5
2742	850	24.6	4250	3.5	1428	17.5
2743	850	24.6	4250	3.5	1470	17.5
2744	850	24.6	4250	3.5	1512	17.5
2745	850	24.6	4250	3.5	1554	17.5
2746	850	24.6	4250	3.5	1596	17.5
2922	750	24.7	3750	4.0	1665	16.7
2813	800	23.1	4000	3.8	920	15.6
2814	800	23.1	4000	3.8	960	15.6
2815	800	23.1	4000	3.8	1000	15.6
2816	800	23.1	4000	3.8	1040	15.6
2817	800	23.1	4000	3.8	1080	15.6
2818	800	23.1	4000	3.8	1120	15.6
2819	800	23.1	4000	3.8	1160	15.6
2820	800	23.1	4000	3.8	1200	15.6
2821	800	23.1	4000	3.8	1240	15.6
2822	800	23.1	4000	3.8	1280	15.6
2823	800	23.1	4000	3.8	1320	15.6
2824	800	23.1	4000	3.8	1360	15.6
2825	800	23.1	4000	3.8	1400	15.6
2826	800	23.1	4000	3.8	1440	15.6
2827	800	23.1	4000	3.8	1480	15.6
2828	800	23.1	4000	3.8	1520	15.6
2829	800	23.1	4000	3.8	1560	15.6
2830	800	23.1	4000	3.8	1600	15.6
2921	750	23.6	3750	4.0	1628	15.6
2752	850	25.1	4250	4.9	1848	15.3
2903	750	22.1	3750	3.6	962	14.9
2904	750	21.7	3750	4.0	999	13.7
2905	750	21.7	3750	4.0	1036	13.7
2906	750	21.7	3750	4.0	1073	13.7
2907	750	21.7	3750	4.0	1110	13.7
2908	750	21.7	3750	4.0	1147	13.7
2909	750	21.7	3750	4.0	1184	13.7
2910	750	21.7	3750	4.0	1221	13.7
2911	750	21.7	3750	4.0	1258	13.7
2912	750	21.7	3750	4.0	1295	13.7
2913	750	21.7	3750	4.0	1332	13.7
2914	750	21.7	3750	4.0	1369	13.7
2915	750	21.7	3750	4.0	1406	13.7
2916	750	21.7	3750	4.0	1443	13.7
2917	750	21.7	3750	4.0	1480	13.7
2918	750	21.7	3750	4.0	1517	13.7
2919	750	21.7	3750	4.0	1554	13.7
2920	750	21.7	3750	4.0	1591	13.7
3019	700	22.0	3500	4.3	1645	13.4
3018	700	21.7	3500	4.3	1610	13.2
3107	650	19.1	3250	3.2	1056	12.7
3126	650	21.4	3250	4.6	1664	12.2
3001	700	20.2	3500	4.3	1015	11.7
3002	700	20.2	3500	4.3	1050	11.7
3003	700	20.2	3500	4.3	1085	11.7
3004	700	20.2	3500	4.3	1120	11.7
3005	700	20.2	3500	4.3	1155	11.7
3006	700	20.2	3500	4.3	1190	11.7
3007	700	20.2	3500	4.3	1225	11.7
3008	700	20.2	3500	4.3	1260	11.7
3009	700	20.2	3500	4.3	1295	11.7
3010	700	20.2	3500	4.3	1330	11.7
3011	700	20.2	3500	4.3	1365	11.7
3012	700	20.2	3500	4.3	1400	11.7
3013	700	20.2	3500	4.3	1435	11.7
3014	700	20.2	3500	4.3	1470	11.7
3015	700	20.2	3500	4.3	1505	11.7
3016	700	20.2	3500	4.3	1540	11.7
3017	700	20.2	3500	4.3	1575	11.7
3125	650	20.4	3250	4.6	1632	11.2
3564	500	14.4	2500	2.2	2925	10.0
3242	600	19.7	3000	5.0	1650	9.7
3108	650	18.8	3250	4.6	1088	9.6
3109	650	18.8	3250	4.6	1120	9.6
3110	650	18.8	3250	4.6	1152	9.6
3111	650	18.8	3250	4.6	1184	9.6
3112	650	18.8	3250	4.6	1216	9.6
3113	650	18.8	3250	4.6	1248	9.6
3114	650	18.8	3250	4.6	1280	9.6
3115	650	18.8	3250	4.6	1312	9.6
3116	650	18.8	3250	4.6	1344	9.6
3117	650	18.8	3250	4.6	1376	9.6
3118	650	18.8	3250	4.6	1408	9.6
3119	650	18.8	3250	4.6	1440	9.6
3120	650	18.8	3250	4.6	1472	9.6
3121	650	18.8	3250	4.6	1504	9.6
3122	650	18.8	3250	4.6	1536	9.6
3123	650	18.8	3250	4.6	1568	9.6
3124	650	18.8	3250	4.6	1600	9.6
3224	600	17.6	3000	4.2	1110	9.3
3495	500	15.1	2500	3.0	1200	9.1
3241	600	18.8	3000	5.0	1620	8.8
3417	550	15.5	2750	3.6	2916	8.3
3225	600	17.3	3000	5.0	1140	7.3
3226	600	17.3	3000	5.0	1170	7.3
3227	600	17.3	3000	5.0	1200	7.3
3228	600	17.3	3000	5.0	1230	7.3
3229	600	17.3	3000	5.0	1260	7.3
3230	600	17.3	3000	5.0	1290	7.3
3231	600	17.3	3000	5.0	1320	7.3
3232	600	17.3	3000	5.0	1350	7.3
3233	600	17.3	3000	5.0	1380	7.3
3234	600	17.3	3000	5.0	1410	7.3
3235	600	17.3	3000	5.0	1440	7.3
3236	600	17.3	3000	5.0	1470	7.3
3237	600	17.3	3000	5.0	1500	7.3
3238	600	17.3	3000	5.0	1530	7.3
3239	600	17.3	3000	5.0	1560	7.3
3240	600	17.3	3000	5.0	1590	7.3
3352	550	16.4	2750	4.7	1161	6.9
3388	550	16.3	2750	4.9	2133	6.5

Overall score = score_ratio - 2 * repetitive DNA content