Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign84 - back to list

Summary
Image
Unique putative probes (198)
Putative probes (247)
Redundant probes (243)

Summary

`./analyse_probe_search_results : Thu Dec 1 17:04:51 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign84
  assembly      : NCBIM34
  bias          : 5prime
  chromosome    : 17
  id            : 75
  strand        : 1
  -------------

Design window length: 4457bp

All jobs for probe design are successful - total: 76
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 1507 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 1507/1507
Putative probes that are unique                 : 198/1507
Putative probes exceeding selection criteria    : 688/1507 (both unique and not unique)
Putative probes with successfully-picked primers: 688/688

Identified 243 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
71320	700	3500	-	2695
71409	650	3250	-	1856
71435	650	3250	-	2688
71436	650	3250	-	2720
71437	650	3250	-	2752
71532	600	3000	-	1860
71533	600	3000	-	1890
71560	600	3000	-	2700
71561	600	3000	-	2730
71562	600	3000	-	2760
71563	600	3000	-	2790
71564	600	3000	-	2820
71668	550	2750	-	1863
71669	550	2750	-	1890
71670	550	2750	-	1917
71671	550	2750	-	1944
71698	550	2750	-	2673
71699	550	2750	-	2700
71700	550	2750	-	2727
71701	550	2750	-	2754
71702	550	2750	-	2781
71703	550	2750	-	2808
71704	550	2750	-	2835
71705	550	2750	-	2862
71744	500	2500	-	0
71765	500	2500	-	525
71819	500	2500	-	1875
71820	500	2500	-	1900
71821	500	2500	-	1925
71822	500	2500	-	1950
71823	500	2500	-	1975
71824	500	2500	-	2000
71851	500	2500	-	2675
71852	500	2500	-	2700
71853	500	2500	-	2725
71854	500	2500	-	2750
71855	500	2500	-	2775
71856	500	2500	-	2800
71857	500	2500	-	2825
71858	500	2500	-	2850
71859	500	2500	-	2875
71860	500	2500	-	2900
71861	500	2500	-	2925
71321	700	3500	0.7	2730
71534	600	3000	0.8	1920
70739	1000	5000	1.0	0
70809	950	4750	1.1	0
70810	950	4750	1.1	47
71672	550	2750	1.1	1971
70884	900	4500	1.1	0
70885	900	4500	1.1	45
70886	900	4500	1.1	90
70887	900	4500	1.1	135
70964	850	4250	1.2	0
70965	850	4250	1.2	42
70966	850	4250	1.2	84
70967	850	4250	1.2	126
70968	850	4250	1.2	168
71390	650	3250	1.2	1248
71050	800	4000	1.2	0
71051	800	4000	1.2	40
71052	800	4000	1.2	80
71053	800	4000	1.2	120
71054	800	4000	1.2	160
71055	800	4000	1.2	200
71142	750	3750	1.3	0
71143	750	3750	1.3	37
71144	750	3750	1.3	74
71145	750	3750	1.3	111
71146	750	3750	1.3	148
71147	750	3750	1.3	185
71148	750	3750	1.3	222
71149	750	3750	1.3	259
71512	600	3000	1.3	1260
71513	600	3000	1.3	1290
71514	600	3000	1.3	1320
71056	800	4000	1.4	240
71438	650	3250	1.4	2784
71243	700	3500	1.4	0
71244	700	3500	1.4	35
71245	700	3500	1.4	70
71246	700	3500	1.4	105
71247	700	3500	1.4	140
71248	700	3500	1.4	175
71249	700	3500	1.4	210
71250	700	3500	1.4	245
71251	700	3500	1.4	280
71252	700	3500	1.4	315
71645	550	2750	1.5	1242
71646	550	2750	1.5	1269
71647	550	2750	1.5	1296
71648	550	2750	1.5	1323
71649	550	2750	1.5	1350
71351	650	3250	1.5	0
71352	650	3250	1.5	32
71353	650	3250	1.5	64
71354	650	3250	1.5	96
71355	650	3250	1.5	128
71356	650	3250	1.5	160
71357	650	3250	1.5	192
71358	650	3250	1.5	224
71359	650	3250	1.5	256
71360	650	3250	1.5	288
71361	650	3250	1.5	320
71362	650	3250	1.5	352
71363	650	3250	1.5	384
70811	950	4750	1.6	94
71794	500	2500	1.6	1250
71795	500	2500	1.6	1275
71796	500	2500	1.6	1300
71797	500	2500	1.6	1325
71798	500	2500	1.6	1350
71799	500	2500	1.6	1375
71800	500	2500	1.6	1400
71470	600	3000	1.7	0
71471	600	3000	1.7	30
71472	600	3000	1.7	60
71473	600	3000	1.7	90
71474	600	3000	1.7	120
71475	600	3000	1.7	150
71476	600	3000	1.7	180
71477	600	3000	1.7	210
71478	600	3000	1.7	240
71479	600	3000	1.7	270
71480	600	3000	1.7	300
71481	600	3000	1.7	330
71482	600	3000	1.7	360
71483	600	3000	1.7	390
71484	600	3000	1.7	420
71599	550	2750	1.8	0
71600	550	2750	1.8	27
71601	550	2750	1.8	54
71602	550	2750	1.8	81
71603	550	2750	1.8	108
71604	550	2750	1.8	135
71605	550	2750	1.8	162
71606	550	2750	1.8	189
71607	550	2750	1.8	216
71608	550	2750	1.8	243
71609	550	2750	1.8	270
71610	550	2750	1.8	297
71611	550	2750	1.8	324
71612	550	2750	1.8	351
71613	550	2750	1.8	378
71614	550	2750	1.8	405
71615	550	2750	1.8	432
71616	550	2750	1.8	459
71617	550	2750	1.8	486
70673	1050	5250	2.0	0
71745	500	2500	2.0	25
71746	500	2500	2.0	50
71747	500	2500	2.0	75
71748	500	2500	2.0	100
71749	500	2500	2.0	125
71750	500	2500	2.0	150
71751	500	2500	2.0	175
71752	500	2500	2.0	200
71753	500	2500	2.0	225
71754	500	2500	2.0	250
71755	500	2500	2.0	275
71756	500	2500	2.0	300
71757	500	2500	2.0	325
71758	500	2500	2.0	350
71759	500	2500	2.0	375
71760	500	2500	2.0	400
71761	500	2500	2.0	425
71762	500	2500	2.0	450
71763	500	2500	2.0	475
71764	500	2500	2.0	500
71825	500	2500	2.0	2025
70740	1000	5000	2.1	50
71766	500	2500	2.2	550
71150	750	3750	2.3	296
71511	600	3000	2.5	1230
71706	550	2750	2.5	2889
71253	700	3500	3.0	350
71215	750	3750	3.5	2701
71485	600	3000	3.5	450
71410	650	3250	3.5	1888
71793	500	2500	4.0	1225
71565	600	3000	4.2	2850
71277	700	3500	4.4	1190
71389	650	3250	4.5	1216
71388	650	3250	4.8	1184
71296	700	3500	4.9	1855
71862	500	2500	5.0	2950
71510	600	3000	5.2	1200
71411	650	3250	5.2	1920
71644	550	2750	5.5	1215
71643	550	2750	5.6	1188
71535	600	3000	5.7	1950
71322	700	3500	5.7	2765
71673	550	2750	6.0	1998
71791	500	2500	6.2	1175
71792	500	2500	6.2	1200
71439	650	3250	6.3	2816
71826	500	2500	6.8	2050
71707	550	2750	7.5	2916

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
70465	1250	40.8	6250	0.6	1240	39.6
70518	1200	39.2	6000	0.7	1260	37.9
70415	1300	42.5	6500	2.3	1235	37.9
70414	1300	42.2	6500	2.4	1170	37.4
70519	1200	39.2	6000	1.1	1320	37.0
70574	1150	37.6	5750	0.7	1254	36.2
70575	1150	37.6	5750	0.7	1311	36.2
70420	1300	41.1	6500	2.6	1560	35.9
70421	1300	41.1	6500	2.6	1625	35.9
70422	1300	41.1	6500	2.6	1690	35.9
70423	1300	41.1	6500	2.6	1755	35.9
70424	1300	41.1	6500	2.6	1820	35.9
70425	1300	41.1	6500	2.6	1885	35.9
70426	1300	41.1	6500	2.6	1950	35.9
70427	1300	41.1	6500	2.6	2015	35.9
70428	1300	41.1	6500	2.6	2080	35.9
70576	1150	37.6	5750	1.0	1368	35.7
70416	1300	41.1	6500	3.2	1300	34.7
70417	1300	41.1	6500	3.2	1365	34.7
70418	1300	41.1	6500	3.2	1430	34.7
70419	1300	41.1	6500	3.2	1495	34.7
70634	1100	35.9	5500	0.7	1265	34.5
70635	1100	35.9	5500	0.7	1320	34.5
70636	1100	35.9	5500	0.7	1375	34.5
70413	1300	39.2	6500	2.4	1105	34.4
70471	1250	39.6	6250	2.7	1612	34.1
70480	1250	39.6	6250	2.7	2170	34.1
70470	1250	39.6	6250	3.0	1550	33.6
70467	1250	39.6	6250	3.4	1364	32.8
70468	1250	39.6	6250	3.4	1426	32.8
70469	1250	39.6	6250	3.4	1488	32.8
70429	1300	41.1	6500	4.2	2145	32.8
70697	1050	34.3	5250	0.8	1248	32.8
70698	1050	34.3	5250	0.8	1300	32.8
70699	1050	34.3	5250	0.8	1352	32.8
70700	1050	34.3	5250	0.8	1404	32.8
70701	1050	34.3	5250	0.8	1456	32.8
70523	1200	38.0	6000	2.8	1560	32.3
70534	1200	38.0	6000	2.8	2220	32.3
70637	1100	35.9	5500	2.1	1430	31.8
70764	1000	32.7	5000	0.8	1250	31.1
70765	1000	32.7	5000	0.8	1300	31.1
70766	1000	32.7	5000	0.8	1350	31.1
70767	1000	32.7	5000	0.8	1400	31.1
70768	1000	32.7	5000	0.8	1450	31.1
70769	1000	32.7	5000	0.8	1500	31.1
70515	1200	36.1	6000	2.8	1080	30.6
70842	950	31.0	4750	0.2	1551	30.6
70580	1150	36.4	5750	3.0	1596	30.5
70592	1150	36.4	5750	3.4	2280	29.6
70919	900	29.4	4500	-	1575	29.4
70836	950	31.0	4750	0.8	1269	29.4
70837	950	31.0	4750	0.8	1316	29.4
70838	950	31.0	4750	0.8	1363	29.4
70839	950	31.0	4750	0.8	1410	29.4
70840	950	31.0	4750	0.8	1457	29.4
70841	950	31.0	4750	0.8	1504	29.4
70571	1150	34.6	5750	2.7	1083	29.2
70640	1100	34.8	5500	3.1	1595	28.6
70641	1100	34.8	5500	3.1	1650	28.6
70653	1100	34.8	5500	3.1	2310	28.6
70920	900	29.4	4500	0.6	1620	28.3
71001	850	27.8	4250	-	1554	27.8
71002	850	27.8	4250	-	1596	27.8
71003	850	27.8	4250	-	1638	27.8
70912	900	29.4	4500	0.9	1260	27.6
70913	900	29.4	4500	0.9	1305	27.6
70914	900	29.4	4500	0.9	1350	27.6
70915	900	29.4	4500	0.9	1395	27.6
70916	900	29.4	4500	0.9	1440	27.6
70917	900	29.4	4500	0.9	1485	27.6
70918	900	29.4	4500	0.9	1530	27.6
70631	1100	33.1	5500	2.8	1100	27.5
71025	850	27.2	4250	-	2562	27.2
70718	1050	33.2	5250	3.2	2340	26.8
71089	800	26.1	4000	-	1560	26.1
71090	800	26.1	4000	-	1600	26.1
71091	800	26.1	4000	-	1640	26.1
71092	800	26.1	4000	-	1680	26.1
70994	850	27.8	4250	0.9	1260	25.9
70995	850	27.8	4250	0.9	1302	25.9
70996	850	27.8	4250	0.9	1344	25.9
70997	850	27.8	4250	0.9	1386	25.9
70998	850	27.8	4250	0.9	1428	25.9
70999	850	27.8	4250	0.9	1470	25.9
71000	850	27.8	4250	0.9	1512	25.9
70694	1050	31.6	5250	3.0	1092	25.7
71114	800	25.6	4000	-	2560	25.6
71115	800	25.6	4000	-	2600	25.6
70597	1150	34.2	5750	4.4	2565	25.4
71093	800	26.1	4000	0.6	1720	24.9
70787	1000	31.6	5000	3.4	2400	24.8
71184	750	24.5	3750	-	1554	24.5
71185	750	24.5	3750	-	1591	24.5
71186	750	24.5	3750	-	1628	24.5
71187	750	24.5	3750	-	1665	24.5
71188	750	24.5	3750	-	1702	24.5
71189	750	24.5	3750	-	1739	24.5
71004	850	27.8	4250	1.8	1680	24.2
71081	800	26.1	4000	1.0	1240	24.1
71082	800	26.1	4000	1.0	1280	24.1
71083	800	26.1	4000	1.0	1320	24.1
71084	800	26.1	4000	1.0	1360	24.1
71085	800	26.1	4000	1.0	1400	24.1
71086	800	26.1	4000	1.0	1440	24.1
71087	800	26.1	4000	1.0	1480	24.1
71088	800	26.1	4000	1.0	1520	24.1
71211	750	24.0	3750	-	2553	24.0
71212	750	24.0	3750	-	2590	24.0
71213	750	24.0	3750	-	2627	24.0
71214	750	24.0	3750	-	2664	24.0
70761	1000	30.1	5000	3.1	1100	23.9
70861	950	30.1	4750	3.6	2444	22.9
71288	700	22.9	3500	-	1575	22.9
71289	700	22.9	3500	-	1610	22.9
71290	700	22.9	3500	-	1645	22.9
71291	700	22.9	3500	-	1680	22.9
71292	700	22.9	3500	-	1715	22.9
71293	700	22.9	3500	-	1750	22.9
71294	700	22.9	3500	-	1785	22.9
71316	700	22.4	3500	-	2555	22.4
71317	700	22.4	3500	-	2590	22.4
71318	700	22.4	3500	-	2625	22.4
71319	700	22.4	3500	-	2660	22.4
70940	900	28.8	4500	3.2	2520	22.4
71176	750	24.5	3750	1.1	1258	22.4
71177	750	24.5	3750	1.1	1295	22.4
71178	750	24.5	3750	1.1	1332	22.4
71179	750	24.5	3750	1.1	1369	22.4
71180	750	24.5	3750	1.1	1406	22.4
71181	750	24.5	3750	1.1	1443	22.4
71182	750	24.5	3750	1.1	1480	22.4
71183	750	24.5	3750	1.1	1517	22.4
71116	800	25.6	4000	1.9	2640	21.9
70832	950	28.6	4750	3.4	1081	21.9
71190	750	24.5	3750	1.5	1776	21.6
71295	700	22.9	3500	0.7	1820	21.4
70722	1050	31.2	5250	5.0	2548	21.3
71400	650	21.2	3250	-	1568	21.2
71401	650	21.2	3250	-	1600	21.2
71402	650	21.2	3250	-	1632	21.2
71403	650	21.2	3250	-	1664	21.2
71404	650	21.2	3250	-	1696	21.2
71405	650	21.2	3250	-	1728	21.2
71406	650	21.2	3250	-	1760	21.2
71407	650	21.2	3250	-	1792	21.2
71408	650	21.2	3250	-	1824	21.2
71431	650	20.8	3250	-	2560	20.8
71432	650	20.8	3250	-	2592	20.8
71433	650	20.8	3250	-	2624	20.8
71434	650	20.8	3250	-	2656	20.8
71279	700	22.9	3500	1.1	1260	20.6
71280	700	22.9	3500	1.1	1295	20.6
71281	700	22.9	3500	1.1	1330	20.6
71282	700	22.9	3500	1.1	1365	20.6
71283	700	22.9	3500	1.1	1400	20.6
71284	700	22.9	3500	1.1	1435	20.6
71285	700	22.9	3500	1.1	1470	20.6
71286	700	22.9	3500	1.1	1505	20.6
71287	700	22.9	3500	1.1	1540	20.6
71026	850	27.2	4250	3.4	2604	20.4
71559	600	20.0	3000	-	2670	20.0
70908	900	27.1	4500	3.7	1080	19.8
71522	600	19.6	3000	-	1560	19.6
71523	600	19.6	3000	-	1590	19.6
71524	600	19.6	3000	-	1620	19.6
71525	600	19.6	3000	-	1650	19.6
71526	600	19.6	3000	-	1680	19.6
71527	600	19.6	3000	-	1710	19.6
71528	600	19.6	3000	-	1740	19.6
71529	600	19.6	3000	-	1770	19.6
71530	600	19.6	3000	-	1800	19.6
71531	600	19.6	3000	-	1830	19.6
71555	600	19.2	3000	-	2550	19.2
71556	600	19.2	3000	-	2580	19.2
71557	600	19.2	3000	-	2610	19.2
71558	600	19.2	3000	-	2640	19.2
70462	1250	28.4	6250	4.7	1054	19.0
71391	650	21.2	3250	1.2	1280	18.8
71392	650	21.2	3250	1.2	1312	18.8
71393	650	21.2	3250	1.2	1344	18.8
71394	650	21.2	3250	1.2	1376	18.8
71395	650	21.2	3250	1.2	1408	18.8
71396	650	21.2	3250	1.2	1440	18.8
71397	650	21.2	3250	1.2	1472	18.8
71398	650	21.2	3250	1.2	1504	18.8
71399	650	21.2	3250	1.2	1536	18.8
70990	850	25.6	4250	3.6	1092	18.3
71657	550	18.0	2750	-	1566	18.0
71658	550	18.0	2750	-	1593	18.0
71659	550	18.0	2750	-	1620	18.0
71660	550	18.0	2750	-	1647	18.0
71661	550	18.0	2750	-	1674	18.0
71662	550	18.0	2750	-	1701	18.0
71663	550	18.0	2750	-	1728	18.0
71664	550	18.0	2750	-	1755	18.0
71665	550	18.0	2750	-	1782	18.0
71666	550	18.0	2750	-	1809	18.0
71667	550	18.0	2750	-	1836	18.0
71694	550	17.6	2750	-	2565	17.6
71695	550	17.6	2750	-	2592	17.6
71696	550	17.6	2750	-	2619	17.6
71697	550	17.6	2750	-	2646	17.6
71515	600	19.6	3000	1.3	1350	16.9
71516	600	19.6	3000	1.3	1380	16.9
71517	600	19.6	3000	1.3	1410	16.9
71518	600	19.6	3000	1.3	1440	16.9
71519	600	19.6	3000	1.3	1470	16.9
71520	600	19.6	3000	1.3	1500	16.9
71521	600	19.6	3000	1.3	1530	16.9
71807	500	16.3	2500	-	1575	16.3
71808	500	16.3	2500	-	1600	16.3
71809	500	16.3	2500	-	1625	16.3
71810	500	16.3	2500	-	1650	16.3
71811	500	16.3	2500	-	1675	16.3
71812	500	16.3	2500	-	1700	16.3
71813	500	16.3	2500	-	1725	16.3
71814	500	16.3	2500	-	1750	16.3
71815	500	16.3	2500	-	1775	16.3
71816	500	16.3	2500	-	1800	16.3
71817	500	16.3	2500	-	1825	16.3
71818	500	16.3	2500	-	1850	16.3
71846	500	16.0	2500	-	2550	16.0
71847	500	16.0	2500	-	2575	16.0
71848	500	16.0	2500	-	2600	16.0
71849	500	16.0	2500	-	2625	16.0
71850	500	16.0	2500	-	2650	16.0
71077	800	24.1	4000	4.1	1080	15.8
70969	850	22.6	4250	3.6	210	15.3
71806	500	16.3	2500	0.6	1550	15.1
71650	550	18.0	2750	1.5	1377	15.1
71651	550	18.0	2750	1.5	1404	15.1
71652	550	18.0	2750	1.5	1431	15.1
71653	550	18.0	2750	1.5	1458	15.1
71654	550	18.0	2750	1.5	1485	15.1
71655	550	18.0	2750	1.5	1512	15.1
71656	550	18.0	2750	1.5	1539	15.1
71693	550	17.6	2750	2.0	2538	13.6
71801	500	16.3	2500	1.6	1425	13.1
71802	500	16.3	2500	1.6	1450	13.1
71803	500	16.3	2500	1.6	1475	13.1
71804	500	16.3	2500	1.6	1500	13.1
71805	500	16.3	2500	1.6	1525	13.1
71274	700	21.1	3500	4.4	1085	12.2
70497	1200	19.4	6000	4.4	0	10.6
70553	1150	18.6	5750	4.6	57	9.4
70613	1100	17.8	5500	4.8	110	8.2

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
70464	1250	40.8	6250	2.5	1178	35.9
70466	1250	40.8	6250	3.4	1302	34.1
70472	1250	39.6	6250	2.7	1674	34.1
70473	1250	39.6	6250	2.7	1736	34.1
70474	1250	39.6	6250	2.7	1798	34.1
70475	1250	39.6	6250	2.7	1860	34.1
70476	1250	39.6	6250	2.7	1922	34.1
70477	1250	39.6	6250	2.7	1984	34.1
70478	1250	39.6	6250	2.7	2046	34.1
70479	1250	39.6	6250	2.7	2108	34.1
70517	1200	39.2	6000	2.6	1200	34.0
70463	1250	37.7	6250	2.5	1116	32.7
70524	1200	38.0	6000	2.8	1620	32.3
70525	1200	38.0	6000	2.8	1680	32.3
70526	1200	38.0	6000	2.8	1740	32.3
70527	1200	38.0	6000	2.8	1800	32.3
70528	1200	38.0	6000	2.8	1860	32.3
70529	1200	38.0	6000	2.8	1920	32.3
70530	1200	38.0	6000	2.8	1980	32.3
70531	1200	38.0	6000	2.8	2040	32.3
70532	1200	38.0	6000	2.8	2100	32.3
70533	1200	38.0	6000	2.8	2160	32.3
70573	1150	37.6	5750	2.7	1197	32.2
70520	1200	39.0	6000	3.5	1380	32.0
70516	1200	36.1	6000	2.6	1140	31.0
70521	1200	38.0	6000	3.5	1440	31.0
70522	1200	38.0	6000	3.5	1500	31.0
70581	1150	36.4	5750	3.0	1653	30.5
70582	1150	36.4	5750	3.0	1710	30.5
70583	1150	36.4	5750	3.0	1767	30.5
70584	1150	36.4	5750	3.0	1824	30.5
70585	1150	36.4	5750	3.0	1881	30.5
70586	1150	36.4	5750	3.0	1938	30.5
70587	1150	36.4	5750	3.0	1995	30.5
70588	1150	36.4	5750	3.0	2052	30.5
70589	1150	36.4	5750	3.0	2109	30.5
70590	1150	36.4	5750	3.0	2166	30.5
70591	1150	36.4	5750	3.0	2223	30.5
70633	1100	35.9	5500	2.8	1210	30.3
70577	1150	37.3	5750	3.7	1425	30.0
70572	1150	34.6	5750	2.7	1140	29.2
70578	1150	36.4	5750	3.7	1482	29.1
70579	1150	36.4	5750	3.7	1539	29.1
70642	1100	34.8	5500	3.1	1705	28.6
70643	1100	34.8	5500	3.1	1760	28.6
70644	1100	34.8	5500	3.1	1815	28.6
70645	1100	34.8	5500	3.1	1870	28.6
70646	1100	34.8	5500	3.1	1925	28.6
70647	1100	34.8	5500	3.1	1980	28.6
70648	1100	34.8	5500	3.1	2035	28.6
70649	1100	34.8	5500	3.1	2090	28.6
70650	1100	34.8	5500	3.1	2145	28.6
70651	1100	34.8	5500	3.1	2200	28.6
70652	1100	34.8	5500	3.1	2255	28.6
70696	1050	34.3	5250	3.0	1196	28.4
70638	1100	35.7	5500	3.8	1485	28.1
70632	1100	33.1	5500	2.8	1155	27.5
70639	1100	34.8	5500	3.8	1540	27.2
70703	1050	33.2	5250	3.2	1560	26.8
70704	1050	33.2	5250	3.2	1612	26.8
70705	1050	33.2	5250	3.2	1664	26.8
70706	1050	33.2	5250	3.2	1716	26.8
70707	1050	33.2	5250	3.2	1768	26.8
70708	1050	33.2	5250	3.2	1820	26.8
70709	1050	33.2	5250	3.2	1872	26.8
70710	1050	33.2	5250	3.2	1924	26.8
70711	1050	33.2	5250	3.2	1976	26.8
70712	1050	33.2	5250	3.2	2028	26.8
70713	1050	33.2	5250	3.2	2080	26.8
70714	1050	33.2	5250	3.2	2132	26.8
70715	1050	33.2	5250	3.2	2184	26.8
70716	1050	33.2	5250	3.2	2236	26.8
70717	1050	33.2	5250	3.2	2288	26.8
70763	1000	32.7	5000	3.1	1200	26.5
70702	1050	34.3	5250	4.0	1508	26.3
70835	950	31.0	4750	2.4	1222	26.2
70695	1050	31.6	5250	3.0	1144	25.7
70770	1000	32.7	5000	3.7	1550	25.3
70771	1000	31.6	5000	3.4	1600	24.8
70772	1000	31.6	5000	3.4	1650	24.8
70773	1000	31.6	5000	3.4	1700	24.8
70774	1000	31.6	5000	3.4	1750	24.8
70775	1000	31.6	5000	3.4	1800	24.8
70776	1000	31.6	5000	3.4	1850	24.8
70777	1000	31.6	5000	3.4	1900	24.8
70778	1000	31.6	5000	3.4	1950	24.8
70779	1000	31.6	5000	3.4	2000	24.8
70780	1000	31.6	5000	3.4	2050	24.8
70781	1000	31.6	5000	3.4	2100	24.8
70782	1000	31.6	5000	3.4	2150	24.8
70783	1000	31.6	5000	3.4	2200	24.8
70784	1000	31.6	5000	3.4	2250	24.8
70785	1000	31.6	5000	3.4	2300	24.8
70786	1000	31.6	5000	3.4	2350	24.8
70834	950	31.0	4750	3.3	1175	24.5
70843	950	31.0	4750	3.5	1598	24.1
70762	1000	30.1	5000	3.1	1150	23.9
70719	1050	33.2	5250	4.9	2392	23.5
70658	1100	32.7	5500	4.6	2585	23.5
70844	950	30.1	4750	3.6	1645	22.9
70845	950	30.1	4750	3.6	1692	22.9
70846	950	30.1	4750	3.6	1739	22.9
70847	950	30.1	4750	3.6	1786	22.9
70848	950	30.1	4750	3.6	1833	22.9
70849	950	30.1	4750	3.6	1880	22.9
70850	950	30.1	4750	3.6	1927	22.9
70851	950	30.1	4750	3.6	1974	22.9
70852	950	30.1	4750	3.6	2021	22.9
70853	950	30.1	4750	3.6	2068	22.9
70854	950	30.1	4750	3.6	2115	22.9
70855	950	30.1	4750	3.6	2162	22.9
70856	950	30.1	4750	3.6	2209	22.9
70857	950	30.1	4750	3.6	2256	22.9
70858	950	30.1	4750	3.6	2303	22.9
70859	950	30.1	4750	3.6	2350	22.9
70860	950	30.1	4750	3.6	2397	22.9
70911	900	29.4	4500	3.3	1215	22.7
70910	900	29.2	4500	3.4	1170	22.3
70833	950	28.6	4750	3.3	1128	22.1
70921	900	29.4	4500	3.8	1665	21.9
70723	1050	31.2	5250	4.9	2600	21.5
70724	1050	31.2	5250	4.9	2652	21.5
70993	850	27.8	4250	3.2	1218	21.4
70922	900	28.5	4500	3.8	1710	20.9
70923	900	28.5	4500	3.8	1755	20.9
70924	900	28.5	4500	3.8	1800	20.9
70925	900	28.5	4500	3.8	1845	20.9
70926	900	28.5	4500	3.8	1890	20.9
70927	900	28.5	4500	3.8	1935	20.9
70928	900	28.5	4500	3.8	1980	20.9
70929	900	28.5	4500	3.8	2025	20.9
70930	900	28.5	4500	3.8	2070	20.9
70931	900	28.5	4500	3.8	2115	20.9
70932	900	28.5	4500	3.8	2160	20.9
70933	900	28.5	4500	3.8	2205	20.9
70934	900	28.5	4500	3.8	2250	20.9
70935	900	28.5	4500	3.8	2295	20.9
70936	900	28.5	4500	3.8	2340	20.9
70937	900	28.5	4500	3.8	2385	20.9
70938	900	28.5	4500	3.8	2430	20.9
70939	900	28.5	4500	3.8	2475	20.9
70992	850	27.8	4250	3.6	1176	20.5
71024	850	27.2	4250	3.4	2520	20.4
70909	900	27.1	4500	3.4	1125	20.2
70941	900	28.8	4500	4.4	2565	20.0
71005	850	27.8	4250	4.0	1722	19.8
71006	850	26.9	4250	4.0	1764	18.9
71007	850	26.9	4250	4.0	1806	18.9
71008	850	26.9	4250	4.0	1848	18.9
71009	850	26.9	4250	4.0	1890	18.9
71010	850	26.9	4250	4.0	1932	18.9
71011	850	26.9	4250	4.0	1974	18.9
71012	850	26.9	4250	4.0	2016	18.9
71013	850	26.9	4250	4.0	2058	18.9
71014	850	26.9	4250	4.0	2100	18.9
71015	850	26.9	4250	4.0	2142	18.9
71016	850	26.9	4250	4.0	2184	18.9
71017	850	26.9	4250	4.0	2226	18.9
71018	850	26.9	4250	4.0	2268	18.9
71019	850	26.9	4250	4.0	2310	18.9
71020	850	26.9	4250	4.0	2352	18.9
71021	850	26.9	4250	4.0	2394	18.9
71022	850	26.9	4250	4.0	2436	18.9
71023	850	26.9	4250	4.0	2478	18.9
71080	800	26.1	4000	3.9	1200	18.4
71113	800	25.6	4000	3.6	2520	18.4
70991	850	25.6	4250	3.6	1134	18.3
71175	750	24.5	3750	3.2	1221	18.1
71094	800	26.1	4000	4.2	1760	17.6
71278	700	22.9	3500	2.9	1225	17.2
71095	800	25.3	4000	4.2	1800	16.8
71096	800	25.3	4000	4.2	1840	16.8
71097	800	25.3	4000	4.2	1880	16.8
71098	800	25.3	4000	4.2	1920	16.8
71099	800	25.3	4000	4.2	1960	16.8
71100	800	25.3	4000	4.2	2000	16.8
71101	800	25.3	4000	4.2	2040	16.8
71102	800	25.3	4000	4.2	2080	16.8
71103	800	25.3	4000	4.2	2120	16.8
71104	800	25.3	4000	4.2	2160	16.8
71105	800	25.3	4000	4.2	2200	16.8
71106	800	25.3	4000	4.2	2240	16.8
71107	800	25.3	4000	4.2	2280	16.8
71108	800	25.3	4000	4.2	2320	16.8
71109	800	25.3	4000	4.2	2360	16.8
71110	800	25.3	4000	4.2	2400	16.8
71111	800	25.3	4000	4.2	2440	16.8
71112	800	25.3	4000	4.2	2480	16.8
71078	800	24.1	4000	3.9	1120	16.3
71079	800	24.1	4000	3.9	1160	16.3
71174	750	24.5	3750	4.1	1184	16.2
71191	750	24.5	3750	4.5	1813	15.4
71210	750	23.9	3750	4.4	2516	15.1
71192	750	23.7	3750	4.5	1850	14.7
71193	750	23.7	3750	4.5	1887	14.7
71194	750	23.7	3750	4.5	1924	14.7
71195	750	23.7	3750	4.5	1961	14.7
71196	750	23.7	3750	4.5	1998	14.7
71197	750	23.7	3750	4.5	2035	14.7
71198	750	23.7	3750	4.5	2072	14.7
71199	750	23.7	3750	4.5	2109	14.7
71200	750	23.7	3750	4.5	2146	14.7
71201	750	23.7	3750	4.5	2183	14.7
71202	750	23.7	3750	4.5	2220	14.7
71203	750	23.7	3750	4.5	2257	14.7
71204	750	23.7	3750	4.5	2294	14.7
71205	750	23.7	3750	4.5	2331	14.7
71206	750	23.7	3750	4.5	2368	14.7
71207	750	23.7	3750	4.5	2405	14.7
71208	750	23.7	3750	4.5	2442	14.7
71209	750	23.7	3750	4.5	2479	14.7
71430	650	20.8	3250	3.2	2528	14.4
71172	750	22.6	3750	4.1	1110	14.3
71173	750	22.6	3750	4.1	1147	14.3
71315	700	22.4	3500	4.1	2520	14.2
71297	700	22.7	3500	4.9	1890	13.0
71298	700	22.2	3500	4.9	1925	12.4
71299	700	22.2	3500	4.9	1960	12.4
71300	700	22.2	3500	4.9	1995	12.4
71301	700	22.2	3500	4.9	2030	12.4
71302	700	22.2	3500	4.9	2065	12.4
71303	700	22.2	3500	4.9	2100	12.4
71304	700	22.2	3500	4.9	2135	12.4
71305	700	22.2	3500	4.9	2170	12.4
71306	700	22.2	3500	4.9	2205	12.4
71307	700	22.2	3500	4.9	2240	12.4
71308	700	22.2	3500	4.9	2275	12.4
71309	700	22.2	3500	4.9	2310	12.4
71310	700	22.2	3500	4.9	2345	12.4
71311	700	22.2	3500	4.9	2380	12.4
71312	700	22.2	3500	4.9	2415	12.4
71313	700	22.2	3500	4.9	2450	12.4
71314	700	22.2	3500	4.9	2485	12.4
71275	700	21.1	3500	4.4	1120	12.2
71276	700	21.1	3500	4.4	1155	12.2
71385	650	19.6	3250	4.8	1088	10.0
71386	650	19.6	3250	4.8	1120	10.0
71387	650	19.6	3250	4.8	1152	10.0
71554	600	19.2	3000	4.8	2520	9.6
70552	1150	18.6	5750	4.6	0	9.4
70611	1100	18.4	5500	4.8	0	8.8
70612	1100	17.8	5500	4.8	55	8.2
71845	500	16.0	2500	4.8	2525	6.4

Overall score = score_ratio - 2 * repetitive DNA content