Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign20 - back to list

Summary
Image
Unique putative probes (49)
Putative probes (231)
Redundant probes (301)

Summary

`./analyse_probe_search_results : Tue Dec 19 12:00:58 2006`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign20
  assembly      : NCBIM36
  bias          : 5prime
  chromosome    : 16
  id            : 2
  strand        : 1
  -------------

Design window length: 3171bp

All jobs for probe design are successful - total: 49
Fetched conf : Exonerate mouse_NCBIM36 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM36/genome/softmasked_dusted
Fetched 974 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 974/974
Putative probes that are unique                 : 49/974
Putative probes exceeding selection criteria    : 581/974 (both unique and not unique)
Putative probes with successfully-picked primers: 500/581

Identified 301 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
1770	700	3500	-	1260
1771	700	3500	-	1295
1808	650	3250	-	96
1809	650	3250	-	128
1845	650	3250	-	1280
1846	650	3250	-	1312
1847	650	3250	-	1344
1887	600	3000	-	90
1888	600	3000	-	120
1889	600	3000	-	150
1890	600	3000	-	180
1926	600	3000	-	1260
1927	600	3000	-	1290
1928	600	3000	-	1320
1929	600	3000	-	1350
1930	600	3000	-	1380
1973	550	2750	-	81
1974	550	2750	-	108
1975	550	2750	-	135
1976	550	2750	-	162
1977	550	2750	-	189
1978	550	2750	-	216
2017	550	2750	-	1269
2018	550	2750	-	1296
2019	550	2750	-	1323
2020	550	2750	-	1350
2021	550	2750	-	1377
2022	550	2750	-	1404
2023	550	2750	-	1431
2072	500	2500	-	100
2073	500	2500	-	125
2074	500	2500	-	150
2075	500	2500	-	175
2076	500	2500	-	200
2077	500	2500	-	225
2078	500	2500	-	250
2079	500	2500	-	275
2118	500	2500	-	1250
2119	500	2500	-	1275
2120	500	2500	-	1300
2121	500	2500	-	1325
2122	500	2500	-	1350
2123	500	2500	-	1375
2124	500	2500	-	1400
2125	500	2500	-	1425
2126	500	2500	-	1450
2127	500	2500	-	1475
2128	500	2500	-	1500
2174	500	2500	-	2650

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
1455	950	28.8	4750	-	94	28.8
1503	900	28.5	4500	-	90	28.5
1504	900	27.3	4500	-	135	27.3
1505	900	27.3	4500	0.1	180	27.1
1554	850	26.9	4250	-	84	26.9
1555	850	26.9	4250	-	126	26.9
1556	850	26.9	4250	-	168	26.9
1410	1000	26.3	5000	-	50	26.3
1456	950	28.8	4750	1.3	141	26.3
1368	1050	26.0	5250	-	0	26.0
1201	1300	29.0	6500	1.5	0	25.9
1202	1300	29.0	6500	1.5	65	25.9
1203	1300	29.0	6500	1.5	130	25.9
1204	1300	29.0	6500	1.5	195	25.9
1205	1300	29.0	6500	1.5	260	25.9
1206	1300	29.0	6500	1.5	325	25.9
1207	1300	29.0	6500	1.5	390	25.9
1208	1300	29.0	6500	1.5	455	25.9
1209	1300	29.0	6500	1.5	520	25.9
1210	1300	29.0	6500	1.5	585	25.9
1211	1300	29.0	6500	1.5	650	25.9
1212	1300	29.0	6500	1.5	715	25.9
1557	850	25.8	4250	-	210	25.8
1610	800	25.3	4000	-	80	25.3
1611	800	25.3	4000	-	120	25.3
1612	800	25.3	4000	-	160	25.3
1613	800	25.3	4000	-	200	25.3
1454	950	25.0	4750	-	47	25.0
1409	1000	24.8	5000	-	0	24.8
1231	1250	27.9	6250	1.6	62	24.7
1232	1250	27.9	6250	1.6	124	24.7
1233	1250	27.9	6250	1.6	186	24.7
1234	1250	27.9	6250	1.6	248	24.7
1614	800	24.2	4000	-	240	24.2
1702	750	24.0	3750	-	1258	24.0
1615	800	24.2	4000	0.1	280	24.0
1670	750	23.7	3750	-	74	23.7
1671	750	23.7	3750	-	111	23.7
1672	750	23.7	3750	-	148	23.7
1673	750	23.7	3750	-	185	23.7
1674	750	23.7	3750	-	222	23.7
1675	750	23.7	3750	-	259	23.7
1453	950	23.5	4750	-	0	23.5
1502	900	23.4	4500	-	45	23.4
1676	750	22.7	3750	-	296	22.7
1501	900	22.3	4500	-	0	22.3
1737	700	22.2	3500	-	105	22.2
1738	700	22.2	3500	-	140	22.2
1739	700	22.2	3500	-	175	22.2
1740	700	22.2	3500	-	210	22.2
1741	700	22.2	3500	-	245	22.2
1742	700	22.2	3500	-	280	22.2
1743	700	22.2	3500	-	315	22.2
1553	850	22.1	4250	-	42	22.1
1736	700	21.7	3500	-	70	21.7
1844	650	21.7	3250	-	1248	21.7
1677	750	22.7	3750	0.5	333	21.7
1744	700	21.2	3500	-	350	21.2
1552	850	21.0	4250	-	0	21.0
1810	650	20.6	3250	-	160	20.6
1811	650	20.6	3250	-	192	20.6
1812	650	20.6	3250	-	224	20.6
1813	650	20.6	3250	-	256	20.6
1814	650	20.6	3250	-	288	20.6
1815	650	20.6	3250	-	320	20.6
1816	650	20.6	3250	-	352	20.6
1609	800	20.4	4000	-	40	20.4
1733	750	20.3	3750	-	2405	20.3
1526	900	20.1	4500	-	1125	20.1
1608	800	19.8	4000	-	0	19.8
1817	650	19.7	3250	-	384	19.7
1818	650	19.7	3250	-	416	19.7
1745	700	21.2	3500	0.9	385	19.5
1931	600	19.2	3000	-	1410	19.2
1669	750	19.1	3750	-	37	19.1
1891	600	19.0	3000	-	210	19.0
1892	600	19.0	3000	-	240	19.0
1893	600	19.0	3000	-	270	19.0
1894	600	19.0	3000	-	300	19.0
1895	600	19.0	3000	-	330	19.0
1896	600	19.0	3000	-	360	19.0
1897	600	19.0	3000	-	390	19.0
1898	600	19.0	3000	-	420	19.0
1579	850	19.0	4250	-	1134	19.0
1580	850	19.0	4250	-	1176	19.0
1803	700	18.9	3500	-	2415	18.9
1804	700	18.9	3500	-	2450	18.9
1668	750	18.6	3750	-	0	18.6
1213	1300	21.6	6500	1.5	780	18.5
1807	650	18.3	3250	-	64	18.3
1899	600	18.2	3000	-	450	18.2
1979	550	18.1	2750	-	243	18.1
1735	700	17.9	3500	-	35	17.9
1636	800	17.9	4000	-	1120	17.9
1637	800	17.9	4000	-	1160	17.9
1638	800	17.9	4000	-	1200	17.9
1900	600	18.2	3000	0.2	480	17.8
1802	700	18.9	3500	0.6	2380	17.8
1769	700	17.8	3500	-	1225	17.8
2024	550	17.6	2750	-	1458	17.6
1880	650	17.6	3250	-	2400	17.6
1881	650	17.6	3250	-	2432	17.6
1882	650	17.6	3250	-	2464	17.6
1883	650	17.6	3250	-	2496	17.6
1980	550	17.4	2750	-	270	17.4
1981	550	17.4	2750	-	297	17.4
1982	550	17.4	2750	-	324	17.4
1983	550	17.4	2750	-	351	17.4
1984	550	17.4	2750	-	378	17.4
1985	550	17.4	2750	-	405	17.4
1986	550	17.4	2750	-	432	17.4
1987	550	17.4	2750	-	459	17.4
1734	700	17.3	3500	-	0	17.3
1578	850	19.0	4250	0.8	1092	17.3
1701	750	17.3	3750	-	1221	17.3
1886	600	17.1	3000	-	60	17.1
1925	600	17.1	3000	-	1230	17.1
1698	750	16.7	3750	-	1110	16.7
1699	750	16.7	3750	-	1147	16.7
1700	750	16.7	3750	-	1184	16.7
1988	550	16.7	2750	-	486	16.7
1989	550	16.7	2750	-	513	16.7
1431	1000	16.6	5000	-	1100	16.6
1276	1200	19.9	6000	1.7	900	16.6
1806	650	16.6	3250	-	32	16.6
2071	500	16.4	2500	-	75	16.4
2016	550	16.4	2750	-	1242	16.4
1964	600	16.2	3000	-	2400	16.2
1965	600	16.2	3000	-	2430	16.2
1966	600	16.2	3000	-	2460	16.2
1967	600	16.2	3000	-	2490	16.2
1968	600	16.2	3000	-	2520	16.2
1969	600	16.2	3000	-	2550	16.2
1805	650	16.1	3250	-	0	16.1
1732	750	20.3	3750	2.1	2368	16.0
2080	500	15.9	2500	-	300	15.9
2081	500	15.8	2500	-	325	15.8
2082	500	15.8	2500	-	350	15.8
2083	500	15.8	2500	-	375	15.8
2084	500	15.8	2500	-	400	15.8
2085	500	15.8	2500	-	425	15.8
2086	500	15.8	2500	-	450	15.8
2087	500	15.8	2500	-	475	15.8
2088	500	15.8	2500	-	500	15.8
1477	950	15.8	4750	-	1128	15.8
2089	500	15.7	2500	-	525	15.7
1766	700	15.6	3500	-	1120	15.6
1767	700	15.6	3500	-	1155	15.6
1768	700	15.6	3500	-	1190	15.6
1885	600	15.3	3000	-	30	15.3
2090	500	15.2	2500	-	550	15.2
2091	500	15.2	2500	-	575	15.2
1639	800	15.0	4000	-	1240	15.0
1527	900	15.0	4500	-	1170	15.0
1972	550	14.9	2750	-	54	14.9
2059	550	14.9	2750	-	2403	14.9
2060	550	14.9	2750	-	2430	14.9
2061	550	14.9	2750	-	2457	14.9
2062	550	14.9	2750	-	2484	14.9
2063	550	14.9	2750	-	2511	14.9
2064	550	14.9	2750	-	2538	14.9
2065	550	14.9	2750	-	2565	14.9
2066	550	14.9	2750	-	2592	14.9
2067	550	14.9	2750	-	2619	14.9
1884	600	14.9	3000	-	0	14.9
1848	650	14.8	3250	-	1376	14.8
1244	1250	20.8	6250	3.0	868	14.7
1772	700	14.6	3500	-	1330	14.6
1348	1100	18.3	5500	1.8	990	14.6
1840	650	14.5	3250	-	1120	14.5
1841	650	14.5	3250	-	1152	14.5
1842	650	14.5	3250	-	1184	14.5
1843	650	14.5	3250	-	1216	14.5
1581	850	14.1	4250	-	1218	14.1
1971	550	14.0	2750	-	27	14.0
1388	1050	17.4	5250	1.9	1040	13.6
1970	550	13.6	2750	-	0	13.6
2173	500	13.6	2500	-	2625	13.6
2164	500	13.5	2500	-	2400	13.5
2165	500	13.5	2500	-	2425	13.5
2166	500	13.5	2500	-	2450	13.5
2167	500	13.5	2500	-	2475	13.5
2168	500	13.5	2500	-	2500	13.5
2169	500	13.5	2500	-	2525	13.5
2170	500	13.5	2500	-	2550	13.5
2171	500	13.5	2500	-	2575	13.5
2172	500	13.5	2500	-	2600	13.5
1921	600	13.4	3000	-	1110	13.4
1922	600	13.4	3000	-	1140	13.4
1923	600	13.4	3000	-	1170	13.4
1924	600	13.4	3000	-	1200	13.4
1640	800	13.3	4000	-	1280	13.3
2070	500	13.2	2500	-	50	13.2
1703	750	13.0	3750	-	1295	13.0
1667	800	18.8	4000	3.0	2360	12.8
2069	500	12.8	2500	-	25	12.8
2117	500	12.7	2500	-	1225	12.7
1879	650	17.6	3250	2.5	2368	12.6
1704	750	12.5	3750	-	1332	12.5
2068	500	12.4	2500	-	0	12.4
1311	1150	19.1	5750	3.4	969	12.3
2011	550	12.3	2750	-	1107	12.3
2012	550	12.3	2750	-	1134	12.3
2013	550	12.3	2750	-	1161	12.3
2014	550	12.3	2750	-	1188	12.3
2015	550	12.3	2750	-	1215	12.3
2058	550	14.9	2750	1.5	2376	12.0
1582	850	14.1	4250	1.2	1260	11.8
2129	500	11.7	2500	-	1525	11.7
1773	700	11.6	3500	-	1365	11.6
1774	700	11.6	3500	-	1400	11.6
1528	900	15.0	4500	1.7	1215	11.6
1214	1300	21.6	6500	5.0	845	11.6
1963	600	16.2	3000	2.3	2370	11.5
1932	600	11.3	3000	-	1440	11.3
2112	500	11.2	2500	-	1100	11.2
2113	500	11.2	2500	-	1125	11.2
2114	500	11.2	2500	-	1150	11.2
2115	500	11.2	2500	-	1175	11.2
2116	500	11.2	2500	-	1200	11.2
1849	650	10.8	3250	-	1408	10.8
1850	650	10.8	3250	-	1440	10.8
2025	550	10.6	2750	-	1485	10.6
1478	950	15.8	4750	2.6	1175	10.5
2163	500	13.5	2500	1.8	2375	9.9
1641	800	13.3	4000	2.5	1320	8.3
1389	1050	17.4	5250	4.7	1092	8.1
1705	750	12.5	3750	2.5	1369	7.4
1432	1000	16.6	5000	5.0	1150	6.6
1851	650	10.8	3250	3.4	1472	4.0
1775	700	11.6	3500	5.0	1435	1.6

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
1262	1200	31.9	6000	1.7	60	28.6
1230	1250	30.9	6250	1.6	0	27.7
1295	1150	30.6	5750	1.7	57	27.1
1296	1150	30.6	5750	1.7	114	27.1
1261	1200	29.7	6000	1.7	0	26.4
1411	1000	30.3	5000	2.0	100	26.3
1331	1100	29.3	5500	1.8	55	25.6
1332	1100	29.3	5500	1.8	110	25.6
1333	1100	29.3	5500	1.8	165	25.6
1294	1150	28.5	5750	1.7	0	25.0
1235	1250	27.9	6250	1.6	310	24.7
1236	1250	27.9	6250	1.6	372	24.7
1237	1250	27.9	6250	1.6	434	24.7
1238	1250	27.9	6250	1.6	496	24.7
1239	1250	27.9	6250	1.6	558	24.7
1240	1250	27.9	6250	1.6	620	24.7
1241	1250	27.9	6250	1.6	682	24.7
1242	1250	27.9	6250	1.6	744	24.7
1370	1050	27.9	5250	1.9	104	24.1
1371	1050	27.9	5250	1.9	156	24.1
1372	1050	27.9	5250	1.9	208	24.1
1369	1050	27.6	5250	1.9	52	23.8
1330	1100	27.2	5500	1.8	0	23.6
1263	1200	26.8	6000	1.7	120	23.5
1264	1200	26.8	6000	1.7	180	23.5
1265	1200	26.8	6000	1.7	240	23.5
1266	1200	26.8	6000	1.7	300	23.5
1267	1200	26.8	6000	1.7	360	23.5
1268	1200	26.8	6000	1.7	420	23.5
1269	1200	26.8	6000	1.7	480	23.5
1270	1200	26.8	6000	1.7	540	23.5
1271	1200	26.8	6000	1.7	600	23.5
1272	1200	26.8	6000	1.7	660	23.5
1273	1200	26.8	6000	1.7	720	23.5
1274	1200	26.8	6000	1.7	780	23.5
1412	1000	26.6	5000	2.0	150	22.6
1413	1000	26.6	5000	2.0	200	22.6
1414	1000	26.6	5000	2.0	250	22.6
1297	1150	25.7	5750	1.7	171	22.2
1298	1150	25.7	5750	1.7	228	22.2
1299	1150	25.7	5750	1.7	285	22.2
1300	1150	25.7	5750	1.7	342	22.2
1301	1150	25.7	5750	1.7	399	22.2
1302	1150	25.7	5750	1.7	456	22.2
1303	1150	25.7	5750	1.7	513	22.2
1304	1150	25.7	5750	1.7	570	22.2
1305	1150	25.7	5750	1.7	627	22.2
1306	1150	25.7	5750	1.7	684	22.2
1307	1150	25.7	5750	1.7	741	22.2
1308	1150	25.7	5750	1.7	798	22.2
1309	1150	25.7	5750	1.7	855	22.2
1457	950	26.1	4750	2.1	188	21.9
1506	900	25.6	4500	2.2	225	21.1
1458	950	25.3	4750	2.1	235	21.1
1459	950	25.3	4750	2.1	282	21.1
1558	850	25.8	4250	2.4	252	21.1
1334	1100	24.6	5500	1.8	220	20.9
1335	1100	24.6	5500	1.8	275	20.9
1336	1100	24.6	5500	1.8	330	20.9
1337	1100	24.6	5500	1.8	385	20.9
1338	1100	24.6	5500	1.8	440	20.9
1339	1100	24.6	5500	1.8	495	20.9
1340	1100	24.6	5500	1.8	550	20.9
1341	1100	24.6	5500	1.8	605	20.9
1342	1100	24.6	5500	1.8	660	20.9
1343	1100	24.6	5500	1.8	715	20.9
1344	1100	24.6	5500	1.8	770	20.9
1345	1100	24.6	5500	1.8	825	20.9
1346	1100	24.6	5500	1.8	880	20.9
1373	1050	23.4	5250	1.9	260	19.6
1374	1050	23.4	5250	1.9	312	19.6
1375	1050	23.4	5250	1.9	364	19.6
1376	1050	23.4	5250	1.9	416	19.6
1377	1050	23.4	5250	1.9	468	19.6
1378	1050	23.4	5250	1.9	520	19.6
1379	1050	23.4	5250	1.9	572	19.6
1380	1050	23.4	5250	1.9	624	19.6
1381	1050	23.4	5250	1.9	676	19.6
1382	1050	23.4	5250	1.9	728	19.6
1383	1050	23.4	5250	1.9	780	19.6
1384	1050	23.4	5250	1.9	832	19.6
1385	1050	23.4	5250	1.9	884	19.6
1386	1050	23.4	5250	1.9	936	19.6
1507	900	23.9	4500	2.2	270	19.5
1508	900	23.9	4500	2.2	315	19.5
1509	900	23.9	4500	2.2	360	19.5
1275	1200	22.6	6000	1.7	840	19.2
1616	800	23.4	4000	2.5	320	18.4
1415	1000	22.3	5000	2.0	300	18.3
1416	1000	22.3	5000	2.0	350	18.3
1417	1000	22.3	5000	2.0	400	18.3
1418	1000	22.3	5000	2.0	450	18.3
1419	1000	22.3	5000	2.0	500	18.3
1420	1000	22.3	5000	2.0	550	18.3
1421	1000	22.3	5000	2.0	600	18.3
1422	1000	22.3	5000	2.0	650	18.3
1423	1000	22.3	5000	2.0	700	18.3
1424	1000	22.3	5000	2.0	750	18.3
1425	1000	22.3	5000	2.0	800	18.3
1426	1000	22.3	5000	2.0	850	18.3
1427	1000	22.3	5000	2.0	900	18.3
1428	1000	22.3	5000	2.0	950	18.3
1429	1000	22.3	5000	2.0	1000	18.3
1559	850	22.6	4250	2.4	294	17.9
1560	850	22.6	4250	2.4	336	17.9
1561	850	22.6	4250	2.4	378	17.9
1347	1100	21.2	5500	1.8	935	17.6
1460	950	21.8	4750	2.1	329	17.6
1243	1250	20.8	6250	1.6	806	17.6
1562	850	21.8	4250	2.4	420	17.1
1461	950	21.2	4750	2.1	376	17.0
1462	950	21.2	4750	2.1	423	17.0
1463	950	21.2	4750	2.1	470	17.0
1464	950	21.2	4750	2.1	517	17.0
1465	950	21.2	4750	2.1	564	17.0
1466	950	21.2	4750	2.1	611	17.0
1467	950	21.2	4750	2.1	658	17.0
1468	950	21.2	4750	2.1	705	17.0
1469	950	21.2	4750	2.1	752	17.0
1470	950	21.2	4750	2.1	799	17.0
1471	950	21.2	4750	2.1	846	17.0
1472	950	21.2	4750	2.1	893	17.0
1473	950	21.2	4750	2.1	940	17.0
1474	950	21.2	4750	2.1	987	17.0
1475	950	21.2	4750	2.1	1034	17.0
1678	750	21.9	3750	2.7	370	16.6
1617	800	21.3	4000	2.5	360	16.3
1618	800	21.3	4000	2.5	400	16.3
1619	800	21.3	4000	2.5	440	16.3
1387	1050	19.8	5250	1.9	988	16.0
1525	900	20.1	4500	2.1	1080	15.9
1476	950	19.5	4750	1.9	1081	15.7
1510	900	20.1	4500	2.2	405	15.6
1511	900	20.1	4500	2.2	450	15.6
1512	900	20.1	4500	2.2	495	15.6
1513	900	20.1	4500	2.2	540	15.6
1514	900	20.1	4500	2.2	585	15.6
1515	900	20.1	4500	2.2	630	15.6
1516	900	20.1	4500	2.2	675	15.6
1517	900	20.1	4500	2.2	720	15.6
1518	900	20.1	4500	2.2	765	15.6
1519	900	20.1	4500	2.2	810	15.6
1520	900	20.1	4500	2.2	855	15.6
1521	900	20.1	4500	2.2	900	15.6
1522	900	20.1	4500	2.2	945	15.6
1523	900	20.1	4500	2.2	990	15.6
1524	900	20.1	4500	2.2	1035	15.6
1310	1150	19.1	5750	1.7	912	15.6
1746	700	20.5	3500	2.9	420	14.8
1679	750	19.9	3750	2.7	407	14.6
1680	750	19.9	3750	2.7	444	14.6
1681	750	19.9	3750	2.7	481	14.6
1682	750	19.7	3750	2.7	518	14.4
1563	850	19.0	4250	2.4	462	14.3
1564	850	19.0	4250	2.4	504	14.3
1565	850	19.0	4250	2.4	546	14.3
1566	850	19.0	4250	2.4	588	14.3
1567	850	19.0	4250	2.4	630	14.3
1568	850	19.0	4250	2.4	672	14.3
1569	850	19.0	4250	2.4	714	14.3
1570	850	19.0	4250	2.4	756	14.3
1571	850	19.0	4250	2.4	798	14.3
1572	850	19.0	4250	2.4	840	14.3
1573	850	19.0	4250	2.4	882	14.3
1574	850	19.0	4250	2.4	924	14.3
1575	850	19.0	4250	2.4	966	14.3
1576	850	19.0	4250	2.4	1008	14.3
1577	850	19.0	4250	2.4	1050	14.3
1819	650	19.7	3250	2.9	448	13.9
1620	800	18.3	4000	2.5	480	13.3
1635	800	17.9	4000	2.4	1080	13.1
1747	700	18.6	3500	2.9	455	12.9
1748	700	18.6	3500	2.9	490	12.9
1749	700	18.6	3500	2.9	525	12.9
1750	700	18.6	3500	2.9	560	12.9
1621	800	17.9	4000	2.5	520	12.9
1622	800	17.9	4000	2.5	560	12.9
1623	800	17.9	4000	2.5	600	12.9
1624	800	17.9	4000	2.5	640	12.9
1625	800	17.9	4000	2.5	680	12.9
1626	800	17.9	4000	2.5	720	12.9
1627	800	17.9	4000	2.5	760	12.9
1628	800	17.9	4000	2.5	800	12.9
1629	800	17.9	4000	2.5	840	12.9
1630	800	17.9	4000	2.5	880	12.9
1631	800	17.9	4000	2.5	920	12.9
1632	800	17.9	4000	2.5	960	12.9
1633	800	17.9	4000	2.5	1000	12.9
1634	800	17.9	4000	2.5	1040	12.9
1430	1000	16.7	5000	2.0	1050	12.7
1990	550	16.7	2750	2.0	540	12.7
1765	700	15.6	3500	2.0	1085	11.6
1820	650	17.6	3250	3.1	480	11.4
1683	750	16.7	3750	2.7	555	11.4
1684	750	16.7	3750	2.7	592	11.4
1685	750	16.7	3750	2.7	629	11.4
1686	750	16.7	3750	2.7	666	11.4
1687	750	16.7	3750	2.7	703	11.4
1688	750	16.7	3750	2.7	740	11.4
1689	750	16.7	3750	2.7	777	11.4
1690	750	16.7	3750	2.7	814	11.4
1691	750	16.7	3750	2.7	851	11.4
1692	750	16.7	3750	2.7	888	11.4
1693	750	16.7	3750	2.7	925	11.4
1694	750	16.7	3750	2.7	962	11.4
1695	750	16.7	3750	2.7	999	11.4
1696	750	16.7	3750	2.7	1036	11.4
1697	750	16.7	3750	2.7	1073	11.4
1821	650	17.3	3250	3.1	512	11.1
1822	650	17.3	3250	3.1	544	11.1
1823	650	17.3	3250	3.1	576	11.1
1824	650	17.3	3250	3.1	608	11.1
1839	650	14.5	3250	1.7	1088	11.1
1901	600	17.6	3000	3.3	510	11.0
1751	700	15.6	3500	2.9	595	9.9
1752	700	15.6	3500	2.9	630	9.9
1753	700	15.6	3500	2.9	665	9.9
1754	700	15.6	3500	2.9	700	9.9
1755	700	15.6	3500	2.9	735	9.9
1756	700	15.6	3500	2.9	770	9.9
1757	700	15.6	3500	2.9	805	9.9
1758	700	15.6	3500	2.9	840	9.9
1759	700	15.6	3500	2.9	875	9.9
1760	700	15.6	3500	2.9	910	9.9
1761	700	15.6	3500	2.9	945	9.9
1762	700	15.6	3500	2.9	980	9.9
1763	700	15.6	3500	2.9	1015	9.9
1764	700	15.6	3500	2.9	1050	9.9
1902	600	16.5	3000	3.3	540	9.8
1903	600	16.0	3000	3.3	570	9.3
1904	600	16.0	3000	3.3	600	9.3
1905	600	16.0	3000	3.3	630	9.3
1906	600	16.0	3000	3.3	660	9.3
1991	550	16.1	2750	3.6	567	8.8
1825	650	14.5	3250	3.1	640	8.4
1826	650	14.5	3250	3.1	672	8.4
1827	650	14.5	3250	3.1	704	8.4
1828	650	14.5	3250	3.1	736	8.4
1829	650	14.5	3250	3.1	768	8.4
1830	650	14.5	3250	3.1	800	8.4
1831	650	14.5	3250	3.1	832	8.4
1832	650	14.5	3250	3.1	864	8.4
1833	650	14.5	3250	3.1	896	8.4
1834	650	14.5	3250	3.1	928	8.4
1835	650	14.5	3250	3.1	960	8.4
1836	650	14.5	3250	3.1	992	8.4
1837	650	14.5	3250	3.1	1024	8.4
1838	650	14.5	3250	3.1	1056	8.4
1992	550	14.6	2750	3.6	594	7.4
1993	550	14.6	2750	3.6	621	7.4
1994	550	14.6	2750	3.6	648	7.4
1995	550	14.6	2750	3.6	675	7.4
1996	550	14.6	2750	3.6	702	7.4
2092	500	15.2	2500	4.0	600	7.2
1920	600	13.4	3000	3.2	1080	7.1
1907	600	13.4	3000	3.3	690	6.7
1908	600	13.4	3000	3.3	720	6.7
1909	600	13.4	3000	3.3	750	6.7
1910	600	13.4	3000	3.3	780	6.7
1911	600	13.4	3000	3.3	810	6.7
1912	600	13.4	3000	3.3	840	6.7
1913	600	13.4	3000	3.3	870	6.7
1914	600	13.4	3000	3.3	900	6.7
1915	600	13.4	3000	3.3	930	6.7
1916	600	13.4	3000	3.3	960	6.7
1917	600	13.4	3000	3.3	990	6.7
1918	600	13.4	3000	3.3	1020	6.7
1919	600	13.4	3000	3.3	1050	6.7
2093	500	14.2	2500	4.0	625	6.2
2010	550	12.3	2750	3.5	1080	5.4
1997	550	12.6	2750	3.6	729	5.3
2094	500	13.3	2500	4.0	650	5.3
2095	500	13.3	2500	4.0	675	5.3
2096	500	13.3	2500	4.0	700	5.3
2097	500	13.3	2500	4.0	725	5.3
2098	500	13.3	2500	4.0	750	5.3
1998	550	12.3	2750	3.6	756	5.0
1999	550	12.3	2750	3.6	783	5.0
2000	550	12.3	2750	3.6	810	5.0
2001	550	12.3	2750	3.6	837	5.0
2002	550	12.3	2750	3.6	864	5.0
2003	550	12.3	2750	3.6	891	5.0
2004	550	12.3	2750	3.6	918	5.0
2005	550	12.3	2750	3.6	945	5.0
2006	550	12.3	2750	3.6	972	5.0
2007	550	12.3	2750	3.6	999	5.0
2008	550	12.3	2750	3.6	1026	5.0
2009	550	12.3	2750	3.6	1053	5.0
2099	500	12.4	2500	4.0	775	4.4
2100	500	11.2	2500	4.0	800	3.2
2101	500	11.2	2500	4.0	825	3.2
2102	500	11.2	2500	4.0	850	3.2
2103	500	11.2	2500	4.0	875	3.2
2104	500	11.2	2500	4.0	900	3.2
2105	500	11.2	2500	4.0	925	3.2
2106	500	11.2	2500	4.0	950	3.2
2107	500	11.2	2500	4.0	975	3.2
2108	500	11.2	2500	4.0	1000	3.2
2109	500	11.2	2500	4.0	1025	3.2
2110	500	11.2	2500	4.0	1050	3.2
2111	500	11.2	2500	4.0	1075	3.2

Overall score = score_ratio - 2 * repetitive DNA content