Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign60 - back to list

Summary
Image
Unique putative probes (303)
Putative probes (133)
Redundant probes (209)

Summary

`./analyse_probe_search_results : Fri Oct 7 10:45:00 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign60
  assembly      : NCBIM34
  bias          : 5prime
  chromosome    : 11
  id            : 51
  strand        : 1
  -------------

Design window length: 3001bp

All jobs for probe design are successful - total: 46
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 907 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 907/907
Putative probes that are unique                 : 303/907
Putative probes exceeding selection criteria    : 342/907 (but not unique)
Putative probes with successfully-picked primers: 330/645

Identified 209 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
50901	750	3750	-	666
50902	750	3750	-	703
50963	700	3500	-	665
50964	700	3500	-	700
50965	700	3500	-	735
51031	650	3250	-	672
51032	650	3250	-	704
51033	650	3250	-	736
51034	650	3250	-	768
51035	650	3250	-	800
51065	650	3250	-	1760
51106	600	3000	-	660
51107	600	3000	-	690
51108	600	3000	-	720
51109	600	3000	-	750
51110	600	3000	-	780
51111	600	3000	-	810
51112	600	3000	-	840
51143	600	3000	-	1770
51144	600	3000	-	1800
51189	550	2750	-	648
51190	550	2750	-	675
51191	550	2750	-	702
51192	550	2750	-	729
51193	550	2750	-	756
51194	550	2750	-	783
51195	550	2750	-	810
51196	550	2750	-	837
51197	550	2750	-	864
51198	550	2750	-	891
51231	550	2750	-	1782
51232	550	2750	-	1809
51233	550	2750	-	1836
51234	550	2750	-	1863
51282	500	2500	-	650
51283	500	2500	-	675
51284	500	2500	-	700
51285	500	2500	-	725
51286	500	2500	-	750
51287	500	2500	-	775
51288	500	2500	-	800
51289	500	2500	-	825
51290	500	2500	-	850
51291	500	2500	-	875
51292	500	2500	-	900
51293	500	2500	-	925
51294	500	2500	-	950
51327	500	2500	-	1775
51328	500	2500	-	1800
51329	500	2500	-	1825
51330	500	2500	-	1850
51331	500	2500	-	1875
51332	500	2500	-	1900
51333	500	2500	0.4	1925
50843	800	4000	1.0	640
51145	600	3000	1.2	1830
50651	1000	5000	1.2	400
50652	1000	5000	1.2	450
51030	650	3250	1.2	640
50692	950	4750	1.3	376
50693	950	4750	1.3	423
50694	950	4750	1.3	470
50737	900	4500	1.3	405
50738	900	4500	1.3	450
50739	900	4500	1.3	495
50740	900	4500	1.3	540
50784	850	4250	1.4	378
50785	850	4250	1.4	420
50786	850	4250	1.4	462
50787	850	4250	1.4	504
50788	850	4250	1.4	546
50789	850	4250	1.4	588
50612	1050	5250	1.4	364
50837	800	4000	1.5	400
50838	800	4000	1.5	440
50839	800	4000	1.5	480
50840	800	4000	1.5	520
50841	800	4000	1.5	560
50842	800	4000	1.5	600
50893	750	3750	1.6	370
50894	750	3750	1.6	407
50895	750	3750	1.6	444
50896	750	3750	1.6	481
50897	750	3750	1.6	518
50898	750	3750	1.6	555
50899	750	3750	1.6	592
50900	750	3750	1.6	629
50955	700	3500	1.7	385
50956	700	3500	1.7	420
50957	700	3500	1.7	455
50958	700	3500	1.7	490
50959	700	3500	1.7	525
50960	700	3500	1.7	560
50961	700	3500	1.7	595
50962	700	3500	1.7	630
50450	1300	6500	1.8	0
50451	1300	6500	1.8	65
50452	1300	6500	1.8	130
51022	650	3250	1.8	384
51023	650	3250	1.8	416
51024	650	3250	1.8	448
51025	650	3250	1.8	480
51026	650	3250	1.8	512
51027	650	3250	1.8	544
51028	650	3250	1.8	576
51029	650	3250	1.8	608
50966	700	3500	1.9	770
50477	1250	6250	1.9	0
50478	1250	6250	1.9	62
50479	1250	6250	1.9	124
50480	1250	6250	1.9	186
50506	1200	6000	2.0	0
50507	1200	6000	2.0	60
50508	1200	6000	2.0	120
50509	1200	6000	2.0	180
50510	1200	6000	2.0	240
50613	1050	5250	2.0	416
51084	600	3000	2.0	0
51085	600	3000	2.0	30
51097	600	3000	2.0	390
51098	600	3000	2.0	420
51099	600	3000	2.0	450
51100	600	3000	2.0	480
51101	600	3000	2.0	510
51102	600	3000	2.0	540
51103	600	3000	2.0	570
51104	600	3000	2.0	600
51105	600	3000	2.0	630
51199	550	2750	2.0	918
50537	1150	5750	2.1	0
50538	1150	5750	2.1	57
50539	1150	5750	2.1	114
50540	1150	5750	2.1	171
50541	1150	5750	2.1	228
50542	1150	5750	2.1	285
50736	900	4500	2.1	360
51113	600	3000	2.2	870
50570	1100	5500	2.2	0
50571	1100	5500	2.2	55
50572	1100	5500	2.2	110
50573	1100	5500	2.2	165
50574	1100	5500	2.2	220
50575	1100	5500	2.2	275
50576	1100	5500	2.2	330
51165	550	2750	2.2	0
51166	550	2750	2.2	27
51167	550	2750	2.2	54
51168	550	2750	2.2	81
51179	550	2750	2.2	378
51180	550	2750	2.2	405
51181	550	2750	2.2	432
51182	550	2750	2.2	459
51183	550	2750	2.2	486
51184	550	2750	2.2	513
51185	550	2750	2.2	540
51186	550	2750	2.2	567
51187	550	2750	2.2	594
51188	550	2750	2.2	621
50605	1050	5250	2.3	0
50606	1050	5250	2.3	52
50607	1050	5250	2.3	104
50608	1050	5250	2.3	156
50609	1050	5250	2.3	208
50610	1050	5250	2.3	260
50611	1050	5250	2.3	312
50695	950	4750	2.3	517
50836	800	4000	2.4	360
50643	1000	5000	2.4	0
50644	1000	5000	2.4	50
50645	1000	5000	2.4	100
50646	1000	5000	2.4	150
50647	1000	5000	2.4	200
50648	1000	5000	2.4	250
50649	1000	5000	2.4	300
50650	1000	5000	2.4	350
51256	500	2500	2.4	0
51257	500	2500	2.4	25
51258	500	2500	2.4	50
51259	500	2500	2.4	75
51260	500	2500	2.4	100
51261	500	2500	2.4	125
51271	500	2500	2.4	375
51272	500	2500	2.4	400
51273	500	2500	2.4	425
51274	500	2500	2.4	450
51275	500	2500	2.4	475
51276	500	2500	2.4	500
51277	500	2500	2.4	525
51278	500	2500	2.4	550
51279	500	2500	2.4	575
51280	500	2500	2.4	600
51281	500	2500	2.4	625
50684	950	4750	2.5	0
50685	950	4750	2.5	47
50686	950	4750	2.5	94
50687	950	4750	2.5	141
50688	950	4750	2.5	188
50689	950	4750	2.5	235
50690	950	4750	2.5	282
50691	950	4750	2.5	329
50728	900	4500	2.7	0
50729	900	4500	2.7	45
50730	900	4500	2.7	90
50731	900	4500	2.7	135
50732	900	4500	2.7	180
50733	900	4500	2.7	225
50734	900	4500	2.7	270
50735	900	4500	2.7	315
50775	850	4250	2.8	0
50776	850	4250	2.8	42
50777	850	4250	2.8	84
50778	850	4250	2.8	126
50779	850	4250	2.8	168
50780	850	4250	2.8	210
50781	850	4250	2.8	252
50782	850	4250	2.8	294
50783	850	4250	2.8	336
50844	800	4000	2.9	680
51066	650	3250	2.9	1792
50827	800	4000	3.0	0
50828	800	4000	3.0	40
50829	800	4000	3.0	80
50830	800	4000	3.0	120
50831	800	4000	3.0	160
50832	800	4000	3.0	200
50833	800	4000	3.0	240
50834	800	4000	3.0	280
50835	800	4000	3.0	320
51235	550	2750	3.1	1890
51096	600	3000	3.2	360
50883	750	3750	3.2	0
50884	750	3750	3.2	37
50885	750	3750	3.2	74
50886	750	3750	3.2	111
50887	750	3750	3.2	148
50888	750	3750	3.2	185
50889	750	3750	3.2	222
50890	750	3750	3.2	259
50891	750	3750	3.2	296
50892	750	3750	3.2	333
50944	700	3500	3.4	0
50945	700	3500	3.4	35
50946	700	3500	3.4	70
50947	700	3500	3.4	105
50948	700	3500	3.4	140
50949	700	3500	3.4	175
50950	700	3500	3.4	210
50951	700	3500	3.4	245
50952	700	3500	3.4	280
50953	700	3500	3.4	315
50954	700	3500	3.4	350
51295	500	2500	3.6	975
50577	1100	5500	3.6	385
51010	650	3250	3.7	0
51011	650	3250	3.7	32
51012	650	3250	3.7	64
51013	650	3250	3.7	96
51014	650	3250	3.7	128
51015	650	3250	3.7	160
51016	650	3250	3.7	192
51017	650	3250	3.7	224
51018	650	3250	3.7	256
51019	650	3250	3.7	288
51020	650	3250	3.7	320
51021	650	3250	3.7	352
51036	650	3250	3.8	832
51086	600	3000	4.0	60
51087	600	3000	4.0	90
51088	600	3000	4.0	120
51089	600	3000	4.0	150
51090	600	3000	4.0	180
51091	600	3000	4.0	210
51092	600	3000	4.0	240
51093	600	3000	4.0	270
51094	600	3000	4.0	300
51095	600	3000	4.0	330
50790	850	4250	4.1	630
50481	1250	6250	4.2	248
51169	550	2750	4.4	108
51170	550	2750	4.4	135
51171	550	2750	4.4	162
51172	550	2750	4.4	189
51173	550	2750	4.4	216
51174	550	2750	4.4	243
51175	550	2750	4.4	270
51176	550	2750	4.4	297
51177	550	2750	4.4	324
51178	550	2750	4.4	351
50741	900	4500	4.4	585
51262	500	2500	4.8	150
51263	500	2500	4.8	175
51264	500	2500	4.8	200
51265	500	2500	4.8	225
51266	500	2500	4.8	250
51267	500	2500	4.8	275
51268	500	2500	4.8	300
51269	500	2500	4.8	325
51270	500	2500	4.8	350
51334	500	2500	5.4	1950
51146	600	3000	6.2	1860
51067	650	3250	7.8	1824
51236	550	2750	8.0	1917
51335	500	2500	10.4	1975

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
50456	1300	41.1	6500	3.1	390	35.0
50457	1300	41.1	6500	3.1	455	35.0
50453	1300	41.9	6500	4.0	195	33.9
50483	1250	39.6	6250	3.2	372	33.2
50454	1300	41.1	6500	4.0	260	33.1
50455	1300	41.1	6500	4.0	325	33.1
50460	1300	36.7	6500	2.2	650	32.4
50461	1300	36.7	6500	2.2	715	32.4
50462	1300	36.7	6500	2.2	780	32.4
50463	1300	36.7	6500	2.2	845	32.4
50464	1300	36.7	6500	2.2	910	32.4
50465	1300	36.7	6500	2.2	975	32.4
50466	1300	36.7	6500	2.2	1040	32.4
50467	1300	36.7	6500	2.2	1105	32.4
50513	1200	38.0	6000	3.3	420	31.3
50458	1300	36.7	6500	3.1	520	30.6
50459	1300	36.7	6500	3.1	585	30.6
50512	1200	38.0	6000	3.9	360	30.1
50496	1250	35.3	6250	2.6	1178	30.0
50582	1100	34.8	5500	2.5	660	29.7
50544	1150	36.4	5750	3.5	399	29.4
50545	1150	36.4	5750	3.5	456	29.4
50517	1200	33.9	6000	2.3	660	29.2
50578	1100	35.7	5500	3.6	440	28.4
50618	1050	33.2	5250	2.7	676	27.9
50619	1050	33.2	5250	2.7	728	27.9
50549	1150	32.5	5750	2.4	684	27.6
50579	1100	34.8	5500	3.6	495	27.5
50614	1050	34.1	5250	3.8	468	26.5
50656	1000	31.6	5000	2.8	650	26.0
50657	1000	31.6	5000	2.8	700	26.0
50658	1000	31.6	5000	2.8	750	26.0
50659	1000	31.6	5000	2.8	800	26.0
50583	1100	31.1	5500	2.5	715	26.0
50584	1100	31.1	5500	2.5	770	26.0
50594	1100	31.1	5500	2.5	1320	26.0
50615	1050	33.2	5250	3.8	520	25.6
50616	1050	33.2	5250	3.8	572	25.6
50761	900	25.4	4500	-	1485	25.4
50653	1000	32.5	5000	4.0	500	24.5
50620	1050	29.7	5250	2.7	780	24.3
50621	1050	29.7	5250	2.7	832	24.3
50698	950	30.1	4750	2.9	658	24.2
50699	950	30.1	4750	2.9	705	24.2
50700	950	30.1	4750	2.9	752	24.2
50701	950	30.1	4750	2.9	799	24.2
50702	950	30.1	4750	2.9	846	24.2
50811	850	24.0	4250	-	1512	24.0
50812	850	24.0	4250	-	1554	24.0
50762	900	25.4	4500	0.8	1530	23.9
50654	1000	31.6	5000	4.0	550	23.6
50655	1000	31.6	5000	4.0	600	23.6
50660	1000	28.2	5000	2.8	850	22.6
50865	800	22.6	4000	-	1520	22.6
50866	800	22.6	4000	-	1560	22.6
50867	800	22.6	4000	-	1600	22.6
50743	900	28.5	4500	3.1	675	22.3
50744	900	28.5	4500	3.1	720	22.3
50864	800	22.6	4000	0.6	1480	21.3
50924	750	21.2	3750	-	1517	21.2
50925	750	21.2	3750	-	1554	21.2
50926	750	21.2	3750	-	1591	21.2
50927	750	21.2	3750	-	1628	21.2
50928	750	21.2	3750	-	1665	21.2
50791	850	27.6	4250	3.3	672	21.0
50703	950	26.8	4750	2.9	893	20.9
50810	850	24.0	4250	1.8	1470	20.5
50792	850	26.9	4250	3.3	714	20.3
50793	850	26.9	4250	3.3	756	20.3
50716	950	26.8	4750	3.3	1504	20.3
50923	750	21.2	3750	0.7	1480	19.9
50987	700	19.8	3500	-	1505	19.8
50988	700	19.8	3500	-	1540	19.8
50989	700	19.8	3500	-	1575	19.8
50990	700	19.8	3500	-	1610	19.8
50991	700	19.8	3500	-	1645	19.8
50992	700	19.8	3500	-	1680	19.8
50993	700	19.8	3500	-	1715	19.8
50845	800	26.0	4000	3.5	720	19.0
50813	850	24.0	4250	2.7	1596	18.6
51057	650	18.4	3250	-	1504	18.4
51058	650	18.4	3250	-	1536	18.4
51059	650	18.4	3250	-	1568	18.4
51060	650	18.4	3250	-	1600	18.4
51061	650	18.4	3250	-	1632	18.4
51062	650	18.4	3250	-	1664	18.4
51063	650	18.4	3250	-	1696	18.4
51064	650	18.4	3250	-	1728	18.4
50868	800	22.6	4000	2.1	1640	18.3
50846	800	25.3	4000	3.5	760	18.3
50847	800	25.3	4000	3.5	800	18.3
51230	550	18.2	2750	-	1755	18.2
51142	600	17.6	3000	-	1740	17.6
50903	750	24.5	3750	3.7	740	17.0
51134	600	16.9	3000	-	1500	16.9
51135	600	16.9	3000	-	1530	16.9
51136	600	16.9	3000	-	1560	16.9
51137	600	16.9	3000	-	1590	16.9
51138	600	16.9	3000	-	1620	16.9
51139	600	16.9	3000	-	1650	16.9
51140	600	16.9	3000	-	1680	16.9
51141	600	16.9	3000	-	1710	16.9
50904	750	24.4	3750	3.7	777	16.9
50905	750	23.7	3750	3.7	814	16.3
50906	750	23.7	3750	3.7	851	16.3
51326	500	15.8	2500	-	1750	15.8
51220	550	15.5	2750	-	1485	15.5
51221	550	15.5	2750	-	1512	15.5
51222	550	15.5	2750	-	1539	15.5
51223	550	15.5	2750	-	1566	15.5
51224	550	15.5	2750	-	1593	15.5
51225	550	15.5	2750	-	1620	15.5
51226	550	15.5	2750	-	1647	15.5
51227	550	15.5	2750	-	1674	15.5
51228	550	15.5	2750	-	1701	15.5
51229	550	15.5	2750	-	1728	15.5
50986	700	19.8	3500	2.1	1470	15.5
50967	700	22.7	3500	4.0	805	14.7
50968	700	22.7	3500	4.0	840	14.7
51056	650	18.4	3250	2.0	1472	14.4
50969	700	22.2	3500	4.0	875	14.2
50970	700	22.2	3500	4.0	910	14.2
51316	500	14.1	2500	-	1500	14.1
51317	500	14.1	2500	-	1525	14.1
51318	500	14.1	2500	-	1550	14.1
51319	500	14.1	2500	-	1575	14.1
51320	500	14.1	2500	-	1600	14.1
51321	500	14.1	2500	-	1625	14.1
51322	500	14.1	2500	-	1650	14.1
51323	500	14.1	2500	-	1675	14.1
51324	500	14.1	2500	-	1700	14.1
51325	500	14.1	2500	-	1725	14.1
51315	500	14.1	2500	2.0	1475	10.1

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
50484	1250	39.6	6250	3.2	434	33.2
50485	1250	39.6	6250	3.2	496	33.2
50486	1250	39.1	6250	3.2	558	32.7
50514	1200	38.0	6000	3.3	480	31.3
50515	1200	38.0	6000	3.3	540	31.3
50516	1200	38.0	6000	3.3	600	31.3
50482	1250	39.6	6250	4.2	310	31.2
50488	1250	35.3	6250	2.2	682	30.8
50489	1250	35.3	6250	2.2	744	30.8
50490	1250	35.3	6250	2.2	806	30.8
50491	1250	35.3	6250	2.2	868	30.8
50492	1250	35.3	6250	2.2	930	30.8
50493	1250	35.3	6250	2.2	992	30.8
50494	1250	35.3	6250	2.2	1054	30.8
50495	1250	35.3	6250	2.2	1116	30.8
50511	1200	39.0	6000	4.3	300	30.3
50546	1150	36.4	5750	3.5	513	29.4
50547	1150	36.4	5750	3.5	570	29.4
50548	1150	36.4	5750	3.5	627	29.4
50518	1200	33.9	6000	2.3	720	29.2
50519	1200	33.9	6000	2.3	780	29.2
50520	1200	33.9	6000	2.3	840	29.2
50521	1200	33.9	6000	2.3	900	29.2
50522	1200	33.9	6000	2.3	960	29.2
50523	1200	33.9	6000	2.3	1020	29.2
50524	1200	33.9	6000	2.3	1080	29.2
50525	1200	33.9	6000	2.3	1140	29.2
50526	1200	33.9	6000	2.3	1200	29.2
50487	1250	35.3	6250	3.2	620	28.9
50543	1150	37.3	5750	4.5	342	28.3
50550	1150	32.5	5750	2.4	741	27.6
50551	1150	32.5	5750	2.4	798	27.6
50552	1150	32.5	5750	2.4	855	27.6
50553	1150	32.5	5750	2.4	912	27.6
50554	1150	32.5	5750	2.4	969	27.6
50555	1150	32.5	5750	2.4	1026	27.6
50556	1150	32.5	5750	2.4	1083	27.6
50557	1150	32.5	5750	2.4	1140	27.6
50558	1150	32.5	5750	2.4	1197	27.6
50559	1150	32.5	5750	2.4	1254	27.6
50580	1100	34.8	5500	3.6	550	27.5
50581	1100	34.8	5500	3.6	605	27.5
50585	1100	31.1	5500	2.5	825	26.0
50586	1100	31.1	5500	2.5	880	26.0
50587	1100	31.1	5500	2.5	935	26.0
50588	1100	31.1	5500	2.5	990	26.0
50589	1100	31.1	5500	2.5	1045	26.0
50590	1100	31.1	5500	2.5	1100	26.0
50591	1100	31.1	5500	2.5	1155	26.0
50592	1100	31.1	5500	2.5	1210	26.0
50593	1100	31.1	5500	2.5	1265	26.0
50617	1050	33.2	5250	3.8	624	25.6
50622	1050	29.7	5250	2.7	884	24.3
50623	1050	29.7	5250	2.7	936	24.3
50624	1050	29.7	5250	2.7	988	24.3
50625	1050	29.7	5250	2.7	1040	24.3
50626	1050	29.7	5250	2.7	1092	24.3
50627	1050	29.7	5250	2.7	1144	24.3
50628	1050	29.7	5250	2.7	1196	24.3
50629	1050	29.7	5250	2.7	1248	24.3
50630	1050	29.7	5250	2.7	1300	24.3
50631	1050	29.7	5250	2.7	1352	24.3
50661	1000	28.2	5000	2.8	900	22.6
50662	1000	28.2	5000	2.8	950	22.6
50663	1000	28.2	5000	2.8	1000	22.6
50664	1000	28.2	5000	2.8	1050	22.6
50665	1000	28.2	5000	2.8	1100	22.6
50666	1000	28.2	5000	2.8	1150	22.6
50667	1000	28.2	5000	2.8	1200	22.6
50668	1000	28.2	5000	2.8	1250	22.6
50669	1000	28.2	5000	2.8	1300	22.6
50670	1000	28.2	5000	2.8	1350	22.6
50671	1000	28.2	5000	2.8	1400	22.6
50696	950	30.8	4750	4.2	564	22.4
50745	900	28.5	4500	3.1	765	22.3
50746	900	28.5	4500	3.1	810	22.3
50747	900	28.5	4500	3.1	855	22.3
50748	900	28.5	4500	3.1	900	22.3
50697	950	30.1	4750	4.2	611	21.6
50704	950	26.8	4750	2.9	940	20.9
50705	950	26.8	4750	2.9	987	20.9
50706	950	26.8	4750	2.9	1034	20.9
50707	950	26.8	4750	2.9	1081	20.9
50708	950	26.8	4750	2.9	1128	20.9
50709	950	26.8	4750	2.9	1175	20.9
50710	950	26.8	4750	2.9	1222	20.9
50711	950	26.8	4750	2.9	1269	20.9
50712	950	26.8	4750	2.9	1316	20.9
50713	950	26.8	4750	2.9	1363	20.9
50714	950	26.8	4750	2.9	1410	20.9
50715	950	26.8	4750	2.9	1457	20.9
50742	900	29.2	4500	4.4	630	20.3
50794	850	26.9	4250	3.3	798	20.3
50795	850	26.9	4250	3.3	840	20.3
50796	850	26.9	4250	3.3	882	20.3
50797	850	26.9	4250	3.3	924	20.3
50749	900	25.4	4500	3.1	945	19.2
50750	900	25.4	4500	3.1	990	19.2
50751	900	25.4	4500	3.1	1035	19.2
50752	900	25.4	4500	3.1	1080	19.2
50753	900	25.4	4500	3.1	1125	19.2
50754	900	25.4	4500	3.1	1170	19.2
50755	900	25.4	4500	3.1	1215	19.2
50756	900	25.4	4500	3.1	1260	19.2
50757	900	25.4	4500	3.1	1305	19.2
50758	900	25.4	4500	3.1	1350	19.2
50759	900	25.4	4500	3.1	1395	19.2
50760	900	25.4	4500	3.1	1440	19.2
50848	800	25.3	4000	3.5	840	18.3
50849	800	25.3	4000	3.5	880	18.3
50850	800	25.3	4000	3.5	920	18.3
50851	800	25.3	4000	3.5	960	18.3
50852	800	25.3	4000	3.5	1000	18.3
50798	850	24.0	4250	3.3	966	17.4
50799	850	24.0	4250	3.3	1008	17.4
50800	850	24.0	4250	3.3	1050	17.4
50801	850	24.0	4250	3.3	1092	17.4
50802	850	24.0	4250	3.3	1134	17.4
50803	850	24.0	4250	3.3	1176	17.4
50804	850	24.0	4250	3.3	1218	17.4
50805	850	24.0	4250	3.3	1260	17.4
50806	850	24.0	4250	3.3	1302	17.4
50807	850	24.0	4250	3.3	1344	17.4
50808	850	24.0	4250	3.3	1386	17.4
50809	850	24.0	4250	3.3	1428	17.4
50907	750	23.7	3750	3.7	888	16.3
50908	750	23.7	3750	3.7	925	16.3
50909	750	23.7	3750	3.7	962	16.3
50910	750	23.7	3750	3.7	999	16.3
50911	750	23.7	3750	3.7	1036	16.3
50853	800	22.6	4000	3.5	1040	15.6
50854	800	22.6	4000	3.5	1080	15.6
50855	800	22.6	4000	3.5	1120	15.6
50856	800	22.6	4000	3.5	1160	15.6
50857	800	22.6	4000	3.5	1200	15.6
50858	800	22.6	4000	3.5	1240	15.6
50859	800	22.6	4000	3.5	1280	15.6
50860	800	22.6	4000	3.5	1320	15.6
50861	800	22.6	4000	3.5	1360	15.6
50862	800	22.6	4000	3.5	1400	15.6
50863	800	22.6	4000	3.5	1440	15.6
50994	700	22.2	3500	3.9	1750	14.4
50971	700	22.2	3500	4.0	945	14.2
50972	700	22.2	3500	4.0	980	14.2
50973	700	22.2	3500	4.0	1015	14.2
50974	700	22.2	3500	4.0	1050	14.2
50975	700	22.2	3500	4.0	1085	14.2
50912	750	21.2	3750	3.7	1073	13.7
50913	750	21.2	3750	3.7	1110	13.7
50914	750	21.2	3750	3.7	1147	13.7
50915	750	21.2	3750	3.7	1184	13.7
50916	750	21.2	3750	3.7	1221	13.7
50917	750	21.2	3750	3.7	1258	13.7
50918	750	21.2	3750	3.7	1295	13.7
50919	750	21.2	3750	3.7	1332	13.7
50920	750	21.2	3750	3.7	1369	13.7
50921	750	21.2	3750	3.7	1406	13.7
50922	750	21.2	3750	3.7	1443	13.7
50929	750	21.2	3750	3.9	1702	13.5
51037	650	21.1	3250	4.3	864	12.5
51038	650	20.6	3250	4.3	896	12.0
51039	650	20.6	3250	4.3	928	12.0
51040	650	20.6	3250	4.3	960	12.0
51041	650	20.6	3250	4.3	992	12.0
51042	650	20.6	3250	4.3	1024	12.0
51043	650	20.6	3250	4.3	1056	12.0
51044	650	20.6	3250	4.3	1088	12.0
51045	650	20.6	3250	4.3	1120	12.0
51046	650	20.6	3250	4.3	1152	12.0
51133	600	16.9	3000	2.5	1470	11.9
50976	700	19.8	3500	4.0	1120	11.8
50977	700	19.8	3500	4.0	1155	11.8
50978	700	19.8	3500	4.0	1190	11.8
50979	700	19.8	3500	4.0	1225	11.8
50980	700	19.8	3500	4.0	1260	11.8
50981	700	19.8	3500	4.0	1295	11.8
50982	700	19.8	3500	4.0	1330	11.8
50983	700	19.8	3500	4.0	1365	11.8
50984	700	19.8	3500	4.0	1400	11.8
50985	700	19.8	3500	4.0	1435	11.8
51114	600	19.5	3000	4.7	900	10.1
51115	600	19.5	3000	4.7	930	10.1
51047	650	18.4	3250	4.3	1184	9.7
51048	650	18.4	3250	4.3	1216	9.7
51049	650	18.4	3250	4.3	1248	9.7
51050	650	18.4	3250	4.3	1280	9.7
51051	650	18.4	3250	4.3	1312	9.7
51052	650	18.4	3250	4.3	1344	9.7
51053	650	18.4	3250	4.3	1376	9.7
51054	650	18.4	3250	4.3	1408	9.7
51055	650	18.4	3250	4.3	1440	9.7
51116	600	19.0	3000	4.7	960	9.7
51117	600	19.0	3000	4.7	990	9.7
51118	600	19.0	3000	4.7	1020	9.7
51119	600	19.0	3000	4.7	1050	9.7
51120	600	19.0	3000	4.7	1080	9.7
51121	600	19.0	3000	4.7	1110	9.7
51122	600	19.0	3000	4.7	1140	9.7
51123	600	19.0	3000	4.7	1170	9.7
51124	600	19.0	3000	4.7	1200	9.7
51125	600	16.9	3000	4.7	1230	7.6
51126	600	16.9	3000	4.7	1260	7.6
51127	600	16.9	3000	4.7	1290	7.6
51128	600	16.9	3000	4.7	1320	7.6
51129	600	16.9	3000	4.7	1350	7.6
51130	600	16.9	3000	4.7	1380	7.6
51131	600	16.9	3000	4.7	1410	7.6
51132	600	16.9	3000	4.7	1440	7.6
51219	550	15.5	2750	4.9	1458	5.7

Overall score = score_ratio - 2 * repetitive DNA content