Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign77 - back to list

Summary
Image
Unique putative probes (117)
Putative probes (232)
Redundant probes (231)

Summary

`./analyse_probe_search_results : Tue Oct 18 14:44:26 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign77
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : X
  id            : 62
  strand        : 1
  -------------

Design window length: 5001bp

All jobs for probe design are successful - total: 87
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 1729 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1729/1729
Putative probes that are unique                 : 117/1729
Putative probes exceeding selection criteria    : 463/1729 (but not unique)
Putative probes with successfully-picked primers: 580/580

Identified 231 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
60612	800	4000	-	3520
60669	750	3750	-	1443
60725	750	3750	-	3515
60726	750	3750	-	3552
60727	750	3750	-	3589
60787	700	3500	-	1470
60846	700	3500	-	3535
60847	700	3500	-	3570
60848	700	3500	-	3605
60849	700	3500	-	3640
60914	650	3250	-	1472
60915	650	3250	-	1504
60916	650	3250	-	1536
60978	650	3250	-	3520
60979	650	3250	-	3552
60980	650	3250	-	3584
60981	650	3250	-	3616
60982	650	3250	-	3648
60983	650	3250	-	3680
61053	600	3000	-	1470
61054	600	3000	-	1500
61055	600	3000	-	1530
61056	600	3000	-	1560
61057	600	3000	-	1590
61122	600	3000	-	3540
61123	600	3000	-	3570
61124	600	3000	-	3600
61125	600	3000	-	3630
61126	600	3000	-	3660
61127	600	3000	-	3690
61128	600	3000	-	3720
61129	600	3000	-	3750
61154	550	2750	-	81
61155	550	2750	-	108
61205	550	2750	-	1458
61206	550	2750	-	1485
61207	550	2750	-	1512
61208	550	2750	-	1539
61209	550	2750	-	1566
61210	550	2750	-	1593
61211	550	2750	-	1620
61282	550	2750	-	3537
61283	550	2750	-	3564
61284	550	2750	-	3591
61285	550	2750	-	3618
61286	550	2750	-	3645
61287	550	2750	-	3672
61288	550	2750	-	3699
61289	550	2750	-	3726
61290	550	2750	-	3753
61291	550	2750	-	3780
61292	550	2750	-	3807
61319	500	2500	-	75
61320	500	2500	-	100
61321	500	2500	-	125
61322	500	2500	-	150
61323	500	2500	-	175
61374	500	2500	-	1450
61375	500	2500	-	1475
61376	500	2500	-	1500
61377	500	2500	-	1525
61378	500	2500	-	1550
61379	500	2500	-	1575
61380	500	2500	-	1600
61381	500	2500	-	1625
61382	500	2500	-	1650
61383	500	2500	-	1675
61457	500	2500	-	3525
61458	500	2500	-	3550
61459	500	2500	-	3575
61460	500	2500	-	3600
61461	500	2500	-	3625
61462	500	2500	-	3650
61463	500	2500	-	3675
61464	500	2500	-	3700
61465	500	2500	-	3725
61466	500	2500	-	3750
61467	500	2500	-	3775
61468	500	2500	-	3800
61469	500	2500	-	3825
61470	500	2500	-	3850
60913	650	3250	0.2	1440
61052	600	3000	0.2	1440
61212	550	2750	0.5	1647
61156	550	2750	0.7	135
60786	700	3500	0.9	1435
61384	500	2500	1.2	1700
61007	600	3000	1.5	90
60788	700	3500	1.6	1505
61204	550	2750	1.8	1431
61373	500	2500	3.2	1425
60917	650	3250	3.7	1568
61324	500	2500	3.8	200
61058	600	3000	4.3	1620
60670	750	3750	4.8	1480
61213	550	2750	5.5	1674
60560	800	4000	5.9	1440
61385	500	2500	6.2	1725
60789	700	3500	6.6	1540
60918	650	3250	8.6	1600
61059	600	3000	9.3	1650
60671	750	3750	9.7	1517
61214	550	2750	10.4	1701
60561	800	4000	10.8	1480
61386	500	2500	11.2	1750
60459	850	4250	11.4	1428
60790	700	3500	11.6	1575
60919	650	3250	13.5	1632
61060	600	3000	14.3	1680
60672	750	3750	14.7	1554
61215	550	2750	15.3	1728
61387	500	2500	16.2	1775
60791	700	3500	16.6	1610
60920	650	3250	18.5	1664
61061	600	3000	19.3	1710
61216	550	2750	20.2	1755
61388	500	2500	21.2	1800

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
59814	1300	35.1	6500	1.1	2990	33.0
59815	1300	35.1	6500	1.1	3055	33.0
59816	1300	34.8	6500	1.1	3120	32.6
59817	1300	34.8	6500	1.1	3185	32.6
59941	1200	32.1	6000	-	3300	32.1
59807	1300	35.1	6500	1.7	2535	31.8
59808	1300	35.1	6500	1.7	2600	31.8
59809	1300	35.1	6500	1.7	2665	31.8
59810	1300	35.1	6500	1.7	2730	31.8
59811	1300	35.1	6500	1.7	2795	31.8
59812	1300	35.1	6500	1.7	2860	31.8
59813	1300	35.1	6500	1.7	2925	31.8
59873	1250	33.8	6250	1.1	2976	31.5
60008	1150	30.7	5750	-	3306	30.7
59869	1250	33.8	6250	1.8	2728	30.3
59870	1250	33.8	6250	1.8	2790	30.3
59871	1250	33.8	6250	1.8	2852	30.3
59872	1250	33.8	6250	1.8	2914	30.3
59936	1200	32.4	6000	1.2	3000	30.1
60009	1150	30.7	5750	0.5	3363	29.7
60078	1100	29.4	5500	-	3300	29.4
60079	1100	29.4	5500	-	3355	29.4
59935	1200	32.4	6000	1.7	2940	29.1
60080	1100	29.4	5500	0.3	3410	28.9
59878	1250	33.4	6250	2.3	3286	28.8
60002	1150	31.1	5750	1.2	2964	28.6
60003	1150	31.1	5750	1.2	3021	28.6
60152	1050	29.5	5250	0.6	3276	28.4
60231	1000	28.1	5000	-	3300	28.1
60232	1000	28.1	5000	-	3350	28.1
60153	1050	28.1	5250	-	3328	28.1
60154	1050	28.1	5250	-	3380	28.1
60155	1050	28.1	5250	-	3432	28.1
60072	1100	29.7	5500	1.3	2970	27.2
60073	1100	29.7	5500	1.3	3025	27.2
60074	1100	29.7	5500	1.3	3080	27.2
60233	1000	26.7	5000	-	3400	26.7
60234	1000	26.7	5000	-	3450	26.7
60235	1000	26.7	5000	-	3500	26.7
60316	950	26.7	4750	-	3290	26.7
60317	950	26.7	4750	-	3337	26.7
60318	950	26.7	4750	-	3384	26.7
59928	1200	32.4	6000	3.0	2520	26.4
60064	1100	30.9	5500	2.4	2530	26.2
60146	1050	28.4	5250	1.3	2964	25.7
60147	1050	28.4	5250	1.3	3016	25.7
60148	1050	28.4	5250	1.3	3068	25.7
60149	1050	28.4	5250	1.3	3120	25.7
60150	1050	28.4	5250	1.3	3172	25.7
59818	1300	34.2	6500	4.4	3250	25.4
60319	950	25.4	4750	-	3431	25.4
60320	950	25.4	4750	-	3478	25.4
60321	950	25.4	4750	-	3525	25.4
60406	900	25.3	4500	-	3285	25.3
60407	900	25.3	4500	-	3330	25.3
60408	900	25.3	4500	-	3375	25.3
60409	900	25.3	4500	-	3420	25.3
60410	900	25.3	4500	-	3465	25.3
60613	800	24.7	4000	-	3560	24.7
60224	1000	27.0	5000	1.4	2950	24.2
60225	1000	27.0	5000	1.4	3000	24.2
60226	1000	27.0	5000	1.4	3050	24.2
60227	1000	27.0	5000	1.4	3100	24.2
60228	1000	27.0	5000	1.4	3150	24.2
60229	1000	27.0	5000	1.4	3200	24.2
60411	900	24.1	4500	-	3510	24.1
60412	900	24.1	4500	-	3555	24.1
60413	900	24.1	4500	-	3600	24.1
60504	850	23.9	4250	-	3318	23.9
60505	850	23.9	4250	-	3360	23.9
60506	850	23.9	4250	-	3402	23.9
60507	850	23.9	4250	-	3444	23.9
60508	850	23.9	4250	-	3486	23.9
60156	1050	28.1	5250	2.6	3484	22.9
59994	1150	31.1	5750	4.2	2508	22.7
60309	950	25.7	4750	1.5	2961	22.7
60310	950	25.7	4750	1.5	3008	22.7
60311	950	25.7	4750	1.5	3055	22.7
60312	950	25.7	4750	1.5	3102	22.7
60313	950	25.7	4750	1.5	3149	22.7
60314	950	25.7	4750	1.5	3196	22.7
60315	950	25.7	4750	1.5	3243	22.7
60509	850	22.7	4250	-	3528	22.7
60510	850	22.7	4250	-	3570	22.7
60511	850	22.7	4250	-	3612	22.7
60512	850	22.7	4250	-	3654	22.7
60607	800	22.5	4000	-	3320	22.5
60608	800	22.5	4000	-	3360	22.5
60609	800	22.5	4000	-	3400	22.5
60610	800	22.5	4000	-	3440	22.5
60611	800	22.5	4000	-	3480	22.5
60503	850	23.9	4250	0.7	3276	22.5
60322	950	25.4	4750	1.6	3572	22.2
60606	800	22.5	4000	0.2	3280	22.0
59942	1200	30.6	6000	4.4	3360	21.8
60614	800	21.4	4000	-	3600	21.4
60615	800	21.4	4000	-	3640	21.4
60616	800	21.4	4000	-	3680	21.4
60399	900	24.3	4500	1.6	2970	21.2
60400	900	24.3	4500	1.6	3015	21.2
60401	900	24.3	4500	1.6	3060	21.2
60402	900	24.3	4500	1.6	3105	21.2
60403	900	24.3	4500	1.6	3150	21.2
60404	900	24.3	4500	1.6	3195	21.2
60405	900	24.3	4500	1.6	3240	21.2
60719	750	21.1	3750	-	3293	21.1
60720	750	21.1	3750	-	3330	21.1
60721	750	21.1	3750	-	3367	21.1
60722	750	21.1	3750	-	3404	21.1
60723	750	21.1	3750	-	3441	21.1
60724	750	21.1	3750	-	3478	21.1
60818	700	23.2	3500	1.1	2555	20.9
60845	700	20.8	3500	-	3500	20.8
60984	650	20.1	3250	-	3712	20.1
60728	750	20.1	3750	-	3626	20.1
60729	750	20.1	3750	-	3663	20.1
60730	750	20.1	3750	-	3700	20.1
60731	750	20.1	3750	-	3737	20.1
61121	600	19.9	3000	-	3510	19.9
60496	850	23.0	4250	1.6	2982	19.7
60497	850	23.0	4250	1.6	3024	19.7
60498	850	23.0	4250	1.6	3066	19.7
60499	850	23.0	4250	1.6	3108	19.7
60500	850	23.0	4250	1.6	3150	19.7
60501	850	23.0	4250	1.6	3192	19.7
60502	850	23.0	4250	1.6	3234	19.7
60839	700	19.7	3500	-	3290	19.7
60840	700	19.7	3500	-	3325	19.7
60841	700	19.7	3500	-	3360	19.7
60842	700	19.7	3500	-	3395	19.7
60843	700	19.7	3500	-	3430	19.7
60844	700	19.7	3500	-	3465	19.7
60948	650	21.5	3250	1.2	2560	19.1
60949	650	21.5	3250	1.2	2592	19.1
60850	700	18.7	3500	-	3675	18.7
60851	700	18.7	3500	-	3710	18.7
60852	700	18.7	3500	-	3745	18.7
60853	700	18.7	3500	-	3780	18.7
60971	650	18.3	3250	-	3296	18.3
60972	650	18.3	3250	-	3328	18.3
60973	650	18.3	3250	-	3360	18.3
60974	650	18.3	3250	-	3392	18.3
60975	650	18.3	3250	-	3424	18.3
60976	650	18.3	3250	-	3456	18.3
60977	650	18.3	3250	-	3488	18.3
61281	550	18.2	2750	-	3510	18.2
60617	800	21.4	4000	1.6	3720	18.1
60698	750	24.8	3750	3.5	2516	17.9
60236	1000	26.3	5000	4.3	3550	17.7
60985	650	17.4	3250	-	3744	17.4
60986	650	17.4	3250	-	3776	17.4
60987	650	17.4	3250	-	3808	17.4
60988	650	17.4	3250	-	3840	17.4
60947	650	21.5	3250	2.2	2528	17.2
61089	600	19.9	3000	1.3	2550	17.2
61090	600	19.9	3000	1.3	2580	17.2
61091	600	19.9	3000	1.3	2610	17.2
61092	600	19.9	3000	1.3	2640	17.2
61114	600	16.9	3000	-	3300	16.9
61115	600	16.9	3000	-	3330	16.9
61116	600	16.9	3000	-	3360	16.9
61117	600	16.9	3000	-	3390	16.9
61118	600	16.9	3000	-	3420	16.9
61119	600	16.9	3000	-	3450	16.9
61120	600	16.9	3000	-	3480	16.9
61093	600	19.9	3000	1.7	2670	16.5
60854	700	18.7	3500	1.1	3815	16.4
61130	600	16.0	3000	-	3780	16.0
61131	600	16.0	3000	-	3810	16.0
61132	600	16.0	3000	-	3840	16.0
61133	600	16.0	3000	-	3870	16.0
61134	600	16.0	3000	-	3900	16.0
60668	750	25.0	3750	4.7	1406	15.7
60414	900	24.1	4500	4.2	3645	15.6
60732	750	20.1	3750	2.3	3774	15.5
61006	600	15.5	3000	-	60	15.5
61273	550	15.4	2750	-	3294	15.4
61274	550	15.4	2750	-	3321	15.4
61275	550	15.4	2750	-	3348	15.4
61276	550	15.4	2750	-	3375	15.4
61277	550	15.4	2750	-	3402	15.4
61278	550	15.4	2750	-	3429	15.4
61279	550	15.4	2750	-	3456	15.4
61280	550	15.4	2750	-	3483	15.4
61245	550	18.2	2750	1.5	2538	15.3
61246	550	18.2	2750	1.5	2565	15.3
61247	550	18.2	2750	1.5	2592	15.3
61248	550	18.2	2750	1.5	2619	15.3
61249	550	18.2	2750	1.5	2646	15.3
61250	550	18.2	2750	1.5	2673	15.3
61251	550	18.2	2750	1.5	2700	15.3
61456	500	14.9	2500	-	3500	14.9
61293	550	14.7	2750	-	3834	14.7
61294	550	14.7	2750	-	3861	14.7
61295	550	14.7	2750	-	3888	14.7
61296	550	14.7	2750	-	3915	14.7
61297	550	14.7	2750	-	3942	14.7
61448	500	14.0	2500	-	3300	14.0
61449	500	14.0	2500	-	3325	14.0
61450	500	14.0	2500	-	3350	14.0
61451	500	14.0	2500	-	3375	14.0
61452	500	14.0	2500	-	3400	14.0
61453	500	14.0	2500	-	3425	14.0
61454	500	14.0	2500	-	3450	14.0
61455	500	14.0	2500	-	3475	14.0
61471	500	13.4	2500	-	3875	13.4
61472	500	13.4	2500	-	3900	13.4
61473	500	13.4	2500	-	3925	13.4
61474	500	13.4	2500	-	3950	13.4
61475	500	13.4	2500	-	3975	13.4
61476	500	13.4	2500	-	4000	13.4
61418	500	16.6	2500	1.6	2550	13.4
61419	500	16.6	2500	1.6	2575	13.4
61420	500	16.6	2500	1.6	2600	13.4
61421	500	16.6	2500	1.6	2625	13.4
61422	500	16.6	2500	1.6	2650	13.4
61423	500	16.6	2500	1.6	2675	13.4
61424	500	16.6	2500	1.6	2700	13.4
61425	500	16.6	2500	1.6	2725	13.4
61426	500	16.6	2500	1.6	2750	13.4
60989	650	17.4	3250	2.3	3872	12.8
60869	650	12.5	3250	0.2	32	12.2
61153	550	12.1	2750	-	54	12.1
61005	600	11.5	3000	-	30	11.5
61447	500	14.0	2500	1.4	3275	11.2
60868	650	10.8	3250	-	0	10.8
61318	500	10.7	2500	-	50	10.7
61152	550	10.4	2750	-	27	10.4
61298	550	14.7	2750	2.2	3969	10.3
61135	600	16.0	3000	3.8	3930	8.4
61477	500	13.4	2500	3.6	4025	6.2
60745	700	11.6	3500	2.7	0	6.2

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
59874	1250	33.8	6250	1.1	3038	31.5
59875	1250	33.8	6250	1.1	3100	31.5
59876	1250	33.4	6250	1.1	3162	31.2
59877	1250	33.4	6250	1.1	3224	31.2
59866	1250	33.8	6250	1.8	2542	30.3
59867	1250	33.8	6250	1.8	2604	30.3
59868	1250	33.8	6250	1.8	2666	30.3
59937	1200	32.4	6000	1.2	3060	30.1
59938	1200	32.4	6000	1.2	3120	30.1
59939	1200	32.1	6000	1.2	3180	29.8
59940	1200	32.1	6000	1.2	3240	29.8
59929	1200	32.4	6000	1.8	2580	28.8
59930	1200	32.4	6000	1.8	2640	28.8
59931	1200	32.4	6000	1.8	2700	28.8
59932	1200	32.4	6000	1.8	2760	28.8
59933	1200	32.4	6000	1.8	2820	28.8
59934	1200	32.4	6000	1.8	2880	28.8
60004	1150	31.1	5750	1.2	3078	28.6
60005	1150	31.1	5750	1.2	3135	28.6
60006	1150	31.1	5750	1.2	3192	28.6
60007	1150	30.7	5750	1.2	3249	28.3
59995	1150	31.1	5750	1.9	2565	27.3
59996	1150	31.1	5750	1.9	2622	27.3
59997	1150	31.1	5750	1.9	2679	27.3
59998	1150	31.1	5750	1.9	2736	27.3
59999	1150	31.1	5750	1.9	2793	27.3
60000	1150	31.1	5750	1.9	2850	27.3
60001	1150	31.1	5750	1.9	2907	27.3
60075	1100	29.7	5500	1.3	3135	27.2
60076	1100	29.7	5500	1.3	3190	27.2
60077	1100	29.7	5500	1.3	3245	27.2
60300	950	31.0	4750	2.3	2538	26.4
60065	1100	29.7	5500	2.0	2585	25.7
60066	1100	29.7	5500	2.0	2640	25.7
60067	1100	29.7	5500	2.0	2695	25.7
60068	1100	29.7	5500	2.0	2750	25.7
60069	1100	29.7	5500	2.0	2805	25.7
60070	1100	29.7	5500	2.0	2860	25.7
60071	1100	29.7	5500	2.0	2915	25.7
60151	1050	28.4	5250	1.3	3224	25.7
60138	1050	29.5	5250	2.1	2548	25.3
60216	1000	29.1	5000	2.2	2550	24.7
60390	900	29.4	4500	2.4	2565	24.5
60391	900	29.4	4500	2.4	2610	24.5
60230	1000	27.0	5000	1.4	3250	24.2
60139	1050	28.4	5250	2.1	2600	24.2
60140	1050	28.4	5250	2.1	2652	24.2
60141	1050	28.4	5250	2.1	2704	24.2
60142	1050	28.4	5250	2.1	2756	24.2
60143	1050	28.4	5250	2.1	2808	24.2
60144	1050	28.4	5250	2.1	2860	24.2
60145	1050	28.4	5250	2.1	2912	24.2
60301	950	28.8	4750	2.3	2585	24.2
60217	1000	28.1	5000	2.2	2600	23.7
60218	1000	27.0	5000	2.2	2650	22.6
60219	1000	27.0	5000	2.2	2700	22.6
60220	1000	27.0	5000	2.2	2750	22.6
60221	1000	27.0	5000	2.2	2800	22.6
60222	1000	27.0	5000	2.2	2850	22.6
60223	1000	27.0	5000	2.2	2900	22.6
60486	850	27.8	4250	2.6	2562	22.6
60487	850	27.8	4250	2.6	2604	22.6
60488	850	27.8	4250	2.6	2646	22.6
60302	950	26.7	4750	2.3	2632	22.1
60303	950	26.7	4750	2.3	2679	22.1
60389	900	29.4	4500	4.0	2520	21.4
60304	950	25.7	4750	2.3	2726	21.0
60305	950	25.7	4750	2.3	2773	21.0
60306	950	25.7	4750	2.3	2820	21.0
60307	950	25.7	4750	2.3	2867	21.0
60308	950	25.7	4750	2.3	2914	21.0
60392	900	25.6	4500	2.4	2655	20.7
60588	800	26.1	4000	2.8	2560	20.6
60589	800	26.1	4000	2.8	2600	20.6
60590	800	26.1	4000	2.8	2640	20.6
60591	800	26.1	4000	2.8	2680	20.6
60592	800	26.1	4000	2.8	2720	20.6
60489	850	25.8	4250	2.6	2688	20.6
60393	900	25.3	4500	2.4	2700	20.4
60394	900	24.3	4500	2.4	2745	19.4
60395	900	24.3	4500	2.4	2790	19.4
60396	900	24.3	4500	2.4	2835	19.4
60397	900	24.3	4500	2.4	2880	19.4
60398	900	24.3	4500	2.4	2925	19.4
60485	850	27.8	4250	4.2	2520	19.3
60699	750	24.7	3750	2.9	2553	18.8
60490	850	23.9	4250	2.6	2730	18.7
60491	850	23.9	4250	2.6	2772	18.7
60700	750	24.5	3750	2.9	2590	18.6
60701	750	24.5	3750	2.9	2627	18.6
60702	750	24.5	3750	2.9	2664	18.6
60703	750	24.5	3750	2.9	2701	18.6
60704	750	24.5	3750	2.9	2738	18.6
60705	750	24.5	3750	2.9	2775	18.6
60495	850	23.0	4250	2.4	2940	18.3
60598	800	21.6	4000	1.8	2960	18.1
60599	800	21.6	4000	1.8	3000	18.1
60600	800	21.6	4000	1.8	3040	18.1
60601	800	21.6	4000	1.8	3080	18.1
60602	800	21.6	4000	1.8	3120	18.1
60603	800	21.6	4000	1.8	3160	18.1
60604	800	21.6	4000	1.8	3200	18.1
60605	800	21.6	4000	1.8	3240	18.1
60492	850	23.0	4250	2.6	2814	17.8
60493	850	23.0	4250	2.6	2856	17.8
60494	850	23.0	4250	2.6	2898	17.8
60587	800	26.3	4000	4.5	2520	17.3
60593	800	22.7	4000	2.8	2760	17.2
60950	650	21.5	3250	2.2	2624	17.2
60594	800	22.5	4000	2.8	2800	17.0
60817	700	23.2	3500	3.1	2520	16.9
60819	700	23.0	3500	3.1	2590	16.7
60820	700	22.9	3500	3.1	2625	16.6
60821	700	22.9	3500	3.1	2660	16.6
60822	700	22.9	3500	3.1	2695	16.6
60823	700	22.9	3500	3.1	2730	16.6
60824	700	22.9	3500	3.1	2765	16.6
60825	700	22.9	3500	3.1	2800	16.6
60710	750	20.3	3750	1.9	2960	16.5
60711	750	20.3	3750	1.9	2997	16.5
60712	750	20.3	3750	1.9	3034	16.5
60713	750	20.3	3750	1.9	3071	16.5
60714	750	20.3	3750	1.9	3108	16.5
60715	750	20.3	3750	1.9	3145	16.5
60716	750	20.3	3750	1.9	3182	16.5
60717	750	20.3	3750	1.9	3219	16.5
60718	750	20.3	3750	1.9	3256	16.5
60595	800	21.9	4000	2.8	2840	16.4
60596	800	21.6	4000	2.8	2880	16.1
60597	800	21.6	4000	2.8	2920	16.1
60706	750	21.3	3750	2.9	2812	15.4
60707	750	21.1	3750	2.9	2849	15.2
60826	700	21.2	3500	3.1	2835	14.9
60830	700	18.9	3500	2.0	2975	14.9
60831	700	18.9	3500	2.0	3010	14.9
60832	700	18.9	3500	2.0	3045	14.9
60833	700	18.9	3500	2.0	3080	14.9
60834	700	18.9	3500	2.0	3115	14.9
60835	700	18.9	3500	2.0	3150	14.9
60836	700	18.9	3500	2.0	3185	14.9
60837	700	18.9	3500	2.0	3220	14.9
60838	700	18.9	3500	2.0	3255	14.9
60708	750	20.5	3750	2.9	2886	14.6
60951	650	21.4	3250	3.4	2656	14.6
60952	650	21.2	3250	3.4	2688	14.5
60953	650	21.2	3250	3.4	2720	14.5
60954	650	21.2	3250	3.4	2752	14.5
60955	650	21.2	3250	3.4	2784	14.5
60956	650	21.2	3250	3.4	2816	14.5
60957	650	21.2	3250	3.4	2848	14.5
60958	650	21.2	3250	3.4	2880	14.5
60709	750	20.3	3750	2.9	2923	14.4
60961	650	18.3	3250	2.2	2976	14.0
60513	850	22.7	4250	4.6	3696	13.6
60829	700	19.1	3500	2.9	2940	13.4
60827	700	19.7	3500	3.1	2870	13.4
60828	700	19.7	3500	3.1	2905	13.4
60960	650	18.3	3250	2.5	2944	13.3
60962	650	17.6	3250	2.2	3008	13.3
60963	650	17.6	3250	2.2	3040	13.3
60964	650	17.6	3250	2.2	3072	13.3
60965	650	17.6	3250	2.2	3104	13.3
60966	650	17.6	3250	2.2	3136	13.3
60967	650	17.6	3250	2.2	3168	13.3
60968	650	17.6	3250	2.2	3200	13.3
60969	650	17.6	3250	2.2	3232	13.3
60970	650	17.6	3250	2.2	3264	13.3
61261	550	18.0	2750	2.5	2970	12.9
61113	600	16.9	3000	2.0	3270	12.9
61088	600	19.9	3000	3.7	2520	12.5
61094	600	19.7	3000	3.7	2700	12.4
61095	600	19.6	3000	3.7	2730	12.3
61096	600	19.6	3000	3.7	2760	12.3
61097	600	19.6	3000	3.7	2790	12.3
61098	600	19.6	3000	3.7	2820	12.3
61099	600	19.6	3000	3.7	2850	12.3
61100	600	19.6	3000	3.7	2880	12.3
61101	600	19.6	3000	3.7	2910	12.3
61103	600	16.9	3000	2.3	2970	12.2
61104	600	16.9	3000	2.3	3000	12.2
61105	600	16.9	3000	2.3	3030	12.2
61252	550	18.2	2750	3.1	2727	12.0
61260	550	18.0	2750	3.1	2943	11.8
60959	650	18.5	3250	3.4	2912	11.7
61106	600	16.2	3000	2.3	3060	11.5
61107	600	16.2	3000	2.3	3090	11.5
61108	600	16.2	3000	2.3	3120	11.5
61109	600	16.2	3000	2.3	3150	11.5
61110	600	16.2	3000	2.3	3180	11.5
61111	600	16.2	3000	2.3	3210	11.5
61112	600	16.2	3000	2.3	3240	11.5
61262	550	16.4	2750	2.5	2997	11.3
61102	600	17.9	3000	3.3	2940	11.2
61434	500	16.3	2500	2.8	2950	10.7
61435	500	16.3	2500	2.8	2975	10.7
61436	500	16.3	2500	2.8	3000	10.7
61437	500	16.3	2500	2.8	3025	10.7
61427	500	16.6	2500	3.0	2775	10.6
61263	550	15.4	2750	2.5	3024	10.4
61264	550	15.4	2750	2.5	3051	10.4
61265	550	15.4	2750	2.5	3078	10.4
61253	550	18.1	2750	4.0	2754	10.1
61254	550	18.0	2750	4.0	2781	10.0
61255	550	18.0	2750	4.0	2808	10.0
61256	550	18.0	2750	4.0	2835	10.0
61257	550	18.0	2750	4.0	2862	10.0
61258	550	18.0	2750	4.0	2889	10.0
61259	550	18.0	2750	4.0	2916	10.0
61266	550	14.9	2750	2.5	3105	9.8
61267	550	14.9	2750	2.5	3132	9.8
61268	550	14.9	2750	2.5	3159	9.8
61269	550	14.9	2750	2.5	3186	9.8
61270	550	14.9	2750	2.5	3213	9.8
61271	550	14.9	2750	2.5	3240	9.8
61272	550	14.9	2750	2.5	3267	9.8
61417	500	16.6	2500	3.4	2525	9.8
61438	500	14.5	2500	2.8	3050	8.9
61439	500	14.0	2500	2.8	3075	8.4
61440	500	14.0	2500	2.8	3100	8.4
61441	500	14.0	2500	2.8	3125	8.4
61442	500	13.5	2500	2.8	3150	7.9
61443	500	13.5	2500	2.8	3175	7.9
61444	500	13.5	2500	2.8	3200	7.9
61445	500	13.5	2500	2.8	3225	7.9
61446	500	13.5	2500	2.8	3250	7.9
61428	500	16.4	2500	4.4	2800	7.6
61429	500	16.3	2500	4.4	2825	7.5
61430	500	16.3	2500	4.4	2850	7.5
61431	500	16.3	2500	4.4	2875	7.5
61432	500	16.3	2500	4.4	2900	7.5
61433	500	16.3	2500	4.4	2925	7.5

Overall score = score_ratio - 2 * repetitive DNA content