Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign48 - back to list

Summary
Image
Unique putative probes (0)
Putative probes (536)
Redundant probes (286)

Summary

`./analyse_probe_search_results : Wed Sep 21 13:57:40 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign48
  assembly      : NCBIM34
  bias          : 5prime
  chromosome    : 19
  id            : 30
  strand        : 1
  -------------

Design window length: 4001bp

All jobs for probe design are successful - total: 66
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 1318 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                : 0
Putative probes with single_self_hit        : 1318/1318
Putative probes exceeding selection criteria: 822/1318

PASSED (GREEN) AND FAILED PUTATIVE PROBES IN THE DESIGN WINDOW
--------------------------------------------------------------

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

No probes

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
32203	1300	40.1	6500	-	975	40.1
32204	1300	40.1	6500	-	1040	40.1
32205	1300	40.1	6500	-	1105	40.1
32206	1300	40.1	6500	-	1170	40.1
32207	1300	40.1	6500	-	1235	40.1
32228	1300	40.9	6500	0.8	2600	39.3
32272	1250	39.3	6250	-	2604	39.3
32246	1250	38.6	6250	-	992	38.6
32247	1250	38.6	6250	-	1054	38.6
32248	1250	38.6	6250	-	1116	38.6
32249	1250	38.6	6250	-	1178	38.6
32250	1250	38.6	6250	-	1240	38.6
32251	1250	38.6	6250	-	1302	38.6
32318	1200	37.7	6000	-	2580	37.7
32319	1200	37.7	6000	-	2640	37.7
32292	1200	37.0	6000	-	1020	37.0
32293	1200	37.0	6000	-	1080	37.0
32294	1200	37.0	6000	-	1140	37.0
32295	1200	37.0	6000	-	1200	37.0
32296	1200	37.0	6000	-	1260	37.0
32297	1200	37.0	6000	-	1320	37.0
32368	1150	36.2	5750	-	2622	36.2
32369	1150	36.2	5750	-	2679	36.2
32370	1150	36.2	5750	-	2736	36.2
32340	1150	35.5	5750	-	1026	35.5
32341	1150	35.5	5750	-	1083	35.5
32342	1150	35.5	5750	-	1140	35.5
32343	1150	35.5	5750	-	1197	35.5
32344	1150	35.5	5750	-	1254	35.5
32345	1150	35.5	5750	-	1311	35.5
32346	1150	35.5	5750	-	1368	35.5
32273	1250	39.3	6250	2.1	2666	35.1
32420	1100	34.6	5500	-	2585	34.6
32421	1100	34.6	5500	-	2640	34.6
32422	1100	34.6	5500	-	2695	34.6
32423	1100	34.6	5500	-	2750	34.6
32391	1100	34.0	5500	-	990	34.0
32392	1100	34.0	5500	-	1045	34.0
32393	1100	34.0	5500	-	1100	34.0
32394	1100	34.0	5500	-	1155	34.0
32395	1100	34.0	5500	-	1210	34.0
32396	1100	34.0	5500	-	1265	34.0
32397	1100	34.0	5500	-	1320	34.0
32398	1100	34.0	5500	-	1375	34.0
32399	1100	34.0	5500	-	1430	34.0
32367	1150	36.2	5750	1.3	2565	33.6
32476	1050	33.0	5250	-	2600	33.0
32477	1050	33.0	5250	-	2652	33.0
32478	1050	33.0	5250	-	2704	33.0
32479	1050	33.0	5250	-	2756	33.0
32480	1050	33.0	5250	-	2808	33.0
32445	1050	32.4	5250	-	988	32.4
32446	1050	32.4	5250	-	1040	32.4
32447	1050	32.4	5250	-	1092	32.4
32448	1050	32.4	5250	-	1144	32.4
32449	1050	32.4	5250	-	1196	32.4
32450	1050	32.4	5250	-	1248	32.4
32451	1050	32.4	5250	-	1300	32.4
32452	1050	32.4	5250	-	1352	32.4
32453	1050	32.4	5250	-	1404	32.4
32454	1050	32.4	5250	-	1456	32.4
32424	1100	34.6	5500	1.4	2805	31.9
32339	1150	31.6	5750	-	969	31.6
32535	1000	31.4	5000	-	2600	31.4
32536	1000	31.4	5000	-	2650	31.4
32537	1000	31.4	5000	-	2700	31.4
32538	1000	31.4	5000	-	2750	31.4
32539	1000	31.4	5000	-	2800	31.4
32540	1000	31.4	5000	-	2850	31.4
32455	1050	32.4	5250	0.6	1508	31.3
32503	1000	30.9	5000	-	1000	30.9
32504	1000	30.9	5000	-	1050	30.9
32505	1000	30.9	5000	-	1100	30.9
32506	1000	30.9	5000	-	1150	30.9
32507	1000	30.9	5000	-	1200	30.9
32508	1000	30.9	5000	-	1250	30.9
32509	1000	30.9	5000	-	1300	30.9
32510	1000	30.9	5000	-	1350	30.9
32511	1000	30.9	5000	-	1400	30.9
32512	1000	30.9	5000	-	1450	30.9
32513	1000	30.9	5000	-	1500	30.9
32514	1000	30.9	5000	-	1550	30.9
32599	950	29.9	4750	-	2585	29.9
32600	950	29.9	4750	-	2632	29.9
32601	950	29.9	4750	-	2679	29.9
32602	950	29.9	4750	-	2726	29.9
32603	950	29.9	4750	-	2773	29.9
32604	950	29.9	4750	-	2820	29.9
32605	950	29.9	4750	-	2867	29.9
32606	950	29.9	4750	-	2914	29.9
32565	950	29.3	4750	-	987	29.3
32566	950	29.3	4750	-	1034	29.3
32567	950	29.3	4750	-	1081	29.3
32568	950	29.3	4750	-	1128	29.3
32569	950	29.3	4750	-	1175	29.3
32570	950	29.3	4750	-	1222	29.3
32571	950	29.3	4750	-	1269	29.3
32572	950	29.3	4750	-	1316	29.3
32573	950	29.3	4750	-	1363	29.3
32574	950	29.3	4750	-	1410	29.3
32575	950	29.3	4750	-	1457	29.3
32576	950	29.3	4750	-	1504	29.3
32577	950	29.3	4750	-	1551	29.3
32578	950	29.3	4750	-	1598	29.3
32481	1050	33.0	5250	1.9	2860	29.2
32291	1200	28.8	6000	-	960	28.8
32667	900	28.3	4500	-	2610	28.3
32668	900	28.3	4500	-	2655	28.3
32669	900	28.3	4500	-	2700	28.3
32670	900	28.3	4500	-	2745	28.3
32671	900	28.3	4500	-	2790	28.3
32672	900	28.3	4500	-	2835	28.3
32673	900	28.3	4500	-	2880	28.3
32674	900	28.3	4500	-	2925	28.3
32675	900	28.3	4500	-	2970	28.3
32631	900	27.8	4500	-	990	27.8
32632	900	27.8	4500	-	1035	27.8
32633	900	27.8	4500	-	1080	27.8
32634	900	27.8	4500	-	1125	27.8
32635	900	27.8	4500	-	1170	27.8
32636	900	27.8	4500	-	1215	27.8
32637	900	27.8	4500	-	1260	27.8
32638	900	27.8	4500	-	1305	27.8
32639	900	27.8	4500	-	1350	27.8
32640	900	27.8	4500	-	1395	27.8
32641	900	27.8	4500	-	1440	27.8
32642	900	27.8	4500	-	1485	27.8
32643	900	27.8	4500	-	1530	27.8
32644	900	27.8	4500	-	1575	27.8
32645	900	27.8	4500	-	1620	27.8
32347	1150	31.8	5750	2.0	1425	27.8
32702	850	26.9	4250	-	1008	26.9
32741	850	26.7	4250	-	2646	26.7
32742	850	26.7	4250	-	2688	26.7
32743	850	26.7	4250	-	2730	26.7
32744	850	26.7	4250	-	2772	26.7
32745	850	26.7	4250	-	2814	26.7
32746	850	26.7	4250	-	2856	26.7
32747	850	26.7	4250	-	2898	26.7
32748	850	26.7	4250	-	2940	26.7
32749	850	26.7	4250	-	2982	26.7
32750	850	26.7	4250	-	3024	26.7
32779	800	26.7	4000	-	1000	26.7
32780	800	26.7	4000	-	1040	26.7
32703	850	26.2	4250	-	1050	26.2
32704	850	26.2	4250	-	1092	26.2
32705	850	26.2	4250	-	1134	26.2
32706	850	26.2	4250	-	1176	26.2
32707	850	26.2	4250	-	1218	26.2
32708	850	26.2	4250	-	1260	26.2
32709	850	26.2	4250	-	1302	26.2
32710	850	26.2	4250	-	1344	26.2
32711	850	26.2	4250	-	1386	26.2
32712	850	26.2	4250	-	1428	26.2
32713	850	26.2	4250	-	1470	26.2
32714	850	26.2	4250	-	1512	26.2
32715	850	26.2	4250	-	1554	26.2
32716	850	26.2	4250	-	1596	26.2
32717	850	26.2	4250	-	1638	26.2
32718	850	26.2	4250	-	1680	26.2
32208	1300	29.8	6500	2.2	1300	25.5
32227	1300	29.5	6500	2.2	2535	25.2
32821	800	25.2	4000	-	2680	25.2
32822	800	25.2	4000	-	2720	25.2
32823	800	25.2	4000	-	2760	25.2
32824	800	25.2	4000	-	2800	25.2
32825	800	25.2	4000	-	2840	25.2
32826	800	25.2	4000	-	2880	25.2
32827	800	25.2	4000	-	2920	25.2
32828	800	25.2	4000	-	2960	25.2
32829	800	25.2	4000	-	3000	25.2
32830	800	25.2	4000	-	3040	25.2
32831	800	25.2	4000	-	3080	25.2
32666	900	28.5	4500	1.7	2565	25.1
32862	750	25.0	3750	-	999	25.0
32863	750	25.0	3750	-	1036	25.0
32864	750	25.0	3750	-	1073	25.0
32646	900	27.8	4500	1.4	1665	24.9
32781	800	24.7	4000	-	1080	24.7
32782	800	24.7	4000	-	1120	24.7
32783	800	24.7	4000	-	1160	24.7
32784	800	24.7	4000	-	1200	24.7
32785	800	24.7	4000	-	1240	24.7
32786	800	24.7	4000	-	1280	24.7
32787	800	24.7	4000	-	1320	24.7
32788	800	24.7	4000	-	1360	24.7
32789	800	24.7	4000	-	1400	24.7
32790	800	24.7	4000	-	1440	24.7
32791	800	24.7	4000	-	1480	24.7
32792	800	24.7	4000	-	1520	24.7
32793	800	24.7	4000	-	1560	24.7
32794	800	24.7	4000	-	1600	24.7
32795	800	24.7	4000	-	1640	24.7
32796	800	24.7	4000	-	1680	24.7
32797	800	24.7	4000	-	1720	24.7
32209	1300	28.9	6500	2.2	1365	24.6
32210	1300	28.9	6500	2.2	1430	24.6
32211	1300	28.9	6500	2.2	1495	24.6
32212	1300	28.9	6500	2.2	1560	24.6
32213	1300	28.9	6500	2.2	1625	24.6
32214	1300	28.9	6500	2.2	1690	24.6
32215	1300	28.9	6500	2.2	1755	24.6
32216	1300	28.9	6500	2.2	1820	24.6
32217	1300	28.9	6500	2.2	1885	24.6
32218	1300	28.9	6500	2.2	1950	24.6
32219	1300	28.9	6500	2.2	2015	24.6
32220	1300	28.9	6500	2.2	2080	24.6
32221	1300	28.9	6500	2.2	2145	24.6
32222	1300	28.9	6500	2.2	2210	24.6
32223	1300	28.9	6500	2.2	2275	24.6
32224	1300	28.9	6500	2.2	2340	24.6
32225	1300	28.9	6500	2.2	2405	24.6
32226	1300	28.9	6500	2.2	2470	24.6
32909	750	23.6	3750	-	2738	23.6
32910	750	23.6	3750	-	2775	23.6
32911	750	23.6	3750	-	2812	23.6
32912	750	23.6	3750	-	2849	23.6
32913	750	23.6	3750	-	2886	23.6
32914	750	23.6	3750	-	2923	23.6
32915	750	23.6	3750	-	2960	23.6
32916	750	23.6	3750	-	2997	23.6
32917	750	23.6	3750	-	3034	23.6
32918	750	23.6	3750	-	3071	23.6
32919	750	23.6	3750	-	3108	23.6
32951	700	23.3	3500	-	980	23.3
32952	700	23.3	3500	-	1015	23.3
32953	700	23.3	3500	-	1050	23.3
32954	700	23.3	3500	-	1085	23.3
32955	700	23.3	3500	-	1120	23.3
32252	1250	27.8	6250	2.2	1364	23.3
32253	1250	27.8	6250	2.2	1426	23.3
32254	1250	27.8	6250	2.2	1488	23.3
32255	1250	27.8	6250	2.2	1550	23.3
32256	1250	27.8	6250	2.2	1612	23.3
32865	750	23.1	3750	-	1110	23.1
32866	750	23.1	3750	-	1147	23.1
32867	750	23.1	3750	-	1184	23.1
32868	750	23.1	3750	-	1221	23.1
32869	750	23.1	3750	-	1258	23.1
32870	750	23.1	3750	-	1295	23.1
32871	750	23.1	3750	-	1332	23.1
32872	750	23.1	3750	-	1369	23.1
32873	750	23.1	3750	-	1406	23.1
32874	750	23.1	3750	-	1443	23.1
32875	750	23.1	3750	-	1480	23.1
32876	750	23.1	3750	-	1517	23.1
32877	750	23.1	3750	-	1554	23.1
32878	750	23.1	3750	-	1591	23.1
32879	750	23.1	3750	-	1628	23.1
32880	750	23.1	3750	-	1665	23.1
32881	750	23.1	3750	-	1702	23.1
32882	750	23.1	3750	-	1739	23.1
32883	750	23.1	3750	-	1776	23.1
32502	1000	23.0	5000	-	950	23.0
32798	800	24.7	4000	1.0	1760	22.7
32371	1150	31.8	5750	4.6	2793	22.6
32245	1250	22.5	6250	-	930	22.5
32920	750	23.6	3750	0.7	3145	22.3
32956	700	22.2	3500	-	1155	22.2
33003	700	22.0	3500	-	2800	22.0
33004	700	22.0	3500	-	2835	22.0
33005	700	22.0	3500	-	2870	22.0
33006	700	22.0	3500	-	2905	22.0
33007	700	22.0	3500	-	2940	22.0
33008	700	22.0	3500	-	2975	22.0
33009	700	22.0	3500	-	3010	22.0
33010	700	22.0	3500	-	3045	22.0
33011	700	22.0	3500	-	3080	22.0
33012	700	22.0	3500	-	3115	22.0
33013	700	22.0	3500	-	3150	22.0
33014	700	22.0	3500	-	3185	22.0
33049	650	21.7	3250	-	992	21.7
33050	650	21.7	3250	-	1024	21.7
33051	650	21.7	3250	-	1056	21.7
33052	650	21.7	3250	-	1088	21.7
33053	650	21.7	3250	-	1120	21.7
33054	650	21.7	3250	-	1152	21.7
33055	650	21.7	3250	-	1184	21.7
32957	700	21.6	3500	-	1190	21.6
32958	700	21.6	3500	-	1225	21.6
32959	700	21.6	3500	-	1260	21.6
32960	700	21.6	3500	-	1295	21.6
32961	700	21.6	3500	-	1330	21.6
32962	700	21.6	3500	-	1365	21.6
32963	700	21.6	3500	-	1400	21.6
32964	700	21.6	3500	-	1435	21.6
32965	700	21.6	3500	-	1470	21.6
32966	700	21.6	3500	-	1505	21.6
32967	700	21.6	3500	-	1540	21.6
32968	700	21.6	3500	-	1575	21.6
32969	700	21.6	3500	-	1610	21.6
32970	700	21.6	3500	-	1645	21.6
32971	700	21.6	3500	-	1680	21.6
32972	700	21.6	3500	-	1715	21.6
32973	700	21.6	3500	-	1750	21.6
32974	700	21.6	3500	-	1785	21.6
32701	850	21.6	4250	-	966	21.6
32739	850	25.0	4250	2.1	2562	20.8
33107	650	20.4	3250	-	2848	20.4
33108	650	20.4	3250	-	2880	20.4
33109	650	20.4	3250	-	2912	20.4
33110	650	20.4	3250	-	2944	20.4
33111	650	20.4	3250	-	2976	20.4
33112	650	20.4	3250	-	3008	20.4
33113	650	20.4	3250	-	3040	20.4
33114	650	20.4	3250	-	3072	20.4
33115	650	20.4	3250	-	3104	20.4
33116	650	20.4	3250	-	3136	20.4
33117	650	20.4	3250	-	3168	20.4
33118	650	20.4	3250	-	3200	20.4
33119	650	20.4	3250	-	3232	20.4
32390	1100	20.3	5500	-	935	20.3
33056	650	20.1	3250	-	1216	20.1
33057	650	20.1	3250	-	1248	20.1
33058	650	20.1	3250	-	1280	20.1
33059	650	20.1	3250	-	1312	20.1
33060	650	20.1	3250	-	1344	20.1
33061	650	20.1	3250	-	1376	20.1
33062	650	20.1	3250	-	1408	20.1
33063	650	20.1	3250	-	1440	20.1
33064	650	20.1	3250	-	1472	20.1
33065	650	20.1	3250	-	1504	20.1
33066	650	20.1	3250	-	1536	20.1
33067	650	20.1	3250	-	1568	20.1
33068	650	20.1	3250	-	1600	20.1
33069	650	20.1	3250	-	1632	20.1
33070	650	20.1	3250	-	1664	20.1
33071	650	20.1	3250	-	1696	20.1
33072	650	20.1	3250	-	1728	20.1
33073	650	20.1	3250	-	1760	20.1
33074	650	20.1	3250	-	1792	20.1
33158	600	20.0	3000	-	1050	20.0
33159	600	20.0	3000	-	1080	20.0
33160	600	20.0	3000	-	1110	20.0
33161	600	20.0	3000	-	1140	20.0
33162	600	20.0	3000	-	1170	20.0
33163	600	20.0	3000	-	1200	20.0
33164	600	20.0	3000	-	1230	20.0
32444	1050	19.7	5250	-	936	19.7
32630	900	19.6	4500	-	945	19.6
32202	1300	19.5	6500	-	910	19.5
32778	800	19.2	4000	-	960	19.2
33183	600	19.2	3000	-	1800	19.2
33219	600	18.9	3000	-	2880	18.9
33220	600	18.9	3000	-	2910	18.9
33221	600	18.9	3000	-	2940	18.9
33222	600	18.9	3000	-	2970	18.9
33223	600	18.9	3000	-	3000	18.9
33224	600	18.9	3000	-	3030	18.9
33225	600	18.9	3000	-	3060	18.9
33226	600	18.9	3000	-	3090	18.9
33227	600	18.9	3000	-	3120	18.9
33228	600	18.9	3000	-	3150	18.9
33229	600	18.9	3000	-	3180	18.9
33230	600	18.9	3000	-	3210	18.9
33231	600	18.9	3000	-	3240	18.9
33232	600	18.9	3000	-	3270	18.9
32564	950	18.6	4750	-	940	18.6
33165	600	18.5	3000	-	1260	18.5
33166	600	18.5	3000	-	1290	18.5
33167	600	18.5	3000	-	1320	18.5
33168	600	18.5	3000	-	1350	18.5
33169	600	18.5	3000	-	1380	18.5
33170	600	18.5	3000	-	1410	18.5
33171	600	18.5	3000	-	1440	18.5
33172	600	18.5	3000	-	1470	18.5
33173	600	18.5	3000	-	1500	18.5
33174	600	18.5	3000	-	1530	18.5
33175	600	18.5	3000	-	1560	18.5
33176	600	18.5	3000	-	1590	18.5
33177	600	18.5	3000	-	1620	18.5
33178	600	18.5	3000	-	1650	18.5
33179	600	18.5	3000	-	1680	18.5
33180	600	18.5	3000	-	1710	18.5
33181	600	18.5	3000	-	1740	18.5
33182	600	18.5	3000	-	1770	18.5
33278	550	18.3	2750	-	1107	18.3
33279	550	18.3	2750	-	1134	18.3
33280	550	18.3	2750	-	1161	18.3
33281	550	18.3	2750	-	1188	18.3
33282	550	18.3	2750	-	1215	18.3
33283	550	18.3	2750	-	1242	18.3
33284	550	18.3	2750	-	1269	18.3
32861	750	18.0	3750	-	962	18.0
32290	1200	18.0	6000	-	900	18.0
32832	800	25.2	4000	3.8	3120	17.7
33285	550	17.4	2750	-	1296	17.4
33346	550	17.3	2750	-	2943	17.3
33347	550	17.3	2750	-	2970	17.3
33348	550	17.3	2750	-	2997	17.3
33349	550	17.3	2750	-	3024	17.3
33350	550	17.3	2750	-	3051	17.3
33351	550	17.3	2750	-	3078	17.3
33352	550	17.3	2750	-	3105	17.3
33353	550	17.3	2750	-	3132	17.3
33354	550	17.3	2750	-	3159	17.3
33355	550	17.3	2750	-	3186	17.3
33356	550	17.3	2750	-	3213	17.3
33357	550	17.3	2750	-	3240	17.3
33358	550	17.3	2750	-	3267	17.3
33359	550	17.3	2750	-	3294	17.3
33360	550	17.3	2750	-	3321	17.3
32338	1150	17.2	5750	-	912	17.2
33286	550	17.0	2750	-	1323	17.0
33287	550	17.0	2750	-	1350	17.0
33288	550	17.0	2750	-	1377	17.0
33289	550	17.0	2750	-	1404	17.0
33290	550	17.0	2750	-	1431	17.0
33291	550	17.0	2750	-	1458	17.0
33292	550	17.0	2750	-	1485	17.0
33293	550	17.0	2750	-	1512	17.0
33294	550	17.0	2750	-	1539	17.0
33295	550	17.0	2750	-	1566	17.0
33296	550	17.0	2750	-	1593	17.0
33297	550	17.0	2750	-	1620	17.0
33298	550	17.0	2750	-	1647	17.0
33299	550	17.0	2750	-	1674	17.0
33300	550	17.0	2750	-	1701	17.0
33301	550	17.0	2750	-	1728	17.0
33302	550	17.0	2750	-	1755	17.0
33303	550	17.0	2750	-	1782	17.0
33411	500	16.7	2500	-	1150	16.7
33412	500	16.7	2500	-	1175	16.7
33413	500	16.7	2500	-	1200	16.7
33414	500	16.7	2500	-	1225	16.7
33415	500	16.7	2500	-	1250	16.7
33416	500	16.7	2500	-	1275	16.7
33417	500	16.7	2500	-	1300	16.7
33418	500	16.7	2500	-	1325	16.7
33273	550	16.5	2750	-	972	16.5
33437	500	16.0	2500	-	1800	16.0
33404	500	15.9	2500	-	975	15.9
33419	500	15.8	2500	-	1350	15.8
33485	500	15.7	2500	-	3000	15.7
33486	500	15.7	2500	-	3025	15.7
33487	500	15.7	2500	-	3050	15.7
33488	500	15.7	2500	-	3075	15.7
33489	500	15.7	2500	-	3100	15.7
33490	500	15.7	2500	-	3125	15.7
33491	500	15.7	2500	-	3150	15.7
33492	500	15.7	2500	-	3175	15.7
33493	500	15.7	2500	-	3200	15.7
33494	500	15.7	2500	-	3225	15.7
33495	500	15.7	2500	-	3250	15.7
33496	500	15.7	2500	-	3275	15.7
33497	500	15.7	2500	-	3300	15.7
33498	500	15.7	2500	-	3325	15.7
33499	500	15.7	2500	-	3350	15.7
33500	500	15.7	2500	-	3375	15.7
33048	650	15.6	3250	-	960	15.6
33420	500	15.4	2500	-	1375	15.4
33421	500	15.4	2500	-	1400	15.4
33422	500	15.4	2500	-	1425	15.4
33423	500	15.4	2500	-	1450	15.4
33424	500	15.4	2500	-	1475	15.4
33425	500	15.4	2500	-	1500	15.4
33426	500	15.4	2500	-	1525	15.4
33427	500	15.4	2500	-	1550	15.4
33428	500	15.4	2500	-	1575	15.4
33429	500	15.4	2500	-	1600	15.4
33430	500	15.4	2500	-	1625	15.4
33431	500	15.4	2500	-	1650	15.4
33432	500	15.4	2500	-	1675	15.4
33433	500	15.4	2500	-	1700	15.4
33434	500	15.4	2500	-	1725	15.4
33435	500	15.4	2500	-	1750	15.4
33436	500	15.4	2500	-	1775	15.4
32244	1250	15.3	6250	-	868	15.3
32198	1300	15.2	6500	-	650	15.2
32199	1300	15.2	6500	-	715	15.2
32200	1300	15.2	6500	-	780	15.2
32201	1300	15.2	6500	-	845	15.2
32950	700	15.2	3500	-	945	15.2
32501	1000	15.0	5000	-	900	15.0
32389	1100	14.9	5500	-	880	14.9
32240	1250	14.6	6250	-	620	14.6
32241	1250	14.6	6250	-	682	14.6
32242	1250	14.6	6250	-	744	14.6
32243	1250	14.6	6250	-	806	14.6
32443	1050	14.4	5250	-	884	14.4
33155	600	14.4	3000	-	960	14.4
32285	1200	14.1	6000	-	600	14.1
32286	1200	14.1	6000	-	660	14.1
32287	1200	14.1	6000	-	720	14.1
32288	1200	14.1	6000	-	780	14.1
32289	1200	14.1	6000	-	840	14.1
32700	850	13.8	4250	-	924	13.8
32563	950	13.6	4750	-	893	13.6
32337	1150	13.5	5750	-	855	13.5
32629	900	13.5	4500	-	900	13.5
32333	1150	13.5	5750	-	627	13.5
32334	1150	13.5	5750	-	684	13.5
32335	1150	13.5	5750	-	741	13.5
32336	1150	13.5	5750	-	798	13.5
33015	700	22.0	3500	4.3	3220	13.4
32197	1300	13.0	6500	-	585	13.0
32384	1100	12.9	5500	-	605	12.9
32385	1100	12.9	5500	-	660	12.9
32386	1100	12.9	5500	-	715	12.9
32387	1100	12.9	5500	-	770	12.9
32388	1100	12.9	5500	-	825	12.9
32777	800	12.5	4000	-	920	12.5
32860	750	12.4	3750	-	925	12.4
32438	1050	12.3	5250	-	624	12.3
32439	1050	12.3	5250	-	676	12.3
32440	1050	12.3	5250	-	728	12.3
32441	1050	12.3	5250	-	780	12.3
32442	1050	12.3	5250	-	832	12.3
33272	550	12.0	2750	-	945	12.0
32495	1000	11.7	5000	-	600	11.7
32496	1000	11.7	5000	-	650	11.7
32497	1000	11.7	5000	-	700	11.7
32498	1000	11.7	5000	-	750	11.7
32499	1000	11.7	5000	-	800	11.7
32500	1000	11.7	5000	-	850	11.7
33403	500	11.5	2500	-	950	11.5
32699	850	11.5	4250	-	882	11.5
33047	650	11.3	3250	-	928	11.3
32557	950	11.1	4750	-	611	11.1
32558	950	11.1	4750	-	658	11.1
32559	950	11.1	4750	-	705	11.1
32560	950	11.1	4750	-	752	11.1
32561	950	11.1	4750	-	799	11.1
32562	950	11.1	4750	-	846	11.1
32776	800	10.8	4000	-	880	10.8
33154	600	10.8	3000	-	930	10.8
32859	750	10.6	3750	-	888	10.6
32628	900	10.6	4500	-	855	10.6
32623	900	10.5	4500	-	630	10.5
32624	900	10.5	4500	-	675	10.5
32625	900	10.5	4500	-	720	10.5
32626	900	10.5	4500	-	765	10.5
32627	900	10.5	4500	-	810	10.5
32949	700	10.5	3500	-	910	10.5
32239	1250	10.3	6250	-	558	10.3
32332	1150	10.2	5750	-	570	10.2

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
32320	1200	37.7	6000	0.8	2700	36.1
32541	1000	31.4	5000	1.0	2900	29.4
32607	950	29.9	4750	2.2	2961	25.5
32298	1200	29.1	6000	2.3	1380	24.5
32271	1250	28.5	6250	2.2	2542	24.1
32257	1250	27.8	6250	2.2	1674	23.3
32258	1250	27.8	6250	2.2	1736	23.3
32259	1250	27.8	6250	2.2	1798	23.3
32260	1250	27.8	6250	2.2	1860	23.3
32261	1250	27.8	6250	2.2	1922	23.3
32262	1250	27.8	6250	2.2	1984	23.3
32263	1250	27.8	6250	2.2	2046	23.3
32264	1250	27.8	6250	2.2	2108	23.3
32265	1250	27.8	6250	2.2	2170	23.3
32266	1250	27.8	6250	2.2	2232	23.3
32267	1250	27.8	6250	2.2	2294	23.3
32268	1250	27.8	6250	2.2	2356	23.3
32269	1250	27.8	6250	2.2	2418	23.3
32270	1250	27.8	6250	2.2	2480	23.3
32676	900	28.3	4500	2.8	3015	22.7
32317	1200	27.3	6000	2.3	2520	22.6
32299	1200	26.7	6000	2.3	1440	22.0
32300	1200	26.7	6000	2.3	1500	22.0
32301	1200	26.7	6000	2.3	1560	22.0
32302	1200	26.7	6000	2.3	1620	22.0
32303	1200	26.7	6000	2.3	1680	22.0
32304	1200	26.7	6000	2.3	1740	22.0
32305	1200	26.7	6000	2.3	1800	22.0
32306	1200	26.7	6000	2.3	1860	22.0
32307	1200	26.7	6000	2.3	1920	22.0
32308	1200	26.7	6000	2.3	1980	22.0
32309	1200	26.7	6000	2.3	2040	22.0
32310	1200	26.7	6000	2.3	2100	22.0
32311	1200	26.7	6000	2.3	2160	22.0
32312	1200	26.7	6000	2.3	2220	22.0
32313	1200	26.7	6000	2.3	2280	22.0
32314	1200	26.7	6000	2.3	2340	22.0
32315	1200	26.7	6000	2.3	2400	22.0
32316	1200	26.7	6000	2.3	2460	22.0
32400	1100	26.7	5500	2.5	1485	21.6
32366	1150	26.0	5750	2.4	2508	21.1
32348	1150	25.6	5750	2.4	1482	20.7
32349	1150	25.6	5750	2.4	1539	20.7
32350	1150	25.6	5750	2.4	1596	20.7
32351	1150	25.6	5750	2.4	1653	20.7
32352	1150	25.6	5750	2.4	1710	20.7
32353	1150	25.6	5750	2.4	1767	20.7
32354	1150	25.6	5750	2.4	1824	20.7
32355	1150	25.6	5750	2.4	1881	20.7
32356	1150	25.6	5750	2.4	1938	20.7
32357	1150	25.6	5750	2.4	1995	20.7
32358	1150	25.6	5750	2.4	2052	20.7
32359	1150	25.6	5750	2.4	2109	20.7
32360	1150	25.6	5750	2.4	2166	20.7
32361	1150	25.6	5750	2.4	2223	20.7
32362	1150	25.6	5750	2.4	2280	20.7
32363	1150	25.6	5750	2.4	2337	20.7
32364	1150	25.6	5750	2.4	2394	20.7
32365	1150	25.6	5750	2.4	2451	20.7
32751	850	26.7	4250	3.1	3066	20.6
32719	850	25.1	4250	2.4	1722	20.4
32419	1100	25.0	5500	2.5	2530	19.9
32401	1100	24.4	5500	2.5	1540	19.4
32402	1100	24.4	5500	2.5	1595	19.4
32403	1100	24.4	5500	2.5	1650	19.4
32404	1100	24.4	5500	2.5	1705	19.4
32405	1100	24.4	5500	2.5	1760	19.4
32406	1100	24.4	5500	2.5	1815	19.4
32407	1100	24.4	5500	2.5	1870	19.4
32408	1100	24.4	5500	2.5	1925	19.4
32409	1100	24.4	5500	2.5	1980	19.4
32410	1100	24.4	5500	2.5	2035	19.4
32411	1100	24.4	5500	2.5	2090	19.4
32412	1100	24.4	5500	2.5	2145	19.4
32413	1100	24.4	5500	2.5	2200	19.4
32414	1100	24.4	5500	2.5	2255	19.4
32415	1100	24.4	5500	2.5	2310	19.4
32416	1100	24.4	5500	2.5	2365	19.4
32417	1100	24.4	5500	2.5	2420	19.4
32418	1100	24.4	5500	2.5	2475	19.4
32475	1050	24.2	5250	2.7	2548	18.9
32456	1050	23.3	5250	2.7	1560	18.0
32457	1050	23.3	5250	2.7	1612	18.0
32458	1050	23.3	5250	2.7	1664	18.0
32459	1050	23.3	5250	2.7	1716	18.0
32460	1050	23.3	5250	2.7	1768	18.0
32461	1050	23.3	5250	2.7	1820	18.0
32462	1050	23.3	5250	2.7	1872	18.0
32463	1050	23.3	5250	2.7	1924	18.0
32464	1050	23.3	5250	2.7	1976	18.0
32465	1050	23.3	5250	2.7	2028	18.0
32466	1050	23.3	5250	2.7	2080	18.0
32467	1050	23.3	5250	2.7	2132	18.0
32468	1050	23.3	5250	2.7	2184	18.0
32469	1050	23.3	5250	2.7	2236	18.0
32470	1050	23.3	5250	2.7	2288	18.0
32471	1050	23.3	5250	2.7	2340	18.0
32472	1050	23.3	5250	2.7	2392	18.0
32473	1050	23.3	5250	2.7	2444	18.0
32474	1050	23.3	5250	2.7	2496	18.0
32534	1000	23.0	5000	2.8	2550	17.4
32515	1000	22.9	5000	2.8	1600	17.3
32818	800	22.2	4000	2.5	2560	17.2
32516	1000	22.2	5000	2.8	1650	16.6
32517	1000	22.2	5000	2.8	1700	16.6
32518	1000	22.2	5000	2.8	1750	16.6
32519	1000	22.2	5000	2.8	1800	16.6
32520	1000	22.2	5000	2.8	1850	16.6
32521	1000	22.2	5000	2.8	1900	16.6
32522	1000	22.2	5000	2.8	1950	16.6
32523	1000	22.2	5000	2.8	2000	16.6
32524	1000	22.2	5000	2.8	2050	16.6
32525	1000	22.2	5000	2.8	2100	16.6
32526	1000	22.2	5000	2.8	2150	16.6
32527	1000	22.2	5000	2.8	2200	16.6
32528	1000	22.2	5000	2.8	2250	16.6
32529	1000	22.2	5000	2.8	2300	16.6
32530	1000	22.2	5000	2.8	2350	16.6
32531	1000	22.2	5000	2.8	2400	16.6
32532	1000	22.2	5000	2.8	2450	16.6
32533	1000	22.2	5000	2.8	2500	16.6
32579	950	21.8	4750	2.9	1645	15.9
32598	950	21.6	4750	2.9	2538	15.7
33233	600	18.9	3000	1.7	3300	15.5
33078	650	20.8	3250	2.8	1920	15.3
32580	950	21.1	4750	2.9	1692	15.2
32581	950	21.1	4750	2.9	1739	15.2
32582	950	21.1	4750	2.9	1786	15.2
32583	950	21.1	4750	2.9	1833	15.2
32584	950	21.1	4750	2.9	1880	15.2
32585	950	21.1	4750	2.9	1927	15.2
32586	950	21.1	4750	2.9	1974	15.2
32587	950	21.1	4750	2.9	2021	15.2
32588	950	21.1	4750	2.9	2068	15.2
32589	950	21.1	4750	2.9	2115	15.2
32590	950	21.1	4750	2.9	2162	15.2
32591	950	21.1	4750	2.9	2209	15.2
32592	950	21.1	4750	2.9	2256	15.2
32593	950	21.1	4750	2.9	2303	15.2
32594	950	21.1	4750	2.9	2350	15.2
32595	950	21.1	4750	2.9	2397	15.2
32596	950	21.1	4750	2.9	2444	15.2
32597	950	21.1	4750	2.9	2491	15.2
33361	550	17.3	2750	1.5	3348	14.4
32665	900	20.5	4500	3.1	2520	14.2
32647	900	20.0	4500	3.1	1710	13.8
32648	900	20.0	4500	3.1	1755	13.8
32649	900	20.0	4500	3.1	1800	13.8
32650	900	20.0	4500	3.1	1845	13.8
32651	900	20.0	4500	3.1	1890	13.8
32652	900	20.0	4500	3.1	1935	13.8
32653	900	20.0	4500	3.1	1980	13.8
32654	900	20.0	4500	3.1	2025	13.8
32655	900	20.0	4500	3.1	2070	13.8
32656	900	20.0	4500	3.1	2115	13.8
32657	900	20.0	4500	3.1	2160	13.8
32658	900	20.0	4500	3.1	2205	13.8
32659	900	20.0	4500	3.1	2250	13.8
32660	900	20.0	4500	3.1	2295	13.8
32661	900	20.0	4500	3.1	2340	13.8
32662	900	20.0	4500	3.1	2385	13.8
32663	900	20.0	4500	3.1	2430	13.8
32664	900	20.0	4500	3.1	2475	13.8
33120	650	20.4	3250	3.7	3264	13.1
32738	850	19.3	4250	3.3	2520	12.7
32720	850	18.9	4250	3.3	1764	12.3
32721	850	18.9	4250	3.3	1806	12.3
32722	850	18.9	4250	3.3	1848	12.3
32723	850	18.9	4250	3.3	1890	12.3
32724	850	18.9	4250	3.3	1932	12.3
32725	850	18.9	4250	3.3	1974	12.3
32726	850	18.9	4250	3.3	2016	12.3
32727	850	18.9	4250	3.3	2058	12.3
32728	850	18.9	4250	3.3	2100	12.3
32729	850	18.9	4250	3.3	2142	12.3
32730	850	18.9	4250	3.3	2184	12.3
32731	850	18.9	4250	3.3	2226	12.3
32732	850	18.9	4250	3.3	2268	12.3
32733	850	18.9	4250	3.3	2310	12.3
32734	850	18.9	4250	3.3	2352	12.3
32735	850	18.9	4250	3.3	2394	12.3
32736	850	18.9	4250	3.3	2436	12.3
32737	850	18.9	4250	3.3	2478	12.3
33332	550	17.4	2750	2.7	2565	12.0
33098	650	18.1	3250	3.1	2560	11.9
33501	500	15.7	2500	2.0	3400	11.7
32799	800	18.3	4000	3.5	1800	11.3
32817	800	18.2	4000	3.5	2520	11.2
32800	800	17.8	4000	3.5	1840	10.8
32801	800	17.8	4000	3.5	1880	10.8
32802	800	17.8	4000	3.5	1920	10.8
32803	800	17.8	4000	3.5	1960	10.8
32804	800	17.8	4000	3.5	2000	10.8
32805	800	17.8	4000	3.5	2040	10.8
32806	800	17.8	4000	3.5	2080	10.8
32807	800	17.8	4000	3.5	2120	10.8
32808	800	17.8	4000	3.5	2160	10.8
32809	800	17.8	4000	3.5	2200	10.8
32810	800	17.8	4000	3.5	2240	10.8
32811	800	17.8	4000	3.5	2280	10.8
32812	800	17.8	4000	3.5	2320	10.8
32813	800	17.8	4000	3.5	2360	10.8
32814	800	17.8	4000	3.5	2400	10.8
32815	800	17.8	4000	3.5	2440	10.8
32816	800	17.8	4000	3.5	2480	10.8
32904	750	17.7	3750	3.6	2553	10.5
32996	700	17.3	3500	3.6	2555	10.2
32885	750	17.2	3750	3.7	1850	9.7
32903	750	17.0	3750	3.7	2516	9.5
32886	750	16.7	3750	3.7	1887	9.2
32887	750	16.7	3750	3.7	1924	9.2
32888	750	16.7	3750	3.7	1961	9.2
32889	750	16.7	3750	3.7	1998	9.2
32890	750	16.7	3750	3.7	2035	9.2
32891	750	16.7	3750	3.7	2072	9.2
32892	750	16.7	3750	3.7	2109	9.2
32893	750	16.7	3750	3.7	2146	9.2
32894	750	16.7	3750	3.7	2183	9.2
32895	750	16.7	3750	3.7	2220	9.2
32896	750	16.7	3750	3.7	2257	9.2
32897	750	16.7	3750	3.7	2294	9.2
32898	750	16.7	3750	3.7	2331	9.2
32899	750	16.7	3750	3.7	2368	9.2
32900	750	16.7	3750	3.7	2405	9.2
32901	750	16.7	3750	3.7	2442	9.2
32902	750	16.7	3750	3.7	2479	9.2
32977	700	16.9	3500	4.0	1890	8.9
32995	700	15.9	3500	4.0	2520	7.9
32978	700	15.6	3500	4.0	1925	7.6
32979	700	15.6	3500	4.0	1960	7.6
32980	700	15.6	3500	4.0	1995	7.6
32981	700	15.6	3500	4.0	2030	7.6
32982	700	15.6	3500	4.0	2065	7.6
32983	700	15.6	3500	4.0	2100	7.6
32984	700	15.6	3500	4.0	2135	7.6
32985	700	15.6	3500	4.0	2170	7.6
32986	700	15.6	3500	4.0	2205	7.6
32987	700	15.6	3500	4.0	2240	7.6
32988	700	15.6	3500	4.0	2275	7.6
32989	700	15.6	3500	4.0	2310	7.6
32990	700	15.6	3500	4.0	2345	7.6
32991	700	15.6	3500	4.0	2380	7.6
32992	700	15.6	3500	4.0	2415	7.6
32993	700	15.6	3500	4.0	2450	7.6
32994	700	15.6	3500	4.0	2485	7.6
33312	550	15.2	2750	4.2	2025	6.8
33079	650	14.9	3250	4.3	1952	6.3
33097	650	14.8	3250	4.3	2528	6.2
33080	650	14.4	3250	4.3	1984	5.8
33081	650	14.4	3250	4.3	2016	5.8
33082	650	14.4	3250	4.3	2048	5.8
33083	650	14.4	3250	4.3	2080	5.8
33084	650	14.4	3250	4.3	2112	5.8
33085	650	14.4	3250	4.3	2144	5.8
33086	650	14.4	3250	4.3	2176	5.8
33087	650	14.4	3250	4.3	2208	5.8
33088	650	14.4	3250	4.3	2240	5.8
33089	650	14.4	3250	4.3	2272	5.8
33090	650	14.4	3250	4.3	2304	5.8
33091	650	14.4	3250	4.3	2336	5.8
33092	650	14.4	3250	4.3	2368	5.8
33093	650	14.4	3250	4.3	2400	5.8
33094	650	14.4	3250	4.3	2432	5.8
33095	650	14.4	3250	4.3	2464	5.8
33096	650	14.4	3250	4.3	2496	5.8
33189	600	14.6	3000	4.7	1980	5.2
33448	500	13.8	2500	4.6	2075	4.6
33208	600	13.8	3000	4.7	2550	4.5
33207	600	13.6	3000	4.7	2520	4.3
33190	600	13.3	3000	4.7	2010	4.0
33191	600	13.3	3000	4.7	2040	4.0
33192	600	13.3	3000	4.7	2070	4.0
33193	600	13.3	3000	4.7	2100	4.0
33194	600	13.3	3000	4.7	2130	4.0
33195	600	13.3	3000	4.7	2160	4.0
33196	600	13.3	3000	4.7	2190	4.0
33197	600	13.3	3000	4.7	2220	4.0
33198	600	13.3	3000	4.7	2250	4.0
33199	600	13.3	3000	4.7	2280	4.0
33200	600	13.3	3000	4.7	2310	4.0
33201	600	13.3	3000	4.7	2340	4.0
33202	600	13.3	3000	4.7	2370	4.0
33203	600	13.3	3000	4.7	2400	4.0
33204	600	13.3	3000	4.7	2430	4.0
33205	600	13.3	3000	4.7	2460	4.0
33206	600	13.3	3000	4.7	2490	4.0

Overall score = score_ratio - 2 * repetitive DNA content