Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign61 - back to list

Summary
Image
Unique putative probes (629)
Putative probes (134)
Redundant probes (97)

Summary

`./analyse_probe_search_results : Fri Oct 7 10:46:11 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign61
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 11
  id            : 52
  strand        : 1
  -------------

Design window length: 3001bp

All jobs for probe design are successful - total: 46
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 907 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 907/907
Putative probes that are unique                 : 629/907
Putative probes exceeding selection criteria    : 231/907 (but not unique)
Putative probes with successfully-picked primers: 611/860

Identified 97 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
51380	1300	6500	-	1495
51408	1250	6250	-	1488
51409	1250	6250	-	1550
51438	1200	6000	-	1500
51439	1200	6000	-	1560
51440	1200	6000	-	1620
51470	1150	5750	-	1482
51471	1150	5750	-	1539
51472	1150	5750	-	1596
51473	1150	5750	-	1653
51504	1100	5500	-	1485
51505	1100	5500	-	1540
51506	1100	5500	-	1595
51507	1100	5500	-	1650
51508	1100	5500	-	1705
51541	1050	5250	-	1508
51542	1050	5250	-	1560
51543	1050	5250	-	1612
51544	1050	5250	-	1664
51545	1050	5250	-	1716
51546	1050	5250	-	1768
51580	1000	5000	-	1500
51581	1000	5000	-	1550
51582	1000	5000	-	1600
51583	1000	5000	-	1650
51584	1000	5000	-	1700
51585	1000	5000	-	1750
51586	1000	5000	-	1800
51623	950	4750	-	1504
51624	950	4750	-	1551
51625	950	4750	-	1598
51626	950	4750	-	1645
51627	950	4750	-	1692
51628	950	4750	-	1739
51629	950	4750	-	1786
51630	950	4750	-	1833
51631	950	4750	-	1880
51646	900	4500	-	495
51647	900	4500	-	540
51668	900	4500	-	1485
51669	900	4500	-	1530
51670	900	4500	-	1575
51671	900	4500	-	1620
51672	900	4500	-	1665
51673	900	4500	-	1710
51674	900	4500	-	1755
51675	900	4500	-	1800
51676	900	4500	-	1845
51677	900	4500	-	1890
51678	900	4500	-	1935
51694	850	4250	-	504
51695	850	4250	-	546
51696	850	4250	-	588
51717	850	4250	-	1470
51718	850	4250	-	1512
51719	850	4250	-	1554
51720	850	4250	-	1596
51721	850	4250	-	1638
51722	850	4250	-	1680
51723	850	4250	-	1722
51724	850	4250	-	1764
51725	850	4250	-	1806
51726	850	4250	-	1848
51727	850	4250	-	1890
51728	850	4250	-	1932
51729	850	4250	-	1974
51747	800	4000	-	520
51748	800	4000	-	560
51749	800	4000	-	600
51750	800	4000	-	640
51771	800	4000	-	1480
51772	800	4000	-	1520
51773	800	4000	-	1560
51774	800	4000	-	1600
51775	800	4000	-	1640
51776	800	4000	-	1680
51777	800	4000	-	1720
51778	800	4000	-	1760
51779	800	4000	-	1800
51780	800	4000	-	1840
51781	800	4000	-	1880
51782	800	4000	-	1920
51783	800	4000	-	1960
51784	800	4000	-	2000
51785	800	4000	-	2040
51804	750	3750	-	518
51805	750	3750	-	555
51806	750	3750	-	592
51807	750	3750	-	629
51808	750	3750	-	666
51830	750	3750	-	1480
51831	750	3750	-	1517
51832	750	3750	-	1554
51833	750	3750	-	1591
51834	750	3750	-	1628
51835	750	3750	-	1665
51836	750	3750	-	1702
51837	750	3750	-	1739
51838	750	3750	-	1776
51839	750	3750	-	1813
51840	750	3750	-	1850
51841	750	3750	-	1887
51842	750	3750	-	1924
51843	750	3750	-	1961
51844	750	3750	-	1998
51845	750	3750	-	2035
51846	750	3750	-	2072
51865	700	3500	-	490
51866	700	3500	-	525
51867	700	3500	-	560
51868	700	3500	-	595
51869	700	3500	-	630
51870	700	3500	-	665
51871	700	3500	-	700
51872	700	3500	-	735
51893	700	3500	-	1470
51894	700	3500	-	1505
51895	700	3500	-	1540
51896	700	3500	-	1575
51897	700	3500	-	1610
51898	700	3500	-	1645
51899	700	3500	-	1680
51900	700	3500	-	1715
51901	700	3500	-	1750
51902	700	3500	-	1785
51903	700	3500	-	1820
51904	700	3500	-	1855
51905	700	3500	-	1890
51906	700	3500	-	1925
51907	700	3500	-	1960
51908	700	3500	-	1995
51909	700	3500	-	2030
51910	700	3500	-	2065
51911	700	3500	-	2100
51912	700	3500	-	2135
51933	650	3250	-	512
51934	650	3250	-	544
51935	650	3250	-	576
51936	650	3250	-	608
51937	650	3250	-	640
51938	650	3250	-	672
51939	650	3250	-	704
51940	650	3250	-	736
51941	650	3250	-	768
51963	650	3250	-	1472
51964	650	3250	-	1504
51965	650	3250	-	1536
51966	650	3250	-	1568
51967	650	3250	-	1600
51968	650	3250	-	1632
51969	650	3250	-	1664
51970	650	3250	-	1696
51971	650	3250	-	1728
51972	650	3250	-	1760
51973	650	3250	-	1792
51974	650	3250	-	1824
51975	650	3250	-	1856
51976	650	3250	-	1888
51977	650	3250	-	1920
51978	650	3250	-	1952
51979	650	3250	-	1984
51980	650	3250	-	2016
51981	650	3250	-	2048
51982	650	3250	-	2080
51983	650	3250	-	2112
51984	650	3250	-	2144
51985	650	3250	-	2176
52008	600	3000	-	510
52009	600	3000	-	540
52010	600	3000	-	570
52011	600	3000	-	600
52012	600	3000	-	630
52013	600	3000	-	660
52014	600	3000	-	690
52015	600	3000	-	720
52016	600	3000	-	750
52017	600	3000	-	780
52018	600	3000	-	810
52019	600	3000	-	840
52040	600	3000	-	1470
52041	600	3000	-	1500
52042	600	3000	-	1530
52043	600	3000	-	1560
52044	600	3000	-	1590
52045	600	3000	-	1620
52046	600	3000	-	1650
52047	600	3000	-	1680
52048	600	3000	-	1710
52049	600	3000	-	1740
52050	600	3000	-	1770
52051	600	3000	-	1800
52052	600	3000	-	1830
52053	600	3000	-	1860
52054	600	3000	-	1890
52055	600	3000	-	1920
52056	600	3000	-	1950
52057	600	3000	-	1980
52058	600	3000	-	2010
52059	600	3000	-	2040
52060	600	3000	-	2070
52061	600	3000	-	2100
52062	600	3000	-	2130
52063	600	3000	-	2160
52064	600	3000	-	2190
52065	600	3000	-	2220
52091	550	2750	-	513
52092	550	2750	-	540
52093	550	2750	-	567
52094	550	2750	-	594
52095	550	2750	-	621
52096	550	2750	-	648
52097	550	2750	-	675
52098	550	2750	-	702
52099	550	2750	-	729
52100	550	2750	-	756
52101	550	2750	-	783
52102	550	2750	-	810
52103	550	2750	-	837
52104	550	2750	-	864
52105	550	2750	-	891
52127	550	2750	-	1485
52128	550	2750	-	1512
52129	550	2750	-	1539
52130	550	2750	-	1566
52131	550	2750	-	1593
52132	550	2750	-	1620
52133	550	2750	-	1647
52134	550	2750	-	1674
52135	550	2750	-	1701
52136	550	2750	-	1728
52137	550	2750	-	1755
52138	550	2750	-	1782
52139	550	2750	-	1809
52140	550	2750	-	1836
52141	550	2750	-	1863
52142	550	2750	-	1890
52143	550	2750	-	1917
52144	550	2750	-	1944
52145	550	2750	-	1971
52146	550	2750	-	1998
52147	550	2750	-	2025
52148	550	2750	-	2052
52149	550	2750	-	2079
52150	550	2750	-	2106
52151	550	2750	-	2133
52152	550	2750	-	2160
52153	550	2750	-	2187
52154	550	2750	-	2214
52155	550	2750	-	2241
52156	550	2750	-	2268
52183	500	2500	-	500
52184	500	2500	-	525
52185	500	2500	-	550
52186	500	2500	-	575
52187	500	2500	-	600
52188	500	2500	-	625
52189	500	2500	-	650
52190	500	2500	-	675
52191	500	2500	-	700
52192	500	2500	-	725
52193	500	2500	-	750
52194	500	2500	-	775
52195	500	2500	-	800
52196	500	2500	-	825
52197	500	2500	-	850
52198	500	2500	-	875
52199	500	2500	-	900
52200	500	2500	-	925
52222	500	2500	-	1475
52223	500	2500	-	1500
52224	500	2500	-	1525
52225	500	2500	-	1550
52226	500	2500	-	1575
52227	500	2500	-	1600
52228	500	2500	-	1625
52229	500	2500	-	1650
52230	500	2500	-	1675
52231	500	2500	-	1700
52232	500	2500	-	1725
52233	500	2500	-	1750
52234	500	2500	-	1775
52235	500	2500	-	1800
52236	500	2500	-	1825
52237	500	2500	-	1850
52238	500	2500	-	1875
52239	500	2500	-	1900
52240	500	2500	-	1925
52241	500	2500	-	1950
52242	500	2500	-	1975
52243	500	2500	-	2000
52244	500	2500	-	2025
52245	500	2500	-	2050
52246	500	2500	-	2075
52247	500	2500	-	2100
52248	500	2500	-	2125
52249	500	2500	-	2150
52250	500	2500	-	2175
52251	500	2500	-	2200
52252	500	2500	-	2225
52253	500	2500	-	2250
52254	500	2500	-	2275
52255	500	2500	-	2300
52256	500	2500	-	2325
51622	950	4750	0.3	1457
52126	550	2750	0.4	1458
51540	1050	5250	0.4	1456
51579	1000	5000	1.0	1450
51942	650	3250	1.2	800
51365	1300	6500	1.4	520
51366	1300	6500	1.4	585
51367	1300	6500	1.4	650
51368	1300	6500	1.4	715
51369	1300	6500	1.4	780
51370	1300	6500	1.4	845
51371	1300	6500	1.4	910
51372	1300	6500	1.4	975
51373	1300	6500	1.4	1040
51374	1300	6500	1.4	1105
51375	1300	6500	1.4	1170
51376	1300	6500	1.4	1235
51377	1300	6500	1.4	1300
51378	1300	6500	1.4	1365
51379	1300	6500	1.4	1430
51392	1250	6250	1.4	496
51393	1250	6250	1.4	558
51394	1250	6250	1.4	620
51395	1250	6250	1.4	682
51396	1250	6250	1.4	744
51397	1250	6250	1.4	806
51398	1250	6250	1.4	868
51399	1250	6250	1.4	930
51400	1250	6250	1.4	992
51401	1250	6250	1.4	1054
51402	1250	6250	1.4	1116
51403	1250	6250	1.4	1178
51404	1250	6250	1.4	1240
51405	1250	6250	1.4	1302
51406	1250	6250	1.4	1364
51407	1250	6250	1.4	1426
51809	750	3750	1.5	703
51422	1200	6000	1.5	540
51423	1200	6000	1.5	600
51424	1200	6000	1.5	660
51425	1200	6000	1.5	720
51426	1200	6000	1.5	780
51427	1200	6000	1.5	840
51428	1200	6000	1.5	900
51429	1200	6000	1.5	960
51430	1200	6000	1.5	1020
51431	1200	6000	1.5	1080
51432	1200	6000	1.5	1140
51433	1200	6000	1.5	1200
51434	1200	6000	1.5	1260
51435	1200	6000	1.5	1320
51436	1200	6000	1.5	1380
51437	1200	6000	1.5	1440
51453	1150	5750	1.6	513
51454	1150	5750	1.6	570
51455	1150	5750	1.6	627
51456	1150	5750	1.6	684
51457	1150	5750	1.6	741
51458	1150	5750	1.6	798
51459	1150	5750	1.6	855
51460	1150	5750	1.6	912
51461	1150	5750	1.6	969
51462	1150	5750	1.6	1026
51463	1150	5750	1.6	1083
51464	1150	5750	1.6	1140
51465	1150	5750	1.6	1197
51466	1150	5750	1.6	1254
51467	1150	5750	1.6	1311
51468	1150	5750	1.6	1368
51469	1150	5750	1.6	1425
52201	500	2500	1.6	950
51486	1100	5500	1.6	495
51487	1100	5500	1.6	550
51488	1100	5500	1.6	605
51489	1100	5500	1.6	660
51490	1100	5500	1.6	715
51491	1100	5500	1.6	770
51492	1100	5500	1.6	825
51493	1100	5500	1.6	880
51494	1100	5500	1.6	935
51495	1100	5500	1.6	990
51496	1100	5500	1.6	1045
51497	1100	5500	1.6	1100
51498	1100	5500	1.6	1155
51499	1100	5500	1.6	1210
51500	1100	5500	1.6	1265
51501	1100	5500	1.6	1320
51502	1100	5500	1.6	1375
51503	1100	5500	1.6	1430
51522	1050	5250	1.7	520
51523	1050	5250	1.7	572
51524	1050	5250	1.7	624
51525	1050	5250	1.7	676
51526	1050	5250	1.7	728
51527	1050	5250	1.7	780
51528	1050	5250	1.7	832
51529	1050	5250	1.7	884
51530	1050	5250	1.7	936
51531	1050	5250	1.7	988
51532	1050	5250	1.7	1040
51533	1050	5250	1.7	1092
51534	1050	5250	1.7	1144
51535	1050	5250	1.7	1196
51536	1050	5250	1.7	1248
51537	1050	5250	1.7	1300
51538	1050	5250	1.7	1352
51539	1050	5250	1.7	1404
51560	1000	5000	1.8	500
51561	1000	5000	1.8	550
51562	1000	5000	1.8	600
51563	1000	5000	1.8	650
51564	1000	5000	1.8	700
51565	1000	5000	1.8	750
51566	1000	5000	1.8	800
51567	1000	5000	1.8	850
51568	1000	5000	1.8	900
51569	1000	5000	1.8	950
51570	1000	5000	1.8	1000
51571	1000	5000	1.8	1050
51572	1000	5000	1.8	1100
51573	1000	5000	1.8	1150
51574	1000	5000	1.8	1200
51575	1000	5000	1.8	1250
51576	1000	5000	1.8	1300
51577	1000	5000	1.8	1350
51578	1000	5000	1.8	1400
51602	950	4750	1.9	517
51603	950	4750	1.9	564
51604	950	4750	1.9	611
51605	950	4750	1.9	658
51606	950	4750	1.9	705
51607	950	4750	1.9	752
51608	950	4750	1.9	799
51609	950	4750	1.9	846
51610	950	4750	1.9	893
51611	950	4750	1.9	940
51612	950	4750	1.9	987
51613	950	4750	1.9	1034
51614	950	4750	1.9	1081
51615	950	4750	1.9	1128
51616	950	4750	1.9	1175
51617	950	4750	1.9	1222
51618	950	4750	1.9	1269
51619	950	4750	1.9	1316
51620	950	4750	1.9	1363
51621	950	4750	1.9	1410
51648	900	4500	2.0	585
51649	900	4500	2.0	630
51650	900	4500	2.0	675
51651	900	4500	2.0	720
51652	900	4500	2.0	765
51653	900	4500	2.0	810
51654	900	4500	2.0	855
51655	900	4500	2.0	900
51656	900	4500	2.0	945
51657	900	4500	2.0	990
51658	900	4500	2.0	1035
51659	900	4500	2.0	1080
51660	900	4500	2.0	1125
51661	900	4500	2.0	1170
51662	900	4500	2.0	1215
51663	900	4500	2.0	1260
51664	900	4500	2.0	1305
51665	900	4500	2.0	1350
51666	900	4500	2.0	1395
51667	900	4500	2.0	1440
52221	500	2500	2.0	1450
51697	850	4250	2.1	630
51698	850	4250	2.1	672
51699	850	4250	2.1	714
51700	850	4250	2.1	756
51701	850	4250	2.1	798
51702	850	4250	2.1	840
51703	850	4250	2.1	882
51704	850	4250	2.1	924
51705	850	4250	2.1	966
51706	850	4250	2.1	1008
51707	850	4250	2.1	1050
51708	850	4250	2.1	1092
51709	850	4250	2.1	1134
51710	850	4250	2.1	1176
51711	850	4250	2.1	1218
51712	850	4250	2.1	1260
51713	850	4250	2.1	1302
51714	850	4250	2.1	1344
51715	850	4250	2.1	1386
51716	850	4250	2.1	1428
51751	800	4000	2.2	680
51752	800	4000	2.2	720
51753	800	4000	2.2	760
51754	800	4000	2.2	800
51755	800	4000	2.2	840
51756	800	4000	2.2	880
51757	800	4000	2.2	920
51758	800	4000	2.2	960
51759	800	4000	2.2	1000
51760	800	4000	2.2	1040
51761	800	4000	2.2	1080
51762	800	4000	2.2	1120
51763	800	4000	2.2	1160
51764	800	4000	2.2	1200
51765	800	4000	2.2	1240
51766	800	4000	2.2	1280
51767	800	4000	2.2	1320
51768	800	4000	2.2	1360
51769	800	4000	2.2	1400
51770	800	4000	2.2	1440
51829	750	3750	2.3	1443
51810	750	3750	2.4	740
51811	750	3750	2.4	777
51812	750	3750	2.4	814
51813	750	3750	2.4	851
51814	750	3750	2.4	888
51815	750	3750	2.4	925
51816	750	3750	2.4	962
51817	750	3750	2.4	999
51818	750	3750	2.4	1036
51819	750	3750	2.4	1073
51820	750	3750	2.4	1110
51821	750	3750	2.4	1147
51822	750	3750	2.4	1184
51823	750	3750	2.4	1221
51824	750	3750	2.4	1258
51825	750	3750	2.4	1295
51826	750	3750	2.4	1332
51827	750	3750	2.4	1369
51828	750	3750	2.4	1406
51873	700	3500	2.6	770
51874	700	3500	2.6	805
51875	700	3500	2.6	840
51876	700	3500	2.6	875
51877	700	3500	2.6	910
51878	700	3500	2.6	945
51879	700	3500	2.6	980
51880	700	3500	2.6	1015
51881	700	3500	2.6	1050
51882	700	3500	2.6	1085
51883	700	3500	2.6	1120
51884	700	3500	2.6	1155
51885	700	3500	2.6	1190
51886	700	3500	2.6	1225
51887	700	3500	2.6	1260
51888	700	3500	2.6	1295
51889	700	3500	2.6	1330
51890	700	3500	2.6	1365
51891	700	3500	2.6	1400
51892	700	3500	2.6	1435
51943	650	3250	2.8	832
51944	650	3250	2.8	864
51945	650	3250	2.8	896
51946	650	3250	2.8	928
51947	650	3250	2.8	960
51948	650	3250	2.8	992
51949	650	3250	2.8	1024
51950	650	3250	2.8	1056
51951	650	3250	2.8	1088
51952	650	3250	2.8	1120
51953	650	3250	2.8	1152
51954	650	3250	2.8	1184
51955	650	3250	2.8	1216
51956	650	3250	2.8	1248
51957	650	3250	2.8	1280
51958	650	3250	2.8	1312
51959	650	3250	2.8	1344
51960	650	3250	2.8	1376
51961	650	3250	2.8	1408
51962	650	3250	2.8	1440
52020	600	3000	3.0	870
52021	600	3000	3.0	900
52022	600	3000	3.0	930
52023	600	3000	3.0	960
52024	600	3000	3.0	990
52025	600	3000	3.0	1020
52026	600	3000	3.0	1050
52027	600	3000	3.0	1080
52028	600	3000	3.0	1110
52029	600	3000	3.0	1140
52030	600	3000	3.0	1170
52031	600	3000	3.0	1200
52032	600	3000	3.0	1230
52033	600	3000	3.0	1260
52034	600	3000	3.0	1290
52035	600	3000	3.0	1320
52036	600	3000	3.0	1350
52037	600	3000	3.0	1380
52038	600	3000	3.0	1410
52039	600	3000	3.0	1440
52106	550	2750	3.3	918
52107	550	2750	3.3	945
52108	550	2750	3.3	972
52109	550	2750	3.3	999
52110	550	2750	3.3	1026
52111	550	2750	3.3	1053
52112	550	2750	3.3	1080
52113	550	2750	3.3	1107
52114	550	2750	3.3	1134
52115	550	2750	3.3	1161
52116	550	2750	3.3	1188
52117	550	2750	3.3	1215
52118	550	2750	3.3	1242
52119	550	2750	3.3	1269
52120	550	2750	3.3	1296
52121	550	2750	3.3	1323
52122	550	2750	3.3	1350
52123	550	2750	3.3	1377
52124	550	2750	3.3	1404
52125	550	2750	3.3	1431
52202	500	2500	3.6	975
52203	500	2500	3.6	1000
52204	500	2500	3.6	1025
52205	500	2500	3.6	1050
52206	500	2500	3.6	1075
52207	500	2500	3.6	1100
52208	500	2500	3.6	1125
52209	500	2500	3.6	1150
52210	500	2500	3.6	1175
52211	500	2500	3.6	1200
52212	500	2500	3.6	1225
52213	500	2500	3.6	1250
52214	500	2500	3.6	1275
52215	500	2500	3.6	1300
52216	500	2500	3.6	1325
52217	500	2500	3.6	1350
52218	500	2500	3.6	1375
52219	500	2500	3.6	1400
52220	500	2500	3.6	1425

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
51381	1300	43.0	6500	-	1560	43.0
51382	1300	43.0	6500	-	1625	43.0
51383	1300	43.0	6500	-	1690	43.0
51410	1250	41.4	6250	-	1612	41.4
51411	1250	41.4	6250	-	1674	41.4
51412	1250	41.4	6250	-	1736	41.4
51441	1200	39.7	6000	-	1680	39.7
51442	1200	39.7	6000	-	1740	39.7
51443	1200	39.7	6000	-	1800	39.7
51474	1150	38.1	5750	-	1710	38.1
51475	1150	38.1	5750	-	1767	38.1
51476	1150	38.1	5750	-	1824	38.1
51509	1100	36.4	5500	-	1760	36.4
51510	1100	36.4	5500	-	1815	36.4
51511	1100	36.4	5500	-	1870	36.4
51547	1050	34.8	5250	-	1820	34.8
51548	1050	34.8	5250	-	1872	34.8
51549	1050	34.8	5250	-	1924	34.8
51587	1000	33.1	5000	-	1850	33.1
51588	1000	33.1	5000	-	1900	33.1
51589	1000	33.1	5000	-	1950	33.1
51590	1000	33.1	5000	-	2000	33.1
51364	1300	34.8	6500	1.4	455	32.0
51632	950	31.5	4750	-	1927	31.5
51633	950	31.5	4750	-	1974	31.5
51634	950	31.5	4750	-	2021	31.5
51679	900	29.8	4500	-	1980	29.8
51680	900	29.8	4500	-	2025	29.8
51681	900	29.8	4500	-	2070	29.8
51730	850	28.1	4250	-	2016	28.1
51731	850	28.1	4250	-	2058	28.1
51732	850	28.1	4250	-	2100	28.1
51733	850	28.1	4250	-	2142	28.1
51452	1150	31.2	5750	1.6	456	28.1
51786	800	26.5	4000	-	2080	26.5
51787	800	26.5	4000	-	2120	26.5
51788	800	26.5	4000	-	2160	26.5
51789	800	26.5	4000	-	2200	26.5
51601	950	25.8	4750	-	470	25.8
51521	1050	28.5	5250	1.7	468	25.1
51847	750	24.8	3750	-	2109	24.8
51848	750	24.8	3750	-	2146	24.8
51849	750	24.8	3750	-	2183	24.8
51850	750	24.8	3750	-	2220	24.8
51913	700	23.2	3500	-	2170	23.2
51914	700	23.2	3500	-	2205	23.2
51915	700	23.2	3500	-	2240	23.2
51916	700	23.2	3500	-	2275	23.2
51693	850	23.1	4250	-	462	23.1
51559	1000	24.6	5000	0.8	450	23.0
51746	800	22.3	4000	-	480	22.3
51645	900	22.2	4500	-	450	22.2
51803	750	21.6	3750	-	481	21.6
51986	650	21.5	3250	-	2208	21.5
51987	650	21.5	3250	-	2240	21.5
51988	650	21.5	3250	-	2272	21.5
51989	650	21.5	3250	-	2304	21.5
51990	650	21.5	3250	-	2336	21.5
51485	1100	23.9	5500	1.6	440	20.6
52066	600	19.9	3000	-	2250	19.9
52067	600	19.9	3000	-	2280	19.9
52068	600	19.9	3000	-	2310	19.9
52069	600	19.9	3000	-	2340	19.9
52070	600	19.9	3000	-	2370	19.9
52071	600	19.9	3000	-	2400	19.9
51420	1200	21.7	6000	1.5	420	18.7
51864	700	18.7	3500	-	455	18.7
51357	1300	20.9	6500	1.2	0	18.4
51358	1300	20.9	6500	1.2	65	18.4
51359	1300	20.9	6500	1.2	130	18.4
51363	1300	21.0	6500	1.4	390	18.3
52157	550	18.2	2750	-	2295	18.2
52158	550	18.2	2750	-	2322	18.2
52159	550	18.2	2750	-	2349	18.2
52160	550	18.2	2750	-	2376	18.2
52161	550	18.2	2750	-	2403	18.2
52162	550	18.2	2750	-	2430	18.2
51932	650	18.2	3250	-	480	18.2
51600	950	17.8	4750	-	423	17.8
51385	1250	20.1	6250	1.3	62	17.5
51386	1250	20.1	6250	1.3	124	17.5
51387	1250	20.1	6250	1.3	186	17.5
51802	750	17.4	3750	-	444	17.4
51745	800	17.4	4000	-	440	17.4
51390	1250	20.1	6250	1.4	372	17.2
51483	1100	17.7	5500	0.3	330	17.1
51362	1300	20.9	6500	2.0	325	16.9
51519	1050	16.9	5250	-	364	16.9
52007	600	16.8	3000	-	480	16.8
51558	1000	16.7	5000	-	400	16.7
51417	1200	19.3	6000	1.3	240	16.6
52257	500	16.6	2500	-	2350	16.6
52258	500	16.6	2500	-	2375	16.6
52259	500	16.6	2500	-	2400	16.6
52260	500	16.6	2500	-	2425	16.6
52261	500	16.6	2500	-	2450	16.6
52262	500	16.6	2500	-	2475	16.6
52263	500	16.6	2500	-	2500	16.6
52090	550	16.5	2750	-	486	16.5
51419	1200	19.3	6000	1.5	360	16.3
51557	1000	16.1	5000	-	350	16.1
51451	1150	19.2	5750	1.6	399	16.0
51931	650	16.0	3250	-	448	16.0
51644	900	15.8	4500	-	405	15.8
51449	1150	18.5	5750	1.4	285	15.7
51360	1300	20.9	6500	2.6	195	15.7
51361	1300	20.9	6500	2.6	260	15.7
51692	850	15.4	4250	-	420	15.4
51450	1150	18.5	5750	1.6	342	15.4
51599	950	15.3	4750	-	376	15.3
51520	1050	18.6	5250	1.7	416	15.1
52089	550	14.9	2750	-	459	14.9
52006	600	14.8	3000	-	450	14.8
51388	1250	20.1	6250	2.7	248	14.7
51484	1100	17.9	5500	1.6	385	14.6
51643	900	14.5	4500	-	360	14.5
51598	950	15.3	4750	0.4	329	14.4
51690	850	13.7	4250	-	336	13.7
51691	850	13.7	4250	-	378	13.7
52182	500	13.6	2500	-	475	13.6
51744	800	13.3	4000	-	400	13.3
51801	750	13.3	3750	-	407	13.3
51743	800	12.9	4000	-	360	12.9
51863	700	12.7	3500	-	420	12.7
52181	500	12.3	2500	-	450	12.3
51799	750	12.1	3750	-	333	12.1
51800	750	12.1	3750	-	370	12.1
51930	650	11.5	3250	-	416	11.5
51862	700	11.4	3500	-	385	11.4
51861	700	11.3	3500	-	350	11.3
52088	550	11.1	2750	-	432	11.1
52005	600	10.9	3000	-	420	10.9
51929	650	10.6	3250	-	384	10.6
51928	650	10.5	3250	-	352	10.5

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
51421	1200	33.5	6000	1.5	480	30.5
51391	1250	25.3	6250	1.4	434	22.4
51384	1250	20.1	6250	1.3	0	17.5
51413	1200	19.3	6000	1.3	0	16.6
51414	1200	19.3	6000	1.3	60	16.6
51415	1200	19.3	6000	1.3	120	16.6
51416	1200	19.3	6000	1.3	180	16.6
51444	1150	18.5	5750	1.4	0	15.7
51445	1150	18.5	5750	1.4	57	15.7
51446	1150	18.5	5750	1.4	114	15.7
51447	1150	18.5	5750	1.4	171	15.7
51448	1150	18.5	5750	1.4	228	15.7
51477	1100	17.7	5500	1.5	0	14.8
51478	1100	17.7	5500	1.5	55	14.8
51479	1100	17.7	5500	1.5	110	14.8
51480	1100	17.7	5500	1.5	165	14.8
51481	1100	17.7	5500	1.5	220	14.8
51482	1100	17.7	5500	1.5	275	14.8
51389	1250	20.1	6250	2.7	310	14.7
51512	1050	16.9	5250	1.5	0	13.8
51513	1050	16.9	5250	1.5	52	13.8
51514	1050	16.9	5250	1.5	104	13.8
51515	1050	16.9	5250	1.5	156	13.8
51516	1050	16.9	5250	1.5	208	13.8
51517	1050	16.9	5250	1.5	260	13.8
51518	1050	16.9	5250	1.5	312	13.8
51418	1200	19.3	6000	2.8	300	13.6
51550	1000	16.1	5000	1.6	0	12.9
51551	1000	16.1	5000	1.6	50	12.9
51552	1000	16.1	5000	1.6	100	12.9
51553	1000	16.1	5000	1.6	150	12.9
51554	1000	16.1	5000	1.6	200	12.9
51555	1000	16.1	5000	1.6	250	12.9
51556	1000	16.1	5000	1.6	300	12.9
51591	950	15.3	4750	1.7	0	11.9
51592	950	15.3	4750	1.7	47	11.9
51593	950	15.3	4750	1.7	94	11.9
51594	950	15.3	4750	1.7	141	11.9
51595	950	15.3	4750	1.7	188	11.9
51596	950	15.3	4750	1.7	235	11.9
51597	950	15.3	4750	1.7	282	11.9
51635	900	14.5	4500	1.8	0	10.9
51636	900	14.5	4500	1.8	45	10.9
51637	900	14.5	4500	1.8	90	10.9
51638	900	14.5	4500	1.8	135	10.9
51639	900	14.5	4500	1.8	180	10.9
51640	900	14.5	4500	1.8	225	10.9
51641	900	14.5	4500	1.8	270	10.9
51642	900	14.5	4500	1.8	315	10.9
51682	850	13.7	4250	1.9	0	9.9
51683	850	13.7	4250	1.9	42	9.9
51684	850	13.7	4250	1.9	84	9.9
51685	850	13.7	4250	1.9	126	9.9
51686	850	13.7	4250	1.9	168	9.9
51687	850	13.7	4250	1.9	210	9.9
51688	850	13.7	4250	1.9	252	9.9
51689	850	13.7	4250	1.9	294	9.9
51742	800	12.9	4000	1.6	320	9.6
51734	800	12.9	4000	2.0	0	8.9
51735	800	12.9	4000	2.0	40	8.9
51736	800	12.9	4000	2.0	80	8.9
51737	800	12.9	4000	2.0	120	8.9
51738	800	12.9	4000	2.0	160	8.9
51739	800	12.9	4000	2.0	200	8.9
51740	800	12.9	4000	2.0	240	8.9
51741	800	12.9	4000	2.0	280	8.9
51790	750	12.1	3750	2.1	0	7.8
51791	750	12.1	3750	2.1	37	7.8
51792	750	12.1	3750	2.1	74	7.8
51793	750	12.1	3750	2.1	111	7.8
51794	750	12.1	3750	2.1	148	7.8
51795	750	12.1	3750	2.1	185	7.8
51796	750	12.1	3750	2.1	222	7.8
51797	750	12.1	3750	2.1	259	7.8
51798	750	12.1	3750	2.1	296	7.8
51851	700	11.3	3500	2.3	0	6.7
51852	700	11.3	3500	2.3	35	6.7
51853	700	11.3	3500	2.3	70	6.7
51854	700	11.3	3500	2.3	105	6.7
51855	700	11.3	3500	2.3	140	6.7
51856	700	11.3	3500	2.3	175	6.7
51857	700	11.3	3500	2.3	210	6.7
51858	700	11.3	3500	2.3	245	6.7
51859	700	11.3	3500	2.3	280	6.7
51860	700	11.3	3500	2.3	315	6.7
51927	650	10.5	3250	2.0	320	6.5
52163	500	12.2	2500	3.2	0	5.8
51917	650	10.5	3250	2.5	0	5.5
51918	650	10.5	3250	2.5	32	5.5
51919	650	10.5	3250	2.5	64	5.5
51920	650	10.5	3250	2.5	96	5.5
51921	650	10.5	3250	2.5	128	5.5
51922	650	10.5	3250	2.5	160	5.5
51923	650	10.5	3250	2.5	192	5.5
51924	650	10.5	3250	2.5	224	5.5
51925	650	10.5	3250	2.5	256	5.5
51926	650	10.5	3250	2.5	288	5.5

Overall score = score_ratio - 2 * repetitive DNA content