Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign67 - back to list

Summary
Image
Unique putative probes (330)
Putative probes (50)
Redundant probes (191)

Summary

`./analyse_probe_search_results : Fri Oct 7 10:51:08 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign67
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 1
  id            : 58
  strand        : 1
  -------------

Design window length: 6001bp

All jobs for probe design are successful - total: 108
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 2146 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 2146/2146
Putative probes that are unique                 : 330/2146
Putative probes exceeding selection criteria    : 241/2146 (but not unique)
Putative probes with successfully-picked primers: 419/571

Identified 191 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
55446	1300	6500	-	650
55447	1300	6500	-	715
55448	1300	6500	-	780
55449	1300	6500	-	845
55520	1250	6250	-	682
55521	1250	6250	-	744
55522	1250	6250	-	806
55523	1250	6250	-	868
55524	1250	6250	-	930
55597	1200	6000	-	660
55598	1200	6000	-	720
55599	1200	6000	-	780
55600	1200	6000	-	840
55601	1200	6000	-	900
55602	1200	6000	-	960
55679	1150	5750	-	684
55680	1150	5750	-	741
55681	1150	5750	-	798
55682	1150	5750	-	855
55683	1150	5750	-	912
55684	1150	5750	-	969
55685	1150	5750	-	1026
55765	1100	5500	-	660
55766	1100	5500	-	715
55767	1100	5500	-	770
55768	1100	5500	-	825
55769	1100	5500	-	880
55770	1100	5500	-	935
55771	1100	5500	-	990
55772	1100	5500	-	1045
55773	1100	5500	-	1100
55856	1050	5250	-	676
55857	1050	5250	-	728
55858	1050	5250	-	780
55859	1050	5250	-	832
55860	1050	5250	-	884
55861	1050	5250	-	936
55862	1050	5250	-	988
55863	1050	5250	-	1040
55864	1050	5250	-	1092
55865	1050	5250	-	1144
55952	1000	5000	-	650
55953	1000	5000	-	700
55954	1000	5000	-	750
55955	1000	5000	-	800
55956	1000	5000	-	850
55957	1000	5000	-	900
55958	1000	5000	-	950
55959	1000	5000	-	1000
55960	1000	5000	-	1050
55961	1000	5000	-	1100
55962	1000	5000	-	1150
55963	1000	5000	-	1200
56054	950	4750	-	658
56055	950	4750	-	705
56056	950	4750	-	752
56057	950	4750	-	799
56058	950	4750	-	846
56059	950	4750	-	893
56060	950	4750	-	940
56061	950	4750	-	987
56062	950	4750	-	1034
56063	950	4750	-	1081
56064	950	4750	-	1128
56065	950	4750	-	1175
56066	950	4750	-	1222
56163	900	4500	-	675
56164	900	4500	-	720
56165	900	4500	-	765
56166	900	4500	-	810
56167	900	4500	-	855
56168	900	4500	-	900
56169	900	4500	-	945
56170	900	4500	-	990
56171	900	4500	-	1035
56172	900	4500	-	1080
56173	900	4500	-	1125
56174	900	4500	-	1170
56175	900	4500	-	1215
56176	900	4500	-	1260
56278	850	4250	-	672
56279	850	4250	-	714
56280	850	4250	-	756
56281	850	4250	-	798
56282	850	4250	-	840
56283	850	4250	-	882
56284	850	4250	-	924
56285	850	4250	-	966
56286	850	4250	-	1008
56287	850	4250	-	1050
56288	850	4250	-	1092
56289	850	4250	-	1134
56290	850	4250	-	1176
56291	850	4250	-	1218
56292	850	4250	-	1260
56293	850	4250	-	1302
56294	850	4250	-	1344
56402	800	4000	-	680
56403	800	4000	-	720
56404	800	4000	-	760
56405	800	4000	-	800
56406	800	4000	-	840
56407	800	4000	-	880
56408	800	4000	-	920
56409	800	4000	-	960
56410	800	4000	-	1000
56411	800	4000	-	1040
56412	800	4000	-	1080
56413	800	4000	-	1120
56414	800	4000	-	1160
56415	800	4000	-	1200
56416	800	4000	-	1240
56417	800	4000	-	1280
56418	800	4000	-	1320
56419	800	4000	-	1360
56420	800	4000	-	1400
56534	750	3750	-	666
56535	750	3750	-	703
56536	750	3750	-	740
56537	750	3750	-	777
56538	750	3750	-	814
56539	750	3750	-	851
56540	750	3750	-	888
56541	750	3750	-	925
56542	750	3750	-	962
56543	750	3750	-	999
56544	750	3750	-	1036
56545	750	3750	-	1073
56546	750	3750	-	1110
56547	750	3750	-	1147
56548	750	3750	-	1184
56549	750	3750	-	1221
56550	750	3750	-	1258
56551	750	3750	-	1295
56552	750	3750	-	1332
56553	750	3750	-	1369
56554	750	3750	-	1406
56555	750	3750	-	1443
56677	700	3500	-	665
56678	700	3500	-	700
56679	700	3500	-	735
56680	700	3500	-	770
56681	700	3500	-	805
56682	700	3500	-	840
56683	700	3500	-	875
56684	700	3500	-	910
56685	700	3500	-	945
56686	700	3500	-	980
56687	700	3500	-	1015
56688	700	3500	-	1050
56689	700	3500	-	1085
56690	700	3500	-	1120
56691	700	3500	-	1155
56692	700	3500	-	1190
56693	700	3500	-	1225
56694	700	3500	-	1260
56695	700	3500	-	1295
56696	700	3500	-	1330
56697	700	3500	-	1365
56698	700	3500	-	1400
56699	700	3500	-	1435
56700	700	3500	-	1470
56831	650	3250	-	672
56832	650	3250	-	704
56833	650	3250	-	736
56834	650	3250	-	768
56835	650	3250	-	800
56836	650	3250	-	832
56837	650	3250	-	864
56838	650	3250	-	896
56839	650	3250	-	928
56840	650	3250	-	960
56841	650	3250	-	992
56842	650	3250	-	1024
56843	650	3250	-	1056
56844	650	3250	-	1088
56845	650	3250	-	1120
56846	650	3250	-	1152
56847	650	3250	-	1184
56848	650	3250	-	1216
56849	650	3250	-	1248
56850	650	3250	-	1280
56851	650	3250	-	1312
56852	650	3250	-	1344
56853	650	3250	-	1376
56854	650	3250	-	1408
56855	650	3250	-	1440
56856	650	3250	-	1472
56857	650	3250	-	1504
56858	650	3250	-	1536
57000	600	3000	-	660
57001	600	3000	-	690
57002	600	3000	-	720
57003	600	3000	-	750
57004	600	3000	-	780
57005	600	3000	-	810
57006	600	3000	-	840
57007	600	3000	-	870
57008	600	3000	-	900
57009	600	3000	-	930
57010	600	3000	-	960
57011	600	3000	-	990
57012	600	3000	-	1020
57013	600	3000	-	1050
57014	600	3000	-	1080
57015	600	3000	-	1110
57016	600	3000	-	1140
57017	600	3000	-	1170
57018	600	3000	-	1200
57019	600	3000	-	1230
57020	600	3000	-	1260
57021	600	3000	-	1290
57022	600	3000	-	1320
57023	600	3000	-	1350
57024	600	3000	-	1380
57025	600	3000	-	1410
57026	600	3000	-	1440
57027	600	3000	-	1470
57028	600	3000	-	1500
57029	600	3000	-	1530
57030	600	3000	-	1560
57031	600	3000	-	1590
57183	550	2750	-	648
57184	550	2750	-	675
57185	550	2750	-	702
57186	550	2750	-	729
57187	550	2750	-	756
57188	550	2750	-	783
57189	550	2750	-	810
57190	550	2750	-	837
57191	550	2750	-	864
57192	550	2750	-	891
57193	550	2750	-	918
57194	550	2750	-	945
57195	550	2750	-	972
57196	550	2750	-	999
57197	550	2750	-	1026
57198	550	2750	-	1053
57199	550	2750	-	1080
57200	550	2750	-	1107
57201	550	2750	-	1134
57202	550	2750	-	1161
57203	550	2750	-	1188
57204	550	2750	-	1215
57205	550	2750	-	1242
57206	550	2750	-	1269
57207	550	2750	-	1296
57208	550	2750	-	1323
57209	550	2750	-	1350
57210	550	2750	-	1377
57211	550	2750	-	1404
57212	550	2750	-	1431
57213	550	2750	-	1458
57214	550	2750	-	1485
57215	550	2750	-	1512
57216	550	2750	-	1539
57217	550	2750	-	1566
57218	550	2750	-	1593
57219	550	2750	-	1620
57220	550	2750	-	1647
57387	500	2500	-	650
57388	500	2500	-	675
57389	500	2500	-	700
57390	500	2500	-	725
57391	500	2500	-	750
57392	500	2500	-	775
57393	500	2500	-	800
57394	500	2500	-	825
57395	500	2500	-	850
57396	500	2500	-	875
57397	500	2500	-	900
57398	500	2500	-	925
57399	500	2500	-	950
57400	500	2500	-	975
57401	500	2500	-	1000
57402	500	2500	-	1025
57403	500	2500	-	1050
57404	500	2500	-	1075
57405	500	2500	-	1100
57406	500	2500	-	1125
57407	500	2500	-	1150
57408	500	2500	-	1175
57409	500	2500	-	1200
57410	500	2500	-	1225
57411	500	2500	-	1250
57412	500	2500	-	1275
57413	500	2500	-	1300
57414	500	2500	-	1325
57415	500	2500	-	1350
57416	500	2500	-	1375
57417	500	2500	-	1400
57418	500	2500	-	1425
57419	500	2500	-	1450
57420	500	2500	-	1475
57421	500	2500	-	1500
57422	500	2500	-	1525
57423	500	2500	-	1550
57424	500	2500	-	1575
57425	500	2500	-	1600
57426	500	2500	-	1625
57427	500	2500	-	1650
57428	500	2500	-	1675
57429	500	2500	-	1700
56401	800	4000	0.2	640
56830	650	3250	0.3	640
56177	900	4500	0.6	1305
56701	700	3500	0.7	1505
55450	1300	6500	0.8	910
55678	1150	5750	1.3	627
56162	900	4500	1.3	630
56277	850	4250	1.4	630
55519	1250	6250	1.4	620
55764	1100	5500	1.6	605
55603	1200	6000	1.7	1020
55855	1050	5250	1.7	624
56676	700	3500	1.7	630
56533	750	3750	1.7	629
56053	950	4750	1.9	611
56067	950	4750	2.0	1269
56999	600	3000	2.0	630
56829	650	3250	2.8	608
56859	650	3250	2.8	1568
57182	550	2750	3.3	621
57032	600	3000	3.3	1620
57386	500	2500	3.4	625
56556	750	3750	4.0	1480
57221	550	2750	4.4	1674
57430	500	2500	5.0	1725
57461	500	2500	9.8	2500
57462	500	2500	9.8	2525

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
55444	1300	41.4	6500	1.4	520	38.6
55445	1300	41.4	6500	1.4	585	38.6
55517	1250	39.8	6250	1.4	496	36.9
55595	1200	38.2	6000	1.5	540	35.2
55676	1150	36.6	5750	1.6	513	33.5
55677	1150	36.6	5750	1.6	570	33.5
55436	1300	37.8	6500	2.2	0	33.5
55437	1300	37.8	6500	2.2	65	33.5
55438	1300	37.8	6500	2.2	130	33.5
55439	1300	37.8	6500	2.2	195	33.5
55440	1300	37.8	6500	2.2	260	33.5
55441	1300	37.8	6500	2.2	325	33.5
55442	1300	37.8	6500	2.2	390	33.5
55443	1300	37.8	6500	2.2	455	33.5
55686	1150	38.3	5750	2.9	1083	32.6
55510	1250	36.3	6250	2.2	62	31.9
55511	1250	36.3	6250	2.2	124	31.9
55512	1250	36.3	6250	2.2	186	31.9
55513	1250	36.3	6250	2.2	248	31.9
55763	1100	35.0	5500	1.6	550	31.8
55762	1100	34.4	5500	1.6	495	31.1
55853	1050	33.4	5250	1.7	520	30.0
55854	1050	33.4	5250	1.7	572	30.0
55525	1250	36.3	6250	3.4	992	29.6
55951	1000	32.7	5000	1.8	600	29.1
55949	1000	31.8	5000	1.8	500	28.2
55950	1000	31.8	5000	1.8	550	28.2
56051	950	30.3	4750	1.9	517	26.5
56052	950	30.3	4750	1.9	564	26.5
56160	900	28.7	4500	2.0	540	24.7
56161	900	28.7	4500	2.0	585	24.7
56159	900	28.1	4500	2.0	495	24.1
56274	850	27.1	4250	2.1	504	22.8
56275	850	27.1	4250	2.1	546	22.8
56276	850	27.1	4250	2.1	588	22.8
55866	1050	29.3	5250	4.4	1196	20.6
55774	1100	28.4	5500	5.0	1155	18.4
55964	1000	25.8	5000	5.0	1250	15.8
57159	550	18.0	2750	1.8	0	14.3
56978	600	17.4	3000	1.7	0	14.1
57361	500	16.6	2500	2.0	0	12.6
57362	500	16.6	2500	2.0	25	12.6
57160	550	16.0	2750	1.8	27	12.4
57161	550	16.0	2750	1.8	54	12.4
57363	500	16.3	2500	2.0	50	12.3
57578	500	12.3	2500	0.4	5425	11.5
57364	500	14.5	2500	2.0	75	10.5
57365	500	14.5	2500	2.0	100	10.5
57577	500	14.6	2500	2.2	5400	10.2
57547	500	12.1	2500	3.4	4650	5.3

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
55518	1250	39.8	6250	1.4	558	36.9
55596	1200	39.2	6000	1.5	600	36.2
55509	1250	36.3	6250	2.2	0	31.9
55514	1250	36.3	6250	2.2	310	31.9
55515	1250	36.3	6250	2.2	372	31.9
55516	1250	36.3	6250	2.2	434	31.9
55594	1200	34.9	6000	2.2	480	30.6
55586	1200	34.9	6000	2.3	0	30.2
55587	1200	34.9	6000	2.3	60	30.2
55588	1200	34.9	6000	2.3	120	30.2
55589	1200	34.9	6000	2.3	180	30.2
55590	1200	34.9	6000	2.3	240	30.2
55591	1200	34.9	6000	2.3	300	30.2
55592	1200	34.9	6000	2.3	360	30.2
55593	1200	34.9	6000	2.3	420	30.2
55667	1150	33.4	5750	2.4	0	28.6
55668	1150	33.4	5750	2.4	57	28.6
55669	1150	33.4	5750	2.4	114	28.6
55670	1150	33.4	5750	2.4	171	28.6
55671	1150	33.4	5750	2.4	228	28.6
55672	1150	33.4	5750	2.4	285	28.6
55673	1150	33.4	5750	2.4	342	28.6
55674	1150	33.4	5750	2.4	399	28.6
55675	1150	33.4	5750	2.4	456	28.6
55753	1100	32.0	5500	2.5	0	26.9
55754	1100	32.0	5500	2.5	55	26.9
55755	1100	32.0	5500	2.5	110	26.9
55756	1100	32.0	5500	2.5	165	26.9
55757	1100	32.0	5500	2.5	220	26.9
55758	1100	32.0	5500	2.5	275	26.9
55759	1100	32.0	5500	2.5	330	26.9
55760	1100	32.0	5500	2.5	385	26.9
55761	1100	32.0	5500	2.5	440	26.9
55843	1050	30.5	5250	2.7	0	25.2
55844	1050	30.5	5250	2.7	52	25.2
55845	1050	30.5	5250	2.7	104	25.2
55846	1050	30.5	5250	2.7	156	25.2
55847	1050	30.5	5250	2.7	208	25.2
55848	1050	30.5	5250	2.7	260	25.2
55849	1050	30.5	5250	2.7	312	25.2
55850	1050	30.5	5250	2.7	364	25.2
55851	1050	30.5	5250	2.7	416	25.2
55852	1050	30.5	5250	2.7	468	25.2
55939	1000	29.1	5000	2.8	0	23.5
55940	1000	29.1	5000	2.8	50	23.5
55941	1000	29.1	5000	2.8	100	23.5
55942	1000	29.1	5000	2.8	150	23.5
55943	1000	29.1	5000	2.8	200	23.5
55944	1000	29.1	5000	2.8	250	23.5
55945	1000	29.1	5000	2.8	300	23.5
55946	1000	29.1	5000	2.8	350	23.5
55947	1000	29.1	5000	2.8	400	23.5
55948	1000	29.1	5000	2.8	450	23.5
56040	950	27.6	4750	2.9	0	21.7
56041	950	27.6	4750	2.9	47	21.7
56042	950	27.6	4750	2.9	94	21.7
56043	950	27.6	4750	2.9	141	21.7
56044	950	27.6	4750	2.9	188	21.7
56045	950	27.6	4750	2.9	235	21.7
56046	950	27.6	4750	2.9	282	21.7
56047	950	27.6	4750	2.9	329	21.7
56048	950	27.6	4750	2.9	376	21.7
56049	950	27.6	4750	2.9	423	21.7
56050	950	27.6	4750	2.9	470	21.7
56400	800	26.1	4000	2.2	600	21.6
56398	800	25.5	4000	2.2	520	21.0
56399	800	25.5	4000	2.2	560	21.0
56148	900	26.2	4500	3.1	0	19.9
56149	900	26.2	4500	3.1	45	19.9
56150	900	26.2	4500	3.1	90	19.9
56151	900	26.2	4500	3.1	135	19.9
56152	900	26.2	4500	3.1	180	19.9
56153	900	26.2	4500	3.1	225	19.9
56154	900	26.2	4500	3.1	270	19.9
56155	900	26.2	4500	3.1	315	19.9
56156	900	26.2	4500	3.1	360	19.9
56157	900	26.2	4500	3.1	405	19.9
56158	900	26.2	4500	3.1	450	19.9
56295	850	28.3	4250	4.2	1386	19.9
56530	750	23.9	3750	2.4	518	19.1
56531	750	23.9	3750	2.4	555	19.1
56532	750	23.9	3750	2.4	592	19.1
56262	850	24.7	4250	3.3	0	18.1
56263	850	24.7	4250	3.3	42	18.1
56264	850	24.7	4250	3.3	84	18.1
56265	850	24.7	4250	3.3	126	18.1
56266	850	24.7	4250	3.3	168	18.1
56267	850	24.7	4250	3.3	210	18.1
56268	850	24.7	4250	3.3	252	18.1
56269	850	24.7	4250	3.3	294	18.1
56270	850	24.7	4250	3.3	336	18.1
56271	850	24.7	4250	3.3	378	18.1
56272	850	24.7	4250	3.3	420	18.1
56273	850	24.7	4250	3.3	462	18.1
56675	700	22.4	3500	2.6	595	17.3
56673	700	22.3	3500	2.6	525	17.2
56674	700	22.3	3500	2.6	560	17.2
56397	800	23.3	4000	3.2	480	16.8
56385	800	23.3	4000	3.5	0	16.3
56386	800	23.3	4000	3.5	40	16.3
56387	800	23.3	4000	3.5	80	16.3
56388	800	23.3	4000	3.5	120	16.3
56389	800	23.3	4000	3.5	160	16.3
56390	800	23.3	4000	3.5	200	16.3
56391	800	23.3	4000	3.5	240	16.3
56392	800	23.3	4000	3.5	280	16.3
56393	800	23.3	4000	3.5	320	16.3
56394	800	23.3	4000	3.5	360	16.3
56395	800	23.3	4000	3.5	400	16.3
56396	800	23.3	4000	3.5	440	16.3
56672	700	20.5	3500	2.6	490	15.3
56826	650	20.7	3250	2.8	512	15.2
56827	650	20.7	3250	2.8	544	15.2
56828	650	20.7	3250	2.8	576	15.2
56529	750	21.8	3750	3.3	481	15.1
56516	750	21.8	3750	3.7	0	14.3
56517	750	21.8	3750	3.7	37	14.3
56518	750	21.8	3750	3.7	74	14.3
56519	750	21.8	3750	3.7	111	14.3
56520	750	21.8	3750	3.7	148	14.3
56521	750	21.8	3750	3.7	185	14.3
56522	750	21.8	3750	3.7	222	14.3
56523	750	21.8	3750	3.7	259	14.3
56524	750	21.8	3750	3.7	296	14.3
56525	750	21.8	3750	3.7	333	14.3
56526	750	21.8	3750	3.7	370	14.3
56527	750	21.8	3750	3.7	407	14.3
56528	750	21.8	3750	3.7	444	14.3
56421	800	24.0	4000	5.0	1440	14.0
56998	600	19.6	3000	3.0	600	13.6
56995	600	19.1	3000	3.0	510	13.1
56996	600	19.1	3000	3.0	540	13.1
56997	600	19.1	3000	3.0	570	13.1
56658	700	20.3	3500	4.0	0	12.3
56659	700	20.3	3500	4.0	35	12.3
56660	700	20.3	3500	4.0	70	12.3
56661	700	20.3	3500	4.0	105	12.3
56662	700	20.3	3500	4.0	140	12.3
56663	700	20.3	3500	4.0	175	12.3
56664	700	20.3	3500	4.0	210	12.3
56665	700	20.3	3500	4.0	245	12.3
56666	700	20.3	3500	4.0	280	12.3
56667	700	20.3	3500	4.0	315	12.3
56668	700	20.3	3500	4.0	350	12.3
56669	700	20.3	3500	4.0	385	12.3
56670	700	20.3	3500	4.0	420	12.3
56671	700	20.3	3500	4.0	455	12.3
56979	600	17.4	3000	2.7	30	12.1
57178	550	17.5	2750	3.3	513	11.0
57179	550	17.5	2750	3.3	540	11.0
57180	550	17.5	2750	3.3	567	11.0
57181	550	17.5	2750	3.3	594	11.0
56825	650	18.9	3250	4.0	480	10.9
56810	650	18.9	3250	4.3	0	10.3
56811	650	18.9	3250	4.3	32	10.3
56812	650	18.9	3250	4.3	64	10.3
56813	650	18.9	3250	4.3	96	10.3
56814	650	18.9	3250	4.3	128	10.3
56815	650	18.9	3250	4.3	160	10.3
56816	650	18.9	3250	4.3	192	10.3
56817	650	18.9	3250	4.3	224	10.3
56818	650	18.9	3250	4.3	256	10.3
56819	650	18.9	3250	4.3	288	10.3
56820	650	18.9	3250	4.3	320	10.3
56821	650	18.9	3250	4.3	352	10.3
56822	650	18.9	3250	4.3	384	10.3
56823	650	18.9	3250	4.3	416	10.3
56824	650	18.9	3250	4.3	448	10.3
57366	500	14.5	2500	2.2	125	10.1
57162	550	16.0	2750	3.1	81	9.8
57385	500	16.3	2500	3.6	600	9.1
56994	600	17.4	3000	4.3	480	8.8
57381	500	15.9	2500	3.6	500	8.7
57382	500	15.9	2500	3.6	525	8.7
57383	500	15.9	2500	3.6	550	8.7
57384	500	15.9	2500	3.6	575	8.7
57177	550	16.0	2750	3.6	486	8.7
56980	600	17.4	3000	4.7	60	8.1
56981	600	17.4	3000	4.7	90	8.1
56982	600	17.4	3000	4.7	120	8.1
56983	600	17.4	3000	4.7	150	8.1
56984	600	17.4	3000	4.7	180	8.1
56985	600	17.4	3000	4.7	210	8.1
56986	600	17.4	3000	4.7	240	8.1
56987	600	17.4	3000	4.7	270	8.1
56988	600	17.4	3000	4.7	300	8.1
56989	600	17.4	3000	4.7	330	8.1
56990	600	17.4	3000	4.7	360	8.1
56991	600	17.4	3000	4.7	390	8.1
56992	600	17.4	3000	4.7	420	8.1
56993	600	17.4	3000	4.7	450	8.1

Overall score = score_ratio - 2 * repetitive DNA content