Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign91 - back to list

Summary
Image
Unique putative probes (408)
Putative probes (292)
Redundant probes (0)

Summary

`./analyse_probe_search_results : Tue Dec 20 11:40:02 2005`

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign91
  assembly      : NCBIM34
  bias          : 3prime
  chromosome    : 5
  id            : 82
  strand        : 1
  -------------

Design window length: 2501bp

All jobs for probe design are successful - total: 35
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 700 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 3prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 700/700
Putative probes that are unique                 : 408/700
Putative probes exceeding selection criteria    : 700/700 (both unique and not unique)
Putative probes with successfully-picked primers: 652/700

Identified 0 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Unique putative probes

Putative probe ID	Length	Actual	%rep DNA	Distance
77495	1300	6500	-	910
77496	1300	6500	-	975
77497	1300	6500	-	1040
77498	1300	6500	-	1105
77499	1300	6500	-	1170
77515	1250	6250	-	930
77516	1250	6250	-	992
77517	1250	6250	-	1054
77518	1250	6250	-	1116
77519	1250	6250	-	1178
77520	1250	6250	-	1240
77536	1200	6000	-	900
77537	1200	6000	-	960
77538	1200	6000	-	1020
77539	1200	6000	-	1080
77540	1200	6000	-	1140
77541	1200	6000	-	1200
77542	1200	6000	-	1260
77559	1150	5750	-	912
77560	1150	5750	-	969
77561	1150	5750	-	1026
77562	1150	5750	-	1083
77563	1150	5750	-	1140
77564	1150	5750	-	1197
77565	1150	5750	-	1254
77566	1150	5750	-	1311
77583	1100	5500	-	880
77584	1100	5500	-	935
77585	1100	5500	-	990
77586	1100	5500	-	1045
77587	1100	5500	-	1100
77588	1100	5500	-	1155
77589	1100	5500	-	1210
77590	1100	5500	-	1265
77591	1100	5500	-	1320
77592	1100	5500	-	1375
77610	1050	5250	-	884
77611	1050	5250	-	936
77612	1050	5250	-	988
77613	1050	5250	-	1040
77614	1050	5250	-	1092
77615	1050	5250	-	1144
77616	1050	5250	-	1196
77617	1050	5250	-	1248
77618	1050	5250	-	1300
77619	1050	5250	-	1352
77620	1050	5250	-	1404
77639	1000	5000	-	900
77640	1000	5000	-	950
77641	1000	5000	-	1000
77642	1000	5000	-	1050
77643	1000	5000	-	1100
77644	1000	5000	-	1150
77645	1000	5000	-	1200
77646	1000	5000	-	1250
77647	1000	5000	-	1300
77648	1000	5000	-	1350
77649	1000	5000	-	1400
77650	1000	5000	-	1450
77651	1000	5000	-	1500
77671	950	4750	-	893
77672	950	4750	-	940
77673	950	4750	-	987
77674	950	4750	-	1034
77675	950	4750	-	1081
77676	950	4750	-	1128
77677	950	4750	-	1175
77678	950	4750	-	1222
77679	950	4750	-	1269
77680	950	4750	-	1316
77681	950	4750	-	1363
77682	950	4750	-	1410
77683	950	4750	-	1457
77684	950	4750	-	1504
77685	950	4750	-	1551
77686	900	4500	-	0
77687	900	4500	-	45
77706	900	4500	-	900
77707	900	4500	-	945
77708	900	4500	-	990
77709	900	4500	-	1035
77710	900	4500	-	1080
77711	900	4500	-	1125
77712	900	4500	-	1170
77713	900	4500	-	1215
77714	900	4500	-	1260
77715	900	4500	-	1305
77716	900	4500	-	1350
77717	900	4500	-	1395
77718	900	4500	-	1440
77719	900	4500	-	1485
77720	900	4500	-	1530
77721	900	4500	-	1575
77722	850	4250	-	0
77723	850	4250	-	42
77724	850	4250	-	84
77743	850	4250	-	882
77744	850	4250	-	924
77745	850	4250	-	966
77746	850	4250	-	1008
77747	850	4250	-	1050
77748	850	4250	-	1092
77749	850	4250	-	1134
77750	850	4250	-	1176
77751	850	4250	-	1218
77752	850	4250	-	1260
77753	850	4250	-	1302
77754	850	4250	-	1344
77755	850	4250	-	1386
77756	850	4250	-	1428
77757	850	4250	-	1470
77758	850	4250	-	1512
77759	850	4250	-	1554
77760	850	4250	-	1596
77761	850	4250	-	1638
77762	800	4000	-	0
77763	800	4000	-	40
77764	800	4000	-	80
77765	800	4000	-	120
77784	800	4000	-	880
77785	800	4000	-	920
77786	800	4000	-	960
77787	800	4000	-	1000
77788	800	4000	-	1040
77789	800	4000	-	1080
77790	800	4000	-	1120
77791	800	4000	-	1160
77792	800	4000	-	1200
77793	800	4000	-	1240
77794	800	4000	-	1280
77795	800	4000	-	1320
77796	800	4000	-	1360
77797	800	4000	-	1400
77798	800	4000	-	1440
77799	800	4000	-	1480
77800	800	4000	-	1520
77801	800	4000	-	1560
77802	800	4000	-	1600
77803	800	4000	-	1640
77804	800	4000	-	1680
77805	750	3750	-	0
77806	750	3750	-	37
77807	750	3750	-	74
77808	750	3750	-	111
77809	750	3750	-	148
77810	750	3750	-	185
77829	750	3750	-	888
77830	750	3750	-	925
77831	750	3750	-	962
77832	750	3750	-	999
77833	750	3750	-	1036
77834	750	3750	-	1073
77835	750	3750	-	1110
77836	750	3750	-	1147
77837	750	3750	-	1184
77838	750	3750	-	1221
77839	750	3750	-	1258
77840	750	3750	-	1295
77841	750	3750	-	1332
77842	750	3750	-	1369
77843	750	3750	-	1406
77844	750	3750	-	1443
77845	750	3750	-	1480
77846	750	3750	-	1517
77847	750	3750	-	1554
77848	750	3750	-	1591
77849	750	3750	-	1628
77850	750	3750	-	1665
77851	750	3750	-	1702
77852	750	3750	-	1739
77853	700	3500	-	0
77854	700	3500	-	35
77855	700	3500	-	70
77856	700	3500	-	105
77857	700	3500	-	140
77858	700	3500	-	175
77859	700	3500	-	210
77860	700	3500	-	245
77879	700	3500	-	910
77880	700	3500	-	945
77881	700	3500	-	980
77882	700	3500	-	1015
77883	700	3500	-	1050
77884	700	3500	-	1085
77885	700	3500	-	1120
77886	700	3500	-	1155
77887	700	3500	-	1190
77888	700	3500	-	1225
77889	700	3500	-	1260
77890	700	3500	-	1295
77891	700	3500	-	1330
77892	700	3500	-	1365
77893	700	3500	-	1400
77894	700	3500	-	1435
77895	700	3500	-	1470
77896	700	3500	-	1505
77897	700	3500	-	1540
77898	700	3500	-	1575
77899	700	3500	-	1610
77900	700	3500	-	1645
77901	700	3500	-	1680
77902	700	3500	-	1715
77903	700	3500	-	1750
77904	700	3500	-	1785
77905	650	3250	-	0
77906	650	3250	-	32
77907	650	3250	-	64
77908	650	3250	-	96
77909	650	3250	-	128
77910	650	3250	-	160
77911	650	3250	-	192
77912	650	3250	-	224
77913	650	3250	-	256
77914	650	3250	-	288
77933	650	3250	-	896
77934	650	3250	-	928
77935	650	3250	-	960
77936	650	3250	-	992
77937	650	3250	-	1024
77938	650	3250	-	1056
77939	650	3250	-	1088
77940	650	3250	-	1120
77941	650	3250	-	1152
77942	650	3250	-	1184
77943	650	3250	-	1216
77944	650	3250	-	1248
77945	650	3250	-	1280
77946	650	3250	-	1312
77947	650	3250	-	1344
77948	650	3250	-	1376
77949	650	3250	-	1408
77950	650	3250	-	1440
77951	650	3250	-	1472
77952	650	3250	-	1504
77953	650	3250	-	1536
77954	650	3250	-	1568
77955	650	3250	-	1600
77956	650	3250	-	1632
77957	650	3250	-	1664
77958	650	3250	-	1696
77959	650	3250	-	1728
77960	650	3250	-	1760
77961	650	3250	-	1792
77962	650	3250	-	1824
77963	600	3000	-	0
77964	600	3000	-	30
77965	600	3000	-	60
77966	600	3000	-	90
77967	600	3000	-	120
77968	600	3000	-	150
77969	600	3000	-	180
77970	600	3000	-	210
77971	600	3000	-	240
77972	600	3000	-	270
77973	600	3000	-	300
77974	600	3000	-	330
77993	600	3000	-	900
77994	600	3000	-	930
77995	600	3000	-	960
77996	600	3000	-	990
77997	600	3000	-	1020
77998	600	3000	-	1050
77999	600	3000	-	1080
78000	600	3000	-	1110
78001	600	3000	-	1140
78002	600	3000	-	1170
78003	600	3000	-	1200
78004	600	3000	-	1230
78005	600	3000	-	1260
78006	600	3000	-	1290
78007	600	3000	-	1320
78008	600	3000	-	1350
78009	600	3000	-	1380
78010	600	3000	-	1410
78011	600	3000	-	1440
78012	600	3000	-	1470
78013	600	3000	-	1500
78014	600	3000	-	1530
78015	600	3000	-	1560
78016	600	3000	-	1590
78017	600	3000	-	1620
78018	600	3000	-	1650
78019	600	3000	-	1680
78020	600	3000	-	1710
78021	600	3000	-	1740
78022	600	3000	-	1770
78023	600	3000	-	1800
78024	600	3000	-	1830
78025	600	3000	-	1860
78026	600	3000	-	1890
78027	550	2750	-	0
78028	550	2750	-	27
78029	550	2750	-	54
78030	550	2750	-	81
78031	550	2750	-	108
78032	550	2750	-	135
78033	550	2750	-	162
78034	550	2750	-	189
78035	550	2750	-	216
78036	550	2750	-	243
78037	550	2750	-	270
78038	550	2750	-	297
78039	550	2750	-	324
78040	550	2750	-	351
78041	550	2750	-	378
78060	550	2750	-	891
78061	550	2750	-	918
78062	550	2750	-	945
78063	550	2750	-	972
78064	550	2750	-	999
78065	550	2750	-	1026
78066	550	2750	-	1053
78067	550	2750	-	1080
78068	550	2750	-	1107
78069	550	2750	-	1134
78070	550	2750	-	1161
78071	550	2750	-	1188
78072	550	2750	-	1215
78073	550	2750	-	1242
78074	550	2750	-	1269
78075	550	2750	-	1296
78076	550	2750	-	1323
78077	550	2750	-	1350
78078	550	2750	-	1377
78079	550	2750	-	1404
78080	550	2750	-	1431
78081	550	2750	-	1458
78082	550	2750	-	1485
78083	550	2750	-	1512
78084	550	2750	-	1539
78085	550	2750	-	1566
78086	550	2750	-	1593
78087	550	2750	-	1620
78088	550	2750	-	1647
78089	550	2750	-	1674
78090	550	2750	-	1701
78091	550	2750	-	1728
78092	550	2750	-	1755
78093	550	2750	-	1782
78094	550	2750	-	1809
78095	550	2750	-	1836
78096	550	2750	-	1863
78097	550	2750	-	1890
78098	550	2750	-	1917
78099	550	2750	-	1944
78100	500	2500	-	0
78101	500	2500	-	25
78102	500	2500	-	50
78103	500	2500	-	75
78104	500	2500	-	100
78105	500	2500	-	125
78106	500	2500	-	150
78107	500	2500	-	175
78108	500	2500	-	200
78109	500	2500	-	225
78110	500	2500	-	250
78111	500	2500	-	275
78112	500	2500	-	300
78113	500	2500	-	325
78114	500	2500	-	350
78115	500	2500	-	375
78116	500	2500	-	400
78117	500	2500	-	425
78136	500	2500	-	900
78137	500	2500	-	925
78138	500	2500	-	950
78139	500	2500	-	975
78140	500	2500	-	1000
78141	500	2500	-	1025
78142	500	2500	-	1050
78143	500	2500	-	1075
78144	500	2500	-	1100
78145	500	2500	-	1125
78146	500	2500	-	1150
78147	500	2500	-	1175
78148	500	2500	-	1200
78149	500	2500	-	1225
78150	500	2500	-	1250
78151	500	2500	-	1275
78152	500	2500	-	1300
78153	500	2500	-	1325
78154	500	2500	-	1350
78155	500	2500	-	1375
78156	500	2500	-	1400
78157	500	2500	-	1425
78158	500	2500	-	1450
78159	500	2500	-	1475
78160	500	2500	-	1500
78161	500	2500	-	1525
78162	500	2500	-	1550
78163	500	2500	-	1575
78164	500	2500	-	1600
78165	500	2500	-	1625
78166	500	2500	-	1650
78167	500	2500	-	1675
78168	500	2500	-	1700
78169	500	2500	-	1725
78170	500	2500	-	1750
78171	500	2500	-	1775
78172	500	2500	-	1800
78173	500	2500	-	1825
78174	500	2500	-	1850
78175	500	2500	-	1875
78176	500	2500	-	1900
78177	500	2500	-	1925
78178	500	2500	-	1950
78179	500	2500	-	1975
78180	500	2500	-	2000

Putative probes

Putative probe ID	Length	Score ratio	Actual	%rep DNA	Distance	Overall
77481	1300	41.7	6500	-	0	41.7
77482	1300	41.7	6500	-	65	41.7
77483	1300	41.7	6500	-	130	41.7
77484	1300	41.7	6500	-	195	41.7
77485	1300	41.7	6500	-	260	41.7
77486	1300	41.7	6500	-	325	41.7
77487	1300	41.7	6500	-	390	41.7
77488	1300	41.7	6500	-	455	41.7
77489	1300	41.7	6500	-	520	41.7
77490	1300	41.7	6500	-	585	41.7
77491	1300	41.7	6500	-	650	41.7
77492	1300	41.7	6500	-	715	41.7
77493	1300	41.7	6500	-	780	41.7
77494	1300	41.7	6500	-	845	41.7
77500	1250	40.1	6250	-	0	40.1
77501	1250	40.1	6250	-	62	40.1
77502	1250	40.1	6250	-	124	40.1
77503	1250	40.1	6250	-	186	40.1
77504	1250	40.1	6250	-	248	40.1
77505	1250	40.1	6250	-	310	40.1
77506	1250	40.1	6250	-	372	40.1
77507	1250	40.1	6250	-	434	40.1
77508	1250	40.1	6250	-	496	40.1
77509	1250	40.1	6250	-	558	40.1
77510	1250	40.1	6250	-	620	40.1
77511	1250	40.1	6250	-	682	40.1
77512	1250	40.1	6250	-	744	40.1
77513	1250	40.1	6250	-	806	40.1
77514	1250	40.1	6250	-	868	40.1
77521	1200	38.5	6000	-	0	38.5
77522	1200	38.5	6000	-	60	38.5
77523	1200	38.5	6000	-	120	38.5
77524	1200	38.5	6000	-	180	38.5
77525	1200	38.5	6000	-	240	38.5
77526	1200	38.5	6000	-	300	38.5
77527	1200	38.5	6000	-	360	38.5
77528	1200	38.5	6000	-	420	38.5
77529	1200	38.5	6000	-	480	38.5
77530	1200	38.5	6000	-	540	38.5
77531	1200	38.5	6000	-	600	38.5
77532	1200	38.5	6000	-	660	38.5
77533	1200	38.5	6000	-	720	38.5
77534	1200	38.5	6000	-	780	38.5
77535	1200	38.5	6000	-	840	38.5
77543	1150	36.9	5750	-	0	36.9
77544	1150	36.9	5750	-	57	36.9
77545	1150	36.9	5750	-	114	36.9
77546	1150	36.9	5750	-	171	36.9
77547	1150	36.9	5750	-	228	36.9
77548	1150	36.9	5750	-	285	36.9
77549	1150	36.9	5750	-	342	36.9
77550	1150	36.9	5750	-	399	36.9
77551	1150	36.9	5750	-	456	36.9
77552	1150	36.9	5750	-	513	36.9
77553	1150	36.9	5750	-	570	36.9
77554	1150	36.9	5750	-	627	36.9
77555	1150	36.9	5750	-	684	36.9
77556	1150	36.9	5750	-	741	36.9
77557	1150	36.9	5750	-	798	36.9
77558	1150	36.9	5750	-	855	36.9
77567	1100	35.3	5500	-	0	35.3
77568	1100	35.3	5500	-	55	35.3
77569	1100	35.3	5500	-	110	35.3
77570	1100	35.3	5500	-	165	35.3
77571	1100	35.3	5500	-	220	35.3
77572	1100	35.3	5500	-	275	35.3
77573	1100	35.3	5500	-	330	35.3
77574	1100	35.3	5500	-	385	35.3
77575	1100	35.3	5500	-	440	35.3
77576	1100	35.3	5500	-	495	35.3
77577	1100	35.3	5500	-	550	35.3
77578	1100	35.3	5500	-	605	35.3
77579	1100	35.3	5500	-	660	35.3
77580	1100	35.3	5500	-	715	35.3
77581	1100	35.3	5500	-	770	35.3
77582	1100	35.3	5500	-	825	35.3
77593	1050	33.7	5250	-	0	33.7
77594	1050	33.7	5250	-	52	33.7
77595	1050	33.7	5250	-	104	33.7
77596	1050	33.7	5250	-	156	33.7
77597	1050	33.7	5250	-	208	33.7
77598	1050	33.7	5250	-	260	33.7
77599	1050	33.7	5250	-	312	33.7
77600	1050	33.7	5250	-	364	33.7
77601	1050	33.7	5250	-	416	33.7
77602	1050	33.7	5250	-	468	33.7
77603	1050	33.7	5250	-	520	33.7
77604	1050	33.7	5250	-	572	33.7
77605	1050	33.7	5250	-	624	33.7
77606	1050	33.7	5250	-	676	33.7
77607	1050	33.7	5250	-	728	33.7
77608	1050	33.7	5250	-	780	33.7
77609	1050	33.7	5250	-	832	33.7
77621	1000	32.1	5000	-	0	32.1
77622	1000	32.1	5000	-	50	32.1
77623	1000	32.1	5000	-	100	32.1
77624	1000	32.1	5000	-	150	32.1
77625	1000	32.1	5000	-	200	32.1
77626	1000	32.1	5000	-	250	32.1
77627	1000	32.1	5000	-	300	32.1
77628	1000	32.1	5000	-	350	32.1
77629	1000	32.1	5000	-	400	32.1
77630	1000	32.1	5000	-	450	32.1
77631	1000	32.1	5000	-	500	32.1
77632	1000	32.1	5000	-	550	32.1
77633	1000	32.1	5000	-	600	32.1
77634	1000	32.1	5000	-	650	32.1
77635	1000	32.1	5000	-	700	32.1
77636	1000	32.1	5000	-	750	32.1
77637	1000	32.1	5000	-	800	32.1
77638	1000	32.1	5000	-	850	32.1
77652	950	30.4	4750	-	0	30.4
77653	950	30.4	4750	-	47	30.4
77654	950	30.4	4750	-	94	30.4
77655	950	30.4	4750	-	141	30.4
77656	950	30.4	4750	-	188	30.4
77657	950	30.4	4750	-	235	30.4
77658	950	30.4	4750	-	282	30.4
77659	950	30.4	4750	-	329	30.4
77660	950	30.4	4750	-	376	30.4
77661	950	30.4	4750	-	423	30.4
77662	950	30.4	4750	-	470	30.4
77663	950	30.4	4750	-	517	30.4
77664	950	30.4	4750	-	564	30.4
77665	950	30.4	4750	-	611	30.4
77666	950	30.4	4750	-	658	30.4
77667	950	30.4	4750	-	705	30.4
77668	950	30.4	4750	-	752	30.4
77669	950	30.4	4750	-	799	30.4
77670	950	30.4	4750	-	846	30.4
77688	900	28.8	4500	-	90	28.8
77689	900	28.8	4500	-	135	28.8
77690	900	28.8	4500	-	180	28.8
77691	900	28.8	4500	-	225	28.8
77692	900	28.8	4500	-	270	28.8
77693	900	28.8	4500	-	315	28.8
77694	900	28.8	4500	-	360	28.8
77695	900	28.8	4500	-	405	28.8
77696	900	28.8	4500	-	450	28.8
77697	900	28.8	4500	-	495	28.8
77698	900	28.8	4500	-	540	28.8
77699	900	28.8	4500	-	585	28.8
77700	900	28.8	4500	-	630	28.8
77701	900	28.8	4500	-	675	28.8
77702	900	28.8	4500	-	720	28.8
77703	900	28.8	4500	-	765	28.8
77704	900	28.8	4500	-	810	28.8
77705	900	28.8	4500	-	855	28.8
77725	850	27.2	4250	-	126	27.2
77726	850	27.2	4250	-	168	27.2
77727	850	27.2	4250	-	210	27.2
77728	850	27.2	4250	-	252	27.2
77729	850	27.2	4250	-	294	27.2
77730	850	27.2	4250	-	336	27.2
77731	850	27.2	4250	-	378	27.2
77732	850	27.2	4250	-	420	27.2
77733	850	27.2	4250	-	462	27.2
77734	850	27.2	4250	-	504	27.2
77735	850	27.2	4250	-	546	27.2
77736	850	27.2	4250	-	588	27.2
77737	850	27.2	4250	-	630	27.2
77738	850	27.2	4250	-	672	27.2
77739	850	27.2	4250	-	714	27.2
77740	850	27.2	4250	-	756	27.2
77741	850	27.2	4250	-	798	27.2
77742	850	27.2	4250	-	840	27.2
77766	800	25.6	4000	-	160	25.6
77767	800	25.6	4000	-	200	25.6
77768	800	25.6	4000	-	240	25.6
77769	800	25.6	4000	-	280	25.6
77770	800	25.6	4000	-	320	25.6
77771	800	25.6	4000	-	360	25.6
77772	800	25.6	4000	-	400	25.6
77773	800	25.6	4000	-	440	25.6
77774	800	25.6	4000	-	480	25.6
77775	800	25.6	4000	-	520	25.6
77776	800	25.6	4000	-	560	25.6
77777	800	25.6	4000	-	600	25.6
77778	800	25.6	4000	-	640	25.6
77779	800	25.6	4000	-	680	25.6
77780	800	25.6	4000	-	720	25.6
77781	800	25.6	4000	-	760	25.6
77782	800	25.6	4000	-	800	25.6
77783	800	25.6	4000	-	840	25.6
77811	750	24.0	3750	-	222	24.0
77812	750	24.0	3750	-	259	24.0
77813	750	24.0	3750	-	296	24.0
77814	750	24.0	3750	-	333	24.0
77815	750	24.0	3750	-	370	24.0
77816	750	24.0	3750	-	407	24.0
77817	750	24.0	3750	-	444	24.0
77818	750	24.0	3750	-	481	24.0
77819	750	24.0	3750	-	518	24.0
77820	750	24.0	3750	-	555	24.0
77821	750	24.0	3750	-	592	24.0
77822	750	24.0	3750	-	629	24.0
77823	750	24.0	3750	-	666	24.0
77824	750	24.0	3750	-	703	24.0
77825	750	24.0	3750	-	740	24.0
77826	750	24.0	3750	-	777	24.0
77827	750	24.0	3750	-	814	24.0
77828	750	24.0	3750	-	851	24.0
77878	700	23.3	3500	-	875	23.3
77861	700	22.4	3500	-	280	22.4
77862	700	22.4	3500	-	315	22.4
77863	700	22.4	3500	-	350	22.4
77864	700	22.4	3500	-	385	22.4
77865	700	22.4	3500	-	420	22.4
77866	700	22.4	3500	-	455	22.4
77867	700	22.4	3500	-	490	22.4
77868	700	22.4	3500	-	525	22.4
77869	700	22.4	3500	-	560	22.4
77870	700	22.4	3500	-	595	22.4
77871	700	22.4	3500	-	630	22.4
77872	700	22.4	3500	-	665	22.4
77873	700	22.4	3500	-	700	22.4
77874	700	22.4	3500	-	735	22.4
77875	700	22.4	3500	-	770	22.4
77876	700	22.4	3500	-	805	22.4
77877	700	22.4	3500	-	840	22.4
77915	650	20.8	3250	-	320	20.8
77916	650	20.8	3250	-	352	20.8
77917	650	20.8	3250	-	384	20.8
77918	650	20.8	3250	-	416	20.8
77919	650	20.8	3250	-	448	20.8
77920	650	20.8	3250	-	480	20.8
77921	650	20.8	3250	-	512	20.8
77922	650	20.8	3250	-	544	20.8
77923	650	20.8	3250	-	576	20.8
77924	650	20.8	3250	-	608	20.8
77925	650	20.8	3250	-	640	20.8
77926	650	20.8	3250	-	672	20.8
77927	650	20.8	3250	-	704	20.8
77928	650	20.8	3250	-	736	20.8
77929	650	20.8	3250	-	768	20.8
77930	650	20.8	3250	-	800	20.8
77931	650	20.8	3250	-	832	20.8
77932	650	20.8	3250	-	864	20.8
77975	600	19.2	3000	-	360	19.2
77976	600	19.2	3000	-	390	19.2
77977	600	19.2	3000	-	420	19.2
77978	600	19.2	3000	-	450	19.2
77979	600	19.2	3000	-	480	19.2
77980	600	19.2	3000	-	510	19.2
77981	600	19.2	3000	-	540	19.2
77982	600	19.2	3000	-	570	19.2
77983	600	19.2	3000	-	600	19.2
77984	600	19.2	3000	-	630	19.2
77985	600	19.2	3000	-	660	19.2
77986	600	19.2	3000	-	690	19.2
77987	600	19.2	3000	-	720	19.2
77988	600	19.2	3000	-	750	19.2
77989	600	19.2	3000	-	780	19.2
77990	600	19.2	3000	-	810	19.2
77991	600	19.2	3000	-	840	19.2
77992	600	19.2	3000	-	870	19.2
78042	550	17.6	2750	-	405	17.6
78043	550	17.6	2750	-	432	17.6
78044	550	17.6	2750	-	459	17.6
78045	550	17.6	2750	-	486	17.6
78046	550	17.6	2750	-	513	17.6
78047	550	17.6	2750	-	540	17.6
78048	550	17.6	2750	-	567	17.6
78049	550	17.6	2750	-	594	17.6
78050	550	17.6	2750	-	621	17.6
78051	550	17.6	2750	-	648	17.6
78052	550	17.6	2750	-	675	17.6
78053	550	17.6	2750	-	702	17.6
78054	550	17.6	2750	-	729	17.6
78055	550	17.6	2750	-	756	17.6
78056	550	17.6	2750	-	783	17.6
78057	550	17.6	2750	-	810	17.6
78058	550	17.6	2750	-	837	17.6
78059	550	17.6	2750	-	864	17.6
78135	500	16.7	2500	-	875	16.7
78118	500	16.0	2500	-	450	16.0
78119	500	16.0	2500	-	475	16.0
78120	500	16.0	2500	-	500	16.0
78121	500	16.0	2500	-	525	16.0
78122	500	16.0	2500	-	550	16.0
78123	500	16.0	2500	-	575	16.0
78124	500	16.0	2500	-	600	16.0
78125	500	16.0	2500	-	625	16.0
78126	500	16.0	2500	-	650	16.0
78127	500	16.0	2500	-	675	16.0
78128	500	16.0	2500	-	700	16.0
78129	500	16.0	2500	-	725	16.0
78130	500	16.0	2500	-	750	16.0
78131	500	16.0	2500	-	775	16.0
78132	500	16.0	2500	-	800	16.0
78133	500	16.0	2500	-	825	16.0
78134	500	16.0	2500	-	850	16.0

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

No probes

Overall score = score_ratio - 2 * repetitive DNA content