Automated design of genomic Southern blot probes

Mike DR Croning, David G Fricker, Noboru H Komiyama and Seth GN Grant

Design Case - ProbeDesign98 - back to list

Summary

./analyse_probe_search_results : Wed Feb 1 11:25:12 2006

Connected to host: host, as user: user
Database       : southern_blot_designs

Fetched probe design: ProbeDesign98
  assembly      : NCBIM34
  bias          : 5prime
  chromosome    : 9
  id            : 101
  strand        : 1
  -------------

Design window length: 1501bp

All jobs for probe design are successful - total: 15
Fetched conf : Exonerate mouse_NCBIM34 (id: 1)
  Genome file(s) at: /blastdb/Mouse/NCBIM34/softmasked_dusted
Fetched 289 putative probes to analyse

Selection criteria to rank probes:
  Minimum score ratio             : 10
  (i.e. self-hit must score at least 10 times higher than the next best hit)
  Maximum percent repetitive bases: 5
  Favour probes to the 5prime end of design window
  ------------------------

Putative probes without hits                    : 0
Putative probes with single_self_hit            : 289/289
Putative probes that are unique                 : 289/289
Putative probes exceeding selection criteria    : 289/289 (both unique and not unique)
Putative probes with successfully-picked primers: 286/289

Identified 0 'redundant' probes exceeding selection criteria
(but wholly contained by other probes, and higher repetitive DNA content)

Image

Unique (yellow), passed (green) and failed putative probes in the design window

Chart

Unique putative probes

Putative probe IDLengthActual%rep DNADistance
9520913006500-0
9521013006500-65
9521113006500-130
9521213006500-195
9521312506250-0
9521412506250-62
9521512506250-124
9521612506250-186
9521712506250-248
9521812006000-0
9521912006000-60
9522012006000-120
9522112006000-180
9522212006000-240
9522312006000-300
9522411505750-0
9522511505750-57
9522611505750-114
9522711505750-171
9522811505750-228
9522911505750-285
9523011505750-342
9523111005500-0
9523211005500-55
9523311005500-110
9523411005500-165
9523511005500-220
9523611005500-275
9523711005500-330
9523811005500-385
9523910505250-0
9524010505250-52
9524110505250-104
9524210505250-156
9524310505250-208
9524410505250-260
9524510505250-312
9524610505250-364
9524710505250-416
9524810005000-0
9524910005000-50
9525010005000-100
9525110005000-150
9525210005000-200
9525310005000-250
9525410005000-300
9525510005000-350
9525610005000-400
9525710005000-450
9525810005000-500
952599504750-0
952609504750-47
952619504750-94
952629504750-141
952639504750-188
952649504750-235
952659504750-282
952669504750-329
952679504750-376
952689504750-423
952699504750-470
952709504750-517
952719004500-0
952729004500-45
952739004500-90
952749004500-135
952759004500-180
952769004500-225
952779004500-270
952789004500-315
952799004500-360
952809004500-405
952819004500-450
952829004500-495
952839004500-540
952849004500-585
952858504250-0
952868504250-42
952878504250-84
952888504250-126
952898504250-168
952908504250-210
952918504250-252
952928504250-294
952938504250-336
952948504250-378
952958504250-420
952968504250-462
952978504250-504
952988504250-546
952998504250-588
953008504250-630
953018004000-0
953028004000-40
953038004000-80
953048004000-120
953058004000-160
953068004000-200
953078004000-240
953088004000-280
953098004000-320
953108004000-360
953118004000-400
953128004000-440
953138004000-480
953148004000-520
953158004000-560
953168004000-600
953178004000-640
953188004000-680
953197503750-0
953207503750-37
953217503750-74
953227503750-111
953237503750-148
953247503750-185
953257503750-222
953267503750-259
953277503750-296
953287503750-333
953297503750-370
953307503750-407
953317503750-444
953327503750-481
953337503750-518
953347503750-555
953357503750-592
953367503750-629
953377503750-666
953387503750-703
953397503750-740
953407003500-0
953417003500-35
953427003500-70
953437003500-105
953447003500-140
953457003500-175
953467003500-210
953477003500-245
953487003500-280
953497003500-315
953507003500-350
953517003500-385
953527003500-420
953537003500-455
953547003500-490
953557003500-525
953567003500-560
953577003500-595
953587003500-630
953597003500-665
953607003500-700
953617003500-735
953627003500-770
953636503250-0
953646503250-32
953656503250-64
953666503250-96
953676503250-128
953686503250-160
953696503250-192
953706503250-224
953716503250-256
953726503250-288
953736503250-320
953746503250-352
953756503250-384
953766503250-416
953776503250-448
953786503250-480
953796503250-512
953806503250-544
953816503250-576
953826503250-608
953836503250-640
953846503250-672
953856503250-704
953866503250-736
953876503250-768
953886503250-800
953896503250-832
953906003000-0
953916003000-30
953926003000-60
953936003000-90
953946003000-120
953956003000-150
953966003000-180
953976003000-210
953986003000-240
953996003000-270
954006003000-300
954016003000-330
954026003000-360
954036003000-390
954046003000-420
954056003000-450
954066003000-480
954076003000-510
954086003000-540
954096003000-570
954106003000-600
954116003000-630
954126003000-660
954136003000-690
954146003000-720
954156003000-750
954166003000-780
954176003000-810
954186003000-840
954196003000-870
954206003000-900
954215502750-0
954225502750-27
954235502750-54
954245502750-81
954255502750-108
954265502750-135
954275502750-162
954285502750-189
954295502750-216
954305502750-243
954315502750-270
954325502750-297
954335502750-324
954345502750-351
954355502750-378
954365502750-405
954375502750-432
954385502750-459
954395502750-486
954405502750-513
954415502750-540
954425502750-567
954435502750-594
954445502750-621
954455502750-648
954465502750-675
954475502750-702
954485502750-729
954495502750-756
954505502750-783
954515502750-810
954525502750-837
954535502750-864
954545502750-891
954555502750-918
954565502750-945
954575002500-0
954585002500-25
954595002500-50
954605002500-75
954615002500-100
954625002500-125
954635002500-150
954645002500-175
954655002500-200
954665002500-225
954675002500-250
954685002500-275
954695002500-300
954705002500-325
954715002500-350
954725002500-375
954735002500-400
954745002500-425
954755002500-450
954765002500-475
954775002500-500
954785002500-525
954795002500-550
954805002500-575
954815002500-600
954825002500-625
954835002500-650
954845002500-675
954855002500-700
954865002500-725
954875002500-750
954885002500-775
954895002500-800
954905002500-825
954915002500-850
954925002500-875
954935002500-900
954945002500-925
954955002500-950
954965002500-975
954975002500-1000

Putative probes

No probes

Overall score = score_ratio - 2 * repetitive DNA content

Redundant probes

No probes

Overall score = score_ratio - 2 * repetitive DNA content

© G2C 2014. The Genes to Cognition Programme received funding from The Wellcome Trust and the EU FP7 Framework Programmes:
EUROSPIN (FP7-HEALTH-241498), SynSys (FP7-HEALTH-242167) and GENCODYS (FP7-HEALTH-241995).

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