Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign17 - back to list

Summary
Image
Unique putative probes (253)
Putative probes (523)
Redundant probes (10)

Summary

`./analyse_probe_search_results : Tue Feb 13 12:56:05 2007`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign17
  assembly      : NCBIM36
  bias          : 3prime
  chromosome    : 16
  id            : 4
  strand        : 1
  -------------

Design window length: 3661bp

All jobs for probe design are successful - total: 59
Fetched conf : Exonerate mouse_NCBIM36 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 1179 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1179/1179
Putative probes that are unique                 : 253/1179
Putative probes exceeding selection criteria    : 786/1179 (both unique and not unique)
Putative probes with successfully-picked primers: 501/786

Identified 10 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
3602	1300	6500	-	260
3603	1300	6500	-	325
3639	1250	6250	-	248
3640	1250	6250	-	310
3641	1250	6250	-	372
3678	1200	6000	-	240
3679	1200	6000	-	300
3680	1200	6000	-	360
3681	1200	6000	-	420
3720	1150	5750	-	228
3721	1150	5750	-	285
3722	1150	5750	-	342
3723	1150	5750	-	399
3724	1150	5750	-	456
3765	1100	5500	-	220
3766	1100	5500	-	275
3767	1100	5500	-	330
3768	1100	5500	-	385
3769	1100	5500	-	440
3770	1100	5500	-	495
3813	1050	5250	-	260
3814	1050	5250	-	312
3815	1050	5250	-	364
3816	1050	5250	-	416
3817	1050	5250	-	468
3818	1050	5250	-	520
3819	1050	5250	-	572
3864	1000	5000	-	250
3865	1000	5000	-	300
3866	1000	5000	-	350
3867	1000	5000	-	400
3868	1000	5000	-	450
3869	1000	5000	-	500
3870	1000	5000	-	550
3871	1000	5000	-	600
3918	950	4750	-	235
3919	950	4750	-	282
3920	950	4750	-	329
3921	950	4750	-	376
3922	950	4750	-	423
3923	950	4750	-	470
3924	950	4750	-	517
3925	950	4750	-	564
3926	950	4750	-	611
3927	950	4750	-	658
3976	900	4500	-	225
3977	900	4500	-	270
3978	900	4500	-	315
3979	900	4500	-	360
3980	900	4500	-	405
3981	900	4500	-	450
3982	900	4500	-	495
3983	900	4500	-	540
3984	900	4500	-	585
3985	900	4500	-	630
3986	900	4500	-	675
3987	900	4500	-	720
4039	850	4250	-	252
4040	850	4250	-	294
4041	850	4250	-	336
4042	850	4250	-	378
4043	850	4250	-	420
4044	850	4250	-	462
4045	850	4250	-	504
4046	850	4250	-	546
4047	850	4250	-	588
4048	850	4250	-	630
4049	850	4250	-	672
4050	850	4250	-	714
4051	850	4250	-	756
4106	800	4000	-	240
4107	800	4000	-	280
4108	800	4000	-	320
4109	800	4000	-	360
4110	800	4000	-	400
4111	800	4000	-	440
4112	800	4000	-	480
4113	800	4000	-	520
4114	800	4000	-	560
4115	800	4000	-	600
4116	800	4000	-	640
4117	800	4000	-	680
4118	800	4000	-	720
4119	800	4000	-	760
4120	800	4000	-	800
4121	800	4000	-	840
4178	750	3750	-	222
4179	750	3750	-	259
4180	750	3750	-	296
4181	750	3750	-	333
4182	750	3750	-	370
4183	750	3750	-	407
4184	750	3750	-	444
4185	750	3750	-	481
4186	750	3750	-	518
4187	750	3750	-	555
4188	750	3750	-	592
4189	750	3750	-	629
4190	750	3750	-	666
4191	750	3750	-	703
4192	750	3750	-	740
4193	750	3750	-	777
4194	750	3750	-	814
4195	750	3750	-	851
4196	750	3750	-	888
4258	700	3500	-	245
4259	700	3500	-	280
4260	700	3500	-	315
4261	700	3500	-	350
4262	700	3500	-	385
4263	700	3500	-	420
4264	700	3500	-	455
4265	700	3500	-	490
4266	700	3500	-	525
4267	700	3500	-	560
4268	700	3500	-	595
4269	700	3500	-	630
4270	700	3500	-	665
4271	700	3500	-	700
4272	700	3500	-	735
4273	700	3500	-	770
4274	700	3500	-	805
4275	700	3500	-	840
4276	700	3500	-	875
4277	700	3500	-	910
4343	650	3250	-	224
4344	650	3250	-	256
4345	650	3250	-	288
4346	650	3250	-	320
4347	650	3250	-	352
4348	650	3250	-	384
4349	650	3250	-	416
4350	650	3250	-	448
4351	650	3250	-	480
4352	650	3250	-	512
4353	650	3250	-	544
4354	650	3250	-	576
4355	650	3250	-	608
4356	650	3250	-	640
4357	650	3250	-	672
4358	650	3250	-	704
4359	650	3250	-	736
4360	650	3250	-	768
4361	650	3250	-	800
4362	650	3250	-	832
4363	650	3250	-	864
4364	650	3250	-	896
4365	650	3250	-	928
4366	650	3250	-	960
4439	600	3000	-	240
4440	600	3000	-	270
4441	600	3000	-	300
4442	600	3000	-	330
4443	600	3000	-	360
4444	600	3000	-	390
4445	600	3000	-	420
4446	600	3000	-	450
4447	600	3000	-	480
4448	600	3000	-	510
4449	600	3000	-	540
4450	600	3000	-	570
4451	600	3000	-	600
4452	600	3000	-	630
4453	600	3000	-	660
4454	600	3000	-	690
4455	600	3000	-	720
4456	600	3000	-	750
4457	600	3000	-	780
4458	600	3000	-	810
4459	600	3000	-	840
4460	600	3000	-	870
4461	600	3000	-	900
4462	600	3000	-	930
4463	600	3000	-	960
4464	600	3000	-	990
4465	600	3000	-	1020
4542	550	2750	-	216
4543	550	2750	-	243
4544	550	2750	-	270
4545	550	2750	-	297
4546	550	2750	-	324
4547	550	2750	-	351
4548	550	2750	-	378
4549	550	2750	-	405
4550	550	2750	-	432
4551	550	2750	-	459
4552	550	2750	-	486
4553	550	2750	-	513
4554	550	2750	-	540
4555	550	2750	-	567
4556	550	2750	-	594
4557	550	2750	-	621
4558	550	2750	-	648
4559	550	2750	-	675
4560	550	2750	-	702
4561	550	2750	-	729
4562	550	2750	-	756
4563	550	2750	-	783
4564	550	2750	-	810
4565	550	2750	-	837
4566	550	2750	-	864
4567	550	2750	-	891
4568	550	2750	-	918
4569	550	2750	-	945
4570	550	2750	-	972
4571	550	2750	-	999
4572	550	2750	-	1026
4573	550	2750	-	1053
4574	550	2750	-	1080
4635	550	2750	-	2727
4636	550	2750	-	2754
4659	500	2500	-	225
4660	500	2500	-	250
4661	500	2500	-	275
4662	500	2500	-	300
4663	500	2500	-	325
4664	500	2500	-	350
4665	500	2500	-	375
4666	500	2500	-	400
4667	500	2500	-	425
4668	500	2500	-	450
4669	500	2500	-	475
4670	500	2500	-	500
4671	500	2500	-	525
4672	500	2500	-	550
4673	500	2500	-	575
4674	500	2500	-	600
4675	500	2500	-	625
4676	500	2500	-	650
4677	500	2500	-	675
4678	500	2500	-	700
4679	500	2500	-	725
4680	500	2500	-	750
4681	500	2500	-	775
4682	500	2500	-	800
4683	500	2500	-	825
4684	500	2500	-	850
4685	500	2500	-	875
4686	500	2500	-	900
4687	500	2500	-	925
4688	500	2500	-	950
4689	500	2500	-	975
4690	500	2500	-	1000
4691	500	2500	-	1025
4692	500	2500	-	1050
4693	500	2500	-	1075
4694	500	2500	-	1100
4695	500	2500	-	1125
4759	500	2500	-	2725
4760	500	2500	-	2750
4761	500	2500	-	2775
4762	500	2500	-	2800
4763	500	2500	-	2825

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
3598	1300	41.4	6500	-	0	41.4
3599	1300	41.4	6500	-	65	41.4
3600	1300	41.4	6500	-	130	41.4
3601	1300	41.4	6500	-	195	41.4
3604	1300	40.1	6500	-	390	40.1
3605	1300	40.1	6500	-	455	40.1
3606	1300	40.1	6500	-	520	40.1
3607	1300	40.1	6500	-	585	40.1
3608	1300	40.1	6500	-	650	40.1
3609	1300	40.1	6500	-	715	40.1
3610	1300	40.1	6500	-	780	40.1
3611	1300	40.1	6500	-	845	40.1
3612	1300	40.1	6500	-	910	40.1
3635	1250	39.8	6250	-	0	39.8
3636	1250	39.8	6250	-	62	39.8
3637	1250	39.8	6250	-	124	39.8
3638	1250	39.8	6250	-	186	39.8
3715	1200	38.7	6000	-	2460	38.7
3642	1250	38.6	6250	-	434	38.6
3643	1250	38.6	6250	-	496	38.6
3644	1250	38.6	6250	-	558	38.6
3645	1250	38.6	6250	-	620	38.6
3646	1250	38.6	6250	-	682	38.6
3647	1250	38.6	6250	-	744	38.6
3648	1250	38.6	6250	-	806	38.6
3649	1250	38.6	6250	-	868	38.6
3650	1250	38.6	6250	-	930	38.6
3674	1200	38.2	6000	-	0	38.2
3675	1200	38.2	6000	-	60	38.2
3676	1200	38.2	6000	-	120	38.2
3677	1200	38.2	6000	-	180	38.2
3725	1150	37.3	5750	-	513	37.3
3759	1150	37.1	5750	-	2451	37.1
3760	1150	37.1	5750	-	2508	37.1
3682	1200	37.0	6000	-	480	37.0
3683	1200	37.0	6000	-	540	37.0
3684	1200	37.0	6000	-	600	37.0
3685	1200	37.0	6000	-	660	37.0
3686	1200	37.0	6000	-	720	37.0
3687	1200	37.0	6000	-	780	37.0
3688	1200	37.0	6000	-	840	37.0
3689	1200	37.0	6000	-	900	37.0
3690	1200	37.0	6000	-	960	37.0
3691	1200	37.0	6000	0.2	1020	36.7
3716	1150	36.6	5750	-	0	36.6
3717	1150	36.6	5750	-	57	36.6
3718	1150	36.6	5750	-	114	36.6
3719	1150	36.6	5750	-	171	36.6
3771	1100	35.7	5500	-	550	35.7
3726	1150	35.5	5750	-	570	35.5
3727	1150	35.5	5750	-	627	35.5
3728	1150	35.5	5750	-	684	35.5
3729	1150	35.5	5750	-	741	35.5
3730	1150	35.5	5750	-	798	35.5
3731	1150	35.5	5750	-	855	35.5
3732	1150	35.5	5750	-	912	35.5
3733	1150	35.5	5750	-	969	35.5
3734	1150	35.5	5750	-	1026	35.5
3806	1100	35.5	5500	-	2475	35.5
3807	1100	35.5	5500	-	2530	35.5
3805	1100	35.5	5500	0.1	2420	35.3
3714	1200	38.7	6000	1.8	2400	35.2
3761	1100	35.0	5500	-	0	35.0
3762	1100	35.0	5500	-	55	35.0
3763	1100	35.0	5500	-	110	35.0
3764	1100	35.0	5500	-	165	35.0
3812	1050	34.3	5250	-	208	34.3
3772	1100	34.0	5500	-	605	34.0
3773	1100	34.0	5500	-	660	34.0
3774	1100	34.0	5500	-	715	34.0
3775	1100	34.0	5500	-	770	34.0
3776	1100	34.0	5500	-	825	34.0
3777	1100	34.0	5500	-	880	34.0
3778	1100	34.0	5500	-	935	34.0
3779	1100	34.0	5500	-	990	34.0
3780	1100	34.0	5500	-	1045	34.0
3781	1100	34.0	5500	-	1100	34.0
3855	1050	33.9	5250	-	2444	33.9
3856	1050	33.9	5250	-	2496	33.9
3857	1050	33.9	5250	-	2548	33.9
3858	1050	33.9	5250	-	2600	33.9
3808	1050	33.4	5250	-	0	33.4
3809	1050	33.4	5250	-	52	33.4
3810	1050	33.4	5250	-	104	33.4
3811	1050	33.4	5250	-	156	33.4
3735	1150	35.5	5750	1.3	1083	32.9
3872	1000	32.5	5000	-	650	32.5
3820	1050	32.4	5250	-	624	32.4
3821	1050	32.4	5250	-	676	32.4
3822	1050	32.4	5250	-	728	32.4
3823	1050	32.4	5250	-	780	32.4
3824	1050	32.4	5250	-	832	32.4
3825	1050	32.4	5250	-	884	32.4
3826	1050	32.4	5250	-	936	32.4
3827	1050	32.4	5250	-	988	32.4
3828	1050	32.4	5250	-	1040	32.4
3829	1050	32.4	5250	-	1092	32.4
3830	1050	32.4	5250	-	1144	32.4
3758	1150	37.1	5750	2.3	2394	32.4
3908	1000	32.3	5000	-	2450	32.3
3909	1000	32.3	5000	-	2500	32.3
3910	1000	32.3	5000	-	2550	32.3
3911	1000	32.3	5000	-	2600	32.3
3912	1000	32.3	5000	-	2650	32.3
3859	1000	31.8	5000	-	0	31.8
3860	1000	31.8	5000	-	50	31.8
3861	1000	31.8	5000	-	100	31.8
3862	1000	31.8	5000	-	150	31.8
3863	1000	31.8	5000	-	200	31.8
3651	1250	34.9	6250	1.9	992	31.1
3873	1000	30.9	5000	-	700	30.9
3874	1000	30.9	5000	-	750	30.9
3875	1000	30.9	5000	-	800	30.9
3876	1000	30.9	5000	-	850	30.9
3877	1000	30.9	5000	-	900	30.9
3878	1000	30.9	5000	-	950	30.9
3879	1000	30.9	5000	-	1000	30.9
3880	1000	30.9	5000	-	1050	30.9
3881	1000	30.9	5000	-	1100	30.9
3882	1000	30.9	5000	-	1150	30.9
3883	1000	30.9	5000	-	1200	30.9
3928	950	30.8	4750	-	705	30.8
3965	950	30.6	4750	-	2444	30.6
3966	950	30.6	4750	-	2491	30.6
3967	950	30.6	4750	-	2538	30.6
3968	950	30.6	4750	-	2585	30.6
3969	950	30.6	4750	-	2632	30.6
3970	950	30.6	4750	-	2679	30.6
3913	950	30.3	4750	-	0	30.3
3914	950	30.3	4750	-	47	30.3
3915	950	30.3	4750	-	94	30.3
3916	950	30.3	4750	-	141	30.3
3917	950	30.3	4750	-	188	30.3
4032	900	29.8	4500	-	2745	29.8
3929	950	29.3	4750	-	752	29.3
3930	950	29.3	4750	-	799	29.3
3931	950	29.3	4750	-	846	29.3
3932	950	29.3	4750	-	893	29.3
3933	950	29.3	4750	-	940	29.3
3934	950	29.3	4750	-	987	29.3
3935	950	29.3	4750	-	1034	29.3
3936	950	29.3	4750	-	1081	29.3
3937	950	29.3	4750	-	1128	29.3
3938	950	29.3	4750	-	1175	29.3
3939	950	29.3	4750	-	1222	29.3
3988	900	29.2	4500	-	765	29.2
3940	950	29.3	4750	0.1	1269	29.1
4025	900	29.0	4500	-	2430	29.0
4026	900	29.0	4500	-	2475	29.0
4027	900	29.0	4500	-	2520	29.0
4028	900	29.0	4500	-	2565	29.0
4029	900	29.0	4500	-	2610	29.0
4030	900	29.0	4500	-	2655	29.0
4031	900	29.0	4500	-	2700	29.0
3971	900	28.7	4500	-	0	28.7
3972	900	28.7	4500	-	45	28.7
3973	900	28.7	4500	-	90	28.7
3974	900	28.7	4500	-	135	28.7
3975	900	28.7	4500	-	180	28.7
3854	1050	33.9	5250	2.8	2392	28.3
4098	850	28.1	4250	-	2730	28.1
4099	850	28.1	4250	-	2772	28.1
4038	850	27.8	4250	-	210	27.8
3989	900	27.8	4500	-	810	27.8
3990	900	27.8	4500	-	855	27.8
3991	900	27.8	4500	-	900	27.8
3992	900	27.8	4500	-	945	27.8
3993	900	27.8	4500	-	990	27.8
3994	900	27.8	4500	-	1035	27.8
3995	900	27.8	4500	-	1080	27.8
3996	900	27.8	4500	-	1125	27.8
3997	900	27.8	4500	-	1170	27.8
3998	900	27.8	4500	-	1215	27.8
3999	900	27.8	4500	-	1260	27.8
4000	900	27.8	4500	-	1305	27.8
4052	850	27.6	4250	-	798	27.6
4091	850	27.4	4250	-	2436	27.4
4092	850	27.4	4250	-	2478	27.4
4093	850	27.4	4250	-	2520	27.4
4094	850	27.4	4250	-	2562	27.4
4095	850	27.4	4250	-	2604	27.4
4096	850	27.4	4250	-	2646	27.4
4097	850	27.4	4250	-	2688	27.4
4033	850	27.1	4250	-	0	27.1
4034	850	27.1	4250	-	42	27.1
4035	850	27.1	4250	-	84	27.1
4036	850	27.1	4250	-	126	27.1
4037	850	27.1	4250	-	168	27.1
4168	800	26.5	4000	-	2720	26.5
4169	800	26.5	4000	-	2760	26.5
4170	800	26.5	4000	-	2800	26.5
4171	800	26.5	4000	-	2840	26.5
4053	850	26.2	4250	-	840	26.2
4054	850	26.2	4250	-	882	26.2
4055	850	26.2	4250	-	924	26.2
4056	850	26.2	4250	-	966	26.2
4057	850	26.2	4250	-	1008	26.2
4058	850	26.2	4250	-	1050	26.2
4059	850	26.2	4250	-	1092	26.2
4060	850	26.2	4250	-	1134	26.2
4061	850	26.2	4250	-	1176	26.2
4062	850	26.2	4250	-	1218	26.2
4063	850	26.2	4250	-	1260	26.2
4064	850	26.2	4250	-	1302	26.2
4065	850	26.2	4250	-	1344	26.2
4161	800	25.8	4000	-	2440	25.8
4162	800	25.8	4000	-	2480	25.8
4163	800	25.8	4000	-	2520	25.8
4164	800	25.8	4000	-	2560	25.8
4165	800	25.8	4000	-	2600	25.8
4166	800	25.8	4000	-	2640	25.8
4167	800	25.8	4000	-	2680	25.8
4100	800	25.5	4000	-	0	25.5
4101	800	25.5	4000	-	40	25.5
4102	800	25.5	4000	-	80	25.5
4103	800	25.5	4000	-	120	25.5
4104	800	25.5	4000	-	160	25.5
4105	800	25.5	4000	-	200	25.5
4246	750	24.8	3750	-	2738	24.8
4247	750	24.8	3750	-	2775	24.8
4248	750	24.8	3750	-	2812	24.8
4249	750	24.8	3750	-	2849	24.8
4250	750	24.8	3750	-	2886	24.8
4122	800	24.7	4000	-	880	24.7
4123	800	24.7	4000	-	920	24.7
4124	800	24.7	4000	-	960	24.7
4125	800	24.7	4000	-	1000	24.7
4126	800	24.7	4000	-	1040	24.7
4127	800	24.7	4000	-	1080	24.7
4128	800	24.7	4000	-	1120	24.7
4129	800	24.7	4000	-	1160	24.7
4130	800	24.7	4000	-	1200	24.7
4131	800	24.7	4000	-	1240	24.7
4132	800	24.7	4000	-	1280	24.7
4133	800	24.7	4000	-	1320	24.7
4134	800	24.7	4000	-	1360	24.7
4135	800	24.7	4000	-	1400	24.7
4238	750	24.2	3750	-	2442	24.2
4239	750	24.2	3750	-	2479	24.2
4240	750	24.2	3750	-	2516	24.2
4241	750	24.2	3750	-	2553	24.2
4242	750	24.2	3750	-	2590	24.2
4243	750	24.2	3750	-	2627	24.2
4244	750	24.2	3750	-	2664	24.2
4245	750	24.2	3750	-	2701	24.2
4172	750	23.9	3750	-	0	23.9
4173	750	23.9	3750	-	37	23.9
4174	750	23.9	3750	-	74	23.9
4175	750	23.9	3750	-	111	23.9
4176	750	23.9	3750	-	148	23.9
4177	750	23.9	3750	-	185	23.9
3831	1050	29.0	5250	2.7	1196	23.7
4329	700	23.2	3500	-	2730	23.2
4330	700	23.2	3500	-	2765	23.2
4331	700	23.2	3500	-	2800	23.2
4332	700	23.2	3500	-	2835	23.2
4333	700	23.2	3500	-	2870	23.2
4334	700	23.2	3500	-	2905	23.2
4335	700	23.2	3500	-	2940	23.2
4197	750	23.1	3750	-	925	23.1
4198	750	23.1	3750	-	962	23.1
4199	750	23.1	3750	-	999	23.1
4200	750	23.1	3750	-	1036	23.1
4201	750	23.1	3750	-	1073	23.1
4202	750	23.1	3750	-	1110	23.1
4203	750	23.1	3750	-	1147	23.1
4204	750	23.1	3750	-	1184	23.1
4205	750	23.1	3750	-	1221	23.1
4206	750	23.1	3750	-	1258	23.1
4207	750	23.1	3750	-	1295	23.1
4208	750	23.1	3750	-	1332	23.1
4209	750	23.1	3750	-	1369	23.1
4210	750	23.1	3750	-	1406	23.1
4211	750	23.1	3750	-	1443	23.1
4257	700	22.9	3500	-	210	22.9
4278	700	22.7	3500	-	945	22.7
4321	700	22.6	3500	-	2450	22.6
4322	700	22.6	3500	-	2485	22.6
4323	700	22.6	3500	-	2520	22.6
4324	700	22.6	3500	-	2555	22.6
4325	700	22.6	3500	-	2590	22.6
4326	700	22.6	3500	-	2625	22.6
4327	700	22.6	3500	-	2660	22.6
4328	700	22.6	3500	-	2695	22.6
4251	700	22.3	3500	-	0	22.3
4252	700	22.3	3500	-	35	22.3
4253	700	22.3	3500	-	70	22.3
4254	700	22.3	3500	-	105	22.3
4255	700	22.3	3500	-	140	22.3
4256	700	22.3	3500	-	175	22.3
4066	850	26.2	4250	2.1	1386	22.0
3782	1100	28.5	5500	3.4	1155	21.8
4279	700	21.6	3500	-	980	21.6
4280	700	21.6	3500	-	1015	21.6
4281	700	21.6	3500	-	1050	21.6
4282	700	21.6	3500	-	1085	21.6
4283	700	21.6	3500	-	1120	21.6
4284	700	21.6	3500	-	1155	21.6
4285	700	21.6	3500	-	1190	21.6
4286	700	21.6	3500	-	1225	21.6
4287	700	21.6	3500	-	1260	21.6
4288	700	21.6	3500	-	1295	21.6
4289	700	21.6	3500	-	1330	21.6
4290	700	21.6	3500	-	1365	21.6
4291	700	21.6	3500	-	1400	21.6
4292	700	21.6	3500	-	1435	21.6
4293	700	21.6	3500	-	1470	21.6
4294	700	21.6	3500	-	1505	21.6
4421	650	21.5	3250	-	2720	21.5
4422	650	21.5	3250	-	2752	21.5
4423	650	21.5	3250	-	2784	21.5
4424	650	21.5	3250	-	2816	21.5
4425	650	21.5	3250	-	2848	21.5
4426	650	21.5	3250	-	2880	21.5
4427	650	21.5	3250	-	2912	21.5
4428	650	21.5	3250	-	2944	21.5
4429	650	21.5	3250	-	2976	21.5
4430	650	21.5	3250	-	3008	21.5
3884	1000	27.6	5000	3.2	1250	21.2
4367	650	21.1	3250	-	992	21.1
4412	650	21.0	3250	-	2432	21.0
4413	650	21.0	3250	-	2464	21.0
4414	650	21.0	3250	-	2496	21.0
4415	650	21.0	3250	-	2528	21.0
4416	650	21.0	3250	-	2560	21.0
4417	650	21.0	3250	-	2592	21.0
4418	650	21.0	3250	-	2624	21.0
4419	650	21.0	3250	-	2656	21.0
4420	650	21.0	3250	-	2688	21.0
4320	700	22.6	3500	0.9	2415	20.9
4336	650	20.7	3250	-	0	20.7
4337	650	20.7	3250	-	32	20.7
4338	650	20.7	3250	-	64	20.7
4339	650	20.7	3250	-	96	20.7
4340	650	20.7	3250	-	128	20.7
4341	650	20.7	3250	-	160	20.7
4342	650	20.7	3250	-	192	20.7
4368	650	20.1	3250	-	1024	20.1
4369	650	20.1	3250	-	1056	20.1
4370	650	20.1	3250	-	1088	20.1
4371	650	20.1	3250	-	1120	20.1
4372	650	20.1	3250	-	1152	20.1
4373	650	20.1	3250	-	1184	20.1
4374	650	20.1	3250	-	1216	20.1
4375	650	20.1	3250	-	1248	20.1
4376	650	20.1	3250	-	1280	20.1
4377	650	20.1	3250	-	1312	20.1
4378	650	20.1	3250	-	1344	20.1
4379	650	20.1	3250	-	1376	20.1
4380	650	20.1	3250	-	1408	20.1
4381	650	20.1	3250	-	1440	20.1
4382	650	20.1	3250	-	1472	20.1
4383	650	20.1	3250	-	1504	20.1
4384	650	20.1	3250	-	1536	20.1
4385	650	20.1	3250	-	1568	20.1
4212	750	23.1	3750	1.6	1480	19.9
4522	600	19.9	3000	-	2730	19.9
4523	600	19.9	3000	-	2760	19.9
4524	600	19.9	3000	-	2790	19.9
4525	600	19.9	3000	-	2820	19.9
4526	600	19.9	3000	-	2850	19.9
4527	600	19.9	3000	-	2880	19.9
4528	600	19.9	3000	-	2910	19.9
4529	600	19.9	3000	-	2940	19.9
4530	600	19.9	3000	-	2970	19.9
4531	600	19.9	3000	-	3000	19.9
4532	600	19.9	3000	-	3030	19.9
4533	600	19.9	3000	-	3060	19.9
4438	600	19.6	3000	-	210	19.6
4024	900	27.6	4500	4.0	2385	19.6
4466	600	19.5	3000	-	1050	19.5
4512	600	19.4	3000	-	2430	19.4
4513	600	19.4	3000	-	2460	19.4
4514	600	19.4	3000	-	2490	19.4
4515	600	19.4	3000	-	2520	19.4
4516	600	19.4	3000	-	2550	19.4
4517	600	19.4	3000	-	2580	19.4
4518	600	19.4	3000	-	2610	19.4
4519	600	19.4	3000	-	2640	19.4
4520	600	19.4	3000	-	2670	19.4
4521	600	19.4	3000	-	2700	19.4
4431	600	19.1	3000	-	0	19.1
4432	600	19.1	3000	-	30	19.1
4433	600	19.1	3000	-	60	19.1
4434	600	19.1	3000	-	90	19.1
4435	600	19.1	3000	-	120	19.1
4436	600	19.1	3000	-	150	19.1
4437	600	19.1	3000	-	180	19.1
4485	600	19.5	3000	0.3	1620	18.8
4467	600	18.5	3000	-	1080	18.5
4468	600	18.5	3000	-	1110	18.5
4469	600	18.5	3000	-	1140	18.5
4470	600	18.5	3000	-	1170	18.5
4471	600	18.5	3000	-	1200	18.5
4472	600	18.5	3000	-	1230	18.5
4473	600	18.5	3000	-	1260	18.5
4474	600	18.5	3000	-	1290	18.5
4475	600	18.5	3000	-	1320	18.5
4476	600	18.5	3000	-	1350	18.5
4477	600	18.5	3000	-	1380	18.5
4478	600	18.5	3000	-	1410	18.5
4479	600	18.5	3000	-	1440	18.5
4480	600	18.5	3000	-	1470	18.5
4481	600	18.5	3000	-	1500	18.5
4482	600	18.5	3000	-	1530	18.5
4483	600	18.5	3000	-	1560	18.5
4484	600	18.5	3000	-	1590	18.5
4637	550	18.2	2750	-	2781	18.2
4638	550	18.2	2750	-	2808	18.2
4639	550	18.2	2750	-	2835	18.2
4640	550	18.2	2750	-	2862	18.2
4641	550	18.2	2750	-	2889	18.2
4642	550	18.2	2750	-	2916	18.2
4643	550	18.2	2750	-	2943	18.2
4644	550	18.2	2750	-	2970	18.2
4645	550	18.2	2750	-	2997	18.2
4646	550	18.2	2750	-	3024	18.2
4647	550	18.2	2750	-	3051	18.2
4648	550	18.2	2750	-	3078	18.2
4649	550	18.2	2750	-	3105	18.2
4575	550	17.9	2750	-	1107	17.9
4594	550	17.9	2750	-	1620	17.9
4595	550	17.9	2750	-	1647	17.9
4001	900	24.9	4500	3.6	1350	17.8
4624	550	17.7	2750	-	2430	17.7
4625	550	17.7	2750	-	2457	17.7
4626	550	17.7	2750	-	2484	17.7
4627	550	17.7	2750	-	2511	17.7
4628	550	17.7	2750	-	2538	17.7
4629	550	17.7	2750	-	2565	17.7
4630	550	17.7	2750	-	2592	17.7
4631	550	17.7	2750	-	2619	17.7
4632	550	17.7	2750	-	2646	17.7
4633	550	17.7	2750	-	2673	17.7
4634	550	17.7	2750	-	2700	17.7
4534	550	17.5	2750	-	0	17.5
4535	550	17.5	2750	-	27	17.5
4536	550	17.5	2750	-	54	17.5
4537	550	17.5	2750	-	81	17.5
4538	550	17.5	2750	-	108	17.5
4539	550	17.5	2750	-	135	17.5
4540	550	17.5	2750	-	162	17.5
4541	550	17.5	2750	-	189	17.5
4136	800	23.0	4000	2.8	1440	17.5
4576	550	17.0	2750	-	1134	17.0
4577	550	17.0	2750	-	1161	17.0
4578	550	17.0	2750	-	1188	17.0
4579	550	17.0	2750	-	1215	17.0
4580	550	17.0	2750	-	1242	17.0
4581	550	17.0	2750	-	1269	17.0
4582	550	17.0	2750	-	1296	17.0
4583	550	17.0	2750	-	1323	17.0
4584	550	17.0	2750	-	1350	17.0
4585	550	17.0	2750	-	1377	17.0
4586	550	17.0	2750	-	1404	17.0
4587	550	17.0	2750	-	1431	17.0
4588	550	17.0	2750	-	1458	17.0
4589	550	17.0	2750	-	1485	17.0
4590	550	17.0	2750	-	1512	17.0
4591	550	17.0	2750	-	1539	17.0
4592	550	17.0	2750	-	1566	17.0
4593	550	17.0	2750	-	1593	17.0
4764	500	16.6	2500	-	2850	16.6
4765	500	16.6	2500	-	2875	16.6
4766	500	16.6	2500	-	2900	16.6
4767	500	16.6	2500	-	2925	16.6
4768	500	16.6	2500	-	2950	16.6
4769	500	16.6	2500	-	2975	16.6
4770	500	16.6	2500	-	3000	16.6
4771	500	16.6	2500	-	3025	16.6
4772	500	16.6	2500	-	3050	16.6
4773	500	16.6	2500	-	3075	16.6
4774	500	16.6	2500	-	3100	16.6
4775	500	16.6	2500	-	3125	16.6
4776	500	16.6	2500	-	3150	16.6
4696	500	16.2	2500	-	1150	16.2
4714	500	16.2	2500	-	1600	16.2
4715	500	16.2	2500	-	1625	16.2
4716	500	16.2	2500	-	1650	16.2
4717	500	16.2	2500	-	1675	16.2
4718	500	16.2	2500	-	1700	16.2
4747	500	16.1	2500	-	2425	16.1
4748	500	16.1	2500	-	2450	16.1
4749	500	16.1	2500	-	2475	16.1
4750	500	16.1	2500	-	2500	16.1
4751	500	16.1	2500	-	2525	16.1
4752	500	16.1	2500	-	2550	16.1
4753	500	16.1	2500	-	2575	16.1
4754	500	16.1	2500	-	2600	16.1
4755	500	16.1	2500	-	2625	16.1
4756	500	16.1	2500	-	2650	16.1
4757	500	16.1	2500	-	2675	16.1
4758	500	16.1	2500	-	2700	16.1
4650	500	15.9	2500	-	0	15.9
4651	500	15.9	2500	-	25	15.9
4652	500	15.9	2500	-	50	15.9
4653	500	15.9	2500	-	75	15.9
4654	500	15.9	2500	-	100	15.9
4655	500	15.9	2500	-	125	15.9
4656	500	15.9	2500	-	150	15.9
4657	500	15.9	2500	-	175	15.9
4658	500	15.9	2500	-	200	15.9
4596	550	17.9	2750	1.1	1674	15.7
4697	500	15.4	2500	-	1175	15.4
4698	500	15.4	2500	-	1200	15.4
4699	500	15.4	2500	-	1225	15.4
4700	500	15.4	2500	-	1250	15.4
4701	500	15.4	2500	-	1275	15.4
4702	500	15.4	2500	-	1300	15.4
4703	500	15.4	2500	-	1325	15.4
4704	500	15.4	2500	-	1350	15.4
4705	500	15.4	2500	-	1375	15.4
4706	500	15.4	2500	-	1400	15.4
4707	500	15.4	2500	-	1425	15.4
4708	500	15.4	2500	-	1450	15.4
4709	500	15.4	2500	-	1475	15.4
4710	500	15.4	2500	-	1500	15.4
4711	500	15.4	2500	-	1525	15.4
4712	500	15.4	2500	-	1550	15.4
4713	500	15.4	2500	-	1575	15.4
3613	1300	23.2	6500	4.4	975	14.4
4295	700	20.1	3500	3.1	1540	13.8
4719	500	16.2	2500	1.4	1725	13.4

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
3907	1000	32.3	5000	2.1	2400	28.1
3964	950	30.6	4750	2.5	2397	25.6
4090	850	27.4	4250	3.2	2394	21.1
4160	800	25.8	4000	2.6	2400	20.6
4237	750	24.2	3750	2.1	2405	19.9
4411	650	21.0	3250	3.2	2400	14.5
4511	600	19.4	3000	3.5	2400	12.4
4623	550	17.7	2750	3.3	2403	11.2
4386	650	18.0	3250	4.9	1600	8.1
4746	500	16.1	2500	4.2	2400	7.7

Overall score = score_ratio - 2 * repetitive DNA content